                         SEQUENCE LISTING

<110>  Beijing Percans Oncology Co., Ltd.
       Chen, Yiyou
       Guo, Dagang
 
<120>  Cytokine Fusion Proteins

<130>  BEPE-004/02WO 327425-2016

<150>  PCT/CN2018/119071
<151>  2018-12-04

<150>  PCT/CN2018/073940
<151>  2018-01-24

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  153
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
            20                  25                  30          


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
        35                  40                  45              


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
    50                  55                  60                  


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
65                  70                  75                  80  


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
                85                  90                  95      


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
            100                 105                 110         


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
        115                 120                 125             


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
    130                 135                 140                 


Cys Gln Ser Ile Ile Ser Thr Leu Thr 
145                 150             


<210>  2
<211>  153
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
            20                  25                  30          


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
        35                  40                  45              


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
    50                  55                  60                  


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
65                  70                  75                  80  


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
                85                  90                  95      


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
            100                 105                 110         


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
        115                 120                 125             


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
    130                 135                 140                 


Ser Gln Ser Ile Ile Ser Thr Leu Thr 
145                 150             


<210>  3
<211>  233
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Thr Glu Ser Met Ile Arg Asp Val Glu Leu Ala Glu Glu Ala 
1               5                   10                  15      


Leu Pro Lys Lys Thr Gly Gly Pro Gln Gly Ser Arg Arg Cys Leu Phe 
            20                  25                  30          


Leu Ser Leu Phe Ser Phe Leu Ile Val Ala Gly Ala Thr Thr Leu Phe 
        35                  40                  45              


Cys Leu Leu His Phe Gly Val Ile Gly Pro Gln Arg Glu Glu Phe Pro 
    50                  55                  60                  


Arg Asp Leu Ser Leu Ile Ser Pro Leu Ala Gln Ala Val Arg Ser Ser 
65                  70                  75                  80  


Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro 
                85                  90                  95      


Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu 
            100                 105                 110         


Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser 
        115                 120                 125             


Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly 
    130                 135                 140                 


Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala 
145                 150                 155                 160 


Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro 
                165                 170                 175     


Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu 
            180                 185                 190         


Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu 
        195                 200                 205             


Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly 
    210                 215                 220                 


Gln Val Tyr Phe Gly Ile Ile Ala Leu 
225                 230             


<210>  4
<211>  157
<212>  PRT
<213>  Homo sapiens

<400>  4

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  5
<211>  157
<212>  PRT
<213>  Homo sapiens

<400>  5

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Glu Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  6
<211>  157
<212>  PRT
<213>  Homo sapiens

<400>  6

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ala Val Thr Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  7
<211>  157
<212>  PRT
<213>  Homo sapiens

<400>  7

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Glu Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ala Val Thr Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  8
<211>  219
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val Leu Leu 
1               5                   10                  15      


Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro Asp Pro 
            20                  25                  30          


Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala Val 
        35                  40                  45              


Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys 
    50                  55                  60                  


Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr Ser 
65                  70                  75                  80  


Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys 
                85                  90                  95      


Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala 
            100                 105                 110         


Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile Tyr 
        115                 120                 125             


Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala Lys 
    130                 135                 140                 


Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met Leu 
145                 150                 155                 160 


Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu Thr 
                165                 170                 175     


Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys 
            180                 185                 190         


Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val Thr 
        195                 200                 205             


Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
    210                 215                 


<210>  9
<211>  328
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      


Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser 
                325             


<210>  10
<211>  144
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Trp Leu Gln Ser Leu Leu Leu Leu Gly Thr Val Ala Cys Ser Ile 
1               5                   10                  15      


Ser Ala Pro Ala Arg Ser Pro Ser Pro Ser Thr Gln Pro Trp Glu His 
            20                  25                  30          


Val Asn Ala Ile Gln Glu Ala Arg Arg Leu Leu Asn Leu Ser Arg Asp 
        35                  40                  45              


Thr Ala Ala Glu Met Asn Glu Thr Val Glu Val Ile Ser Glu Met Phe 
    50                  55                  60                  


