                         SEQUENCE LISTING

<110>  Universittsmedizin der Johannes Gutenberg-Universitt 
       Mainz
 
<120>  Vaccines for use in the prevention or treatment of leishmaniasis

<130>  Leishmaniasis

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Leishmania major

<400>  1

Cys Ala Gln Ala Asn Val Ser Leu Ile 
1               5                   


<210>  2
<211>  807
<212>  PRT
<213>  Leishmania major

<400>  2

Met Ser Glu Ile Ser Phe Ser Glu Met Ser Leu His Glu Val Pro Leu 
1               5                   10                  15      


Ala Ser Glu Ala Thr Pro Ser Ser Ala Val Val Leu Gly Gly Arg Thr 
            20                  25                  30          


Asp Leu Arg Val Gly Asp Phe Val Gly Phe Gln Arg Ile Ser Pro Ser 
        35                  40                  45              


Lys Glu Trp Lys Leu Ser Leu Gly Lys Val Ile Ala Leu Pro Thr Ala 
    50                  55                  60                  


Leu Thr Val Lys Val Ala His Phe Asp Ala Val Asn Glu Thr Thr Val 
65                  70                  75                  80  


Thr Gln Asp Pro Ile Ser Glu Gln Met Val Arg Gln Lys Ala Ala Glu 
                85                  90                  95      


Thr Gln Lys Met Asp Ile Trp His Asn Arg Glu Ala Leu Arg Glu Asn 
            100                 105                 110         


Leu Ala Gln Glu Ser Ala Lys Glu Lys Ala Ala Thr Gln Arg Leu Leu 
        115                 120                 125             


Ala Ala Arg Asp Gln Thr Val Thr Arg Gln Met Ala Thr Gly Asp Lys 
    130                 135                 140                 


Val Thr Ala Ser Leu Glu Asn Leu Asp Asn Ala Arg Arg Arg Leu Arg 
145                 150                 155                 160 


Glu Val Pro Val Lys Glu Trp Gly Ala Leu Arg Ser Ser Tyr Leu Pro 
                165                 170                 175     


Ser Ala Glu Ile Leu Gly Val Met Arg Ser Val Met Leu Ile Leu Tyr 
            180                 185                 190         


Glu Asp Ser Val Thr Lys Trp Glu His Ile Gln Glu Val Met His Arg 
        195                 200                 205             


Gln Asp Phe Met Glu Arg Ile Leu Gly Trp Asp Cys Thr Ala Thr Pro 
    210                 215                 220                 


Met Ser Leu Ser Arg Arg Lys Arg Ile Val Ala Leu Cys Ala Gly Lys 
225                 230                 235                 240 


Asp Val Glu Gly Ala Pro Ser Lys Lys Leu His Pro Ser Gly Thr Arg 
                245                 250                 255     


Ser Arg Ser Pro Ser Arg Pro Leu Val Val Ala Ala Lys Ser Thr Asn 
            260                 265                 270         


Pro Ser Asn Val Val Ile Leu Asp Lys Asn Val Arg Thr Trp Ile Asn 
        275                 280                 285             


Ala Gln Leu Ser Cys Ser Glu Ala Ala Arg Glu Gln Glu Met Met Ile 
    290                 295                 300                 


Asn Ser Cys Phe Ala Glu Gln Gln Glu Gln Arg Ala Leu Leu Arg Glu 
305                 310                 315                 320 


Ile Asn Asp Met Arg Val Gly Ile Ser Thr Ile Glu Val Gln Met Leu 
                325                 330                 335     


Glu Met Lys Asn Ala Ile Leu Gly Ile Asp Asp Ala Pro Arg Pro Ile 
            340                 345                 350         


Met Pro Leu Asp Ala Tyr Pro Thr Asp Thr Thr Phe Tyr Lys Arg Thr 
        355                 360                 365             


Tyr Pro Asp Ala Asp Gly Arg Phe Val Gln Glu Ile Ile Leu Arg Glu 
    370                 375                 380                 


