                         SEQUENCE LISTING

<110>  University of Florida Research Foundation, Inc.
       Curtiss, Roy   III
       Swain, Banikalyan
 
<120>  ICHTHYOPHTHIRIUS MULTIFILIIS VACCINE SYSTEM

<130>  222110-2640; UF# 17144

<150>  US 62/621,567
<151>  2018-01-23

<150>  US 62/621,567
<151>  2018-01-24

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1329
<212>  DNA
<213>  Ichthyophthirius multifiliis


<220>
<221>  misc_feature
<222>  (1)..(1329)
<223>  IchIAG48 (GenBank: AF140273.1)

<400>  1
atgaaatata atattttatt aattttaatt atttctttat ttattaatga attaagagct       60

gttccatgtc ctgatggtac ttagactcaa gctggattga ctgatgtagg tgctgctgat      120

cttggtactt gtgttaattg cagacctaat ttttactata atggtggtgc tgcttaagga      180

gaagctaatg gtaattaacc tttcgcagca aataatgctg ctagaggtat atgtgtacca      240

tgccaaataa acagagtagg ctctgttacc aatgcaggtg acttagctac tttagccaca      300

taatgcagta cttaatgtcc tactggcact gcacttgatg atggagtgac agatgttttt      360

gatagatcag ccgcataatg tgttaaatgc aaacctaact tttactataa tggtggttct      420

ccttaaggtg aagctcctgg cgtttaagtt tttgctgctg gtgctgccgc tgcaggtgtt      480

gctgccgtta ctagttaatg tgtaccttgc caactaaaca aaaacgattc tcctgccact      540

gcaggtgcct aagctaattt agccacataa tgtagcaatt aatgtcctac tggcactgta      600

cttgatgatg gagtgacact tgtttttaat acatcagcca cattatgtgt taaatgcaga      660

cctaactttt actataatgg tggttctcct taaggtgaag ctcctggcgt ttaagttttt      720

gctgctggtg ctgccgctgc aggtgttgct gccgttacta gttaatgtgt accttgccaa      780

ataaacaaaa acgattctcc tgccactgca ggtgcctaag ctaatttagc cacataatgc      840

agtacttaat gtccaactgg cactgcaatt caagacggag tgacacttgt ttttagtaat      900

tcatccacat aatgttctta atgcattgct aattactttt ttaatggtaa tttcgaagca      960

ggtaaaagtt aatgtttaaa gtgtccagta agtaaaacta ctccagcaca tgctccaggt     1020

aatactgcta cttaagccac ataatgtttg accacatgtc ctgctggtac agtacttgat     1080

gatggaacat caactaattt tgtagcttcc gcaactgaat gtactaaatg ttctgctggc     1140

ttttttgcat caaaaacaac tggttttaca gcaggtactg atacatgtac tgaatgtact     1200

aaaaaattaa cttctggtgc cacagctaaa gtatatgctg aagctactca aaaagtataa     1260

tgcgcctcca ctactttcgc taaattttta tcgatttcct tattatttat ttctttctat     1320

ttattgtga                                                             1329


<210>  2
<211>  442
<212>  PRT
<213>  Ichthyophthirius multifiliis


<220>
<221>  MISC_FEATURE
<222>  (1)..(442)
<223>  Ich IAG48 (GenBank: AF140273.1) protein sequence

<400>  2

Met Lys Tyr Asn Ile Leu Leu Ile Leu Ile Ile Ser Leu Phe Ile Asn 
1               5                   10                  15      


Glu Leu Arg Ala Val Pro Cys Pro Asp Gly Thr Gln Thr Gln Ala Gly 
            20                  25                  30          


Leu Thr Asp Val Gly Ala Ala Asp Leu Gly Thr Cys Val Asn Cys Arg 
        35                  40                  45              


Pro Asn Phe Tyr Tyr Asn Gly Gly Ala Ala Gln Gly Glu Ala Asn Gly 
    50                  55                  60                  


Asn Gln Pro Phe Ala Ala Asn Asn Ala Ala Arg Gly Ile Cys Val Pro 
65                  70                  75                  80  


Cys Gln Ile Asn Arg Val Gly Ser Val Thr Asn Ala Gly Asp Leu Ala 
                85                  90                  95      


Thr Leu Ala Thr Gln Cys Ser Thr Gln Cys Pro Thr Gly Thr Ala Leu 
            100                 105                 110         


Asp Asp Gly Val Thr Asp Val Phe Asp Arg Ser Ala Ala Gln Cys Val 
        115                 120                 125             


Lys Cys Lys Pro Asn Phe Tyr Tyr Asn Gly Gly Ser Pro Gln Gly Glu 
    130                 135                 140                 


Ala Pro Gly Val Gln Val Phe Ala Ala Gly Ala Ala Ala Ala Gly Val 
145                 150                 155                 160 


Ala Ala Val Thr Ser Gln Cys Val Pro Cys Gln Leu Asn Lys Asn Asp 
                165                 170                 175     


Ser Pro Ala Thr Ala Gly Ala Gln Ala Asn Leu Ala Thr Gln Cys Ser 
            180                 185                 190         


Asn Gln Cys Pro Thr Gly Thr Val Leu Asp Asp Gly Val Thr Leu Val 
        195                 200                 205             


Phe Asn Thr Ser Ala Thr Leu Cys Val Lys Cys Arg Pro Asn Phe Tyr 
    210                 215                 220                 


Tyr Asn Gly Gly Ser Pro Gln Gly Glu Ala Pro Gly Val Gln Val Phe 
225                 230                 235                 240 


Ala Ala Gly Ala Ala Ala Ala Gly Val Ala Ala Val Thr Ser Gln Cys 
                245                 250                 255     


Val Pro Cys Gln Ile Asn Lys Asn Asp Ser Pro Ala Thr Ala Gly Ala 
            260                 265                 270         


Gln Ala Asn Leu Ala Thr Gln Cys Ser Thr Gln Cys Pro Thr Gly Thr 
        275                 280                 285             


Ala Ile Gln Asp Gly Val Thr Leu Val Phe Ser Asn Ser Ser Thr Gln 
    290                 295                 300                 


Cys Ser Gln Cys Ile Ala Asn Tyr Phe Phe Asn Gly Asn Phe Glu Ala 
305                 310                 315                 320 


Gly Lys Ser Gln Cys Leu Lys Cys Pro Val Ser Lys Thr Thr Pro Ala 
                325                 330                 335     


His Ala Pro Gly Asn Thr Ala Thr Gln Ala Thr Gln Cys Leu Thr Thr 
            340                 345                 350         


Cys Pro Ala Gly Thr Val Leu Asp Asp Gly Thr Ser Thr Asn Phe Val 
        355                 360                 365             


Ala Ser Ala Thr Glu Cys Thr Lys Cys Ser Ala Gly Phe Phe Ala Ser 
    370                 375                 380                 


