                         SEQUENCE LISTING

<110>  Glaxo Smith Kline Biologicals SA
 
<120>  IMMUNOGENIC COMPOSITION

<130>  VB66439

<150>  1802339.0
<151>  2018-02-13

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  522
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Ala Ser Glu Asn Ser Val Thr Gln Ser Asp Ser Ala Ser Asn Glu Ser 
1               5                   10                  15      


Lys Ser Asn Asp Ser Ser Ser Val Ser Ala Ala Pro Lys Thr Asp Asp 
            20                  25                  30          


Thr Asn Val Ser Asp Thr Lys Thr Ser Ser Asn Thr Asn Asn Gly Glu 
        35                  40                  45              


Thr Ser Val Ala Gln Asn Pro Ala Gln Gln Glu Thr Thr Gln Ser Ser 
    50                  55                  60                  


Ser Thr Asn Ala Thr Thr Glu Glu Thr Pro Val Thr Gly Glu Ala Thr 
65                  70                  75                  80  


Thr Thr Thr Thr Asn Gln Ala Asn Thr Pro Ala Thr Thr Gln Ser Ser 
                85                  90                  95      


Asn Thr Asn Ala Glu Glu Leu Val Asn Gln Thr Ser Asn Glu Thr Thr 
            100                 105                 110         


Phe Asn Asp Thr Asn Thr Val Ser Ser Val Asn Ser Pro Gln Asn Ser 
        115                 120                 125             


Thr Asn Ala Glu Asn Val Ser Thr Thr Gln Asp Thr Ser Thr Glu Ala 
    130                 135                 140                 


Thr Pro Ser Asn Asn Glu Ser Ala Pro Gln Ser Thr Asp Ala Ser Asn 
145                 150                 155                 160 


Lys Asp Val Val Asn Gln Ala Val Asn Thr Ser Ala Pro Arg Met Arg 
                165                 170                 175     


Ala Phe Ser Leu Ala Ala Val Ala Ala Asp Ala Pro Ala Ala Gly Thr 
            180                 185                 190         


Asp Ile Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly 
        195                 200                 205             


Thr Thr Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly 
    210                 215                 220                 


Phe Ser Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr 
225                 230                 235                 240 


Val Pro Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val 
                245                 250                 255     


Pro Pro Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp 
            260                 265                 270         


Ser Asp Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys 
        275                 280                 285             


Asp Asp Val Lys Ala Thr Leu Thr Met Pro Ala Tyr Ile Asp Pro Glu 
    290                 295                 300                 


Asn Val Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser 
305                 310                 315                 320 


Thr Thr Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys 
                325                 330                 335     


Phe Tyr Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr 
            340                 345                 350         


Asn Asn Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn 
        355                 360                 365             


Val Ile Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser 
    370                 375                 380                 


Asn Ala Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val 
385                 390                 395                 400 


Asp Asn Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn 
                405                 410                 415     


Phe Glu Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn 
            420                 425                 430         


Gln Tyr Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro 
        435                 440                 445             


Tyr Ile Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp 
    450                 455                 460                 


Leu Ala Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp 
465                 470                 475                 480 


Arg Ser Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly 
                485                 490                 495     


Ser Gly Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu 
            500                 505                 510         


Pro Gly Glu Ile Glu Pro Ile Pro Glu Asp 
        515                 520         


<210>  2
<211>  340
<212>  PRT
<213>  Staphylococcus aureus

<400>  2

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Pro Ala Tyr Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Ile Pro Glu Asp 
            340 


<210>  3
<211>  293
<212>  PRT
<213>  Staphylococcus aureus

<400>  3

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  4
<211>  516
<212>  PRT
<213>  Staphylococcus aureus

<400>  4

Met Lys Lys Lys Asn Ile Tyr Ser Ile Arg Lys Leu Gly Val Gly Ile 
1               5                   10                  15      


Ala Ser Val Thr Leu Gly Thr Leu Leu Ile Ser Gly Gly Val Thr Pro 
            20                  25                  30          


Ala Ala Asn Ala Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr 
        35                  40                  45              


Gln Val Leu Asn Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe 
    50                  55                  60                  


Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly 
65                  70                  75                  80  


Glu Ala Gln Lys Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln 
                85                  90                  95      


Gln Asn Asn Phe Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu 
            100                 105                 110         


Asn Met Pro Asn Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser 
        115                 120                 125             


Leu Lys Asp Asp Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys 
    130                 135                 140                 


Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys 
145                 150                 155                 160 


Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn 
                165                 170                 175     


Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser 
            180                 185                 190         


