                         SEQUENCE LISTING

<110>  Stenmark, Pl
 
<120>  TricepTox

<130>  P41705056PCT00

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hc/TriRecABTox

<400>  1

Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn 
1               5                   10                  15      


His Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser 
            20                  25                  30          


Lys Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn 
        35                  40                  45              


Leu Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr 
    50                  55                  60                  


Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro 
65                  70                  75                  80  


Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn 
                85                  90                  95      


Cys Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu 
            100                 105                 110         


Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val 
        115                 120                 125             


Phe Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp 
    130                 135                 140                 


Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr 
145                 150                 155                 160 


Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn 
                165                 170                 175     


Ile His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp 
            180                 185                 190         


Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu 
        195                 200                 205             


Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser 
    210                 215                 220                 


Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro 
225                 230                 235                 240 


Tyr Tyr Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys 
                245                 250                 255     


Lys Asp Ser Pro Val Gly Glu Ile Leu Gly Pro Arg Gly Ser Val Met 
            260                 265                 270         


Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Glu Lys Phe 
        275                 280                 285             


Ile Ile Arg Arg Lys Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val 
    290                 295                 300                 


Arg Asn Glu Asp Tyr Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu 
305                 310                 315                 320 


Trp Arg Val Tyr Thr Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu 
                325                 330                 335     


Phe Leu Ala Pro Ile Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln 
            340                 345                 350         


Ile Lys Glu Tyr Asp Glu Gln Gly Thr Asn Ser Cys Gln Leu Leu Phe 
        355                 360                 365             


Lys Lys Asp Glu Glu Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His 
    370                 375                 380                 


Arg Phe Tyr Glu Ser Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe 
385                 390                 395                 400 


Cys Ile Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn 
                405                 410                 415     


Leu Lys Leu Gly Cys Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp 
            420                 425                 430         


Gly Glu Arg Pro Leu 
        435         


<210>  2
<211>  1314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hc/TriRecABTox codon optimised sequence for expression in E. coli

<400>  2
aagaacatta tcaacaccag catcctgaac ctgcgctacg agagcaacca cctgatcgac       60

ctgagccgct acgcgagcaa gattaacatc ggtagcaagg tgaactttga cccgattgat      120

aaaaaccaga tccaactgtt caacctggaa agcagcaaga tcgaagtgat tctgaaaaac      180

gcgattgttt ataacagcat gtacgaaaac ttcagcacca gcttttggat ccgtattccg      240

aagtatttta acagcatcag cctgaacaac gaatacacca tcattaactg catggagaac      300

aacagcggtt ggaaagtgag cctgaactac ggcgaaatca tttggaccct gcaggacacc      360

caagagatca agcagcgtgt ggttttcaag tacagccaaa tgatcaacat cagcgattac      420

atcaaccgtt ggattttcgt taccatcacc aacaaccgtc tgaacaacag caagatctac      480

attaacggtc gtctgattga ccagaaaccg atcagcaacc tgggcaacat tcacgcgagc      540

aacaacatca tgttcaagct ggacggttgc cgtgataccc accgttatat ctggattaag      600

tacttcaacc tgtttgataa agagctgaac gaaaaggaga ttaaagacct gtatgataac      660

cagagcaaca gcggtatcct gaaggacttt tggggcgatt atctgcaata cgacaaaccg      720

tactatatgt tcaacgcggg taacaagaac agctacatta aactgaagaa agatagcccg      780

gtgggtgaaa tcctgggtcc gcgtggcagc gttatgacca ccaacatcta tctgaacagc      840

agcctgtacc gtggcgagaa gttcatcatt cgtcgtaaaa gcaacagcca gagcattaac      900

gacgatatcg tgcgtaacga agactacatt tatctggatt tctttaacct gaaccaagag      960

tggcgtgttt acacctacaa gtacttcaag aaagaggaag agaagctgtt cctggcgccg     1020

atcagcgaca gcgatgaatt ctacaacacc atccaaatca aggaatacga cgagcagggt     1080

accaacagct gccaactgct gttcaagaaa gacgaagaga gcaccgatga aatcggtctg     1140

atcggcattc accgtttcta cgagagcggc atcgtgttcg aagagtacaa ggattacttc     1200

tgcatcagca agtggtatct gaaagaggtt aagcgtaaac cgtacaacct gaaactgggc     1260

tgcaactggc aatttattcc ggtggatgat ggctggggtg aacgtccgct gtaa           1314


<210>  3
<211>  1311
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Full-length inactive TriRecABTox with engineered activation site

<400>  3

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Gln 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Ala Lys Thr Phe Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Ile Glu Gly Arg Asp Lys 
        435                 440                 445             


Gly Tyr Asn Lys Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp 
    450                 455                 460                 


Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn 
465                 470                 475                 480 


Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu 
                485                 490                 495     


Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe 
            500                 505                 510         


Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile 
        515                 520                 525             


Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly 
    530                 535                 540                 


Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala 
545                 550                 555                 560 


Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val 
                565                 570                 575     


Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser 
            580                 585                 590         


Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu 
        595                 600                 605             


Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu 
    610                 615                 620                 


Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr 
625                 630                 635                 640 


Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe 
                645                 650                 655     


Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile 
            660                 665                 670         


Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr 
        675                 680                 685             


Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser 
    690                 695                 700                 


Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn 
705                 710                 715                 720 


Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met 
                725                 730                 735     


Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn 
            740                 745                 750         


Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe 
        755                 760                 765             


Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala 
    770                 775                 780                 


Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu 
785                 790                 795                 800 


Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp 
                805                 810                 815     


Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly 
            820                 825                 830         


Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr 
        835                 840                 845             


Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln 
    850                 855                 860                 


Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr 
865                 870                 875                 880 


Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser 
                885                 890                 895     


Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro 
            900                 905                 910         


Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile 
        915                 920                 925             


Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn 
    930                 935                 940                 


Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile 
945                 950                 955                 960 


Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser 
                965                 970                 975     


Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln 
            980                 985                 990         