Lys Thr Thr Gly Phe Thr Ala Gly Thr Asp Thr Cys Thr Glu Cys Thr 
385                 390                 395                 400 


Lys Lys Leu Thr Ser Gly Ala Thr Ala Lys Val Tyr Ala Glu Ala Thr 
                405                 410                 415     


Gln Lys Val Gln Cys Ala Ser Thr Thr Phe Ala Lys Phe Leu Ser Ile 
            420                 425                 430         


Ser Leu Leu Phe Ile Ser Phe Tyr Leu Leu 
        435                 440         


<210>  3
<211>  1329
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ich IAG48 (TAA and TAG codons changed to CAG (* to Q))

<400>  3
atgaaatata atattttatt aattttaatt atttctttat ttattaatga attaagagct       60

gttccatgtc ctgatggtac tcagactcaa gctggattga ctgatgtagg tgctgctgat      120

cttggtactt gtgttaattg cagacctaat ttttactata atggtggtgc tgctcaggga      180

gaagctaatg gtaatcagcc tttcgcagca aataatgctg ctagaggtat atgtgtacca      240

tgccaaataa acagagtagg ctctgttacc aatgcaggtg acttagctac tttagccaca      300

cagtgcagta ctcagtgtcc tactggcact gcacttgatg atggagtgac agatgttttt      360

gatagatcag ccgcacagtg tgttaaatgc aaacctaact tttactataa tggtggttct      420

cctcagggtg aagctcctgg cgttcaggtt tttgctgctg gtgctgccgc tgcaggtgtt      480

gctgccgtta ctagtcagtg tgtaccttgc caactaaaca aaaacgattc tcctgccact      540

gcaggtgccc aggctaattt agccacacag tgtagcaatc agtgtcctac tggcactgta      600

cttgatgatg gagtgacact tgtttttaat acatcagcca cattatgtgt taaatgcaga      660

cctaactttt actataatgg tggttctcct cagggtgaag ctcctggcgt tcaggttttt      720

gctgctggtg ctgccgctgc aggtgttgct gccgttacta gtcagtgtgt accttgccaa      780

ataaacaaaa acgattctcc tgccactgca ggtgcccagg ctaatttagc cacacagtgc      840

agtactcagt gtccaactgg cactgcaatt caagacggag tgacacttgt ttttagtaat      900

tcatccacac agtgttctca gtgcattgct aattactttt ttaatggtaa tttcgaagca      960

ggtaaaagtc agtgtttaaa gtgtccagta agtaaaacta ctccagcaca tgctccaggt     1020

aatactgcta ctcaggccac acagtgtttg accacatgtc ctgctggtac agtacttgat     1080

gatggaacat caactaattt tgtagcttcc gcaactgaat gtactaaatg ttctgctggc     1140

ttttttgcat caaaaacaac tggttttaca gcaggtactg atacatgtac tgaatgtact     1200

aaaaaattaa cttctggtgc cacagctaaa gtatatgctg aagctactca aaaagtacag     1260

tgcgcctcca ctactttcgc taaattttta tcgatttcct tattatttat ttctttctat     1320

ttattgtga                                                             1329


<210>  4
<211>  442
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ich IAG48 (TAA and TAG codons changed to CAG (* to Q)) PRT

<400>  4

Met Lys Tyr Asn Ile Leu Leu Ile Leu Ile Ile Ser Leu Phe Ile Asn 
1               5                   10                  15      


Glu Leu Arg Ala Val Pro Cys Pro Asp Gly Thr Gln Thr Gln Ala Gly 
            20                  25                  30          


Leu Thr Asp Val Gly Ala Ala Asp Leu Gly Thr Cys Val Asn Cys Arg 
        35                  40                  45              


Pro Asn Phe Tyr Tyr Asn Gly Gly Ala Ala Gln Gly Glu Ala Asn Gly 
    50                  55                  60                  


Asn Gln Pro Phe Ala Ala Asn Asn Ala Ala Arg Gly Ile Cys Val Pro 
65                  70                  75                  80  


Cys Gln Ile Asn Arg Val Gly Ser Val Thr Asn Ala Gly Asp Leu Ala 
                85                  90                  95      


Thr Leu Ala Thr Gln Cys Ser Thr Gln Cys Pro Thr Gly Thr Ala Leu 
            100                 105                 110         


Asp Asp Gly Val Thr Asp Val Phe Asp Arg Ser Ala Ala Gln Cys Val 
        115                 120                 125             


Lys Cys Lys Pro Asn Phe Tyr Tyr Asn Gly Gly Ser Pro Gln Gly Glu 
    130                 135                 140                 


Ala Pro Gly Val Gln Val Phe Ala Ala Gly Ala Ala Ala Ala Gly Val 
145                 150                 155                 160 


Ala Ala Val Thr Ser Gln Cys Val Pro Cys Gln Leu Asn Lys Asn Asp 
                165                 170                 175     


Ser Pro Ala Thr Ala Gly Ala Gln Ala Asn Leu Ala Thr Gln Cys Ser 
            180                 185                 190         


Asn Gln Cys Pro Thr Gly Thr Val Leu Asp Asp Gly Val Thr Leu Val 
        195                 200                 205             


Phe Asn Thr Ser Ala Thr Leu Cys Val Lys Cys Arg Pro Asn Phe Tyr 
    210                 215                 220                 


Tyr Asn Gly Gly Ser Pro Gln Gly Glu Ala Pro Gly Val Gln Val Phe 
225                 230                 235                 240 


Ala Ala Gly Ala Ala Ala Ala Gly Val Ala Ala Val Thr Ser Gln Cys 
                245                 250                 255     


Val Pro Cys Gln Ile Asn Lys Asn Asp Ser Pro Ala Thr Ala Gly Ala 
            260                 265                 270         


Gln Ala Asn Leu Ala Thr Gln Cys Ser Thr Gln Cys Pro Thr Gly Thr 
        275                 280                 285             


Ala Ile Gln Asp Gly Val Thr Leu Val Phe Ser Asn Ser Ser Thr Gln 
    290                 295                 300                 


Cys Ser Gln Cys Ile Ala Asn Tyr Phe Phe Asn Gly Asn Phe Glu Ala 
305                 310                 315                 320 


Gly Lys Ser Gln Cys Leu Lys Cys Pro Val Ser Lys Thr Thr Pro Ala 
                325                 330                 335     


His Ala Pro Gly Asn Thr Ala Thr Gln Ala Thr Gln Cys Leu Thr Thr 
            340                 345                 350         


Cys Pro Ala Gly Thr Val Leu Asp Asp Gly Thr Ser Thr Asn Phe Val 
        355                 360                 365             


Ala Ser Ala Thr Glu Cys Thr Lys Cys Ser Ala Gly Phe Phe Ala Ser 
    370                 375                 380                 


Lys Thr Thr Gly Phe Thr Ala Gly Thr Asp Thr Cys Thr Glu Cys Thr 
385                 390                 395                 400 


