                         SEQUENCE LISTING

<110>  BIO-TECHNE CORPORATION
       Goetz, Christine
       Hammerbeck, Chris
       Valley, Christopher Carlin
       Bonnevier, Jody
       Aggeler, Birte
       Wang, Jinghua
       Wu, Guoping
       Kalabokis, Vassilios
       Bazan, Jose Fernando
 
<120>  COMPOUNDS THAT MODULATE THE INTERACTION OF VISTA AND VSIG3 AND 
       METHODS OF MAKING AND USING

<130>  0541.000007WO02

<150>  US 62/625,594
<151>  2018-02-02

<150>  US 62/627,478
<151>  2018-02-07

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  431
<212>  PRT
<213>  homo sapiens

<400>  1

Met Thr Ser Gln Arg Ser Pro Leu Ala Pro Leu Leu Leu Leu Ser Leu 
1               5                   10                  15      


His Gly Val Ala Ala Ser Leu Glu Val Ser Glu Ser Pro Gly Ser Ile 
            20                  25                  30          


Gln Val Ala Arg Gly Gln Pro Ala Val Leu Pro Cys Thr Phe Thr Thr 
        35                  40                  45              


Ser Ala Ala Leu Ile Asn Leu Asn Val Ile Trp Met Val Thr Pro Leu 
    50                  55                  60                  


Ser Asn Ala Asn Gln Pro Glu Gln Val Ile Leu Tyr Gln Gly Gly Gln 
65                  70                  75                  80  


Met Phe Asp Gly Ala Pro Arg Phe His Gly Arg Val Gly Phe Thr Gly 
                85                  90                  95      


Thr Met Pro Ala Thr Asn Val Ser Ile Phe Ile Asn Asn Thr Gln Leu 
            100                 105                 110         


Ser Asp Thr Gly Thr Tyr Gln Cys Leu Val Asn Asn Leu Pro Asp Ile 
        115                 120                 125             


Gly Gly Arg Asn Ile Gly Val Thr Gly Leu Thr Val Leu Val Pro Pro 
    130                 135                 140                 


Ser Ala Pro His Cys Gln Ile Gln Gly Ser Gln Asp Ile Gly Ser Asp 
145                 150                 155                 160 


Val Ile Leu Leu Cys Ser Ser Glu Glu Gly Ile Pro Arg Pro Thr Tyr 
                165                 170                 175     


Leu Trp Glu Lys Leu Asp Asn Thr Leu Lys Leu Pro Pro Thr Ala Thr 
            180                 185                 190         


Gln Asp Gln Val Gln Gly Thr Val Thr Ile Arg Asn Ile Ser Ala Leu 
        195                 200                 205             


Ser Ser Gly Leu Tyr Gln Cys Val Ala Ser Asn Ala Ile Gly Thr Ser 
    210                 215                 220                 


Thr Cys Leu Leu Asp Leu Gln Val Ile Ser Pro Gln Pro Arg Asn Ile 
225                 230                 235                 240 


Gly Leu Ile Ala Gly Ala Ile Gly Thr Gly Ala Val Ile Ile Ile Phe 
                245                 250                 255     


Cys Ile Ala Leu Ile Leu Gly Ala Phe Phe Tyr Trp Arg Ser Lys Asn 
            260                 265                 270         


Lys Glu Glu Glu Glu Glu Glu Ile Pro Asn Glu Ile Arg Glu Asp Asp 
        275                 280                 285             


Leu Pro Pro Lys Cys Ser Ser Ala Lys Ala Phe His Thr Glu Ile Ser 
    290                 295                 300                 


Ser Ser Asp Asn Asn Thr Leu Thr Ser Ser Asn Ala Tyr Asn Ser Arg 
305                 310                 315                 320 


Tyr Trp Ser Asn Asn Pro Lys Val His Arg Asn Thr Glu Ser Val Ser 
                325                 330                 335     


His Phe Ser Asp Leu Gly Gln Ser Phe Ser Phe His Ser Gly Asn Ala 
            340                 345                 350         


Asn Ile Pro Ser Ile Tyr Ala Asn Gly Thr His Leu Val Pro Gly Gln 
        355                 360                 365             


His Lys Thr Leu Val Val Thr Ala Asn Arg Gly Ser Ser Pro Gln Val 
    370                 375                 380                 


Met Ser Arg Ser Asn Gly Ser Val Ser Arg Lys Pro Arg Pro Pro His 
385                 390                 395                 400 


Thr His Ser Tyr Thr Ile Ser His Ala Thr Leu Glu Arg Ile Gly Ala 
                405                 410                 415     


Val Pro Val Met Val Pro Ala Gln Ser Arg Ala Gly Ser Leu Val 
            420                 425                 430     


<210>  2
<211>  245
<212>  PRT
<213>  homo sapiens

<400>  2

Met Thr Ser Gln Arg Ser Pro Leu Ala Pro Leu Leu Leu Leu Ser Leu 
1               5                   10                  15      


His Gly Val Ala Ala Ser Leu Glu Val Ser Glu Ser Pro Gly Ser Ile 
            20                  25                  30          


Gln Val Ala Arg Gly Gln Thr Ala Val Leu Pro Cys Thr Phe Thr Thr 
        35                  40                  45              


Ser Ala Ala Leu Ile Asn Leu Asn Val Ile Trp Met Val Thr Pro Leu 
    50                  55                  60                  


Ser Asn Ala Asn Gln Pro Glu Gln Val Ile Leu Tyr Gln Gly Gly Gln 
65                  70                  75                  80  


Met Phe Asp Gly Ala Pro Arg Phe His Gly Arg Val Gly Phe Thr Gly 
                85                  90                  95      


Thr Met Pro Ala Thr Asn Val Ser Ile Phe Ile Asn Asn Thr Gln Leu 
            100                 105                 110         


