                         SEQUENCE LISTING

<110>  Telethon Kids Institute
       Phylogica Limited
 
<120>  Vaccine conjugates and uses thereof

<130>  M53015340

<150>  2018900032
<151>  2018-01-05

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site consensus


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  Xaa
<222>  (3)..(3)
<223>  Xaa can be Lys or Arg

<400>  1

Arg Xaa Xaa Arg 
1               


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Furin cleavage site

<400>  2

Arg Lys Lys Arg 
1               


<210>  3
<211>  2
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V-citrulline


<220>
<221>  Xaa
<222>  (2)..(2)
<223>  Xaa is citrulline

<400>  3

Val Xaa 
1       


<210>  4
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cathepsin B cleavage site

<400>  4

Phe Lys Phe Leu 
1               


<210>  5
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FCB


<220>
<221>  Xaa
<222>  (16)..(16)
<223>  Xaa is citrulline

<400>  5

Gly Ser Gly Thr Gly Gly Ser Thr Gly Arg Lys Lys Arg Ser Val Xaa 
1               5                   10                  15      


Gly Cys 
        


<210>  6
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1746

<400>  6

Pro Leu Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
1               5                   10                  15      


Pro Pro Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
            20                  25                  30          


Pro Pro Lys Pro Lys Arg 
        35              


<210>  7
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker peptide between moiety capable of endosomal escape and 
       antigen

<400>  7

Gly Ala Ser 
1           


<210>  8
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TAT

<400>  8

Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg Pro Gln 
1               5                   10          


<210>  9
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTag

<400>  9

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly 
1               5                   10                  


<210>  10
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyCatcher

<400>  10

Ser Tyr Tyr His His His His His His Asp Tyr Asp Ile Pro Thr Thr 
1               5                   10                  15      


Glu Asn Leu Tyr Phe Gln Gly Ala Met Val Asp Thr Leu Ser Gly Leu 
            20                  25                  30          


Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr Ile Glu Glu Asp Ser 
        35                  40                  45              


Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu 
    50                  55                  60                  


Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser 
65                  70                  75                  80  


Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly 
                85                  90                  95      


Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala 
            100                 105                 110         


Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn 
        115                 120                 125             


Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
    130                 135             


<210>  11
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XCL1

<400>  11

Val Gly Thr Glu Val Leu Glu Glu Ser Ser Cys Val Asn Leu Gln Thr 
1               5                   10                  15      


Gln Arg Leu Pro Val Gln Lys Ile Lys Thr Tyr Ile Ile Trp Glu Gly 
            20                  25                  30          


Ala Met Arg Ala Val Ile Phe Val Thr Lys Arg Gly Leu Lys Ile Cys 
        35                  40                  45              


Ala Asp Pro Glu Ala Lys Trp Val Lys Ala Ala Ile Lys Thr Val Asp 
    50                  55                  60                  


Gly Arg Ala Ser Thr Arg Lys Asn Met Ala Glu Thr Val Pro Thr Gly 
65                  70                  75                  80  


Ala Gln Arg Ser Thr Ser Thr Ala Ile Thr Leu Thr Gly 
                85                  90              


<210>  12
<211>  392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyCBLA

<400>  12

Met Gly His His His His His His Gly Ala Thr Leu Glu Val Leu Phe 
1               5                   10                  15      


Gln Gly Pro Gly Gly Ser Gly Ser Asp Ser Ala Thr His Ile Lys Phe 
            20                  25                  30          


Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu Ala Gly Ala Thr Met Glu 
        35                  40                  45              


Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp Ile Ser Asp Gly 
    50                  55                  60                  


Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr Thr Phe Val Glu 
65                  70                  75                  80  


Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Ala Ile Thr Phe Thr 
                85                  90                  95      


Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly Lys Ala Thr Lys Gly 
            100                 105                 110         


Ser His Pro Glu Thr Leu Val Lys Val Lys Asp Ala Glu Asp Gln Leu 
        115                 120                 125             


