                         SEQUENCE LISTING

<110>  LONGAS TECHNOLOGIES PTY LTD
 
<120>  ENZYME

<130>  N411620WO

<140>  PCT/GB2019/050443
<141>  2019-02-19

<150>  GB 1802744.1
<151>  2018-02-20

<160>  142   

<170>  PatentIn version 3.5

<210>  1
<211>  2325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA polymerase from Thermococcus sp. KS-1

<400>  1
atgatcctcg acactgacta cataactgag aatggaaaac ccgtcataag gattttcaag       60

aaggagaacg gcgagtttaa gattgagtac gataggactt ttgaacccta catttacgcc      120

ctcctgaagg acgattctgc cattgaggag gtcaagaaga taaccgccga gaggcacgga      180

acggttgtaa cggttaagcg ggctgaaaag gttcagaaga agttcctcgg gagaccagtt      240

gaggtctgga aactctactt tactcaccct caggacgtcc cagcgataag ggacaagata      300

cgagagcatc cagcagttat tgacatctac gagtacgaca tacccttcgc caagcgctac      360

ctcatagaca agggattagt gccaatggaa ggcgacgagg agctgaaaat gcttgccttt      420

gatatcgaga cgctctacca tgagggcgag gagttcgccg aggggccaat ccttatgata      480

agctacgccg acgaggaagg ggccagggtg ataacgtgga agaacgcgga tctgccctac      540

gttgacgtcg tctcgacgga gagggagatg ataaagcgct tcctaaaggt ggtcaaagag      600

aaagatcctg acgtcctaat aacctacaac ggcgacaact tcgacttcgc ctacctaaaa      660

aaacgctgtg aaaagcttgg aataaacttc acgctcggaa gggacggaag cgagccgaag      720

attcagagga tgggcgacag gtttgccgtc gaagtgaagg gacggataca cttcgatctc      780

tatcctgtga taagacggac gataaacctg cccacataca cgcttgaggc cgtttatgaa      840

gccgtcttcg gtcagccgaa ggagaaggtc tacgctgagg agatagctac agcttgggag      900

agcggtgaag gccttgagag agtagccaga tactcgatgg aagatgcgaa ggtcacatac      960

gagcttggga aggagttttt ccctatggag gcccagcttt ctcgcttaat cggccagtcc     1020

ctctgggacg tctcccgctc cagcactggc aacctcgttg agtggttcct cctcaggaag     1080

gcctacgaga ggaatgagct ggccccgaac aagcccgatg aaaaggagct ggccagaaga     1140

cgacagagct atgaaggagg ctatgtaaaa gagcccgaga gagggttgtg ggagaacata     1200

gtgtacctag attttagatc tctgtacccc tcaatcatca tcacccacaa cgtctcgccg     1260

gatactctca acagggaagg atgcaaggaa tatgacgttg ccccccaggt cggtcaccgc     1320

ttctgcaagg acttcccagg atttatcccg agcctgcttg gagacctcct agaggagagg     1380

cagaagataa agaagaagat gaaggccacg attgacccga tcgagaggaa gctcctcgat     1440

tacaggcaga gggccatcaa gatcctggcc aacagctact acggttacta cggctatgca     1500

agggcgcgct ggtactgcaa ggagtgtgca gagagcgtaa cggcctgggg aagggagtac     1560

ataacgatga ccatcagaga gatagaggaa aagtacggct ttaaggtaat ctacagcgac     1620

accgacggat tttttgccac aatacctgga gccgatgctg aaaccgtcaa aaagaaggcg     1680

atggagttcc tcaagtatat caacgccaaa ctcccgggcg cgcttgagct cgagtacgag     1740

ggcttctaca aacgcggctt cttcgtcacg aagaagaagt acgcggtgat agacgaggaa     1800

ggcaagataa caacgcgcgg acttgagatt gtgaggcgcg actggagcga gatagcgaaa     1860

gagacgcagg cgagggttct tgaagctttg ctaaaggacg gtgacgtcga gaaggccgtg     1920

aggatagtca aagaagttac cgaaaagctg agcaagtacg aggttccgcc ggagaagctg     1980

gtgatccacg agcagataac gagggattta aaggactaca aggcaaccgg tccccacgtt     2040

gccgttgcca agaggttggc cgcgagagga gtcaaaatac gccctggaac ggtgataagc     2100

tacatcgtgc tcaagggctc tgggaggata ggcgacaggg cgataccgtt cgacgagttc     2160

gacccgacga agcacaagta cgacgccgag tactacattg agaaccaggt tctcccagcc     2220

gttgagagaa ttctgagagc cttcggttac cgcaaggaag acctgcgcta ccagaagacg     2280

agacaggttg gtctgggagc ctggctgaag ccgaagggaa cttga                     2325


<210>  2
<211>  774
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DNA polymerase from Thermococcus sp. KS-1