Asp Leu Gln Glu Pro Thr Cys Leu Gln Thr Arg Leu Glu Leu Tyr Lys 
65                  70                  75                  80  


Gln Gly Leu Arg Gly Ser Leu Thr Lys Leu Lys Gly Pro Leu Thr Met 
                85                  90                  95      


Met Ala Ser His Tyr Lys Gln His Cys Pro Pro Thr Pro Glu Thr Ser 
            100                 105                 110         


Cys Ala Thr Gln Ile Ile Thr Phe Glu Ser Phe Lys Glu Asn Leu Lys 
        115                 120                 125             


Asp Phe Leu Leu Val Ile Pro Phe Asp Cys Trp Glu Pro Val Gln Glu 
    130                 135                 140                 


<210>  11
<211>  177
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Phe His Val Ser Phe Arg Tyr Ile Phe Gly Leu Pro Pro Leu Ile 
1               5                   10                  15      


Leu Val Leu Leu Pro Val Ala Ser Ser Asp Cys Asp Ile Glu Gly Lys 
            20                  25                  30          


Asp Gly Lys Gln Tyr Glu Ser Val Leu Met Val Ser Ile Asp Gln Leu 
        35                  40                  45              


Leu Asp Ser Met Lys Glu Ile Gly Ser Asn Cys Leu Asn Asn Glu Phe 
    50                  55                  60                  


Asn Phe Phe Lys Arg His Ile Cys Asp Ala Asn Lys Glu Gly Met Phe 
65                  70                  75                  80  


Leu Phe Arg Ala Ala Arg Lys Leu Arg Gln Phe Leu Lys Met Asn Ser 
                85                  90                  95      


Thr Gly Asp Phe Asp Leu His Leu Leu Lys Val Ser Glu Gly Thr Thr 
            100                 105                 110         


Ile Leu Leu Asn Cys Thr Gly Gln Val Lys Gly Arg Lys Pro Ala Ala 
        115                 120                 125             


Leu Gly Glu Ala Gln Pro Thr Lys Ser Leu Glu Glu Asn Lys Ser Leu 
    130                 135                 140                 


Lys Glu Gln Lys Lys Leu Asn Asp Leu Cys Phe Leu Lys Arg Leu Leu 
145                 150                 155                 160 


Gln Glu Ile Lys Thr Cys Trp Asn Lys Ile Leu Met Gly Thr Lys Glu 
                165                 170                 175     


His 
    


<210>  12
<211>  189
<212>  PRT
<213>  Homo sapiens

<400>  12

Met Leu Gly Ser Arg Ala Val Met Leu Leu Leu Leu Leu Pro Trp Thr 
1               5                   10                  15      


Ala Gln Gly Arg Ala Val Pro Gly Gly Ser Ser Pro Ala Trp Thr Gln 
            20                  25                  30          


Cys Gln Gln Leu Ser Gln Lys Leu Cys Thr Leu Ala Trp Ser Ala His 
        35                  40                  45              


Pro Leu Val Gly His Met Asp Leu Arg Glu Glu Gly Asp Glu Glu Thr 
    50                  55                  60                  


Thr Asn Asp Val Pro His Ile Gln Cys Gly Asp Gly Cys Asp Pro Gln 
65                  70                  75                  80  


Gly Leu Arg Asp Asn Ser Gln Phe Cys Leu Gln Arg Ile His Gln Gly 
                85                  90                  95      


Leu Ile Phe Tyr Glu Lys Leu Leu Gly Ser Asp Ile Phe Thr Gly Glu 
            100                 105                 110         


Pro Ser Leu Leu Pro Asp Ser Pro Val Gly Gln Leu His Ala Ser Leu 
        115                 120                 125             


Leu Gly Leu Ser Gln Leu Leu Gln Pro Glu Gly His His Trp Glu Thr 
    130                 135                 140                 


Gln Gln Ile Pro Ser Leu Ser Pro Ser Gln Pro Trp Gln Arg Leu Leu 
145                 150                 155                 160 


Leu Arg Phe Lys Ile Leu Arg Ser Leu Gln Ala Phe Val Ala Val Ala 
                165                 170                 175     


