                         SEQUENCE LISTING

<110>  F.Hoffmann-La Roche
 
<120>  Universal reporter cell assay for specificity test of novel 
       antigen binding moieties

<130>  P34622

<160>  123   

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG CDR H1 Kabat

<400>  1

Asp Tyr Ala Met Ser 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG CDR H2 Kabat

<400>  2

Ser Ile Asn Ile Gly Ala Thr Tyr Ile Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG CDR H3 Kabat

<400>  3

Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met Ala Tyr 
1               5                   10                  15      


<210>  4
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG CDR L1 Kabat

<400>  4

Arg Ala Ser Gln Asp Ile Lys Asn Tyr Leu Asn 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG CDR L2 Kabat

<400>  5

Tyr Ser Ser Thr Leu Leu Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG CDR L3 Kabat

<400>  6

Gln Gln Ser Ile Thr Leu Pro Pro Thr 
1               5                   


<210>  7
<211>  437
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG-ds-scFv-CD28ATD-CD28CSD-CD3zSSD fusion

<400>  7

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Ala Met Ser Trp Ile Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Asn Ile Gly Ala Thr Tyr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
145                 150                 155                 160 


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Lys Asn 
                165                 170                 175     


Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
            180                 185                 190         


Ile Tyr Tyr Ser Ser Thr Leu Leu Ser Gly Val Pro Ser Arg Phe Ser 
        195                 200                 205             


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
    210                 215                 220                 


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Ile Thr Leu Pro 
225                 230                 235                 240 


Pro Thr Phe Gly Cys Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly 
                245                 250                 255     


Ser Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
            260                 265                 270         


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg 
        275                 280                 285             


Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
    290                 295                 300                 


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
305                 310                 315                 320 


Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
                325                 330                 335     


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
            340                 345                 350         


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
        355                 360                 365             


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
    370                 375                 380                 


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
385                 390                 395                 400 


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
                405                 410                 415     


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
            420                 425                 430         


Ala Leu Pro Pro Arg 
        435         


<210>  8
<211>  125
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG-ds VH

<400>  8

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Ala Met Ser Trp Ile Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Asn Ile Gly Ala Thr Tyr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 125 


<210>  9
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG-ds VL

<400>  9

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Lys Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ser Ser Thr Leu Leu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Ile Thr Leu Pro Pro 
                85                  90                  95      


Thr Phe Gly Cys Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  10
<211>  252
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG-ds-scFv

<400>  10

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Ala Met Ser Trp Ile Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Asn Ile Gly Ala Thr Tyr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
145                 150                 155                 160 


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Lys Asn 
                165                 170                 175     


Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
            180                 185                 190         


Ile Tyr Tyr Ser Ser Thr Leu Leu Ser Gly Val Pro Ser Arg Phe Ser 
        195                 200                 205             


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
    210                 215                 220                 


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Ile Thr Leu Pro 
225                 230                 235                 240 


Pro Thr Phe Gly Cys Gly Thr Lys Val Glu Ile Lys 
                245                 250         


<210>  11
<211>  27
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CD28ATD

<400>  11

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  12
<211>  41
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CD28CSD

<400>  12

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  13
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CD3zSSD

<400>  13

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  14
<211>  180
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CD28ATD-CD28CSD-CD3zSSD

<400>  14

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  


Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
65                  70                  75                  80  


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
                85                  90                  95      


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
            100                 105                 110         


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        115                 120                 125             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    130                 135                 140                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
145                 150                 155                 160 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                165                 170                 175     


Leu Pro Pro Arg 
            180 


<210>  15
<211>  238
<212>  PRT
<213>  Artificial sequence

<220>
<223>  eGFP

<400>  15

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235             


<210>  16
<211>  20
<212>  PRT
<213>  Artificial sequence

<220>
<223>  (G4S)4 linker

<400>  16

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  17
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  G4S linker

<400>  17

Gly Gly Gly Gly Ser 
1               5   


<210>  18
<211>  19
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T2A linker

<400>  18

Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  19
<211>  1245
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Anti-DIG-ds-scFv-CD28ATD-CD28CSD-CD3zSSD fusion

<400>  19
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattcccag       60

gtgcagctcg tggagtcagg gggaggcctg gtcaagcctg gcggctccct gagactgtct      120

tgcgccgcct ctggcttcac attctccgac tacgccatga gctggatcag acaggctccc      180

ggcaaatgcc tcgagtgggt gtccagcatc aacatcggcg ccacctacat ctactatgcc      240

gactccgtga agggccggtt caccatctcc agagacaacg ccaagaatag cctctatctc      300

cagatgaact ccctgcgggc cgaagatacc gctgtgtatt actgcgccag acccggcagc      360

ccctacgagt acgacaaggc ctactacagc atggcctact ggggccaggg caccaccgtg      420

acagtgtcat ctggaggggg cggaagtggt ggcgggggaa gcggcggggg tggcagcgga      480

gggggcggat ctgacatcca gatgacccag tccccaagca gcctgagcgc cagcgtgggc      540

gacagagtga ccatcacctg tcgggccagc caggacatca agaactacct gaattggtat      600

cagcagaaac ctggcaaagc ccctaagctg ctcatctact acagctccac cctgctgagc      660

ggcgtgccca gcagattttc cggcagcggg agcggcacag atttcacact gacaatctcc      720

agcctgcagc ctgaggactt cgccacctac tattgtcagc agagcatcac cctgcccccc      780

acctttggct gtggcacaaa agtcgagatc aagggagggg gcggatcctt ctgggtgctg      840

gtggtggtgg gcggcgtgct ggcctgctac agcctgctgg tgaccgtggc cttcatcatc      900

ttctgggtga gggtgaagtt cagcaggagc gccgacgccc ccgcctacca gcagggccag      960

aaccagctgt ataacgagct gaacctgggc aggagggagg agtacgacgt gctggacaag     1020

aggaggggca gggaccccga gatgggcggc aagcccagga ggaagaaccc ccaggagggc     1080

ctgtataacg agctgcagaa ggacaagatg gccgaggcct acagcgagat cggcatgaag     1140

ggcgagagga ggaggggcaa gggccacgac ggcctgtacc agggcctgag caccgccacc     1200

aaggacacct acgacgccct gcacatgcag gccctgcccc ccagg                     1245


<210>  20
<211>  374
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Anti-DIG-ds VH

<400>  20
aggtgcagct cgtggagtca gggggaggcc tggtcaagcc tggcggctcc ctgagactgt       60

cttgcgccgc ctctggcttc acattctccg actacgccat gagctggatc agacaggctc      120

ccggcaaatg cctcgagtgg gtgtccagca tcaacatcgg cgccacctac atctactatg      180

ccgactccgt gaagggccgg ttcaccatct ccagagacaa cgccaagaat agcctctatc      240

tccagatgaa ctccctgcgg gccgaagata ccgctgtgta ttactgcgcc agacccggca      300

gcccctacga gtacgacaag gcctactaca gcatggccta ctggggccag ggcaccaccg      360

tgacagtgtc atct                                                        374


<210>  21
<211>  321
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Anti-DIG-ds VL

