                         SEQUENCE LISTING

<110>  OREGON STATE UNIVERSITY
 
<120>  IMMUNOGENIC COMPOSITIONS COMPRISING MYCOBACTERIUM BOVIS SURFACE 
       PROTEINS AND USES THEREOF

<130>  245-100087-02

<150>  US 62/620,047
<151>  2018-01-22

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  1095
<212>  DNA
<213>  Mycobacterium bovis

<400>  1
gtgcaaggag ccgttgctgg tctggtgttt ctggccgtcc tggtgatttt cgccatcatc       60

gtggtggcca agtcggtggc gctgatcccg caggcggagg ccgcggtgat cgagcggctg      120

ggtcgctata gtcgtacggt cagtgggcag ttgacgctgt tggtgccgtt catcgaccgc      180

gtccgggctc gggtggacct gcgcgagcgg gtggtgtcgt ttccgccgca accggtgatc      240

accgaggaca acttgacgct gaacatcgac accgtcgtct acttccaggt gaccgttccg      300

caggcggcgg tgtacgagat cagcaattac atcgtcgggg tcgaacagct caccaccacc      360

accaccctgc gcaacgttgt cggcgggatg acgctggagc agacgttgac ctcgcgtgac      420

cagatcaacg cccagctgcg cggcgttctc gatgaggcga ccggccgctg gggtctgcgg      480

gtggcgcggg tggagctgcg cagcatcgat ccgccgccgt cgattcaggc gtcgatggaa      540

aagcagatga aggccgaccg ggagaagcga gcgatgattc tgaccgccga aggtacccgg      600

gaggcggcga taaaacaggc cgaggggcaa aagcaggcgc agatcctggc cgccgagggc      660

gccaagcagg ccgcgatctt ggctgctgag gccgatcggc agtctcggat gctgcgcgct      720

cagggtgagc gcgccgcggc ctacctgcag gcgcaagggc aggccaaggc catcgagaag      780

acgttcgccg cgatcaaggc tggccggccc accccggaga tgctggccta ccaatacctg      840

cagacgctgc cggagatggc gcgtggggac gccaacaagg tatgggtggt gcccagcgac      900

ttcaacgccg cactgcaggg ttcaccaggc tgctgggcaa gccgggtgag gacggggtgt      960

tccggttcga gccgtccccg gtcgaagacc agcccaagca cgcggccgac ggtgacgacg     1020

ccgaggtcgc cggctggttc tccaccgata ccgacccgtc gatcgctcgg gcggtggcta     1080

cagccgaggc gatag                                                      1095


<210>  2
<211>  381
<212>  PRT
<213>  Mycobacterium bovis

<400>  2

Met Gln Gly Ala Val Ala Gly Leu Val Phe Leu Ala Val Leu Val Ile 
1               5                   10                  15      


Phe Ala Ile Ile Val Val Ala Lys Ser Val Ala Leu Ile Pro Gln Ala 
            20                  25                  30          


Glu Ala Ala Val Ile Glu Arg Leu Gly Arg Tyr Ser Arg Thr Val Ser 
        35                  40                  45              


Gly Gln Leu Thr Leu Leu Val Pro Phe Ile Asp Arg Val Arg Ala Arg 
    50                  55                  60                  


Val Asp Leu Arg Glu Arg Val Val Ser Phe Pro Pro Gln Pro Val Ile 
65                  70                  75                  80  


Thr Glu Asp Asn Leu Thr Leu Asn Ile Asp Thr Val Val Tyr Phe Gln 
                85                  90                  95      


Val Thr Val Pro Gln Ala Ala Val Tyr Glu Ile Ser Asn Tyr Ile Val 
            100                 105                 110         


Gly Val Glu Gln Leu Thr Thr Thr Thr Leu Arg Asn Val Val Gly Gly 
        115                 120                 125             


Met Thr Leu Glu Gln Thr Leu Thr Ser Arg Asp Gln Ile Asn Ala Gln 
    130                 135                 140                 


Leu Arg Gly Val Leu Asp Glu Ala Thr Gly Arg Trp Gly Leu Arg Val 
145                 150                 155                 160 


Ala Arg Val Glu Leu Arg Ser Ile Asp Pro Pro Pro Ser Ile Gln Ala 
                165                 170                 175     


