                         SEQUENCE LISTING

<110>  Oncorus, Inc.
       Finer, Mitchell H.
       Evnin, Luke
 
<120>  ONCOLYTIC VIRAL DELIVERY OF THERAPEUTIC POLYPEPTIDES

<130>  ONCR-011/00US 324865-2068

<160>  80    

<170>  PatentIn version 3.5

<210>  1
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding signal peptide

<400>  1
atggagttcg gcctgagctg ggtgttcctg gtggccctgt tcaggggcgt gcagtgc          57


<210>  2
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized signal peptide sequence

<400>  2

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp 
            20  


<210>  3
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human GM-CSF leader sequence

<400>  3

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser 
            20  


<210>  4
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of human GM-CSFR leader sequence

<400>  4

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser 
            20  


<210>  5
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding 4GlySer linker peptide

<400>  5
ggcggcggcg gcagc                                                        15


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized 4GlySer linker peptide sequence

<400>  6

Gly Gly Gly Gly Ser 
1               5   


<210>  7
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding 3x 4GlySer linker peptide

<400>  7
ggcggcggcg gcagcggcgg cggcggcagc ggcggcggcg gcagc                       45


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized 3x 4GlySer linker peptide sequence

<400>  8

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  9
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding 4x 2GlySer linker peptide

<400>  9
gggcggcagc ggcggcagcg gcggcagcgg cggcagc                                37


<210>  10
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized 4x 2GlySer linker peptide sequence

<400>  10

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
1               5                   10          


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding 6-His tag

<400>  11
caccaccacc accaccac                                                     18


<210>  12
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized 6-His tag sequence

<400>  12

His His His His His His 
1               5       


<210>  13
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding T2A linker

<400>  13
cgaagaaaac gcgaggggag gggctccctg ctcacctgtg gtgatgtcga agagaaccct       60

gggccg                                                                  66


<210>  14
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized T2A linker linker sequence

<400>  14

Arg Arg Lys Arg Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  15
<211>  333
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding anti-CD19 light chain 
       sequence

<400>  15
gacatccagc tgacccagag ccccgccagc ctggccgtga gcctgggcca gagggccacc       60

atcagctgca aggccagcca gagcgtggac tacgacggcg acagctacct gaactggtac      120

cagcagatcc ccggccagcc ccccaagctg ctgatctacg acgccagcaa cctggtgagc      180

ggcatccccc ccaggttcag cggcagcggc agcggcaccg acttcaccct gaacatccac      240

cccgtggaga aggtggacgc cgccacctac cactgccagc agagcaccga ggacccctgg      300

accttcggcg gcggcaccaa gctggagatc aag                                   333


<210>  16
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized anti-CD19 light chain sequence

<400>  16

Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile Pro Pro 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln Gln Ser Thr 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  17
<211>  372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding anti-CD19 heavy chain 
       sequence

<400>  17
caggtgcagc tgcagcagag cggcgccgag ctggtgaggc ccggcagcag cgtgaagatc       60

agctgcaagg ccagcggcta cgccttcagc agctactgga tgaactgggt gaagcagagg      120

cccggccagg gcctggagtg gatcggccag atctggcccg gcgacggcga caccaactac      180

aacggcaagt tcaagggcaa ggccaccctg accgccgacg agagcagcag caccgcctac      240

atgcagctga gcagcctggc cagcgaggac agcgccgtgt acttctgcgc caggagggag      300

accaccaccg tgggcaggta ctactacgcc atggactact ggggccaggg caccaccgtg      360

accgtgagca gc                                                          372


<210>  18
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized anti-CD19 heavy chain sequence

<400>  18

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 


<210>  19
<211>  318
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding anti-CD3 light chain sequence

<400>  19
gacatccagc tgacccagag ccccgccatc atgagcgcca gccccggcga gaaggtgacc       60

atgacctgca gggccagcag cagcgtgagc tacatgaact ggtaccagca gaagagcggc      120

accagcccca agaggtggat ctacgacacc agcaaggtgg ccagcggcgt gccctacagg      180

ttcagcggca gcggcagcgg caccagctac agcctgacca tcagcagcat ggaggccgag      240

gacgccgcca cctactactg ccagcagtgg agcagcaacc ccctgacctt cggcgccggc      300

accaagctgg agctgaag                                                    318


<210>  20
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized anti-CD3 light chain sequence

<400>  20

Asp Ile Gln Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Asn Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105     


<210>  21
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding anti-CD3 heavy chain sequence

<400>  21
gacatcaagc tgcagcagag cggcgccgag ctggccaggc ccggcgccag cgtgaagatg       60

agctgcaaga ccagcggcta caccttcacc aggtacacca tgcactgggt gaagcagagg      120

cccggccagg gcctggagtg gatcggctac atcaacccca gcaggggcta caccaactac      180

aaccagaagt tcaaggacaa ggccaccctg accaccgaca agagcagcag caccgcctac      240

atgcagctga gcagcctgac cagcgaggac agcgccgtgt actactgcgc caggtactac      300

gacgaccact actgcctgga ctactggggc cagggcacca ccctgaccgt gagcagc         357


<210>  22
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized anti-CD3 heavy chain sequence

<400>  22

Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  23
<211>  489
<212>  DNA
<213>  Homo sapiens

<400>  23
atgagaattt cgaaaccaca tttgagaagt atttccatcc agtgctactt gtgtttactt       60

ctaaacagtc attttctaac tgaagctggc attcatgtct tcattttggg ctgtttcagt      120

gcagggcttc ctaaaacaga agccaactgg gtgaatgtaa taagtgattt gaaaaaaatt      180

gaagatctta ttcaatctat gcatattgat gctactttat atacggaaag tgatgttcac      240

cccagttgca aagtaacagc aatgaagtgc tttctcttgg agttacaagt tatttcactt      300

gagtccggag atgcaagtat tcatgataca gtagaaaatc tgatcatcct agcaaacaac      360

agtttgtctt ctaatgggaa tgtaacagaa tctggatgca aagaatgtga ggaactggag      420

gaaaaaaata ttaaagaatt tttgcagagt tttgtacata ttgtccaaat gttcatcaac      480

acttcttag                                                              489


<210>  24
<211>  162
<212>  PRT
<213>  Homo sapiens

<400>  24

Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr 
1               5                   10                  15      


Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His 
            20                  25                  30          


Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala 
        35                  40                  45              


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
    50                  55                  60                  


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
65                  70                  75                  80  


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
                85                  90                  95      


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
            100                 105                 110         


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
        115                 120                 125             


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
    130                 135                 140                 


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
145                 150                 155                 160 