Ala Val Ile Leu Asn Phe Gly Pro Ile Ala Glu Glu Asp Ala Glu Gly 
385                 390                 395                 400 


Tyr Val Arg Leu Asn Ser Ala Gln Val Glu Arg Leu Arg Asp Ala Val 
                405                 410                 415     


Val Ser Thr Asn Val Arg His Asp Ala Glu Glu Met Glu Glu Leu Leu 
            420                 425                 430         


Ala Arg Lys Glu Arg Glu Glu Glu Glu Ile Ala Glu Leu Lys Ser Arg 
        435                 440                 445             


Ile Ser Glu Leu Arg Asn Lys Val Ser Leu Thr Ala Glu Glu Glu Glu 
    450                 455                 460                 


Glu Leu Ala Gln Leu Glu Lys Leu Leu Ala Asp Ala Glu Arg Arg His 
465                 470                 475                 480 


His Ala Thr Leu Ser Arg Ile Ala Asp Leu Tyr Ala Cys Gly Arg Gly 
                485                 490                 495     


Ala Arg Glu Ile Thr Leu Ala Ile Lys Arg Pro Glu Phe Arg Tyr Thr 
            500                 505                 510         


Arg Leu His Cys Lys Met Ser Gly Asp Trp Glu Thr Ile Leu Ser Asp 
        515                 520                 525             


Ala Glu Arg Tyr Ser Glu Met Ile Ala Ala Phe Cys Asp Asp Val Ser 
    530                 535                 540                 


Thr Leu Leu Asn Ile Pro Ala Ser Tyr Val Leu Asp Ile Asp Thr Cys 
545                 550                 555                 560 


Cys Gly Ser Leu Leu Ile Asp Phe Thr Val Lys His Ser Gly Asp Leu 
                565                 570                 575     


Asp Asp Glu Gln Leu Gln Asp Leu Val Asn Lys Gly Cys Phe Ser Ala 
            580                 585                 590         


Leu Cys Met Phe Tyr Glu Lys Val Thr Phe Lys Lys Thr Ser Pro Leu 
        595                 600                 605             


Asn Thr Ser Gln Gln Gln Leu Ala Tyr Asp Leu Glu Gln Arg Leu Asn 
    610                 615                 620                 


Gly Pro Ala Pro Ile Ser Gly Met Gly Ile Lys Gln Ile Leu Thr Asp 
625                 630                 635                 640 


Tyr Tyr Asn Ala Asp Gly Thr Leu Asp Glu Glu Phe Ser Asp Glu Val 
                645                 650                 655     


Lys Ala His Lys Asp Tyr Arg Lys Ala Leu Ile Thr Ile Pro Pro Leu 
            660                 665                 670         


Arg Glu Asp Tyr Asp Asp Val Thr Val Arg Gly Pro Phe Glu Gln His 
        675                 680                 685             


Ala Glu Asp Glu Gly Glu Ala Leu Ser Ala Ala Val Pro Val Ser Ile 
    690                 695                 700                 


Met Ala Ser Ser Val Ala Gly Asn Arg Asn Lys Gly Glu Asp Glu Leu 
705                 710                 715                 720 


Ala Gly Ala Thr Gln Thr Ser Ile Asp Leu Thr Asp Thr Gly Ala Ala 
                725                 730                 735     


Thr His Asp Met Thr Glu Ser Ala Pro Ala Met Glu Val His Ser His 
            740                 745                 750         


Asp Met Asn Leu Ser Tyr Asn Pro Gln Pro Ala Val Asp Ala Ala Ala 
        755                 760                 765             


Pro Ala Ala Pro Ala Ala Pro Ser Val Ser Ala Pro Ala Glu Asn Val 
    770                 775                 780                 


Gly Gln Pro Gly Val Pro Asn Thr Asp Ala Gly Ser Leu Ser Arg Ser 
785                 790                 795                 800 


Ser Glu Met Ser Arg Val Asn 
                805         


<210>  3
<211>  723
<212>  PRT
<213>  Leishmania major

<400>  3

Met Gly Cys Cys Asn Ser Lys Glu Thr Lys Lys Pro Lys Glu Glu Val 
1               5                   10                  15      


