                         SEQUENCE LISTING

<110>  The Board of Trustees of the Leland Stanford Junior 
       University
 
<120>  ENGINEERED PROMISCUOUS BIOTIN LIGASES FOR EFFICIENT PROXIMITY 
       LABELING

<130>  S17-352

<150>  62/618,344
<151>  2018-01-17

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer


<220>
<221>  source
<222>  (1)..(56)
<223>  sequence is synthesized

<400>  1
ctagtggtgg aggaggctct ggtggaggcg gtagcggagg cggagggtcg gctagc           56


<210>  2
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer


<220>
<221>  source
<222>  (1)..(56)
<223>  sequence is synthesized

<400>  2
tatcagatct cgagctatta caagtcctct tcagaaataa gcttttgttc ggatcc           56


<210>  3
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer


<220>
<221>  source
<222>  (1)..(37)
<223>  sequence is synthesized

<400>  3
caaggtctgc aggctagtgg tggaggaggc tctggtg                                37


<210>  4
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer


<220>
<221>  source
<222>  (1)..(38)
<223>  sequence is synthesized

<400>  4
ctacactgtt gttatcagat ctcgagctat tacaagtc                               38


<210>  5
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V5-BirA-mut _F primer


<220>
<221>  source
<222>  (1)..(38)
<223>  sequence is synthesized

<400>  5
ccgcggccgc ccccttcacc atgggcaagc ccatcccc                               38


<210>  6
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V5-BirA-mut _R primer


<220>
<221>  source
<222>  (1)..(41)
<223>  sequence is synthesized

<400>  6
gggtcggcgc gcccaccctt ctattagtcc agggtcaggc g                           41


<210>  7
<211>  321
<212>  PRT
<213>  Escherichia coli

<300>
<308>  NCBI/WP_023308552
<309>  2015-05-29
<313>  (1)..(321)

<400>  7

Met Lys Asp Asn Thr Val Pro Leu Lys Leu Ile Ala Leu Leu Ala Asn 
1               5                   10                  15      


Gly Glu Phe His Ser Gly Glu Gln Leu Gly Glu Thr Leu Gly Met Ser 
            20                  25                  30          


Arg Ala Ala Ile Asn Lys His Ile Gln Thr Leu Arg Asp Trp Gly Val 
        35                  40                  45              


Asp Val Phe Thr Val Pro Gly Lys Gly Tyr Ser Leu Pro Glu Pro Ile 
    50                  55                  60                  


Gln Leu Leu Asn Ala Lys Gln Ile Leu Gly Gln Leu Asp Gly Gly Ser 
65                  70                  75                  80  


Val Ala Val Leu Pro Val Ile Asp Ser Thr Asn Gln Tyr Leu Leu Asp 
                85                  90                  95      


Arg Ile Gly Glu Leu Lys Ser Gly Asp Ala Cys Val Ala Glu Tyr Gln 
            100                 105                 110         


Gln Ala Gly Arg Gly Arg Arg Gly Arg Lys Trp Phe Ser Pro Phe Gly 
        115                 120                 125             


Ala Asn Leu Tyr Leu Ser Met Phe Trp Arg Leu Glu Gln Gly Pro Ala 
    130                 135                 140                 


Ala Ala Ile Gly Leu Ser Leu Val Ile Gly Ile Val Met Ala Glu Val 
145                 150                 155                 160 


Leu Arg Lys Leu Gly Ala Asp Lys Val Arg Val Lys Trp Pro Asn Asp 
                165                 170                 175     


Leu Tyr Leu Gln Asp Arg Lys Leu Ala Gly Ile Leu Val Glu Leu Thr 
            180                 185                 190         


Gly Lys Thr Gly Asp Ala Ala Gln Ile Val Ile Gly Ala Gly Ile Asn 
        195                 200                 205             


Met Ala Met Arg Arg Val Glu Glu Ser Val Val Asn Gln Gly Trp Ile 
    210                 215                 220                 


Thr Leu Gln Glu Ala Gly Ile Asn Leu Asp Arg Asn Thr Leu Ala Ala 
225                 230                 235                 240 


Met Leu Ile Arg Glu Leu Arg Ala Ala Leu Glu Leu Phe Glu Gln Glu 
                245                 250                 255     


Gly Leu Ala Pro Tyr Leu Ser Arg Trp Glu Lys Leu Asp Asn Phe Ile 
            260                 265                 270         


Asn Arg Pro Val Lys Leu Ile Ile Gly Asp Lys Glu Ile Phe Gly Ile 
        275                 280                 285             