<400>  2

Met Ile Leu Asp Thr Asp Tyr Ile Thr Glu Asn Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Glu Tyr Asp Arg 
            20                  25                  30          


Thr Phe Glu Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Ala Glu Arg His Gly Thr Val Val Thr 
    50                  55                  60                  


Val Lys Arg Ala Glu Lys Val Gln Lys Lys Phe Leu Gly Arg Pro Val 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Thr His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Ile Asp Ile Tyr Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Val Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Lys Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Ala Glu Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Arg Val Ile Thr Trp Lys Asn Ala 
                165                 170                 175     


Asp Leu Pro Tyr Val Asp Val Val Ser Thr Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Lys Val Val Lys Glu Lys Asp Pro Asp Val Leu Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Ala Tyr Leu Lys Lys Arg Cys Glu 
    210                 215                 220                 


Lys Leu Gly Ile Asn Phe Thr Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Ile Gln Arg Met Gly Asp Arg Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Val Phe Gly Gln Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Glu Glu Ile Ala Thr Ala Trp Glu Ser Gly Glu Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Arg Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Ile Gly Gln Ser Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Lys Glu Leu Ala Arg Arg Arg Gln Ser Tyr 
    370                 375                 380                 


Glu Gly Gly Tyr Val Lys Glu Pro Glu Arg Gly Leu Trp Glu Asn Ile 
385                 390                 395                 400 


Val Tyr Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr His 
                405                 410                 415     


Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Lys Glu Tyr Asp 
            420                 425                 430         


Val Ala Pro Gln Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly Phe 
        435                 440                 445             


Ile Pro Ser Leu Leu Gly Asp Leu Leu Glu Glu Arg Gln Lys Ile Lys 
    450                 455                 460                 


Lys Lys Met Lys Ala Thr Ile Asp Pro Ile Glu Arg Lys Leu Leu Asp 
465                 470                 475                 480 


Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly Tyr 
                485                 490                 495     


Tyr Gly Tyr Ala Arg Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu Ser 
            500                 505                 510         


Val Thr Ala Trp Gly Arg Glu Tyr Ile Thr Met Thr Ile Arg Glu Ile 
        515                 520                 525             


Glu Glu Lys Tyr Gly Phe Lys Val Ile Tyr Ser Asp Thr Asp Gly Phe 
    530                 535                 540                 


Phe Ala Thr Ile Pro Gly Ala Asp Ala Glu Thr Val Lys Lys Lys Ala 
545                 550                 555                 560 


Met Glu Phe Leu Lys Tyr Ile Asn Ala Lys Leu Pro Gly Ala Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Val Ile Asp Glu Glu Gly Lys Ile Thr Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Arg Val Leu Glu Ala Leu Leu Lys Asp Gly Asp Val Glu Lys Ala Val 
625                 630                 635                 640 


Arg Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Val Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile His Glu Gln Ile Thr Arg Asp Leu Lys Asp 
            660                 665                 670         


Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Arg Leu Ala Ala 
        675                 680                 685             


Arg Gly Val Lys Ile Arg Pro Gly Thr Val Ile Ser Tyr Ile Val Leu 
    690                 695                 700                 


Lys Gly Ser Gly Arg Ile Gly Asp Arg Ala Ile Pro Phe Asp Glu Phe 
705                 710                 715                 720 


Asp Pro Thr Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Arg Ala Phe Gly Tyr Arg Lys 
            740                 745                 750         


Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Gly Ala Trp 
        755                 760                 765             


Leu Lys Pro Lys Gly Thr 
    770                 


<210>  3
<211>  2325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA polymerase from Thermococcus celer

<400>  3
atgatcctcg acgctgacta catcaccgaa gatgggaagc ccgtcgtgag gatattcagg       60