Ser Met Glu Lys Gln Met Lys Ala Asp Arg Glu Lys Arg Ala Met Ile 
            180                 185                 190         


Leu Thr Ala Glu Gly Thr Arg Glu Ala Ala Ile Lys Gln Ala Glu Gly 
        195                 200                 205             


Gln Lys Gln Ala Gln Ile Leu Ala Ala Glu Gly Ala Lys Gln Ala Ala 
    210                 215                 220                 


Ile Leu Ala Ala Glu Ala Asp Arg Gln Ser Arg Met Leu Arg Ala Gln 
225                 230                 235                 240 


Gly Glu Arg Ala Ala Ala Tyr Leu Gln Ala Gln Gly Gln Ala Lys Ala 
                245                 250                 255     


Ile Glu Lys Thr Phe Ala Ala Ile Lys Ala Gly Arg Pro Thr Pro Glu 
            260                 265                 270         


Met Leu Ala Tyr Gln Tyr Leu Gln Thr Leu Pro Glu Met Ala Arg Gly 
        275                 280                 285             


Asp Ala Asn Lys Val Trp Val Val Pro Ser Asp Phe Asn Ala Ala Leu 
    290                 295                 300                 


Gln Gly Phe Thr Arg Leu Leu Gly Lys Pro Gly Glu Asp Gly Val Phe 
305                 310                 315                 320 


Arg Phe Glu Pro Ser Pro Val Glu Asp Gln Pro Lys His Ala Ala Asp 
                325                 330                 335     


Gly Asp Asp Ala Glu Val Ala Gly Trp Phe Ser Thr Asp Thr Asp Pro 
            340                 345                 350         


Ser Ile Ala Arg Ala Val Ala Thr Ala Glu Ala Ile Ala Arg Lys Pro 
        355                 360                 365             


Val Glu Gly Ser Leu Gly Thr Pro Pro Arg Leu Thr Gln 
    370                 375                 380     


<210>  3
<211>  480
<212>  DNA
<213>  Mycobacterium bovis

<400>  3
gtggacacaa ctgtcgctac catgatcagc aaatacatac agataaccgt ttgctcttgg       60

agcccggtgg aggtcacatc gatgagcacg acgttcgctg cccgcctgaa ccgcctgttc      120

gacacggttt atccgcccgg acgcgggcca catacctccg cggaggtgat cgcggcgctc      180

aaggcagagg gcatcacgat gtcggctccc tacctatcac agctacgctc aggaaaccgt      240

acgaacccat cgggggcgac catggccgcc ctggccaact tcttccgcat caaggcggcc      300

tacttcaccg acgacgagta ctacgaaaag ctcgacaagg aattgcagtg gctgtgcacg      360

atgcgcgacg acggcgtgcg ccggatcgcg cagcgggccc acgggttgcc ctccgcggcg      420

cagcagaagg tgttggaccg gatcgacgag ctgcggcgtg ccgaagggat cgacgcttag      480


<210>  4
<211>  159
<212>  PRT
<213>  Mycobacterium bovis

<400>  4

Met Asp Thr Thr Val Ala Thr Met Ile Ser Lys Tyr Ile Gln Ile Thr 
1               5                   10                  15      


Val Cys Ser Trp Ser Pro Val Glu Val Thr Ser Met Ser Thr Thr Phe 
            20                  25                  30          


Ala Ala Arg Leu Asn Arg Leu Phe Asp Thr Val Tyr Pro Pro Gly Arg 
        35                  40                  45              


Gly Pro His Thr Ser Ala Glu Val Ile Ala Ala Leu Lys Ala Glu Gly 
    50                  55                  60                  


Ile Thr Met Ser Ala Pro Tyr Leu Ser Gln Leu Arg Ser Gly Asn Arg 
65                  70                  75                  80  


Thr Asn Pro Ser Gly Ala Thr Met Ala Ala Leu Ala Asn Phe Phe Arg 
                85                  90                  95      


Ile Lys Ala Ala Tyr Phe Thr Asp Asp Glu Tyr Tyr Glu Lys Leu Asp 
            100                 105                 110         


Lys Glu Leu Gln Trp Leu Cys Thr Met Arg Asp Asp Gly Val Arg Arg 
        115                 120                 125             


