                         SEQUENCE LISTING

<110>  The Board of Trustees of the Leland Stanford Junior 
       University
 
<120>  LIGANDS OF THE UROKINASE RECEPTOR AND THEIR USE IN TREATING, 
       DETECTING, AND IMAGING CANCER

<130>  S18-016

<150>  62/635,509
<151>  2018-02-26

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  46
<212>  PRT
<213>  Mus musculus

<400>  1

Gly Ser Val Leu Gly Ala Pro Asp Glu Ser Asn Cys Gly Cys Gln Asn 
1               5                   10                  15      


Gly Gly Val Cys Val Ser Tyr Lys Tyr Phe Ser Arg Ile Arg Arg Cys 
            20                  25                  30          


Ser Cys Pro Arg Lys Phe Gln Gly Glu His Cys Glu Ile Asp 
        35                  40                  45      


<210>  2
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  *mGFD

<400>  2

Gly Ser Val Leu Gly Ala Pro Asp Glu Ser Asn Cys Gly Cys Gln Asn 
1               5                   10                  15      


Gly Gly Val Cys Val Ser Tyr Lys Tyr Phe Ser Arg Ile Trp Arg Cys 
            20                  25                  30          


Ser Cys Pro Arg Gly Phe Arg Gly Arg Tyr Cys Glu Ile Asp 
        35                  40                  45      


<210>  3
<211>  48
<212>  PRT
<213>  Homo sapiens

<400>  3

Ser Asn Glu Leu His Gln Val Pro Ser Asn Cys Asp Cys Leu Asn Gly 
1               5                   10                  15      


Gly Thr Cys Val Ser Asn Lys Tyr Phe Ser Asn Ile His Trp Cys Asn 
            20                  25                  30          


Cys Pro Lys Lys Phe Gly Gly Gln His Cys Glu Ile Asp Lys Ser Lys 
        35                  40                  45              


<210>  4
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  *hGFD

<400>  4

Ser Asn Glu Trp His Arg Val Pro Ser Asn Cys Asp Cys Leu Asn Gly 
1               5                   10                  15      


Gly Thr Cys Val Ser Asn Lys Tyr Phe Ser Asn Ile Gln Trp Cys Asn 
            20                  25                  30          


Cys Pro Lys Gly Phe Gly Gly Arg Arg Cys Glu Ile Asp Arg Ser Lys 
        35                  40                  45              


<210>  5
<211>  44
<212>  PRT
<213>  Mus musculus

<400>  5

Asp Gln Glu Ser Cys Lys Gly Arg Cys Thr Gln Gly Phe Met Ala Ser 
1               5                   10                  15      


Lys Lys Cys Gln Cys Asp Glu Leu Cys Thr Tyr Tyr Gln Ser Cys Cys 
            20                  25                  30          


Ala Asp Tyr Met Glu Gln Cys Lys Pro Gln Val Thr 
        35                  40                  


<210>  6
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  *mSMB

<400>  6

Asn Gln Gly Ser Cys Lys Gly Arg Cys Thr Gln Gly Phe Val Ala Ser 
1               5                   10                  15      


Glu Lys Cys Gln Cys Asp Glu Leu Cys Thr Tyr Tyr Gln Ser Cys Cys 
            20                  25                  30          


Ala Asp Tyr Met Glu Gln Cys Lys Pro Gln Val Thr 
        35                  40                  


<210>  7
<211>  44
<212>  PRT
<213>  Homo sapiens

<400>  7

Asp Gln Glu Ser Cys Lys Gly Arg Cys Thr Glu Gly Phe Asn Val Asp 
1               5                   10                  15      


Lys Lys Cys Gln Cys Asp Glu Leu Cys Ser Tyr Tyr Gln Ser Cys Cys 
            20                  25                  30          