Lys Lys Leu Thr Ser Gly Ala Thr Ala Lys Val Tyr Ala Glu Ala Thr 
                405                 410                 415     


Gln Lys Val Gln Cys Ala Ser Thr Thr Phe Ala Lys Phe Leu Ser Ile 
            420                 425                 430         


Ser Leu Leu Phe Ile Ser Phe Tyr Leu Leu 
        435                 440         


<210>  5
<211>  1329
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ich IAG48 Codon Optimized

<400>  5
atgaagtaca acatactgtt aatacttatc atttcgcttt tcataaatga gcttagagca       60

gtgccctgcc ccgatggaac acaaacacag gccggattga cagacgttgg ggcagccgat      120

ctggggactt gtgtcaactg tcggccgaat ttttattata acggaggcgc agcgcagggt      180

gaggcgaacg gcaatcagcc ctttgcggcg aacaatgcag cgagaggcat ctgtgttcct      240

tgtcaaatca accgtgtagg cagcgtaaca aacgccgggg atcttgccac cctggccaca      300

cagtgtagca cacaatgccc tacggggacc gcattggatg atggggttac cgacgttttt      360

gatagatctg cagctcagtg cgtaaagtgt aaaccgaact tctattataa cggtggttct      420

ccacagggcg aggcccccgg ggtacaagtg tttgcggctg gtgcagccgc tgctggagtc      480

gccgccgtaa catcccaatg tgtgccctgc caactgaaca agaacgacag tcctgctacg      540

gccggagccc aggcaaacct ggctacgcaa tgttccaatc aatgccctac tgggaccgtg      600

ttggacgatg gggtaacatt ggttttcaat acgtcagcaa ctctgtgcgt taaatgtcgt      660

cccaatttct actataacgg aggtagccct cagggagaag ccccgggggt ccaggtcttc      720

gctgcaggtg ccgcagctgc gggggtggcc gcagttacat cgcaatgcgt accgtgccag      780

atcaacaaaa atgatagccc ggcgacagca ggggctcaag cgaatcttgc aacccaatgc      840

tctactcaat gcccgaccgg tacagctatc caagacggag tgaccctggt tttttctaat      900

tcctcgacac agtgttcaca gtgcatcgct aattactttt ttaacgggaa ttttgaggca      960

gggaagtcgc aatgtttaaa atgtcctgtg agtaaaacga ctcccgcaca tgcccctggg     1020

aacacagcta cgcaggcaac ccaatgcctg acgacgtgtc cggcaggtac cgtcctggac     1080

gatgggactt ctacaaattt tgtagcctcc gcgactgaat gtacaaagtg cagcgcgggt     1140

tttttcgcta gcaaaacgac ggggttcacg gcaggaacag atacttgcac ggaatgtacg     1200

aaaaaattaa cgagtggcgc gacggcgaag gtttacgcag aggcgactca gaaagtacaa     1260

tgtgcatcta caacattcgc aaagttcctt tccatctcct tgctgtttat ttcgttctac     1320

ctgctgtga                                                             1329


<210>  6
<211>  418
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ich IAG48 Codon Optimized PRT

<400>  6

Met Lys Tyr Asn Ile Leu Leu Ile Leu Ile Ile Ser Leu Phe Ile Asn 
1               5                   10                  15      


Glu Leu Arg Ala Val Pro Cys Pro Asp Gly Thr Thr Gln Ala Gly Leu 
            20                  25                  30          


Thr Asp Val Gly Ala Ala Asp Leu Gly Thr Cys Val Asn Cys Arg Pro 
        35                  40                  45              


Asn Phe Tyr Tyr Asn Gly Gly Ala Ala Gly Glu Ala Asn Gly Asn Pro 
    50                  55                  60                  


Phe Ala Ala Asn Asn Ala Ala Arg Gly Ile Cys Val Pro Cys Gln Ile 
65                  70                  75                  80  


Asn Arg Val Gly Ser Val Thr Asn Ala Gly Asp Leu Ala Thr Leu Ala 
                85                  90                  95      


Thr Cys Ser Thr Cys Pro Thr Gly Thr Ala Leu Asp Asp Gly Val Thr 
            100                 105                 110         


Asp Val Phe Asp Arg Ser Ala Ala Cys Val Lys Cys Lys Pro Asn Phe 
        115                 120                 125             


Tyr Tyr Asn Gly Gly Ser Pro Gly Glu Ala Pro Gly Val Val Phe Ala 
    130                 135                 140                 


Ala Gly Ala Ala Ala Ala Gly Val Ala Ala Val Thr Ser Cys Val Pro 
145                 150                 155                 160 


Cys Gln Leu Asn Lys Asn Asp Ser Pro Ala Thr Ala Gly Ala Ala Asn 
                165                 170                 175     


Leu Ala Thr Cys Ser Asn Cys Pro Thr Gly Thr Val Leu Asp Asp Gly 
            180                 185                 190         


Val Thr Leu Val Phe Asn Thr Ser Ala Thr Leu Cys Val Lys Cys Arg 
        195                 200                 205             


Pro Asn Phe Tyr Tyr Asn Gly Gly Ser Pro Gly Glu Ala Pro Gly Val 
    210                 215                 220                 


Val Phe Ala Ala Gly Ala Ala Ala Ala Gly Val Ala Ala Val Thr Ser 
225                 230                 235                 240 


Cys Val Pro Cys Gln Ile Asn Lys Asn Asp Ser Pro Ala Thr Ala Gly 
                245                 250                 255     


Ala Ala Asn Leu Ala Thr Cys Ser Thr Cys Pro Thr Gly Thr Ala Ile 
            260                 265                 270         


Gln Asp Gly Val Thr Leu Val Phe Ser Asn Ser Ser Thr Cys Ser Cys 
        275                 280                 285             


Ile Ala Asn Tyr Phe Phe Asn Gly Asn Phe Glu Ala Gly Lys Ser Cys 
    290                 295                 300                 


Leu Lys Cys Pro Val Ser Lys Thr Thr Pro Ala His Ala Pro Gly Asn 
305                 310                 315                 320 


Thr Ala Thr Ala Thr Cys Leu Thr Thr Cys Pro Ala Gly Thr Val Leu 
                325                 330                 335     


Asp Asp Gly Thr Ser Thr Asn Phe Val Ala Ser Ala Thr Glu Cys Thr 
            340                 345                 350         


Lys Cys Ser Ala Gly Phe Phe Ala Ser Lys Thr Thr Gly Phe Thr Ala 
        355                 360                 365             


Gly Thr Asp Thr Cys Thr Glu Cys Thr Lys Lys Leu Thr Ser Gly Ala 
    370                 375                 380                 


Thr Ala Lys Val Tyr Ala Glu Ala Thr Gln Lys Val Cys Ala Ser Thr 
385                 390                 395                 400 


Thr Phe Ala Lys Phe Leu Ser Ile Ser Leu Leu Phe Ile Ser Phe Tyr 
                405                 410                 415     