Gly Ala Arg Val Gly Tyr Ile Glu Leu Asp Leu Asn Ser Gly Lys Ile 
    130                 135                 140                 


Leu Glu Ser Phe Arg Pro Glu Glu Arg Phe Pro Met Met Ser Thr Phe 
145                 150                 155                 160 


Lys Val Leu Leu Cys Gly Ala Val Leu Ser Arg Ile Asp Ala Gly Gln 
                165                 170                 175     


Glu Gln Leu Gly Arg Arg Ile His Tyr Ser Gln Asn Asp Leu Val Glu 
            180                 185                 190         


Tyr Ser Pro Val Thr Glu Lys His Leu Thr Asp Gly Met Thr Val Arg 
        195                 200                 205             


Glu Leu Cys Ser Ala Ala Ile Thr Met Ser Asp Asn Thr Ala Ala Asn 
    210                 215                 220                 


Leu Leu Leu Thr Thr Ile Gly Gly Pro Lys Glu Leu Thr Ala Phe Leu 
225                 230                 235                 240 


His Asn Met Gly Asp His Val Thr Arg Leu Asp Arg Trp Glu Pro Glu 
                245                 250                 255     


Leu Asn Glu Ala Ile Pro Asn Asp Glu Arg Asp Thr Thr Met Pro Ala 
            260                 265                 270         


Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly Glu Leu Leu Thr 
        275                 280                 285             


Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp Met Glu Ala Asp Lys Val 
    290                 295                 300                 


Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro Ala Gly Trp Phe Ile Ala 
305                 310                 315                 320 


Asp Lys Ser Gly Ala Gly Glu Arg Gly Ser Arg Gly Ile Ile Ala Ala 
                325                 330                 335     


Leu Gly Pro Asp Gly Lys Pro Ser Arg Ile Val Val Ile Tyr Thr Thr 
            340                 345                 350         


Gly Ser Gln Ala Thr Met Asp Glu Arg Asn Arg Gln Ile Ala Glu Ile 
        355                 360                 365             


Gly Ala Ser Leu Ile Lys His Trp Gln Leu Gly Ser Ala Ser Gly Thr 
    370                 375                 380                 


Thr Gly Ala Thr Ser Gly Glu Phe 
385                 390         


<210>  13
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1746-SpyT

<400>  13

Pro Leu Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
1               5                   10                  15      


Pro Pro Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
            20                  25                  30          


Pro Pro Lys Pro Lys Arg Gly Ala Ser Ala His Ile Val Met Val Asp 
        35                  40                  45              


Ala Tyr Lys Pro Thr Lys Gly 
    50                  55  


<210>  14
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TATSpyT

<400>  14

Arg Gln Ile Lys Ile Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys 
1               5                   10                  15      


Gly Ala Ser Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
            20                  25                  30          


Gly 
    


<210>  15
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PYC35

<400>  15

Ala Tyr Gln Ser Ile Arg Ser Gly Gly Ile Glu Ser Ser Ser Lys Arg 
1               5                   10                  15      


Glu Arg 
        


<210>  16
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gD

<400>  16

Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala 
1               5                   10              


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gB

<400>  17

Ser Ser Ile Glu Phe Ala Arg Leu 
1               5               


<210>  18
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His6

<400>  18

His His His His His His 
1               5       


<210>  19
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GKPIPNPLLGLDST

<400>  19

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  20
<211>  225
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  muXCL1 _SpyC

<400>  20

Met Gly His His His His His His Gly Ala Thr Leu Glu Val Leu Phe 
1               5                   10                  15      


Gln Gly Pro Gly Gly Ser Val Gly Thr Glu Val Leu Glu Glu Ser Ser 
            20                  25                  30          


Cys Val Asn Leu Gln Thr Gln Arg Leu Pro Val Gln Lys Ile Lys Thr 
        35                  40                  45              


Tyr Ile Ile Trp Glu Gly Ala Met Arg Ala Val Ile Phe Val Thr Lys 
    50                  55                  60                  


Arg Gly Leu Lys Ile Cys Ala Asp Pro Glu Ala Lys Trp Val Lys Ala 
65                  70                  75                  80  