<400>  21
gacatccaga tgacccagtc cccaagcagc ctgagcgcca gcgtgggcga cagagtgacc       60

atcacctgtc gggccagcca ggacatcaag aactacctga attggtatca gcagaaacct      120

ggcaaagccc ctaagctgct catctactac agctccaccc tgctgagcgg cgtgcccagc      180

agattttccg gcagcgggag cggcacagat ttcacactga caatctccag cctgcagcct      240

gaggacttcg ccacctacta ttgtcagcag agcatcaccc tgccccccac ctttggctgt      300

ggcacaaaag tcgagatcaa g                                                321


<210>  22
<211>  813
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Anti-DIG-ds-scFv

<400>  22
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattcccag       60

gtgcagctcg tggagtcagg gggaggcctg gtcaagcctg gcggctccct gagactgtct      120

tgcgccgcct ctggcttcac attctccgac tacgccatga gctggatcag acaggctccc      180

ggcaaatgcc tcgagtgggt gtccagcatc aacatcggcg ccacctacat ctactatgcc      240

gactccgtga agggccggtt caccatctcc agagacaacg ccaagaatag cctctatctc      300

cagatgaact ccctgcgggc cgaagatacc gctgtgtatt actgcgccag acccggcagc      360

ccctacgagt acgacaaggc ctactacagc atggcctact ggggccaggg caccaccgtg      420

acagtgtcat ctggaggggg cggaagtggt ggcgggggaa gcggcggggg tggcagcgga      480

gggggcggat ctgacatcca gatgacccag tccccaagca gcctgagcgc cagcgtgggc      540

gacagagtga ccatcacctg tcgggccagc caggacatca agaactacct gaattggtat      600

cagcagaaac ctggcaaagc ccctaagctg ctcatctact acagctccac cctgctgagc      660

ggcgtgccca gcagattttc cggcagcggg agcggcacag atttcacact gacaatctcc      720

agcctgcagc ctgaggactt cgccacctac tattgtcagc agagcatcac cctgcccccc      780

acctttggct gtggcacaaa agtcgagatc aag                                   813


<210>  23
<211>  717
<212>  DNA
<213>  Artificial sequence

<220>
<223>  eGFP

<400>  23
gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc       60

gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc      120

aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc      180

gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag      240

cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc      300

aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg      360

aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag      420

ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc      480

atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac      540

cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac      600

ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg      660

ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtga         717


<210>  24
<211>  81
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD28ATD

<400>  24
ttttgggtgc tggtggtggt tggtggagtc ctggcttgct atagcttgct agtaacagtg       60

gcctttatta ttttctgggt g                                                 81


<210>  25
<211>  123
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD28CSD

<400>  25
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210>  26
<211>  336
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD3zSSD

<400>  26
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210>  27
<211>  540
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CD28ATD-CD28CSD-CD3zSSD

<400>  27
ttctgggtgc tggtggtggt gggcggcgtg ctggcctgct acagcctgct ggtgaccgtg       60

gccttcatca tcttctgggt gaggagcaag aggagcaggc tgctgcacag cgactacatg      120

aacatgaccc ccaggaggcc cggccccacc aggaagcact accagcccta cgcccccccc      180

agggacttcg ccgcctacag gagcagggtg aagttcagca ggagcgccga cgcccccgcc      240

taccagcagg gccagaacca gctgtataac gagctgaacc tgggcaggag ggaggagtac      300

gacgtgctgg acaagaggag gggcagggac cccgagatgg gcggcaagcc caggaggaag      360

aacccccagg agggcctgta taacgagctg cagaaggaca agatggccga ggcctacagc      420

gagatcggca tgaagggcga gaggaggagg ggcaagggcc acgacggcct gtaccagggc      480

ctgagcaccg ccaccaagga cacctacgac gccctgcaca tgcaggccct gccccccagg      540


<210>  28
<211>  63
<212>  DNA
<213>  Artificial sequence

<220>
<223>  T2A element

<400>  28
tccggagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct       60

agg                                                                     63


<210>  29
<211>  413
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG-Fab-heavy chain- CD28ATD-CD28CSD-CD3zSSD fusion 
       pETR17594

<400>  29

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Ala Met Ser Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Asn Ile Gly Ala Thr Tyr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr 
        115                 120                 125             


Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
    130                 135                 140                 


Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
145                 150                 155                 160 


Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
                165                 170                 175     


Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
            180                 185                 190         


Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
        195                 200                 205             


Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu 
    210                 215                 220                 


Pro Lys Ser Cys Gly Gly Gly Gly Ser Phe Trp Val Leu Val Val Val 
225                 230                 235                 240 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                245                 250                 255     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            260                 265                 270         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        275                 280                 285             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys 
    290                 295                 300                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
305                 310                 315                 320 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                325                 330                 335     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            340                 345                 350         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        355                 360                 365             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    370                 375                 380                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
385                 390                 395                 400 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                405                 410             


<210>  30
<211>  228
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG-Fab heavy chain

<400>  30

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Ala Met Ser Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Asn Ile Gly Ala Thr Tyr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ser Thr 
        115                 120                 125             


Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
    130                 135                 140                 


Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
145                 150                 155                 160 


Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
                165                 170                 175     


Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
            180                 185                 190         


Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
        195                 200                 205             


Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu 
    210                 215                 220                 


Pro Lys Ser Cys 
225             


<210>  31
<211>  214
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG-Fab light chain

<400>  31

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Lys Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ser Ser Thr Leu Leu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Ile Thr Leu Pro Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  32
<211>  125
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG VH

<400>  32

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Ala Met Ser Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Asn Ile Gly Ala Thr Tyr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 125 


<210>  33
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-DIG VL

<400>  33

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Lys Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ser Ser Thr Leu Leu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Ile Thr Leu Pro Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  34
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CL

<400>  34

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  35
<211>  103
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CH1 (human)

<400>  35

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys 
            100             


<210>  36
<211>  3319
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Anti-DIG-Fab-heavy chain- CD28ATD-CD28CSD-CD3zSSD fusion 
       pETR17176

<400>  36
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcactccgat       60