Thr Ser 
        


<210>  25
<211>  987
<212>  DNA
<213>  Homo sapiens

<400>  25
atgtgtcacc agcagttggt catctcttgg ttttccctgg tttttctggc atctcccctc       60

gtggccatat gggaactgaa gaaagatgtt tatgtcgtag aattggattg gtatccggat      120

gcccctggag aaatggtggt cctcacctgt gacacccctg aagaagatgg tatcacctgg      180

accttggacc agagcagtga ggtcttaggc tctggcaaaa ccctgaccat ccaagtcaaa      240

gagtttggag atgctggcca gtacacctgt cacaaaggag gcgaggttct aagccattcg      300

ctcctgctgc ttcacaaaaa ggaagatgga atttggtcca ctgatatttt aaaggaccag      360

aaagaaccca aaaataagac ctttctaaga tgcgaggcca agaattattc tggacgtttc      420

acctgctggt ggctgacgac aatcagtact gatttgacat tcagtgtcaa aagcagcaga      480

ggctcttctg acccccaagg ggtgacgtgc ggagctgcta cactctctgc agagagagtc      540

agaggggaca acaaggagta tgagtactca gtggagtgcc aggaggacag tgcctgccca      600

gctgctgagg agagtctgcc cattgaggtc atggtggatg ccgttcacaa gctcaagtat      660

gaaaactaca ccagcagctt cttcatcagg gacatcatca aacctgaccc acccaagaac      720

ttgcagctga agccattaaa gaattctcgg caggtggagg tcagctggga gtaccctgac      780

acctggagta ctccacattc ctacttctcc ctgacattct gcgttcaggt ccagggcaag      840

agcaagagag aaaagaaaga tagagtcttc acggacaaga cctcagccac ggtcatctgc      900

cgcaaaaatg ccagcattag cgtgcgggcc caggaccgct actatagctc atcttggagc      960

gaatgggcat ctgtgccctg cagttag                                          987


<210>  26
<211>  329
<212>  PRT
<213>  Homo sapiens

<400>  26

Pro Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe 
1               5                   10                  15      


Leu Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr 
            20                  25                  30          


Val Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val 
        35                  40                  45              


Leu Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp 
    50                  55                  60                  


Gln Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val 
65                  70                  75                  80  


Lys Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu 
                85                  90                  95      


Val Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile 
            100                 105                 110         


Trp Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr 
        115                 120                 125             


Phe Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp 
    130                 135                 140                 


Trp Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser 
145                 150                 155                 160 


Arg Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu 
                165                 170                 175     


Ser Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val 
            180                 185                 190         


Glu Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro 
        195                 200                 205             


Ile Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr 
    210                 215                 220                 


Thr Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys 
225                 230                 235                 240 


Asn Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser 
                245                 250                 255     


Trp Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu 
            260                 265                 270         


Thr Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp 
        275                 280                 285             


Arg Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn 
    290                 295                 300                 


Ala Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp 
305                 310                 315                 320 


Ser Glu Trp Ala Ser Val Pro Cys Ser 
                325                 


<210>  27
<211>  762
<212>  DNA
<213>  Homo sapiens

<400>  27
atgtggcccc ctgggtcagc ctcccagcca ccgccctcac ctgccgcggc cacaggtctg       60

catccagcgg ctcgccctgt gtccctgcag tgccggctca gcatgtgtcc agcgcgcagc      120

ctcctccttg tggctaccct ggtcctcctg gaccacctca gtttggccag aaacctcccc      180

gtggccactc cagacccagg aatgttccca tgccttcacc actcccaaaa cctgctgagg      240

gccgtcagca acatgctcca gaaggccaga caaactctag aattttaccc ttgcacttct      300

gaagagattg atcatgaaga tatcacaaaa gataaaacca gcacagtgga ggcctgttta      360

ccattggaat taaccaagaa tgagagttgc ctaaattcca gagagacctc tttcataact      420

aatgggagtt gcctggcctc cagaaagacc tcttttatga tggccctgtg ccttagtagt      480

atttatgaag acttgaagat gtaccaggtg gagttcaaga ccatgaatgc aaagcttctg      540

atggatccta agaggcagat ctttctagat caaaacatgc tggcagttat tgatgagctg      600

atgcaggccc tgaatttcaa cagtgagact gtgccacaaa aatcctccct tgaagaaccg      660

gatttttata aaactaaaat caagctctgc atacttcttc atgctttcag aattcgggca      720

gtgactattg atagagtgat gagctatctg aatgcttcct ag                         762


<210>  28
<211>  253
<212>  PRT
<213>  Homo sapiens

<400>  28

Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala 
1               5                   10                  15      


Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg 
            20                  25                  30          


Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val 
        35                  40                  45              


Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro 
    50                  55                  60                  


Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg 
65                  70                  75                  80  


Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr 
                85                  90                  95      


Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys 
            100                 105                 110         


Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu 
        115                 120                 125             


Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys 
    130                 135                 140                 


Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser 
145                 150                 155                 160 


Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn 
                165                 170                 175     


Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn 
            180                 185                 190         


Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser 
        195                 200                 205             


Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys 
    210                 215                 220                 


Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala 
225                 230                 235                 240 


Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser 
                245                 250             


<210>  29
<211>  297
<212>  DNA
<213>  Homo sapiens

<400>  29
atgaatcaaa ctgccattct gatttgctgc cttatctttc tgactctaag tggcattcaa       60

ggagtacctc tctctagaac tgtacgctgt acctgcatca gcattagtaa tcaacctgtt      120

aatccaaggt ctttagaaaa acttgaaatt attcctgcaa gccaattttg tccacgtgtt      180

gagatcattg ctacaatgaa aaagaagggt gagaagagat gtctgaatcc agaatcgaag      240

gccatcaaga atttactgaa agcagttagc aaggaaaggt ctaaaagatc tccttag         297


<210>  30
<211>  98
<212>  PRT
<213>  Homo sapiens

<400>  30

Met Asn Gln Thr Ala Ile Leu Ile Cys Cys Leu Ile Phe Leu Thr Leu 
1               5                   10                  15      


Ser Gly Ile Gln Gly Val Pro Leu Ser Arg Thr Val Arg Cys Thr Cys 
            20                  25                  30          


Ile Ser Ile Ser Asn Gln Pro Val Asn Pro Arg Ser Leu Glu Lys Leu 
        35                  40                  45              


Glu Ile Ile Pro Ala Ser Gln Phe Cys Pro Arg Val Glu Ile Ile Ala 
    50                  55                  60                  


Thr Met Lys Lys Lys Gly Glu Lys Arg Cys Leu Asn Pro Glu Ser Lys 
65                  70                  75                  80  


Ala Ile Lys Asn Leu Leu Lys Ala Val Ser Lys Glu Arg Ser Lys Arg 
                85                  90                  95      