Ala Ile Lys Thr Val Asp Gly Arg Ala Ser Thr Arg Lys Asn Met Ala 
                85                  90                  95      


Glu Thr Val Pro Thr Gly Ala Gln Arg Ser Thr Ser Thr Ala Ile Thr 
            100                 105                 110         


Leu Thr Gly Gly Ser Gly Thr Gly Ala Thr Ser Gly Asp Ser Ala Thr 
        115                 120                 125             


His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu Ala Gly 
    130                 135                 140                 


Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp 
145                 150                 155                 160 


Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr 
                165                 170                 175     


Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Ala 
            180                 185                 190         


Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly Lys 
        195                 200                 205             


Ala Thr Lys Gly Gly Ala Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
    210                 215                 220                 


Gly 
225 


<210>  21
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5-SpyTag-1746-gDgB

<400>  21

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly Ala 
1               5                   10                  15      


Ser Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Cys 
            20                  25                  30          


Pro Leu Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
        35                  40                  45              


Pro Pro Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
    50                  55                  60                  


Pro Pro Lys Pro Lys Arg Gly Ala Ser Ile Pro Pro Asn Trp His Ile 
65                  70                  75                  80  


Pro Ser Ile Gln Asp Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala Arg 
                85                  90                  95      


Leu 
    


<210>  22
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5-SpyTag-PYC35-gDgB

<400>  22

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly Ala 
1               5                   10                  15      


Ser Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Cys 
            20                  25                  30          


Ala Tyr Gln Ser Ile Arg Ser Gly Gly Ile Glu Ser Ser Ser Lys Arg 
        35                  40                  45              


Glu Arg Gly Ala Ser Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln 
    50                  55                  60                  


Asp Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala Arg Leu 
65                  70                  75          


<210>  23
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5-SpyTag-TATgDgB

<400>  23

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly Ala 
1               5                   10                  15      


Ser Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Cys 
            20                  25                  30          


Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg Pro Gln Gly Ala Ser Ile 
        35                  40                  45              


Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly Thr Gly Ser 
    50                  55                  60                  


Ser Ile Glu Phe Ala Arg Leu 
65                  70      


<210>  24
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcpa- ATgDgB

<400>  24

Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg Pro Gln Gly Ala Ser Ile 
1               5                   10                  15      


Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly Thr Gly Ser 
            20                  25                  30          


Ser Ile Glu Phe Ala Arg Leu 
        35                  


<210>  25
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcpa-AVIgDgB

<400>  25

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Phe His Glu Gly 
1               5                   10                  15      


Ala Ser Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly 
            20                  25                  30          


Thr Gly Ser Ser Ile Glu Phe Ala Arg Leu 
        35                  40          


<210>  26
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTFCB-Cys

<400>  26

Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser 
1               5                   10                  15      


Gly Thr Gly Gly Ser Thr Gly Arg Lys Lys Arg Ser Val Gly Cys 
            20                  25                  30      


<210>  27
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1746-gDgB

<400>  27

Pro Leu Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
1               5                   10                  15      


Pro Pro Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
            20                  25                  30          


Pro Pro Lys Pro Lys Arg Gly Ala Ser Ile Pro Pro Asn Trp His Ile 
        35                  40                  45              


Pro Ser Ile Gln Asp Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala Arg 
    50                  55                  60                  


Leu 
65  


<210>  28
<211>  65
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcpa-1746-gDgB

<400>  28

Pro Leu Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
1               5                   10                  15      


Pro Pro Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln 
            20                  25                  30          


Pro Pro Lys Pro Lys Arg Gly Ala Ser Ile Pro Pro Asn Trp His Ile 
        35                  40                  45              


Pro Ser Ile Gln Asp Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala Arg 
    50                  55                  60                  


Leu 
65  


<210>  29
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcpa-gDgB

<400>  29

Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly Thr Gly 
1               5                   10                  15      


