                         SEQUENCE LISTING

<110>  TSINGHUA UNIVERSITY
 
<120>  NR4A1 AS A KEY MEDIATOR OF T CELL TOLERANCE

<130>  PIDC3172897P

<160>  15    

<170>  PatentIn version 3.3

<210>  1
<211>  601
<212>  PRT
<213>  Artificial

<220>
<223>  amino acid sequence of the biomarker

<400>  1

Met Pro Cys Ile Gln Ala Gln Tyr Gly Thr Pro Ala Thr Ser Pro Gly 
1               5                   10                  15      


Pro Arg Asp His Leu Thr Gly Asp Pro Leu Ala Leu Glu Phe Gly Lys 
            20                  25                  30          


Pro Thr Met Asp Leu Ala Ser Pro Glu Thr Ala Pro Ala Ala Pro Ala 
        35                  40                  45              


Thr Leu Pro Ser Phe Ser Thr Phe Met Asp Gly Tyr Thr Gly Glu Phe 
    50                  55                  60                  


Asp Thr Phe Leu Tyr Gln Leu Pro Gly Thr Thr Gln Pro Cys Ser Ser 
65                  70                  75                  80  


Ala Cys Ser Ser Ala Ser Ser Thr Ser Ser Ser Ser Ser Ser Ala Thr 
                85                  90                  95      


Ser Pro Ala Ser Ala Ser Phe Lys Phe Glu Asp Phe Gln Val Tyr Gly 
            100                 105                 110         


Cys Tyr Pro Gly Thr Leu Ser Gly Pro Leu Asp Glu Thr Leu Ser Ser 
        115                 120                 125             


Ser Gly Ser Glu Tyr Tyr Gly Ser Pro Cys Ser Ala Pro Ser Pro Ser 
    130                 135                 140                 


Thr Pro Asn Phe Gln Pro Ser Gln Leu Ser Pro Trp Asp Gly Ser Phe 
145                 150                 155                 160 


Gly His Phe Ser Pro Ser Gln Thr Tyr Glu Gly Leu Trp Ala Trp Thr 
                165                 170                 175     


Glu Gln Leu Pro Lys Ala Ser Ser Gly Pro Pro Pro Pro Pro Thr Phe 
            180                 185                 190         


Phe Ser Phe Ser Pro Pro Thr Gly Pro Ser Pro Ser Leu Ala Gln Ser 
        195                 200                 205             


Ser Leu Lys Leu Phe Pro Pro Pro Ala Thr His Gln Leu Gly Glu Gly 
    210                 215                 220                 


Glu Ser Tyr Ser Met Pro Ala Ala Phe Pro Gly Leu Ala Pro Thr Ser 
225                 230                 235                 240 


Pro Asn Arg Asp Thr Ser Gly Ile Leu Asp Ala Pro Val Thr Ser Thr 
                245                 250                 255     


Lys Ser Arg Ser Gly Ala Ser Gly Gly Ser Glu Gly Arg Cys Ala Val 
            260                 265                 270         


Cys Gly Asp Asn Ala Ser Cys Gln His Tyr Gly Val Arg Thr Cys Glu 
        275                 280                 285             


Gly Cys Lys Gly Phe Phe Lys Arg Thr Val Gln Lys Ser Ala Lys Tyr 
    290                 295                 300                 


Ile Cys Leu Ala Asn Lys Asp Cys Pro Val Asp Lys Arg Arg Arg Asn 
305                 310                 315                 320 


Arg Cys Gln Phe Cys Arg Phe Gln Lys Cys Leu Ala Val Gly Met Val 
                325                 330                 335     


Lys Glu Val Val Arg Thr Asp Ser Leu Lys Gly Arg Arg Gly Arg Leu 
            340                 345                 350         


Pro Ser Lys Pro Lys Gln Pro Pro Asp Ala Ser Pro Thr Asn Leu Leu 
        355                 360                 365             


Thr Ser Leu Ile Arg Ala His Leu Asp Ser Gly Pro Ser Thr Ala Lys 
    370                 375                 380                 


Leu Asp Tyr Ser Lys Phe Gln Glu Leu Val Leu Pro Arg Phe Gly Lys 
385                 390                 395                 400 


Glu Asp Ala Gly Asp Val Gln Gln Phe Tyr Asp Leu Leu Ser Gly Ser 
                405                 410                 415     


Leu Asp Val Ile Arg Lys Trp Ala Glu Lys Ile Pro Gly Phe Ile Glu 
            420                 425                 430         


Leu Cys Pro Gly Asp Gln Asp Leu Leu Leu Glu Ser Ala Phe Leu Glu 
        435                 440                 445             


Leu Phe Ile Leu Arg Leu Ala Tyr Arg Ser Lys Pro Gly Glu Gly Lys 
    450                 455                 460                 


