                         SEQUENCE LISTING

<110>  The Secretary of State for Health
 
<120>  Lassa Virus Antigenic Composition

<130>  P50886WO

<150>  GB 1801437.3
<151>  2018-01-29

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  1710
<212>  DNA
<213>  Lassa virus

<400>  1
atgagtgctt ccaaggaagt gaggtcattc ttgtggactc aatccctaag aagggaacta       60

tctggctact gttccaacat aaagttgcag gtagttaaag acgctcaagc tctccttcat      120

ggtcttgact tctccgaggt cagtaatgtt cagagattga tgcgcaagca gaaaagagat      180

gatggcgacc taaaacgact gagagatctc aatcaagcag tcaacaatct tgttgagctc      240

aaatccacac agcagaaaag tgtcttaaga gttggaacct taagttcaga cgatctacta      300

atcctggccg ctgatttaga aaaactgaaa tcaaaagtca ccagaacaga aaggcctttg      360

agttcaggag tttatatggg gaatttgagt tcacaacagc ttgatcaaag gagagccctt      420

ttgaacatga ttggcatgac tggagtaagt ggagggggaa agggtgccag tgatggcatt      480

gtgagagttt gggatgtcaa aaatgcagag ttactcaaca atcagttcgg aacaatgcca      540

agcctaactt tagcatgcct gaccaaacag gggcaagtgg acctgaatga tgccgttcaa      600

gctttgacag atttagggct gatttacaca gccaaatacc ccaactcatc tgatctcgac      660

agattgtctc agagtcatcc aattctgaat atgattgaca ctaagaaaag ttcactcaac      720

atctctggtt acaatttcag tttgggtgct gctgtcaaag caggggcctg catgcttgat      780

ggtggtaaca tgttagagac tattaaggtt tcacctcaga ccatggatgg tatcttgaag      840

tcaatcttga aagttaagaa gagtctggga atgtttgtat cagacacacc gggtgaaagg      900

aacccttatg agaacatcct atacaagatc tgtctctcag gagacggatg gccctatatt      960

gcatcaagga cctcgattgt gggaagagca tgggaaaata ctgtggtgga ccttgagcaa     1020

gacaacaagc cccagaaaat tggaaatggg gggtccaaca agtcattaca gtctgcaggc     1080

tttgctgcag gattaactta ctctcagttg atgactctca aagatttcaa gtgcttcaac     1140

ttgattccca acgcaaaaac ctggatggat attgaaggaa gaccagaaga cccagttgag     1200

atagcccttt atcaaccgag ctcgggttgc tatgtacatt tctttaggga gccaacagat     1260

ttgaagcaat tcaaacaaga tgcaaagtat tcacatggta ttgatgtgac tgatttgttt     1320

gctgcccaac ctgggttaac cagtgcagtg atagaagccc ttcctcggaa catggtcatc     1380

acttgccaag gatcagagga tatcagaaaa ctccttgagt cacaagggag gagagacata     1440

aaactgattg acatcactct tagtaaagca gattcaagaa agtttgagaa tgctgtttgg     1500

gatcaattca aggatctatg tcacatgcac actggggtag ttgtggagaa aaagaagaga     1560

ggtggtaaag aggaaataac tcctcattgt gcactgatgg attgcattat gtttgatgca     1620

gcagtttcag gaggacttga tgcaaaagtc ctgagagctg tgctccctag agacatggtg     1680

ttcagaactt caacacctaa agtcgtcctg                                      1710


<210>  2
<211>  570
<212>  PRT
<213>  Lassa virus

<400>  2

Met Ser Ala Ser Lys Glu Val Arg Ser Phe Leu Trp Thr Gln Ser Leu 
1               5                   10                  15      


Arg Arg Glu Leu Ser Gly Tyr Cys Ser Asn Ile Lys Leu Gln Val Val 
            20                  25                  30          


Lys Asp Ala Gln Ala Leu Leu His Gly Leu Asp Phe Ser Glu Val Ser 
        35                  40                  45              


Asn Val Gln Arg Leu Met Arg Lys Gln Lys Arg Asp Asp Gly Asp Leu 
    50                  55                  60                  


Lys Arg Leu Arg Asp Leu Asn Gln Ala Val Asn Asn Leu Val Glu Leu 
65                  70                  75                  80  


Lys Ser Thr Gln Gln Lys Ser Val Leu Arg Val Gly Thr Leu Ser Ser 
                85                  90                  95      


Asp Asp Leu Leu Ile Leu Ala Ala Asp Leu Glu Lys Leu Lys Ser Lys 
            100                 105                 110         


Val Thr Arg Thr Glu Arg Pro Leu Ser Ser Gly Val Tyr Met Gly Asn 
        115                 120                 125             


Leu Ser Ser Gln Gln Leu Asp Gln Arg Arg Ala Leu Leu Asn Met Ile 
    130                 135                 140                 


Gly Met Thr Gly Val Ser Gly Gly Gly Lys Gly Ala Ser Asp Gly Ile 
145                 150                 155                 160 


Val Arg Val Trp Asp Val Lys Asn Ala Glu Leu Leu Asn Asn Gln Phe 
                165                 170                 175     


Gly Thr Met Pro Ser Leu Thr Leu Ala Cys Leu Thr Lys Gln Gly Gln 
            180                 185                 190         


Val Asp Leu Asn Asp Ala Val Gln Ala Leu Thr Asp Leu Gly Leu Ile 
        195                 200                 205             