Ile Ala Gln Arg Ala His Gly Leu Pro Ser Ala Ala Gln Gln Lys Val 
    130                 135                 140                 


Leu Asp Arg Ile Asp Glu Leu Arg Arg Ala Glu Gly Ile Asp Ala 
145                 150                 155                 


<210>  5
<211>  666
<212>  DNA
<213>  Mycobacterium bovis

<400>  5
atgcggacgg tctatcacca gcggctaacc gagttggccg gacgattggg agagatgtgc       60

agcctggccg ggatagcgat gaaacgcgca acgcaggctc tgctcgaggc cgacattggc      120

gccgctgaac aagtaatccg tgaccatgag cggatcgtgg cgatgcgagc ccaagtcgaa      180

aaggaagcgt tcgcgctgct ggcgttgcaa catccggtgt ccggcgagct gcgggaaatc      240

ttcagtgcgg tgcagatcat cgccgacacc gagcgcatgg gtgcgttggc tgtgcatatt      300

gccaagatca cccgacgcga gtatccgaac caggtgcttc ctgaggaagt tcgcaactgc      360

ttcgccgaca tggcgaaggt ggcaatcgcg ttgggtgaca gtgcaagaca agtgctggtg      420

aaccgtgacc cgcaggaagc cgcgcaactg cacgatcgtg acgacgcgat ggatgacctg      480

cataggcatt tgctgagcgt gctgatagat cgagaatggc ggcacggcgt tcgcgtcggt      540

gtggaaacgg cgttgctggg tcgtttcttt gagcgcttcg ccgaccacgc tgtggaagtg      600

ggccgccgcg tcatcttcat ggtcaccggg gtgctaccga ccgaggacga gatttccact      660

tactga                                                                 666


<210>  6
<211>  221
<212>  PRT
<213>  Mycobacterium bovis

<400>  6

Met Arg Thr Val Tyr His Gln Arg Leu Thr Glu Leu Ala Gly Arg Leu 
1               5                   10                  15      


Gly Glu Met Cys Ser Leu Ala Gly Ile Ala Met Lys Arg Ala Thr Gln 
            20                  25                  30          


Ala Leu Leu Glu Ala Asp Ile Gly Ala Ala Glu Gln Val Ile Arg Asp 
        35                  40                  45              


His Glu Arg Ile Val Ala Met Arg Ala Gln Val Glu Lys Glu Ala Phe 
    50                  55                  60                  


Ala Leu Leu Ala Leu Gln His Pro Val Ser Gly Glu Leu Arg Glu Ile 
65                  70                  75                  80  


Phe Ser Ala Val Gln Ile Ile Ala Asp Thr Glu Arg Met Gly Ala Leu 
                85                  90                  95      


Ala Val His Ile Ala Lys Ile Thr Arg Arg Glu Tyr Pro Asn Gln Val 
            100                 105                 110         


Leu Pro Glu Glu Val Arg Asn Cys Phe Ala Asp Met Ala Lys Val Ala 
        115                 120                 125             


Ile Ala Leu Gly Asp Ser Ala Arg Gln Val Leu Val Asn Arg Asp Pro 
    130                 135                 140                 


Gln Glu Ala Ala Gln Leu His Asp Arg Asp Asp Ala Met Asp Asp Leu 
145                 150                 155                 160 


His Arg His Leu Leu Ser Val Leu Ile Asp Arg Glu Trp Arg His Gly 
                165                 170                 175     


Val Arg Val Gly Val Glu Thr Ala Leu Leu Gly Arg Phe Phe Glu Arg 
            180                 185                 190         


Phe Ala Asp His Ala Val Glu Val Gly Arg Arg Val Ile Phe Met Val 
        195                 200                 205             


Thr Gly Val Leu Pro Thr Glu Asp Glu Ile Ser Thr Tyr 
    210                 215                 220     


