                         SEQUENCE LISTING

<110>  Jiangtao Ren
 
<120>  Engineered chimeric guide RNA and uses thereof

<130>  BH-PCT-006

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-crRNA

<400>  1

gaatttctac taagtgtaga tctgatggtc catgtctgtt actctttttt              50                                            


<210>  2
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-crRNA

<400>  2

gaatttctac taagtgtaga tctaggaata ttgaaggggg caggtttttt              50                                               


<210>  3
<211>  76
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GRIN2b-crRNA

<400>  3

gaatttctac taagtgtaga tgtgctcaat gaaaggagat aaggtttttt               50                                                                                       


<210>  4
<211>  76
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-caRNA

<400>  4

gaatttctac taagtgtaga tctgatggtc catgtctgtt actcaatttc tactaagtgt    60

agattttttt aaaaaa                                                    76


<210>  5
<211>  76
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-caRNA

<400>  5

gaatttctac taagtgtaga tctaggaata ttgaaggggg caggaatttc tactaagtgt    60

agattttttt aaaaaa                                                    76


<210>  6
<211>  76
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GRIN2b-caRNA

<400>  6

gaatttctac taagtgtaga tgtgctcaat gaaaggagat aaggaatttc tactaagtgt    60

agattttttt aaaaaa                                                    76


<210>  7
<211>  118
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-csRNA

<400>  7

gccagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg    60

gctggaaata atttctacta agtgtagatc tgatggtcca tgtctgttac tctttttt     118


<210>  8
<211>  118
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-csRNA

<400>  8

gccagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg    60

gctggaaata atttctacta agtgtagatc taggaatatt gaagggggca ggtttttt     118


<210>  9
<211>  124
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GRIN2b-csRNA

<400>  9   

gccagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg    60

gctggaaata atttctacta agtgtagatg tgctcaatga aaggagataa ggttttttaa   120

aaaa                                                                124


<210>  10
<211>  144
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-catRNA

<400>  10 

gccagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg    60

gctggaaata atttctacta agtgtagatc tgatggtcca tgtctgttac tcaatttcta   120

ctaagtgtag atttttttaa aaaa                                          144


<210>  11
<211>  144
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-catRNA

<400>  11

gccagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg    60

gctggaaata atttctacta agtgtagatc taggaatatt gaagggggca ggaatttcta   120

ctaagtgtag atttttttaa aaaa                                          144


<210>  12
<211>  144
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GRIN2b-catRNA

<400>  12

gccagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg    60

gctggaaata atttctacta agtgtagatg tgctcaatga aaggagataa ggaatttcta   120

ctaagtgtag atttttttaa aaaa                                          144


<210>  13
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-1

<400>  13

tcagcaccat ttgttaaaga cac                                             23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-2

<400>  14

cgcgcgaaaa gccggggcgc ctg                                              23


<210>  15
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-3

<400>  15

tgagagccct tgagtaaagt cct                                              23
 

<210>  16
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-1

<400>  16

tgacctccca aacagctaca tat                                              23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-2

<400>  17

ctgctccctc ctcgccaatg ccc                                              23


<210>  18
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-3

<400>  18

tccccaaatc actgtggatt ttg                                               23


<210>  19
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-24bp

<400>  19

ctgatggtcc atgtctgtta ctcg                                              24


<210>  20
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-20bp

<400>  20

ctgatggtcc atgtctgtta                                                   20


<210>  21
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-18bp

<400>  21

ctgatggtcc atgtctgt                                                      18


<210>  22
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-17bp

<400>  22

ctgatggtcc atgtctg                                                       17


<210>  23
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-16bp

<400>  23

ctgatggtcc atgtct                                                        16


<210>  24
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-15bp

<400>  24

ctgatggtcc atgtc                                                         15


<210>  25
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNMT1-14bp

<400>  25

ctgatggtcc atgt                                                          14


<210>  26
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-24bp

<400>  26

ctaggaatat tgaagggggc aggg                                               24


<210>  27
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-20bp

<400>  27

ctaggaatat tgaagggggc                                                    20


<210>  28
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-18bp

<400>  28

ctaggaatat tgaagggg                                                      18


<210>  29
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-17bp

<400>  29

ctaggaatat tgaaggg                                                       17


<210>  30
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-16bp

<400>  30

ctaggaatat tgaagg                                                        16


<210>  31
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-15bp

<400>  31

ctaggaatat tgaag                                                         15
 

<210>  32
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  VEGFa-14bp

<400>  32

ctaggaatat tgaa                                                          14


<210>  33
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-1

<400>  33

attctcaaac aaatgtgtca caa                                                23


<210>  34
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-2

<400>  34

catgtgcaaa cgccttcaac aac                                                23


<210>  35
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-3

<400>  35

cacatgcaaa gtcagatttg ttg                                                 23


<210>  36
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-4

<400>  36

ttgctccagg ccacagcact gtt                                                 23


<210>  37
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-5

<400>  37

tctgtgatat acacatcaga atc                                                 23


<210>  38
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-6

<400>  38

tgacacattt gtttgagaat caa                                                 23


<210>  39
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-7

<400>  39

tttgagaatc aaaatcggtg aat                                                 23


<210>  40
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-8

<400>  40

agaatcaaaa tcggtgaata ggc                                                 23


<210>  41
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-9

<400>  41

gagtctctca gctggtacac ggc                                                 23


<210>  42
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-1

<400>  42

agccatcaga agcagagatc tcc                                                 23


<210>  43
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-2

<400>  43

cctcggtgtc ctaccagcaa gggg                                                24


<210>  44
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-3

<400>  44

gccctatcct gggtccactc gtca                                                24


<210>  45
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TCR-4

<400>  45

ggtgtgggag atctctgctt ctga                                                24


<210>  46
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD52-1

<400>  46

tatctgtacc ataaccagga ggc                                                 23


<210>  47
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD52-2

<400>  47

tcctgagagt ccagtttgta tct                                                 23


<210>  48
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD52-3

<400>  48

gctggtgtcg ttttgtcctg aga                                                 23


<210>  49
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD52-4

<400>  49

cttttcttcg tggccaatgc cat                                                 23


<210>  50
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD52-5

<400>  50

ttcgtggcca atgccataat cca                                                 23
  

<210>  51
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  B2m-1

<400>  51

atccatccga cattgaagtt gac                                                 23


<210>  52
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  B2m-2

<400>  52
 
gctgtgctcg cgctactctc tct                                                 23


<210>  53
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-1

<400>  53

accttccgct cacctccgcc tgag                                                24


<210>  54
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-2

<400>  54

tgcccttcca gagagaaggg cag                                                 23


<210>  55
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-3

<400>  55

tctgcaggga caataggagc cag                                                 23


<210>  56
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-4

<400>  56

tcctcaaaga aggaggaccc ctc                                                  23


<210>  57
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-5

<400>  57

cagtggcgag agaagacccc gga                                                  23


<210>  58
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-6

<400>  58

ctagcggaat gggcacctca tcc                                                  23


<210>  59
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-7

<400>  59

ctcaggagaa gcaggcaggg tgc                                                  23


<210>  60
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-8

<400>  60

caacacatcg gagagcttcg tgc                                                  23


<210>  61
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PD-9

<400>  61

atctgcgcct tgggggccag gga                                                  23