Tyr Thr Ala Lys Tyr Pro Asn Ser Ser Asp Leu Asp Arg Leu Ser Gln 
    210                 215                 220                 


Ser His Pro Ile Leu Asn Met Ile Asp Thr Lys Lys Ser Ser Leu Asn 
225                 230                 235                 240 


Ile Ser Gly Tyr Asn Phe Ser Leu Gly Ala Ala Val Lys Ala Gly Ala 
                245                 250                 255     


Cys Met Leu Asp Gly Gly Asn Met Leu Glu Thr Ile Lys Val Ser Pro 
            260                 265                 270         


Gln Thr Met Asp Gly Ile Leu Lys Ser Ile Leu Lys Val Lys Lys Ser 
        275                 280                 285             


Leu Gly Met Phe Val Ser Asp Thr Pro Gly Glu Arg Asn Pro Tyr Glu 
    290                 295                 300                 


Asn Ile Leu Tyr Lys Ile Cys Leu Ser Gly Asp Gly Trp Pro Tyr Ile 
305                 310                 315                 320 


Ala Ser Arg Thr Ser Ile Val Gly Arg Ala Trp Glu Asn Thr Val Val 
                325                 330                 335     


Asp Leu Glu Gln Asp Asn Lys Pro Gln Lys Ile Gly Asn Gly Gly Ser 
            340                 345                 350         


Asn Lys Ser Leu Gln Ser Ala Gly Phe Ala Ala Gly Leu Thr Tyr Ser 
        355                 360                 365             


Gln Leu Met Thr Leu Lys Asp Phe Lys Cys Phe Asn Leu Ile Pro Asn 
    370                 375                 380                 


Ala Lys Thr Trp Met Asp Ile Glu Gly Arg Pro Glu Asp Pro Val Glu 
385                 390                 395                 400 


Ile Ala Leu Tyr Gln Pro Ser Ser Gly Cys Tyr Val His Phe Phe Arg 
                405                 410                 415     


Glu Pro Thr Asp Leu Lys Gln Phe Lys Gln Asp Ala Lys Tyr Ser His 
            420                 425                 430         


Gly Ile Asp Val Thr Asp Leu Phe Ala Ala Gln Pro Gly Leu Thr Ser 
        435                 440                 445             


Ala Val Ile Glu Ala Leu Pro Arg Asn Met Val Ile Thr Cys Gln Gly 
    450                 455                 460                 


Ser Glu Asp Ile Arg Lys Leu Leu Glu Ser Gln Gly Arg Arg Asp Ile 
465                 470                 475                 480 


Lys Leu Ile Asp Ile Thr Leu Ser Lys Ala Asp Ser Arg Lys Phe Glu 
                485                 490                 495     


Asn Ala Val Trp Asp Gln Phe Lys Asp Leu Cys His Met His Thr Gly 
            500                 505                 510         


Val Val Val Glu Lys Lys Lys Arg Gly Gly Lys Glu Glu Ile Thr Pro 
        515                 520                 525             


His Cys Ala Leu Met Asp Cys Ile Met Phe Asp Ala Ala Val Ser Gly 
    530                 535                 540                 


Gly Leu Asp Ala Lys Val Leu Arg Ala Val Leu Pro Arg Asp Met Val 
545                 550                 555                 560 


Phe Arg Thr Ser Thr Pro Lys Val Val Leu 
                565                 570 


<210>  3
<211>  3417
<212>  DNA
<213>  Lassa virus

<400>  3
cgcacagtgg atcctaggct attggattgc gctttgcact aatcaaacct ttggagtgac       60