Asp Thr Gln Glu Ile Lys Gln Arg  Val Val Phe Lys Tyr  Ser Gln Met 
        995                 1000                 1005             


Ile Asn  Ile Ser Asp Tyr Ile  Asn Arg Trp Ile Phe  Val Thr Ile 
    1010                 1015                 1020             


Thr Asn  Asn Arg Leu Asn Asn  Ser Lys Ile Tyr Ile  Asn Gly Arg 
    1025                 1030                 1035             


Leu Ile  Asp Gln Lys Pro Ile  Ser Asn Leu Gly Asn  Ile His Ala 
    1040                 1045                 1050             


Ser Asn  Asn Ile Met Phe Lys  Leu Asp Gly Cys Arg  Asp Thr His 
    1055                 1060                 1065             


Arg Tyr  Ile Trp Ile Lys Tyr  Phe Asn Leu Phe Asp  Lys Glu Leu 
    1070                 1075                 1080             


Asn Glu  Lys Glu Ile Lys Asp  Leu Tyr Asp Asn Gln  Ser Asn Ser 
    1085                 1090                 1095             


Gly Ile  Leu Lys Asp Phe Trp  Gly Asp Tyr Leu Gln  Tyr Asp Lys 
    1100                 1105                 1110             


Pro Tyr  Tyr Met Phe Asn Ala  Gly Asn Lys Asn Ser  Tyr Ile Lys 
    1115                 1120                 1125             


Leu Lys  Lys Asp Ser Pro Val  Gly Glu Ile Leu Gly  Pro Arg Gly 
    1130                 1135                 1140             


Ser Val  Met Thr Thr Asn Ile  Tyr Leu Asn Ser Ser  Leu Tyr Arg 
    1145                 1150                 1155             


Gly Glu  Lys Phe Ile Ile Arg  Arg Lys Ser Asn Ser  Gln Ser Ile 
    1160                 1165                 1170             


Asn Asp  Asp Ile Val Arg Asn  Glu Asp Tyr Ile Tyr  Leu Asp Phe 
    1175                 1180                 1185             


Phe Asn  Leu Asn Gln Glu Trp  Arg Val Tyr Thr Tyr  Lys Tyr Phe 
    1190                 1195                 1200             


Lys Lys  Glu Glu Glu Lys Leu  Phe Leu Ala Pro Ile  Ser Asp Ser 
    1205                 1210                 1215             


Asp Glu  Phe Tyr Asn Thr Ile  Gln Ile Lys Glu Tyr  Asp Glu Gln 
    1220                 1225                 1230             


Gly Thr  Asn Ser Cys Gln Leu  Leu Phe Lys Lys Asp  Glu Glu Ser 
    1235                 1240                 1245             


Thr Asp  Glu Ile Gly Leu Ile  Gly Ile His Arg Phe  Tyr Glu Ser 
    1250                 1255                 1260             


Gly Ile  Val Phe Glu Glu Tyr  Lys Asp Tyr Phe Cys  Ile Ser Lys 
    1265                 1270                 1275             


Trp Tyr  Leu Lys Glu Val Lys  Arg Lys Pro Tyr Asn  Leu Lys Leu 
    1280                 1285                 1290             


Gly Cys  Asn Trp Gln Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1295                 1300                 1305             


Arg Pro  Leu 
    1310     


<210>  4
<211>  3936
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full-length inactive TriRecABTox w engineered activation site, 
       codon opimised sequence for expression in E. coli

<400>  4
atgccgttcg tgaataagca gttcaactac aaagatccgg ttaatggcgt ggacatcgcg       60

tacatcaaaa tcccgaatgc gggtcagatg cagccggtga aggcgttcaa aatccacaac      120

aaaatttggg ttatcccgga gcgtgacacc tttaccaacc cggaggaagg tgatctgaac      180

ccgccgccgg aagcgaaaca agtgccggtt agctactatg acagcaccta tctgagcacc      240

gacaacgaga aggataacta cctgaagggc gtgaccaaac tgttcgaacg tatctacagc      300

accgatctgg gtcgtatgct gctgaccagc attgttcgtg gcatcccgtt ttggggtggc      360

agcaccatcg acaccgaact gaaagtgatt gataccaact gcattaacgt tatccagccg      420

gatggtagct accgtagcga ggaactgaac ctggtgatca ttggcccgag cgcggacatc      480

attcagtttg agtgcaagag cttcggtcac gaagttctga acctgacccg taacggttac      540

ggcagcaccc aatatatccg tttcagcccg gatttcacct ttggcttcga ggaaagcctg      600

gaagtggaca ccaacccgct gctgggtgcg ggcaagtttg cgaccgaccc ggcggttacc      660

ctggcgcacc agctgatcca tgcgggtcac cgtctgtacg gcattgcgat caacccgaac      720

cgtgtgttca aagttaacac caacgcgtac tatgagatga gcggtctgga agtgagcttt      780

gaggaactgc gtaccttcgg tggccacgac gcgaagttta tcgatagcct gcaggagaac      840

gaattccgtc tgtactacta caacaagttc aaggacatcg cgagcaccct gaacaaggcg      900

aaaagcattg tgggtaccac cgcgagcctg caatacatga agaacgtttt caaggagaag      960

tacctgctga gcgaagatac cagcggcaag tttagcgtgg acaagctgaa attcgataag     1020

ctgtataaaa tgctgaccga gatctacacc gaagataact tcgtgaagtt ctttaaagtt     1080

ctgaacgcga aaacctttct gaacttcgac aaggcggttt ttaaaattaa catcgtgccg     1140

aaggttaact acaccatcta tgatggtttc aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggcc agaacaccga gattaacaac atgaacttta ccaagctgaa aaacttcacc     1260

