                         SEQUENCE LISTING

<110>  Biotangents Limited
 
<120>  MEANS AND METHODS FOR NUCLEIC ACID TARGET DETECTION

<130>  P248851WO

<150>  GB1803367.0
<151>  2018-03-01

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  163
<212>  RNA
<213>  Cyanophage N1

<400>  1
ggguucuaca uugauguugg caaucuucca aagguaaaag cagaacaaua ccucagagag       60

guaaugggac guuaccgcaa caaacuuguu uaugaugcaa acacagguga aaucaaggac      120

gacaagaaac auaugucgau gcuugcuagu uauugcucag cgg                        163


<210>  2
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  template probe


<220>
<221>  misc_feature
<222>  (69)..(69)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (96)..(96)
<223>  hexaethylenglycol (HEG) moiety

<400>  2
tcgtcttccg gtctctcctc tcaagcctca gcgctctctc tccctatagt gagtcgtatt       60

aatttcgaaa cgtcccatta cctctctgag gtattg                                 96


<210>  3
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amplification probe


<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (117)..(117)
<223>  3' phosphorylation

<400>  3
tagtataagt aaatcgcttg ctgtatgtcg ttattctgcc gtagggcacc ctatagtgag       60

tcgtattaat ttctcgtctt ccggtctctc ctctcaagcc tcagcgctct ctctccc         117


<210>  4
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Signal RNA

<400>  4
gggcaugaau aacgacauac agcaagcgau uuacuuauac ua                          42


<210>  5
<211>  92
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Toehold switch B

<400>  5
gggagaaagu aaaucgcuug cuguaugucg uuaaacagag gagauaacga augacagcaa       60

gcaaccuggc ggcagcgcaa aagaugcgua aa                                     92


<210>  6
<211>  3075
<212>  DNA
<213>  Escherichia coli

<400>  6
atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct       60

ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc      120

gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc      180

tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct      240

gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc      300

tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg      360

acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg      420

cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc      480

ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc      540

ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat      600

caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact      660

acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta      720

ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct      780

ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc      840

gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa      900

ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac      960

ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat     1020

ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat     1080

catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg     1140

aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac     1200

acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc     1260

atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc     1320

gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg     1380

aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat     1440

ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt     1500

tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc     1560

atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc     1620

cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat     1680

ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat     1740

gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc     1800

cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa     1860

gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc     1920

agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat     1980

ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg     2040

attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc     2100

gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag     2160

tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat     2220

ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac     2280

cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg     2340

ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc     2400

cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa     2460

gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct     2520

cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat     2580

ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg     2640

gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga     2700

ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat     2760

ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc     2820

gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc     2880

agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa     2940

gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg     3000

agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc     3060

tggtgtcaaa aataa                                                      3075


<210>  7
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RT-qPCR Forward Primer

<400>  7
ggtgccctac ggcag                                                        15


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RT-qPCR Reverse Primer

<400>  8
tagtataagt aaatcgcttg ctgtatgtcg                                        30


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Forward Primer (S19)

<400>  9
gaagtctaac gctgctctgg                                                   20


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Reverse Primer (S20)

<400>  10
tctcaaatgc ctgaggtttc ag                                                22


<210>  11
<211>  20
<212>  DNA
<213>  Bacteriophage T7

<400>  11
taatacgact cactataggg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Bacteriophage T3

<400>  12
aattaaccct cactaaaggg                                                   20


<210>  13
<211>  21
<212>  DNA
<213>  Bacteriophage SP6

<400>  13
aatttaggtg acactataga a                                                 21


<210>  14
<211>  58
<212>  DNA
<213>  Bacteriophage T5

<400>  14
tcataaaaaa tttatttgct ttcaggaaaa tttttctgta taatagattc ataaattt         58


<210>  15
<211>  12
<212>  DNA
<213>  Bacteriophage T7

<400>  15
atcgtcagtc cc                                                           12


<210>  16
<211>  12
<212>  DNA
<213>  Bacteriophage T7

<400>  16
gctctctctc cc                                                           12