cacattcaag acccactcaa gggtcagaca accatcacga caatgagtgc ttccaaggaa      120

gtgaggtcat tcttgtggac tcaatcccta agaagggaac tatctggcta ctgttccaac      180

ataaagttgc aggtagttaa agacgctcaa gctctccttc atggtcttga cttctccgag      240

gtcagtaatg ttcagagatt gatgcgcaag cagaaaagag atgatggcga cctaaaacga      300

ctgagagatc tcaatcaagc agtcaacaat cttgttgagc tcaaatccac acagcagaaa      360

agtgtcttaa gagttggaac cttaagttca gacgatctac taatcctggc cgctgattta      420

gaaaaactga aatcaaaagt caccagaaca gaaaggcctt tgagttcagg agtttatatg      480

gggaatttga gttcacaaca gcttgatcaa aggagagccc ttttgaacat gattggcatg      540

actggagtaa gtggaggggg aaagggtgcc agtgatggca ttgtgagagt ttgggatgtc      600

aaaaatgcag agttactcaa caatcagttc ggaacaatgc caagcctaac tttagcatgc      660

ctgaccaaac aggggcaagt ggacctgaat gatgccgttc aagctttgac agatttaggg      720

ctgatttaca cagccaaata ccccaactca tctgatctcg acagattgtc tcagagtcat      780

ccaattctga atatgattga cactaagaaa agttcactca acatctctgg ttacaatttc      840

agtttgggtg ctgctgtcaa agcaggggcc tgcatgcttg atggtggtaa catgttagag      900

actattaagg tttcacctca gaccatggat ggtatcttga agtcaatctt gaaagttaag      960

aagagtctgg gaatgtttgt atcagacaca ccgggtgaaa ggaaccctta tgagaacatc     1020

ctatacaaga tctgtctctc aggagacgga tggccctata ttgcatcaag gacctcgatt     1080

gtgggaagag catgggaaaa tactgtggtg gaccttgagc aagacaacaa gccccagaaa     1140

attggaaatg gggggtccaa caagtcatta cagtctgcag gctttgctgc aggattaact     1200

tactctcagt tgatgactct caaagatttc aagtgcttca acttgattcc caacgcaaaa     1260

acctggatgg atattgaagg aagaccagaa gacccagttg agatagccct ttatcaaccg     1320

agctcgggtt gctatgtaca tttctttagg gagccaacag atttgaagca attcaaacaa     1380

gatgcaaagt attcacatgg tattgatgtg actgatttgt ttgctgccca acctgggtta     1440

accagtgcag tgatagaagc ccttcctcgg aacatggtca tcacttgcca aggatcagag     1500

gatatcagaa aactccttga gtcacaaggg aggagagaca taaaactgat tgacatcact     1560

cttagtaaag cagattcaag aaagtttgag aatgctgttt gggatcaatt caaggatcta     1620

tgtcacatgc acactggggt agttgtggag aaaaagaaga gaggtggtaa agaggaaata     1680

actcctcatt gtgcactgat ggattgcatt atgtttgatg cagcagtttc aggaggactt     1740

gatgcaaaag tcctgagagt tgtgctccct agagacatgg tgttcagaac ttcaacacct     1800

aaagtcgtcc tgtagatgga cgcccccgtg acccaccgcc aattggcggt gggtcacggg     1860

ggccctgtgg gatttcacct cttccatctg acaggcacac ctggctgttt gtatagacca     1920

caggagcaga tgcccatgtg gttcagcctg tggggttttg ggcaaggttt acctacaatg     1980

tgtctatggg ttggtatttt gaccaggtgg agaaagatgc ttatcagata gaaacttgtg     2040

ctaaaaacaa ataggtcaac taaccccagt ggagttttgc cctgtctatc tatgtattcc     2100

ttttgtagca tctctgttat catgttgtca gcttgttgtt cgatgtcatc tgaaaacttg     2160

gtctcattga gatatgatcc atttgagata agccaacacc ttggtagtga ggtctttcct     2220

gttgatgtgt ggttaaggta ccaatatctg ctgtagttac aatatggtat gcccatgatg     2280

tctctcaagt ggttcttcat gatgagctga tcatttatta aagcattgac agccttgttg     2340

atcagctgta tgctcatttg ggcctctgtt ttgagcctcc ttatggcctg tttgttgaaa     2400

tcgaacaacc ttagcatgtc acaaaattct tcatcatgtt tctcattgca cttggctact     2460

gcagtgtttc caaagcactt taattcggcc tcaatcaaca tccatctagt aaggcagtat     2520

ccccctggtg tttcatttcc ctctgagtct gatagtgtcc aggtgaatgt ccccaacaac     2580

cttctgctaa tgtatatgtc tctagttctt tgtgagagaa gcccaaggta gccaataggt     2640

gatggtctgg aaaattggca gtggtcatcc caggttgtat tctgaatgat tagatattgg     2700

taactggtca ttatacagtc ccagttaccg cgtccagaat caagagcaat ataactccct     2760

ccccaagcca ttctcataaa tgtttgtaag acaccatttg caagtgtgcc gcagtgacct     2820

gctgcatcca ctgcgaagct atgactcagg ttgtattgca cagtgatttt ccccccattg     2880

aaatcgcagc tcattgcctc atattgattg aagttgggaa tggacagatg aaaggtagag     2940

atgatactca tgaggctgtg atcataaaga ttccttttgt gggcatcaga gaggttacag     3000

aatttgtgat taagaatgct ggtgttggtc aaggtgagct caagtcctgt ctcattcccc     3060

acccttatat aatgatgact gttgttcttt gtgcaggata gcggcatggt catattaaga     3120

gtctccatat tcaactcaag ggtttgcagc tcgtaagtcc ctttgtagat cagtgagcat     3180

gaccttcctg aaagtagaag gaatgtgaca agccctatca agccacacgt ggcaacattg     3240

tatagtccct tcagaattgc taggatggat agtgcaataa ggacaatatt catcacttcc     3300

tcaataacat gaggaacttc ttggaagaat gtcacaatct gtcccatcct gattgcgaca     3360

ctttccaaaa aggaggtttt aaaatgcgca atcctaaatg cctaggatcc ccggtgc        3417


<210>  4
<211>  1710
<212>  DNA
<213>  Lassa virus

<400>  4
atgagtgctt ccaaggaagt gaggtcattc ttgtggactc aatccctaag aagggaacta       60