Ser Arg Gly Ile Asp Lys Gln Gly Ala Leu Leu Leu Glu Gln Asp Gly 
    290                 295                 300                 


Ile Ile Lys Pro Trp Met Gly Gly Glu Ile Ser Leu Arg Ser Ala Glu 
305                 310                 315                 320 


Lys 
    


<210>  8
<211>  957
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BioID


<220>
<221>  source
<222>  (1)..(957)
<223>  sequence produced by mutagenesis of E. coli BirA

<400>  8
aaggataaca ccgtgcccct gaaactgatt gctctgctgg ctaatggcga gttccatagt       60

ggcgaacagc tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag      120

actctgcgcg actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc      180

gagcctatcc agctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg      240

gcagtcctgc ctgtgatcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg      300

aagagtgggg atgcttgcat tgcagaatat cagcaggcag gaagaggagg gagagggagg      360

aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg cctggagcag      420

ggaccagcag caatcggcct gagcctggtc atcggaattg tcatggcaga agtgctgcga      480

aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga      540

aagctggcag gcatcctggt ggagctgacc ggaaaaacag gcgatgctgc acagatcgtc      600

attggcgccg ggattaacat ggctatgagg cgcgtggagg aaagcgtggt caatcagggc      660

tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc cgctatgctg      720

atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg      780

tcacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac      840

aaagagattt tcgggattag ccggggaatt gataaacagg gagccctgct gctggaacag      900

gacggaatta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaag         957


<210>  9
<211>  319
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BioID

<400>  9

Lys Asp Asn Thr Val Pro Leu Lys Leu Ile Ala Leu Leu Ala Asn Gly 
1               5                   10                  15      


Glu Phe His Ser Gly Glu Gln Leu Gly Glu Thr Leu Gly Met Ser Arg 
            20                  25                  30          


Ala Ala Ile Asn Lys His Ile Gln Thr Leu Arg Asp Trp Gly Val Asp 
        35                  40                  45              


Val Phe Thr Val Pro Gly Lys Gly Tyr Ser Leu Pro Glu Pro Ile Gln 
    50                  55                  60                  


Leu Leu Asn Ala Lys Gln Ile Leu Gly Gln Leu Asp Gly Gly Ser Val 
65                  70                  75                  80  


Ala Val Leu Pro Val Ile Asp Ser Thr Asn Gln Tyr Leu Leu Asp Arg 
                85                  90                  95      


Ile Gly Glu Leu Lys Ser Gly Asp Ala Cys Ile Ala Glu Tyr Gln Gln 
            100                 105                 110         


Ala Gly Arg Gly Gly Arg Gly Arg Lys Trp Phe Ser Pro Phe Gly Ala 
        115                 120                 125             


Asn Leu Tyr Leu Ser Met Phe Trp Arg Leu Glu Gln Gly Pro Ala Ala 
    130                 135                 140                 


Ile Gly Leu Ser Leu Val Ile Gly Ile Val Met Ala Glu Val Leu Arg 
145                 150                 155                 160 


Lys Leu Gly Ala Asp Lys Val Arg Val Lys Trp Pro Asn Asp Leu Tyr 
                165                 170                 175     


Leu Gln Asp Arg Lys Leu Ala Gly Ile Leu Val Glu Leu Thr Gly Lys 
            180                 185                 190         


Thr Gly Asp Ala Ala Gln Ile Val Ile Gly Ala Gly Ile Asn Met Ala 
        195                 200                 205             


Met Arg Arg Val Glu Glu Ser Val Val Asn Gln Gly Trp Ile Thr Leu 
    210                 215                 220                 


Gln Glu Ala Gly Ile Asn Leu Asp Arg Asn Thr Leu Ala Ala Met Leu 
225                 230                 235                 240 


Ile Arg Glu Leu Arg Ala Ala Leu Glu Leu Phe Glu Gln Glu Gly Leu 
                245                 250                 255     


Ala Pro Tyr Leu Ser Arg Trp Glu Lys Leu Asp Asn Phe Ile Asn Arg 
            260                 265                 270         


Pro Val Lys Leu Ile Ile Gly Asp Lys Glu Ile Phe Gly Ile Ser Arg 
        275                 280                 285             


Gly Ile Asp Lys Gln Gly Ala Leu Leu Leu Glu Gln Asp Gly Ile Ile 
    290                 295                 300                 


Lys Pro Trp Met Gly Gly Glu Ile Ser Leu Arg Ser Ala Glu Lys 
305                 310                 315                 


<210>  10
<211>  771
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MiniTurbo


<220>
<221>  source
<222>  (1)..(771)
<223>  sequence produced by mutagenesis of BioID

