                         SEQUENCE LISTING

<110>  UCL Business PCL
 
<120>  Complex for the delivery of Cas9 proteins and guide RNA to cells

<130>  P027770WO

<150>  GB1803419.9
<151>  2018-03-02

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polycationic nucleic acid binding component

<400>  1

Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10                  15      


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavable portion of spacer element peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The residue at this position may be K or V

<400>  2

Arg Lys Lys Arg 
1               


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavable portion of spacer element peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The residue at this position may be K or V

<400>  3

Arg Lys Arg Arg 
1               


<210>  4
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavable portion of spacer element peptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The residue at this position may be F, I, L, V or Y, preferably 
       F, L or Y

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The residue at this position may be A, E or G

<400>  4

Gly Phe Phe Ala 
1               


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavable portion of spacer element peptide

<400>  5

Gly Phe Leu Gly 
1               


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker portion of spacer element peptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<400>  6

Ala Ser Ala Gly Gly 
1               5   


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker portion of spacer element peptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<400>  7

Ala Ser Ala Gly Ala 
1               5   


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  8

Arg Arg Glu Thr Ala Trp Ala 
1               5           


<210>  9
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The residue at this position is P or Q, preferably P

<400>  9

Leu Pro His Lys 
1               


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The residue at this position is A or T, preferably A

<400>  10

Pro Ser Gly Ala Ala Arg Ala 
1               5           


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The residue at this position may be any acidic amino acid 
       residue, preferably E or Q

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The residue at this position may be any acidic amino acid 
       residue, preferably E or Q, more preferably E

<400>  11

Ser Glu Arg Ser Met Asn Phe 
1               5           


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The residue at this position may be P or Q, preferably P

<400>  12

Leu Pro His Lys Ser Met Pro 
1               5           


<210>  13
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  13

Pro Ser Gly Ala Ala Arg Ala 
1               5           


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  14

Leu Gln His Lys Ser Met Pro 
1               5           


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  15

Leu Pro His Lys Ser Met Pro 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  16

Val Lys Ser Met Val Thr His 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  17

Ser Glu Arg Ser Met Asn Phe 
1               5           


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  18

Val Gly Leu Pro His Lys Phe 
1               5           


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  19

Tyr Gly Leu Pro His Lys Phe 
1               5           


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  20

Pro Ser Gly Ala Ala Arg Ala 
1               5           


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  21

Ser Gln Arg Ser Met Asn Phe 
1               5           


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  22

Pro Ser Gly Thr Ala Arg Ala 
1               5           


<210>  23
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<400>  23

Pro Ala Ala Ala Thr 
1               5   


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<400>  24

Pro Ser Ala Ser 
1               


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  The residue at this position may be any amino acid with an amide 
       side chain, such as N or Q

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  The residue at this position can be any naturally occuring amino 
       acid

<400>  25

Gln Ala Asn Ala Gln 
1               5   


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  26

Cys Arg Gly Asp Cys Leu Gly 
1               5           


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  27

Cys Arg Gly Asp Cys Leu Gly 
1               5           


<210>  28
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  28

Ala Cys Asp Cys Arg Gly Asp Cys Phe Cys Gly 
1               5                   10      


<210>  29
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  29

Cys Arg Gly Asp Met Phe Gly Cys Ala 
1               5                   


<210>  30
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  30

Cys Arg Arg Glu Thr Ala Trp Ala Cys Gly 
1               5                   10  


<210>  31
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  31

Cys Arg Gly Glu Met Phe Gly Cys Ala 
1               5                   


<210>  32
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  32

Cys Ser Glu Arg Ser Met Asn Phe Cys Gly 
1               5                   10  


<210>  33
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  33

Cys Tyr Gly Leu Pro His Lys Phe Cys Gly 
1               5                   10  


<210>  34
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cell surface receptor binding component

<400>  34

Cys Leu Pro His Lys Ser Met Pro Cys Gly 
1               5                   10  


<210>  35
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transfection vector

<400>  35

Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10                  15      


Gly Ala Cys Tyr Gly Leu Pro His Lys Phe Cys Gly 
            20                  25              


<210>  36
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transfection vector

<400>  36

Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys 
1               5                   10                  15      


Gly Ala Cys Ser Glu Arg Ser Met Asn Phe Cys Gly 
            20                  25              


<210>  37
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavable portion of spacer element peptide

<400>  37

Arg Val Arg Arg 
1               