tctggctact gttccaacat aaagttgcag gtagttaaag acgctcaagc tctccttcat      120

ggtcttgact tctccgaggt cagtaatgtt cagagattga tgcgcaagca gaaaagagat      180

gatggcgacc taaaacgact gagagatctc aatcaagcag tcaacaatct tgttgagctc      240

aaatccacac agcagaaaag tgtcttaaga gttggaacct taagttcaga cgatctacta      300

atcctggccg ctgatttaga aaaactgaaa tcaaaagtca ccagaacaga aaggcctttg      360

agttcaggag tttatatggg gaatttgagt tcacaacagc ttgatcaaag gagagccctt      420

ttgaacatga ttggcatgac tggagtaagt ggagggggaa agggtgccag tgatggcatt      480

gtgagagttt gggatgtcaa aaatgcagag ttactcaaca atcagttcgg aacaatgcca      540

agcctaactt tagcatgcct gaccaaacag gggcaagtgg acctgaatga tgccgttcaa      600

gctttgacag atttagggct gatttacaca gccaaatacc ccaactcatc tgatctcgac      660

agattgtctc agagtcatcc aattctgaat atgattgaca ctaagaaaag ttcactcaac      720

atctctggtt acaatttcag tttgggtgct gctgtcaaag caggggcctg catgcttgat      780

ggtggtaaca tgttagagac tattaaggtt tcacctcaga ccatggatgg tatcttgaag      840

tcaatcttga aagttaagaa gagtctggga atgtttgtat cagacacacc gggtgaaagg      900

aacccttatg agaacatcct atacaagatc tgtctctcag gagacggatg gccctatatt      960

gcatcaagga cctcgattgt gggaagagca tgggaaaata ctgtggtgga ccttgagcaa     1020

gacaacaagc cccagaaaat tggaaatggg gggtccaaca agtcattaca gtctgcaggc     1080

tttgctgcag gattaactta ctctcagttg atgactctca aagatttcaa gtgcttcaac     1140

ttgattccca acgcaaaaac ctggatggat attgaaggaa gaccagaaga cccagttgag     1200

atagcccttt atcaaccgag ctcgggttgc tatgtacatt tctttaggga gccaacagat     1260

ttgaagcaat tcaaacaaga tgcaaagtat tcacatggta ttgatgtgac tgatttgttt     1320

gctgcccaac ctgggttaac cagtgcagtg atagaagccc ttcctcggaa catggtcatc     1380

acttgccaag gatcagagga tatcagaaaa ctccttgagt cacaagggag gagagacata     1440

aaactgattg acatcactct tagtaaagca gattcaagaa agtttgagaa tgctgtttgg     1500

gatcaattca aggatctatg tcacatgcac actggggtag ttgtggagaa aaagaagaga     1560

ggtggtaaag aggaaataac tcctcattgt gcactgatgg attgcattat gtttgatgca     1620

gcagtttcag gaggacttga tgcaaaagtc ctgagagttg tgctccctag agacatggtg     1680

ttcagaactt caacacctaa agtcgtcctg                                      1710


<210>  5
<211>  570
<212>  PRT
<213>  Lassa virus

<400>  5

Met Ser Ala Ser Lys Glu Val Arg Ser Phe Leu Trp Thr Gln Ser Leu 
1               5                   10                  15      


Arg Arg Glu Leu Ser Gly Tyr Cys Ser Asn Ile Lys Leu Gln Val Val 
            20                  25                  30          


Lys Asp Ala Gln Ala Leu Leu His Gly Leu Asp Phe Ser Glu Val Ser 
        35                  40                  45              


Asn Val Gln Arg Leu Met Arg Lys Gln Lys Arg Asp Asp Gly Asp Leu 
    50                  55                  60                  


Lys Arg Leu Arg Asp Leu Asn Gln Ala Val Asn Asn Leu Val Glu Leu 
65                  70                  75                  80  


Lys Ser Thr Gln Gln Lys Ser Val Leu Arg Val Gly Thr Leu Ser Ser 
                85                  90                  95      


Asp Asp Leu Leu Ile Leu Ala Ala Asp Leu Glu Lys Leu Lys Ser Lys 
            100                 105                 110         


Val Thr Arg Thr Glu Arg Pro Leu Ser Ser Gly Val Tyr Met Gly Asn 
        115                 120                 125             


Leu Ser Ser Gln Gln Leu Asp Gln Arg Arg Ala Leu Leu Asn Met Ile 
    130                 135                 140                 


Gly Met Thr Gly Val Ser Gly Gly Gly Lys Gly Ala Ser Asp Gly Ile 
145                 150                 155                 160 


Val Arg Val Trp Asp Val Lys Asn Ala Glu Leu Leu Asn Asn Gln Phe 
                165                 170                 175     


Gly Thr Met Pro Ser Leu Thr Leu Ala Cys Leu Thr Lys Gln Gly Gln 
            180                 185                 190         


Val Asp Leu Asn Asp Ala Val Gln Ala Leu Thr Asp Leu Gly Leu Ile 
        195                 200                 205             


Tyr Thr Ala Lys Tyr Pro Asn Ser Ser Asp Leu Asp Arg Leu Ser Gln 
    210                 215                 220                 


Ser His Pro Ile Leu Asn Met Ile Asp Thr Lys Lys Ser Ser Leu Asn 
225                 230                 235                 240 


Ile Ser Gly Tyr Asn Phe Ser Leu Gly Ala Ala Val Lys Ala Gly Ala 
                245                 250                 255     


Cys Met Leu Asp Gly Gly Asn Met Leu Glu Thr Ile Lys Val Ser Pro 
            260                 265                 270         


Gln Thr Met Asp Gly Ile Leu Lys Ser Ile Leu Lys Val Lys Lys Ser 
        275                 280                 285             


Leu Gly Met Phe Val Ser Asp Thr Pro Gly Glu Arg Asn Pro Tyr Glu 
    290                 295                 300                 


Asn Ile Leu Tyr Lys Ile Cys Leu Ser Gly Asp Gly Trp Pro Tyr Ile 
305                 310                 315                 320 


Ala Ser Arg Thr Ser Ile Val Gly Arg Ala Trp Glu Asn Thr Val Val 
                325                 330                 335     


Asp Leu Glu Gln Asp Asn Lys Pro Gln Lys Ile Gly Asn Gly Gly Ser 
            340                 345                 350         