Ser Ser Ile Glu Phe Ala Arg Leu 
            20                  


<210>  30
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mcpa-MEMgDgB

<400>  30

Tyr Gly Thr Ile Ala Val Ile Val Phe Ala Leu Ile Gly Phe Thr Ile 
1               5                   10                  15      


Gly Tyr Leu Gly Tyr Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln 
            20                  25                  30          


Asp Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala Arg Leu 
        35                  40                  45  


<210>  31
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gDgB

<400>  31

Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly Thr Gly 
1               5                   10                  15      


Ser Ser Ile Glu Phe Ala Arg Leu 
            20                  


<210>  32
<211>  70
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTFCBTATgDgB

<400>  32

Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser 
1               5                   10                  15      


Gly Thr Gly Gly Ser Thr Gly Arg Lys Lys Arg Ser Val Gly Cys Gly 
            20                  25                  30          


Arg Lys Lys Arg Arg Gln Arg Arg Arg Pro Gln Gly Ala Ser Ile Pro 
        35                  40                  45              


Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly Thr Gly Ser Ser 
    50                  55                  60                  


Ile Glu Phe Ala Arg Leu 
65                  70  


<210>  33
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTFCB-AvigDgB

<400>  33

Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser 
1               5                   10                  15      


Gly Thr Gly Gly Ser Thr Gly Arg Lys Lys Arg Ser Val Gly Cys Gly 
            20                  25                  30          


Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Phe His Glu Gly Ala 
        35                  40                  45              


Ser Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly Thr 
    50                  55                  60                  


Gly Ser Ser Ile Glu Phe Ala Arg Leu 
65                  70              


<210>  34
<211>  96
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTFCB-1746-gDgB

<400>  34

Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser 
1               5                   10                  15      


Gly Thr Gly Gly Ser Thr Gly Arg Lys Lys Arg Ser Val Gly Cys Pro 
            20                  25                  30          


Leu Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln Pro 
        35                  40                  45              


Pro Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys Gln Pro 
    50                  55                  60                  


Pro Lys Pro Lys Arg Gly Ala Ser Ile Pro Pro Asn Trp His Ile Pro 
65                  70                  75                  80  


Ser Ile Gln Asp Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala Arg Leu 
                85                  90                  95      


<210>  35
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTFCBgDgB

<400>  35

Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser 
1               5                   10                  15      


Gly Thr Gly Gly Ser Thr Gly Arg Lys Lys Arg Ser Val Gly Cys Ile 
            20                  25                  30          


Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly Thr Gly Ser 
        35                  40                  45              


Ser Ile Glu Phe Ala Arg Leu 
    50                  55  


<210>  36
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyTFCBMEMgDgB

<400>  36

Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser 
1               5                   10                  15      


Gly Thr Gly Gly Ser Thr Gly Arg Lys Lys Arg Ser Val Gly Cys Tyr 
            20                  25                  30          


Gly Thr Ile Ala Val Ile Val Phe Ala Leu Ile Gly Phe Thr Ile Gly 
        35                  40                  45              


Tyr Leu Gly Tyr Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp 
    50                  55                  60                  


Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala Arg Leu 
65                  70                  75      


<210>  37
<211>  322
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  muXCL1_SpyC+V5-SpyTag-1746-gDgB

<400>  37

Met Gly His His His His His His Gly Ala Thr Leu Glu Val Leu Phe 
1               5                   10                  15      


Gln Gly Pro Gly Gly Ser Val Gly Thr Glu Val Leu Glu Glu Ser Ser 
            20                  25                  30          


Cys Val Asn Leu Gln Thr Gln Arg Leu Pro Val Gln Lys Ile Lys Thr 
        35                  40                  45              


Tyr Ile Ile Trp Glu Gly Ala Met Arg Ala Val Ile Phe Val Thr Lys 
    50                  55                  60                  


Arg Gly Leu Lys Ile Cys Ala Asp Pro Glu Ala Lys Trp Val Lys Ala 
65                  70                  75                  80  


Ala Ile Lys Thr Val Asp Gly Arg Ala Ser Thr Arg Lys Asn Met Ala 
                85                  90                  95      