Leu Leu 
        


<210>  7
<211>  1407
<212>  DNA
<213>  Ichthyophthirius multifiliis


<220>
<221>  misc_feature
<222>  (1)..(1407)
<223>  IAG52A (GenBank: AF324424.1)

<400>  7
atgaaaaata atattttagt aatattgatt atttcattat ttatcaatta aattaaatct       60

gctaattgtc ctgttggaac tgaaactaac acagccggat aagttgatga tctaggaact      120

cctgcaaatt gtgttaattg ttagaaaaac ttttattata ataatgctgc tgctttcgtt      180

cctggtgcta gtacgtgtac accttgtcca taaaaaaaag atgctggtgc ttaaccaaat      240

ccacctgcta ctgctaattt agtcacataa tgtaacgtta aatgccctgc tggtaccgca      300

attgcaggtg gagcaacaga ttatgcagca ataatcacag aatgtgttaa ttgtagaatt      360

aatttttata atgaaaatgc tccaaatttt aatgcaggtg ctagtacatg cacagcttgt      420

ccggtaaaca gagttggtgg tgcattgact gctggtaatg ccgctaccat agtcgcataa      480

tgtaacgtcg catgtcctac tggtactgca cttgatgatg gagtaactac tgattatgtt      540

agatcattca cagaatgtgt taaatgtaga cttaactttt actataatgg taataatggt      600

aatactcctt tcaatccagg taaaagttaa tgcacacctt gtccggcaat taaacctgct      660

aatgttgctt aagctacttt aggtaatgat gctacaataa ccgcataatg taacgttgca      720

tgccctgatg gtactataag tgctgctgga gtaaataatt gggtagcaca aaacactgaa      780

tgtactaatt gtgctcctaa cttttacaat aataatgctc ctaatttcaa tccaggtaat      840

agtacatgcc taccttgccc agcaaataaa gattatggtg ctgaagccac tgcaggtggt      900

gccgctactt tagccaaata atgtaatatt gcatgccctg atggtactgc aattgctagt      960

ggagcaacta attatgtaat attataaaca gaatgtctaa attgtgctgc taacttttat     1020

tttgatggta ataatttcta ggcaggaagt agtagatgca aagcatgtcc agcaaataaa     1080

gtttaaggcg ctgtagcaac tgcaggtggt actgctactt taattgcata atgtgccctt     1140

gaatgccctg ctggtactgt actcaccgat ggaacaacat ctacttataa ataagcagca     1200

tctgaatgtg ttaaatgtgc tgccaacttt tatactacaa aataaactga ttgggtagca     1260

ggtattgata catgtactag ttgtaataaa aaattaactt ctggcgctga agctaattta     1320

cctgaatctg ctaaaaaaaa tatataatgt gatttcgcta attttttatc aatttcctta     1380

ttattgattt cttattattt attatga                                         1407


<210>  8
<211>  468
<212>  PRT
<213>  Ichthyophthirius multifiliis


<220>
<221>  MISC_FEATURE
<222>  (1)..(468)
<223>  IAG52A (GenBank: AF324424.1) protein

<400>  8

Met Lys Asn Asn Ile Leu Val Ile Leu Ile Ile Ser Leu Phe Ile Asn 
1               5                   10                  15      


Gln Ile Lys Ser Ala Asn Cys Pro Val Gly Thr Glu Thr Asn Thr Ala 
            20                  25                  30          


Gly Gln Val Asp Asp Leu Gly Thr Pro Ala Asn Cys Val Asn Cys Gln 
        35                  40                  45              


Lys Asn Phe Tyr Tyr Asn Asn Ala Ala Ala Phe Val Pro Gly Ala Ser 
    50                  55                  60                  


Thr Cys Thr Pro Cys Pro Gln Lys Lys Asp Ala Gly Ala Gln Pro Asn 
65                  70                  75                  80  


Pro Pro Ala Thr Ala Asn Leu Val Thr Gln Cys Asn Val Lys Cys Pro 
                85                  90                  95      


Ala Gly Thr Ala Ile Ala Gly Gly Ala Thr Asp Tyr Ala Ala Ile Ile 
            100                 105                 110         


Thr Glu Cys Val Asn Cys Arg Ile Asn Phe Tyr Asn Glu Asn Ala Pro 
        115                 120                 125             


Asn Phe Asn Ala Gly Ala Ser Thr Cys Thr Ala Cys Pro Val Asn Arg 
    130                 135                 140                 


Val Gly Gly Ala Leu Thr Ala Gly Asn Ala Ala Thr Ile Val Ala Gln 
145                 150                 155                 160 


Cys Asn Val Ala Cys Pro Thr Gly Thr Ala Leu Asp Asp Gly Val Thr 
                165                 170                 175     


Thr Asp Tyr Val Arg Ser Phe Thr Glu Cys Val Lys Cys Arg Leu Asn 
            180                 185                 190         


Phe Tyr Tyr Asn Gly Asn Asn Gly Asn Thr Pro Phe Asn Pro Gly Lys 
        195                 200                 205             


Ser Gln Cys Thr Pro Cys Pro Ala Ile Lys Pro Ala Asn Val Ala Gln 
    210                 215                 220                 


Ala Thr Leu Gly Asn Asp Ala Thr Ile Thr Ala Gln Cys Asn Val Ala 
225                 230                 235                 240 


Cys Pro Asp Gly Thr Ile Ser Ala Ala Gly Val Asn Asn Trp Val Ala 
                245                 250                 255     


Gln Asn Thr Glu Cys Thr Asn Cys Ala Pro Asn Phe Tyr Asn Asn Asn 
            260                 265                 270         


Ala Pro Asn Phe Asn Pro Gly Asn Ser Thr Cys Leu Pro Cys Pro Ala 
        275                 280                 285             


Asn Lys Asp Tyr Gly Ala Glu Ala Thr Ala Gly Gly Ala Ala Thr Leu 
    290                 295                 300                 


Ala Lys Gln Cys Asn Ile Ala Cys Pro Asp Gly Thr Ala Ile Ala Ser 
305                 310                 315                 320 


Gly Ala Thr Asn Tyr Val Ile Leu Gln Thr Glu Cys Leu Asn Cys Ala 
                325                 330                 335     


Ala Asn Phe Tyr Phe Asp Gly Asn Asn Phe Gln Ala Gly Ser Ser Arg 
            340                 345                 350         


Cys Lys Ala Cys Pro Ala Asn Lys Val Gln Gly Ala Val Ala Thr Ala 
        355                 360                 365             


Gly Gly Thr Ala Thr Leu Ile Ala Gln Cys Ala Leu Glu Cys Pro Ala 
    370                 375                 380                 


Gly Thr Val Leu Thr Asp Gly Thr Thr Ser Thr Tyr Lys Gln Ala Ala 
385                 390                 395                 400 


Ser Glu Cys Val Lys Cys Ala Ala Asn Phe Tyr Thr Thr Lys Gln Thr 
                405                 410                 415     