<400>  10
atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc       60

ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt      120

ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg      180

ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca      240

gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg      300

ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg      360

gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc      420

gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc      480

acactgcagg aagcagggat taacctggac aggaatactc tggccgctat gctgatccga      540

gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg      600

tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag      660

attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga      720

gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa g               771


<210>  11
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MiniTurbo

<400>  11

Ile Pro Leu Leu Asn Ala Lys Gln Ile Leu Gly Gln Leu Asp Gly Gly 
1               5                   10                  15      


Ser Val Ala Val Leu Pro Val Val Asp Ser Thr Asn Gln Tyr Leu Leu 
            20                  25                  30          


Asp Arg Ile Gly Glu Leu Lys Ser Gly Asp Ala Cys Ile Ala Glu Tyr 
        35                  40                  45              


Gln Gln Ala Gly Arg Gly Ser Arg Gly Arg Lys Trp Phe Ser Pro Phe 
    50                  55                  60                  


Gly Ala Asn Leu Tyr Leu Ser Met Phe Trp Arg Leu Lys Arg Gly Pro 
65                  70                  75                  80  


Ala Ala Ile Gly Leu Gly Pro Val Ile Gly Ile Val Met Ala Glu Ala 
                85                  90                  95      


Leu Arg Lys Leu Gly Ala Asp Lys Val Arg Val Lys Trp Pro Asn Asp 
            100                 105                 110         


Leu Tyr Leu Gln Asp Arg Lys Leu Ala Gly Ile Leu Val Glu Leu Ala 
        115                 120                 125             


Gly Ile Thr Gly Asp Ala Ala Gln Ile Val Ile Gly Ala Gly Ile Asn 
    130                 135                 140                 


Val Ala Met Arg Arg Val Glu Glu Ser Val Val Asn Gln Gly Trp Ile 
145                 150                 155                 160 


Thr Leu Gln Glu Ala Gly Ile Asn Leu Asp Arg Asn Thr Leu Ala Ala 
                165                 170                 175     


Met Leu Ile Arg Glu Leu Arg Ala Ala Leu Glu Leu Phe Glu Gln Glu 
            180                 185                 190         


Gly Leu Ala Pro Tyr Leu Ser Arg Trp Glu Lys Leu Asp Asn Phe Ile 
        195                 200                 205             


Asn Arg Pro Val Lys Leu Ile Ile Gly Asp Lys Glu Ile Phe Gly Ile 
    210                 215                 220                 


Ser Arg Gly Ile Asp Lys Gln Gly Ala Leu Leu Leu Glu Gln Asp Gly 
225                 230                 235                 240 


Val Ile Lys Pro Trp Met Gly Gly Glu Ile Ser Leu Arg Ser Ala Glu 
                245                 250                 255     


Lys 
    


<210>  12
<211>  957
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TurboID


<220>
<221>  source
<222>  (1)..(957)
<223>  sequence produced by mutagenesis of BioID

<400>  12
aaagacaata ctgtgcctct gaagctgatc gctctcctgg ctaatggcga gttccatagt       60

ggcgaacagc tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag      120

actctgcgcg actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc      180

gagcctatcc cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg      240

gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg      300

aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg      360

aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg      420

ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga      480

aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga      540

aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc      600

attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc      660

tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc cgctacgctg      720

atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg      780

ccacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac      840

aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag      900

gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaag         957


<210>  13
<211>  319
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TurboID

<400>  13

Lys Asp Asn Thr Val Pro Leu Lys Leu Ile Ala Leu Leu Ala Asn Gly 
1               5                   10                  15      


Glu Phe His Ser Gly Glu Gln Leu Gly Glu Thr Leu Gly Met Ser Arg 
            20                  25                  30          


Ala Ala Ile Asn Lys His Ile Gln Thr Leu Arg Asp Trp Gly Val Asp 
        35                  40                  45              


Val Phe Thr Val Pro Gly Lys Gly Tyr Ser Leu Pro Glu Pro Ile Pro 
    50                  55                  60                  


Leu Leu Asn Ala Lys Gln Ile Leu Gly Gln Leu Asp Gly Gly Ser Val 
65                  70                  75                  80  


Ala Val Leu Pro Val Val Asp Ser Thr Asn Gln Tyr Leu Leu Asp Arg 
                85                  90                  95      


Ile Gly Glu Leu Lys Ser Gly Asp Ala Cys Ile Ala Glu Tyr Gln Gln 
            100                 105                 110         


Ala Gly Arg Gly Ser Arg Gly Arg Lys Trp Phe Ser Pro Phe Gly Ala 
        115                 120                 125             