<210>  17
<211>  12
<212>  DNA
<213>  Bacteriophage T7

<400>  17
atcctctctc cc                                                           12


<210>  18
<211>  12
<212>  DNA
<213>  Bacteriophage T7

<400>  18
gttctctctc cc                                                           12


<210>  19
<211>  8
<212>  DNA
<213>  Bacteriophage T7

<400>  19
agaggaga                                                                 8


<210>  20
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extension probe

<400>  20
gtttgcatca taaacaagtt tgttgcggta ttcgaaat                               38


<210>  21
<211>  60
<212>  RNA
<213>  Bovine viral diarrhoea virus

<400>  21
auggaguuga ucacaaauga acuuuuauac aaaacauaca aacaaaaacc cgcuggagug       60


<210>  22
<211>  75
<212>  RNA
<213>  Mycoplasma mycoides

<400>  22
ugaugaaaaa auugacaagc caagucauuc agauaaacca caagcagaug auucuaacaa       60

caauagagac auuuu                                                        75


<210>  23
<211>  57
<212>  RNA
<213>  Mycoplasma capricolum

<400>  23
cacaauucgg aguuucacua gauaaaguug augcuacauu uuuaacaucu ccucagc          57


<210>  24
<211>  327
<212>  RNA
<213>  Bos taurus

<400>  24
gggauccugc caacaucaag uggggugaug cuggugcuga guauguggug gaguccacug       60

gggucuucac uaccauggag aaggcugggg cucacuugaa ggguggcgcc aagaggguca      120

ucaucucugc accuucugcc gaugccccca uguuugugau gggcgugaac cacgagaagu      180

auaacaacac ccucaagauu gucagcaaug ccuccugcac caccaacugc uuggcccccc      240

uggccaaggu cauccaugac cacuuuggca ucguggaggg acuuaugacc acuguccacg      300

ccaucacugc uaguuauugc ucagcgg                                          327


<210>  25
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BVDV Extension probe

<400>  25
cactccagcg ggtttttgtt tgtatgttct agattatg                               38


<210>  26
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BVDV Template probe (producing signal RND1 RNA)


<220>
<221>  misc_feature
<222>  (77)..(77)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (113)..(113)
<223>  3' phosphorylation

<400>  26
atctgtttcc gtcatcctta gtccattccc atcatcgtcc agttctctct ccctatagtg       60

agtcgtatta cataatctgt ataaaagttc atttgtgatc aactccattt ttt             113


<210>  27
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BVDV Template probe (producing signal B RNA)


<220>
<221>  misc_feature
<222>  (75)..(75)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (106)..(106)
<223>  3' phosphorylation

<400>  27
tagtataagt aaatcgcttg ctgtatgtcg ttattctgcc gtagggcacc ctatagtgag       60

tcgtattaca taatctgtat aaaagttcat ttgtgatcaa ctccat                     106


<210>  28
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BVDV Template probe (producing signal 42_23 RNA)


<220>
<221>  misc_feature
<222>  (77)..(77)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (108)..(108)
<223>  3' phosphorylation

<400>  28
ctggaactgg atggatgtca ttgcgtaaag cctctatgca ccttatggtg ccctatagtg       60

agtcgtatta cataatctgt ataaaagttc atttgtgatc aactccat                   108


<210>  29
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CBPP Extension Probe

<400>  29
gtctctattg ttgttagaat catctgcttg tgacggatta tg                          42


<210>  30
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CBPP Template Probe


<220>
<221>  misc_feature
<222>  (77)..(77)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (110)..(110)
<223>  3' phosphorylation

<400>  30
atctgtttcc gtcatcctta gtccattccc atcatcgtcc agttctctct ccctatagtg       60

agtcgtatta cataatcgtt tatctgaatg acttggcttg tcaatttttt                 110


<210>  31
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CCPP Extension probe

<400>  31
ggagatgtta aaaatgtagc atcagttgat tatg                                   34


<210>  32
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CCPP Template probe


<220>
<221>  misc_feature
<222>  (77)..(77)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (106)..(106)
<223>  3' phosphorylation