Asp Trp Val Ala Gly Ile Asp Thr Cys Thr Ser Cys Asn Lys Lys Leu 
            420                 425                 430         


Thr Ser Gly Ala Glu Ala Asn Leu Pro Glu Ser Ala Lys Lys Asn Ile 
        435                 440                 445             


Gln Cys Asp Phe Ala Asn Phe Leu Ser Ile Ser Leu Leu Leu Ile Ser 
    450                 455                 460                 


Tyr Tyr Leu Leu 
465             


<210>  9
<211>  1407
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ich IAG52A (TAA and TAG codons changed to CAG (* to Q))

<400>  9
atgaaaaata atattttagt aatattgatt atttcattat ttatcaatca gattaaatct       60

gctaattgtc ctgttggaac tgaaactaac acagccggac aggttgatga tctaggaact      120

cctgcaaatt gtgttaattg tcagaaaaac ttttattata ataatgctgc tgctttcgtt      180

cctggtgcta gtacgtgtac accttgtcca cagaaaaaag atgctggtgc tcagccaaat      240

ccacctgcta ctgctaattt agtcacacag tgtaacgtta aatgccctgc tggtaccgca      300

attgcaggtg gagcaacaga ttatgcagca ataatcacag aatgtgttaa ttgtagaatt      360

aatttttata atgaaaatgc tccaaatttt aatgcaggtg ctagtacatg cacagcttgt      420

ccggtaaaca gagttggtgg tgcattgact gctggtaatg ccgctaccat agtcgcacag      480

tgtaacgtcg catgtcctac tggtactgca cttgatgatg gagtaactac tgattatgtt      540

agatcattca cagaatgtgt taaatgtaga cttaactttt actataatgg taataatggt      600

aatactcctt tcaatccagg taaaagtcag tgcacacctt gtccggcaat taaacctgct      660

aatgttgctc aggctacttt aggtaatgat gctacaataa ccgcacagtg taacgttgca      720

tgccctgatg gtactataag tgctgctgga gtaaataatt gggtagcaca aaacactgaa      780

tgtactaatt gtgctcctaa cttttacaat aataatgctc ctaatttcaa tccaggtaat      840

agtacatgcc taccttgccc agcaaataaa gattatggtg ctgaagccac tgcaggtggt      900

gccgctactt tagccaaaca gtgtaatatt gcatgccctg atggtactgc aattgctagt      960

ggagcaacta attatgtaat attacagaca gaatgtctaa attgtgctgc taacttttat     1020

tttgatggta ataatttcca ggcaggaagt agtagatgca aagcatgtcc agcaaataaa     1080

gttcagggcg ctgtagcaac tgcaggtggt actgctactt taattgcaca gtgtgccctt     1140

gaatgccctg ctggtactgt actcaccgat ggaacaacat ctacttataa acaggcagca     1200

tctgaatgtg ttaaatgtgc tgccaacttt tatactacaa aacagactga ttgggtagca     1260

ggtattgata catgtactag ttgtaataaa aaattaactt ctggcgctga agctaattta     1320

cctgaatctg ctaaaaaaaa tatacagtgt gatttcgcta attttttatc aatttcctta     1380

ttattgattt cttattattt attatga                                         1407


<210>  10
<211>  468
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ich IAG52A (TAA and TAG codons changed to CAG (* to Q)) PRT

<400>  10

Met Lys Asn Asn Ile Leu Val Ile Leu Ile Ile Ser Leu Phe Ile Asn 
1               5                   10                  15      


Gln Ile Lys Ser Ala Asn Cys Pro Val Gly Thr Glu Thr Asn Thr Ala 
            20                  25                  30          


Gly Gln Val Asp Asp Leu Gly Thr Pro Ala Asn Cys Val Asn Cys Gln 
        35                  40                  45              


Lys Asn Phe Tyr Tyr Asn Asn Ala Ala Ala Phe Val Pro Gly Ala Ser 
    50                  55                  60                  


Thr Cys Thr Pro Cys Pro Gln Lys Lys Asp Ala Gly Ala Gln Pro Asn 
65                  70                  75                  80  


Pro Pro Ala Thr Ala Asn Leu Val Thr Gln Cys Asn Val Lys Cys Pro 
                85                  90                  95      


Ala Gly Thr Ala Ile Ala Gly Gly Ala Thr Asp Tyr Ala Ala Ile Ile 
            100                 105                 110         


Thr Glu Cys Val Asn Cys Arg Ile Asn Phe Tyr Asn Glu Asn Ala Pro 
        115                 120                 125             


Asn Phe Asn Ala Gly Ala Ser Thr Cys Thr Ala Cys Pro Val Asn Arg 
    130                 135                 140                 


Val Gly Gly Ala Leu Thr Ala Gly Asn Ala Ala Thr Ile Val Ala Gln 
145                 150                 155                 160 


Cys Asn Val Ala Cys Pro Thr Gly Thr Ala Leu Asp Asp Gly Val Thr 
                165                 170                 175     


Thr Asp Tyr Val Arg Ser Phe Thr Glu Cys Val Lys Cys Arg Leu Asn 
            180                 185                 190         


Phe Tyr Tyr Asn Gly Asn Asn Gly Asn Thr Pro Phe Asn Pro Gly Lys 
        195                 200                 205             


Ser Gln Cys Thr Pro Cys Pro Ala Ile Lys Pro Ala Asn Val Ala Gln 
    210                 215                 220                 


Ala Thr Leu Gly Asn Asp Ala Thr Ile Thr Ala Gln Cys Asn Val Ala 
225                 230                 235                 240 


Cys Pro Asp Gly Thr Ile Ser Ala Ala Gly Val Asn Asn Trp Val Ala 
                245                 250                 255     


Gln Asn Thr Glu Cys Thr Asn Cys Ala Pro Asn Phe Tyr Asn Asn Asn 
            260                 265                 270         


Ala Pro Asn Phe Asn Pro Gly Asn Ser Thr Cys Leu Pro Cys Pro Ala 
        275                 280                 285             


Asn Lys Asp Tyr Gly Ala Glu Ala Thr Ala Gly Gly Ala Ala Thr Leu 
    290                 295                 300                 


Ala Lys Gln Cys Asn Ile Ala Cys Pro Asp Gly Thr Ala Ile Ala Ser 
305                 310                 315                 320 


Gly Ala Thr Asn Tyr Val Ile Leu Gln Thr Glu Cys Leu Asn Cys Ala 
                325                 330                 335     


Ala Asn Phe Tyr Phe Asp Gly Asn Asn Phe Gln Ala Gly Ser Ser Arg 
            340                 345                 350         


Cys Lys Ala Cys Pro Ala Asn Lys Val Gln Gly Ala Val Ala Thr Ala 
        355                 360                 365             


Gly Gly Thr Ala Thr Leu Ile Ala Gln Cys Ala Leu Glu Cys Pro Ala 
    370                 375                 380                 