Ala Arg Val Phe Ala His Gly Ala Ala Thr Leu Ser Pro 
            180                 185                 


<210>  13
<211>  243
<212>  PRT
<213>  Homo sapiens

<400>  13

Met Gly Gln Thr Ala Gly Asp Leu Gly Trp Arg Leu Ser Leu Leu Leu 
1               5                   10                  15      


Leu Pro Leu Leu Leu Val Gln Ala Gly Val Trp Gly Phe Pro Arg Pro 
            20                  25                  30          


Pro Gly Arg Pro Gln Leu Ser Leu Gln Glu Leu Arg Arg Glu Phe Thr 
        35                  40                  45              


Val Ser Leu His Leu Ala Arg Lys Leu Leu Ser Glu Val Arg Gly Gln 
    50                  55                  60                  


Ala His Arg Phe Ala Glu Ser His Leu Pro Gly Val Asn Leu Tyr Leu 
65                  70                  75                  80  


Leu Pro Leu Gly Glu Gln Leu Pro Asp Val Ser Leu Thr Phe Gln Ala 
                85                  90                  95      


Trp Arg Arg Leu Ser Asp Pro Glu Arg Leu Cys Phe Ile Ser Thr Thr 
            100                 105                 110         


Leu Gln Pro Phe His Ala Leu Leu Gly Gly Leu Gly Thr Gln Gly Arg 
        115                 120                 125             


Trp Thr Asn Met Glu Arg Met Gln Leu Trp Ala Met Arg Leu Asp Leu 
    130                 135                 140                 


Arg Asp Leu Gln Arg His Leu Arg Phe Gln Val Leu Ala Ala Gly Phe 
145                 150                 155                 160 


Asn Leu Pro Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu 
                165                 170                 175     


Arg Lys Gly Leu Leu Pro Gly Ala Leu Gly Ser Ala Leu Gln Gly Pro 
            180                 185                 190         


Ala Gln Val Ser Trp Pro Gln Leu Leu Ser Thr Tyr Arg Leu Leu His 
        195                 200                 205             


Ser Leu Glu Leu Val Leu Ser Arg Ala Val Arg Glu Leu Leu Leu Leu 
    210                 215                 220                 


Ser Lys Ala Gly His Ser Val Trp Pro Leu Gly Phe Pro Thr Leu Ser 
225                 230                 235                 240 


Pro Gln Pro 
            


<210>  14
<211>  229
<212>  PRT
<213>  Homo sapiens

<400>  14

Met Thr Pro Gln Leu Leu Leu Ala Leu Val Leu Trp Ala Ser Cys Pro 
1               5                   10                  15      


Pro Cys Ser Gly Arg Lys Gly Pro Pro Ala Ala Leu Thr Leu Pro Arg 
            20                  25                  30          


Val Gln Cys Arg Ala Ser Arg Tyr Pro Ile Ala Val Asp Cys Ser Trp 
        35                  40                  45              


Thr Leu Pro Pro Ala Pro Asn Ser Thr Ser Pro Val Ser Phe Ile Ala 
    50                  55                  60                  


Thr Tyr Arg Leu Gly Met Ala Ala Arg Gly His Ser Trp Pro Cys Leu 
65                  70                  75                  80  


Gln Gln Thr Pro Thr Ser Thr Ser Cys Thr Ile Thr Asp Val Gln Leu 
                85                  90                  95      


Phe Ser Met Ala Pro Tyr Val Leu Asn Val Thr Ala Val His Pro Trp 
            100                 105                 110         


Gly Ser Ser Ser Ser Phe Val Pro Phe Ile Thr Glu His Ile Ile Lys 
        115                 120                 125             


Pro Asp Pro Pro Glu Gly Val Arg Leu Ser Pro Leu Ala Glu Arg Gln 
    130                 135                 140                 


Leu Gln Val Gln Trp Glu Pro Pro Gly Ser Trp Pro Phe Pro Glu Ile 
145                 150                 155                 160 


Phe Ser Leu Lys Tyr Trp Ile Arg Tyr Lys Arg Gln Gly Ala Ala Arg 
                165                 170                 175     