Ser Pro 
        


<210>  31
<211>  426
<212>  DNA
<213>  Homo sapiens

<400>  31
atggagaccg ataccctgct cttgtgggtt ttgcttcttt gggtgccagg atctacaggt       60

gatgaagaag aattgcagat catccaacca gacaaatccg tactcgtggc cgcaggagag      120

accgctaccc tcagatgtac catcacttct ctcttccccg ttggccccat ccagtggttt      180

cgaggcgcag gaccaggacg agtgcttatt tacaatcaac gacagggccc attcccaaga      240

gtgacaacag tatccgatac caccaagcgc aataatatgg actttagcat tagaatcggc      300

aacataacac ccgctgacgc cggtacatac tattgtatta aatttcgaaa gggctcacca      360

gacgacgtgg aatttaagtc aggggccgga accgaactct cagttagagc aaaaccttct      420

gctagc                                                                 426


<210>  32
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  32

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser 
    130                 135                 140         


<210>  33
<211>  2124
<212>  DNA
<213>  Homo sapiens

<400>  33
atgagcctct ggcagcccct ggtcctggtg ctcctggtgc tgggctgctg ctttgctgcc       60

cccagacagc gccagtccac ccttgtgctc ttccctggag acctgagaac caatctcacc      120

gacaggcagc tggcagagga atacctgtac cgctatggtt acactcgggt ggcagagatg      180

cgtggagagt cgaaatctct ggggcctgcg ctgctgcttc tccagaagca actgtccctg      240

cccgagaccg gtgagctgga tagcgccacg ctgaaggcca tgcgaacccc acggtgcggg      300

gtcccagacc tgggcagatt ccaaaccttt gagggcgacc tcaagtggca ccaccacaac      360

atcacctatt ggatccaaaa ctactcggaa gacttgccgc gggcggtgat tgacgacgcc      420

tttgcccgcg ccttcgcact gtggagcgcg gtgacgccgc tcaccttcac tcgcgtgtac      480

agccgggacg cagacatcgt catccagttt ggtgtcgcgg agcacggaga cgggtatccc      540

ttcgacggga aggacgggct cctggcacac gcctttcctc ctggccccgg cattcaggga      600

gacgcccatt tcgacgatga cgagttgtgg tccctgggca agggcgtcgt ggttccaact      660

cggtttggaa acgcagatgg cgcggcctgc cacttcccct tcatcttcga gggccgctcc      720

tactctgcct gcaccaccga cggtcgctcc gacggcttgc cctggtgcag taccacggcc      780

aactacgaca ccgacgaccg gtttggcttc tgccccagcg agagactcta cacccgggac      840

ggcaatgctg atgggaaacc ctgccagttt ccattcatct tccaaggcca atcctactcc      900

gcctgcacca cggacggtcg ctccgacggc taccgctggt gcgccaccac cgccaactac      960

gaccgggaca agctcttcgg cttctgcccg acccgagctg actcgacggt gatggggggc     1020

aactcggcgg gggagctgtg cgtcttcccc ttcactttcc tgggtaagga gtactcgacc     1080

tgtaccagcg agggccgcgg agatgggcgc ctctggtgcg ctaccacctc gaactttgac     1140

agcgacaaga agtggggctt ctgcccggac caaggataca gtttgttcct cgtggcggcg     1200

catgagttcg gccacgcgct gggcttagat cattcctcag tgccggaggc gctcatgtac     1260

cctatgtacc gcttcactga ggggcccccc ttgcataagg acgacgtgaa tggcatccgg     1320

cacctctatg gtcctcgccc tgaacctgag ccacggcctc caaccaccac cacaccgcag     1380

cccacggctc ccccgacggt ctgccccacc ggacccccca ctgtccaccc ctcagagcgc     1440

cccacagctg gccccacagg tcccccctca gctggcccca caggtccccc cactgctggc     1500

ccttctacgg ccactactgt gcctttgagt ccggtggacg atgcctgcaa cgtgaacatc     1560

ttcgacgcca tcgcggagat tgggaaccag ctgtatttgt tcaaggatgg gaagtactgg     1620

cgattctctg agggcagggg gagccggccg cagggcccct tccttatcgc cgacaagtgg     1680

cccgcgctgc cccgcaagct ggactcggtc tttgaggagc cgctctccaa gaagcttttc     1740

ttcttctctg ggcgccaggt gtgggtgtac acaggcgcgt cggtgctggg cccgaggcgt     1800

ctggacaagc tgggcctggg agccgacgtg gcccaggtga ccggggccct ccggagtggc     1860

agggggaaga tgctgctgtt cagcgggcgg cgcctctgga ggttcgacgt gaaggcgcag     1920

atggtggatc cccggagcgc cagcgaggtg gaccggatgt tccccggggt gcctttggac     1980

acgcacgacg tcttccagta ccgagagaaa gcctatttct gccaggaccg cttctactgg     2040

cgcgtgagtt cccggagtga gttgaaccag gtggaccaag tgggctacgt gacctatgac     2100

atcctgcagt gccctgagga ctag                                            2124


<210>  34
<211>  707
<212>  PRT
<213>  Homo sapiens

<400>  34

Met Ser Leu Trp Gln Pro Leu Val Leu Val Leu Leu Val Leu Gly Cys 
1               5                   10                  15      


Cys Phe Ala Ala Pro Arg Gln Arg Gln Ser Thr Leu Val Leu Phe Pro 
            20                  25                  30          


Gly Asp Leu Arg Thr Asn Leu Thr Asp Arg Gln Leu Ala Glu Glu Tyr 
        35                  40                  45              


Leu Tyr Arg Tyr Gly Tyr Thr Arg Val Ala Glu Met Arg Gly Glu Ser 
    50                  55                  60                  


Lys Ser Leu Gly Pro Ala Leu Leu Leu Leu Gln Lys Gln Leu Ser Leu 
65                  70                  75                  80  


Pro Glu Thr Gly Glu Leu Asp Ser Ala Thr Leu Lys Ala Met Arg Thr 
                85                  90                  95      


Pro Arg Cys Gly Val Pro Asp Leu Gly Arg Phe Gln Thr Phe Glu Gly 
            100                 105                 110         


Asp Leu Lys Trp His His His Asn Ile Thr Tyr Trp Ile Gln Asn Tyr 
        115                 120                 125             


Ser Glu Asp Leu Pro Arg Ala Val Ile Asp Asp Ala Phe Ala Arg Ala 
    130                 135                 140                 


Phe Ala Leu Trp Ser Ala Val Thr Pro Leu Thr Phe Thr Arg Val Tyr 
145                 150                 155                 160 


Ser Arg Asp Ala Asp Ile Val Ile Gln Phe Gly Val Ala Glu His Gly 
                165                 170                 175     


Asp Gly Tyr Pro Phe Asp Gly Lys Asp Gly Leu Leu Ala His Ala Phe 
            180                 185                 190         