Asn Lys Ser Leu Gln Ser Ala Gly Phe Ala Ala Gly Leu Thr Tyr Ser 
        355                 360                 365             


Gln Leu Met Thr Leu Lys Asp Phe Lys Cys Phe Asn Leu Ile Pro Asn 
    370                 375                 380                 


Ala Lys Thr Trp Met Asp Ile Glu Gly Arg Pro Glu Asp Pro Val Glu 
385                 390                 395                 400 


Ile Ala Leu Tyr Gln Pro Ser Ser Gly Cys Tyr Val His Phe Phe Arg 
                405                 410                 415     


Glu Pro Thr Asp Leu Lys Gln Phe Lys Gln Asp Ala Lys Tyr Ser His 
            420                 425                 430         


Gly Ile Asp Val Thr Asp Leu Phe Ala Ala Gln Pro Gly Leu Thr Ser 
        435                 440                 445             


Ala Val Ile Glu Ala Leu Pro Arg Asn Met Val Ile Thr Cys Gln Gly 
    450                 455                 460                 


Ser Glu Asp Ile Arg Lys Leu Leu Glu Ser Gln Gly Arg Arg Asp Ile 
465                 470                 475                 480 


Lys Leu Ile Asp Ile Thr Leu Ser Lys Ala Asp Ser Arg Lys Phe Glu 
                485                 490                 495     


Asn Ala Val Trp Asp Gln Phe Lys Asp Leu Cys His Met His Thr Gly 
            500                 505                 510         


Val Val Val Glu Lys Lys Lys Arg Gly Gly Lys Glu Glu Ile Thr Pro 
        515                 520                 525             


His Cys Ala Leu Met Asp Cys Ile Met Phe Asp Ala Ala Val Ser Gly 
    530                 535                 540                 


Gly Leu Asp Ala Lys Val Leu Arg Val Val Leu Pro Arg Asp Met Val 
545                 550                 555                 560 


Phe Arg Thr Ser Thr Pro Lys Val Val Leu 
                565                 570 


<210>  6
<211>  91
<212>  PRT
<213>  Lassa virus

<400>  6

Lys Ser Thr Gln Gln Lys Ser Val Leu Arg Val Gly Thr Leu Ser Ser 
1               5                   10                  15      


Asp Asp Leu Leu Ile Leu Ala Ala Asp Leu Glu Lys Leu Lys Ser Lys 
            20                  25                  30          


Val Thr Arg Thr Glu Arg Pro Leu Ser Ser Gly Val Tyr Met Gly Asn 
        35                  40                  45              


Leu Ser Ser Gln Gln Leu Asp Gln Arg Arg Ala Leu Leu Asn Met Ile 
    50                  55                  60                  


Gly Met Thr Gly Val Ser Gly Gly Gly Lys Gly Ala Ser Asp Gly Ile 
65                  70                  75                  80  


Val Arg Val Trp Asp Val Lys Asn Ala Glu Leu 
                85                  90      


<210>  7
<211>  91
<212>  PRT
<213>  Lassa virus

<400>  7

Ile Ser Gly Tyr Asn Phe Ser Leu Gly Ala Ala Val Lys Ala Gly Ala 
1               5                   10                  15      


Cys Met Leu Asp Gly Gly Asn Met Leu Glu Thr Ile Lys Val Ser Pro 
            20                  25                  30          


Gln Thr Met Asp Gly Ile Leu Lys Ser Ile Leu Lys Val Lys Lys Ser 
        35                  40                  45              


Leu Gly Met Phe Val Ser Asp Thr Pro Gly Glu Arg Asn Pro Tyr Glu 
    50                  55                  60                  


Asn Ile Leu Tyr Lys Ile Cys Leu Ser Gly Asp Gly Trp Pro Tyr Ile 
65                  70                  75                  80  


Ala Ser Arg Thr Ser Ile Val Gly Arg Ala Trp 
                85                  90      


<210>  8
<211>  274
<212>  DNA
<213>  Lassa virus

<400>  8
caaatccaca cagcagaaaa gtgtcttaag agttggaacc ttaagttcag acgatctact       60

aatcctggcc gctgatttag aaaaactgaa atcaaaagtc accagaacag aaaggccttt      120

gagttcagga gtttatatgg ggaatttgag ttcacaacag cttgatcaaa ggagagccct      180

tttgaacatg attggcatga ctggagtaag tggaggggga aagggtgcca gtgatggcat      240

tgtgagagtt tgggatgtca aaaatgcaga gtta                                  274


<210>  9
<211>  274
<212>  DNA
<213>  Lassa virus

<400>  9
catctctggt tacaatttca gtttgggtgc tgctgtcaaa gcaggggcct gcatgcttga       60

tggtggtaac atgttagaga ctattaaggt ttcacctcag accatggatg gtatcttgaa      120

gtcaatcttg aaagttaaga agagtctggg aatgtttgta tcagacacac cgggtgaaag      180

gaacccttat gagaacatcc tatacaagat ctgtctctca ggagacggat ggccctatat      240

tgcatcaagg acctcgattg tgggaagagc atgg                                  274


<210>  10
<211>  4123
<212>  DNA
<213>  Artificial

<220>
<223>  pMVALassaNP

<400>  10
gttggtggtc gccatggatg gtgttattgt atactgtcta aacgcgttag taaaacatgg       60