Ser Asp Thr Gly Thr Tyr Gln Cys Leu Val Asn Asn Leu Pro Asp Ile 
        115                 120                 125             


Gly Gly Arg Asn Ile Gly Val Thr Gly Leu Thr Val Leu Val Pro Pro 
    130                 135                 140                 


Ser Ala Pro His Cys Gln Ile Gln Gly Ser Gln Asp Ile Gly Ser Asp 
145                 150                 155                 160 


Val Ile Leu Leu Cys Ser Ser Glu Glu Gly Ile Pro Arg Pro Thr Tyr 
                165                 170                 175     


Leu Trp Glu Lys Leu Asp Asn Thr Leu Lys Leu Pro Pro Thr Ala Thr 
            180                 185                 190         


Gln Asp Gln Val Gln Gly Thr Val Thr Ile Arg Asn Ile Ser Ala Leu 
        195                 200                 205             


Ser Ser Gly Leu Tyr Gln Cys Val Ala Ser Asn Ala Ile Gly Thr Ser 
    210                 215                 220                 


Thr Cys Leu Leu Asp Leu Gln Val Ile Ser Pro Gln Pro Arg Asn Ile 
225                 230                 235                 240 


Gly Leu Ile Ala Gly 
                245 


<210>  3
<211>  460
<212>  PRT
<213>  homo sapiens

<400>  3

Leu Glu Val Ser Glu Ser Pro Gly Ser Ile Gln Val Ala Arg Gly Gln 
1               5                   10                  15      


Thr Ala Val Leu Pro Cys Thr Phe Thr Thr Ser Ala Ala Leu Ile Asn 
            20                  25                  30          


Leu Asn Val Ile Trp Met Val Thr Pro Leu Ser Asn Ala Asn Gln Pro 
        35                  40                  45              


Glu Gln Val Ile Leu Tyr Gln Gly Gly Gln Met Phe Asp Gly Ala Pro 
    50                  55                  60                  


Arg Phe His Gly Arg Val Gly Phe Thr Gly Thr Met Pro Ala Thr Asn 
65                  70                  75                  80  


Val Ser Ile Phe Ile Asn Asn Thr Gln Leu Ser Asp Thr Gly Thr Tyr 
                85                  90                  95      


Gln Cys Leu Val Asn Asn Leu Pro Asp Ile Gly Gly Arg Asn Ile Gly 
            100                 105                 110         


Val Thr Gly Leu Thr Val Leu Val Pro Pro Ser Ala Pro His Cys Gln 
        115                 120                 125             


Ile Gln Gly Ser Gln Asp Ile Gly Ser Asp Val Ile Leu Leu Cys Ser 
    130                 135                 140                 


Ser Glu Glu Gly Ile Pro Arg Pro Thr Tyr Leu Trp Glu Lys Leu Asp 
145                 150                 155                 160 


Asn Thr Leu Lys Leu Pro Pro Thr Ala Thr Gln Asp Gln Val Gln Gly 
                165                 170                 175     


Thr Val Thr Ile Arg Asn Ile Ser Ala Leu Ser Ser Gly Leu Tyr Gln 
            180                 185                 190         


Cys Val Ala Ser Asn Ala Ile Gly Thr Ser Thr Cys Leu Leu Asp Leu 
        195                 200                 205             


Gln Val Ile Ser Pro Gln Pro Arg Asn Ile Gly Leu Ile Ala Gly Ile 
    210                 215                 220                 


Glu Gly Arg Met Asp Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
225                 230                 235                 240 


Pro Cys Pro Ala Pro Glu Ala Glu Gly Ala Pro Ser Val Phe Leu Phe 
                245                 250                 255     


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
            260                 265                 270         


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
        275                 280                 285             


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
    290                 295                 300                 


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
305                 310                 315                 320 


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
                325                 330                 335     


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
            340                 345                 350         


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
        355                 360                 365             


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
    370                 375                 380                 


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
385                 390                 395                 400 


Glu Asn Asn Tyr Lys Ala Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
                405                 410                 415     


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
            420                 425                 430         


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
        435                 440                 445             


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    450                 455                 460 


<210>  4
<211>  311
<212>  PRT
<213>  homo sapiens

<400>  4

Met Gly Val Pro Thr Ala Leu Glu Ala Gly Ser Trp Arg Trp Gly Ser 
1               5                   10                  15      


Leu Leu Phe Ala Leu Phe Leu Ala Ala Ser Leu Gly Pro Val Ala Ala 
            20                  25                  30          


Phe Lys Val Ala Thr Pro Tyr Ser Leu Tyr Val Cys Pro Glu Gly Gln 
        35                  40                  45              


Asn Val Thr Leu Thr Cys Arg Leu Leu Gly Pro Val Asp Lys Gly His 
    50                  55                  60                  


Asp Val Thr Phe Tyr Lys Thr Trp Tyr Arg Ser Ser Arg Gly Glu Val 
65                  70                  75                  80  


Gln Thr Cys Ser Glu Arg Arg Pro Ile Arg Asn Leu Thr Phe Gln Asp 
                85                  90                  95      


Leu His Leu His His Gly Gly His Gln Ala Ala Asn Thr Ser His Asp 
            100                 105                 110         


Leu Ala Gln Arg His Gly Leu Glu Ser Ala Ser Asp His His Gly Asn 
        115                 120                 125             


Phe Ser Ile Thr Met Arg Asn Leu Thr Leu Leu Asp Ser Gly Leu Tyr 
    130                 135                 140                 


Cys Cys Leu Val Val Glu Ile Arg His His His Ser Glu His Arg Val 
145                 150                 155                 160 


His Gly Ala Met Glu Leu Gln Val Gln Thr Gly Lys Asp Ala Pro Ser 
                165                 170                 175     