Pro Pro Gly Pro Gly Ile Gln Gly Asp Ala His Phe Asp Asp Asp Glu 
        195                 200                 205             


Leu Trp Ser Leu Gly Lys Gly Val Val Val Pro Thr Arg Phe Gly Asn 
    210                 215                 220                 


Ala Asp Gly Ala Ala Cys His Phe Pro Phe Ile Phe Glu Gly Arg Ser 
225                 230                 235                 240 


Tyr Ser Ala Cys Thr Thr Asp Gly Arg Ser Asp Gly Leu Pro Trp Cys 
                245                 250                 255     


Ser Thr Thr Ala Asn Tyr Asp Thr Asp Asp Arg Phe Gly Phe Cys Pro 
            260                 265                 270         


Ser Glu Arg Leu Tyr Thr Arg Asp Gly Asn Ala Asp Gly Lys Pro Cys 
        275                 280                 285             


Gln Phe Pro Phe Ile Phe Gln Gly Gln Ser Tyr Ser Ala Cys Thr Thr 
    290                 295                 300                 


Asp Gly Arg Ser Asp Gly Tyr Arg Trp Cys Ala Thr Thr Ala Asn Tyr 
305                 310                 315                 320 


Asp Arg Asp Lys Leu Phe Gly Phe Cys Pro Thr Arg Ala Asp Ser Thr 
                325                 330                 335     


Val Met Gly Gly Asn Ser Ala Gly Glu Leu Cys Val Phe Pro Phe Thr 
            340                 345                 350         


Phe Leu Gly Lys Glu Tyr Ser Thr Cys Thr Ser Glu Gly Arg Gly Asp 
        355                 360                 365             


Gly Arg Leu Trp Cys Ala Thr Thr Ser Asn Phe Asp Ser Asp Lys Lys 
    370                 375                 380                 


Trp Gly Phe Cys Pro Asp Gln Gly Tyr Ser Leu Phe Leu Val Ala Ala 
385                 390                 395                 400 


His Glu Phe Gly His Ala Leu Gly Leu Asp His Ser Ser Val Pro Glu 
                405                 410                 415     


Ala Leu Met Tyr Pro Met Tyr Arg Phe Thr Glu Gly Pro Pro Leu His 
            420                 425                 430         


Lys Asp Asp Val Asn Gly Ile Arg His Leu Tyr Gly Pro Arg Pro Glu 
        435                 440                 445             


Pro Glu Pro Arg Pro Pro Thr Thr Thr Thr Pro Gln Pro Thr Ala Pro 
    450                 455                 460                 


Pro Thr Val Cys Pro Thr Gly Pro Pro Thr Val His Pro Ser Glu Arg 
465                 470                 475                 480 


Pro Thr Ala Gly Pro Thr Gly Pro Pro Ser Ala Gly Pro Thr Gly Pro 
                485                 490                 495     


Pro Thr Ala Gly Pro Ser Thr Ala Thr Thr Val Pro Leu Ser Pro Val 
            500                 505                 510         


Asp Asp Ala Cys Asn Val Asn Ile Phe Asp Ala Ile Ala Glu Ile Gly 
        515                 520                 525             


Asn Gln Leu Tyr Leu Phe Lys Asp Gly Lys Tyr Trp Arg Phe Ser Glu 
    530                 535                 540                 


Gly Arg Gly Ser Arg Pro Gln Gly Pro Phe Leu Ile Ala Asp Lys Trp 
545                 550                 555                 560 


Pro Ala Leu Pro Arg Lys Leu Asp Ser Val Phe Glu Glu Pro Leu Ser 
                565                 570                 575     


Lys Lys Leu Phe Phe Phe Ser Gly Arg Gln Val Trp Val Tyr Thr Gly 
            580                 585                 590         


Ala Ser Val Leu Gly Pro Arg Arg Leu Asp Lys Leu Gly Leu Gly Ala 
        595                 600                 605             


Asp Val Ala Gln Val Thr Gly Ala Leu Arg Ser Gly Arg Gly Lys Met 
    610                 615                 620                 


Leu Leu Phe Ser Gly Arg Arg Leu Trp Arg Phe Asp Val Lys Ala Gln 
625                 630                 635                 640 


Met Val Asp Pro Arg Ser Ala Ser Glu Val Asp Arg Met Phe Pro Gly 
                645                 650                 655     


Val Pro Leu Asp Thr His Asp Val Phe Gln Tyr Arg Glu Lys Ala Tyr 
            660                 665                 670         


Phe Cys Gln Asp Arg Phe Tyr Trp Arg Val Ser Ser Arg Ser Glu Leu 
        675                 680                 685             


Asn Gln Val Asp Gln Val Gly Tyr Val Thr Tyr Asp Ile Leu Gln Cys 
    690                 695                 700                 


Pro Glu Asp 
705         


<210>  35
<211>  324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding PD-L1 light chain Fv

<400>  35
gatatccaga tgacacagag cccatcatct ctgtctgcaa gcgtaggaga ccgagtcacc       60

attacatgca gagcctccca agacgtttcc acagcagtgg cctggtatca gcaaaaacct      120

ggtaaggcgc ccaagcttct catctattca gccagttttc tgtatagcgg cgttcccagc      180

cgattctctg gctctggatc cggcacggac tttactttga caatttcctc tcttcagccc      240

gaagattttg caacctacta ctgtcagcaa tatctctacc atccagccac attcggacag      300

ggcaccaaag tcgaaatcaa aaga                                             324


<210>  36
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-L1 light chain Fv sequence

<400>  36

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             


<210>  37
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding PD-L1 heavy chain Fv

<400>  37
gaagtgcaac tcgttgaaag cggaggaggg cttgtccaac ctggcgggtc actgcggttg       60

agctgcgccg caagcggatt caccttctca gactcttgga tccattgggt gcgccaggct      120

cccggaaaag gcttggaatg ggttgcttgg atttcaccgt atggcggttc cacatactac      180

gctgacagcg ttaagggtcg attcaccatc tctgcagata cttcaaaaaa cacagcctac      240

cttcagatga atagtttgcg cgccgaggac acagcggttt attattgtgc ccgaagacat      300

tggcccggcg gtttcgacta ctgggggcaa ggtacgttgg tgactgtgag cgcc            354


<210>  38
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized PD-L1 heavy chain Fv sequence