ggtctgtttg aattctataa actgctgtgc gtgcgtggca tcattaccag caagaccaaa     1320

agcctgatcg aaggtcgtga caagggctac aacaaagcgc tgaacgatct gtgcattaaa     1380

gttaacaact gggacctgtt ctttagcccg agcgaggaca acttcaccaa cgatctgaac     1440

aagggcgagg aaatcaccag cgacaccaac attgaagcgg cggaggaaaa catcagcctg     1500

gatctgattc agcaatatta cctgaccttt aacttcgaca acgagccgga aaacattagc     1560

atcgagaacc tgagcagcga catcattggt cagctggagc tgatgccgaa catcgaacgt     1620

ttcccgaacg gcaagaaata cgaactggat aaatatacca tgttccacta cctgcgtgcg     1680

caagagtttg aacacggcaa gagccgtatt gcgctgacca acagcgtgaa cgaggcgctg     1740

ctgaacccga gccgtgttta taccttcttt agcagcgact acgtgaagaa agttaacaaa     1800

gcgaccgagg cggcgatgtt cctgggttgg gtggaacagc tggtttacga ctttaccgat     1860

gaaaccagcg aggtgagcac caccgacaaa attgcggata tcaccatcat tatcccgtat     1920

atcggtccgg cgctgaacat tggcaacatg ctgtacaagg acgattttgt gggtgcgctg     1980

atcttcagcg gcgcggttat cctgctggag ttcattccgg aaattgcgat cccggtgctg     2040

ggtacctttg cgctggttag ctacatcgcg aacaaggtgc tgaccgttca aaccattgat     2100

aacgcgctga gcaagcgtaa cgagaaatgg gacgaagtgt ataaatacat cgttaccaac     2160

tggctggcga aggttaacac ccagattgac ctgatccgta agaaaatgaa agaggcgctg     2220

gaaaaccaag cggaggcgac caaggcgatt atcaactatc agtacaacca atacaccgag     2280

gaagagaaaa acaacattaa cttcaacatc gacgatctga gcagcaagct gaacgaaagc     2340

atcaacaaag cgatgattaa catcaacaag tttctgaacc agtgcagcgt gagctatctg     2400

atgaacagca tgattccgta cggtgttaag cgtctggagg acttcgatgc gagcctgaag     2460

gacgcgctgc tgaaatatat ctacgataac cgtggtaccc tgattggcca agtggaccgt     2520

ctgaaggata aagttaacaa caccctgagc accgatatcc cgttccagct gagcaaatat     2580

gtggacaacc aacgtctgct gagcaccttt accgagtaca tcaagaacat tatcaacacc     2640

agcattctga acctgcgtta tgaaagcaac cacctgatcg acctgagccg ttacgcgagc     2700

aagattaaca tcggtagcaa agttaacttc gacccgatcg ataaaaacca gattcaactg     2760

tttaacctgg agagcagcaa gattgaagtg atcctgaaaa acgcgatcgt ttacaacagc     2820

atgtatgaga actttagcac cagcttctgg attcgtatcc cgaaatattt caacagcatt     2880

agcctgaaca acgagtacac cattatcaac tgcatggaaa acaacagcgg ttggaaggtg     2940

agcctgaact acggcgagat tatctggacc ctgcaggaca cccaagaaat caagcagcgt     3000

gtggttttca agtacagcca aatgatcaac atcagcgatt acattaaccg ttggatcttt     3060

gttaccatta ccaacaaccg tctgaacaac agcaaaattt acatcaacgg tcgtctgatc     3120

gaccagaagc cgattagcaa cctgggcaac atccacgcga gcaacaacat tatgttcaag     3180

ctggacggtt gccgtgatac ccaccgttat atttggatca agtacttcaa cctgttcgat     3240

aaggagctga acgagaagga aatcaaagac ctgtatgata accagagcaa cagcggtatt     3300

ctgaaagact tctggggcga ttacctgcaa tatgacaagc cgtattacat gtttaacgcg     3360

ggtaacaaga acagctacat caaactgaag aaagatagcc cggtgggtga aattctgggt     3420

ccgcgtggca gcgttatgac caccaacatc tatctgaaca gcagcctgta ccgtggcgaa     3480

aagttcatta tccgtcgtaa aagcaacagc cagagcatca acgacgatat tgtgcgtaac     3540

gaggactata tctacctgga tttctttaac ctgaaccaag aatggcgtgt ttacacctac     3600

aagtacttca agaaagaaga ggaaaagctg tttctggcgc cgattagcga cagcgatgaa     3660

ttctataaca ccattcagat caaagagtac gacgaacagg gtaccaacag ctgccaactg     3720

ctgtttaaga aagacgagga aagcaccgat gagatcggtc tgattggcat ccaccgtttt     3780

tacgaaagcg gcatcgtgtt cgaggaatac aaggattact tctgcatcag caagtggtat     3840

ctgaaagagg ttaagcgtaa accgtacaac ctgaaactgg gctgcaactg gcaatttatt     3900

ccggtggatg atggctgggg tgaacgtccg ctgtaa                               3936


<210>  5
<211>  1311
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Full-length active TreRecABTox with engineered activation site

<400>  5

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Ile Glu Gly Arg Asp Lys 
        435                 440                 445             


Gly Tyr Asn Lys Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp 
    450                 455                 460                 


Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn 
465                 470                 475                 480 


Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu 
                485                 490                 495     


Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe 
            500                 505                 510         


Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile 
        515                 520                 525             


Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly 
    530                 535                 540                 


Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala 
545                 550                 555                 560 


Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val 
                565                 570                 575     


Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser 
            580                 585                 590         


Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu 
        595                 600                 605             


Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu 
    610                 615                 620                 


Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr 
625                 630                 635                 640 


Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe 
                645                 650                 655     


Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile 
            660                 665                 670         


Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr 
        675                 680                 685             


Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser 
    690                 695                 700                 


Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn 
705                 710                 715                 720 


Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met 
                725                 730                 735     


Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn 
            740                 745                 750         


Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe 
        755                 760                 765             


Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala 
    770                 775                 780                 


Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu 
785                 790                 795                 800 


Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp 
                805                 810                 815     


Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly 
            820                 825                 830         


Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr 
        835                 840                 845             


Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln 
    850                 855                 860                 


Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr 
865                 870                 875                 880 


Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser 
                885                 890                 895     


Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro 
            900                 905                 910         


Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile 
        915                 920                 925             


Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn 
    930                 935                 940                 


Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile 
945                 950                 955                 960 


Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser 
                965                 970                 975     


Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln 
            980                 985                 990         


Asp Thr Gln Glu Ile Lys Gln Arg  Val Val Phe Lys Tyr  Ser Gln Met 
        995                 1000                 1005             


Ile Asn  Ile Ser Asp Tyr Ile  Asn Arg Trp Ile Phe  Val Thr Ile 
    1010                 1015                 1020             


Thr Asn  Asn Arg Leu Asn Asn  Ser Lys Ile Tyr Ile  Asn Gly Arg 
    1025                 1030                 1035             


Leu Ile  Asp Gln Lys Pro Ile  Ser Asn Leu Gly Asn  Ile His Ala 
    1040                 1045                 1050             


Ser Asn  Asn Ile Met Phe Lys  Leu Asp Gly Cys Arg  Asp Thr His 
    1055                 1060                 1065             


Arg Tyr  Ile Trp Ile Lys Tyr  Phe Asn Leu Phe Asp  Lys Glu Leu 
    1070                 1075                 1080             


Asn Glu  Lys Glu Ile Lys Asp  Leu Tyr Asp Asn Gln  Ser Asn Ser 
    1085                 1090                 1095             


Gly Ile  Leu Lys Asp Phe Trp  Gly Asp Tyr Leu Gln  Tyr Asp Lys 
    1100                 1105                 1110             


Pro Tyr  Tyr Met Phe Asn Ala  Gly Asn Lys Asn Ser  Tyr Ile Lys 
    1115                 1120                 1125             


Leu Lys  Lys Asp Ser Pro Val  Gly Glu Ile Leu Gly  Pro Arg Gly 
    1130                 1135                 1140             


Ser Val  Met Thr Thr Asn Ile  Tyr Leu Asn Ser Ser  Leu Tyr Arg 
    1145                 1150                 1155             


Gly Glu  Lys Phe Ile Ile Arg  Arg Lys Ser Asn Ser  Gln Ser Ile 
    1160                 1165                 1170             


Asn Asp  Asp Ile Val Arg Asn  Glu Asp Tyr Ile Tyr  Leu Asp Phe 
    1175                 1180                 1185             


Phe Asn  Leu Asn Gln Glu Trp  Arg Val Tyr Thr Tyr  Lys Tyr Phe 
    1190                 1195                 1200             


Lys Lys  Glu Glu Glu Lys Leu  Phe Leu Ala Pro Ile  Ser Asp Ser 
    1205                 1210                 1215             


Asp Glu  Phe Tyr Asn Thr Ile  Gln Ile Lys Glu Tyr  Asp Glu Gln 
    1220                 1225                 1230             


Gly Thr  Asn Ser Cys Gln Leu  Leu Phe Lys Lys Asp  Glu Glu Ser 
    1235                 1240                 1245             


Thr Asp  Glu Ile Gly Leu Ile  Gly Ile His Arg Phe  Tyr Glu Ser 
    1250                 1255                 1260             


Gly Ile  Val Phe Glu Glu Tyr  Lys Asp Tyr Phe Cys  Ile Ser Lys 
    1265                 1270                 1275             


Trp Tyr  Leu Lys Glu Val Lys  Arg Lys Pro Tyr Asn  Leu Lys Leu 
    1280                 1285                 1290             


Gly Cys  Asn Trp Gln Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1295                 1300                 1305             


Arg Pro  Leu 
    1310     


<210>  6
<211>  437
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hc/TAB2.1, optimised loop 360

<400>  6

Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn 
1               5                   10                  15      


His Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser 
            20                  25                  30          


Lys Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn 
        35                  40                  45              


Leu Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr 
    50                  55                  60                  


Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro 
65                  70                  75                  80  


Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn 
                85                  90                  95      


Cys Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu 
            100                 105                 110         


Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val 
        115                 120                 125             


Phe Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp 
    130                 135                 140                 


Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr 
145                 150                 155                 160 


Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn 
                165                 170                 175     


Ile His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp 
            180                 185                 190         


Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu 
        195                 200                 205             


Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser 
    210                 215                 220                 


Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro 
225                 230                 235                 240 


Tyr Tyr Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys 
                245                 250                 255     


Lys Asp Ser Pro Val Gly Glu Ile Leu Gly Pro Arg Gly Ser Val Met 
            260                 265                 270         


Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Glu Lys Phe 
        275                 280                 285             


Ile Ile Arg Arg Lys Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val 
    290                 295                 300                 


Arg Asn Glu Asp Tyr Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu 
305                 310                 315                 320 


Trp Arg Val Tyr Thr Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu 
                325                 330                 335     


Phe Leu Ala Pro Ile Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln 
            340                 345                 350         


Ile Lys Glu Tyr Asp Glu Gln Pro Thr Tyr Ser Cys Gln Leu Leu Phe 
        355                 360                 365             


Lys Lys Asp Glu Glu Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His 
    370                 375                 380                 


Arg Phe Tyr Glu Ser Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe 
385                 390                 395                 400 


Cys Ile Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn 
                405                 410                 415     


Leu Lys Leu Gly Cys Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp 
            420                 425                 430         


Gly Glu Arg Pro Leu 
        435         


<210>  7
<211>  1314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hc/TAB2.1, codon optimised for expression in E. coli