aaggagaagg gcgagttcag aatcgactac gacagggact tcgagcccta catctacgcc      120

ctcctgaagg acgattcggc catcgaggag gtgaagagga taaccgttga gcgccacggg      180

aaggccgtca gggttaagcg ggtggagaag gtcgaaaaga agttcctcaa caggccgata      240

gaggtctgga agctctactt caatcacccg caggacgttc cggcgataag ggacgagata      300

aggaagcatc cggccgtcgt tgatatctac gagtacgaca tccccttcgc caagcgctac      360

ctcatcgata aggggctcgt cccgatggag ggggaggagg agctcaaact gatggccttc      420

gacatcgaga ccctctacca cgagggagac gagttcgggg aggggccgat cctgatgata      480

agctacgccg acggggacgg ggcgagggtc ataacctgga agaagatcga cctcccctac      540

gtcgacgtcg tctcgaccga gaaggagatg ataaagcgct tcctccaggt ggtgaaggag      600

aaggacccgg acgtgctcgt aacttacaac ggcgacaact tcgacttcgc ctacctgaag      660

agacgctccg aggagcttgg attgaagttc atcctcggga gggacgggag cgagcccaag      720

atccagcgca tgggcgaccg cttcgccgtc gaggtgaagg ggaggataca cttcgacctc      780

tacccggtga taaggcgcac cgtgaacctg ccgacctaca cgctcgaggc ggtctacgag      840

gccatcttcg ggaggccaaa ggagaaggtc tacgccgggg agatagtgga ggcctgggaa      900

accggcgagg gtcttgagag ggttgcccgc tactccatgg aggacgcaaa ggttaccttc      960

gagctcggga gggagttctt cccgatggag gcccagctct cgaggctcat cggccagggt     1020

ctctgggacg tctcccgctc gagcaccggc aacctggtcg agtggttcct cctgaggaag     1080

gcctacgaga ggaacgaact ggccccgaac aagccgagcg gccgggaagt ggagatcagg     1140

aggcgtggct acgccggtgg ttacgttaag gagccggaga ggggtttatg ggagaacatc     1200

gtgtacctcg actttcgctc tctttacccc tccatcatca taacccacaa cgtctcgccc     1260

gataccctaa acagggaggg ctgtgagaac tacgacgtcg ccccccaggt ggggcataag     1320

ttctgcaaag attttccggg cttcatcccg agcctgctcg gaggcctgct tgaggagagg     1380

cagaagataa agcggaggat gaaggcctct gtggatcccg ttgagcggaa gctcctcgat     1440

tacaggcaga gggccatcaa gatactggcc aacagcttct acggatacta cggctacgcg     1500

agggcgaggt ggtactgcag ggagtgcgcg gagagcgtta ccgcctgggg cagggagtac     1560

atcgataggg tcatcaggga gctcgaggag aagttcggct tcaaggtgct ctacgcggac     1620

acggacggac tgcacgccac gatccccggg gcggacgccg ggaccgtcaa ggagagggcg     1680

agggggttcc tgagatacat caaccccaag ctccccggcc tcctggagct cgagtacgag     1740

gggttctacc tgaggggttt cttcgtgacg aagaagaagt acgcggtcat agacgaggag     1800

ggcaagataa ccacgcgcgg cctcgagata gtcaggcggg actggagcga ggtggccaag     1860

gagacgcagg cgagggtcct ggaggcgata ctgaggcacg gtgacgtcga ggaggccgtt     1920

agaatcgtca gggaggtaac cgaaaagctg agcaagtacg aggttccgcc ggagaaactg     1980

gtgatccacg agcagataac gagggatttg agggactaca aagccacggg accgcacgtg     2040

gcggtggcga agcgcctggc cgggaggggg gtaaggatac gccccgggac ggtgataagc     2100

tacatcgtcc tcaagggctc cggaaggata ggggacaggg cgattccctt cgacgagttc     2160

gacccgacta agcacaggta cgacgccgac tactacatcg agaaccaggt tctgccagcc     2220

gtcgagagga tcctgaaggc cttcggctac cgcaaggagg acctgaaata ccagaagacg     2280

aggcaggtgg gcctgggtgc gtggctcaac gcggggaagg ggtga                     2325


<210>  4
<211>  774
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DNA polymerase from Thermococcus celer

<400>  4

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Val Val 
1               5                   10                  15      


Arg Ile Phe Arg Lys Glu Lys Gly Glu Phe Arg Ile Asp Tyr Asp Arg 
            20                  25                  30          


Asp Phe Glu Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Glu Val Lys Arg Ile Thr Val Glu Arg His Gly Lys Ala Val Arg 
    50                  55                  60                  


Val Lys Arg Val Glu Lys Val Glu Lys Lys Phe Leu Asn Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Asn His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Glu Ile Arg Lys His Pro Ala Val Val Asp Ile Tyr Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Val Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Leu Met Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Asp Glu Phe Gly Glu Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Gly Asp Gly Ala Arg Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Asp Val Val Ser Thr Glu Lys Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Gln Val Val Lys Glu Lys Asp Pro Asp Val Leu Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Ala Tyr Leu Lys Arg Arg Ser Glu 
    210                 215                 220                 


Glu Leu Gly Leu Lys Phe Ile Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Ile Gln Arg Met Gly Asp Arg Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr Pro Val Ile Arg Arg Thr Val Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Arg Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Gly Glu Ile Val Glu Ala Trp Glu Thr Gly Glu Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Arg Tyr Ser Met Glu Asp Ala Lys Val Thr Phe 
305                 310                 315                 320 


Glu Leu Gly Arg Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Ile Gly Gln Gly Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Gly Arg Glu Val Glu Ile Arg Arg Arg Gly Tyr 
    370                 375                 380                 