<400>  38

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala 
        115             


<210>  39
<211>  699
<212>  DNA
<213>  Homo sapiens

<400>  39
gtagatgaag caaaatcttg tgacaaaacc catacctgcc caccatgccc agccccagaa       60

cttcttggcg gaccctctgt cttccttttc cctccgaagc ccaaggatac cctgatgatc      120

agccgaaccc cggaggtaac atgtgtggtg gtcgatgtta gccatgagga tcctgaagtc      180

aaatttaact ggtatgtaga cggtgttgag gtgcacaacg ctaaaactaa gcccagggag      240

gagcagtaca actcaaccta tcgcgtcgta tctgtgctta ccgtcctgca tcaagactgg      300

ctcaatggta aggaatataa atgtaaagtg agtaacaagg cactgccagc acctatcgaa      360

aaaaccatct caaaggcgaa gggacagccc agggaacccc aggtctatac tctgccacct      420

tctcgggatg aattgaccaa gaaccaagtt agcctgacat gtctggtgaa aggtttctat      480

ccaagcgata tagctgtcga gtgggagtcc aatggccaac ctgagaacaa ttataagacc      540

accccacccg ttctggacag cgacggatcc tttttcctgt actcaaaact cactgtcgat      600

aaatcaagat ggcaacaagg caacgttttt agctgtagcg tgatgcacga agcacttcat      660

aatcactata cacagaagtc actctctctt tctccagga                             699


<210>  40
<211>  233
<212>  PRT
<213>  Homo sapiens

<400>  40

Val Asp Glu Ala Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
1               5                   10                  15      


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            20                  25                  30          


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        35                  40                  45              


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    50                  55                  60                  


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
65                  70                  75                  80  


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                85                  90                  95      


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            100                 105                 110         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        115                 120                 125             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
    130                 135                 140                 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
145                 150                 155                 160 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                165                 170                 175     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            180                 185                 190         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        195                 200                 205             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    210                 215                 220                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
225                 230             


<210>  41
<211>  69
<212>  DNA
<213>  Homo sapiens

<400>  41
gtagatgaag caaaatcttg tgacaaaacc catacctgcc caccatgccc agccccagaa       60

cttcttggc                                                               69


<210>  42
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  42

Val Asp Glu Ala Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
1               5                   10                  15      


Pro Ala Pro Glu Leu Leu Gly 
            20              


<210>  43
<211>  1572
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding CD19-CD3 bi-specific T-cell 
       engager construct

<400>  43
atggagttcg gcctgagctg ggtgttcctg gtggccctgt tcaggggcgt gcagtgcgac       60

atccagctga cccagagccc cgccagcctg gccgtgagcc tgggccagag ggccaccatc      120

agctgcaagg ccagccagag cgtggactac gacggcgaca gctacctgaa ctggtaccag      180

cagatccccg gccagccccc caagctgctg atctacgacg ccagcaacct ggtgagcggc      240

atccccccca ggttcagcgg cagcggcagc ggcaccgact tcaccctgaa catccacccc      300

gtggagaagg tggacgccgc cacctaccac tgccagcaga gcaccgagga cccctggacc      360

ttcggcggcg gcaccaagct ggagatcaag ggcggcggcg gcagcggcgg cggcggcagc      420

ggcggcggcg gcagccaggt gcagctgcag cagagcggcg ccgagctggt gaggcccggc      480

agcagcgtga agatcagctg caaggccagc ggctacgcct tcagcagcta ctggatgaac      540

tgggtgaagc agaggcccgg ccagggcctg gagtggatcg gccagatctg gcccggcgac      600

ggcgacacca actacaacgg caagttcaag ggcaaggcca ccctgaccgc cgacgagagc      660

agcagcaccg cctacatgca gctgagcagc ctggccagcg aggacagcgc cgtgtacttc      720

tgcgccagga gggagaccac caccgtgggc aggtactact acgccatgga ctactggggc      780

cagggcacca ccgtgaccgt gagcagcggc ggcggcggca gcgacatcaa gctgcagcag      840

agcggcgccg agctggccag gcccggcgcc agcgtgaaga tgagctgcaa gaccagcggc      900

tacaccttca ccaggtacac catgcactgg gtgaagcaga ggcccggcca gggcctggag      960

tggatcggct acatcaaccc cagcaggggc tacaccaact acaaccagaa gttcaaggac     1020

aaggccaccc tgaccaccga caagagcagc agcaccgcct acatgcagct gagcagcctg     1080

accagcgagg acagcgccgt gtactactgc gccaggtact acgacgacca ctactgcctg     1140

gactactggg gccagggcac caccctgacc gtgagcagcg tggagggcgg cagcggcggc     1200

agcggcggca gcggcggcag cggcggcgtg gacgacatcc agctgaccca gagccccgcc     1260

atcatgagcg ccagccccgg cgagaaggtg accatgacct gcagggccag cagcagcgtg     1320

agctacatga actggtacca gcagaagagc ggcaccagcc ccaagaggtg gatctacgac     1380

accagcaagg tggccagcgg cgtgccctac aggttcagcg gcagcggcag cggcaccagc     1440

tacagcctga ccatcagcag catggaggcc gaggacgccg ccacctacta ctgccagcag     1500

tggagcagca accccctgac cttcggcgcc ggcaccaagc tggagctgaa gcaccaccac     1560

caccaccact ag                                                         1572


<210>  44
<211>  523
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized CD19-CD3 bi-specific T-cell engager construct

<400>  44

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Tyr Asp Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly 
65                  70                  75                  80  


Ile Pro Pro Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln 
            100                 105                 110         


Gln Ser Thr Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly 
145                 150                 155                 160 


Ser Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser 
                165                 170                 175     


Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
            180                 185                 190         


Ile Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys 
        195                 200                 205             


Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala 
    210                 215                 220                 


Tyr Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe 
225                 230                 235                 240 


Cys Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met 
                245                 250                 255     


Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro 
        275                 280                 285             


Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr 
    290                 295                 300                 


Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
305                 310                 315                 320 


Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln 
                325                 330                 335     


Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr 
            340                 345                 350         


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
        355                 360                 365             


Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly 
    370                 375                 380                 


Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly 
385                 390                 395                 400 


Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr 
                405                 410                 415     


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
            420                 425                 430         


Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln 
        435                 440                 445             


Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val 
    450                 455                 460                 


Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser 
465                 470                 475                 480 


Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr 
                485                 490                 495     


Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr 
            500                 505                 510         


Lys Leu Glu Leu Lys His His His His His His 
        515                 520             


<210>  45
<211>  1177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding SIRP1alpha-CD3-SL bi-specific
       T-cell engager construct