Leu Ile Phe Cys Ser Gly Leu Val Leu His Gln Leu Gln Cys Ala Arg 
465                 470                 475                 480 


Gly Phe Gly Asp Trp Ile Asp Asn Ile Leu Ala Phe Ser Arg Ser Leu 
                485                 490                 495     


His Ser Leu Gly Val Asp Val Pro Ala Phe Ala Cys Leu Ser Ala Leu 
            500                 505                 510         


Val Leu Ile Thr Asp Arg His Gly Leu Gln Asp Pro Arg Arg Val Glu 
        515                 520                 525             


Glu Leu Gln Asn Arg Ile Ala Ser Cys Leu Lys Glu His Met Ala Thr 
    530                 535                 540                 


Val Ala Gly Asp Pro Gln Pro Ala Ser Cys Leu Ser Arg Leu Leu Gly 
545                 550                 555                 560 


Lys Leu Pro Glu Leu Arg Thr Leu Cys Thr Gln Gly Leu Gln Arg Ile 
                565                 570                 575     


Phe Cys Leu Lys Leu Glu Asp Leu Val Pro Pro Pro Pro Ile Val Asp 
            580                 585                 590         


Lys Ile Phe Met Asp Thr Leu Ser Phe 
        595                 600     


<210>  2
<211>  8250
<212>  DNA
<213>  Artificial

<220>
<223>  a nucleotide sequence the construct having

<400>  2
cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc     60

cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc    120

tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc    180

ctccctttat ccagccctca ctccttctct aggcgccgga attagatctg atgccctgta    240

ttcaagctca atatggaaca ccagcaacga gcccaggacc gcgtgaccac ctgaccggtg    300

atcccctggc ccttgagttc ggcaagccta ccatggacct ggccagcccc gagacagcac    360

ctgccgcacc tgctacactg cccagcttca gcaccttcat ggacgggtac accggagagt    420

ttgacacctt cctctaccag ctgccgggga cgacccagcc gtgctcctca gcttgttcct    480

ctgcctcctc cacgtcttct tcctcatcct cggccacctc ccccgcttcg gcgtccttca    540

agtttgagga cttccaggtg tacggctgct acccgggcac cctgagcggc ccattagatg    600

agaccctatc ctccagcggc tctgagtact atggcagtcc ctgctcagcc ccctcgccat    660

ctacacccaa cttccagccg tcccagcttt ctccctggga cggctcattt ggccacttct    720

ccccgagcca gacttatgaa ggcctctggg catggacaga gcagttgcct aaggcttctt    780

cagggcctcc gccacctcca accttcttct ccttcagtcc tcccactggc cccagcccca    840

gcctggccca gagttctctg aaattgttcc caccaccagc cacccaccag cttggggagg    900

gggagagcta ttccatgcca gcagctttcc ccggcttggc acccacctct ccgaaccgtg    960

acacttccgg cattctggac gcacccgtga cctccaccaa gtcccggagc ggggcttcag   1020

gtggcagcga gggccgctgt gcagtctgtg gtgacaatgc ttcgtgtcag cactatgggg   1080

tccgcacctg tgagggctgc aagggcttct tcaagcgcac agtacagaaa agcgccaagt   1140

acatctgcct ggcaaacaag gattgccctg tggacaagag gcggcggaac cgctgccagt   1200

tctgccgctt ccagaagtgc ctggctgtgg gcatggtgaa ggaagttgta cggacagaca   1260

gcctaaaagg gcggcggggc cggctacctt caaaacccaa gcagcctcca gatgcctccc   1320

ctaccaatct tctcacttcc ctcatccggg cacacttgga ctccgggcct agcactgcca   1380

aattggacta ttccaagttc caggaactgg tgctgccccg cttcgggaag gaagatgccg   1440

gtgacgtgca acaattttat gacttgctct ctggttccct ggacgttatc cgaaagtggg   1500

cagaaaaaat ccctggcttc attgagcttt gcccaggaga ccaagacctg ttgctagagt   1560

ctgccttcct ggaactcttc atcctccgcc tggcataccg atctaaaccc ggtgagggga   1620

agctcatctt ctgctcaggc ctggtactac accagctgca gtgtgcccgt ggctttggtg   1680

attggattga caacatcctg gccttctcac ggtccctgca cagcttgggt gttgatgttc   1740

ccgcctttgc ctgcctgtcc gctctggtcc tcatcactga tcgacacggg ctccaggacc   1800

ctcgtcgggt ggaagagctg cagaatcgca ttgctagctg tctgaaggag cacatggcta   1860

ccgtggcagg agacccacag ccggccagct gcctgtcacg tctgctgggc aaactgcctg   1920

agcttcggac cctgtgcact caaggcctgc agcgcatctt ttgcctcaag ttggaggact   1980

tggtaccccc tccacctatt gtggacaaga tctttatgga cacattgtct ttctgacctc   2040

gaggcggccg cacgcgtgaa ttcggtatcg ataagcttga tatcgaattc cgcccccccc   2100

cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta   2160

ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc   2220

ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat   2280

gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc   2340

ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt   2400

gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt   2460

gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag   2520

aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt   2580

tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga   2640

aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg   2700

ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc   2760

tggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac   2820

cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg   2880

cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga   2940

aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc   3000

cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt   3060

caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt   3120

ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa   3180

catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga   3240

cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga   3300

ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac   3360

tctcggcatg gacgagctgt acaagtaatg agtcgacctg cagccaagct tatcgataaa   3420

ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt   3480

tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata   3540

gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat   3600

ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt   3660

cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga   3720

aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg   3780

cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc   3840

cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg   3900

acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg   3960

ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt   4020

cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact   4080

cctgaaatag ttcattatgg acagcgcaga aagagctggg gagaattgtg aaattgttat   4140

ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc   4200

taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga   4260

aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt   4320

attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg   4380

cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac   4440

gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg   4500

ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca   4560

agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc   4620

tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc   4680

ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag   4740

gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc   4800

ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca   4860

gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg   4920

aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg   4980

aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct   5040

ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa   5100

gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa   5160

gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa   5220

tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc   5280

ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga   5340

ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca   5400

atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc   5460

ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat   5520

tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc   5580

attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt   5640

tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc   5700

ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg   5760

gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt   5820

gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg   5880

gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga   5940

aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg   6000

taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg   6060

tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt   6120

tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc   6180

atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca   6240

tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat   6300

aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac   6360

ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc   6420

agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat   6480

gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga   6540

tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcagctgcgc aactgttggg   6600

aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg   6660

caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg   6720

cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc   6780

caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc   6840

atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc   6900

cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt   6960

ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata   7020

gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg   7080

taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg   7140

agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca   7200

ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga   7260

tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg   7320

acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt   7380

cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca   7440

gtcctccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct cttgctgttt   7500

gcatccgaat cgtggactcg ctgatccttg ggagggtctc ctcagattga ttgactgccc   7560

acctcggggg tctttcattt ggaggttcca ccgagatttg gagacccctg cccagggacc   7620

accgaccccc ccgccgggag gtaagctggc cagcggtcgt ttcgtgtctg tctctgtctt   7680

tgtgcgtgtt tgtgccggca tctaatgttt gcgcctgcgt ctgtactagt tagctaacta   7740

gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc   7800

tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc   7860

gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca   7920

gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct   7980

gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc   8040

tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga   8100

tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt   8160

ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg   8220

agacctcatc acccaggtta agatcaaggt                                    8250


<210>  3
<211>  63
<212>  DNA
<213>  Artificial

<220>
<223>  nucleotide sequence of the shRNA

<400>  3
ccggacatgt gcctttaagc ctatagttca agagactata ggcttaaagg cacatgtttt     60

ttg                                                                   63


<210>  4
<211>  63
<212>  DNA
<213>  Artificial

<220>
<223>  the nucleotide sequence of the shRNA

<400>  4
ccggatctgg ttccctggac gttatcttca agagagataa cgtccaggga accagatttt     60

ttg                                                                   63


<210>  5
<211>  63
<212>  DNA
<213>  Artificial

<220>
<223>  the nucleotide sequence of the  shRNA

<400>  5
ccggactatt gtggacaaga tctttattca agagataaag atcttgtcca caatagtttt     60

ttg                                                                   63


<210>  6
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Nr4a1, forward

<400>  6
agttggggga gtgtgctaga                                                 20


<210>  7
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Nr4a1, reverse

<400>  7
gcttgaatac agggcatctc cag                                             23


<210>  8
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Itch, forward

<400>  8
accctaaagt tgtggggatg g                                               21


<210>  9
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Itch, reverse

<400>  9
gtttggctga gatgacagtg a                                               21


<210>  10
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Klf4, forward

<400>  10
gactaaccgt tggcgtgagg                                                 20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Klf4, reverse

<400>  11
gtctaggtcc aggaggtcgt                                                 20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Hivep3, forward

<400>  12
ccccctagtt ctctgcctct                                                 20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Hivep3, reverse

<400>  13
gcttagatcg caggagggac                                                 20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Lpin2, forward

<400>  14
tgtttgtggt gccttcacct                                                 20


<210>  15
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Lpin2,reverse

<400>  15
tggtttgaga cagaatccca gg                                              22