Phe His Arg Val Gly Pro Ile Glu Ala Thr Ser Phe Ile Leu Arg Ala 
            180                 185                 190         


Val Arg Pro Arg Ala Arg Tyr Tyr Val Gln Val Ala Ala Gln Asp Leu 
        195                 200                 205             


Thr Asp Tyr Gly Glu Leu Ser Asp Trp Ser Leu Pro Ala Thr Ala Thr 
    210                 215                 220                 


Met Ser Leu Gly Lys 
225                 


<210>  15
<211>  187
<212>  PRT
<213>  Homo sapiens

<400>  15

Met Thr Asn Lys Cys Leu Leu Gln Ile Ala Leu Leu Leu Cys Phe Ser 
1               5                   10                  15      


Thr Thr Ala Leu Ser Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg 
            20                  25                  30          


Ser Ser Asn Phe Gln Cys Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg 
        35                  40                  45              


Leu Glu Tyr Cys Leu Lys Asp Arg Met Asn Phe Asp Ile Pro Glu Glu 
    50                  55                  60                  


Ile Lys Gln Leu Gln Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile 
65                  70                  75                  80  


Tyr Glu Met Leu Gln Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser 
                85                  90                  95      


Ser Thr Gly Trp Asn Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val 
            100                 105                 110         


Tyr His Gln Ile Asn His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu 
        115                 120                 125             


Lys Glu Asp Phe Thr Arg Gly Lys Leu Met Ser Ser Leu His Leu Lys 
    130                 135                 140                 


Arg Tyr Tyr Gly Arg Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser 
145                 150                 155                 160 


His Cys Ala Trp Thr Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr 
                165                 170                 175     


Phe Ile Asn Arg Leu Thr Gly Tyr Leu Arg Asn 
            180                 185         


<210>  16
<211>  189
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Ala Ser Pro Phe Ala Leu Leu Met Val Leu Val Val Leu Ser Cys 
1               5                   10                  15      


Lys Ser Ser Cys Ser Leu Gly Cys Asp Leu Pro Glu Thr His Ser Leu 
            20                  25                  30          


Asp Asn Arg Arg Thr Leu Met Leu Leu Ala Gln Met Ser Arg Ile Ser 
        35                  40                  45              


Pro Ser Ser Cys Leu Met Asp Arg His Asp Phe Gly Phe Pro Gln Glu 
    50                  55                  60                  


Glu Phe Asp Gly Asn Gln Phe Gln Lys Ala Pro Ala Ile Ser Val Leu 
65                  70                  75                  80  


His Glu Leu Ile Gln Gln Ile Phe Asn Leu Phe Thr Thr Lys Asp Ser 
                85                  90                  95      


Ser Ala Ala Trp Asp Glu Asp Leu Leu Asp Lys Phe Cys Thr Glu Leu 
            100                 105                 110         


Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Met Gln Glu Glu Arg 
        115                 120                 125             


Val Gly Glu Thr Pro Leu Met Asn Ala Asp Ser Ile Leu Ala Val Lys 
    130                 135                 140                 


Lys Tyr Phe Arg Arg Ile Thr Leu Tyr Leu Thr Glu Lys Lys Tyr Ser 
145                 150                 155                 160 


Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Leu Ser 
                165                 170                 175     


Leu Ser Thr Asn Leu Gln Glu Arg Leu Arg Arg Lys Glu 
            180                 185                 


<210>  17
<211>  188
<212>  PRT
<213>  Homo sapiens

<400>  17

Met Ala Leu Thr Phe Ala Leu Leu Val Ala Leu Leu Val Leu Ser Cys 
1               5                   10                  15      


Lys Ser Ser Cys Ser Val Gly Cys Asp Leu Pro Gln Thr His Ser Leu 
            20                  25                  30          


Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln Met Arg Lys Ile Ser 
        35                  40                  45              


Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe Gly Phe Pro Gln Glu 
    50                  55                  60                  


Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu His 
65                  70                  75                  80  


Glu Met Ile Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp Ser Ser 
                85                  90                  95      


Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe Tyr Thr Glu Leu Tyr 
            100                 105                 110         


Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly Val 
        115                 120                 125             


Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg Lys 
    130                 135                 140                 


Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu Lys Lys Tyr Ser Pro 
145                 150                 155                 160 


Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser Leu 
                165                 170                 175     


Ser Thr Asn Leu Gln Glu Ser Leu Arg Ser Lys Glu 
            180                 185             


<210>  18
<211>  166
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Lys Tyr Thr Ser Tyr Ile Leu Ala Phe Gln Leu Cys Ile Val Leu 
1               5                   10                  15      


Gly Ser Leu Gly Cys Tyr Cys Gln Asp Pro Tyr Val Lys Glu Ala Glu 
            20                  25                  30          


Asn Leu Lys Lys Tyr Phe Asn Ala Gly His Ser Asp Val Ala Asp Asn 
        35                  40                  45              


Gly Thr Leu Phe Leu Gly Ile Leu Lys Asn Trp Lys Glu Glu Ser Asp 
    50                  55                  60                  


Arg Lys Ile Met Gln Ser Gln Ile Val Ser Phe Tyr Phe Lys Leu Phe 
65                  70                  75                  80  


Lys Asn Phe Lys Asp Asp Gln Ser Ile Gln Lys Ser Val Glu Thr Ile 
                85                  90                  95      


Lys Glu Asp Met Asn Val Lys Phe Phe Asn Ser Asn Lys Lys Lys Arg 
            100                 105                 110         


Asp Asp Phe Glu Lys Leu Thr Asn Tyr Ser Val Thr Asp Leu Asn Val 
        115                 120                 125             


Gln Arg Lys Ala Ile His Glu Leu Ile Gln Val Met Ala Glu Leu Ser 
    130                 135                 140                 


Pro Ala Ala Lys Thr Gly Lys Arg Lys Arg Ser Gln Met Leu Phe Arg 
145                 150                 155                 160 


Gly Arg Arg Ala Ser Gln 
                165     


<210>  19
<211>  305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IL2(C125S)-(GGGGS)3-TNFalpha (S86T) fusion 
       construct

<400>  19

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro 
145                 150                 155                 160 


Val Ala His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp 
                165                 170                 175     


Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg 
            180                 185                 190         


Asp Asn Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser 
        195                 200                 205             


Gln Val Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu 
    210                 215                 220                 


Thr His Thr Ile Ser Arg Ile Ala Val Thr Tyr Gln Thr Lys Val Asn 
225                 230                 235                 240 


Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly 
                245                 250                 255     


Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe 
            260                 265                 270         


Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp 
        275                 280                 285             


Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala 
    290                 295                 300                 


Leu 
305 


<210>  20
<211>  300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IL2(C125S)-(GGGGS)2-TNFalpha (S86T) fusion 
       construct

<400>  20

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val 
    130                 135                 140                 


Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val 
145                 150                 155                 160 


Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala 
                165                 170                 175     


Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val 
            180                 185                 190         


Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys 
        195                 200                 205             


Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser 
    210                 215                 220                 


Arg Ile Ala Val Thr Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile 
225                 230                 235                 240 


Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro 
                245                 250                 255     


Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly 
            260                 265                 270         


Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala 
        275                 280                 285             


Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
    290                 295                 300 


<210>  21
<211>  300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IL2(C125S)-(GGGGS)2-TNFalpha (R32W) fusion 
       construct

<400>  21

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Val 
    130                 135                 140                 


Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val 
145                 150                 155                 160 


Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Trp Ala 
                165                 170                 175     


Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val 
            180                 185                 190         


Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys 
        195                 200                 205             


Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser 
    210                 215                 220                 


Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile 
225                 230                 235                 240 


Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro 
                245                 250                 255     


Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly 
            260                 265                 270         


Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala 
        275                 280                 285             


Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
    290                 295                 300 


<210>  22
<211>  295
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IL2(C125S)-PAPAP-TNFalpha (S86T) fusion construct

<400>  22

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Pro Ala Pro Ala Pro Val Arg Ser Ser Ser Arg 
    130                 135                 140                 


Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro Gln Ala 
145                 150                 155                 160 


Glu Gly Gln Leu Gln Trp Leu Asn Arg Trp Ala Asn Ala Leu Leu Ala 
                165                 170                 175     


Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser Glu Gly 
            180                 185                 190         


Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
        195                 200                 205             


Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala Val Thr 
    210                 215                 220                 


Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln 
225                 230                 235                 240 


Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile 
                245                 250                 255     


Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala 
            260                 265                 270         


Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val 
        275                 280                 285             


Tyr Phe Gly Ile Ile Ala Leu 
    290                 295 


<210>  23
<211>  295
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IL2(C125S)-PAPAP-TNFalpha (R32W) fusion construct

<400>  23

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Pro Ala Pro Ala Pro Val Arg Ser Ser Ser Arg 
    130                 135                 140                 


Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro Gln Ala 
145                 150                 155                 160 


Glu Gly Gln Leu Gln Trp Leu Asn Arg Trp Ala Asn Ala Leu Leu Ala 
                165                 170                 175     


Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser Glu Gly 
            180                 185                 190         


Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro 
        195                 200                 205             


Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala Val Ser 
    210                 215                 220                 


Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln 
225                 230                 235                 240 


Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile 
                245                 250                 255     


Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala 
            260                 265                 270         


Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val 
        275                 280                 285             


Tyr Phe Gly Ile Ile Ala Leu 
    290                 295 


<210>  24
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IL2(C125S)-PAEAAAKEAAAKA-TNFalpha (S86T) fusion 
       construct

<400>  24

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
    130                 135                 140                 


Ala Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His 
145                 150                 155                 160 


Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg 
                165                 170                 175     


Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln 
            180                 185                 190         


Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu 
        195                 200                 205             


Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr 
    210                 215                 220                 


Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser 
225                 230                 235                 240 


Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala 
                245                 250                 255     


Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu 
            260                 265                 270         


Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp 
        275                 280                 285             


Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
    290                 295                 300         


<210>  25
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IL2(C125S)-PAEAAAKEAAAKA-TNFalpha (R32W) fusion 
       construct

<400>  25

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
    130                 135                 140                 


Ala Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His 
145                 150                 155                 160 


Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg 
                165                 170                 175     


Trp Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln 
            180                 185                 190         


Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu 
        195                 200                 205             


Phe Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr 
    210                 215                 220                 


Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser 
225                 230                 235                 240 


Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala 
                245                 250                 255     


Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu 
            260                 265                 270         


Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp 
        275                 280                 285             


Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
    290                 295                 300         


<210>  26
<211>  353
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - IFNbeta-GGGGS2-TNFalpha fusion construct

<400>  26

Met Thr Asn Lys Cys Leu Leu Gln Ile Ala Leu Leu Leu Cys Phe Ser 
1               5                   10                  15      


Thr Thr Ala Leu Ser Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg 
            20                  25                  30          


Ser Ser Asn Phe Gln Cys Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg 
        35                  40                  45              


Leu Glu Tyr Cys Leu Lys Asp Arg Met Asn Phe Asp Ile Pro Glu Glu 
    50                  55                  60                  


Ile Lys Gln Leu Gln Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile 
65                  70                  75                  80  


Tyr Glu Met Leu Gln Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser 
                85                  90                  95      


Ser Thr Gly Trp Asn Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val 
            100                 105                 110         


Tyr His Gln Ile Asn His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu 
        115                 120                 125             


Lys Glu Asp Phe Thr Arg Gly Lys Leu Met Ser Ser Leu His Leu Lys 
    130                 135                 140                 


Arg Tyr Tyr Gly Arg Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser 
145                 150                 155                 160 


His Cys Ala Trp Thr Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr 
                165                 170                 175     


Phe Ile Asn Arg Leu Thr Gly Tyr Leu Arg Asn Gly Gly Gly Gly Ser 
            180                 185                 190         


Gly Gly Gly Gly Ser Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys 
        195                 200                 205             


Pro Val Ala His Val Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln 
    210                 215                 220                 


Trp Leu Asn Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu 
225                 230                 235                 240 


Arg Asp Asn Gln Leu Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr 
                245                 250                 255     