Gly Thr Val Leu Thr Asp Gly Thr Thr Ser Thr Tyr Lys Gln Ala Ala 
385                 390                 395                 400 


Ser Glu Cys Val Lys Cys Ala Ala Asn Phe Tyr Thr Thr Lys Gln Thr 
                405                 410                 415     


Asp Trp Val Ala Gly Ile Asp Thr Cys Thr Ser Cys Asn Lys Lys Leu 
            420                 425                 430         


Thr Ser Gly Ala Glu Ala Asn Leu Pro Glu Ser Ala Lys Lys Asn Ile 
        435                 440                 445             


Gln Cys Asp Phe Ala Asn Phe Leu Ser Ile Ser Leu Leu Leu Ile Ser 
    450                 455                 460                 


Tyr Tyr Leu Leu 
465             


<210>  11
<211>  1407
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ich IAG52A (Codon Optimized Sequence)

<400>  11
atgaagaaca acatattagt catcctgata atctcgttat tcattaatca gatcaaaagt       60

gcgaattgtc cagttggaac ggagacgaac acagccgggc aggtggatga tttaggcacg      120

cccgcaaatt gtgtaaactg ccaaaagaat ttctactata acaacgcggc agcattcgtt      180

ccaggggcgt caacttgtac gccttgtccc caaaagaagg atgctggcgc tcagcccaat      240

ccacccgcca cggcaaattt ggtaacccaa tgtaatgtaa aatgtcccgc cgggacagcg      300

atagcgggag gagcaaccga ctacgcagcc atcataacag aatgcgtcaa ctgccgcatt      360

aatttctata atgagaacgc gcccaatttc aatgcagggg ccagtacctg tactgcttgc      420

ccagtaaacc gggtgggcgg ggcgcttacg gcagggaacg ccgccacgat tgtggcacag      480

tgtaacgtag catgtccaac gggtactgcc cttgatgacg gcgtgacaac cgactatgtg      540

agatcgttta ccgagtgtgt gaaatgcaga ttgaacttct actacaacgg gaacaatgga      600

aatacgccgt ttaatccggg taaaagccaa tgcactcctt gccctgccat aaagccagcc      660

aatgtggcac aagcgactct tggtaacgac gccacaatca cagctcagtg caatgtagcg      720

tgccccgatg gtaccatctc agctgcaggt gttaataatt gggtggcaca aaacactgag      780

tgcaccaact gtgcgccgaa cttctacaat aacaacgctc cgaattttaa tccgggaaat      840

tctacgtgcc ttccatgtcc tgctaacaag gattatggcg ccgaggctac agctggcggg      900

gccgcgacgt tggccaaaca atgcaatatt gcatgccccg atggcacggc aatagctagt      960

ggggcaacga attatgtgat tttacagaca gagtgtctta actgcgcggc taatttttat     1020

ttcgatggca acaacttcca ggctggtagc tcgcgctgta aggcatgtcc agctaacaag     1080

gttcaggggg cagttgcaac cgcaggagga accgctactc ttattgccca atgtgcctta     1140

gaatgtcctg ctggcacagt attgactgat gggacgacat caacctataa gcaggcggcg     1200

agtgaatgtg tgaaatgtgc tgcgaacttc tacactacaa aacaaactga ctgggtcgcg     1260

ggtattgaca cctgcacctc atgtaataag aagttaactt ccggggctga agctaactta     1320

ccagaatcgg ctaagaaaaa tattcaatgc gacttcgcta acttcttaag tataagtctg     1380

cttttgattt cttattatct gctttga                                         1407


<210>  12
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ich IAG52A (Codon Optimized Sequence) PRT

<400>  12

Met Lys Asn Asn Ile Leu Val Ile Leu Ile Ile Ser Leu Phe Ile Asn 
1               5                   10                  15      


Ile Lys Ser Ala Asn Cys Pro Val Gly Thr Glu Thr Asn Thr Ala Gly 
            20                  25                  30          


Val Asp Asp Leu Gly Thr Pro Ala Asn Cys Val Asn Cys Lys Asn Phe 
        35                  40                  45              


Tyr Tyr Asn Asn Ala Ala Ala Phe Val Pro Gly Ala Ser Thr Cys Thr 
    50                  55                  60                  


Pro Cys Pro Lys Lys Asp Ala Gly Ala Pro Asn Pro Pro Ala Thr Ala 
65                  70                  75                  80  


Asn Leu Val Thr Cys Asn Val Lys Cys Pro Ala Gly Thr Ala Ile Ala 
                85                  90                  95      


Gly Gly Ala Thr Asp Tyr Ala Ala Ile Ile Thr Glu Cys Val Asn Cys 
            100                 105                 110         


Arg Ile Asn Phe Tyr Asn Glu Asn Ala Pro Asn Phe Asn Ala Gly Ala 
        115                 120                 125             


Ser Thr Cys Thr Ala Cys Pro Val Asn Arg Val Gly Gly Ala Leu Thr 
    130                 135                 140                 


Ala Gly Asn Ala Ala Thr Ile Val Ala Cys Asn Val Ala Cys Pro Thr 
145                 150                 155                 160 


Gly Thr Ala Leu Asp Asp Gly Val Thr Thr Asp Tyr Val Arg Ser Phe 
                165                 170                 175     


Thr Glu Cys Val Lys Cys Arg Leu Asn Phe Tyr Tyr Asn Gly Asn Asn 
            180                 185                 190         


Gly Asn Thr Pro Phe Asn Pro Gly Lys Ser Cys Thr Pro Cys Pro Ala 
        195                 200                 205             


Ile Lys Pro Ala Asn Val Ala Ala Thr Leu Gly Asn Asp Ala Thr Ile 
    210                 215                 220                 


Thr Ala Cys Asn Val Ala Cys Pro Asp Gly Thr Ile Ser Ala Ala Gly 
225                 230                 235                 240 


Val Asn Asn Trp Val Ala Gln Asn Thr Glu Cys Thr Asn Cys Ala Pro 
                245                 250                 255     


Asn Phe Tyr Asn Asn Asn Ala Pro Asn Phe Asn Pro Gly Asn Ser Thr 
            260                 265                 270         


Cys Leu Pro Cys Pro Ala Asn Lys Asp Tyr Gly Ala Glu Ala Thr Ala 
        275                 280                 285             


Gly Gly Ala Ala Thr Leu Ala Lys Cys Asn Ile Ala Cys Pro Asp Gly 
    290                 295                 300                 


Thr Ala Ile Ala Ser Gly Ala Thr Asn Tyr Val Ile Leu Thr Glu Cys 
305                 310                 315                 320 


Leu Asn Cys Ala Ala Asn Phe Tyr Phe Asp Gly Asn Asn Phe Ala Gly 
                325                 330                 335     


Ser Ser Arg Cys Lys Ala Cys Pro Ala Asn Lys Val Gly Ala Val Ala 
            340                 345                 350         