Thr Asp Tyr Thr Ala Glu Cys Lys Pro Gln Val Thr 
        35                  40                  


<210>  8
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  *hSMB

<400>  8

Gly Gln Gly Ser Cys Lys Gly Arg Cys Thr Glu Gly Phe Asn Val Asp 
1               5                   10                  15      


Lys Glu Cys Gln Cys Asp Glu Leu Cys Ser Tyr Tyr Gln Ser Cys Cys 
            20                  25                  30          


Thr Asp Tyr Thr Ala Glu Cys Lys Ser Gln Val Thr 
        35                  40                  


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  9

Gly Gly Gly Gly Ser 
1               5   


<210>  10
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG tag

<400>  10

Gly Gly Gly Gly Ser Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5                   10              


<210>  11
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  [Gly4Ser]5 linker

<400>  11

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  12
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mGS bispecific uPAR antagonist

<400>  12

Gly Ser Val Leu Gly Ala Pro Asp Glu Ser Asn Cys Gly Cys Gln Asn 
1               5                   10                  15      


Gly Gly Val Cys Val Ser Tyr Lys Tyr Phe Ser Arg Ile Trp Arg Cys 
            20                  25                  30          


Ser Cys Pro Arg Gly Phe Arg Gly Arg Tyr Cys Glu Ile Asp Gly Gly 
        35                  40                  45              


Gly Gly Asn Gly Gly Gly Gly Ser Gly Arg Gly Gly Ser Gly Gly Gly 
    50                  55                  60                  


Ser Ser Gly Gly Gly Gly Ser Asn Gln Gly Ser Cys Lys Gly Arg Cys 
65                  70                  75                  80  


Thr Gln Gly Phe Val Ala Ser Glu Lys Cys Gln Cys Asp Glu Leu Cys 
                85                  90                  95      


Thr Tyr Tyr Gln Ser Cys Cys Ala Asp Tyr Met Glu Gln Cys Lys Pro 
            100                 105                 110         


Gln Val Thr 
        115 


<210>  13
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hGS bispecific uPAR antagonist

<400>  13

Ser Asn Glu Trp His Arg Val Pro Ser Asn Cys Asp Cys Leu Asn Gly 
1               5                   10                  15      


Gly Thr Cys Val Ser Asn Lys Tyr Phe Ser Asn Ile Gln Trp Cys Asn 
            20                  25                  30          


Cys Pro Lys Gly Phe Gly Gly Arg Arg Cys Glu Ile Asp Arg Ser Lys 
        35                  40                  45              


Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Gly Gly Gly Ser Gly 
    50                  55                  60                  


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gln Gly Ser Cys Lys Gly 
65                  70                  75                  80  


Arg Cys Thr Glu Gly Phe Asn Val Asp Lys Glu Cys Gln Cys Asp Glu 
                85                  90                  95      


Leu Cys Ser Tyr Tyr Gln Ser Cys Cys Thr Asp Tyr Thr Ala Glu Cys 
            100                 105                 110         


Lys Ser 
        


<210>  14
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  murine engineered linker

<400>  14

Gly Gly Gly Gly Asn Gly Gly Gly Gly Ser Gly Arg Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  15
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human engineered linker

<400>  15

Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  16

Gly Gly Gly Ser 
1               


<210>  17
<211>  373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mGS-Fc (mIgG2a)

<400>  17

Met Lys Val Trp Leu Ala Ser Leu Phe Leu Cys Ala Leu Val Val Lys 
1               5                   10                  15      


Asn Ser Glu Gly Gly Ser Val Leu Gly Ala Pro Asp Glu Ser Asn Cys 
            20                  25                  30          


Gly Cys Gln Asn Gly Gly Val Cys Val Ser Tyr Lys Tyr Phe Ser Arg 
        35                  40                  45              


Ile Trp Arg Cys Ser Cys Pro Arg Gly Phe Arg Gly Arg Tyr Cys Glu 
    50                  55                  60                  