Asn Cys Val Val Tyr Pro Ser Ser Ser Gln Asp Ser Glu Asn Ile Thr 
            180                 185                 190         


Ala Ala Ala Leu Ala Thr Gly Ala Cys Ile Val Gly Ile Leu Cys Leu 
        195                 200                 205             


Pro Leu Ile Leu Leu Leu Val Tyr Lys Gln Arg Gln Ala Ala Ser Asn 
    210                 215                 220                 


Arg Arg Ala Gln Glu Leu Val Arg Met Asp Ser Asn Ile Gln Gly Ile 
225                 230                 235                 240 


Glu Asn Pro Gly Phe Glu Ala Ser Pro Pro Ala Gln Gly Ile Pro Glu 
                245                 250                 255     


Ala Lys Val Arg His Pro Leu Ser Tyr Val Ala Gln Arg Gln Pro Ser 
            260                 265                 270         


Glu Ser Gly Arg His Leu Leu Ser Glu Pro Ser Thr Pro Leu Ser Pro 
        275                 280                 285             


Pro Gly Pro Gly Asp Val Phe Phe Pro Ser Leu Asp Pro Val Pro Asp 
    290                 295                 300                 


Ser Pro Asn Phe Glu Val Ile 
305                 310     


<210>  5
<211>  198
<212>  PRT
<213>  homo sapiens

<400>  5

Met Gly Val Pro Thr Ala Leu Glu Ala Gly Ser Trp Arg Trp Gly Ser 
1               5                   10                  15      


Leu Leu Phe Ala Leu Phe Leu Ala Ala Ser Leu Gly Pro Val Ala Ala 
            20                  25                  30          


Phe Lys Val Ala Thr Pro Tyr Ser Leu Tyr Val Cys Pro Glu Gly Gln 
        35                  40                  45              


Asn Val Thr Leu Thr Cys Arg Leu Leu Gly Pro Val Asp Lys Gly His 
    50                  55                  60                  


Asp Val Thr Phe Tyr Lys Thr Trp Tyr Arg Ser Ser Arg Gly Glu Val 
65                  70                  75                  80  


Gln Thr Cys Ser Glu Arg Arg Pro Ile Arg Asn Leu Thr Phe Gln Asp 
                85                  90                  95      


Leu His Leu His His Gly Gly His Gln Ala Ala Asn Thr Ser His Asp 
            100                 105                 110         


Leu Ala Gln Arg His Gly Leu Glu Ser Ala Ser Asp His His Gly Asn 
        115                 120                 125             


Phe Ser Ile Thr Met Arg Asn Leu Thr Leu Leu Asp Ser Gly Leu Tyr 
    130                 135                 140                 


Cys Cys Leu Val Val Glu Ile Arg His His His Ser Glu His Arg Val 
145                 150                 155                 160 


His Gly Ala Met Glu Leu Gln Val Gln Thr Gly Lys Asp Ala Pro Ser 
                165                 170                 175     


Asn Cys Val Val Tyr Pro Ser Ser Ser Gln Glu Ser Glu Asn Ile Thr 
            180                 185                 190         


Ala Ala Ile Glu Gly Arg 
        195             


<210>  6
<211>  399
<212>  PRT
<213>  homo sapiens

<400>  6

Phe Lys Val Ala Thr Pro Tyr Ser Leu Tyr Val Cys Pro Glu Gly Gln 
1               5                   10                  15      


Asn Val Thr Leu Thr Cys Arg Leu Leu Gly Pro Val Asp Lys Gly His 
            20                  25                  30          


Asp Val Thr Phe Tyr Lys Thr Trp Tyr Arg Ser Ser Arg Gly Glu Val 
        35                  40                  45              


Gln Thr Cys Ser Glu Arg Arg Pro Ile Arg Asn Leu Thr Phe Gln Asp 
    50                  55                  60                  


Leu His Leu His His Gly Gly His Gln Ala Ala Asn Thr Ser His Asp 
65                  70                  75                  80  


Leu Ala Gln Arg His Gly Leu Glu Ser Ala Ser Asp His His Gly Asn 
                85                  90                  95      


Phe Ser Ile Thr Met Arg Asn Leu Thr Leu Leu Asp Ser Gly Leu Tyr 
            100                 105                 110         


Cys Cys Leu Val Val Glu Ile Arg His His His Ser Glu His Arg Val 
        115                 120                 125             


His Gly Ala Met Glu Leu Gln Val Gln Thr Gly Lys Asp Ala Pro Ser 
    130                 135                 140                 


Asn Cys Val Val Tyr Pro Ser Ser Ser Gln Glu Ser Glu Asn Ile Thr 
145                 150                 155                 160 


Ala Ala Ile Glu Gly Arg Met Asp Pro Lys Ser Cys Asp Lys Thr His 
                165                 170                 175     


Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Glu Gly Ala Pro Ser Val 
            180                 185                 190         


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
        195                 200                 205             


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
    210                 215                 220                 


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
225                 230                 235                 240 


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
                245                 250                 255     


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
            260                 265                 270         


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
        275                 280                 285             


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
    290                 295                 300                 


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
305                 310                 315                 320 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
                325                 330                 335     


Gly Gln Pro Glu Asn Asn Tyr Lys Ala Thr Pro Pro Val Leu Asp Ser 
            340                 345                 350         


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
        355                 360                 365             


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
    370                 375                 380                 


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
385                 390                 395                 


<210>  7
<211>  772
<212>  PRT
<213>  Artificial

<220>
<223>  VISTA(1-195)-COMP-AlkPhos-Flag/His fusion

<400>  7

Met Gly Val Pro Thr Ala Leu Glu Ala Gly Ser Trp Arg Trp Gly Ser 
1               5                   10                  15      


Leu Leu Phe Ala Leu Phe Leu Ala Ala Ser Leu Gly Pro Val Ala Ala 
            20                  25                  30          