Ser Gln Val Leu Phe Lys Gly Gln Gly Cys Pro Ser Thr His Leu Leu 
            260                 265                 270         


Thr His Thr Ile Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn 
        275                 280                 285             


Leu Leu Ser Ala Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly 
    290                 295                 300                 


Ala Glu Ala Lys Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe 
305                 310                 315                 320 


Gln Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp 
                325                 330                 335     


Tyr Leu Asp Phe Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala 
            340                 345                 350         


Leu 
    


<210>  27
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  27

Gly Ser Gly Ser 
1               


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  28

Gly Gly Ser Gly 
1               


<210>  29
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  29

Gly Gly Gly Ser 
1               


<210>  30
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  30

Gly Gly Gly Gly Ser 
1               5   


<210>  31
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  31

Gly Asn Gly Asn 
1               


<210>  32
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  32

Gly Gly Asn Gly 
1               


<210>  33
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  33

Gly Gly Gly Asn 
1               


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  34

Gly Gly Gly Gly Asn 
1               5   


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  35

Ala Glu Ala Ala Ala Lys Ala 
1               5           


<210>  36
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  36

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
1               5                   10          


<210>  37
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  37

Ala Pro Ala Pro Lys Pro 
1               5       


<210>  38
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  38

Ala Pro Ala Pro Lys Pro Glu Pro Ala Pro Lys Pro 
1               5                   10          


<210>  39
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  39

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  40
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  40

Asp Gly Gly Gly Ser 
1               5   


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  41

Thr Gly Glu Lys Pro 
1               5   


<210>  42
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  42

Gly Gly Arg Arg 
1               


<210>  43
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  43

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  44
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  44

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  45
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  45

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  46
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  46

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  47
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  47

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  48
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  48

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  49
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  49

Pro Ala Pro Ala Pro 
1               5   


<210>  50
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized peptide linker

<400>  50

Pro Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
1               5                   10              


<210>  51
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kozak consensus sequence

<400>  51
rccrccatgg                                                              10


<210>  52
<211>  1065
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - mRNA for IFNbeta-GGGGS2-TNFalpha fusion construct

<400>  52
atgaccaaca agtgtctcct ccaaattgct ctcctgttgt gcttctccac tacagctctt       60

tccatgagct acaacttgct tggattccta caaagaagca gcaattttca gtgtcagaag      120

ctcctgtggc aattgaatgg gaggcttgaa tactgcctca aggacaggat gaactttgac      180

atccctgagg agattaagca gctgcagcag ttccagaagg aggacgccgc attgaccatc      240

tatgagatgc tccagaacat ctttgctatt ttcagacaag attcatctag cactggctgg      300

aatgagacta ttgttgagaa cctcctggct aatgtctatc atcagataaa ccatctgaag      360

acagtcctgg aagaaaaact ggagaaagaa gatttcacca ggggaaaact catgagcagt      420

ctgcacctga aaagatatta tgggaggatt ctgcattacc tgaaggccaa ggagtacagt      480

cactgtgcct ggaccatagt cagagtggaa atcctaagga acttttactt cattaacaga      540

cttacaggtt acctccgaaa cggtggcggt ggctctggag ggggagggtc cgtcagatca      600

tcttctcgaa ccccgagtga caagcctgta gcccatgttg tagcaaaccc tcaagctgag      660

gggcagctcc agtggctgaa ccgccgggcc aatgccctcc tggccaatgg cgtggagctg      720

agagataacc agctggtggt gccatcagag ggcctgtacc tcatctactc ccaggtcctc      780

ttcaagggcc aaggctgccc ctccacccat gtgctcctca cccacaccat cagccgcatc      840

gccgtctcct accagaccaa ggtcaacctc ctctctgcca tcaagagccc ctgccagagg      900

gagaccccag agggggctga ggccaagccc tggtatgagc ccatctatct gggaggggtc      960

ttccagctgg agaagggtga ccgactcagc gctgagatca atcggcccga ctatctcgac     1020

tttgccgagt ctgggcaggt ctactttggg atcattgccc tgtga                     1065