attcagatga cccagagccc gagcagcctg agcgcgagcg tgggcgatcg cgtgaccatt      120

acctgccgcg cgagccagga tattaaaaac tatctgaact ggtatcagca gaaaccgggc      180

aaagcgccga aactgctgat ttattatagc agcaccctgc tgagcggcgt gccgagccgc      240

tttagcggca gcggcagcgg caccgatttt accctgacca ttagcagcct gcagccggaa      300

gattttgcga cctattattg ccagcagagc attaccctgc cgccgacctt tggcggcggc      360

accaaagtgg aaattaaacg cactgtcgcc gctccctctg tgttcatttt tcctccaagt      420

gatgagcagc tcaaaagcgg taccgcatcc gttgtgtgcc tgcttaacaa cttctatccc      480

cgggaagcca aggtccaatg gaaggtggac aatgctctgc agtcaggaaa cagtcaggag      540

agcgtaaccg agcaggattc caaagactct acttactcat tgagctccac cctgacactc      600

tctaaggcag actatgaaaa gcataaagtg tacgcctgtg aggttaccca ccagggcctg      660

agtagccctg tgacaaagtc cttcaatagg ggagagtgct agaatagaat tccccgaagt      720

aacttagaag ctgtaaatca acgatcaata gcaggtgtgg cacaccagtc ataccttgat      780

caagcacttc tgtttccccg gactgagtat caataggctg ctcgcgcggc tgaaggagaa      840

aacgttcgtt acccgaccaa ctacttcgag aagcttagta ccaccatgaa cgaggcaggg      900

tgtttcgctc agcacaaccc cagtgtagat caggctgatg agtcactgca acccccatgg      960

gcgaccatgg cagtggctgc gttggcggcc tgcccatgga gaaatccatg ggacgctcta     1020

attctgacat ggtgtgaagt gcctattgag ctaactggta gtcctccggc ccctgattgc     1080

ggctaatcct aactgcggag cacatgctca caaaccagtg ggtggtgtgt cgtaacgggc     1140

aactctgcag cggaaccgac tactttgggt gtccgtgttt ccttttattc ctatattggc     1200

tgcttatggt gacaatcaaa aagttgttac catatagcta ttggattggc catccggtgt     1260

gcaacagggc aactgtttac ctatttattg gttttgtacc attatcactg aagtctgtga     1320

tcactctcaa attcattttg accctcaaca caatcaaacg ccaccatggg atggagctgt     1380

atcatcctct tcttggtagc aacagctact ggtgtgcatt cccaggtgca gctggtggaa     1440

agcggcggcg gcctggtgaa accgggcggc agcctgcgcc tgagctgcgc ggcgagcggc     1500

tttaccttta gcgattatgc gatgagctgg attcgccagg cgccgggcaa aggcctggaa     1560

tgggtgagca gcattaacat tggcgcgacc tatatttatt atgcggatag cgtgaaaggc     1620

cgctttacca ttagccgcga taacgcgaaa aacagcctgt atctgcagat gaacagcctg     1680

cgcgcggaag ataccgcggt gtattattgc gcgcgcccgg gcagcccgta tgaatatgat     1740

aaagcgtatt atagcatggc gtattggggc cagggcacca ccgtgaccgt gagcagcgcg     1800

tcgactaagg gcccttcagt ttttccactc gcccccagta gcaagtccac atctgggggt     1860

accgctgccc tgggctgcct tgtgaaagac tatttccctg aaccagtcac tgtgtcatgg     1920

aatagcggag ccctgacctc cggtgtacac acattccccg ctgtgttgca gtctagtggc     1980

ctgtacagcc tctcctctgt tgtgaccgtc ccttcaagct ccctggggac acagacctat     2040

atctgtaacg tgaatcataa gccatctaac actaaagtag ataaaaaagt ggagcccaag     2100

agttgcggag ggggcggatc cttctgggtg ctggtggtgg tgggcggcgt gctggcctgc     2160

tacagcctgc tggtgaccgt ggccttcatc atcttctggg tgagggtgaa gttcagcagg     2220

agcgccgacg cccccgccta ccagcagggc cagaaccagc tgtataacga gctgaacctg     2280

ggcaggaggg aggagtacga cgtgctggac aagaggaggg gcagggaccc cgagatgggc     2340

ggcaagccca ggaggaagaa cccccaggag ggcctgtata acgagctgca gaaggacaag     2400

atggccgagg cctacagcga gatcggcatg aagggcgaga ggaggagggg caagggccac     2460

gacggcctgt accagggcct gagcaccgcc accaaggaca cctacgacgc cctgcacatg     2520

caggccctgc cccccaggtc cggagagggc agaggaagtc ttctaacatg cggtgacgtg     2580

gaggagaatc ccggccctag ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc     2640

atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc     2700

gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg     2760

cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc     2820

taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc     2880

caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag     2940

ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac     3000

ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg     3060

gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac     3120

ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg     3180

ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag     3240

aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg     3300

gacgagctgt acaagtgat                                                  3319


<210>  37
<211>  321
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Anti-DIG VL

<400>  37
gatattcaga tgacccagag cccgagcagc ctgagcgcga gcgtgggcga tcgcgtgacc       60

attacctgcc gcgcgagcca ggatattaaa aactatctga actggtatca gcagaaaccg      120

ggcaaagcgc cgaaactgct gatttattat agcagcaccc tgctgagcgg cgtgccgagc      180

cgctttagcg gcagcggcag cggcaccgat tttaccctga ccattagcag cctgcagccg      240

gaagattttg cgacctatta ttgccagcag agcattaccc tgccgccgac ctttggcggc      300

ggcaccaaag tggaaattaa a                                                321


<210>  38
<211>  321
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CL

<400>  38
cgcactgtcg ccgctccctc tgtgttcatt tttcctccaa gtgatgagca gctcaaaagc       60

ggtaccgcat ccgttgtgtg cctgcttaac aacttctatc cccgggaagc caaggtccaa      120

tggaaggtgg acaatgctct gcagtcagga aacagtcagg agagcgtaac cgagcaggat      180

tccaaagact ctacttactc attgagctcc accctgacac tctctaaggc agactatgaa      240

aagcataaag tgtacgcctg tgaggttacc caccagggcc tgagtagccc tgtgacaaag      300

tccttcaata ggggagagtg c                                                321


<210>  39
<211>  375
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Anti-DIG VH

<400>  39
caggtgcagc tggtggaaag cggcggcggc ctggtgaaac cgggcggcag cctgcgcctg       60

agctgcgcgg cgagcggctt tacctttagc gattatgcga tgagctggat tcgccaggcg      120

ccgggcaaag gcctggaatg ggtgagcagc attaacattg gcgcgaccta tatttattat      180

gcggatagcg tgaaaggccg ctttaccatt agccgcgata acgcgaaaaa cagcctgtat      240

ctgcagatga acagcctgcg cgcggaagat accgcggtgt attattgcgc gcgcccgggc      300

agcccgtatg aatatgataa agcgtattat agcatggcgt attggggcca gggcaccacc      360

gtgaccgtga gcagc                                                       375


<210>  40
<211>  309
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CH1

<400>  40
gcgtcgacta agggcccttc agtttttcca ctcgccccca gtagcaagtc cacatctggg       60

ggtaccgctg ccctgggctg ccttgtgaaa gactatttcc ctgaaccagt cactgtgtca      120

tggaatagcg gagccctgac ctccggtgta cacacattcc ccgctgtgtt gcagtctagt      180

ggcctgtaca gcctctcctc tgttgtgacc gtcccttcaa gctccctggg gacacagacc      240

tatatctgta acgtgaatca taagccatct aacactaaag tagataaaaa agtggagccc      300

aagagttgc                                                              309


<210>  41
<211>  647
<212>  DNA
<213>  Artificial sequence

<220>
<223>  IRES EV71, internal ribosomal entry side

<400>  41
cccgaagtaa cttagaagct gtaaatcaac gatcaatagc aggtgtggca caccagtcat       60

accttgatca agcacttctg tttccccgga ctgagtatca ataggctgct cgcgcggctg      120

aaggagaaaa cgttcgttac ccgaccaact acttcgagaa gcttagtacc accatgaacg      180

aggcagggtg tttcgctcag cacaacccca gtgtagatca ggctgatgag tcactgcaac      240

ccccatgggc gaccatggca gtggctgcgt tggcggcctg cccatggaga aatccatggg      300

acgctctaat tctgacatgg tgtgaagtgc ctattgagct aactggtagt cctccggccc      360

ctgattgcgg ctaatcctaa ctgcggagca catgctcaca aaccagtggg tggtgtgtcg      420

taacgggcaa ctctgcagcg gaaccgacta ctttgggtgt ccgtgtttcc ttttattcct      480

atattggctg cttatggtga caatcaaaaa gttgttacca tatagctatt ggattggcca      540

tccggtgtgc aacagggcaa ctgtttacct atttattggt tttgtaccat tatcactgaa      600

gtctgtgatc actctcaaat tcattttgac cctcaacaca atcaaac                    647


<210>  42
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC CDR H1 Kabat

<400>  42

His Tyr Trp Met Asn 
1               5   


<210>  43
<211>  19
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti- FITC  CDR H2 Kabat