Thr Ala Gly Gly Thr Ala Thr Leu Ile Ala Cys Ala Leu Glu Cys Pro 
        355                 360                 365             


Ala Gly Thr Val Leu Thr Asp Gly Thr Thr Ser Thr Tyr Lys Ala Ala 
    370                 375                 380                 


Ser Glu Cys Val Lys Cys Ala Ala Asn Phe Tyr Thr Thr Lys Thr Asp 
385                 390                 395                 400 


Trp Val Ala Gly Ile Asp Thr Cys Thr Ser Cys Asn Lys Lys Leu Thr 
                405                 410                 415     


Ser Gly Ala Glu Ala Asn Leu Pro Glu Ser Ala Lys Lys Asn Ile Cys 
            420                 425                 430         


Asp Phe Ala Asn Phe Leu Ser Ile Ser Leu Leu Leu Ile Ser Tyr Tyr 
        435                 440                 445             


Leu Leu 
    450 


<210>  13
<211>  1266
<212>  DNA
<213>  Ichthyophthirius multifiliis


<220>
<221>  misc_feature
<222>  (1)..(1266)
<223>  IAG52B

<400>  13
atggtgaatt gcccgaatgg cgccgccatc gccaatggcc agagcgatac cggcgccgcc       60

gatatcaata cctgcaccca ttgccagaaa catttttatt ttaatggcgg caatccggcc      120

ggccaggccc cgggcgccgt gcagtttaat ccgggcgtga gccagtgcat cgcctgccag      180

gtgcataaag ccgatagcca gcatcgccag ggcggcgatg ccaatctggc cgcccagtgc      240

agcaatctgt gcccggccgg caccgccgtg gaggatggca gcccgacctt tacccagagc      300

ctgacccagt gcgtgaattg caaaccgaat ttttatttta atggcggcaa tccgaccggc      360

caggccccgg gcgccggcca gtttgatccg acccagctga tcgccaatcc ggatctggcc      420

aataatccgg aggtgccgaa tgtgagcagc ccgaatggcc agtgcgtggc ctgccaggtg      480

aataaaagcg atagccagct gcgcccgggc gcccaggcca atctggccac ccagtgcaat      540

aatgagtgcc cgaccggcac cgccatccag gatggcgcca tctttatcta tacccagagc      600

atcagccagt gcaccttttg caaagtggat ttttatttta atggcggcaa tccgagcgcc      660

cagaatccgg gcaatggcca gtttaccccg ggccagctga tcgccaatcc ggatgccgcc      720

accgccgccc agatcccgat ggtgccgggc ccgaatagca aatgcgtggc ctgcgagagc      780

aaaaaaacca atagccagag ccgcagcggc ctggaggcca atctggccgc ccagtgcggc      840

accgagtgcc cggccggcac cctggtgacc gatggcgtga ccccgaccta taccgtgagc      900

ctgagccagt gcgtgaattg caaagccggc ttttatcaga atagcaattt tgaggccggc      960

aaaagccagt gcaataaatg cgccgtgagc aaaaccggca gcgccagcgt gccgggcaat     1020

agcgccacca gcgccaccca gtgccagaat gattgcccgg ccggcaccgt ggtggatgat     1080

ggcagcacca attttgtggc cctggccagc gagtgcacca aatgccaggc caatttttat     1140

gccagcaaaa ccagcggctt tgccgccggc accgatacct gcaccgagtg cagcaaaaaa     1200

ctgaccagcg gcgccaccgc caaagtgtat gccgaggcca cccagaaagc ccagtgcgcc     1260

agctga                                                                1266


<210>  14
<211>  422
<212>  PRT
<213>  Ichthyophthirius multifiliis


<220>
<221>  MISC_FEATURE
<222>  (1)..(422)
<223>  IAG52B

<400>  14

Met Val Asn Cys Pro Asn Gly Ala Ala Ile Ala Asn Gly Gln Ser Asp 
1               5                   10                  15      


Thr Gly Ala Ala Asp Ile Asn Thr Cys Thr His Cys Gln Lys His Phe 
            20                  25                  30          


Tyr Phe Asn Gly Gly Asn Pro Ala Gly Gln Ala Pro Gly Ala Val Gln 
        35                  40                  45              


Phe Asn Pro Gly Val Ser Gln Cys Ile Ala Cys Gln Val His Lys Ala 
    50                  55                  60                  


Asp Ser Gln His Arg Gln Gly Gly Asp Ala Asn Leu Ala Ala Gln Cys 
65                  70                  75                  80  


Ser Asn Leu Cys Pro Ala Gly Thr Ala Val Glu Asp Gly Ser Pro Thr 
                85                  90                  95      


Phe Thr Gln Ser Leu Thr Gln Cys Val Asn Cys Lys Pro Asn Phe Tyr 
            100                 105                 110         


Phe Asn Gly Gly Asn Pro Thr Gly Gln Ala Pro Gly Ala Gly Gln Phe 
        115                 120                 125             


Asp Pro Thr Gln Leu Ile Ala Asn Pro Asp Leu Ala Asn Asn Pro Glu 
    130                 135                 140                 


Val Pro Asn Val Ser Ser Pro Asn Gly Gln Cys Val Ala Cys Gln Val 
145                 150                 155                 160 


Asn Lys Ser Asp Ser Gln Leu Arg Pro Gly Ala Gln Ala Asn Leu Ala 
                165                 170                 175     


Thr Gln Cys Asn Asn Glu Cys Pro Thr Gly Thr Ala Ile Gln Asp Gly 
            180                 185                 190         


Ala Ile Phe Ile Tyr Thr Gln Ser Ile Ser Gln Cys Thr Phe Cys Lys 
        195                 200                 205             


Val Asp Phe Tyr Phe Asn Gly Gly Asn Pro Ser Ala Gln Asn Pro Gly 
    210                 215                 220                 


Asn Gly Gln Phe Thr Pro Gly Gln Leu Ile Ala Asn Pro Asp Ala Ala 
225                 230                 235                 240 


Thr Ala Ala Gln Ile Pro Met Val Pro Gly Pro Asn Ser Lys Cys Val 
                245                 250                 255     


Ala Cys Glu Ser Lys Lys Thr Asn Ser Gln Ser Arg Ser Gly Leu Glu 
            260                 265                 270         


Ala Asn Leu Ala Ala Gln Cys Gly Thr Glu Cys Pro Ala Gly Thr Leu 
        275                 280                 285             


Val Thr Asp Gly Val Thr Pro Thr Tyr Thr Val Ser Leu Ser Gln Cys 
    290                 295                 300                 


Val Asn Cys Lys Ala Gly Phe Tyr Gln Asn Ser Asn Phe Glu Ala Gly 
305                 310                 315                 320 


Lys Ser Gln Cys Asn Lys Cys Ala Val Ser Lys Thr Gly Ser Ala Ser 
                325                 330                 335     