cgaggaaata aatcatataa aaaatgattt catgattaaa ccatgttgtg aaaaagtcaa      120

gaacgttcac attggcggac aatctaaaaa caatacagtg attgcagatt tgccatatat      180

ggataatgcg gtatccgatg tatgcaattc actgtataaa aagaatgtat caagaatatc      240

cagatttgct aatttgataa agatagatga cgatgacaag actcctactg gtgtatataa      300

ttattttaaa cctaaagatg ccattcctgt tattatatcc ataggaaagg atagagatgt      360

ttgtgaacta ttaatctcat ctgataaagc gtgtgcgtgt atagagttaa attcatataa      420

agtagccatt cttcccatgg atgtttcctt ttttaccaaa ggaaatgcat cattgattat      480

tctcctgttt gatttctcta tcgatgcggc acctctctta agaagtgtaa ccgataataa      540

tgttattata tctagacacc agcgtctaca tgacgagctt ccgagttcca attggttcaa      600

gttttacata agtataaagt ccgactattg ttctatatta tatatggttg ttgatggatc      660

tgtgatgcat gcaatagctg ataatagaac ttacgcaaat attagcaaaa atatattaga      720

caatactaca attaacgatg agtgtagatg ctgttatttt gaaccacaga ttaggattct      780

tgatagagat gagatgctca atggatcatc gtgtgatatg aacagacatt gtattatgat      840

gaatttacct gatgtaggcg aatttggatc tagtatgttg gggaaatatg aacctgacat      900

gattaagatt gctctttcgg tggctgggta ccaggcgcgc ctttcatttt gtttttttct      960

atgctataaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc     1020

gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat     1080

gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc     1140

tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac     1200

cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc     1260

accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc     1320

gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc     1380

ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag     1440

cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg     1500

cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc     1560

gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat     1620

cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg     1680

tacaagtaag agctccggcc cgctcgaggc cgctggtacc caacctaaaa attgaaaata     1740

aatacaaagg ttcttgaggg ttgtgttaaa ttgaaagcga gaaataatca taaataagcc     1800

cggtgccacc atgagtgctt ccaaggaagt gaggtcattc ttgtggactc aatccctaag     1860

aagggaacta tctggctact gttccaacat aaagttgcag gtagttaaag acgctcaagc     1920

tctccttcat ggtcttgact tctccgaggt cagtaatgtt cagagattga tgcgcaagca     1980

gaaaagagat gatggcgacc taaaacgact gagagatctc aatcaagcag tcaacaatct     2040

tgttgagctc aaatccacac agcagaaaag tgtcttaaga gttggaacct taagttcaga     2100

cgatctacta atcctggccg ctgatttaga aaaactgaaa tcaaaagtca ccagaacaga     2160

aaggcctttg agttcaggag tttatatggg gaatttgagt tcacaacagc ttgatcaaag     2220

gagagccctt ttgaacatga ttggcatgac tggagtaagt ggagggggaa agggtgccag     2280

tgatggcatt gtgagagttt gggatgtcaa aaatgcagag ttactcaaca atcagttcgg     2340

aacaatgcca agcctaactt tagcatgcct gaccaaacag gggcaagtgg acctgaatga     2400

tgccgttcaa gctttgacag atttagggct gatttacaca gccaaatacc ccaactcatc     2460

tgatctcgac agattgtctc agagtcatcc aattctgaat atgattgaca ctaagaaaag     2520

ttcactcaac atctctggtt acaatttcag tttgggtgct gctgtcaaag caggggcctg     2580

catgcttgat ggtggtaaca tgttagagac tattaaggtt tcacctcaga ccatggatgg     2640

tatcttgaag tcaatcttga aagttaagaa gagtctggga atgtttgtat cagacacacc     2700

gggtgaaagg aacccttatg agaacatcct atacaagatc tgtctctcag gagacggatg     2760

gccctatatt gcatcaagga cctcgattgt gggaagagca tgggaaaata ctgtggtgga     2820

ccttgagcaa gacaacaagc cccagaaaat tggaaatggg gggtccaaca agtcattaca     2880

gtctgcaggc tttgctgcag gattaactta ctctcagttg atgactctca aagatttcaa     2940

gtgcttcaac ttgattccca acgcaaaaac ctggatggat attgaaggaa gaccagaaga     3000

cccagttgag atagcccttt atcaaccgag ctcgggttgc tatgtacatt tctttaggga     3060

gccaacagat ttgaagcaat tcaaacaaga tgcaaagtat tcacatggta ttgatgtgac     3120

tgatttgttt gctgcccaac ctgggttaac cagtgcagtg atagaagccc ttcctcggaa     3180

catggtcatc acttgccaag gatcagagga tatcagaaaa ctccttgagt cacaagggag     3240

gagagacata aaactgattg acatcactct tagtaaagca gattcaagaa agtttgagaa     3300

tgctgtttgg gatcaattca aggatctatg tcacatgcac actggggtag ttgtggagaa     3360

aaagaagaga ggtggtaaag aggaaataac tcctcattgt gcactgatgg attgcattat     3420

gtttgatgca gcagtttcag gaggacttga tgcaaaagtc ctgagagctg tgctccctag     3480

agacatggtg ttcagaactt caacacctaa agtcgtcctg gatctagagg gcccgcggtt     3540

cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgtaac tcgacctgca     3600

gggaaagttt tataggtagt tgatagaaca aaatacataa ttttgtaaaa ataaatcact     3660

ttttatacta atatgacacg attaccaata cttttgttac taatatcatt agtatacgct     3720

acaccttttc ctcagacatc taaaaaaata ggtgatgatg caactttatc atgtaatcga     3780

aataatacaa atgactacgt tgttatgagt gcttggtata aggagcccaa ttccattatt     3840