<400>  43

Gln Phe Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp Ser 
1               5                   10                  15      


Val Lys Gly 
            


<210>  44
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC CDR H3 Kabat

<400>  44

Ala Ser Tyr Gly Met Glu Tyr 
1               5           


<210>  45
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC CDR L1 Kabat

<400>  45

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu Arg 
1               5                   10                  15      


<210>  46
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC CDR L2 Kabat

<400>  46

Lys Val Ser Asn Arg Val Ser 
1               5           


<210>  47
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC CDR L3 Kabat

<400>  47

Ser Gln Ser Thr His Val Pro Trp Thr 
1               5                   


<210>  48
<211>  436
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC-scFv-CD28ATD-CD28CSD-CD3zSSD fusion

<400>  48

Gly Val Lys Leu Asp Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ala Met Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Gly His Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Gln Phe Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Asn Leu Arg Val Glu Asp Thr Gly Ile Tyr 
                85                  90                  95      


Tyr Cys Thr Gly Ala Ser Tyr Gly Met Glu Tyr Leu Gly Gln Gly Thr 
            100                 105                 110         


Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln 
    130                 135                 140                 


Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser 
145                 150                 155                 160 


Cys Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu 
                165                 170                 175     


Arg Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr 
            180                 185                 190         


Lys Val Ser Asn Arg Val Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Asn Arg Val Glu Ala Glu 
    210                 215                 220                 


Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr 
225                 230                 235                 240 


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser 
                245                 250                 255     


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
            260                 265                 270         


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
        275                 280                 285             


Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
    290                 295                 300                 


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
305                 310                 315                 320 


Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
                325                 330                 335     


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
            340                 345                 350         


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
        355                 360                 365             


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
    370                 375                 380                 


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
385                 390                 395                 400 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
                405                 410                 415     


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
            420                 425                 430         


Leu Pro Pro Arg 
        435     


<210>  49
<211>  250
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC-scFv

<400>  49

Gly Val Lys Leu Asp Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ala Met Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Gly His Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Gln Phe Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Asn Leu Arg Val Glu Asp Thr Gly Ile Tyr 
                85                  90                  95      


Tyr Cys Thr Gly Ala Ser Tyr Gly Met Glu Tyr Leu Gly Gln Gly Thr 
            100                 105                 110         


Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln 
    130                 135                 140                 


Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser 
145                 150                 155                 160 


Cys Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu 
                165                 170                 175     


Arg Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr 
            180                 185                 190         


Lys Val Ser Asn Arg Val Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Asn Arg Val Glu Ala Glu 
    210                 215                 220                 


Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr 
225                 230                 235                 240 


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
                245                 250 


<210>  50
<211>  118
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC VH

<400>  50

Gly Val Lys Leu Asp Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ala Met Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Gly His Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Gln Phe Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Asn Leu Arg Val Glu Asp Thr Gly Ile Tyr 
                85                  90                  95      


Tyr Cys Thr Gly Ala Ser Tyr Gly Met Glu Tyr Leu Gly Gln Gly Thr 
            100                 105                 110         


Ser Val Thr Val Ser Ser 
        115             


<210>  51
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-FITC VL

<400>  51

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Arg Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Val Leu Ile Tyr Lys Val Ser Asn Arg Val Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Asn Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  52
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA CDR H1 Kabat

<400>  52

Asn Tyr Asp Met Ala 
1               5   


<210>  53
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA CDR H2 Kabat

<400>  53

Thr Ile Ser His Asp Gly Arg Asn Thr Asn Tyr Arg Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  54
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA CDR H3 Kabat

<400>  54

Pro Gly Phe Ala His 
1               5   


<210>  55
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA CDR L1 Kabat

<400>  55

Arg Ser Ser Lys Thr Leu Leu Asn Thr Arg Gly Ile Thr Ser Leu Tyr 
1               5                   10                  15      


<210>  56
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA CDR L2 Kabat

<400>  56

Arg Met Ser Asn Leu Ala Ser 
1               5           


<210>  57
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA CDR L3 Kabat

<400>  57

Ala Gln Phe Leu Glu Phe Pro Leu Thr 
1               5                   


<210>  58
<211>  431
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA-scFv-CD28ATD-CD28CSD-CD3zSSD fusion

<400>  58

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Ala Val Ser Gly Phe Ile Phe Ser Asn Tyr 
            20                  25                  30          


Asp Met Ala Trp Val Arg Gln Ala Pro Lys Lys Cys Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser His Asp Gly Arg Asn Thr Asn Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Gly Ser Arg Asp Ser Ala Gln Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Ile Tyr Phe Cys 
                85                  90                  95      


Ala Gly Pro Gly Phe Ala His Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ala Pro Leu Ser 
    130                 135                 140                 


Val Ser Val Ser Pro Gly Glu Ser Ala Ser Ile Ser Cys Arg Ser Ser 
145                 150                 155                 160 


Lys Thr Leu Leu Asn Thr Arg Gly Ile Thr Ser Leu Tyr Trp Tyr Leu 
                165                 170                 175     


Gln Lys Pro Gly Lys Ser Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn 
            180                 185                 190         


Leu Ala Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Glu Thr 
        195                 200                 205             


His Phe Thr Leu Gln Ile Ser Lys Val Glu Thr Glu Asp Val Gly Ile 
    210                 215                 220                 


Tyr Tyr Cys Ala Gln Phe Leu Glu Phe Pro Leu Thr Phe Gly Cys Gly 
225                 230                 235                 240 


Thr Lys Leu Glu Ile Lys Gly Gly Gly Gly Ser Phe Trp Val Leu Val 
                245                 250                 255     


Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala 
            260                 265                 270         


Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser 
        275                 280                 285             


Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His 
    290                 295                 300                 


Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg 
305                 310                 315                 320 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
                325                 330                 335     


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
            340                 345                 350         


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
        355                 360                 365             


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
    370                 375                 380                 


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
385                 390                 395                 400 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
                405                 410                 415     


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            420                 425                 430     


<210>  59
<211>  246
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA-scFv

<400>  59

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Ala Val Ser Gly Phe Ile Phe Ser Asn Tyr 
            20                  25                  30          


Asp Met Ala Trp Val Arg Gln Ala Pro Lys Lys Cys Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser His Asp Gly Arg Asn Thr Asn Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Gly Ser Arg Asp Ser Ala Gln Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Ile Tyr Phe Cys 
                85                  90                  95      


Ala Gly Pro Gly Phe Ala His Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ala Pro Leu Ser 
    130                 135                 140                 


Val Ser Val Ser Pro Gly Glu Ser Ala Ser Ile Ser Cys Arg Ser Ser 
145                 150                 155                 160 


Lys Thr Leu Leu Asn Thr Arg Gly Ile Thr Ser Leu Tyr Trp Tyr Leu 
                165                 170                 175     


Gln Lys Pro Gly Lys Ser Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn 
            180                 185                 190         


Leu Ala Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ser Glu Thr 
        195                 200                 205             


