﻿               SEQUENCE LISTING

<110> Technische Universiteit Eindhoven

<120> Bioluminescent biosensor for detecting and quantifying biomolecules or ligands in solution

<130> 77659PC

<150> US62/635053
<151> 2018-02-26

<160> 27

<170> BiSSAP 1.3.6

<210> 1
<211> 11
<212> PRT
<213> Oplophorus gracilirostris


<220> 
<223> SBiT with K(d) of 190 µM

<400> 1
Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
1               5                   10      

<210> 2
<211> 11
<212> PRT
<213> Oplophorus gracilirostris


<220> 
<223> SBiT with K(d) of 2.5 µM

<400> 2
Val Thr Gly Tyr Arg Leu Phe Glu Lys Glu Ser 
1               5                   10      

<210> 3
<211> 11
<212> PRT
<213> Oplophorus gracilirostris


<220> 
<223> SBiT with K(d) of 0.18 µM

<400> 3
Val Thr Gly Tyr Arg Leu Phe Glu Lys Ile Ser 
1               5                   10      

<210> 4
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> HIV1-p17-antibody binding epitope

<400> 4
Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg Pro 
1               5                   10          

<210> 5
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> Trastuzumab binding mimitope

<400> 5
Gln Leu Gly Pro Tyr Glu Leu Trp Glu Leu Ser His 
1               5                   10          

<210> 6
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> NB-LUMABS-1

<400> 6
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg 
225                 230                 235                 240 
Pro Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Glu Leu Asp Arg Trp Glu Lys 
        355                 360                 365             
Ile Arg Leu Arg Pro Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Glu Ser Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 7
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> NB-LUMABS-2

<400> 7
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg 
225                 230                 235                 240 
Pro Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Glu Leu Asp Arg Trp Glu Lys 
        355                 360                 365             
Ile Arg Leu Arg Pro Thr Ser Gly Gly Ser Gly Gly Ser Val Thr Gly 
    370                 375                 380                 
Tyr Arg Leu Phe Glu Lys Glu Ser Gly Gly Ser Cys Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 8
<211> 408
<212> PRT
<213> Artificial Sequence


<220> 
<223> NB-LUMABS-3

<400> 8
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg 
225                 230                 235                 240 
Pro Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Glu Leu Asp Arg Trp Glu Lys 
        355                 360                 365             
Ile Arg Leu Arg Pro Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Glu Ser Gly Gly Ser Cys Gly Gly Ser 
385                 390                 395                 400 
Trp Ser His Pro Gln Phe Glu Lys 
                405             

<210> 9
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> NB-LUMABS-4

<400> 9
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg 
225                 230                 235                 240 
Pro Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Glu Leu Asp Arg Trp Glu Lys 
        355                 360                 365             
Ile Arg Leu Arg Pro Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Ile Ser Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 10
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> NB-LUMABS-5

<400> 10
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg 
225                 230                 235                 240 
Pro Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Glu Leu Asp Arg Trp Glu Lys 
        355                 360                 365             
Ile Arg Leu Arg Pro Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Glu Ile Leu Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 11
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> NB-LUMABS-6

<400> 11
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Lys Glu Ser 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg 
225                 230                 235                 240 
Pro Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Glu Leu Asp Arg Trp Glu Lys 
        355                 360                 365             
Ile Arg Leu Arg Pro Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Glu Ser Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 12
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> NB-LUMABS-7

<400> 12
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Lys Glu Ser 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Glu Leu Asp Arg Trp Glu Lys Ile Arg Leu Arg 
225                 230                 235                 240 
Pro Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Glu Leu Asp Arg Trp Glu Lys 
        355                 360                 365             
Ile Arg Leu Arg Pro Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Ile Ser Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 13
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> TRAS-NB-LUMABS-1

<400> 13
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Gln Leu Gly Pro Tyr Glu Leu Trp Glu Leu Ser 
225                 230                 235                 240 
His Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Gln Leu Gly Pro Tyr Glu Leu 
        355                 360                 365             
Trp Glu Leu Ser His Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Glu Ser Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 14
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> TRAS-NB-LUMABS-2

<400> 14
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Lys Glu Ser 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Gln Leu Gly Pro Tyr Glu Leu Trp Glu Leu Ser 
225                 230                 235                 240 
His Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Gln Leu Gly Pro Tyr Glu Leu 
        355                 360                 365             
Trp Glu Leu Ser His Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Ile Ser Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 15
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> TRAS-NB-LUMABS-3