Val Pro Gly Asn Ser Ala Thr Ser Ala Thr Gln Cys Gln Asn Asp Cys 
            340                 345                 350         


Pro Ala Gly Thr Val Val Asp Asp Gly Thr Ser Thr Asn Phe Val Ala 
        355                 360                 365             


Leu Ala Ser Glu Cys Thr Lys Cys Gln Ala Asn Phe Tyr Ala Ser Lys 
    370                 375                 380                 


Thr Ser Gly Phe Ala Ala Gly Thr Asp Thr Cys Thr Glu Cys Ser Lys 
385                 390                 395                 400 


Lys Leu Thr Ser Gly Ala Thr Ala Lys Val Tyr Ala Glu Ala Thr Gln 
                405                 410                 415     


Lys Ala Gln Cys Ala Ser 
            420         


<210>  15
<211>  1263
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ich IAG52B (Codon Optimized)

<400>  15
gttaattgtc ctaatggtgc tgcaattgcg aatggataat ctgatacagg agctgcagat       60

ataaatactt gtactcattg ctaaaaacac ttttacttta atggtggtaa tcctgcaggt      120

caggctcctg gtgctgtaca attcaatcca ggtgttagtc agtgcatagc ttgccaagta      180

cacaaagccg attctcaaca cagataaggt ggtgatgcta atttagccgc ataatgtagc      240

aacttatgtc ctgctggcac tgcagttgaa gatggatcac ctacttttac ttaatccctc      300

acataatgtg ttaattgtaa acctaacttt tactttaatg gtggtaatcc tacaggtcag      360

gctcctggtg ctggataatt cgatccaact taattgattg caaatcctga tcttgctaat      420

aatcctgaag ttcctaatgt ttctagccct aatggttaat gcgtagcttg ctaagtaaac      480

aagtctgatt ctcaattaag accaggtgct taggctaatt tagccacata atgtaacaat      540

gaatgtccta ctggcactgc tattcaagac ggagcaatat ttatttatac ttaatcaatc      600

tcataatgta ctttttgtaa agttgacttt tactttaatg gtggcaatcc ttcagctcag      660

aatcctggta atggataatt cactccaggt taattgattg caaatcctga tgctgctact      720

gctgcttaaa ttcctatggt tcctggccct aatagtaaat gcgtagcttg cgaatcaaaa      780

aagaccaatt cttaatccag atcaggtctt gaggctaatt tagccgcata atgtggcact      840

gaatgtcctg ctggcactct tgttacagac ggagtaacac ctacttatac tgtatcactc      900

tcataatgtg ttaattgtaa agctggcttt tactaaaata gtaatttcga agcaggtaaa      960

agttaatgca ataagtgtgc agtaagtaaa actggttcag catctgttcc aggtaatagt     1020

gctacttcag ccacataatg ttaaaacgat tgccctgctg gtacagtggt tgatgatggt     1080

acatcaacta attttgtagc tttagcaagt gaatgtacta aatgttaggc taacttttat     1140

gcatcaaaaa catctggttt tgcagcaggt actgatacat gtactgaatg ttctaaaaaa     1200

ttaacttctg gtgctacagc taaagtatat gctgaagcta cttaaaaagc ataatgcgcc     1260

agt                                                                   1263


<210>  16
<211>  394
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ich IAG52B (Codon Optimized)

<400>  16

Val Asn Cys Pro Asn Gly Ala Ala Ile Ala Asn Gly Ser Asp Thr Gly 
1               5                   10                  15      


Ala Ala Asp Ile Asn Thr Cys Thr His Cys Lys His Phe Tyr Phe Asn 
            20                  25                  30          


Gly Gly Asn Pro Ala Gly Gln Ala Pro Gly Ala Val Gln Phe Asn Pro 
        35                  40                  45              


Gly Val Ser Gln Cys Ile Ala Cys Gln Val His Lys Ala Asp Ser Gln 
    50                  55                  60                  


His Arg Gly Gly Asp Ala Asn Leu Ala Ala Cys Ser Asn Leu Cys Pro 
65                  70                  75                  80  


Ala Gly Thr Ala Val Glu Asp Gly Ser Pro Thr Phe Thr Ser Leu Thr 
                85                  90                  95      


Cys Val Asn Cys Lys Pro Asn Phe Tyr Phe Asn Gly Gly Asn Pro Thr 
            100                 105                 110         


Gly Gln Ala Pro Gly Ala Gly Phe Asp Pro Thr Leu Ile Ala Asn Pro 
        115                 120                 125             


Asp Leu Ala Asn Asn Pro Glu Val Pro Asn Val Ser Ser Pro Asn Gly 
    130                 135                 140                 


Cys Val Ala Cys Val Asn Lys Ser Asp Ser Gln Leu Arg Pro Gly Ala 
145                 150                 155                 160 


Ala Asn Leu Ala Thr Cys Asn Asn Glu Cys Pro Thr Gly Thr Ala Ile 
                165                 170                 175     


Gln Asp Gly Ala Ile Phe Ile Tyr Thr Ser Ile Ser Cys Thr Phe Cys 
            180                 185                 190         


Lys Val Asp Phe Tyr Phe Asn Gly Gly Asn Pro Ser Ala Gln Asn Pro 
        195                 200                 205             


Gly Asn Gly Phe Thr Pro Gly Leu Ile Ala Asn Pro Asp Ala Ala Thr 
    210                 215                 220                 


Ala Ala Ile Pro Met Val Pro Gly Pro Asn Ser Lys Cys Val Ala Cys 
225                 230                 235                 240 


Glu Ser Lys Lys Thr Asn Ser Ser Arg Ser Gly Leu Glu Ala Asn Leu 
                245                 250                 255     


Ala Ala Cys Gly Thr Glu Cys Pro Ala Gly Thr Leu Val Thr Asp Gly 
            260                 265                 270         


Val Thr Pro Thr Tyr Thr Val Ser Leu Ser Cys Val Asn Cys Lys Ala 
        275                 280                 285             


Gly Phe Tyr Asn Ser Asn Phe Glu Ala Gly Lys Ser Cys Asn Lys Cys 
    290                 295                 300                 


Ala Val Ser Lys Thr Gly Ser Ala Ser Val Pro Gly Asn Ser Ala Thr 
305                 310                 315                 320 


Ser Ala Thr Cys Asn Asp Cys Pro Ala Gly Thr Val Val Asp Asp Gly 
                325                 330                 335     


Thr Ser Thr Asn Phe Val Ala Leu Ala Ser Glu Cys Thr Lys Cys Ala 
            340                 345                 350         


Asn Phe Tyr Ala Ser Lys Thr Ser Gly Phe Ala Ala Gly Thr Asp Thr 
        355                 360                 365             


Cys Thr Glu Cys Ser Lys Lys Leu Thr Ser Gly Ala Thr Ala Lys Val 
    370                 375                 380                 


Tyr Ala Glu Ala Thr Lys Ala Cys Ala Ser 
385                 390                 