His Phe Thr Leu Gln Ile Ser Lys Val Glu Thr Glu Asp Val Gly Ile 
    210                 215                 220                 


Tyr Tyr Cys Ala Gln Phe Leu Glu Phe Pro Leu Thr Phe Gly Cys Gly 
225                 230                 235                 240 


Thr Lys Leu Glu Ile Lys 
                245     


<210>  60
<211>  114
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA VH

<400>  60

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Ala Val Ser Gly Phe Ile Phe Ser Asn Tyr 
            20                  25                  30          


Asp Met Ala Trp Val Arg Gln Ala Pro Lys Lys Cys Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser His Asp Gly Arg Asn Thr Asn Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Gly Ser Arg Asp Ser Ala Gln Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Ile Tyr Phe Cys 
                85                  90                  95      


Ala Gly Pro Gly Phe Ala His Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  61
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA VL

<400>  61

Asp Ile Val Leu Thr Gln Ala Pro Leu Ser Val Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Ser Ala Ser Ile Ser Cys Arg Ser Ser Lys Thr Leu Leu Asn Thr 
            20                  25                  30          


Arg Gly Ile Thr Ser Leu Tyr Trp Tyr Leu Gln Lys Pro Gly Lys Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Ile Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Glu Thr His Phe Thr Leu Gln Ile 
65                  70                  75                  80  


Ser Lys Val Glu Thr Glu Asp Val Gly Ile Tyr Tyr Cys Ala Gln Phe 
                85                  90                  95      


Leu Glu Phe Pro Leu Thr Phe Gly Cys Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  62
<211>  402
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA-Fab-heavy chain- CD28ATD-CD28CSD-CD3zSSD fusion

<400>  62

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Ala Val Ser Gly Phe Ile Phe Ser Asn Tyr 
            20                  25                  30          


Asp Met Ala Trp Val Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser His Asp Gly Arg Asn Thr Asn Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Gly Ser Arg Asp Ser Ala Gln Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Ile Tyr Phe Cys 
                85                  90                  95      


Ala Gly Pro Gly Phe Ala His Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser 
        115                 120                 125             


Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys 
    130                 135                 140                 


Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
145                 150                 155                 160 


Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu 
                165                 170                 175     


Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
            180                 185                 190         


Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val 
        195                 200                 205             


Asp Lys Lys Val Glu Pro Lys Ser Cys Gly Gly Gly Gly Ser Phe Trp 
    210                 215                 220                 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
225                 230                 235                 240 


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu 
                245                 250                 255     


Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr 
            260                 265                 270         


Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr 
        275                 280                 285             


Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
    290                 295                 300                 


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
305                 310                 315                 320 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
                325                 330                 335     


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
            340                 345                 350         


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
        355                 360                 365             


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
    370                 375                 380                 


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
385                 390                 395                 400 


Pro Arg 
        


<210>  63
<211>  217
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA-Fab heavy chain

<400>  63

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Ala Val Ser Gly Phe Ile Phe Ser Asn Tyr 
            20                  25                  30          


Asp Met Ala Trp Val Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser His Asp Gly Arg Asn Thr Asn Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Gly Ser Arg Asp Ser Ala Gln Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Ile Tyr Phe Cys 
                85                  90                  95      


Ala Gly Pro Gly Phe Ala His Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser 
        115                 120                 125             


Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys 
    130                 135                 140                 


Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
145                 150                 155                 160 


Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu 
                165                 170                 175     


Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr 
            180                 185                 190         


Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val 
        195                 200                 205             


Asp Lys Lys Val Glu Pro Lys Ser Cys 
    210                 215         


<210>  64
<211>  219
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA-Fab light chain

<400>  64

Asp Ile Val Leu Thr Gln Ala Pro Leu Ser Val Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Ser Ala Ser Ile Ser Cys Arg Ser Ser Lys Thr Leu Leu Asn Thr 
            20                  25                  30          


Arg Gly Ile Thr Ser Leu Tyr Trp Tyr Leu Gln Lys Pro Gly Lys Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Ile Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Glu Thr His Phe Thr Leu Gln Ile 
65                  70                  75                  80  


Ser Lys Val Glu Thr Glu Asp Val Gly Ile Tyr Tyr Cys Ala Gln Phe 
                85                  90                  95      


Leu Glu Phe Pro Leu Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  65
<211>  114
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA VH

<400>  65

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Ala Val Ser Gly Phe Ile Phe Ser Asn Tyr 
            20                  25                  30          


Asp Met Ala Trp Val Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser His Asp Gly Arg Asn Thr Asn Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Gly Ser Arg Asp Ser Ala Gln Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Ile Tyr Phe Cys 
                85                  90                  95      


Ala Gly Pro Gly Phe Ala His Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  66
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-HA VL

<400>  66

Asp Ile Val Leu Thr Gln Ala Pro Leu Ser Val Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Ser Ala Ser Ile Ser Cys Arg Ser Ser Lys Thr Leu Leu Asn Thr 
            20                  25                  30          


Arg Gly Ile Thr Ser Leu Tyr Trp Tyr Leu Gln Lys Pro Gly Lys Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Ile Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Glu Thr His Phe Thr Leu Gln Ile 
65                  70                  75                  80  


Ser Lys Val Glu Thr Glu Asp Val Gly Ile Tyr Tyr Cys Ala Gln Phe 
                85                  90                  95      


Leu Glu Phe Pro Leu Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  67
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-Biotin CDR H1 Kabat

<400>  67

Gly Phe Asn Asn Lys Asp Thr Phe Phe Gln 
1               5                   10  


<210>  68
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-Biotin CDR H2 Kabat

<400>  68

Arg Ile Asp Pro Ala Asn Gly Phe Thr Lys Tyr Ala Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  69
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti- Biotin CDR H3 Kabat

<400>  69

Trp Asp Thr Tyr Gly Ala Ala Trp Phe Ala Tyr 
1               5                   10      


<210>  70
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti- Biotin CDR L1 Kabat

<400>  70

Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Ser 
1               5                   10      


<210>  71
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti- Biotin CDR L2 Kabat

<400>  71

Ser Ala Lys Thr Leu Ala Asp 
1               5           


<210>  72
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti- Biotin CDR L3 Kabat

<400>  72

Gln His Phe Trp Ser Ser Ile Tyr Thr 
1               5                   


<210>  73
<211>  433
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti- Biotin -scFv-CD28ATD-CD28CSD-CD3zSSD fusion

<400>  73

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Phe Asn Asn Lys Asp Thr 
            20                  25                  30          


Phe Phe Gln Trp Val Arg Gln Ala Pro Gly Gln Cys Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Ala Asn Gly Phe Thr Lys Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Asp Thr Tyr Gly Ala Ala Trp Phe Ala Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
    130                 135                 140                 


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
145                 150                 155                 160 


Ile Thr Cys Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Ser Trp Tyr 
                165                 170                 175     


Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile Tyr Ser Ala Lys 
            180                 185                 190         


Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Val Ala 
    210                 215                 220                 


Thr Tyr Tyr Cys Gln His Phe Trp Ser Ser Ile Tyr Thr Phe Gly Cys 
225                 230                 235                 240 


Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Phe Trp Val 
                245                 250                 255     


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
            260                 265                 270         


Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu 
        275                 280                 285             


His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg 
    290                 295                 300                 


Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
305                 310                 315                 320 


Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
                325                 330                 335     


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            340                 345                 350         


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
        355                 360                 365             


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    370                 375                 380                 