<400>  45
atggagaccg ataccctgct cttgtgggtt ttgcttcttt gggtgccagg atctacaggt       60

gatgaagaag aattgcagat catccaacca gacaaatccg tactcgtggc cgcaggagag      120

accgctaccc tcagatgtac catcacttct ctcttccccg ttggccccat ccagtggttt      180

cgaggcgcag gaccaggacg agtgcttatt tacaatcaac gacagggccc attcccaaga      240

gtgacaacag tatccgatac caccaagcgc aataatatgg actttagcat tagaatcggc      300

aacataacac ccgctgacgc cggtacatac tattgtatta aatttcgaaa gggctcacca      360

gacgacgtgg aatttaagtc aggggccgga accgaactct cagttagagc aaaaccttct      420

gctagcgaca tcaagctgca gcagagcggc gccgagctgg ccaggcccgg cgccagcgtg      480

aagatgagct gcaagaccag cggctacacc ttcaccaggt acaccatgca ctgggtgaag      540

cagaggcccg gccagggcct ggagtggatc ggctacatca accccagcag gggctacacc      600

aactacaacc agaagttcaa ggacaaggcc accctgacca ccgacaagag cagcagcacc      660

gcctacatgc agctgagcag cctgaccagc gaggacagcg ccgtgtacta ctgcgccagg      720

tactacgacg accactactg cctggactac tggggccagg gcaccaccct gaccgtgagc      780

agcgtggagg gcggcagcgg cggcagcggc ggcagcggcg gcagcggcgg cgtggacgac      840

atccagctga cccagagccc cgccatcatg agcgccagcc ccggcgagaa ggtgaccatg      900

acctgcaggg ccagcagcag cgtgagctac atgaactggt accagcagaa gagcggcacc      960

agccccaaga ggtggatcta cgacaccagc aaggtggcca gcggcgtgcc ctacaggttc     1020

agcggcagcg gcagcggcac cagctacagc ctgaccatca gcagcatgga ggccgaggac     1080

gccgccacct actactgcca gcagtggagc agcaaccccc tgaccttcgg cgccggcacc     1140

aagctggagc tgaagcacca ccatcatcac cactgag                              1177


<210>  46
<211>  391
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-CD3-SL bi-specific T-cell engager 
       construct

<400>  46

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Asp Ile 
    130                 135                 140                 


Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val 
145                 150                 155                 160 


Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met 
                165                 170                 175     


His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr 
            180                 185                 190         


Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp 
        195                 200                 205             


Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 
    210                 215                 220                 


Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg 
225                 230                 235                 240 


Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
                245                 250                 255     


Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly Gly Ser 
            260                 265                 270         


Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser Pro Ala 
        275                 280                 285             


Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala 
    290                 295                 300                 


Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr 
305                 310                 315                 320 


Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser Gly Val 
                325                 330                 335     


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr 
            340                 345                 350         


Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
        355                 360                 365             


Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
    370                 375                 380                 


Lys His His His His His His 
385                 390     


<210>  47
<211>  1191
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding SIRP1alpha-CD3-LL bi-specific
       T-cell engager construct

<400>  47
atggagaccg ataccctgct cttgtgggtt ttgcttcttt gggtgccagg atctacaggt       60

gatgaagaag aattgcagat catccaacca gacaaatccg tactcgtggc cgcaggagag      120

accgctaccc tcagatgtac catcacttct ctcttccccg ttggccccat ccagtggttt      180

cgaggcgcag gaccaggacg agtgcttatt tacaatcaac gacagggccc attcccaaga      240

gtgacaacag tatccgatac caccaagcgc aataatatgg actttagcat tagaatcggc      300

aacataacac ccgctgacgc cggtacatac tattgtatta aatttcgaaa gggctcacca      360

gacgacgtgg aatttaagtc aggggccgga accgaactct cagttagagc aaaaccttct      420

gctagcggcg gcggcggcag cgacatcaag ctgcagcaga gcggcgccga gctggccagg      480

cccggcgcca gcgtgaagat gagctgcaag accagcggct acaccttcac caggtacacc      540

atgcactggg tgaagcagag gcccggccag ggcctggagt ggatcggcta catcaacccc      600

agcaggggct acaccaacta caaccagaag ttcaaggaca aggccaccct gaccaccgac      660

aagagcagca gcaccgccta catgcagctg agcagcctga ccagcgagga cagcgccgtg      720

tactactgcg ccaggtacta cgacgaccac tactgcctgg actactgggg ccagggcacc      780

accctgaccg tgagcagcgt ggagggcggc agcggcggca gcggcggcag cggcggcagc      840

ggcggcgtgg acgacatcca gctgacccag agccccgcca tcatgagcgc cagccccggc      900

gagaaggtga ccatgacctg cagggccagc agcagcgtga gctacatgaa ctggtaccag      960

cagaagagcg gcaccagccc caagaggtgg atctacgaca ccagcaaggt ggccagcggc     1020

gtgccctaca ggttcagcgg cagcggcagc ggcaccagct acagcctgac catcagcagc     1080

atggaggccg aggacgccgc cacctactac tgccagcagt ggagcagcaa ccccctgacc     1140

ttcggcgccg gcaccaagct ggagctgaag caccaccacc accaccacta g              1191


<210>  48
<211>  396
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-CD3-LL bi-specific T-cell engager 
       construct

<400>  48

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
145                 150                 155                 160 


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
            180                 185                 190         


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
        195                 200                 205             


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
    210                 215                 220                 


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
                245                 250                 255     


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
            260                 265                 270         


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
        275                 280                 285             


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
    290                 295                 300                 


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
305                 310                 315                 320 


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
                325                 330                 335     


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            340                 345                 350         


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
        355                 360                 365             


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
    370                 375                 380                 


Thr Lys Leu Glu Leu Lys His His His His His His 
385                 390                 395     


<210>  49
<211>  1545
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding PDL1-CD3 bi-specific T-cell 
       engager construct

<400>  49
atggagttcg gcctgagctg ggtgttcctg gtggccctgt tcaggggcgt gcagtgcgac       60