Phe Lys Val Ala Thr Pro Tyr Ser Leu Tyr Val Cys Pro Glu Gly Gln 
        35                  40                  45              


Asn Val Thr Leu Thr Cys Arg Leu Leu Gly Pro Val Asp Lys Gly His 
    50                  55                  60                  


Asp Val Thr Phe Tyr Lys Thr Trp Tyr Arg Ser Ser Arg Gly Glu Val 
65                  70                  75                  80  


Gln Thr Cys Ser Glu Arg Arg Pro Ile Arg Asn Leu Thr Phe Gln Asp 
                85                  90                  95      


Leu His Leu His His Gly Gly His Gln Ala Ala Asn Thr Ser His Asp 
            100                 105                 110         


Leu Ala Gln Arg His Gly Leu Glu Ser Ala Ser Asp His His Gly Asn 
        115                 120                 125             


Phe Ser Ile Thr Met Arg Asn Leu Thr Leu Leu Asp Ser Gly Leu Tyr 
    130                 135                 140                 


Cys Cys Leu Val Val Glu Ile Arg His His His Ser Glu His Arg Val 
145                 150                 155                 160 


His Gly Ala Met Glu Leu Gln Val Gln Thr Gly Lys Asp Ala Pro Ser 
                165                 170                 175     


Asn Cys Val Val Tyr Pro Ser Ser Ser Gln Glu Ser Glu Asn Ile Thr 
            180                 185                 190         


Ala Ala Gly Ser Glu Asn Leu Tyr Phe Gln Gly Asn Ser Gly Gly Gly 
        195                 200                 205             


Ser Gly Gly Gly Thr Gly Gly Gly Asp Leu Ala Pro Gln Met Leu Arg 
    210                 215                 220                 


Glu Leu Gln Glu Thr Asn Ala Ala Leu Gln Asp Val Arg Glu Leu Leu 
225                 230                 235                 240 


Arg His Arg Val Lys Glu Ile Thr Phe Leu Lys Asn Thr Val Met Glu 
                245                 250                 255     


Cys Asp Ala Cys Gly Leu Asp Arg Asn Leu Pro Pro Leu Ala Pro Leu 
            260                 265                 270         


Gly Pro Ile Ile Pro Val Glu Glu Glu Asn Pro Asp Phe Trp Asn Arg 
        275                 280                 285             


Glu Ala Ala Glu Ala Leu Gly Ala Ala Lys Lys Leu Gln Pro Ala Gln 
    290                 295                 300                 


Thr Ala Ala Lys Asn Leu Ile Ile Phe Leu Gly Asp Gly Met Gly Val 
305                 310                 315                 320 


Ser Thr Val Thr Ala Ala Arg Ile Leu Lys Gly Gln Lys Lys Asp Lys 
                325                 330                 335     


Leu Gly Pro Glu Ile Pro Leu Ala Met Asp Arg Phe Pro Tyr Val Ala 
            340                 345                 350         


Leu Ser Lys Thr Tyr Asn Val Asp Lys His Val Pro Asp Ser Gly Ala 
        355                 360                 365             


Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Gly Asn Phe Gln Thr Ile 
    370                 375                 380                 


Gly Leu Ser Ala Ala Ala Arg Phe Asn Gln Cys Asn Thr Thr Arg Gly 
385                 390                 395                 400 


Asn Glu Val Ile Ser Val Met Asn Arg Ala Lys Lys Ala Gly Lys Ser 
                405                 410                 415     


Val Gly Val Val Thr Thr Thr Arg Val Gln His Ala Ser Pro Ala Gly 
            420                 425                 430         


Thr Tyr Ala His Thr Val Asn Arg Asn Trp Tyr Ser Asp Ala Asp Val 
        435                 440                 445             


Pro Ala Ser Ala Arg Gln Glu Gly Cys Gln Asp Ile Ala Thr Gln Leu 
    450                 455                 460                 


Ile Ser Asn Met Asp Ile Asp Val Ile Leu Gly Gly Gly Arg Lys Tyr 
465                 470                 475                 480 


Met Phe Arg Met Gly Thr Pro Asp Pro Glu Tyr Pro Asp Asp Tyr Ser 
                485                 490                 495     


Gln Gly Gly Thr Arg Leu Asp Gly Lys Asn Leu Val Gln Glu Trp Leu 
            500                 505                 510         


Ala Lys Arg Gln Gly Ala Arg Tyr Val Trp Asn Arg Thr Glu Leu Met 
        515                 520                 525             


Gln Ala Ser Leu Asp Pro Ser Val Thr His Leu Met Gly Leu Phe Glu 
    530                 535                 540                 


Pro Gly Asp Met Lys Tyr Glu Ile His Arg Asp Ser Thr Leu Asp Pro 
545                 550                 555                 560 


Ser Leu Met Glu Met Thr Glu Ala Ala Leu Arg Leu Leu Ser Arg Asn 
                565                 570                 575     


Pro Arg Gly Phe Phe Leu Phe Val Glu Gly Gly Arg Ile Asp His Gly 
            580                 585                 590         


His His Glu Ser Arg Ala Tyr Arg Ala Leu Thr Glu Thr Ile Met Phe 
        595                 600                 605             


Asp Asp Ala Ile Glu Arg Ala Gly Gln Leu Thr Ser Glu Glu Asp Thr 
    610                 615                 620                 


Leu Ser Leu Val Thr Ala Asp His Ser His Val Phe Ser Phe Gly Gly 
625                 630                 635                 640 


Tyr Pro Leu Arg Gly Ser Ser Ile Phe Gly Leu Ala Pro Gly Lys Ala 
                645                 650                 655     


Arg Asp Arg Lys Ala Tyr Thr Val Leu Leu Tyr Gly Asn Gly Pro Gly 
            660                 665                 670         