<210>  7
<211>  584
<212>  DNA
<213>  Mycobacterium bovis

<400>  7
atggctgaaa actcgaacat tgatgacatc aaggctccgt tgcttgccgc gcttggagcg       60

gccgacctgg ccttggccac tgtcaacgag ttgatcacga acctgcgtga gcgtgcggag      120

gagactcgta cggacacccg cagccgggtc gaggagagcc gtgctcgcct gaccaagctg      180

caggaagatc tgcccgagca gctcaccgag ctgcgtgaga agttcaccgc cgaggagctg      240

cgtaaggccg ccgagggcta cctcgaggcc gcgactagcc ggtacaacga gctggtcgag      300

cgcggtgagg ccgctctaga gcggctgcgc agccagcaga gcttcgagga agtgtcggcg      360

cgcgccgaag gctacgtgga ccaggcggtg gagttgaccc aggaggcgtt gggtacggtc      420

gcatcgcaga cccgcgcggt cggtgagcgt gccgccaagc tggtcggcat cgagctgcct      480

aagaaggctg ctccggccaa gaaggccgct ccggccaaga aggccgctcc ggccaagaag      540

gcggcggcca agaaggcgcc cgcgaagaag gcggcggcca agaa                       584


<210>  8
<211>  194
<212>  PRT
<213>  Mycobacterium bovis

<400>  8

Met Ala Glu Asn Ser Asn Ile Asp Asp Ile Lys Ala Pro Leu Leu Ala 
1               5                   10                  15      


Ala Leu Gly Ala Ala Asp Leu Ala Leu Ala Thr Val Asn Glu Leu Ile 
            20                  25                  30          


Thr Asn Leu Arg Glu Arg Ala Glu Glu Thr Arg Thr Asp Thr Arg Ser 
        35                  40                  45              


Arg Val Glu Glu Ser Arg Ala Arg Leu Thr Lys Leu Gln Glu Asp Leu 
    50                  55                  60                  


Pro Glu Gln Leu Thr Glu Leu Arg Glu Lys Phe Thr Ala Glu Glu Leu 
65                  70                  75                  80  


Arg Lys Ala Ala Glu Gly Tyr Leu Glu Ala Ala Thr Ser Arg Tyr Asn 
                85                  90                  95      


Glu Leu Val Glu Arg Gly Glu Ala Ala Leu Glu Arg Leu Arg Ser Gln 
            100                 105                 110         


Gln Ser Phe Glu Glu Val Ser Ala Arg Ala Glu Gly Tyr Val Asp Gln 
        115                 120                 125             


Ala Val Glu Leu Thr Gln Glu Ala Leu Gly Thr Val Ala Ser Gln Thr 
    130                 135                 140                 


Arg Ala Val Gly Glu Arg Ala Ala Lys Leu Val Gly Ile Glu Leu Pro 
145                 150                 155                 160 


Lys Lys Ala Ala Pro Ala Lys Lys Ala Ala Pro Ala Lys Lys Ala Ala 
                165                 170                 175     


Pro Ala Lys Lys Ala Ala Ala Lys Lys Ala Pro Ala Lys Lys Ala Ala 
            180                 185                 190         


Ala Lys 
        


<210>  9
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  9
tttttgaatt ccatcatcat catcatcatc aaggagccgt tgct                        44


<210>  10
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  10
tttttgtcga cctattgagt caacctgggg gg                                     32


<210>  11
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  11
tttttgaatt ccatcatcat catcatcatg acacaactgt c                           41


<210>  12
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  12
tttttgtcga cctaagcgtc gatccc                                            26


<210>  13
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  13
tttttgaatt ccatcatcat catcatcatc ggacggtcta t                           41


<210>  14
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  14
tttttgtcga ctcagtaagt ggaaatctcg tcct                                   34


<210>  15
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  15
tttttgaatt ccatcatcat catcatcatg ctgaaaactc gaac                        44


<210>  16
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  16
tttttgtcga cctacttctg ggtgaccttc tt                                     32


<210>  17
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  17
tttttgtcga ccaaggagcc gttgct                                            26


<210>  18
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  18
tttttgaatt cctattgagt caacctgggg gg                                     32


<210>  19
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  19
tttttgtcga cgacacaact gtcgct                                            26


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  20
tttttgaatt cctaagcgtc gatccc                                            26


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  21
tttttgtcga ccggacggtc tat                                               23


<210>  22
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  22
ttttttgaat tccagtaagt ggaaatctcg tcct                                   34


<210>  23
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  23
tttttgtcga cgctgaaaac tcgaac                                            26


<210>  24
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide primer

<400>  24
tttttgaatt cctacttctg ggtgaccttc tt                                     32


