                         SEQUENCE LISTING



<110>  Liverpool School of Tropical Medicine

 

<120>  CHIMERIC FC RECEPTOR BINDING PROTEINS AND USES THEREOF



<130>  P254103WO



<160>  60    



<170>  PatentIn version 3.5



<210>  1

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  Reference protein: Hexa-Fc or HexaGard



<400>  1



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Cys Leu Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Asn Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Cys Tyr 

                245                 





<210>  2

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  C309L/C575A



<400>  2



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Asn Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  3

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  D221N/C309L/C575A



<400>  3



Asn Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Asn Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  4

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  C309L/N563A/C575A



<400>  4



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Ala Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  5

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  D221N/C309L/N297A/N563A/C575A



<400>  5



Asn Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Ala Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  6

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  D221N/C309L/N563A/C575A



<400>  6



Asn Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Ala Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  7

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  - D221N/C309L/N297A/C575A



<400>  7



Asn Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Asn Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  8

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  C309L/N297A/C575A



<400>  8



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Asn Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  9

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  D221N/N297A/N563A/C575A



<400>  9



Asn Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Cys Leu Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Ala Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  10

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  N297A/N563A/C575A



<400>  10



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Cys Leu Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Ala Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  11

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  C309L



<400>  11



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Asn Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Cys Tyr 

                245                 





<210>  12

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding seq 1 (Hexa-Fc)



<400>  12

gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac      240



cgtgtggtca gcgtcctcac cgtctgcctc caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacaacgt gtccctggtc      720



atgtccgaca cagctggcac ctgctactga                                       750





<210>  13

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding C309L/C575A



<400>  13

gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacaacgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  14

<211>  18

<212>  PRT

<213>  Homo sapiens



<400>  14



Pro Thr Leu Tyr Asn Val Ser Leu Val Met Ser Asp Thr Ala Gly Thr 

1               5                   10                  15      





Cys Tyr 

        





<210>  15

<211>  18

<212>  PRT

<213>  Homo sapiens



<400>  15



Pro Thr His Val Asn Val Ser Val Val Met Ala Gln Val Asp Gly Thr 

1               5                   10                  15      





Cys Tyr 

        





<210>  16

<211>  4

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  spacer region



<400>  16



Leu Val Leu Gly 

1               





<210>  17

<211>  23

<212>  PRT

<213>  Homo sapiens



<400>  17



Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 

1               5                   10                  15      





Pro Glu Leu Leu Gly Gly Pro 

            20              





<210>  18

<211>  10

<212>  PRT

<213>  Homo sapiens



<400>  18



Asp Lys Thr His Thr Cys Pro Pro Cys Pro 

1               5                   10  





<210>  19

<211>  19

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  19



Val Pro Ser Thr Pro Pro Thr Pro Ser Pro Ser Thr Pro Pro Thr Pro 

1               5                   10                  15      





Ser Pro Ser 

            





<210>  20

<211>  6

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  20



Val Pro Pro Pro Pro Pro 

1               5       





<210>  21

<211>  15

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  21



Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 

1               5                   10                  15  





<210>  22

<211>  12

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  22



Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 

1               5                   10          





<210>  23

<211>  12

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  23



Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 

1               5                   10          





<210>  24

<211>  4

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  24



Cys Pro Pro Cys 

1               





<210>  25

<211>  4

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  25



Cys Pro Ser Cys 

1               





<210>  26

<211>  4

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  26



Ser Pro Pro Cys 

1               





<210>  27

<211>  10

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  hinge region



<400>  27



Asp Lys Thr His Thr Cys Pro Pro Cys Pro 

1               5                   10  





<210>  28

<211>  18

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  exemplary protein of the invention



<400>  28



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro 

        