<400> 15
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Gln Leu Gly Pro Tyr Glu Leu Trp Glu Leu Ser 
225                 230                 235                 240 
His Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Gln Leu Gly Pro Tyr Glu Leu 
        355                 360                 365             
Trp Glu Leu Ser His Thr Ser Gly Gly Ser Cys Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Glu Ile Leu Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 16
<211> 56
<212> PRT
<213> streptococcus


<220> 
<223> Protein G

<220> 
<221> BINDING
<222> 24
<223> photocrosslinker p-benzoyl-l-phenylalanine (BPA)

<400> 16
Met Thr Phe Lys Leu Ile Ile Asn Gly Lys Thr Leu Lys Gly Glu Ile 
1               5                   10                  15      
Thr Ile Glu Ala Val Asp Ala Xaa Glu Ala Glu Lys Ile Phe Lys Gln 
            20                  25                  30          
Tyr Ala Asn Asp Tyr Gly Ile Asp Gly Glu Trp Thr Tyr Asp Asp Ala 
        35                  40                  45              
Thr Lys Thr Phe Thr Val Thr Glu 
    50                  55      

<210> 17
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CRP169 binding epitope

<400> 17
Val Gly Ala Glu Ala Ser Ile Ile Leu Gly Gln Glu Gln Asp Ser Phe 
1               5                   10                  15      
Gly 
    

<210> 18
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> Anti-CRP36 binding epitope

<400> 18
Tyr Ser Phe Thr Val Gly Gly Ser Glu Ile Leu Phe Glu Val Pro Glu 
1               5                   10                  15      
Val 
    

<210> 19
<211> 158
<212> PRT
<213> Oplophorus gracilirostris


<220> 
<223> LBiT

<400> 19
Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr Ala Ala 
1               5                   10                  15      
Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Leu 
            20                  25                  30          
Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val Arg Ser 
        35                  40                  45              
Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu 
    50                  55                  60                  
Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe Lys Val 
65                  70                  75                  80  
Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro Tyr Gly 
                85                  90                  95      
Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr Phe Gly 
            100                 105                 110         
Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val 
        115                 120                 125             
Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile 
    130                 135                 140                 
Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
145                 150                 155             

<210> 20
<211> 120
<212> PRT
<213> Artificial Sequence


<220> 
<223> semi-flexible linker

<400> 20
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      
Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          
Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
        35                  40                  45              
Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
    50                  55                  60                  
Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
65                  70                  75                  80  
Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
                85                  90                  95      
Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
            100                 105                 110         
Gly Gly Ser Gly Gly Ser Gly Gly 
        115                 120 

<210> 21
<211> 462
<212> PRT
<213> Artificial Sequence


<220> 
<223> pG-NB-LUMABS-17

<220> 
<221> BINDING
<222> 254
<223> photocrosslinker p-benzoyl-l-phenylalanine (BPA)

<400> 21
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Gly Gly Ser Gly Gly Ser Met Thr Phe Lys Leu Ile Ile Asn Gly Lys 
225                 230                 235                 240 
Thr Leu Lys Gly Glu Ile Thr Ile Glu Ala Val Asp Ala Xaa Glu Ala 
                245                 250                 255     
Glu Lys Ile Phe Lys Gln Tyr Ala Asn Asp Tyr Gly Ile Asp Gly Glu 
            260                 265                 270         
Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Gly Thr 
        275                 280                 285             
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
305                 310                 315                 320 
Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
                325                 330                 335     
Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
        355                 360                 365             
Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
    370                 375                 380                 
Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
385                 390                 395                 400 
Gly Gly Ser Gly Gly Ser Gly Gly Val Gly Ala Glu Ala Ser Ile Ile 
                405                 410                 415     
Leu Gly Gln Glu Gln Asp Ser Phe Gly Gly Gly Ser Gly Gly Ser Cys 
            420                 425                 430         
Thr Ser Gly Gly Ser Val Thr Gly Tyr Arg Leu Phe Glu Lys Glu Ser 
        435                 440                 445             
Gly Gly Ser Gly Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
    450                 455                 460         

<210> 22
<211> 462
<212> PRT
<213> Artificial Sequence


<220> 
<223> pG-NB-LUMABS-11

<220> 
<221> BINDING
<222> 254
<223> photocrosslinker p-benzoyl-l-phenylalanine (BPA)