Ala Gly Gly Tyr Val Lys Glu Pro Glu Arg Gly Leu Trp Glu Asn Ile 
385                 390                 395                 400 


Val Tyr Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr His 
                405                 410                 415     


Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Glu Asn Tyr Asp 
            420                 425                 430         


Val Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly Phe 
        435                 440                 445             


Ile Pro Ser Leu Leu Gly Gly Leu Leu Glu Glu Arg Gln Lys Ile Lys 
    450                 455                 460                 


Arg Arg Met Lys Ala Ser Val Asp Pro Val Glu Arg Lys Leu Leu Asp 
465                 470                 475                 480 


Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Phe Tyr Gly Tyr 
                485                 490                 495     


Tyr Gly Tyr Ala Arg Ala Arg Trp Tyr Cys Arg Glu Cys Ala Glu Ser 
            500                 505                 510         


Val Thr Ala Trp Gly Arg Glu Tyr Ile Asp Arg Val Ile Arg Glu Leu 
        515                 520                 525             


Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly Leu 
    530                 535                 540                 


His Ala Thr Ile Pro Gly Ala Asp Ala Gly Thr Val Lys Glu Arg Ala 
545                 550                 555                 560 


Arg Gly Phe Leu Arg Tyr Ile Asn Pro Lys Leu Pro Gly Leu Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Gly Phe Tyr Leu Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Val Ile Asp Glu Glu Gly Lys Ile Thr Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Val Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Arg Val Leu Glu Ala Ile Leu Arg His Gly Asp Val Glu Glu Ala Val 
625                 630                 635                 640 


Arg Ile Val Arg Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Val Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile His Glu Gln Ile Thr Arg Asp Leu Arg Asp 
            660                 665                 670         


Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Arg Leu Ala Gly 
        675                 680                 685             


Arg Gly Val Arg Ile Arg Pro Gly Thr Val Ile Ser Tyr Ile Val Leu 
    690                 695                 700                 


Lys Gly Ser Gly Arg Ile Gly Asp Arg Ala Ile Pro Phe Asp Glu Phe 
705                 710                 715                 720 


Asp Pro Thr Lys His Arg Tyr Asp Ala Asp Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Lys Ala Phe Gly Tyr Arg Lys 
            740                 745                 750         


Glu Asp Leu Lys Tyr Gln Lys Thr Arg Gln Val Gly Leu Gly Ala Trp 
        755                 760                 765             


Leu Asn Ala Gly Lys Gly 
    770                 


<210>  5
<211>  2328
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA polymerase from Thermococcus siculi

<400>  5
atgatcctcg acacggacta catcacggaa gatgggaaac ccgtcataag gatattcaag       60