<210>  29

<211>  19

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Forward Primer



<400>  29

accctgcttg ctcaactct                                                    19





<210>  30

<211>  29

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Reverse Primer



<400>  30

ggccagctag ctcagtaggc ggtgccagc                                         29





<210>  31

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Forward Primer



<400>  31

tcaccgtctt gcaccaggac t                                                 21





<210>  32

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Reverse Primer



<400>  32

agtcctggtg caagacggtg a                                                 21





<210>  33

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  N297A Forward Primer



<400>  33

gagcagtacg ccagcacgta c                                                 21





<210>  34

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  N297A Reverse Primer



<400>  34

ctcgtcatgc ggtcgtgcat g                                                 21





<210>  35

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  N563A Forward Primer



<400>  35

ccctgtacgc cgtgtccctg                                                   20





<210>  36

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  N563A Reverse Primer



<400>  36

gggacatgcg gcacagggac                                                   20





<210>  37

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  D221N Forward Primer



<400>  37

gttagatcta acaaaactca c                                                 21





<210>  38

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  D221N Reverse Primer



<400>  38

caatctagat tgttttgagt g                                                 21





<210>  39

<211>  19

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  C575A Forward Primer



<400>  39

accctgcttg ctcaactct                                                    19





<210>  40

<211>  29

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  C575A reverse primer



<400>  40

ggccagctag ctcagtaggc ggtgccagc                                         29





<210>  41

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  C309L Forward Primer



<400>  41

tcaccgtctt gcaccaggac t                                                 21





<210>  42

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  C309L Reverse Primer



<400>  42

agtcctggtg caagacggtg a                                                 21





<210>  43

<211>  19

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Overlap PCR Forward Primer



<400>  43

accctgcttg ctcaactct                                                    19





<210>  44

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  overlap PCR Reverse Primer



<400>  44

tggtttgtcc aaactcatca a                                                 21





<210>  45

<211>  18

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  modified  "tailpiece"



<400>  45



Pro Thr His Val Asn Val Ser Val Val Met Ala Glu Val Asp Gly Thr 

1               5                   10                  15      





Cys Tyr 

        





<210>  46

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding D221N/C309L/C575A



<400>  46

aacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacaacgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  47

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding C309L/N563A/C575A



<400>  47

gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacgccgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  48

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding D221N/C309L/N297A/N563A/C575A



<400>  48

aacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacgc cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacgccgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  49

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding D221N/C309L/N563A/C575A



<400>  49

aacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacgccgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  50

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding D221N/C309L/N297A/C575A



<400>  50

aacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacgc cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacaacgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  51

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding C309L/N297A/C575A



<400>  51

gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacgc cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacaacgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  52

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding D221N/N297A/N563A/C575A



<400>  52

aacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacgc cagcacgtac      240



cgtgtggtca gcgtcctcac cgtctgcctc caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacgccgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  53

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding N297A/N563A/C575A



<400>  53

gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacgc cagcacgtac      240



cgtgtggtca gcgtcctcac cgtctgcctc caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacgccgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  54

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding C309L



<400>  54

gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacaacgt gtccctggtc      720



atgtccgaca cagctggcac ctgctactga                                       750





<210>  55

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  C309L/N297A/N563A/C575A



<400>  55



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Ala Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  56

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding C309L/N297A/N563A/C575A



<400>  56

gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacgc cagcacgtac      240



cgtgtggtca gcgtcctcac cgtcttgcac caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacgccgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  57

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  N297A/C575A



<400>  57



Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Cys Leu Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Asn Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  58

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding N297A/C575A



<400>  58

gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacgc cagcacgtac      240



cgtgtggtca gcgtcctcac cgtctgcctc caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacaacgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





<210>  59

<211>  249

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  D221N/C575A



<400>  59



Asn Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 

1               5                   10                  15      





Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 

            20                  25                  30          





Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 

        35                  40                  45              





Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 

    50                  55                  60                  





His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 

65                  70                  75                  80  





Arg Val Val Ser Val Leu Thr Val Cys Leu Gln Asp Trp Leu Asn Gly 

                85                  90                  95      





Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 

            100                 105                 110         





Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 

        115                 120                 125             





Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 

    130                 135                 140                 





Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 

145                 150                 155                 160 





Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 

                165                 170                 175     





Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 

            180                 185                 190         





Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 

        195                 200                 205             





His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 

    210                 215                 220                 





Pro Gly Lys Leu Val Leu Gly Pro Pro Leu Tyr Asn Val Ser Leu Val 

225                 230                 235                 240 





Met Ser Asp Thr Ala Gly Thr Ala Tyr 

                245                 





<210>  60

<211>  750

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  nucleic acid encoding D221N/C575A



<400>  60

aacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc       60



ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca      120



tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac      180



ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac      240



cgtgtggtca gcgtcctcac cgtctgcctc caggactggc tgaatggcaa ggagtacaag      300



tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa      360



gggcagcccc gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag      420



aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag      480



tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc      540



gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg      600



aacgtcttct catgctccgt gatgcacgag gctctgcaca accactacac gcagaagagc      660



ctctccctgt ctccgggtaa attagtccta ggaccccccc tgtacaacgt gtccctggtc      720



atgtccgaca cagctggcac cgcctactga                                       750