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
385                 390                 395                 400 


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                405                 410                 415     


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            420                 425                 430         


Arg 
    


<210>  74
<211>  247
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-Biotin-scFv

<400>  74

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Phe Asn Asn Lys Asp Thr 
            20                  25                  30          


Phe Phe Gln Trp Val Arg Gln Ala Pro Gly Gln Cys Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Ala Asn Gly Phe Thr Lys Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Asp Thr Tyr Gly Ala Ala Trp Phe Ala Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met 
    130                 135                 140                 


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
145                 150                 155                 160 


Ile Thr Cys Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Ser Trp Tyr 
                165                 170                 175     


Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile Tyr Ser Ala Lys 
            180                 185                 190         


Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Val Ala 
    210                 215                 220                 


Thr Tyr Tyr Cys Gln His Phe Trp Ser Ser Ile Tyr Thr Phe Gly Cys 
225                 230                 235                 240 


Gly Thr Lys Leu Glu Ile Lys 
                245         


<210>  75
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-Biotin VH

<400>  75

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Phe Asn Asn Lys Asp Thr 
            20                  25                  30          


Phe Phe Gln Trp Val Arg Gln Ala Pro Gly Gln Cys Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Ala Asn Gly Phe Thr Lys Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Asp Thr Tyr Gly Ala Ala Trp Phe Ala Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  76
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-Biotin VL

<400>  76

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gly Asn Ile His Asn Tyr 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Lys Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Val Ala Thr Tyr Tyr Cys Gln His Phe Trp Ser Ser Ile Tyr 
                85                  90                  95      


Thr Phe Gly Cys Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  77
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc CDR H1 Kabat

<400>  77

His Tyr Gly Met Ser 
1               5   


<210>  78
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc CDR H2 Kabat

<400>  78

Thr Ile Gly Ser Arg Gly Thr Tyr Thr His Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  79
<211>  18
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc CDR H3 Kabat

<400>  79

Arg Ser Glu Phe Tyr Tyr Tyr Gly Asn Thr Tyr Tyr Tyr Ser Ala Met 
1               5                   10                  15      


Asp Tyr 
        


<210>  80
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc CDR L1 Kabat

<400>  80

Arg Ala Ser Glu Ser Val Asp Asn Tyr Gly Phe Ser Phe Met Asn 
1               5                   10                  15  


<210>  81
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc CDR L2 Kabat

<400>  81

Ala Ile Ser Asn Arg Gly Ser 
1               5           


<210>  82
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc CDR L3 Kabat

<400>  82

Gln Gln Thr Lys Glu Val Pro Trp Thr 
1               5                   


<210>  83
<211>  414
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc-Fab-heavy chain- CD28ATD-CD28CSD-CD3zSSD fusion

<400>  83

Glu Val His Leu Val Glu Ser Gly Gly Asp Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser His Tyr 
            20                  25                  30          


Gly Met Ser Trp Val Arg Gln Thr Pro Asp Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Gly Ser Arg Gly Thr Tyr Thr His Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Asp Lys Asn Ala Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Ser Glu Phe Tyr Tyr Tyr Gly Asn Thr Tyr Tyr Tyr Ser 
            100                 105                 110         


Ala Met Asp Tyr Trp Gly Gln Gly Ala Ser Val Thr Val Ser Ser Ala 
        115                 120                 125             


Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala Ala 
    130                 135                 140                 


Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe 
145                 150                 155                 160 


Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly 
                165                 170                 175     


Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser 
            180                 185                 190         


Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr Val Thr 
        195                 200                 205             


Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile 
    210                 215                 220                 


Val Pro Arg Asp Cys Gly Gly Gly Gly Ser Phe Trp Val Leu Val Val 
225                 230                 235                 240 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                245                 250                 255     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            260                 265                 270         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        275                 280                 285             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val 
    290                 295                 300                 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
305                 310                 315                 320 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
                325                 330                 335     


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
            340                 345                 350         


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
        355                 360                 365             


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
    370                 375                 380                 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
385                 390                 395                 400 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                405                 410                 


<210>  84
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc-Fab heavy chain

<400>  84

Glu Val His Leu Val Glu Ser Gly Gly Asp Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser His Tyr 
            20                  25                  30          


Gly Met Ser Trp Val Arg Gln Thr Pro Asp Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Gly Ser Arg Gly Thr Tyr Thr His Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Asp Lys Asn Ala Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Ser Glu Phe Tyr Tyr Tyr Gly Asn Thr Tyr Tyr Tyr Ser 
            100                 105                 110         


Ala Met Asp Tyr Trp Gly Gln Gly Ala Ser Val Thr Val Ser Ser Ala 
        115                 120                 125             


Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala Ala 
    130                 135                 140                 


Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe 
145                 150                 155                 160 


Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly 
                165                 170                 175     


Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser 
            180                 185                 190         


Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr Val Thr 
        195                 200                 205             


Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile 
    210                 215                 220                 


Val Pro Arg Asp Cys 
225                 


<210>  85
<211>  218
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc-Fab light chain

<400>  85

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Phe Ser Phe Met Asn Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ile Ser Asn Arg Gly Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Asp Asp Pro Ala Met Tyr Phe Cys Gln Gln Thr Lys 
                85                  90                  95      


Glu Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 110         


Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu Gln 
        115                 120                 125             


Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe Tyr 
    130                 135                 140                 


Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg Gln 
145                 150                 155                 160 


Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser Thr 
                165                 170                 175     


Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu Arg 
            180                 185                 190         


His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser Pro 
        195                 200                 205             


Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
    210                 215             


<210>  86
<211>  127
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc VH

<400>  86

Glu Val His Leu Val Glu Ser Gly Gly Asp Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser His Tyr 
            20                  25                  30          


Gly Met Ser Trp Val Arg Gln Thr Pro Asp Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Gly Ser Arg Gly Thr Tyr Thr His Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Asp Lys Asn Ala Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg Ser Glu Phe Tyr Tyr Tyr Gly Asn Thr Tyr Tyr Tyr Ser 
            100                 105                 110         


Ala Met Asp Tyr Trp Gly Gln Gly Ala Ser Val Thr Val Ser Ser 
        115                 120                 125         


<210>  87
<211>  111
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-myc VL

<400>  87

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Phe Ser Phe Met Asn Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ile Ser Asn Arg Gly Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Asp Asp Pro Ala Met Tyr Phe Cys Gln Gln Thr Lys 
                85                  90                  95      


Glu Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  88
<211>  107
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C kappa

<400>  88

Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
1               5                   10                  15      


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
        35                  40                  45              


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
65                  70                  75                  80  


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
                85                  90                  95      


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
            100                 105         


<210>  89
<211>  102
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CH1 (mouse)

<400>  89

Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala 
1               5                   10                  15      


Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu 
    50                  55                  60                  


Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr Val 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys 
                85                  90                  95      


Ile Val Pro Arg Asp Cys 
            100         


<210>  90
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4 CDR H1 Kabat

<400>  90

Asp Tyr Gly Val Asn 
1               5   


<210>  91
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4 CDR H2 Kabat

<400>  91

Val Ile Trp Gly Asp Gly Ile Thr Asp His Asn Ser Ala Leu Lys Ser 
1               5                   10                  15      


<210>  92
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4 CDR H3 Kabat

<400>  92

Gly Leu Phe Asp Tyr 
1               5   


<210>  93
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4 CDR L1 Kabat

<400>  93

Arg Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Ser 
1               5                   10                  