Tyr Val Leu Lys Asp Gly Ala Arg Pro Asp Val Thr Glu Ser Glu Ser 
        675                 680                 685             


Gly Ser Pro Glu Tyr Arg Gln Gln Ser Ala Val Pro Leu Asp Glu Glu 
    690                 695                 700                 


Thr His Ala Gly Glu Asp Val Ala Val Phe Ala Arg Gly Pro Gln Ala 
705                 710                 715                 720 


His Leu Val His Gly Val Gln Glu Gln Thr Phe Ile Ala His Val Met 
                725                 730                 735     


Ala Phe Ala Ala Cys Leu Glu Pro Tyr Thr Ala Cys Asp Leu Ala Pro 
            740                 745                 750         


Pro Ala Gly Thr Thr Asp Asp Tyr Lys Asp Asp Asp Asp Lys His His 
        755                 760                 765             


His His His His 
    770         


<210>  8

<400>  8
000

<210>  9
<211>  822
<212>  PRT
<213>  Artificial

<220>
<223>  VSIG3(1-245)-COMP-AlkPhos-Flag/His fusion

<400>  9

Met Thr Ser Gln Arg Ser Pro Leu Ala Pro Leu Leu Leu Leu Ser Leu 
1               5                   10                  15      


His Gly Val Ala Ala Ser Leu Glu Val Ser Glu Ser Pro Gly Ser Ile 
            20                  25                  30          


Gln Val Ala Arg Gly Gln Pro Ala Val Leu Pro Cys Thr Phe Thr Thr 
        35                  40                  45              


Ser Ala Ala Leu Ile Asn Leu Asn Val Ile Trp Met Val Thr Pro Leu 
    50                  55                  60                  


Ser Asn Ala Asn Gln Pro Glu Gln Val Ile Leu Tyr Gln Gly Gly Gln 
65                  70                  75                  80  


Met Phe Asp Gly Ala Pro Arg Phe His Gly Arg Val Gly Phe Thr Gly 
                85                  90                  95      


Thr Met Pro Ala Thr Asn Val Ser Ile Phe Ile Asn Asn Thr Gln Leu 
            100                 105                 110         


Ser Asp Thr Gly Thr Tyr Gln Cys Leu Val Asn Asn Leu Pro Asp Ile 
        115                 120                 125             


Gly Gly Arg Asn Ile Gly Val Thr Gly Leu Thr Val Leu Val Pro Pro 
    130                 135                 140                 


Ser Ala Pro His Cys Gln Ile Gln Gly Ser Gln Asp Ile Gly Ser Asp 
145                 150                 155                 160 


Val Ile Leu Leu Cys Ser Ser Glu Glu Gly Ile Pro Arg Pro Thr Tyr 
                165                 170                 175     


Leu Trp Glu Lys Leu Asp Asn Thr Leu Lys Leu Pro Pro Thr Ala Thr 
            180                 185                 190         


Gln Asp Gln Val Gln Gly Thr Val Thr Ile Arg Asn Ile Ser Ala Leu 
        195                 200                 205             


Ser Ser Gly Leu Tyr Gln Cys Val Ala Ser Asn Ala Ile Gly Thr Ser 
    210                 215                 220                 


Thr Cys Leu Leu Asp Leu Gln Val Ile Ser Pro Gln Pro Arg Asn Ile 
225                 230                 235                 240 


Gly Leu Ile Ala Gly Ser Glu Asn Leu Tyr Phe Gln Gly Asn Ser Gly 
                245                 250                 255     


Gly Gly Ser Gly Gly Gly Thr Gly Gly Gly Asp Leu Ala Pro Gln Met 
            260                 265                 270         


Leu Arg Glu Leu Gln Glu Thr Asn Ala Ala Leu Gln Asp Val Arg Glu 
        275                 280                 285             


Leu Leu Arg His Arg Val Lys Glu Ile Thr Phe Leu Lys Asn Thr Val 
    290                 295                 300                 


Met Glu Cys Asp Ala Cys Gly Leu Asp Arg Asn Leu Pro Pro Leu Ala 
305                 310                 315                 320 


Pro Leu Gly Pro Ile Ile Pro Val Glu Glu Glu Asn Pro Asp Phe Trp 
                325                 330                 335     


Asn Arg Glu Ala Ala Glu Ala Leu Gly Ala Ala Lys Lys Leu Gln Pro 
            340                 345                 350         


Ala Gln Thr Ala Ala Lys Asn Leu Ile Ile Phe Leu Gly Asp Gly Met 
        355                 360                 365             


Gly Val Ser Thr Val Thr Ala Ala Arg Ile Leu Lys Gly Gln Lys Lys 
    370                 375                 380                 


Asp Lys Leu Gly Pro Glu Ile Pro Leu Ala Met Asp Arg Phe Pro Tyr 
385                 390                 395                 400 


Val Ala Leu Ser Lys Thr Tyr Asn Val Asp Lys His Val Pro Asp Ser 
                405                 410                 415     


Gly Ala Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Gly Asn Phe Gln 
            420                 425                 430         


Thr Ile Gly Leu Ser Ala Ala Ala Arg Phe Asn Gln Cys Asn Thr Thr 
        435                 440                 445             


Arg Gly Asn Glu Val Ile Ser Val Met Asn Arg Ala Lys Lys Ala Gly 
    450                 455                 460                 


Lys Ser Val Gly Val Val Thr Thr Thr Arg Val Gln His Ala Ser Pro 
465                 470                 475                 480 


Ala Gly Thr Tyr Ala His Thr Val Asn Arg Asn Trp Tyr Ser Asp Ala 
                485                 490                 495     


Asp Val Pro Ala Ser Ala Arg Gln Glu Gly Cys Gln Asp Ile Ala Thr 
            500                 505                 510         


Gln Leu Ile Ser Asn Met Asp Ile Asp Val Ile Leu Gly Gly Gly Arg 
        515                 520                 525             