aaagagaacg gcgagttcaa gatcgagtac gacaggactt ttgaacccta catctacgcc      120

ctcctgaagg acgactccgc gattgaggat gttaaaaaga taaccgccga gaggcacgga      180

acggtggtga aggtcaagcg cgccgaaaag gtgcagaaga agttcctagg caggccggtt      240

gaagtctgga agctctactt cacccacccc caagatgtcc cggcgataag ggacaagatt      300

aggaagcatc cagctgtaat tgacatctac gagtacgaca taccattcgc caagcgctac      360

ctcatcgaca agggcctgat tccgatggag ggtgaagaag agcttaagat gctcgccttc      420

gacattgaga cgctctacca tgagggtgag gagttcgccg aggggcctat tctgatgata      480

agctacgccg acgagagcga ggcacgcgtc atcacctgga agaaaatcga cctcccctac      540

gttgacgtcg tctcaacgga gaaggagatg ataaagcgct tcctccgcgt tgtgaaggag      600

aaagatcccg atgtcctcat aacctacaac ggcgacaact tcgacttcgc ctacctgaag      660

aagcgctgtg aaaagcttgg aataaacttc ctccttggaa gggacgggag cgagccgaag      720

atccagagaa tgggtgaccg cttcgccgtt gaggtgaagg ggaggataca cttcgacctc      780

tatcctgtaa taaggcgcac gataaacctg ccgacctaca tgcttgaggc agtctacgag      840

gccatctttg ggaagccaaa ggagaaggtt tacgccgagg agatagccac cgcttgggaa      900

accggagagg gccttgagag ggtggctcgc tactctatgg aggacgcgaa ggtcacgttt      960

gagcttggaa aggagttctt cccgatggag gcccaacttt cgaggttggt cggccagagc     1020

ttctgggatg tcgcgcgctc aagcacgggc aatctggtcg agtggttcct cctcaggaag     1080

gcctacgaga ggaacgagct ggctccaaac aagccctctg gaagggaata tgacgagagg     1140

cgcggtggat acgccggcgg ctacgtcaag gaaccggaaa agggcctgtg ggagaacata     1200

gtctacctcg actataaatc tctctacccc tcaatcatca tcacccacaa cgtctcgccc     1260

gataccctca accgcgaggg ctgtaaggag tatgacgtag ctccacaggt cggccaccgc     1320

ttctgcaagg actttccagg cttcatcccg agcctgctcg gggatctcct ggaggagagg     1380

cagaagataa agaggaagat gaaggcaaca attgacccga tcgagagaaa gctccttgat     1440

tacaggcaac gggccatcaa gatccttcta aatagttttt acggctacta cggctacgca     1500

agggctcgct ggtactgcaa ggagtgtgcc gagagcgtta cggcatgggg aagggaatat     1560

atcaccatga caatcaggga aatagaagag aagtatggct ttaaagtact ttatgcggac     1620

actgacggct tcttcgcgac gattcccggg gaagatgccg agaccatcaa aaagagggcg     1680

atggagttcc tcaagtacat aaacgccaaa ctccccggtg cgctcgaact tgagtacgag     1740

gacttctaca ggcgcggctt cttcgtcacc aagaagaaat acgcggttat cgacgaggag     1800

ggcaagataa caacgcgcgg gctggagatc gtcaggcgcg actggagcga gatagccaag     1860

gagacgcagg cgcgggttct ggaggccctt ctgaaggacg gtgacgtcga agaggccgtg     1920

agcatagtca aagaagtgac cgagaagctg agcaagtacg aggttccgcc ggagaagctc     1980

gttatccacg agcagataac gcgcgagctg aaggactaca aggcaacggg accacacgtg     2040

gcgatagcga agaggttagc cgcgagaggc gtcaaaatcc gccccgggac agtcatcagc     2100

tacatcgtgc tcaagggctc cgggaggata ggcgacaggg cgattccctt cgacgagttc     2160

gaccccacga agcacaagta cgatgcagag tactacatcg agaaccaggt tctacctgcc     2220

gtcgagagga ttctgaaggc cttcggctat cgcggtgagg agctcagata ccagaagacg     2280

aggcaggttg gacttggggc gtggctgaag ccgaagggga aggggtga                  2328


<210>  6
<211>  775
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DNA polymerase from Thermococcus siculi

<400>  6

Met Ile Leu Asp Thr Asp Tyr Ile Thr Glu Asp Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Glu Tyr Asp Arg 
            20                  25                  30          


Thr Phe Glu Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Asp Val Lys Lys Ile Thr Ala Glu Arg His Gly Thr Val Val Lys 
    50                  55                  60                  


Val Lys Arg Ala Glu Lys Val Gln Lys Lys Phe Leu Gly Arg Pro Val 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Thr His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Lys His Pro Ala Val Ile Asp Ile Tyr Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Ala Glu Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Ser Glu Ala Arg Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Asp Val Val Ser Thr Glu Lys Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Val Val Lys Glu Lys Asp Pro Asp Val Leu Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Ala Tyr Leu Lys Lys Arg Cys Glu 
    210                 215                 220                 


Lys Leu Gly Ile Asn Phe Leu Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Ile Gln Arg Met Gly Asp Arg Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Met Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Glu Glu Ile Ala Thr Ala Trp Glu Thr Gly Glu Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Arg Tyr Ser Met Glu Asp Ala Lys Val Thr Phe 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Ser Phe Trp Asp Val Ala Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Gly Arg Glu Tyr Asp Glu Arg Arg Gly Gly Tyr 
    370                 375                 380                 


Ala Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn Ile 
385                 390                 395                 400 


Val Tyr Leu Asp Tyr Lys Ser Leu Tyr Pro Ser Ile Ile Ile Thr His 
                405                 410                 415     


Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Lys Glu Tyr Asp 
            420                 425                 430         


Val Ala Pro Gln Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly Phe 
        435                 440                 445             


Ile Pro Ser Leu Leu Gly Asp Leu Leu Glu Glu Arg Gln Lys Ile Lys 
    450                 455                 460                 


Arg Lys Met Lys Ala Thr Ile Asp Pro Ile Glu Arg Lys Leu Leu Asp 
465                 470                 475                 480 


Tyr Arg Gln Arg Ala Ile Lys Ile Leu Leu Asn Ser Phe Tyr Gly Tyr 
                485                 490                 495     


Tyr Gly Tyr Ala Arg Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu Ser 
            500                 505                 510         


Val Thr Ala Trp Gly Arg Glu Tyr Ile Thr Met Thr Ile Arg Glu Ile 
        515                 520                 525             


Glu Glu Lys Tyr Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly Phe 
    530                 535                 540                 


Phe Ala Thr Ile Pro Gly Glu Asp Ala Glu Thr Ile Lys Lys Arg Ala 
545                 550                 555                 560 