<400>  7
aagaacatta tcaacaccag catcctgaac ctgcgctacg agagcaacca cctgatcgac       60

ctgagccgct acgcgagcaa gattaacatc ggtagcaagg tgaactttga cccgattgat      120

aaaaaccaga tccaactgtt caacctggaa agcagcaaga tcgaagtgat tctgaaaaac      180

gcgattgttt ataacagcat gtacgaaaac ttcagcacca gcttttggat ccgtattccg      240

aagtatttta acagcatcag cctgaacaac gaatacacca tcattaactg catggagaac      300

aacagcggtt ggaaagtgag cctgaactac ggcgaaatca tttggaccct gcaggacacc      360

caagagatca agcagcgtgt ggttttcaag tacagccaaa tgatcaacat cagcgattac      420

atcaaccgtt ggattttcgt taccatcacc aacaaccgtc tgaacaacag caagatctac      480

attaacggtc gtctgattga ccagaaaccg atcagcaacc tgggcaacat tcacgcgagc      540

aacaacatca tgttcaagct ggacggttgc cgtgataccc accgttatat ctggattaag      600

tacttcaacc tgtttgataa agagctgaac gaaaaggaga ttaaagacct gtatgataac      660

cagagcaaca gcggtatcct gaaggacttt tggggcgatt atctgcaata cgacaaaccg      720

tactatatgt tcaacgcggg taacaagaac agctacatta aactgaagaa agatagcccg      780

gtgggtgaaa tcctgggtcc gcgtggcagc gttatgacca ccaacatcta tctgaacagc      840

agcctgtacc gtggcgagaa gttcatcatt cgtcgtaaaa gcaacagcca gagcattaac      900

gacgatatcg tgcgtaacga agactacatt tatctggatt tctttaacct gaaccaagag      960

tggcgtgttt acacctacaa gtacttcaag aaagaggaag agaagctgtt cctggcgccg     1020

atcagcgaca gcgatgaatt ctacaacacc atccaaatca aggaatacga cgagcagccg     1080

acctatagct gccaactgct gttcaagaaa gacgaagaga gcaccgatga aatcggtctg     1140

atcggcattc accgtttcta cgagagcggc atcgtgttcg aagagtacaa ggattacttc     1200

tgcatcagca agtggtatct gaaagaggtt aagcgtaaac cgtacaacct gaaactgggc     1260

tgcaactggc aatttattcc ggtggatgat ggctggggtg aacgtccgct gtaa           1314


<210>  8
<211>  432
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hc/TAB2.1.1

<400>  8

Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu 
1               5                   10                  15      


Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp 
            20                  25                  30          


Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys 
        35                  40                  45              


Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu 
    50                  55                  60                  


Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser 
65                  70                  75                  80  


Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn 
                85                  90                  95      


Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu 
            100                 105                 110         


Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln 
        115                 120                 125             


Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile 
    130                 135                 140                 


Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu 
145                 150                 155                 160 


Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser Asn 
                165                 170                 175     


Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr Ile 
            180                 185                 190         


Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys Glu 
        195                 200                 205             


Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys Asp 
    210                 215                 220                 


Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Phe Asn 
225                 230                 235                 240 


Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys Lys Asp Ser Pro Val 
                245                 250                 255     


Gly Glu Ile Leu Gly Pro Arg Gly Ser Val Met Thr Thr Asn Ile Tyr 
            260                 265                 270         


Leu Asn Ser Ser Leu Tyr Arg Gly Glu Lys Phe Ile Ile Arg Arg Lys 
        275                 280                 285             


Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val Arg Asn Glu Asp Tyr 
    290                 295                 300                 


Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr Thr 
305                 310                 315                 320 


Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu Phe Leu Ala Pro Ile 
                325                 330                 335     


Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys Glu Tyr Asp 
            340                 345                 350         


Glu Gln Pro Thr Tyr Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu Glu 
        355                 360                 365             


Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Tyr Glu Ser 
    370                 375                 380                 


Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys Ile Ser Lys Trp 
385                 390                 395                 400 


Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu Lys Leu Gly Cys 
                405                 410                 415     


Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp Gly Glu Arg Pro Leu 
            420                 425                 430         


<210>  9
<211>  1299
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hc/TAB2.1.1, codon optimised for expression in E. coli

<400>  9
accagcatcc tgaacctgcg ctacgagagc aaccacctga tcgacctgag ccgctacgcg       60

agcaagatta acatcggtag caaggtgaac tttgacccga ttgataaaaa ccagatccaa      120

ctgttcaacc tggaaagcag caagatcgaa gtgattctga aaaacgcgat tgtttataac      180

agcatgtacg aaaacttcag caccagcttt tggatccgta ttccgaagta ttttaacagc      240

atcagcctga acaacgaata caccatcatt aactgcatgg agaacaacag cggttggaaa      300

gtgagcctga actacggcga aatcatttgg accctgcagg acacccaaga gatcaagcag      360

cgtgtggttt tcaagtacag ccaaatgatc aacatcagcg attacatcaa ccgttggatt      420

ttcgttacca tcaccaacaa ccgtctgaac aacagcaaga tctacattaa cggtcgtctg      480

attgaccaga aaccgatcag caacctgggc aacattcacg cgagcaacaa catcatgttc      540

aagctggacg gttgccgtga tacccaccgt tatatctgga ttaagtactt caacctgttt      600

gataaagagc tgaacgaaaa ggagattaaa gacctgtatg ataaccagag caacagcggt      660

atcctgaagg acttttgggg cgattatctg caatacgaca aaccgtacta tatgttcaac      720

gcgggtaaca agaacagcta cattaaactg aagaaagata gcccggtggg tgaaatcctg      780

ggtccgcgtg gcagcgttat gaccaccaac atctatctga acagcagcct gtaccgtggc      840

gagaagttca tcattcgtcg taaaagcaac agccagagca ttaacgacga tatcgtgcgt      900

aacgaagact acatttatct ggatttcttt aacctgaacc aagagtggcg tgtttacacc      960

tacaagtact tcaagaaaga ggaagagaag ctgttcctgg cgccgatcag cgacagcgat     1020

gaattctaca acaccatcca aatcaaggaa tacgacgagc agccgaccta tagctgccaa     1080

ctgctgttca agaaagacga agagagcacc gatgaaatcg gtctgatcgg cattcaccgt     1140

ttctacgaga gcggcatcgt gttcgaagag tacaaggatt acttctgcat cagcaagtgg     1200

tatctgaaag aggttaagcg taaaccgtac aacctgaaac tgggctgcaa ctggcaattt     1260

attccggtgg atgatggctg gggtgaacgt ccgctgtaa                            1299


<210>  10
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hc/TAB2.1.3, increased solubility