Lys Tyr Met Phe Arg Met Gly Thr Pro Asp Pro Glu Tyr Pro Asp Asp 
    530                 535                 540                 


Tyr Ser Gln Gly Gly Thr Arg Leu Asp Gly Lys Asn Leu Val Gln Glu 
545                 550                 555                 560 


Trp Leu Ala Lys Arg Gln Gly Ala Arg Tyr Val Trp Asn Arg Thr Glu 
                565                 570                 575     


Leu Met Gln Ala Ser Leu Asp Pro Ser Val Thr His Leu Met Gly Leu 
            580                 585                 590         


Phe Glu Pro Gly Asp Met Lys Tyr Glu Ile His Arg Asp Ser Thr Leu 
        595                 600                 605             


Asp Pro Ser Leu Met Glu Met Thr Glu Ala Ala Leu Arg Leu Leu Ser 
    610                 615                 620                 


Arg Asn Pro Arg Gly Phe Phe Leu Phe Val Glu Gly Gly Arg Ile Asp 
625                 630                 635                 640 


His Gly His His Glu Ser Arg Ala Tyr Arg Ala Leu Thr Glu Thr Ile 
                645                 650                 655     


Met Phe Asp Asp Ala Ile Glu Arg Ala Gly Gln Leu Thr Ser Glu Glu 
            660                 665                 670         


Asp Thr Leu Ser Leu Val Thr Ala Asp His Ser His Val Phe Ser Phe 
        675                 680                 685             


Gly Gly Tyr Pro Leu Arg Gly Ser Ser Ile Phe Gly Leu Ala Pro Gly 
    690                 695                 700                 


Lys Ala Arg Asp Arg Lys Ala Tyr Thr Val Leu Leu Tyr Gly Asn Gly 
705                 710                 715                 720 


Pro Gly Tyr Val Leu Lys Asp Gly Ala Arg Pro Asp Val Thr Glu Ser 
                725                 730                 735     


Glu Ser Gly Ser Pro Glu Tyr Arg Gln Gln Ser Ala Val Pro Leu Asp 
            740                 745                 750         


Glu Glu Thr His Ala Gly Glu Asp Val Ala Val Phe Ala Arg Gly Pro 
        755                 760                 765             


Gln Ala His Leu Val His Gly Val Gln Glu Gln Thr Phe Ile Ala His 
    770                 775                 780                 


Val Met Ala Phe Ala Ala Cys Leu Glu Pro Tyr Thr Ala Cys Asp Leu 
785                 790                 795                 800 


Ala Pro Pro Ala Gly Thr Thr Asp Asp Tyr Lys Asp Asp Asp Asp Lys 
                805                 810                 815     


His His His His His His 
            820         


<210>  10
<211>  330
<212>  PRT
<213>  Artificial

<220>
<223>  VSIG3(1-245)-COMP-His fusion

<400>  10

Met Thr Ser Gln Arg Ser Pro Leu Ala Pro Leu Leu Leu Leu Ser Leu 
1               5                   10                  15      


His Gly Val Ala Ala Ser Leu Glu Val Ser Glu Ser Pro Gly Ser Ile 
            20                  25                  30          


Gln Val Ala Arg Gly Gln Pro Ala Val Leu Pro Cys Thr Phe Thr Thr 
        35                  40                  45              


Ser Ala Ala Leu Ile Asn Leu Asn Val Ile Trp Met Val Thr Pro Leu 
    50                  55                  60                  


Ser Asn Ala Asn Gln Pro Glu Gln Val Ile Leu Tyr Gln Gly Gly Gln 
65                  70                  75                  80  


Met Phe Asp Gly Ala Pro Arg Phe His Gly Arg Val Gly Phe Thr Gly 
                85                  90                  95      


Thr Met Pro Ala Thr Asn Val Ser Ile Phe Ile Asn Asn Thr Gln Leu 
            100                 105                 110         


Ser Asp Thr Gly Thr Tyr Gln Cys Leu Val Asn Asn Leu Pro Asp Ile 
        115                 120                 125             


Gly Gly Arg Asn Ile Gly Val Thr Gly Leu Thr Val Leu Val Pro Pro 
    130                 135                 140                 


Ser Ala Pro His Cys Gln Ile Gln Gly Ser Gln Asp Ile Gly Ser Asp 
145                 150                 155                 160 


Val Ile Leu Leu Cys Ser Ser Glu Glu Gly Ile Pro Arg Pro Thr Tyr 
                165                 170                 175     


Leu Trp Glu Lys Leu Asp Asn Thr Leu Lys Leu Pro Pro Thr Ala Thr 
            180                 185                 190         


Gln Asp Gln Val Gln Gly Thr Val Thr Ile Arg Asn Ile Ser Ala Leu 
        195                 200                 205             


Ser Ser Gly Leu Tyr Gln Cys Val Ala Ser Asn Ala Ile Gly Thr Ser 
    210                 215                 220                 


Thr Cys Leu Leu Asp Leu Gln Val Ile Ser Pro Gln Pro Arg Asn Ile 
225                 230                 235                 240 


Gly Leu Ile Ala Gly Ser Glu Asn Leu Tyr Phe Gln Gly Asn Ser Gly 
                245                 250                 255     


Gly Gly Ser Gly Gly Gly Thr Gly Gly Gly Asp Leu Ala Pro Gln Met 
            260                 265                 270         


Leu Arg Glu Leu Gln Glu Thr Asn Ala Ala Leu Gln Asp Val Arg Glu 
        275                 280                 285             


Leu Leu Arg His Arg Val Lys Glu Ile Thr Phe Leu Lys Asn Thr Val 
    290                 295                 300                 


Met Glu Cys Asp Ala Cys Gly Leu Asp Arg Asn Leu Pro Pro Leu Ala 
305                 310                 315                 320 