Met Glu Phe Leu Lys Tyr Ile Asn Ala Lys Leu Pro Gly Ala Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Asp Phe Tyr Arg Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Val Ile Asp Glu Glu Gly Lys Ile Thr Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Arg Val Leu Glu Ala Leu Leu Lys Asp Gly Asp Val Glu Glu Ala Val 
625                 630                 635                 640 


Ser Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Val Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile His Glu Gln Ile Thr Arg Glu Leu Lys Asp 
            660                 665                 670         


Tyr Lys Ala Thr Gly Pro His Val Ala Ile Ala Lys Arg Leu Ala Ala 
        675                 680                 685             


Arg Gly Val Lys Ile Arg Pro Gly Thr Val Ile Ser Tyr Ile Val Leu 
    690                 695                 700                 


Lys Gly Ser Gly Arg Ile Gly Asp Arg Ala Ile Pro Phe Asp Glu Phe 
705                 710                 715                 720 


Asp Pro Thr Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Lys Ala Phe Gly Tyr Arg Gly 
            740                 745                 750         


Glu Glu Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Gly Ala Trp 
        755                 760                 765             


Leu Lys Pro Lys Gly Lys Gly 
    770                 775 


<210>  7
<211>  774
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DNA polymerase from Thermococcus kodakarensis

<400>  7

Met Ile Leu Asp Thr Asp Tyr Ile Thr Glu Asp Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Glu Tyr Asp Arg 
            20                  25                  30          


Thr Phe Glu Pro Tyr Phe Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Ala Glu Arg His Gly Thr Val Val Thr 
    50                  55                  60                  


Val Lys Arg Val Glu Lys Val Gln Lys Lys Phe Leu Gly Arg Pro Val 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Thr His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Ile Asp Ile Tyr Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Val Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Lys Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr Glu Glu Gly Glu Glu Phe Ala Glu Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Arg Val Ile Thr Trp Lys Asn Val 
                165                 170                 175     


Asp Leu Pro Tyr Val Asp Val Val Ser Thr Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Val Val Lys Glu Lys Asp Pro Asp Val Leu Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Ala Tyr Leu Lys Lys Arg Cys Glu 
    210                 215                 220                 


Lys Leu Gly Ile Asn Phe Ala Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Ile Gln Arg Met Gly Asp Arg Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Val Phe Gly Gln Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Glu Glu Ile Thr Thr Ala Trp Glu Thr Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Arg Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Ile Gly Gln Ser Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Lys Glu Leu Ala Arg Arg Arg Gln Ser Tyr 
    370                 375                 380                 


Glu Gly Gly Tyr Val Lys Glu Pro Glu Arg Gly Leu Trp Glu Asn Ile 
385                 390                 395                 400 


Val Tyr Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr His 
                405                 410                 415     


Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Lys Glu Tyr Asp 
            420                 425                 430         


Val Ala Pro Gln Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly Phe 
        435                 440                 445             


Ile Pro Ser Leu Leu Gly Asp Leu Leu Glu Glu Arg Gln Lys Ile Lys 
    450                 455                 460                 


Lys Lys Met Lys Ala Thr Ile Asp Pro Ile Glu Arg Lys Leu Leu Asp 
465                 470                 475                 480 


Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly Tyr 
                485                 490                 495     


Tyr Gly Tyr Ala Arg Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu Ser 
            500                 505                 510         


Val Thr Ala Trp Gly Arg Glu Tyr Ile Thr Met Thr Ile Lys Glu Ile 
        515                 520                 525             


Glu Glu Lys Tyr Gly Phe Lys Val Ile Tyr Ser Asp Thr Asp Gly Phe 
    530                 535                 540                 


Phe Ala Thr Ile Pro Gly Ala Asp Ala Glu Thr Val Lys Lys Lys Ala 
545                 550                 555                 560 


Met Glu Phe Leu Lys Tyr Ile Asn Ala Lys Leu Pro Gly Ala Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Gly Phe Tyr Glu Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Val Ile Asp Glu Glu Gly Lys Ile Thr Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Arg Val Leu Glu Ala Leu Leu Lys Asp Gly Asp Val Glu Lys Ala Val 
625                 630                 635                 640 


Arg Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Val Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile His Glu Gln Ile Thr Arg Asp Leu Lys Asp 
            660                 665                 670         


Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Arg Leu Ala Ala 
        675                 680                 685             


Arg Gly Val Lys Ile Arg Pro Gly Thr Val Ile Ser Tyr Ile Val Leu 
    690                 695                 700                 


Lys Gly Ser Gly Arg Ile Gly Asp Arg Ala Ile Pro Phe Asp Glu Phe 
705                 710                 715                 720 


Asp Pro Thr Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Arg Ala Phe Gly Tyr Arg Lys 
            740                 745                 750         


Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Ser Ala Trp 
        755                 760                 765             