Glu Thr Val Pro Thr Gly Ala Gln Arg Ser Thr Ser Thr Ala Ile Thr 
            100                 105                 110         


Leu Thr Gly Gly Ser Gly Thr Gly Ala Thr Ser Gly Asp Ser Ala Thr 
        115                 120                 125             


His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu Ala Gly 
    130                 135                 140                 


Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp 
145                 150                 155                 160 


Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr 
                165                 170                 175     


Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Ala 
            180                 185                 190         


Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly Lys 
        195                 200                 205             


Ala Thr Lys Gly Gly Ala Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
    210                 215                 220                 


Gly Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly 
225                 230                 235                 240 


Ala Ser Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly 
                245                 250                 255     


Cys Pro Leu Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys 
            260                 265                 270         


Gln Pro Pro Lys Pro Lys Lys Pro Lys Thr Gln Glu Lys Lys Lys Lys 
        275                 280                 285             


Gln Pro Pro Lys Pro Lys Arg Gly Ala Ser Ile Pro Pro Asn Trp His 
    290                 295                 300                 


Ile Pro Ser Ile Gln Asp Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala 
305                 310                 315                 320 


Arg Leu 
        


<210>  38
<211>  302
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  muXCL1_SpyC+V5-SpyTag-PYC35-gDgB

<400>  38

Met Gly His His His His His His Gly Ala Thr Leu Glu Val Leu Phe 
1               5                   10                  15      


Gln Gly Pro Gly Gly Ser Val Gly Thr Glu Val Leu Glu Glu Ser Ser 
            20                  25                  30          


Cys Val Asn Leu Gln Thr Gln Arg Leu Pro Val Gln Lys Ile Lys Thr 
        35                  40                  45              


Tyr Ile Ile Trp Glu Gly Ala Met Arg Ala Val Ile Phe Val Thr Lys 
    50                  55                  60                  


Arg Gly Leu Lys Ile Cys Ala Asp Pro Glu Ala Lys Trp Val Lys Ala 
65                  70                  75                  80  


Ala Ile Lys Thr Val Asp Gly Arg Ala Ser Thr Arg Lys Asn Met Ala 
                85                  90                  95      


Glu Thr Val Pro Thr Gly Ala Gln Arg Ser Thr Ser Thr Ala Ile Thr 
            100                 105                 110         


Leu Thr Gly Gly Ser Gly Thr Gly Ala Thr Ser Gly Asp Ser Ala Thr 
        115                 120                 125             


His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu Ala Gly 
    130                 135                 140                 


Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp 
145                 150                 155                 160 


Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr 
                165                 170                 175     


Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Ala 
            180                 185                 190         


Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly Lys 
        195                 200                 205             


Ala Thr Lys Gly Gly Ala Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
    210                 215                 220                 


Gly Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly 
225                 230                 235                 240 


Ala Ser Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly 
                245                 250                 255     


Cys Ala Tyr Gln Ser Ile Arg Ser Gly Gly Ile Glu Ser Ser Ser Lys 
            260                 265                 270         


Arg Glu Arg Gly Ala Ser Ile Pro Pro Asn Trp His Ile Pro Ser Ile 
        275                 280                 285             


Gln Asp Ala Gly Thr Gly Ser Ser Ile Glu Phe Ala Arg Leu 
    290                 295                 300         


<210>  39
<211>  296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  muXCL1_SpyC+V5-SpyTag-TATgDgB

<400>  39

Met Gly His His His His His His Gly Ala Thr Leu Glu Val Leu Phe 
1               5                   10                  15      


Gln Gly Pro Gly Gly Ser Val Gly Thr Glu Val Leu Glu Glu Ser Ser 
            20                  25                  30          


Cys Val Asn Leu Gln Thr Gln Arg Leu Pro Val Gln Lys Ile Lys Thr 
        35                  40                  45              


Tyr Ile Ile Trp Glu Gly Ala Met Arg Ala Val Ile Phe Val Thr Lys 
    50                  55                  60                  