Ile Asp Gly Gly Gly Gly Asn Gly Gly Gly Gly Ser Gly Arg Gly Gly 
65                  70                  75                  80  


Ser Gly Gly Gly Ser Ser Gly Gly Gly Gly Ser Asn Gln Gly Ser Cys 
                85                  90                  95      


Lys Gly Arg Cys Thr Gln Gly Phe Val Ala Ser Glu Lys Cys Gln Cys 
            100                 105                 110         


Asp Glu Leu Cys Thr Tyr Tyr Gln Ser Cys Cys Ala Asp Tyr Met Glu 
        115                 120                 125             


Gln Cys Lys Pro Gln Val Thr Gly Gly Gly Gly Ser Glu Pro Arg Gly 
    130                 135                 140                 


Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu 
145                 150                 155                 160 


Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val 
                165                 170                 175     


Leu Met Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val 
            180                 185                 190         


Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val 
        195                 200                 205             


Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser 
    210                 215                 220                 


Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met 
225                 230                 235                 240 


Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala 
                245                 250                 255     


Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro 
            260                 265                 270         


Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln 
        275                 280                 285             


Val Thr Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr 
    290                 295                 300                 


Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr 
305                 310                 315                 320 


Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu 
                325                 330                 335     


Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser 
            340                 345                 350         


Val Val His Glu Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser 
        355                 360                 365             


Arg Thr Pro Gly Lys 
    370             


<210>  18
<211>  375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hGS-Fc (mIgG2a)

<400>  18

Met Arg Ala Leu Leu Ala Arg Leu Leu Leu Cys Val Leu Val Val Ser 
1               5                   10                  15      


Asp Ser Lys Gly Ser Asn Glu Trp His Arg Val Pro Ser Asn Cys Asp 
            20                  25                  30          


Cys Leu Asn Gly Gly Thr Cys Val Ser Asn Lys Tyr Phe Ser Asn Ile 
        35                  40                  45              


Gln Trp Cys Asn Cys Pro Lys Gly Phe Gly Gly Arg Arg Cys Glu Ile 
    50                  55                  60                  


Asp Arg Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Gly 
65                  70                  75                  80  


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gln Gly 
                85                  90                  95      


Ser Cys Lys Gly Arg Cys Thr Glu Gly Phe Asn Val Asp Lys Glu Cys 
            100                 105                 110         


Gln Cys Asp Glu Leu Cys Ser Tyr Tyr Gln Ser Cys Cys Thr Asp Tyr 
        115                 120                 125             


Thr Ala Glu Cys Lys Ser Gln Val Thr Gly Gly Gly Gly Ser Glu Pro 
    130                 135                 140                 


Arg Gly Pro Thr Ile Lys Pro Cys Pro Pro Cys Lys Cys Pro Ala Pro 
145                 150                 155                 160 


Asn Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Ile Lys 
                165                 170                 175     


Asp Val Leu Met Ile Ser Leu Ser Pro Ile Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn 
        195                 200                 205             


Asn Val Glu Val His Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr 
    210                 215                 220                 


Asn Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp 
225                 230                 235                 240 


Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu 
                245                 250                 255     


Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys Gly Ser Val Arg 
            260                 265                 270         


Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Glu Glu Glu Met Thr Lys 
        275                 280                 285             


Lys Gln Val Thr Leu Thr Cys Met Val Thr Asp Phe Met Pro Glu Asp 
    290                 295                 300                 


Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys 
305                 310                 315                 320 


Asn Thr Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser 
                325                 330                 335     


Lys Leu Arg Val Glu Lys Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser 
            340                 345                 350         


Cys Ser Val Val His Glu Gly Leu His Asn His His Thr Thr Lys Ser 
        355                 360                 365             


Phe Ser Arg Thr Pro Gly Lys 
    370                 375 


<210>  19
<211>  367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mGS-Fc (hIgG1)

<400>  19

Met Lys Val Trp Leu Ala Ser Leu Phe Leu Cys Ala Leu Val Val Lys 
1               5                   10                  15      