Leu Lys Pro Lys Gly Thr 
    770                 


<210>  8
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  8
tagaattgaa gaa                                                          13


<210>  9
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  9
tggccatagc tac                                                          13


<210>  10
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  10
gtcatctgcg acc                                                          13


<210>  11
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  11
ttcgcgcttg gac                                                          13


<210>  12
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  12
cgcgaaccgt tag                                                          13


<210>  13
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  13
ttgcagcctc taa                                                          13


<210>  14
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  14
tctactagta cga                                                          13


<210>  15
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  15
gtaggttcta ctg                                                          13


<210>  16
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  16
gccaatatca agt                                                          13


<210>  17
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  17
ctatcttgct ggt                                                          13


<210>  18
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  18
gttctcatag gta                                                          13


<210>  19
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  19
gtctatgaac caa                                                          13


<210>  20
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  20
cggagcgctt att                                                          13


<210>  21
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  21
tatgccatga gga                                                          13


<210>  22
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  22
atacgactcg gag                                                          13


<210>  23
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  23
gatggaactc agc                                                          13


<210>  24
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  24
ggacctgcat gaa                                                          13


<210>  25
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  25
tagactggaa ctt                                                          13


<210>  26
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  26
gaattacctc gtt                                                          13


<210>  27
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  27
aggatcaggc tac                                                          13


<210>  28
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  28
acgcgtagaa gag                                                          13


<210>  29
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  29
cttcgagact tac                                                          13


<210>  30
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  30
gacggctaac tcc                                                          13


<210>  31
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  31
ttagcattct ctt                                                          13


<210>  32
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  32
gcaaggcata gta                                                          13


<210>  33
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  33
acctagatat gga                                                          13


<210>  34
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  34
acgccaaggc gta                                                          13


<210>  35
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  35
tatgacggat ccg                                                          13


<210>  36
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  36
cctccattag aga                                                          13


<210>  37
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  37
attgaatact ctg                                                          13


<210>  38
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  38
gagatgagaa gaa                                                          13


<210>  39
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  39
tctgagtagc cgg                                                          13


<210>  40
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  40
aataggtagt acg                                                          13


<210>  41
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  41
gtcgaagaag tcc                                                          13


<210>  42
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  42
tactgcatct cgt                                                          13


<210>  43
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  43
gacgtattag agc                                                          13


<210>  44
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  44
cctgcattat tcg                                                          13


<210>  45
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  45
acgaatgatg ctc                                                          13


<210>  46
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  46
tactagcaga gat                                                          13


<210>  47
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  47
ctcctcatct tcc                                                          13


<210>  48
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  48
tcctctgcgc tgc                                                          13


<210>  49
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  49
ccttctcagt ccg                                                          13


<210>  50
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  50
cagcttcata gcg                                                          13


<210>  51
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  51
ttgactctcg cgc                                                          13


<210>  52
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  52
tatcctgagc gat                                                          13


<210>  53
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  53
aacgcctagc cga                                                          13


<210>  54
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  54
ccgaagacgt cat                                                          13


<210>  55
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  55
gagttctcca gat                                                          13


<210>  56
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  56
tgcatccgcg ctt                                                          13


<210>  57
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  57
cctgaactca agt                                                          13


<210>  58
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sample tag sequence

<400>  58
ggtcgtatgc gta                                                          13


<210>  59
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  59
aggcctctct acc                                                          13


<210>  60
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  60
gtactccatc caa                                                          13


<210>  61
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  61
cagcggacgc gct                                                          13


<210>  62
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  62
atctctctta gca                                                          13


<210>  63
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  63
aagcaataat aat                                                          13


<210>  64
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  64
aaggcgactc cga                                                          13


<210>  65
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  65
acgtctctag gag                                                          13


<210>  66
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  66
ccatcagacc tct                                                          13


<210>  67
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  67
acttaatcgt act                                                          13


<210>  68
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  68
tggaattctc caa                                                          13


<210>  69
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  69
ccatacgatc agg                                                          13


<210>  70
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  70
ttatggagca ata                                                          13


<210>  71
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  71
gctcggcgtt cga                                                          13


<210>  72
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  72
ttggccagtc gct                                                          13


<210>  73
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  73
cagatacgta gag                                                          13


<210>  74
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  74
aatgctatta tcc                                                          13


<210>  75
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  75
gcagcatgcc gat                                                          13


<210>  76
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  76
ggagagttac ctc                                                          13


<210>  77
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  77
gagagtccat gat                                                          13


<210>  78
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  78
caatctattc tga                                                          13


<210>  79
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  79
gctcttagta tcc                                                          13


<210>  80
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  80
ccatagttat ggt                                                          13


<210>  81
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  81
tgcgagatcg aag                                                          13