Pro Leu Gly Pro His His His His His His 
                325                 330 


<210>  11
<211>  841
<212>  PRT
<213>  Artificial

<220>
<223>  VSIG8(1-263)-COMP-AlkPhos-Flag/His fusion

<400>  11

Met Arg Val Gly Gly Ala Phe His Leu Leu Leu Val Cys Leu Ser Pro 
1               5                   10                  15      


Ala Leu Leu Ser Ala Val Arg Ile Asn Gly Asp Gly Gln Glu Val Leu 
            20                  25                  30          


Tyr Leu Ala Glu Gly Asp Asn Val Arg Leu Gly Cys Pro Tyr Val Leu 
        35                  40                  45              


Asp Pro Glu Asp Tyr Gly Pro Asn Gly Leu Asp Ile Glu Trp Met Gln 
    50                  55                  60                  


Val Asn Ser Asp Pro Ala His His Arg Glu Asn Val Phe Leu Ser Tyr 
65                  70                  75                  80  


Gln Asp Lys Arg Ile Asn His Gly Ser Leu Pro His Leu Gln Gln Arg 
                85                  90                  95      


Val Arg Phe Ala Ala Ser Asp Pro Ser Gln Tyr Asp Ala Ser Ile Asn 
            100                 105                 110         


Leu Met Asn Leu Gln Val Ser Asp Thr Ala Thr Tyr Glu Cys Arg Val 
        115                 120                 125             


Lys Lys Thr Thr Met Ala Thr Arg Lys Val Ile Val Thr Val Gln Ala 
    130                 135                 140                 


Arg Pro Ala Val Pro Met Cys Trp Thr Glu Gly His Met Thr Tyr Gly 
145                 150                 155                 160 


Asn Asp Val Val Leu Lys Cys Tyr Ala Ser Gly Gly Ser Gln Pro Leu 
                165                 170                 175     


Ser Tyr Lys Trp Ala Lys Ile Ser Gly His His Tyr Pro Tyr Arg Ala 
            180                 185                 190         


Gly Ser Tyr Thr Ser Gln His Ser Tyr His Ser Glu Leu Ser Tyr Gln 
        195                 200                 205             


Glu Ser Phe His Ser Ser Ile Asn Gln Gly Leu Asn Asn Gly Asp Leu 
    210                 215                 220                 


Val Leu Lys Asp Ile Ser Arg Ala Asp Asp Gly Leu Tyr Gln Cys Thr 
225                 230                 235                 240 


Val Ala Asn Asn Val Gly Tyr Ser Val Cys Val Val Glu Val Lys Val 
                245                 250                 255     


Ser Asp Ser Arg Arg Ile Gly Gly Ser Glu Asn Leu Tyr Phe Gln Gly 
            260                 265                 270         


Asn Ser Gly Gly Gly Ser Gly Gly Gly Thr Gly Gly Gly Asp Leu Ala 
        275                 280                 285             


Pro Gln Met Leu Arg Glu Leu Gln Glu Thr Asn Ala Ala Leu Gln Asp 
    290                 295                 300                 


Val Arg Glu Leu Leu Arg His Arg Val Lys Glu Ile Thr Phe Leu Lys 
305                 310                 315                 320 


Asn Thr Val Met Glu Cys Asp Ala Cys Gly Leu Asp Arg Asn Leu Pro 
                325                 330                 335     


Pro Leu Ala Pro Leu Gly Pro Ile Ile Pro Val Glu Glu Glu Asn Pro 
            340                 345                 350         


Asp Phe Trp Asn Arg Glu Ala Ala Glu Ala Leu Gly Ala Ala Lys Lys 
        355                 360                 365             


Leu Gln Pro Ala Gln Thr Ala Ala Lys Asn Leu Ile Ile Phe Leu Gly 
    370                 375                 380                 


Asp Gly Met Gly Val Ser Thr Val Thr Ala Ala Arg Ile Leu Lys Gly 
385                 390                 395                 400 


Gln Lys Lys Asp Lys Leu Gly Pro Glu Ile Pro Leu Ala Met Asp Arg 
                405                 410                 415     


Phe Pro Tyr Val Ala Leu Ser Lys Thr Tyr Asn Val Asp Lys His Val 
            420                 425                 430         


Pro Asp Ser Gly Ala Thr Ala Thr Ala Tyr Leu Cys Gly Val Lys Gly 
        435                 440                 445             


Asn Phe Gln Thr Ile Gly Leu Ser Ala Ala Ala Arg Phe Asn Gln Cys 
    450                 455                 460                 


Asn Thr Thr Arg Gly Asn Glu Val Ile Ser Val Met Asn Arg Ala Lys 
465                 470                 475                 480 


Lys Ala Gly Lys Ser Val Gly Val Val Thr Thr Thr Arg Val Gln His 
                485                 490                 495     


Ala Ser Pro Ala Gly Thr Tyr Ala His Thr Val Asn Arg Asn Trp Tyr 
            500                 505                 510         


Ser Asp Ala Asp Val Pro Ala Ser Ala Arg Gln Glu Gly Cys Gln Asp 
        515                 520                 525             


Ile Ala Thr Gln Leu Ile Ser Asn Met Asp Ile Asp Val Ile Leu Gly 
    530                 535                 540                 


Gly Gly Arg Lys Tyr Met Phe Arg Met Gly Thr Pro Asp Pro Glu Tyr 
545                 550                 555                 560 


Pro Asp Asp Tyr Ser Gln Gly Gly Thr Arg Leu Asp Gly Lys Asn Leu 
                565                 570                 575     


Val Gln Glu Trp Leu Ala Lys Arg Gln Gly Ala Arg Tyr Val Trp Asn 
            580                 585                 590         