<210>  94
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4 CDR L2 Kabat

<400>  94

Gly Thr Asn Asn Arg Ala Pro 
1               5           


<210>  95
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4 CDR L3 Kabat

<400>  95

Val Leu Trp Tyr Ser Asn His Trp Val 
1               5                   


<210>  96
<211>  428
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4-scFv-CD28ATD-CD28CSD-CD3zSSD fusion

<400>  96

Asp Ala Val Val Thr Gln Glu Ser Ala Leu Thr Ser Ser Pro Gly Glu 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Ser Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala 
65                  70                  75                  80  


Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Val Leu Trp Tyr Ser Asn 
                85                  90                  95      


His Trp Val Leu Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Ala Pro 
    130                 135                 140                 


Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 
145                 150                 155                 160 


Asp Tyr Gly Val Asn Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu 
                165                 170                 175     


Trp Leu Gly Val Ile Trp Gly Asp Gly Ile Thr Asp His Asn Ser Ala 
            180                 185                 190         


Leu Lys Ser Arg Leu Ser Val Thr Lys Asp Asn Ser Lys Ser Gln Val 
        195                 200                 205             


Phe Leu Lys Met Ser Ser Leu Gln Ser Gly Asp Ser Ala Arg Tyr Tyr 
    210                 215                 220                 


Cys Val Thr Gly Leu Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
225                 230                 235                 240 


Val Ser Ser Gly Gly Gly Gly Ser Phe Trp Val Leu Val Val Val Gly 
                245                 250                 255     


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
            260                 265                 270         


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
        275                 280                 285             


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
    290                 295                 300                 


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe 
305                 310                 315                 320 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
                325                 330                 335     


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
            340                 345                 350         


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
        355                 360                 365             


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
    370                 375                 380                 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
385                 390                 395                 400 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
                405                 410                 415     


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            420                 425             


<210>  97
<211>  243
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4-scFv

<400>  97

Asp Ala Val Val Thr Gln Glu Ser Ala Leu Thr Ser Ser Pro Gly Glu 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Ser Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala 
65                  70                  75                  80  


Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Val Leu Trp Tyr Ser Asn 
                85                  90                  95      


His Trp Val Leu Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Ala Pro 
    130                 135                 140                 


Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 
145                 150                 155                 160 


Asp Tyr Gly Val Asn Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu 
                165                 170                 175     


Trp Leu Gly Val Ile Trp Gly Asp Gly Ile Thr Asp His Asn Ser Ala 
            180                 185                 190         


Leu Lys Ser Arg Leu Ser Val Thr Lys Asp Asn Ser Lys Ser Gln Val 
        195                 200                 205             


Phe Leu Lys Met Ser Ser Leu Gln Ser Gly Asp Ser Ala Arg Tyr Tyr 
    210                 215                 220                 


Cys Val Thr Gly Leu Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
225                 230                 235                 240 


Val Ser Ser 
            


<210>  98
<211>  113
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4 VH

<400>  98

Asp Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Asp Tyr 
            20                  25                  30          


Gly Val Asn Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Asp Gly Ile Thr Asp His Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Ser Val Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Ser Ser Leu Gln Ser Gly Asp Ser Ala Arg Tyr Tyr Cys Val 
                85                  90                  95      


Thr Gly Leu Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser 
            100                 105                 110         


Ser 
    


<210>  99
<211>  109
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-GCN4 VL

<400>  99

Asp Ala Val Val Thr Gln Glu Ser Ala Leu Thr Ser Ser Pro Gly Glu 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Ser Trp Val Gln Glu Lys Pro Asp His Leu Phe Thr Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Asn Arg Ala Pro Gly Val Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Ile Gly Asp Lys Ala Ala Leu Thr Ile Thr Gly Ala 
65                  70                  75                  80  


Gln Thr Glu Asp Glu Ala Ile Tyr Phe Cys Val Leu Trp Tyr Ser Asn 
                85                  90                  95      


His Trp Val Leu Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  100
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HA tag

<400>  100

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  101
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Myc tag

<400>  101

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  102
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GCN4 tag

<400>  102

Tyr His Leu Glu Asn Glu Val Ala Arg Leu Lys Lys 
1               5                   10          


<210>  103
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  AviTag

<400>  103

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His 
1               5                   10                  


<210>  104
<211>  164
<212>  PRT
<213>  Homo sapiens

<400>  104

Met Lys Trp Lys Ala Leu Phe Thr Ala Ala Ile Leu Gln Ala Gln Leu 
1               5                   10                  15      


Pro Ile Thr Glu Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys 
            20                  25                  30          


Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu Thr Ala 
        35                  40                  45              


Leu Phe Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
    50                  55                  60                  


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
65                  70                  75                  80  


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
                85                  90                  95      


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
            100                 105                 110         


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
        115                 120                 125             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    130                 135                 140                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
145                 150                 155                 160 


Leu Pro Pro Arg 
                


<210>  105
<211>  492
<212>  DNA
<213>  Homo sapiens

<400>  105
atgaagtgga aggcgctttt caccgcggcc atcctgcagg cacagttgcc gattacagag       60

gcacagagct ttggcctgct ggatcccaaa ctctgctacc tgctggatgg aatcctcttc      120

atctatggtg tcattctcac tgccttgttc ctgagagtga agttcagcag gagcgcagag      180

ccccccgcgt accagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga      240

gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg      300

agaaggaaga accctcagga aggcctgtac aatgaactgc agaaagataa gatggcggag      360

gcctacagtg agattgggat gaaaggcgag cgccggaggg gcaaggggca cgatggcctt      420

taccagggtc tcagtacagc caccaaggac acctacgacg cccttcacat gcaggccctg      480

ccccctcgct aa                                                          492


<210>  106
<211>  164
<212>  PRT
<213>  Mus musculus

<400>  106

Met Lys Trp Lys Val Ser Val Leu Ala Cys Ile Leu His Val Arg Phe 
1               5                   10                  15      


Pro Gly Ala Glu Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys 
            20                  25                  30          


Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Ile Thr Ala 
        35                  40                  45              


Leu Tyr Leu Arg Ala Lys Phe Ser Arg Ser Ala Glu Thr Ala Ala Asn 
    50                  55                  60                  


Leu Gln Asp Pro Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
65                  70                  75                  80  


Glu Glu Tyr Asp Val Leu Glu Lys Lys Arg Ala Arg Asp Pro Glu Met 
                85                  90                  95      


Gly Gly Lys Gln Gln Arg Arg Arg Asn Pro Gln Glu Gly Val Tyr Asn 
            100                 105                 110         


Ala Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Thr 
        115                 120                 125             


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
    130                 135                 140                 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Thr 
145                 150                 155                 160 


