                         SEQUENCE LISTING

<110>  Vertex Pharmaceuticals Incorporated
       MAHAJAN, Sudipta
       WEINBERG, Marc Saul
       D'ASTOLFO, Diego Sebastian
 
<120>  DNA-PK INHIBITORS

<130>  14390-686

<150>  US 62/618,598
<151>  2018-01-17

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 target site

<400>  1
accccacagt ggggccacta                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NAV1.7 target

<400>  2
ggctgagcgt ccatcaacca                                                   20


<210>  3
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 sgRNA


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  2'-O-methyl 3'-phosphorothioate

<220>
<221>  misc_feature
<222>  (97)..(99)
<223>  2'-O-methyl 3'-phosphorothioate

<400>  3
accccacagu ggggccacua guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  4
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  NAV1.7 sgRNA


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  2'-O-methyl 3'-phosphorothioate

<220>
<221>  misc_feature
<222>  (97)..(99)
<223>  2'-O-methyl 3'-phosphorothioate

<400>  4
ggcugagcgu ccaucaacca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  5
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 PAM


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  2'-O-methyl 3'-phosphorothioate

<220>
<221>  misc_feature
<222>  (87)..(89)
<223>  2'-O-methyl 3'-phosphorothioate

<400>  5
gggtactttt atctgtcccc tccaccccac agtggggcca gaattctcag ctagggacag       60

gattggtgac agaaaagccc catccttagg                                        90


<210>  6
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 Non-PAM


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  2'-O-methyl 3'-phosphorothioate

<220>
<221>  misc_feature
<222>  (87)..(89)
<223>  2'-O-methyl 3'-phosphorothioate

<400>  6
cctaaggatg gggcttttct gtcaccaatc ctgtccctag ctgagaattc tggccccact       60

gtggggtgga ggggacagat aaaagtaccc                                        90


<210>  7
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NAV1.7 PAM


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  2'-O-methyl 3'-phosphorothioate

<220>
<221>  misc_feature
<222>  (87)..(89)
<223>  2'-O-methyl 3'-phosphorothioate

<400>  7
agctgtccat tggggagcat gagggctgag cgtccatcaa ctgagaattc ccagggagac       60

cacaccgttg cagtccacag cactgtgcat                                        90


<210>  8
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NAV1.7 Non-PAM


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  2'-O-methyl 3'-phosphorothioate

<220>
<221>  misc_feature
<222>  (87)..(89)
<223>  2'-O-methyl 3'-phosphorothioate

<400>  8
atgcacagtg ctgtggactg caacggtgtg gtctccctgg gaattctcag ttgatggacg       60

ctcagccctc atgctcccca atggacagct                                        90


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 FW

<400>  9
ggacaacccc aaagtacccc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AAVS1 RV

<400>  10
aggatcagtg aaacgcacca                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NAV1.7 FW

<400>  11
gccagtgggt tcagtggtat                                                   20


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NAV1.7 RV

<400>  12
tcagcattat ccttgcattt tctgt                                             25