atcaagctgc agcagagcgg cgccgagctg gccaggcccg gcgccagcgt gaagatgagc      120

tgcaagacca gcggctacac cttcaccagg tacaccatgc actgggtgaa gcagaggccc      180

ggccagggcc tggagtggat cggctacatc aaccccagca ggggctacac caactacaac      240

cagaagttca aggacaaggc caccctgacc accgacaaga gcagcagcac cgcctacatg      300

cagctgagca gcctgaccag cgaggacagc gccgtgtact actgcgccag gtactacgac      360

gaccactact gcctggacta ctggggccag ggcaccaccc tgaccgtgag cagcgtggag      420

ggcggcagcg gcggcagcgg cggcagcggc ggcagcggcg gcgtggacga catccagctg      480

acccagagcc ccgccatcat gagcgccagc cccggcgaga aggtgaccat gacctgcagg      540

gccagcagca gcgtgagcta catgaactgg taccagcaga agagcggcac cagccccaag      600

aggtggatct acgacaccag caaggtggcc agcggcgtgc cctacaggtt cagcggcagc      660

ggcagcggca ccagctacag cctgaccatc agcagcatgg aggccgagga cgccgccacc      720

tactactgcc agcagtggag cagcaacccc ctgaccttcg gcgccggcac caagctggag      780

ctgaagggcg gcggcggcag cgatatccag atgacacaga gcccatcatc tctgtctgca      840

agcgtaggag accgagtcac cattacatgc agagcctccc aagacgtttc cacagcagtg      900

gcctggtatc agcaaaaacc tggtaaggcg cccaagcttc tcatctattc agccagtttt      960

ctgtatagcg gcgttcccag ccgattctct ggctctggat ccggcacgga ctttactttg     1020

acaatttcct ctcttcagcc cgaagatttt gcaacctact actgtcagca atatctctac     1080

catccagcca cattcggaca gggcaccaaa gtcgaaatca aaagaggcgg cggcggcagt     1140

ggcggcgggg gttcaggagg cgggggttct gaagtgcaac tcgttgaaag cggaggaggg     1200

cttgtccaac ctggcgggtc actgcggttg agctgcgccg caagcggatt caccttctca     1260

gactcttgga tccattgggt gcgccaggct cccggaaaag gcttggaatg ggttgcttgg     1320

atttcaccgt atggcggttc cacatactac gctgacagcg ttaagggtcg attcaccatc     1380

tctgcagata cttcaaaaaa cacagcctac cttcagatga atagtttgcg cgccgaggac     1440

acagcggttt attattgtgc ccgaagacat tggcccggcg gtttcgacta ctgggggcaa     1500

ggtacgttgg tgactgtgag cgcccaccac catcatcacc actga                     1545


<210>  50
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized PDL1-CD3 bi-specific T-cell engager construct

<400>  50

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
    130                 135                 140                 


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
145                 150                 155                 160 


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
                165                 170                 175     


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
            180                 185                 190         


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
        195                 200                 205             


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
    210                 215                 220                 


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
                245                 250                 255     


Thr Lys Leu Glu Leu Lys Gly Gly Gly Gly Ser Asp Ile Gln Met Thr 
            260                 265                 270         


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
        275                 280                 285             


Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln 
    290                 295                 300                 


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe 
305                 310                 315                 320 


Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                325                 330                 335     


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
            340                 345                 350         


Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly 
        355                 360                 365             


Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    370                 375                 380                 


Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
385                 390                 395                 400 


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
                405                 410                 415     


Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly 
            420                 425                 430         


Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr 
        435                 440                 445             


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr 
    450                 455                 460                 


Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
465                 470                 475                 480 


Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp 
                485                 490                 495     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala His His His His 
            500                 505                 510         


His His 
        


<210>  51
<211>  2244
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding PDL1-CD3-Fc bi-specific 
       T-cell engager construct

<400>  51
atggagttcg gcctgagctg ggtgttcctg gtggccctgt tcaggggcgt gcagtgcgac       60

atcaagctgc agcagagcgg cgccgagctg gccaggcccg gcgccagcgt gaagatgagc      120

tgcaagacca gcggctacac cttcaccagg tacaccatgc actgggtgaa gcagaggccc      180

ggccagggcc tggagtggat cggctacatc aaccccagca ggggctacac caactacaac      240

cagaagttca aggacaaggc caccctgacc accgacaaga gcagcagcac cgcctacatg      300

cagctgagca gcctgaccag cgaggacagc gccgtgtact actgcgccag gtactacgac      360

gaccactact gcctggacta ctggggccag ggcaccaccc tgaccgtgag cagcgtggag      420

ggcggcagcg gcggcagcgg cggcagcggc ggcagcggcg gcgtggacga catccagctg      480

acccagagcc ccgccatcat gagcgccagc cccggcgaga aggtgaccat gacctgcagg      540

gccagcagca gcgtgagcta catgaactgg taccagcaga agagcggcac cagccccaag      600

aggtggatct acgacaccag caaggtggcc agcggcgtgc cctacaggtt cagcggcagc      660

ggcagcggca ccagctacag cctgaccatc agcagcatgg aggccgagga cgccgccacc      720

tactactgcc agcagtggag cagcaacccc ctgaccttcg gcgccggcac caagctggag      780

ctgaagggcg gcggcggcag cgatatccag atgacacaga gcccatcatc tctgtctgca      840

agcgtaggag accgagtcac cattacatgc agagcctccc aagacgtttc cacagcagtg      900

gcctggtatc agcaaaaacc tggtaaggcg cccaagcttc tcatctattc agccagtttt      960

ctgtatagcg gcgttcccag ccgattctct ggctctggat ccggcacgga ctttactttg     1020

acaatttcct ctcttcagcc cgaagatttt gcaacctact actgtcagca atatctctac     1080

catccagcca cattcggaca gggcaccaaa gtcgaaatca aaagaggcgg cggcggcagt     1140

ggcggcgggg gttcaggagg cgggggttct gaagtgcaac tcgttgaaag cggaggaggg     1200

cttgtccaac ctggcgggtc actgcggttg agctgcgccg caagcggatt caccttctca     1260

gactcttgga tccattgggt gcgccaggct cccggaaaag gcttggaatg ggttgcttgg     1320

atttcaccgt atggcggttc cacatactac gctgacagcg ttaagggtcg attcaccatc     1380

tctgcagata cttcaaaaaa cacagcctac cttcagatga atagtttgcg cgccgaggac     1440

acagcggttt attattgtgc ccgaagacat tggcccggcg gtttcgacta ctgggggcaa     1500

ggtacgttgg tgactgtgag cgccgtagat gaagcaaaat cttgtgacaa aacccatacc     1560

tgcccaccat gcccagcccc agaacttctt ggcggaccct ctgtcttcct tttccctccg     1620

aagcccaagg ataccctgat gatcagccga accccggagg taacatgtgt ggtggtcgat     1680

gttagccatg aggatcctga agtcaaattt aactggtatg tagacggtgt tgaggtgcac     1740

aacgctaaaa ctaagcccag ggaggagcag tacaactcaa cctatcgcgt cgtatctgtg     1800

cttaccgtcc tgcatcaaga ctggctcaat ggtaaggaat ataaatgtaa agtgagtaac     1860

aaggcactgc cagcacctat cgaaaaaacc atctcaaagg cgaagggaca gcccagggaa     1920

ccccaggtct atactctgcc accttctcgg gatgaattga ccaagaacca agttagcctg     1980

acatgtctgg tgaaaggttt ctatccaagc gatatagctg tcgagtggga gtccaatggc     2040

caacctgaga acaattataa gaccacccca cccgttctgg acagcgacgg atcctttttc     2100

ctgtactcaa aactcactgt cgataaatca agatggcaac aaggcaacgt ttttagctgt     2160

agcgtgatgc acgaagcact tcataatcac tatacacaga agtcactctc tctttctcca     2220

ggacaccacc atcatcacca ctga                                            2244


<210>  52
<211>  747
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized PDL1-CD3-Fc bi-specific T-cell engager construct