Arg Thr Glu Leu Met Gln Ala Ser Leu Asp Pro Ser Val Thr His Leu 
        595                 600                 605             


Met Gly Leu Phe Glu Pro Gly Asp Met Lys Tyr Glu Ile His Arg Asp 
    610                 615                 620                 


Ser Thr Leu Asp Pro Ser Leu Met Glu Met Thr Glu Ala Ala Leu Arg 
625                 630                 635                 640 


Leu Leu Ser Arg Asn Pro Arg Gly Phe Phe Leu Phe Val Glu Gly Gly 
                645                 650                 655     


Arg Ile Asp His Gly His His Glu Ser Arg Ala Tyr Arg Ala Leu Thr 
            660                 665                 670         


Glu Thr Ile Met Phe Asp Asp Ala Ile Glu Arg Ala Gly Gln Leu Thr 
        675                 680                 685             


Ser Glu Glu Asp Thr Leu Ser Leu Val Thr Ala Asp His Ser His Val 
    690                 695                 700                 


Phe Ser Phe Gly Gly Tyr Pro Leu Arg Gly Ser Ser Ile Phe Gly Leu 
705                 710                 715                 720 


Ala Pro Gly Lys Ala Arg Asp Arg Lys Ala Tyr Thr Val Leu Leu Tyr 
                725                 730                 735     


Gly Asn Gly Pro Gly Tyr Val Leu Lys Asp Gly Ala Arg Pro Asp Val 
            740                 745                 750         


Thr Glu Ser Glu Ser Gly Ser Pro Glu Tyr Arg Gln Gln Ser Ala Val 
        755                 760                 765             


Pro Leu Asp Glu Glu Thr His Ala Gly Glu Asp Val Ala Val Phe Ala 
    770                 775                 780                 


Arg Gly Pro Gln Ala His Leu Val His Gly Val Gln Glu Gln Thr Phe 
785                 790                 795                 800 


Ile Ala His Val Met Ala Phe Ala Ala Cys Leu Glu Pro Tyr Thr Ala 
                805                 810                 815     


Cys Asp Leu Ala Pro Pro Ala Gly Thr Thr Asp Asp Tyr Lys Asp Asp 
            820                 825                 830         


Asp Asp Lys His His His His His His 
        835                 840     


<210>  12
<211>  349
<212>  PRT
<213>  Artificial

<220>
<223>  VSIG8(1-263)-COMP-His fusion

<400>  12

Met Arg Val Gly Gly Ala Phe His Leu Leu Leu Val Cys Leu Ser Pro 
1               5                   10                  15      


Ala Leu Leu Ser Ala Val Arg Ile Asn Gly Asp Gly Gln Glu Val Leu 
            20                  25                  30          


Tyr Leu Ala Glu Gly Asp Asn Val Arg Leu Gly Cys Pro Tyr Val Leu 
        35                  40                  45              


Asp Pro Glu Asp Tyr Gly Pro Asn Gly Leu Asp Ile Glu Trp Met Gln 
    50                  55                  60                  


Val Asn Ser Asp Pro Ala His His Arg Glu Asn Val Phe Leu Ser Tyr 
65                  70                  75                  80  


Gln Asp Lys Arg Ile Asn His Gly Ser Leu Pro His Leu Gln Gln Arg 
                85                  90                  95      


Val Arg Phe Ala Ala Ser Asp Pro Ser Gln Tyr Asp Ala Ser Ile Asn 
            100                 105                 110         


Leu Met Asn Leu Gln Val Ser Asp Thr Ala Thr Tyr Glu Cys Arg Val 
        115                 120                 125             


Lys Lys Thr Thr Met Ala Thr Arg Lys Val Ile Val Thr Val Gln Ala 
    130                 135                 140                 


Arg Pro Ala Val Pro Met Cys Trp Thr Glu Gly His Met Thr Tyr Gly 
145                 150                 155                 160 


Asn Asp Val Val Leu Lys Cys Tyr Ala Ser Gly Gly Ser Gln Pro Leu 
                165                 170                 175     


Ser Tyr Lys Trp Ala Lys Ile Ser Gly His His Tyr Pro Tyr Arg Ala 
            180                 185                 190         


Gly Ser Tyr Thr Ser Gln His Ser Tyr His Ser Glu Leu Ser Tyr Gln 
        195                 200                 205             


Glu Ser Phe His Ser Ser Ile Asn Gln Gly Leu Asn Asn Gly Asp Leu 
    210                 215                 220                 


Val Leu Lys Asp Ile Ser Arg Ala Asp Asp Gly Leu Tyr Gln Cys Thr 
225                 230                 235                 240 


Val Ala Asn Asn Val Gly Tyr Ser Val Cys Val Val Glu Val Lys Val 
                245                 250                 255     


Ser Asp Ser Arg Arg Ile Gly Gly Ser Glu Asn Leu Tyr Phe Gln Gly 
            260                 265                 270         


Asn Ser Gly Gly Gly Ser Gly Gly Gly Thr Gly Gly Gly Asp Leu Ala 
        275                 280                 285             


Pro Gln Met Leu Arg Glu Leu Gln Glu Thr Asn Ala Ala Leu Gln Asp 
    290                 295                 300                 


Val Arg Glu Leu Leu Arg His Arg Val Lys Glu Ile Thr Phe Leu Lys 
305                 310                 315                 320 


Asn Thr Val Met Glu Cys Asp Ala Cys Gly Leu Asp Arg Asn Leu Pro 
                325                 330                 335     


Pro Leu Ala Pro Leu Gly Pro His His His His His His 
            340                 345                 


<210>  13
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  13

Asn Ser Gly Gly Gly Ser Gly Gly Gly Thr Gly 
1               5                   10      


<210>  14
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  14

Leu Asp Arg Asn Leu Pro Pro Leu Ala Pro Leu Gly Pro 
1               5                   10              