Gln Ser Ala Asn Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln 
        195                 200                 205             


Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe 
    210                 215                 220                 


Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly 
225                 230                 235                 240 


Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu 
                245                 250                 255     


Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn 
            260                 265                 270         


Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu 
        275                 280                 285             


Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys 
    290                 295                 300                 


Asp Asp Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu 
305                 310                 315                 320 


Asn Asp Ala Gln Ala Pro Lys Glu Glu Asp Asn Asn Lys Pro Gly Lys 
                325                 330                 335     


Glu Asp Asn Asn Lys Pro Gly Lys Glu Asp Asn Asn Lys Pro Gly Lys 
            340                 345                 350         


Glu Asp Asn Asn Lys Pro Gly Lys Glu Asp Asn Asn Lys Pro Gly Lys 
        355                 360                 365             


Glu Asp Gly Asn Lys Pro Gly Lys Glu Asp Asn Lys Lys Pro Gly Lys 
    370                 375                 380                 


Glu Asp Gly Asn Lys Pro Gly Lys Glu Asp Asn Lys Lys Pro Gly Lys 
385                 390                 395                 400 


Glu Asp Gly Asn Lys Pro Gly Lys Glu Asp Gly Asn Lys Pro Gly Lys 
                405                 410                 415     


Glu Asp Gly Asn Gly Val His Val Val Lys Pro Gly Asp Thr Val Asn 
            420                 425                 430         


Asp Ile Ala Lys Ala Asn Gly Thr Thr Ala Asp Lys Ile Ala Ala Asp 
        435                 440                 445             


Asn Lys Leu Ala Asp Lys Asn Met Ile Lys Pro Gly Gln Glu Leu Val 
    450                 455                 460                 


Val Asp Lys Lys Gln Pro Ala Asn His Ala Asp Ala Asn Lys Ala Gln 
465                 470                 475                 480 


Ala Leu Pro Glu Thr Gly Glu Glu Asn Pro Phe Ile Gly Thr Thr Val 
                485                 490                 495     


Phe Gly Gly Leu Ser Leu Ala Leu Gly Ala Ala Leu Leu Ala Gly Arg 
            500                 505                 510         


Arg Arg Glu Leu 
        515     


<210>  5
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfA N2N3 P116S/Y118A

<400>  5

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Ile Pro Glu Asp 
            340 


<210>  6
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfA N2N3 P116S/Y118A  with PglB

<400>  6

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
            340                 345     


<210>  7
<211>  347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfA N2N3 P116S/Y118A

<400>  7

Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln 
1               5                   10                  15      


Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro 
            20                  25                  30          


His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn 
        35                  40                  45              


Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu 
    50                  55                  60                  


Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala 
65                  70                  75                  80  


Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val 
                85                  90                  95      


Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala 
            100                 105                 110         


Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr 
        115                 120                 125             


Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys 
    130                 135                 140                 


Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser 
145                 150                 155                 160 


Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg 
                165                 170                 175     


Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val 
            180                 185                 190         


Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp 
        195                 200                 205             


Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp 
    210                 215                 220                 


Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr 
225                 230                 235                 240 


Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu 
                245                 250                 255     


Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val 
            260                 265                 270         


Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser 
        275                 280                 285             


Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp 
    290                 295                 300                 


Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile 
305                 310                 315                 320 


Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu 
                325                 330                 335     


Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
            340                 345         


<210>  8
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature Hla H35L

<400>  8

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  9
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature Hla H35L with PglB consensus sequencesubstituted for 
       residue K131

<400>  9

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly Leu Ile Gly Ala 
    130                 135                 140                 


Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys 
145                 150                 155                 160 


Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile 
                165                 170                 175     


Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser 
            180                 185                 190         


Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly 
        195                 200                 205             


Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser 
    210                 215                 220                 


Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met 
225                 230                 235                 240 


Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu 
                245                 250                 255     


Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys 
            260                 265                 270         


Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr 
        275                 280                 285             


Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 


<210>  10
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature Hla H35L/H48C/G122C

<400>  10

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  11
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature Hla H35L/H48C/G122C with PglB consensus sequence 
       substituted for residue K131

<400>  11

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly Leu Ile Gly Ala 
    130                 135                 140                 


Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys 
145                 150                 155                 160 


Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile 
                165                 170                 175     


Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser 
            180                 185                 190         


Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly 
        195                 200                 205             


Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser 
    210                 215                 220                 


Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met 
225                 230                 235                 240 


Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu 
                245                 250                 255     


Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys 
            260                 265                 270         


Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr 
        275                 280                 285             


Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 


<210>  12
<211>  306
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hla H35L/H48C/G122C with N-terminal S, C-terminal GSHRHR, and 
       KDQNRTK substituted for residue K131

<400>  12

Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly 
1               5                   10                  15      


Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu 
            20                  25                  30          


Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn 
        35                  40                  45              


Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly 
    50                  55                  60                  


Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala 
65                  70                  75                  80  


Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val 
                85                  90                  95      


Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu 
            100                 105                 110         


Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp 
        115                 120                 125             


Asp Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly Leu Ile Gly 
    130                 135                 140                 


Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe 
145                 150                 155                 160 


Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val 
                165                 170                 175     


Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp 
            180                 185                 190         


Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn 
        195                 200                 205             


Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser 
    210                 215                 220                 


Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr 
225                 230                 235                 240 


Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr 
                245                 250                 255     


Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp 
            260                 265                 270         


Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg 
        275                 280                 285             


Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn Gly Ser His Arg 
    290                 295                 300                 


His Arg 
305     


<210>  13
<211>  291
<212>  PRT
<213>  Staphylococcus aureus

<400>  13

Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu Asn 
1               5                   10                  15      


Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys 
        35                  40                  45              


Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln Gln Asn Asn Phe 
    50                  55                  60                  


Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn 
65                  70                  75                  80  


Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp 
                85                  90                  95      


Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu 
            100                 105                 110         


Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn 
        115                 120                 125             


Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg 
    130                 135                 140                 


Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn 
145                 150                 155                 160 


Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala 
                165                 170                 175     


Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu 
            180                 185                 190         


His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser 
        195                 200                 205             


Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys 
    210                 215                 220                 


Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys 
225                 230                 235                 240 


Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr 
                245                 250                 255     


Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser 
            260                 265                 270         


Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        275                 280                 285             


Ala Pro Lys 
    290     


<210>  14
<211>  56
<212>  PRT
<213>  Staphylococcus aureus

<400>  14

Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu Asn 
1               5                   10                  15      


Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys 
        35                  40                  45              


Leu Asn Asp Ser Gln Ala Pro Lys 
    50                  55      


<210>  15
<211>  61
<212>  PRT
<213>  Staphylococcus aureus

<400>  15

Ala Asp Ala Gln Gln Asn Asn Phe Asn Lys Asp Gln Gln Ser Ala Phe 
1               5                   10                  15      


Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Ala Gln Arg Asn Gly 
            20                  25                  30          


Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Thr Asn Val Leu 
        35                  40                  45              


Gly Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55                  60      


<210>  16
<211>  58
<212>  PRT
<213>  Staphylococcus aureus

<400>  16

Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ser Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55              


<210>  17
<211>  58
<212>  PRT
<213>  Staphylococcus aureus

<400>  17

Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ser Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55              


<210>  18
<211>  58
<212>  PRT
<213>  Staphylococcus aureus

<400>  18

Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  19
<211>  56
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA E domain with QQ-KK substitutions at positions 7 and 8 and 
       DD-AA substitutions at positions 34 and 35

<400>  19

Ala Gln His Asp Glu Ala Lys Lys Asn Ala Phe Tyr Gln Val Leu Asn 
1               5                   10                  15      


Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Ala Ala Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys 
        35                  40                  45              


Leu Asn Asp Ser Gln Ala Pro Lys 
    50                  55      


<210>  20
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA D domain with QQ-KK substitutionsat positions 12 and 13 and 
       DD-AA substitutions atpositions 39and 40

<400>  20

Ala Asp Ala Gln Gln Asn Asn Phe Asn Lys Asp Lys Lys Ser Ala Phe 
1               5                   10                  15      


Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Ala Gln Arg Asn Gly 
            20                  25                  30          


Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser Gln Ser Thr Asn Val Leu 
        35                  40                  45              


Gly Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55                  60      


<210>  21
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA A domain with QQ-KK substitutionsat positions 9 and108 and 
       DD-AA substitutions atpositions 36and 37

<400>  21

Ala Asp Asn Asn Phe Asn Lys Glu Lys Lys Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn Leu Leu Ser Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55              


<210>  22
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA B domain with QQ-KK substitutionsat positions 9 and 10 and 
       DD-AA substitutions atpositions 36and 37

<400>  22

Ala Asp Asn Lys Phe Asn Lys Glu Lys Lys Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  23
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA C domain with QQ-KK substitutionsat positions 9 and 10 and 
       DD-AA substitutions atpositions 36and 37

<400>  23

Ala Asp Asn Lys Phe Asn Lys Glu Lys Lys Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Ala Ala Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
    50                  55              