Leu Ala Pro Arg 
                


<210>  107
<211>  495
<212>  DNA
<213>  Mus musculus

<400>  107
atgaagtgga aagtgtctgt tctcgcctgc atcctccacg tgcggttccc aggagcagag       60

gcacagagct ttggtctgct ggatcccaaa ctctgctact tgctagatgg aatcctcttc      120

atctacggag tcatcatcac agccctgtac ctgagagcaa aattcagcag gagtgcagag      180

actgctgcca acctgcagga ccccaaccag ctctacaatg agctcaatct agggcgaaga      240

gaggaatatg acgtcttgga gaagaagcgg gctcgggatc cagagatggg aggcaaacag      300

cagaggagga ggaaccccca ggaaggcgta tacaatgcac tgcagaaaga caagatggca      360

gaagcctaca gtgagatcgg cacaaaaggc gagaggcgga gaggcaaggg gcacgatggc      420

ctttaccagg gtctcagcac tgccaccaag gacacctatg atgccctgca tatgcagacc      480

ctggcccctc gctaa                                                       495


<210>  108
<211>  660
<212>  DNA
<213>  Homo sapiens

<400>  108
atgctgcgcc tgctgctggc gctgaacctg tttccgagca ttcaggtgac cggcaacaaa       60

attctggtga aacagagccc gatgctggtg gcgtatgata acgcggtgaa cctgagctgc      120

aaatatagct ataacctgtt tagccgcgaa tttcgcgcga gcctgcataa aggcctggat      180

agcgcggtgg aagtgtgcgt ggtgtatggc aactatagcc agcagctgca ggtgtatagc      240

aaaaccggct ttaactgcga tggcaaactg ggcaacgaaa gcgtgacctt ttatctgcag      300

aacctgtatg tgaaccagac cgatatttat ttttgcaaaa ttgaagtgat gtatccgccg      360

ccgtatctgg ataacgaaaa aagcaacggc accattattc atgtgaaagg caaacatctg      420

tgcccgagcc cgctgtttcc gggcccgagc aaaccgtttt gggtgctggt ggtggtgggc      480

ggcgtgctgg cgtgctatag cctgctggtg accgtggcgt ttattatttt ttgggtgcgc      540

agcaaacgca gccgcctgct gcatagcgat tatatgaaca tgaccccgcg ccgcccgggc      600

ccgacccgca aacattatca gccgtatgcg ccgccgcgcg attttgcggc gtatcgcagc      660


<210>  109
<211>  220
<212>  PRT
<213>  Homo sapiens

<400>  109

Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      


Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr 
            20                  25                  30          


Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser 
        35                  40                  45              


Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu 
    50                  55                  60                  


Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser 
65                  70                  75                  80  


Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr 
                85                  90                  95      


Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys 
            100                 105                 110         


Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
        115                 120                 125             


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
    130                 135                 140                 


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
145                 150                 155                 160 


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                165                 170                 175     


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
            180                 185                 190         


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
        195                 200                 205             


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
    210                 215                 220 


<210>  110
<211>  654
<212>  DNA
<213>  Mus musculus

<400>  110
atgaccctgc gcctgctgtt tctggcgctg aactttttta gcgtgcaggt gaccgaaaac       60

aaaattctgg tgaaacagag cccgctgctg gtggtggata gcaacgaagt gagcctgagc      120

tgccgctata gctataacct gctggcgaaa gaatttcgcg cgagcctgta taaaggcgtg      180

aacagcgatg tggaagtgtg cgtgggcaac ggcaacttta cctatcagcc gcagtttcgc      240

agcaacgcgg aatttaactg cgatggcgat tttgataacg aaaccgtgac ctttcgcctg      300

tggaacctgc atgtgaacca taccgatatt tatttttgca aaattgaatt tatgtatccg      360

ccgccgtatc tggataacga acgcagcaac ggcaccatta ttcatattaa agaaaaacat      420

ctgtgccata cccagagcag cccgaaactg ttttgggcgc tggtggtggt ggcgggcgtg      480

ctgttttgct atggcctgct ggtgaccgtg gcgctgtgcg tgatttggac caacagccgc      540

cgcaaccgcc tgctgcagag cgattatatg aacatgaccc cgcgccgccc gggcctgacc      600

cgcaaaccgt atcagccgta tgcgccggcg cgcgattttg cggcgtatcg cccg            654


<210>  111
<211>  218
<212>  PRT
<213>  Mus musculus

<400>  111

Met Thr Leu Arg Leu Leu Phe Leu Ala Leu Asn Phe Phe Ser Val Gln 
1               5                   10                  15      


Val Thr Glu Asn Lys Ile Leu Val Lys Gln Ser Pro Leu Leu Val Val 
            20                  25                  30          


Asp Ser Asn Glu Val Ser Leu Ser Cys Arg Tyr Ser Tyr Asn Leu Leu 
        35                  40                  45              


Ala Lys Glu Phe Arg Ala Ser Leu Tyr Lys Gly Val Asn Ser Asp Val 
    50                  55                  60                  


Glu Val Cys Val Gly Asn Gly Asn Phe Thr Tyr Gln Pro Gln Phe Arg 
65                  70                  75                  80  


Ser Asn Ala Glu Phe Asn Cys Asp Gly Asp Phe Asp Asn Glu Thr Val 
                85                  90                  95      


Thr Phe Arg Leu Trp Asn Leu His Val Asn His Thr Asp Ile Tyr Phe 
            100                 105                 110         


Cys Lys Ile Glu Phe Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Arg 
        115                 120                 125             


Ser Asn Gly Thr Ile Ile His Ile Lys Glu Lys His Leu Cys His Thr 
    130                 135                 140                 


Gln Ser Ser Pro Lys Leu Phe Trp Ala Leu Val Val Val Ala Gly Val 
145                 150                 155                 160 


Leu Phe Cys Tyr Gly Leu Leu Val Thr Val Ala Leu Cys Val Ile Trp 
                165                 170                 175     


Thr Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met 
            180                 185                 190         


Thr Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala 
        195                 200                 205             


Pro Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
    210                 215             


<210>  112
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CD28 YMNM

<400>  112

Tyr Met Asn Met 
1               


<210>  113
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CD28 PYAP

<400>  113

Pro Tyr Ala Pro 
1               


<210>  114
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Signal peptide

<400>  114

Ala Thr Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala 
1               5                   10                  15      


Thr Gly Val His Ser 
            20      


<210>  115
<211>  57
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Signal peptide DNA sequence

<400>  115
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcactcc          57


<210>  116
<211>  449
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-CD20 (GA101) heavy chain

<400>  116

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Tyr Ser 
            20                  25                  30          


Trp Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Phe Pro Gly Asp Gly Asp Thr Asp Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Val Phe Asp Gly Tyr Trp Leu Val Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
                325                 330                 335     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
        355                 360                 365             


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            420                 425                 430         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             


Lys 
    


<210>  117
<211>  219
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-CD20 (GA101) light chain

<400>  117

Asp Ile Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
            20                  25                  30          


Asn Gly Ile Thr Tyr Leu Tyr Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Gln Met Ser Asn Leu Val Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ala Gln Asn 
                85                  90                  95      


Leu Glu Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  118
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-CD3 HCDR1 Kabat

<400>  118

Thr Tyr Ala Met Asn 
1               5   


<210>  119
<211>  19
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-CD3 HCDR2 Kabat

<400>  119

Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser 
1               5                   10                  15      


Val Lys Gly 
            


<210>  120
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-CD3 HCDR3 Kabat

<400>  120

His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Phe Ala Tyr 
1               5                   10                  


<210>  121
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-CD3 LCDR1 Kabat

<400>  121

Gly Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn 
1               5                   10                  


<210>  122
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-CD3 LCDR2 Kabat

<400>  122

Gly Thr Asn Lys Arg Ala Pro 
1               5           


<210>  123
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Anti-CD3 LCDR3 Kabat

<400>  123

Ala Leu Trp Tyr Ser Asn Leu Trp Val 
1               5                   