Leu Gln Arg Glu Ala Gln Tyr Ala Lys Val Ser Ser Glu Thr Asp Ser 
            20                  25                  30          


Val Glu Glu Gln Pro Asn Ile Glu Ala Arg Val Ala Ser Val Val Lys 
        35                  40                  45              


Asp Leu Arg Ala Glu Arg Ile Asp Ala Ile Cys Ala His Ser His Ala 
    50                  55                  60                  


Cys Pro Tyr Arg Tyr Asn Ala Cys Thr Val Val Gly Gly Glu Val Lys 
65                  70                  75                  80  


Phe Tyr Phe Glu Gly Gly Ile Ile Phe Cys Ile Val Lys Glu Gly Ser 
                85                  90                  95      


Trp Tyr Ile Tyr Asn Asp Ser Leu Asp Tyr Glu Ala His Val Thr Val 
            100                 105                 110         


Arg Phe Gly Pro Gly Ser Asn Ile Ala Ala Gly Gln Arg Ala Glu Leu 
        115                 120                 125             


Gly Glu Val Glu Asp Gly Trp Thr Cys Ile Arg Ala Ile Val Tyr Pro 
    130                 135                 140                 


Leu Glu Thr Val His Leu Val Ser Gly Thr Ala Asn Gly Tyr Lys Thr 
145                 150                 155                 160 


His Ile Thr Ile Lys Pro Leu Asn Glu Gln Tyr Arg His Glu Ala Cys 
                165                 170                 175     


Ala Ala Ala Asn Gly Thr Ala Glu Ala Glu Thr Glu Ala Val Arg Ser 
            180                 185                 190         


Leu Val Thr Glu Gly Met Asp Glu Glu Ala Ile Leu His Arg Cys Val 
        195                 200                 205             


Glu Thr Thr Thr Pro Tyr Val Asp Leu Thr Phe Pro Pro Asn Gly Glu 
    210                 215                 220                 


Ala Val Ala Arg Ala Gly Arg Asp Thr Arg Thr Ile Pro Glu Val Ala 
225                 230                 235                 240 


Met Met Arg Pro Thr Gln Tyr Leu Leu Asp Asn Asn Cys Leu Ser Val 
                245                 250                 255     


Asn Asp Ile Cys Gly Pro Val Leu Ala Leu Ser Ile Glu Pro Gly Asn 
            260                 265                 270         


Leu Gly Asp Ser Trp Phe Met Cys Ala Val Ala Ile Met Ala Glu Asn 
        275                 280                 285             


Glu Ala Ala Val Arg Ser Ile Phe Ala Gln Gly Ser Pro Ala Glu Lys 
    290                 295                 300                 


Ala Val Gly Ala Tyr Arg Val Leu Val Asn Lys Asn Gly Trp Trp His 
305                 310                 315                 320 


Thr Leu Ile Met Asp Asp Tyr Leu Pro Thr Phe Asn Arg Met Pro Val 
                325                 330                 335     


Phe Ala Arg Ser Tyr Asp Asn Pro Ala Glu Val Trp Thr Ser Leu Leu 
            340                 345                 350         


Gln Lys Ala Tyr Ala Lys Leu His Gly Ser Tyr Ala Ala Ile Thr Gly 
        355                 360                 365             


Gly Asp Thr Leu Gln Ala Leu Ala Asp Leu Ser Gly Ala Pro Met Cys 
    370                 375                 380                 


Arg Phe Asp Lys Glu Trp Glu Glu Ala Thr Thr Asp Ala Glu Lys Ala 
385                 390                 395                 400 


Asp Thr Leu Ala Asn Ala Leu Val Gln Phe Ser Arg Ser Gly Ala Cys 
                405                 410                 415     


Val Val Leu Ser Thr Pro Gly His Asn Ser Glu Ser Tyr Leu Gly Arg 
            420                 425                 430         