<400>  10

Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu 
1               5                   10                  15      


Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp 
            20                  25                  30          


Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys 
        35                  40                  45              


Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu 
    50                  55                  60                  


Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser 
65                  70                  75                  80  


Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn 
                85                  90                  95      


Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu 
            100                 105                 110         


Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln 
        115                 120                 125             


Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile 
    130                 135                 140                 


Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu 
145                 150                 155                 160 


Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser Asn 
                165                 170                 175     


Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr Ile 
            180                 185                 190         


Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys Glu 
        195                 200                 205             


Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys Asp 
    210                 215                 220                 


Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Phe Asn 
225                 230                 235                 240 


Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys Lys Asp Ser Pro Val 
                245                 250                 255     


Gly Glu Ile Leu Gly Pro Arg Gly Ser Val Met Thr Thr Asn Ile Tyr 
            260                 265                 270         


Leu Asn Ser Ser Leu Tyr Arg Gly Glu Lys Phe Ile Ile Arg Arg Lys 
        275                 280                 285             


Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val Arg Asn Glu Asp Tyr 
    290                 295                 300                 


Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr Thr 
305                 310                 315                 320 


Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu Phe Leu Ala Pro Ile 
                325                 330                 335     


Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys Glu Tyr Asp 
            340                 345                 350         


Glu Gln Pro Thr Tyr Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu Glu 
        355                 360                 365             


Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Asn Asn Lys 
    370                 375                 380                 


Asp Tyr Phe Cys Ile Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys 
385                 390                 395                 400 


Pro Tyr Asn Leu Lys Leu Gly Cys Asn Trp Gln Phe Ile Pro Val Asp 
                405                 410                 415     


Asp Gly Trp Gly Glu Arg Pro Leu 
            420                 


<210>  11
<211>  1275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hc/TAB2.1.3, codon optimised for expression in E. coli

<400>  11
accagcatcc tgaacctgcg ctacgagagc aaccacctga tcgacctgag ccgctacgcg       60

agcaagatta acatcggtag caaggtgaac tttgacccga ttgataaaaa ccagatccaa      120

ctgttcaacc tggaaagcag caagatcgaa gtgattctga aaaacgcgat tgtttataac      180

agcatgtacg aaaacttcag caccagcttt tggatccgta ttccgaagta ttttaacagc      240

atcagcctga acaacgaata caccatcatt aactgcatgg agaacaacag cggttggaaa      300

gtgagcctga actacggcga aatcatttgg accctgcagg acacccaaga gatcaagcag      360

cgtgtggttt tcaagtacag ccaaatgatc aacatcagcg attacatcaa ccgttggatt      420

ttcgttacca tcaccaacaa ccgtctgaac aacagcaaga tctacattaa cggtcgtctg      480

attgaccaga aaccgatcag caacctgggc aacattcacg cgagcaacaa catcatgttc      540

aagctggacg gttgccgtga tacccaccgt tatatctgga ttaagtactt caacctgttt      600

gataaagagc tgaacgaaaa ggagattaaa gacctgtatg ataaccagag caacagcggt      660

atcctgaagg acttttgggg cgattatctg caatacgaca aaccgtacta tatgttcaac      720

gcgggtaaca agaacagcta cattaaactg aagaaagata gcccggtggg tgaaatcctg      780

ggtccgcgtg gcagcgttat gaccaccaac atctatctga acagcagcct gtaccgtggc      840

gagaagttca tcattcgtcg taaaagcaac agccagagca ttaacgacga tatcgtgcgt      900

aacgaagact acatttatct ggatttcttt aacctgaacc aagagtggcg tgtttacacc      960

tacaagtact tcaagaaaga ggaagagaag ctgttcctgg cgccgatcag cgacagcgat     1020

gaattctaca acaccatcca aatcaaggaa tacgacgagc agccgaccta tagctgccaa     1080

ctgctgttca agaaagacga agagagcacc gatgaaatcg gtctgatcgg cattcaccgt     1140

ttcaacaaca aggattactt ctgcatcagc aagtggtatc tgaaagaggt taagcgtaaa     1200

ccgtacaacc tgaaactggg ctgcaactgg caatttattc cggtggatga tggctggggt     1260

gaacgtccgc tgtaa                                                      1275


<210>  12
<211>  1343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BoNT/TAB2.1.3, full-length active protein

<400>  12

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Ala 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Ala Lys Thr Phe Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Ala Gly Ala Gly Lys Ser Leu Val Pro Arg 
        435                 440                 445             


Gly Ser Ala Gly Ala Gly Ala Leu Asn Asp Leu Cys Ile Lys Val Asn 
    450                 455                 460                 


Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp 
465                 470                 475                 480 


Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala 
                485                 490                 495     


Glu Glu Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe 
            500                 505                 510         


Asn Phe Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser 
        515                 520                 525             


Asp Ile Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro 
    530                 535                 540                 


Asn Gly Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu 
545                 550                 555                 560 


Arg Ala Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn 
                565                 570                 575     


Ser Val Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe 
            580                 585                 590         


Ser Ser Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met 
        595                 600                 605             


Phe Leu Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr 
    610                 615                 620                 


Ser Glu Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile 
625                 630                 635                 640 


Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp 
                645                 650                 655     


Asp Phe Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu 
            660                 665                 670         


Phe Ile Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val 
        675                 680                 685             


Ser Tyr Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala 
    690                 695                 700                 


Leu Ser Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val 
705                 710                 715                 720 


Thr Asn Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys 
                725                 730                 735     


Lys Met Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile 
            740                 745                 750         


Ile Asn Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile 
        755                 760                 765             


Asn Phe Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn 
    770                 775                 780                 


Lys Ala Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser 
785                 790                 795                 800 


Tyr Leu Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp 
                805                 810                 815     


Phe Asp Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn 
            820                 825                 830         


Arg Gly Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn 
        835                 840                 845             


Asn Thr Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp 
    850                 855                 860                 