<210>  24
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA D domain with QQ-KR substitutions at positions 4 and 5, QQ-KK
       substitutionsat positions 12 and 13 and DD-AA substitutions 
       atpositions 39and 40

<400>  24

Ala Asp Ala Lys Arg Asn Asn Phe Asn Lys Asp Lys Lys Ser Ala Phe 
1               5                   10                  15      


Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Ala Gln Arg Asn Gly 
            20                  25                  30          


Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser Gln Ser Thr Asn Val Leu 
        35                  40                  45              


Gly Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55                  60      


<210>  25
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA D domain with QQ-KR substitutions at positions  4 and 5

<400>  25

Ala Asp Ala Lys Arg Asn Asn Phe Asn Lys Asp Gln Gln Ser Ala Phe 
1               5                   10                  15      


Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Ala Gln Arg Asn Gly 
            20                  25                  30          


Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Thr Asn Val Leu 
        35                  40                  45              


Gly Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys 
    50                  55                  60      


<210>  26
<211>  291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA IgG binding portion with the substitutions of SEQ ID NOs 
       19-23

<400>  26

Ala Gln His Asp Glu Ala Lys Lys Asn Ala Phe Tyr Gln Val Leu Asn 
1               5                   10                  15      


Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Ala Ala Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys 
        35                  40                  45              


Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln Gln Asn Asn Phe 
    50                  55                  60                  


Asn Lys Asp Lys Lys Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn 
65                  70                  75                  80  


Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala 
                85                  90                  95      


Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu 
            100                 105                 110         


Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Lys Lys Asn 
        115                 120                 125             


Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg 
    130                 135                 140                 


Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn 
145                 150                 155                 160 


Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala 
                165                 170                 175     


Asp Asn Lys Phe Asn Lys Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu 
            180                 185                 190         


His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser 
        195                 200                 205             


Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys 
    210                 215                 220                 


Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys 
225                 230                 235                 240 


Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr 
                245                 250                 255     


Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser 
            260                 265                 270         


Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        275                 280                 285             


Ala Pro Lys 
    290     


<210>  27
<211>  291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpA IgG binding portion with the substitutions of SEQ ID NOs 19 
       and 21 24

<400>  27

Ala Gln His Asp Glu Ala Lys Lys Asn Ala Phe Tyr Gln Val Leu Asn 
1               5                   10                  15      


Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Ala Ala Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys 
        35                  40                  45              


Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Lys Arg Asn Asn Phe 
    50                  55                  60                  


Asn Lys Asp Lys Lys Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn 
65                  70                  75                  80  


Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala 
                85                  90                  95      


Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu 
            100                 105                 110         


Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Lys Lys Asn 
        115                 120                 125             


Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg 
    130                 135                 140                 


Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn 
145                 150                 155                 160 


Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala 
                165                 170                 175     


Asp Asn Lys Phe Asn Lys Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu 
            180                 185                 190         


His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser 
        195                 200                 205             


Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys 
    210                 215                 220                 


Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys 
225                 230                 235                 240 


Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr 
                245                 250                 255     


Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser 
            260                 265                 270         


Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        275                 280                 285             


Ala Pro Lys 
    290     


<210>  28
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PglB consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa = Ser or Thr

<400>  28

Xaa Xaa Asn Xaa Xaa 
1               5   


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PglB consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa = Ser or Thr

<400>  29

Lys Xaa Xaa Asn Xaa Xaa Lys 
1               5           


<210>  30
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PglB consensus sequence

<400>  30

Lys Asp Gln Asn Arg Thr Lys 
1               5           


<210>  31
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PglB consensus sequence

<400>  31

Lys Asp Gln Asn Ala Thr Lys 
1               5           


<210>  32
<211>  349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A at I337, N-terminal S, C-terminal  GS

<400>  32

Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln 
1               5                   10                  15      


Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro 
            20                  25                  30          


His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn 
        35                  40                  45              


Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu 
    50                  55                  60                  


Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala 
65                  70                  75                  80  


Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val 
                85                  90                  95      


Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala 
            100                 105                 110         


Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr 
        115                 120                 125             


Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys 
    130                 135                 140                 


Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser 
145                 150                 155                 160 


Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg 
                165                 170                 175     


Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val 
            180                 185                 190         


Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp 
        195                 200                 205             


Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp 
    210                 215                 220                 


Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr 
225                 230                 235                 240 


Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu 
                245                 250                 255     


Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val 
            260                 265                 270         


Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser 
        275                 280                 285             


Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp 
    290                 295                 300                 


Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile 
305                 310                 315                 320 


Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu 
                325                 330                 335     


Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp Gly Ser 
            340                 345                 