Arg Gly Leu Lys Ile Cys Ala Asp Pro Glu Ala Lys Trp Val Lys Ala 
65                  70                  75                  80  


Ala Ile Lys Thr Val Asp Gly Arg Ala Ser Thr Arg Lys Asn Met Ala 
                85                  90                  95      


Glu Thr Val Pro Thr Gly Ala Gln Arg Ser Thr Ser Thr Ala Ile Thr 
            100                 105                 110         


Leu Thr Gly Gly Ser Gly Thr Gly Ala Thr Ser Gly Asp Ser Ala Thr 
        115                 120                 125             


His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu Ala Gly 
    130                 135                 140                 


Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp 
145                 150                 155                 160 


Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr 
                165                 170                 175     


Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Ala 
            180                 185                 190         


Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly Lys 
        195                 200                 205             


Ala Thr Lys Gly Gly Ala Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
    210                 215                 220                 


Gly Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr Gly 
225                 230                 235                 240 


Ala Ser Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly 
                245                 250                 255     


Cys Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg Pro Gln Gly Ala Ser 
            260                 265                 270         


Ile Pro Pro Asn Trp His Ile Pro Ser Ile Gln Asp Ala Gly Thr Gly 
        275                 280                 285             


Ser Ser Ile Glu Phe Ala Arg Leu 
    290                 295     


<210>  40
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DNA insert for pET28a+muXCL1-SpyC

<400>  40

Cys Cys Ala Thr Gly Gly Gly Thr Cys Ala Thr Cys Ala Thr Cys Ala 
1               5                   10                  15      


Thr Cys Ala Thr Cys Ala Thr Cys Ala Cys Gly Gly Cys Gly Cys Ala 
            20                  25                  30          


Ala Cys Thr Cys Thr Gly Gly Ala Ala Gly Thr Thr Thr Thr Gly Thr 
        35                  40                  45              


Thr Thr Cys Ala Ala Gly Gly Thr Cys Cys Thr Gly Gly Cys Gly Gly 
    50                  55                  60                  


Cys Ala Gly Cys Gly Thr Thr Gly Gly Thr Ala Cys Cys Gly Ala Ala 
65                  70                  75                  80  


Gly Thr Gly Cys Thr Gly Gly Ala Ala Gly Ala Gly Ala Gly Cys Thr 
                85                  90                  95      


Cys Cys Thr Gly Cys Gly Thr Cys Ala Ala Thr Cys Thr Gly Cys Ala 
            100                 105                 110         


Ala Ala Cys Cys Cys Ala Ala Cys Gly Cys Cys Thr Gly Cys Cys Gly 
        115                 120                 125             


Gly Thr Gly Cys Ala Gly Ala Ala Ala Ala Thr Cys Ala Ala Ala Ala 
    130                 135                 140                 


Cys Cys Thr Ala Thr Ala Thr Cys Ala Thr Cys Thr Gly Gly Gly Ala 
145                 150                 155                 160 


Gly Gly Gly Thr Gly Cys Gly Ala Thr Gly Cys Gly Thr Gly Cys Cys 
                165                 170                 175     


Gly Thr Cys Ala Thr Thr Thr Thr Cys Gly Thr Ala Ala Cys Cys Ala 
            180                 185                 190         


Ala Gly Cys Gly Thr Gly Gly Thr Cys Thr Gly Ala Ala Gly Ala Thr 
        195                 200                 205             


Thr Thr Gly Thr Gly Cys Gly Gly Ala Cys Cys Cys Ala Gly Ala Gly 
    210                 215                 220                 


Gly Cys Gly Ala Ala Ala Thr Gly Gly Gly Thr Thr Ala Ala Ala Gly 
225                 230                 235                 240 


Cys Thr Gly Cys Cys Ala Thr Cys Ala Ala Ala Ala Cys Cys Gly Thr 
                245                 250                 255     


Thr Gly Ala Cys Gly Gly Cys Cys Gly Thr Gly Cys Gly Ala Gly Cys 
            260                 265                 270         