<400> 22
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Gly Gly Ser Gly Gly Ser Met Thr Phe Lys Leu Ile Ile Asn Gly Lys 
225                 230                 235                 240 
Thr Leu Lys Gly Glu Ile Thr Ile Glu Ala Val Asp Ala Xaa Glu Ala 
                245                 250                 255     
Glu Lys Ile Phe Lys Gln Tyr Ala Asn Asp Tyr Gly Ile Asp Gly Glu 
            260                 265                 270         
Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Gly Thr 
        275                 280                 285             
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
305                 310                 315                 320 
Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
                325                 330                 335     
Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
        355                 360                 365             
Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
    370                 375                 380                 
Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
385                 390                 395                 400 
Gly Gly Ser Gly Gly Ser Gly Gly Tyr Ser Phe Thr Val Gly Gly Ser 
                405                 410                 415     
Glu Ile Leu Phe Glu Val Pro Glu Val Gly Gly Ser Gly Gly Ser Cys 
            420                 425                 430         
Thr Ser Gly Gly Ser Val Thr Gly Tyr Arg Leu Phe Glu Lys Glu Ser 
        435                 440                 445             
Gly Gly Ser Gly Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
    450                 455                 460         

<210> 23
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cetuximab binding meditope with monovalent K(d) of 61 nM

<400> 23
Cys Val Phe Asp Leu Gly Thr Arg Arg Leu Arg Cys 
1               5                   10          

<210> 24
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cetuximab binding meditope with monovalent Kd of 270 nM

<400> 24
Cys Gln Phe Asp Leu Ser Thr Arg Arg Leu Lys Cys 
1               5                   10          

<210> 25
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> CTX-NB-LUMABS-1

<220> 
<221> BINDING
<222> 379
<223> photocrosslinker p-azido-l-phenylalanine (pAzF)

<400> 25
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Cys Val Phe Asp Leu Gly Thr Arg Arg Leu Arg 
225                 230                 235                 240 
Cys Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Cys Val Phe Asp Leu Gly Thr 
        355                 360                 365             
Arg Arg Leu Arg Cys Thr Ser Gly Gly Ser Xaa Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Glu Ser Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 26
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> CTX-NB-LUMABS-2

<220> 
<221> BINDING
<222> 396
<223> photocrosslinker p-azido-l-phenylalanine (pAzF)

<400> 26
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Cys Val Phe Asp Leu Gly Thr Arg Arg Leu Arg 
225                 230                 235                 240 
Cys Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Cys Val Phe Asp Leu Gly Thr 
        355                 360                 365             
Arg Arg Leu Arg Cys Thr Ser Gly Gly Ser Gly Gly Ser Val Thr Gly 
    370                 375                 380                 
Tyr Arg Leu Phe Glu Lys Glu Ser Gly Gly Ser Xaa Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

<210> 27
<211> 407
<212> PRT
<213> Artificial Sequence


<220> 
<223> CTX-NB-LUMABS-3

<220> 
<221> BINDING
<222> 379
<223> photocrosslinker p-azido-l-phenylalanine (pAzF)

<400> 27
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      
Arg Gly Ser His Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
            20                  25                  30          
Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
        35                  40                  45              
Ser Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    50                  55                  60                  
Gly Ser Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr 
65                  70                  75                  80  
Ala Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser 
                85                  90                  95      
Leu Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val 
            100                 105                 110         
Arg Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro 
        115                 120                 125             
Tyr Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe 
    130                 135                 140                 
Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro 
145                 150                 155                 160 
Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr 
                165                 170                 175     
Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile 
            180                 185                 190         
Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg 
        195                 200                 205             
Leu Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
    210                 215                 220                 
Ser Gly Gly Gly Thr Cys Gln Phe Asp Leu Ser Thr Arg Arg Leu Lys 
225                 230                 235                 240 
Cys Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
                245                 250                 255     
Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu 
            260                 265                 270         
Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
        275                 280                 285             
Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
    290                 295                 300                 
Gly Ser Gly Gly Ser Gly Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
305                 310                 315                 320 
Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     
Glu Ala Ala Ala Lys Ala Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            340                 345                 350         
Ser Gly Gly Ser Gly Gly Ser Gly Gly Cys Gln Phe Asp Leu Ser Thr 
        355                 360                 365             
Arg Arg Leu Lys Cys Thr Ser Gly Gly Ser Xaa Gly Gly Ser Val Thr 
    370                 375                 380                 
Gly Tyr Arg Leu Phe Glu Lys Glu Ser Gly Gly Ser Gly Gly Ser Trp 
385                 390                 395                 400 
Ser His Pro Gln Phe Glu Lys 
                405         