Asn Ser Glu Gly Gly Ser Val Leu Gly Ala Pro Asp Glu Ser Asn Cys 
            20                  25                  30          


Gly Cys Gln Asn Gly Gly Val Cys Val Ser Tyr Lys Tyr Phe Ser Arg 
        35                  40                  45              


Ile Trp Arg Cys Ser Cys Pro Arg Gly Phe Arg Gly Arg Tyr Cys Glu 
    50                  55                  60                  


Ile Asp Gly Gly Gly Gly Asn Gly Gly Gly Gly Ser Gly Arg Gly Gly 
65                  70                  75                  80  


Ser Gly Gly Gly Ser Ser Gly Gly Gly Gly Ser Asn Gln Gly Ser Cys 
                85                  90                  95      


Lys Gly Arg Cys Thr Gln Gly Phe Val Ala Ser Glu Lys Cys Gln Cys 
            100                 105                 110         


Asp Glu Leu Cys Thr Tyr Tyr Gln Ser Cys Cys Ala Asp Tyr Met Glu 
        115                 120                 125             


Gln Cys Lys Pro Gln Val Thr Gly Gly Gly Gly Ser Asp Lys Thr His 
    130                 135                 140                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
145                 150                 155                 160 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                165                 170                 175     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Gln 
            180                 185                 190         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Gln Val His Asn Ala Lys 
        195                 200                 205             


Thr Lys Pro Arg Glu Gln Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    210                 215                 220                 


Val Leu Thr Val Leu His Gln Asn Trp Leu Asp Gly Lys Glu Tyr Lys 
225                 230                 235                 240 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                245                 250                 255     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            260                 265                 270         


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        275                 280                 285             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    290                 295                 300                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
305                 310                 315                 320 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                325                 330                 335     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            340                 345                 350         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        355                 360                 365         


<210>  20
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hGS-Fc (hIgG1)

<400>  20

Met Arg Ala Leu Leu Ala Arg Leu Leu Leu Cys Val Leu Val Val Ser 
1               5                   10                  15      


Asp Ser Lys Gly Ser Asn Glu Trp His Arg Val Pro Ser Asn Cys Asp 
            20                  25                  30          


Cys Leu Asn Gly Gly Thr Cys Val Ser Asn Lys Tyr Phe Ser Asn Ile 
        35                  40                  45              


Gln Trp Cys Asn Cys Pro Lys Gly Phe Gly Gly Arg Arg Cys Glu Ile 
    50                  55                  60                  


Asp Arg Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Gly 
65                  70                  75                  80  


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gln Gly 
                85                  90                  95      


Ser Cys Lys Gly Arg Cys Thr Glu Gly Phe Asn Val Asp Lys Glu Cys 
            100                 105                 110         


Gln Cys Asp Glu Leu Cys Ser Tyr Tyr Gln Ser Cys Cys Thr Asp Tyr 
        115                 120                 125             


Thr Ala Glu Cys Lys Ser Gln Val Thr Gly Gly Gly Gly Ser Asp Lys 
    130                 135                 140                 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
145                 150                 155                 160 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                165                 170                 175     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            180                 185                 190         


Pro Gln Val Lys Phe Asn Trp Tyr Val Asp Gly Val Gln Val His Asn 
        195                 200                 205             


Ala Lys Thr Lys Pro Arg Glu Gln Gln Tyr Asn Ser Thr Tyr Arg Val 
    210                 215                 220                 


Val Ser Val Leu Thr Val Leu His Gln Asn Trp Leu Asp Gly Lys Glu 
225                 230                 235                 240 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
                245                 250                 255     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            260                 265                 270         


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
        275                 280                 285             


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    290                 295                 300                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
305                 310                 315                 320 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                325                 330                 335     


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            340                 345                 350         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        355                 360                 365             


Lys 
    