<400>  32
atctgtttcc gtcatcctta gtccattccc atcatcgtcc agttctctct ccctatagtg       60

agtcgtatta cataatcctt tatctagtga aactccgaat tgtgaa                     106


<210>  33
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH Extension probe

<400>  33
gttggtggtg caggaggcat tgctgacaat actgattatg                             40


<210>  34
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH Template probe


<220>
<221>  misc_feature
<222>  (77)..(77)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (113)..(113)
<223>  3' phosphorylation

<400>  34
atctgtttcc gtcatcctta gtccattccc atcatcgtcc agttctctct ccctatagtg       60

agtcgtatta cataatcctt gagggtgttg ttatacttct cgtggttttt ttt             113


<210>  35
<211>  53
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Signal RNA (signal RND1)

<400>  35
gggagagaga acuggacgau gaugggaaug gacuaaggau gacggaaaca gau              53


<210>  36
<211>  51
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Signal RNA (signal B)

<400>  36
gggugcccua cggcagaaua acgacauaca gcaagcgauu uacuuauacu a                51


<210>  37
<211>  53
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Signal RNA (signal 42_23)

<400>  37
gggcaccaua aggugcauag aggcuuuacg caaugacauc cauccaguuc cag              53


<210>  38
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Signal DNA (signal RND1)

<400>  38
gggagagaga actggacgat gatgggaatg gactaaggat gacggaaaca gat              53


<210>  39
<211>  112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Probe P24


<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (112)..(112)
<223>  3' phosphorylation

<400>  39
tagtataagt aaatcgcttg ctgtatgtcg ttattctgcc gtagggcacc ctatagtgag       60

tcgtattaat ttcatctgtt tccgtcatcc ttagtccatt cccatcatcg tc              112


<210>  40
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplification Probe P80


<220>
<221>  misc_feature
<222>  (84)..(84)
<223>  hexaethylenglycol (HEG) moiety

<220>
<221>  misc_feature
<222>  (114)..(114)
<223>  3' phosphorylation

<400>  40
ctggaactgg atggatgtca ttgcgtaaag cctctatgca ccttatggtg ccctatagtg       60

agtcgtatta atttcatctg tttccgtcat ccttagtcca ttcccatcat cgtc            114


<210>  41
<211>  90
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Toehold switch 117

<400>  41
gggccuuagu ccauucccau caucguccaa ggccucuaga caaugaaaca gaggagaugg       60

acgaugauaa accuggcggc agcgcaaaag                                        90


<210>  42
<211>  92
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Toehold switch 119

<400>  42
gggccuuagu ccauucccau caucguccag uuccucuaac gcccaauaac uagaggagac       60

ggacgaugau aaaccuggcg gcagcgcaaa ag                                     92


<210>  43
<211>  94
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Toehold switch 121

<400>  43
gggccuuagu ccauucccau caucguccag uuccucuaac augccgcuaa acuagaggag       60

acggacgaug auaaaccugg cggcagcgca aaag                                   94


<210>  44
<211>  92
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Toehold switch B version 2

<400>  44
ggguauaagu aaaucgcuug cuguaugucg uuaaacagag gagauaacga augacagcaa       60

gcaaccuggc ggcagcgcaa aagaugcgua aa                                     92


<210>  45
<211>  113
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Toehold switch 42_23

<400>  45
gggagauaug aacuggaugg augucauugc guaaagccuc uauaccgaac gaaacauaga       60

ggagauacgc aaugaaacga uacaaccugg cggcagcgca aaagcaaagu aag             113


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RND1 RT-qPCR Forward Primer

<400>  46
gggagagaga actggacgat ga                                                22


<210>  47
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RND1 RT-qPCR Reverse Primer

<400>  47
atctgtttcc gtcatcctta gtccattc                                          28


<210>  48
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  42_23 RT-qPCR Forward Primer

<400>  48
caccataagg tgcatagagg ctt                                               23


<210>  49
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  42_23 RT-qPCR Reverse Primer

<400>  49
ctggaactgg atggatgtca ttgc                                              24