Asn Leu Tyr Leu Ser Met Phe Trp Arg Leu Lys Arg Gly Pro Ala Ala 
    130                 135                 140                 


Ile Gly Leu Gly Pro Val Ile Gly Ile Val Met Ala Glu Ala Leu Arg 
145                 150                 155                 160 


Lys Leu Gly Ala Asp Lys Val Arg Val Lys Trp Pro Asn Asp Leu Tyr 
                165                 170                 175     


Leu Gln Asp Arg Lys Leu Ala Gly Ile Leu Val Glu Leu Ala Gly Ile 
            180                 185                 190         


Thr Gly Asp Ala Ala Gln Ile Val Ile Gly Ala Gly Ile Asn Val Ala 
        195                 200                 205             


Met Arg Arg Val Glu Glu Ser Val Val Asn Gln Gly Trp Ile Thr Leu 
    210                 215                 220                 


Gln Glu Ala Gly Ile Asn Leu Asp Arg Asn Thr Leu Ala Ala Thr Leu 
225                 230                 235                 240 


Ile Arg Glu Leu Arg Ala Ala Leu Glu Leu Phe Glu Gln Glu Gly Leu 
                245                 250                 255     


Ala Pro Tyr Leu Pro Arg Trp Glu Lys Leu Asp Asn Phe Ile Asn Arg 
            260                 265                 270         


Pro Val Lys Leu Ile Ile Gly Asp Lys Glu Ile Phe Gly Ile Ser Arg 
        275                 280                 285             


Gly Ile Asp Lys Gln Gly Ala Leu Leu Leu Glu Gln Asp Gly Val Ile 
    290                 295                 300                 


Lys Pro Trp Met Gly Gly Glu Ile Ser Leu Arg Ser Ala Glu Lys 
305                 310                 315                 


<210>  14
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  myc localization sequence for transient mammalian cytosol 
       expression

<400>  14

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES

<400>  15

Leu Gln Leu Pro Pro Leu Glu Arg Leu Thr Leu Asp 
1               5                   10          


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage recognition site for TEV protease

<400>  16

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  17

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  myc localization sequence for inducible yeast cell surface 
       expression for yeast display

<400>  18

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  19
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5l localization sequence for transient mammalian cytosol 
       expression

<400>  19

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  20
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NES

<400>  20

Leu Gln Leu Pro Pro Leu Glu Arg Leu Thr Leu Asp 
1               5                   10          


<210>  21
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 localization sequence for transient mammalian nucleus 
       expression

<400>  21

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  22
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS

<400>  22

Asp Pro Lys Lys Lys Arg Lys Val Asp Pro Lys Lys Lys Arg Lys Val 
1               5                   10                  15      


Asp Pro Lys Lys Lys Arg Lys Val 
            20                  


<210>  23
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mitochondrial matrix targeting sequence from COX4

<400>  23

Met Leu Ala Thr Arg Val Phe Ser Leu Val Gly Lys Arg Ala Ile Ser 
1               5                   10                  15      


Thr Ser Val Cys Val Arg Ala His 
            20                  


<210>  24
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mitochondrial matrix targeting sequence from V5

<400>  24

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  25
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endoplasmic reticulum membrane anchor derived from cytochrome 
       P450

<400>  25

Met Asp Pro Val Val Val Leu Gly Leu Cys Leu Ser Cys Leu Leu Leu 
1               5                   10                  15      


Leu Ser Leu Trp Lys Gln Ser Tyr Gly Gly Gly 
            20                  25          


<210>  26
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  26

Gly Ser Gly Ser 
1               


<210>  27
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  27

Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10                  15      


Gly Ser Gly 
            


<210>  28
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 localization sequence for transient mammalian ER membrane 
       expression

<400>  28

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  29
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ig K-chain ss

<400>  29

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp 
            20      


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA localization sequence for transient mammalian ER lumen 
       expression

<400>  30

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  31
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 localization sequence for transient mammalian ER lumen 
       expression

<400>  31

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  32
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 sequence for inducible yeast cytosol expression

<400>  32

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  33
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His 6

<400>  33

His His His His His His 
1               5       


<210>  34
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  34

Gly Gly Gly Ser 
1               


<210>  35
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  35

Gly Gly Ser Ser Gly 
1               5   


<210>  36
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 sequence for Entry vector for Gateway LR recombination into 
       destination vector

<400>  36

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  37
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 sequence for generating trasngenic flies for Gal4-inducible 
       expression

<400>  37

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  38
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3xHA for intestinal expression

<400>  38

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Tyr Pro Tyr Asp Val Pro Asp 
1               5                   10                  15      


Tyr Ala Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
            20                  25          