cttttagctg ctaaaagcga cgtcttgtat tttgataatt ataccaagga taaaatatct     3900

tacgactctc catacgatga tctagttaca actatcacaa ttaaatcatt gactgctaga     3960

gatgccggta cttatgtatg tgcattcttt atgacatcgc ctacaaatga cactgataaa     4020

gtagattatg aagaatactc cacagagttg attgtaaata cagatagtga atcgactata     4080

gacataatac tatctggatc tacacattca ccggaaacta gtt                       4123


<210>  11
<211>  941
<212>  DNA
<213>  Artificial

<220>
<223>  DelIII Left flank

<400>  11
gttggtggtc gccatggatg gtgttattgt atactgtcta aacgcgttag taaaacatgg       60

cgaggaaata aatcatataa aaaatgattt catgattaaa ccatgttgtg aaaaagtcaa      120

gaacgttcac attggcggac aatctaaaaa caatacagtg attgcagatt tgccatatat      180

ggataatgcg gtatccgatg tatgcaattc actgtataaa aagaatgtat caagaatatc      240

cagatttgct aatttgataa agatagatga cgatgacaag actcctactg gtgtatataa      300

ttattttaaa cctaaagatg ccattcctgt tattatatcc ataggaaagg atagagatgt      360

ttgtgaacta ttaatctcat ctgataaagc gtgtgcgtgt atagagttaa attcatataa      420

agtagccatt cttcccatgg atgtttcctt ttttaccaaa ggaaatgcat cattgattat      480

tctcctgttt gatttctcta tcgatgcggc acctctctta agaagtgtaa ccgataataa      540

tgttattata tctagacacc agcgtctaca tgacgagctt ccgagttcca attggttcaa      600

gttttacata agtataaagt ccgactattg ttctatatta tatatggttg ttgatggatc      660

tgtgatgcat gcaatagctg ataatagaac ttacgcaaat attagcaaaa atatattaga      720

caatactaca attaacgatg agtgtagatg ctgttatttt gaaccacaga ttaggattct      780

tgatagagat gagatgctca atggatcatc gtgtgatatg aacagacatt gtattatgat      840

gaatttacct gatgtaggcg aatttggatc tagtatgttg gggaaatatg aacctgacat      900

gattaagatt gctctttcgg tggctgggta ccaggcgcgc c                          941


<210>  12
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  p11

<400>  12
tttcattttg tttttttcta tgctataa                                          28


<210>  13
<211>  720
<212>  DNA
<213>  Artificial

<220>
<223>  GFP

<400>  13
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa      720


<210>  14
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer

<400>  14
gagctccggc ccgctcgagg ccgctggtac ccaacct                                37


<210>  15
<211>  71
<212>  DNA
<213>  Artificial

<220>
<223>  MH5 promoter

<400>  15
aaaaattgaa aataaataca aaggttcttg agggttgtgt taaattgaaa gcgagaaata       60

atcataaata a                                                            71


<210>  16
<211>  13
<212>  DNA
<213>  Artificial

<220>
<223>  Kozak sequence

<400>  16
gcccggtgcc acc                                                          13


<210>  17
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Spacer

<400>  17
gatctagagg gcccgcggtt cgaa                                              24


<210>  18
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  V5 tag

<400>  18
ggtaagccta tccctaaccc tctcctcggt ctcgattcta cg                          42


<210>  19
<211>  534
<212>  DNA
<213>  Artificial

<220>
<223>  DelIII left flank

<400>  19
ctcgacctgc agggaaagtt ttataggtag ttgatagaac aaaatacata attttgtaaa       60

aataaatcac tttttatact aatatgacac gattaccaat acttttgtta ctaatatcat      120

tagtatacgc tacacctttt cctcagacat ctaaaaaaat aggtgatgat gcaactttat      180

catgtaatcg aaataataca aatgactacg ttgttatgag tgcttggtat aaggagccca      240

attccattat tcttttagct gctaaaagcg acgtcttgta ttttgataat tataccaagg      300

ataaaatatc ttacgactct ccatacgatg atctagttac aactatcaca attaaatcat      360

tgactgctag agatgccggt acttatgtat gtgcattctt tatgacatcg cctacaaatg      420

acactgataa agtagattat gaagaatact ccacagagtt gattgtaaat acagatagtg      480

aatcgactat agacataata ctatctggat ctacacattc accggaaact agtt            534


<210>  20
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  20
cggcacctct cttaagaagt                                                   20


<210>  21
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  21
gtgtagcgta tactaatgat attag                                             25


<210>  22
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  22
ggagtacaac tacaacagcc acaacg                                            26


<210>  23
<211>  604
<212>  PRT
<213>  Artificial

<220>
<223>  Expressed protein

<400>  23

Lys Arg Glu Ile Ile Ile Asn Lys Pro Gly Ala Thr Met Ser Ala Ser 
1               5                   10                  15      


Lys Glu Val Arg Ser Phe Leu Trp Thr Gln Ser Leu Arg Arg Glu Leu 
            20                  25                  30          


Ser Gly Tyr Cys Ser Asn Ile Lys Leu Gln Val Val Lys Asp Ala Gln 
        35                  40                  45              


Ala Leu Leu His Gly Leu Asp Phe Ser Glu Val Ser Asn Val Gln Arg 
    50                  55                  60                  