Ser Gln Ala Ser Asp Ala Ala Ala Phe Arg Ala Arg Tyr Lys Lys Val 
        435                 440                 445             


Gly Leu His Val Gly Tyr Thr Tyr Ser Leu Glu Arg Val Val Met Val 
    450                 455                 460                 


Glu Glu Cys Gly Thr Leu Leu Phe Lys Val Arg Asn Pro Trp Arg Ser 
465                 470                 475                 480 


Ser Ser Lys Trp Thr Gly Pro Trp Ser Tyr Gly Ser Arg Glu Trp Asp 
                485                 490                 495     


Glu Asn Gln Asp Ala Cys Leu Leu Cys Gly Ala Gln Lys Asp Pro Gln 
            500                 505                 510         


Asp Gly Ser Phe Trp Met Cys Trp Asp Asp Ala Ser Arg Tyr Phe Asp 
        515                 520                 525             


Gly Gly Gly Val Leu Phe Ala Asn Pro Asp Ala Val Asp Tyr Arg Val 
    530                 535                 540                 


Lys Gly Val Phe Cys Lys Thr Ile Pro Ser Ala Val Leu Glu Ile Thr 
545                 550                 555                 560 


Ala Ser Glu Ser Thr Gln Val Phe Phe Thr Leu Ser Gln Pro Asp Lys 
                565                 570                 575     


Arg Gly Val Asp Arg Lys Glu Gly Ala Ala Leu Phe Ala Pro Ile Met 
            580                 585                 590         


Leu Thr Val Ser Lys Glu Glu Asp Gly Val Gln Arg Val Gln Lys Asn 
        595                 600                 605             


Thr Ser Trp Asn Pro Thr Met Pro Ser Glu Glu Phe Asn Phe Val Val 
    610                 615                 620                 


Gly Arg Asp Val Ser Met Trp Phe Thr Leu Gly Ala Glu Glu Lys Tyr 
625                 630                 635                 640 


Leu Val Val Pro Arg Ile His Ser Lys Gly Val Lys Ser Asp Tyr Asp 
                645                 650                 655     


Arg Pro Tyr Val Ile Ser Ile Met Ser Thr Asp Lys Leu Glu Gly Asn 
            660                 665                 670         


Val Arg Val Glu Ala Lys His Ile His Ser Asp Ser Lys Val Phe Thr 
        675                 680                 685             


Asn Tyr Ile Ala Tyr Asp Ser Glu Glu Leu Pro Ser Val Lys Val Glu 
    690                 695                 700                 


Asn Gln Val Arg Leu Pro Gly Lys Ala Pro Val Thr Tyr Val Ser Ala 
705                 710                 715                 720 


Thr Val Ile 
            


<210>  4
<211>  149
<212>  PRT
<213>  Leishmania major

<400>  4

Met Ala Asp Gln Leu Ser Asn Glu Gln Ile Ser Glu Phe Lys Glu Ala 
1               5                   10                  15      


Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu 
            20                  25                  30          


Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu 
        35                  40                  45              


Leu Gln Asp Met Ile Asn Glu Val Asp Gln Asp Gly Ser Gly Thr Ile 
    50                  55                  60                  


Asp Phe Pro Glu Phe Leu Thr Leu Met Ala Arg Lys Met Gln Asp Ser 
65                  70                  75                  80  


Asp Ser Glu Glu Glu Ile Lys Glu Ala Phe Arg Val Phe Asp Lys Asp 
                85                  90                  95      


Gly Asn Gly Phe Ile Ser Ala Ala Glu Leu Arg His Val Met Thr Asn 
            100                 105                 110         


Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu 
        115                 120                 125             


Ala Asp Val Asp Gly Asp Gly Gln Ile Asn Tyr Glu Glu Phe Val Lys 
    130                 135                 140                 


Met Met Met Ser Lys 
145                 


<210>  5
<211>  453
<212>  PRT
<213>  Leishmania major

<400>  5

Met Ala Thr Ile Met Ser Glu His Val Val Lys Met Ala Lys Glu Phe 
1               5                   10                  15      