<210>  82
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  82
agagaagtcg agt                                                          13


<210>  83
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  83
ggtaactcca tat                                                          13


<210>  84
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  84
tgctattcca ggc                                                          13


<210>  85
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  85
aaccgcgagg ctc                                                          13


<210>  86
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  86
ttctagagat acc                                                          13


<210>  87
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  87
ttcgctcaag tat                                                          13


<210>  88
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  88
cagagaaggc gca                                                          13


<210>  89
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  89
tagaattggc ctc                                                          13


<210>  90
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  90
ggccattctc cag                                                          13


<210>  91
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  91
tccaacgcgc gtt                                                          13


<210>  92
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  92
gccgcagatt acg                                                          13


<210>  93
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  93
gcagttcgaa cgc                                                          13


<210>  94
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  94
ttctctctgc agg                                                          13


<210>  95
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  95
taagctacca gcg                                                          13


<210>  96
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  96
ctgcatgagg ttg                                                          13


<210>  97
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  97
ttgcctagcg agg                                                          13


<210>  98
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  98
caactgaatt agg                                                          13


<210>  99
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  99
aagcggtcct ctt                                                          13


<210>  100
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  100
aatggaagga ccg                                                          13


<210>  101
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  101
gagttagtaa gtt                                                          13


<210>  102
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  102
ttcctaattc caa                                                          13


<210>  103
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  103
gttctggttc gct                                                          13


<210>  104
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  104
gttcatctct tcc                                                          13


<210>  105
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  105
attccgagga aga                                                          13


<210>  106
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  106
cttagccgag aga                                                          13


<210>  107
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  107
gtctgctacg ctt                                                          13


<210>  108
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  108
atggcgccgc gca                                                          13


<210>  109
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  109
taattggtta tct                                                          13


<210>  110
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  110
tcggttataa gtc                                                          13


<210>  111
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  111
tgcctgagaa cgt                                                          13


<210>  112
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  112
agatgcggtt aac                                                          13


<210>  113
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  113
atggaatagg cga                                                          13


<210>  114
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  114
agagatgcga tcg                                                          13


<210>  115
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  115
ctccaactaa cgt                                                          13


<210>  116
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  116
gccttgctac tgg                                                          13


<210>  117
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  117
cttcgtctct acg                                                          13


<210>  118
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  118
acgctcatag cct                                                          13


<210>  119
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  119
gtcgaagata agg                                                          13


<210>  120
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  120
gccggagtcc tcg                                                          13


<210>  121
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  121
tatacggcga cct                                                          13


<210>  122
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  122
aggtagatat tcg                                                          13


<210>  123
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  123
ttaaggtact gct                                                          13


<210>  124
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  124
cggatctggt ata                                                          13


<210>  125
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  125
gaggtctcgg agg                                                          13


<210>  126
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  126
ggcatcgatg gac                                                          13


<210>  127
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  127
gatctccgat ata                                                          13


<210>  128
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  128
gattcggaat act                                                          13


<210>  129
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  129
ctgcgatccg gcc                                                          13


<210>  130
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  130
gatccggttg caa                                                          13


<210>  131
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  131
cgtcaggctt gac                                                          13


<210>  132
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  132
tcggcaaggc gag                                                          13


<210>  133
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  133
gaacggcgaa cgc                                                          13


<210>  134
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  134
cctcaagcgg act                                                          13


<210>  135
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  135
gaagccagat ggt                                                          13


<210>  136
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sample tag sequence

<400>  136
tgctcatacc aat                                                          13


<210>  137
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i7 custom index primer (Table 2)


<220>
<221>  misc_feature
<222>  (25)..(36)
<223>  n is a, c, g, or t

<400>  137
caagcagaag acggcatacg agatnnnnnn nnnnnngtct cgtgggctcg g                51


<210>  138
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i5 custom index primer (Table 2)


<220>
<221>  misc_feature
<222>  (30)..(41)
<223>  n is a, c, g, or t

<400>  138
aatgatacgg cgaccaccga gatctacacn nnnnnnnnnn ntcgtcggca gcgtc            55


<210>  139
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i7 flow cell primer (Table 3)

<400>  139
caagcagaag acggcatacg a                                                 21


<210>  140
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  i5 flow cell primer (Table 3)

<400>  140
aatgatacgg cgaccaccga                                                   20


<210>  141
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single_mut for mutagenesis (Table 5)


<220>
<221>  misc_feature
<222>  (19)..(34)
<223>  n is a, c, g, or t

<400>  141
tcggtctgcg cctctagcnn nnnnnnnnnn nnnngtctcg tgggctcgga g                51


<210>  142
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single_rec primer for recovery (Table 5)

<400>  142
caagcagaag acggcatacg agattcggtc tgcgcctcta gc                          42