Leu Met Arg Lys Gln Lys Arg Asp Asp Gly Asp Leu Lys Arg Leu Arg 
65                  70                  75                  80  


Asp Leu Asn Gln Ala Val Asn Asn Leu Val Glu Leu Lys Ser Thr Gln 
                85                  90                  95      


Gln Lys Ser Val Leu Arg Val Gly Thr Leu Ser Ser Asp Asp Leu Leu 
            100                 105                 110         


Ile Leu Ala Ala Asp Leu Glu Lys Leu Lys Ser Lys Val Thr Arg Thr 
        115                 120                 125             


Glu Arg Pro Leu Ser Ser Gly Val Tyr Met Gly Asn Leu Ser Ser Gln 
    130                 135                 140                 


Gln Leu Asp Gln Arg Arg Ala Leu Leu Asn Met Ile Gly Met Thr Gly 
145                 150                 155                 160 


Val Ser Gly Gly Gly Lys Gly Ala Ser Asp Gly Ile Val Arg Val Trp 
                165                 170                 175     


Asp Val Lys Asn Ala Glu Leu Leu Asn Asn Gln Phe Gly Thr Met Pro 
            180                 185                 190         


Ser Leu Thr Leu Ala Cys Leu Thr Lys Gln Gly Gln Val Asp Leu Asn 
        195                 200                 205             


Asp Ala Val Gln Ala Leu Thr Asp Leu Gly Leu Ile Tyr Thr Ala Lys 
    210                 215                 220                 


Tyr Pro Asn Ser Ser Asp Leu Asp Arg Leu Ser Gln Ser His Pro Ile 
225                 230                 235                 240 


Leu Asn Met Ile Asp Thr Lys Lys Ser Ser Leu Asn Ile Ser Gly Tyr 
                245                 250                 255     


Asn Phe Ser Leu Gly Ala Ala Val Lys Ala Gly Ala Cys Met Leu Asp 
            260                 265                 270         


Gly Gly Asn Met Leu Glu Thr Ile Lys Val Ser Pro Gln Thr Met Asp 
        275                 280                 285             


Gly Ile Leu Lys Ser Ile Leu Lys Val Lys Lys Ser Leu Gly Met Phe 
    290                 295                 300                 


Val Ser Asp Thr Pro Gly Glu Arg Asn Pro Tyr Glu Asn Ile Leu Tyr 
305                 310                 315                 320 


Lys Ile Cys Leu Ser Gly Asp Gly Trp Pro Tyr Ile Ala Ser Arg Thr 
                325                 330                 335     


Ser Ile Val Gly Arg Ala Trp Glu Asn Thr Val Val Asp Leu Glu Gln 
            340                 345                 350         


Asp Asn Lys Pro Gln Lys Ile Gly Asn Gly Gly Ser Asn Lys Ser Leu 
        355                 360                 365             


Gln Ser Ala Gly Phe Ala Ala Gly Leu Thr Tyr Ser Gln Leu Met Thr 
    370                 375                 380                 


Leu Lys Asp Phe Lys Cys Phe Asn Leu Ile Pro Asn Ala Lys Thr Trp 
385                 390                 395                 400 


Met Asp Ile Glu Gly Arg Pro Glu Asp Pro Val Glu Ile Ala Leu Tyr 
                405                 410                 415     


Gln Pro Ser Ser Gly Cys Tyr Val His Phe Phe Arg Glu Pro Thr Asp 
            420                 425                 430         


Leu Lys Gln Phe Lys Gln Asp Ala Lys Tyr Ser His Gly Ile Asp Val 
        435                 440                 445             


Thr Asp Leu Phe Ala Ala Gln Pro Gly Leu Thr Ser Ala Val Ile Glu 
    450                 455                 460                 


Ala Leu Pro Arg Asn Met Val Ile Thr Cys Gln Gly Ser Glu Asp Ile 
465                 470                 475                 480 


Arg Lys Leu Leu Glu Ser Gln Gly Arg Arg Asp Ile Lys Leu Ile Asp 
                485                 490                 495     


Ile Thr Leu Ser Lys Ala Asp Ser Arg Lys Phe Glu Asn Ala Val Trp 
            500                 505                 510         


Asp Gln Phe Lys Asp Leu Cys His Met His Thr Gly Val Val Val Glu 
        515                 520                 525             


Lys Lys Lys Arg Gly Gly Lys Glu Glu Ile Thr Pro His Cys Ala Leu 
    530                 535                 540                 


Met Asp Cys Ile Met Phe Asp Ala Ala Val Ser Gly Gly Leu Asp Ala 
545                 550                 555                 560 


Lys Val Leu Arg Ala Val Leu Pro Arg Asp Met Val Phe Arg Thr Ser 
                565                 570                 575     


Thr Pro Lys Val Val Leu Asp Leu Glu Gly Pro Arg Phe Glu Gly Lys 
            580                 585                 590         


Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
        595                 600                 


<210>  24
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Translated 3' terminus of the MH5 promoter

<400>  24

Lys Arg Glu Ile Ile Ile Asn 
1               5           


<210>  25
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Translated 3' terminus of the MH5 promoter and Kozak sequence

<400>  25

Lys Pro Gly Ala Thr 
1               5   


<210>  26
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  V5 tag

<400>  26

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  27
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Translated second spacer

<400>  27

Asp Leu Glu Gly Pro Arg Phe Glu 
1               5               