Val Asn Phe Met Asn Gln Ala Ile Thr Pro Phe His Ala Val Gln Thr 
            20                  25                  30          


Val Ala Lys Met Leu Lys Asp Ala Gly Tyr Thr Gln Leu His Glu Glu 
        35                  40                  45              


Lys Val Trp Pro Glu Leu Thr Pro Gly Gly Lys Tyr Tyr Leu Met Arg 
    50                  55                  60                  


Asn Gly Thr Ser Ile Ile Ala Phe Ser Val Gly Gly Lys Phe Asp Pro 
65                  70                  75                  80  


Met Asn Gly Val Lys Ile Val Gly Ala His Thr Asp Ser Pro Asn Phe 
                85                  90                  95      


Leu Leu Lys Pro Arg Thr Lys Ser Thr Ala Ala Asp Tyr Glu Arg Val 
            100                 105                 110         


Ala Val Gln Cys Tyr Gly Gly Gly Leu Trp His Ser Trp Phe Asp Arg 
        115                 120                 125             


Asp Leu Thr Val Ala Gly Arg Val Met Ile Ser Arg Glu Arg Leu Glu 
    130                 135                 140                 


Gln Lys Ile Ile Lys Ile Asp Lys Pro Ile Met Arg Ile Pro Asn Leu 
145                 150                 155                 160 


Ala Ile His Leu Thr Ser Ala Lys Asp Arg Glu Ser Phe Ser Pro Asn 
                165                 170                 175     


Lys Glu Ser His Leu Ile Pro Ile Ile Ser Thr Gln Ile Ala Ala Arg 
            180                 185                 190         


Ile Ala Glu Cys Asp Asp Lys Asp Ala Ser Asn Pro Asn His Cys Val 
        195                 200                 205             


Ser Leu Ile Lys Ala Ile Ala Ser Val Ala Glu Cys Asn Pro Asp Glu 
    210                 215                 220                 


Ile Val Asp Phe Asp Leu Ser Val Ile Asp Thr Gln Pro Ala Val Ile 
225                 230                 235                 240 


Gly Gly Ile His Asp Glu Phe Ile Phe Ser Pro Arg Leu Asp Asn Leu 
                245                 250                 255     


Ile Ser Cys Tyr Cys Ala Val Lys Ala Ile Ile Glu Ala Asp Ala Leu 
            260                 265                 270         


Glu Asn Asp Thr Met Met Arg Met Val Cys Leu Phe Asp His Glu Glu 
        275                 280                 285             


Cys Gly Ser Ser Ser Ser Gln Gly Ala Ala Gly Ser Leu Val Pro Asp 
    290                 295                 300                 


Val Ile Glu His Ile Val Ser Asn Lys Thr Leu Arg Ala Thr Leu Val 
305                 310                 315                 320 


Ala Asn Ser Phe Leu Leu Ser Val Asp Gly Ala His Gly Cys His Pro 
                325                 330                 335     


Asn Tyr Ala Asp Lys His Glu Asn Ala His Arg Pro Ala Leu His Gly 
            340                 345                 350         


Gly Pro Val Ile Lys Tyr Asn Ala Asn Ala Arg Tyr Ala Thr Asn Gly 
        355                 360                 365             


Leu Thr Ala Ala Val Val Lys Asp Val Ala Lys Lys Ala Ala Ile Pro 
    370                 375                 380                 


Ile Gln Glu Phe Val Val Arg Asn Asp Ser Pro Cys Gly Ser Thr Ile 
385                 390                 395                 400 


Gly Pro Ile Leu Ser Ser Leu Ser Gly Ile Lys Thr Ala Asp Ile Gly 
                405                 410                 415     


Asn Pro Met Ile Ser Met His Ser Ile Arg Glu Met Cys Gly Thr Val 
            420                 425                 430         


Asp Val Tyr Tyr Met Thr Lys Leu Ile Glu Ser Phe Phe Ile Asn Tyr 
        435                 440                 445             


Gln Asn Pro His Glu 
    450             