Asn Gln Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile 
865                 870                 875                 880 


Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp 
                885                 890                 895     


Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe 
            900                 905                 910         


Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser 
        915                 920                 925             


Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr 
    930                 935                 940                 


Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn 
945                 950                 955                 960 


Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn 
                965                 970                 975     


Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr 
            980                 985                 990         


Leu Gln Asp Thr Gln Glu Ile Lys  Gln Arg Val Val Phe  Lys Tyr Ser 
        995                 1000                 1005             


Gln Met  Ile Asn Ile Ser Asp  Tyr Ile Asn Arg Trp  Ile Phe Val 
    1010                 1015                 1020             


Thr Ile  Thr Asn Asn Arg Leu  Asn Asn Ser Lys Ile  Tyr Ile Asn 
    1025                 1030                 1035             


Gly Arg  Leu Ile Asp Gln Lys  Pro Ile Ser Asn Leu  Gly Asn Ile 
    1040                 1045                 1050             


His Ala  Ser Asn Asn Ile Met  Phe Lys Leu Asp Gly  Cys Arg Asp 
    1055                 1060                 1065             


Thr His  Arg Tyr Ile Trp Ile  Lys Tyr Phe Asn Leu  Phe Asp Lys 
    1070                 1075                 1080             


Glu Leu  Asn Glu Lys Glu Ile  Lys Asp Leu Tyr Asp  Asn Gln Ser 
    1085                 1090                 1095             


Asn Ser  Gly Ile Leu Lys Asp  Phe Trp Gly Asp Tyr  Leu Gln Tyr 
    1100                 1105                 1110             


Asp Lys  Pro Tyr Tyr Met Phe  Asn Ala Gly Asn Lys  Asn Ser Tyr 
    1115                 1120                 1125             


Ile Lys  Leu Lys Lys Asp Ser  Pro Val Gly Glu Ile  Leu Gly Pro 
    1130                 1135                 1140             


Arg Gly  Ser Val Met Thr Thr  Asn Ile Tyr Leu Asn  Ser Ser Leu 
    1145                 1150                 1155             


Tyr Arg  Gly Glu Lys Phe Ile  Ile Arg Arg Lys Ser  Asn Ser Gln 
    1160                 1165                 1170             


Ser Ile  Asn Asp Asp Ile Val  Arg Asn Glu Asp Tyr  Ile Tyr Leu 
    1175                 1180                 1185             


Asp Phe  Phe Asn Leu Asn Gln  Glu Trp Arg Val Tyr  Thr Tyr Lys 
    1190                 1195                 1200             


Tyr Phe  Lys Lys Glu Glu Glu  Lys Leu Phe Leu Ala  Pro Ile Ser 
    1205                 1210                 1215             


Asp Ser  Asp Glu Phe Tyr Asn  Thr Ile Gln Ile Lys  Glu Tyr Asp 
    1220                 1225                 1230             


Glu Gln  Gly Thr Asn Ser Cys  Gln Leu Leu Phe Lys  Lys Asp Glu 
    1235                 1240                 1245             


Glu Ser  Thr Asp Glu Ile Gly  Leu Ile Gly Ile His  Arg Phe Tyr 
    1250                 1255                 1260             


Glu Ser  Gly Ile Val Phe Glu  Glu Tyr Lys Asp Tyr  Phe Cys Ile 
    1265                 1270                 1275             


Ser Lys  Trp Tyr Leu Lys Glu  Val Lys Arg Lys Pro  Tyr Asn Leu 
    1280                 1285                 1290             


Lys Leu  Gly Cys Asn Trp Gln  Phe Ile Pro Val Asp  Asp Gly Trp 
    1295                 1300                 1305             


Gly Glu  Arg Pro Leu Val Pro  Arg Gly Ser Ala Asn  Ser Ser Ser 
    1310                 1315                 1320             


Val Asp  Lys Leu Trp Ser His  Pro Gln Phe Glu Lys  Leu Glu His 
    1325                 1330                 1335             


His His  His His His 
    1340             


<210>  13
<211>  4032
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BoNT/TAB2.1.3, sequence included in pEt29(a) vector for 
       expression in E. coli

<400>  13
atgccatttg tgaacaagca gtttaactat aaggacccgg tgaacggtgt ggatatcgcg       60