Ala Cys Gly Cys Gly Thr Ala Ala Gly Ala Ala Cys Ala Thr Gly Gly 
        275                 280                 285             


Cys Ala Gly Ala Gly Ala Cys Thr Gly Thr Gly Cys Cys Gly Ala Cys 
    290                 295                 300                 


Cys Gly Gly Thr Gly Cys Cys Cys Ala Gly Cys Gly Cys Thr Cys Thr 
305                 310                 315                 320 


Ala Cys Gly Ala Gly Cys Ala Cys Cys Gly Cys Gly Ala Thr Cys Ala 
                325                 330                 335     


Cys Cys Cys Thr Gly Ala Cys Cys Gly Gly Thr Gly Gly Cys Ala Gly 
            340                 345                 350         


Cys Gly Gly Cys Ala Cys Gly Gly Gly Thr Gly Cys Gly Ala Cys Cys 
        355                 360                 365             


Thr Cys Gly Gly Gly Cys Gly Ala Thr Ala Gly Cys Gly Cys Ala Ala 
    370                 375                 380                 


Cys Gly Cys Ala Cys Ala Thr Cys Ala Ala Ala Thr Thr Cys Thr Cys 
385                 390                 395                 400 


Thr Ala Ala Gly Cys Gly Cys Gly Ala Cys Gly Ala Ala Gly Ala Thr 
                405                 410                 415     


Gly Gly Cys Ala Ala Ala Gly Ala Ala Cys Thr Gly Gly Cys Gly Gly 
            420                 425                 430         


Gly Thr Gly Cys Gly Ala Cys Gly Ala Thr Gly Gly Ala Ala Cys Thr 
        435                 440                 445             


Gly Cys Gly Thr Gly Ala Thr Ala Gly Cys Ala Gly Cys Gly Gly Cys 
    450                 455                 460                 


Ala Ala Gly Ala Cys Gly Ala Thr Thr Thr Cys Cys Ala Cys Gly Thr 
465                 470                 475                 480 


Gly Gly Ala Thr Thr Ala Gly Cys Gly Ala Thr Gly Gly Cys Cys Ala 
                485                 490                 495     


Gly Gly Thr Cys Ala Ala Ala Gly Ala Cys Thr Thr Cys Thr Ala Thr 
            500                 505                 510         


Thr Thr Gly Thr Ala Cys Cys Cys Gly Gly Gly Thr Ala Ala Gly Thr 
        515                 520                 525             


Ala Cys Ala Cys Gly Thr Thr Thr Gly Thr Gly Gly Ala Gly Ala Cys 
    530                 535                 540                 


Thr Gly Cys Gly Gly Cys Thr Cys Cys Gly Gly Ala Cys Gly Gly Thr 
545                 550                 555                 560 


Thr Ala Cys Gly Ala Ala Gly Thr Thr Gly Cys Ala Ala Cys Cys Gly 
                565                 570                 575     


Cys Cys Ala Thr Thr Ala Cys Cys Thr Thr Cys Ala Cys Cys Gly Thr 
            580                 585                 590         


Gly Ala Ala Cys Gly Ala Gly Cys Ala Ala Gly Gly Thr Cys Ala Gly 
        595                 600                 605             


Gly Thr Cys Ala Cys Cys Gly Thr Thr Ala Ala Thr Gly Gly Thr Ala 
    610                 615                 620                 


Ala Gly Gly Cys Ala Ala Cys Cys Ala Ala Gly Gly Gly Thr Gly Gly 
625                 630                 635                 640 


Cys Gly Cys Thr Gly Gly Thr Ala Gly Cys Thr Gly Gly Ala Gly Cys 
                645                 650                 655     


Cys Ala Cys Cys Cys Gly Cys Ala Gly Thr Thr Thr Gly Ala Gly Ala 
            660                 665                 670         


Ala Ala Gly Gly Thr Thr Ala Ala Ala Thr Gly Ala Thr Thr Ala Ala 
        675                 680                 685             


Cys Thr Cys Gly Ala Gly 
    690                 