tatatcaaaa tcccgaatgc gggccagatg caaccagtca aggcgttcaa gattcataac      120

aagatttggg ttattccgga acgtgatacc ttcaccaatc cggaagaagg cgacttaaac      180

ccgccgccag aagccaaaca agtgccggtg agctactatg atagcacgta tcttagcacc      240

gataatgaaa aagacaatta cctgaagggc gtgaccaagt tgttcgagcg catctacagt      300

accgacttag gccgcatgtt gttgacgagc atcgttcgcg gtatcccgtt ctggggcggc      360

tcgaccattg ataccgagtt gaaagtcatt gacacgaact gtatcaatgt tatccaaccg      420

gacggcagtt atcgcagcga ggagttaaat ttggtcatca tcggtccaag cgcagatatt      480

attcagttcg aatgcaagag cttcggccat gaggtcttga atttgacgcg caacggttac      540

ggcagcaccc aatacatccg ctttagcccg gatttcacct ttggcttcga ggagagcttg      600

gaggtggaca ccaacccgct gttaggtgcc ggcaaattcg caaccgaccc ggcagtgacg      660

ttggcgcacg cgttgattca tgcgggtcac cgcttatacg gtatcgcgat caatccgaat      720

cgcgtcttta aagtcaatac caacgcgtac tacgaaatga gcggcttaga ggttagcttt      780

gaagaattac gcaccttcgg tggccacgac gccaagttca tcgacagcct gcaggaaaat      840

gagttccgct tgtactatta caataaattc aaggacatcg cgagcacctt aaataaagca      900

aagagcattg tgggcaccac cgcaagcttg cagtacatga agaacgtatt taaggaaaaa      960

tatttgttgt cggaggatac cagcgggaaa ttcagcgtcg ataagctgaa attcgacaaa     1020

ttgtataaaa tgctgaccga gatttacacc gaggataact tcgtcaagtt ttttaaggtg     1080

ttaaatgcga agaccttttt aaactttgat aaagcggtgt ttaaaattaa tatcgtgccg     1140

aaggtgaatt acaccatcta cgatggtttc aatttacgca acacgaatct ggcggcgaat     1200

tttaatggcc aaaacaccga aattaacaac atgaacttta cgaagttaaa gaatttcacg     1260

ggcttattcg aattctacaa gttattatgc gtgcgcggca tcattaccag caaggcaggt     1320

gcgggcaagt ccttggttcc gcgtggcagc gccggcgccg gcgcgctcaa tgatctgtgt     1380

attaaagtca ataactggga cctgttcttc agcccgagcg aggataactt taccaacgac     1440

ttaaacaaag gcgaggagat cacgagcgat acgaacatcg aggcggcgga ggaaaatatt     1500

agcctggacc tcattcagca gtactatctg acgttcaatt ttgacaatga gccggagaac     1560

atcagcattg aaaatctcag cagcgacatc atcggtcagt tggaactgat gccgaacatt     1620

gaacgctttc cgaacggcaa aaaatatgaa ctggacaagt ataccatgtt ccattactta     1680

cgcgcacagg aatttgagca cggcaagagc cgcattgcgc tgaccaatag cgttaacgag     1740

gccttgttaa atccgagccg tgtctacacg ttcttcagca gcgattatgt caaaaaagtg     1800

aacaaggcga ccgaagccgc gatgtttttg ggctgggtcg agcaattggt ttacgatttt     1860

accgacgaaa ccagcgaggt gagcacgacc gacaaaattg cagatatcac catcatcatt     1920

ccgtacatcg gtccggcgct caatatcggc aatatgttat acaaggacga ctttgtgggc     1980

gcgctgatct ttagcggcgc ggttatctta ttagaattca tcccggagat cgcaatcccg     2040

gtcttgggca cctttgcgtt ggtgagctat atcgcgaata aagtgctcac ggtccaaacc     2100

atcgataacg cgctcagcaa gcgtaatgag aaatgggacg aggtttataa gtatatcgtg     2160

accaactggt tagcaaaagt caatacgcag atcgatctca tccgcaaaaa aatgaaagaa     2220

gccttggaaa atcaagcgga ggcaaccaaa gccatcatta attaccagta taaccaatat     2280

accgaagaag aaaaaaacaa tatcaacttc aatatcgatg atttgagcag caaactgaac     2340

gagagcatta acaaagcgat gattaacatc aacaagttct tgaatcaatg cagcgtgagc     2400

tatctcatga acagcatgat cccgtatggc gtcaaacgct tggaagattt tgacgccagc     2460

ctgaaagatg cgctcctcaa gtatatttat gacaaccgcg gcaccctcat tggccaggtg     2520

gaccgcttga aggataaagt gaacaatacg ctcagcacgg atatcccgtt ccagctgagc     2580

aagtacgtcg acaaccagcg cttactgagc acctttaccg agtatatcaa gaacatcatt     2640

aataccagca tcctcaactt gcgctatgag agcaatcacc tgatcgacct cagccgctac     2700

gccagcaaga tcaacatcgg cagcaaggtc aatttcgacc cgatcgataa gaatcagatc     2760

caattgttta acctggaaag cagcaagatc gaggttatct tgaagaacgc gattgtgtac     2820

aacagcatgt atgagaactt tagcaccagc ttctggattc gtatcccgaa atatttcaac     2880

agcattagcc tgaacaacga gtacaccatt atcaactgca tggaaaacaa cagcggttgg     2940

aaggtgagcc tgaactacgg cgagattatc tggaccctgc aggacaccca agaaatcaag     3000

cagcgtgtgg ttttcaagta cagccaaatg atcaacatca gcgattacat taaccgttgg     3060

atctttgtta ccattaccaa caaccgtctg aacaacagca aaatttacat caacggtcgt     3120

ctgatcgacc agaagccgat tagcaacctg ggcaacatcc acgcgagcaa caacattatg     3180

ttcaagctgg acggttgccg tgatacccac cgttatattt ggatcaagta cttcaacctg     3240

ttcgataagg agctgaacga gaaggaaatc aaagacctgt atgataacca gagcaacagc     3300

ggtattctga aagacttctg gggcgattac ctgcaatatg acaagccgta ttacatgttt     3360

aacgcgggta acaagaacag ctacatcaaa ctgaagaaag atagcccggt gggtgaaatt     3420

ctgggtccgc gtggcagcgt tatgaccacc aacatctatc tgaacagcag cctgtaccgt     3480

ggcgaaaagt tcattatccg tcgtaaaagc aacagccaga gcatcaacga cgatattgtg     3540

cgtaacgagg actatatcta cctggatttc tttaacctga accaagaatg gcgtgtttac     3600

acctacaagt acttcaagaa agaagaggaa aagctgtttc tggcgccgat tagcgacagc     3660

gatgaattct ataacaccat tcagatcaaa gagtacgacg aacagggtac caacagctgc     3720

caactgctgt ttaagaaaga cgaggaaagc accgatgaga tcggtctgat tggcatccac     3780

cgtttttacg aaagcggcat cgtgttcgag gaatacaagg attacttctg catcagcaag     3840

tggtatctga aagaggttaa gcgtaaaccg tacaacctga aactgggctg caactggcaa     3900

tttattccgg tggatgatgg ctggggtgaa cgtccactag tgccacgcgg ttccgcgaat     3960

tcgagctccg tcgacaagct ttggagccac ccgcagttcg aaaaactcga gcaccaccac     4020

caccaccact ga                                                         4032


<210>  14
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hSytI peptide

<400>  14

Gly Glu Gly Lys Glu Asp Ala Phe Ser Lys Leu Lys Glu Lys Phe Met 
1               5                   10                  15      


Asn Glu Leu His Lys 
            20      