<400>  52

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
    130                 135                 140                 


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
145                 150                 155                 160 


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
                165                 170                 175     


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
            180                 185                 190         


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
        195                 200                 205             


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
    210                 215                 220                 


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
                245                 250                 255     


Thr Lys Leu Glu Leu Lys Gly Gly Gly Gly Ser Asp Ile Gln Met Thr 
            260                 265                 270         


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
        275                 280                 285             


Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln 
    290                 295                 300                 


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe 
305                 310                 315                 320 


Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                325                 330                 335     


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
            340                 345                 350         


Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly 
        355                 360                 365             


Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    370                 375                 380                 


Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
385                 390                 395                 400 


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
                405                 410                 415     


Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly 
            420                 425                 430         


Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr 
        435                 440                 445             


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr 
    450                 455                 460                 


Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
465                 470                 475                 480 


Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp 
                485                 490                 495     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Val Asp Glu Ala 
            500                 505                 510         


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
        515                 520                 525             


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
    530                 535                 540                 


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
545                 550                 555                 560 


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
                565                 570                 575     


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
            580                 585                 590         


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
        595                 600                 605             


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
    610                 615                 620                 


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
625                 630                 635                 640 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
                645                 650                 655     


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
            660                 665                 670         


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
        675                 680                 685             


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
    690                 695                 700                 


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
705                 710                 715                 720 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
                725                 730                 735     


Ser Leu Ser Pro Gly His His His His His His 
            740                 745         


<210>  53
<211>  707
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized CD19-IL15 bi-specific T-cell engager construct

<400>  53

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Tyr Asp Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly 
65                  70                  75                  80  


Ile Pro Pro Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln 
            100                 105                 110         


Gln Ser Thr Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly 
145                 150                 155                 160 


Ser Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser 
                165                 170                 175     


Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
            180                 185                 190         


Ile Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys 
        195                 200                 205             


Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala 
    210                 215                 220                 


Tyr Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe 
225                 230                 235                 240 


Cys Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met 
                245                 250                 255     


Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro 
        275                 280                 285             


Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr 
    290                 295                 300                 


Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
305                 310                 315                 320 


Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln 
                325                 330                 335     


Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr 
            340                 345                 350         


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
        355                 360                 365             


Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly 
    370                 375                 380                 


Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly 
385                 390                 395                 400 


Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr 
                405                 410                 415     


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
            420                 425                 430         


Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln 
        435                 440                 445             


Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val 
    450                 455                 460                 


Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser 
465                 470                 475                 480 


Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr 
                485                 490                 495     


Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr 
            500                 505                 510         


Lys Leu Glu Leu Lys His His His His His His Arg Arg Lys Arg Glu 
        515                 520                 525             


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
    530                 535                 540                 


Pro Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys 
545                 550                 555                 560 


Tyr Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile 
                565                 570                 575     


His Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu 
            580                 585                 590         


Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu 
        595                 600                 605             


Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val 
    610                 615                 620                 


His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu 
625                 630                 635                 640 


Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val 
                645                 650                 655     


Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn 
            660                 665                 670         


Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn 
        675                 680                 685             


Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile 
    690                 695                 700                 


Asn Thr Ser 
705         


<210>  54
<211>  1149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized CD19-IL12 bi-specific T-cell engager construct

<400>  54

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Tyr Asp Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly 
65                  70                  75                  80  


Ile Pro Pro Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln 
            100                 105                 110         


Gln Ser Thr Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly 
145                 150                 155                 160 


Ser Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser 
                165                 170                 175     


Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
            180                 185                 190         


Ile Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys 
        195                 200                 205             


Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala 
    210                 215                 220                 


Tyr Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe 
225                 230                 235                 240 


Cys Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met 
                245                 250                 255     


Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro 
        275                 280                 285             


Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr 
    290                 295                 300                 


Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
305                 310                 315                 320 


Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln 
                325                 330                 335     


Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr 
            340                 345                 350         


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
        355                 360                 365             


Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly 
    370                 375                 380                 


Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly 
385                 390                 395                 400 


Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr 
                405                 410                 415     


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
            420                 425                 430         


Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln 
        435                 440                 445             


Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val 
    450                 455                 460                 


Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser 
465                 470                 475                 480 


Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr 
                485                 490                 495     


Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr 
            500                 505                 510         


Lys Leu Glu Leu Lys His His His His His His Arg Arg Lys Arg Glu 
        515                 520                 525             


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
    530                 535                 540                 


Pro Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala 
545                 550                 555                 560 


Ala Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys 
                565                 570                 575     


Arg Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu 
            580                 585                 590         


Val Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr 
        595                 600                 605             


Pro Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu 
    610                 615                 620                 


Arg Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe 
625                 630                 635                 640 


Tyr Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp 
                645                 650                 655     


Lys Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn 
            660                 665                 670         


Glu Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser 
        675                 680                 685             


Cys Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser 
    690                 695                 700                 


Ser Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met 
705                 710                 715                 720 


Asn Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln 
                725                 730                 735     


Asn Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn 
            740                 745                 750         


Ser Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr 
        755                 760                 765             


Lys Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg 
    770                 775                 780                 


Ala Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Arg Arg 
785                 790                 795                 800 


Lys Arg Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
                805                 810                 815     


Asn Pro Gly Pro Pro Met Cys His Gln Gln Leu Val Ile Ser Trp Phe 
            820                 825                 830         


Ser Leu Val Phe Leu Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys 
        835                 840                 845             


Lys Asp Val Tyr Val Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly 
    850                 855                 860                 


Glu Met Val Val Leu Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr 
865                 870                 875                 880 


Trp Thr Leu Asp Gln Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu 
                885                 890                 895     


Thr Ile Gln Val Lys Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His 
            900                 905                 910         


Lys Gly Gly Glu Val Leu Ser His Ser Leu Leu Leu Leu His Lys Lys 
        915                 920                 925             


Glu Asp Gly Ile Trp Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro 
    930                 935                 940                 


Lys Asn Lys Thr Phe Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg 
945                 950                 955                 960 


Phe Thr Cys Trp Trp Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser 
                965                 970                 975     


Val Lys Ser Ser Arg Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly 
            980                 985                 990         


Ala Ala Thr Leu Ser Ala Glu Arg  Val Arg Gly Asp Asn  Lys Glu Tyr 
        995                 1000                 1005             


Glu Tyr  Ser Val Glu Cys Gln  Glu Asp Ser Ala Cys  Pro Ala Ala 
    1010                 1015                 1020             


Glu Glu  Ser Leu Pro Ile Glu  Val Met Val Asp Ala  Val His Lys 
    1025                 1030                 1035             


Leu Lys  Tyr Glu Asn Tyr Thr  Ser Ser Phe Phe Ile  Arg Asp Ile 
    1040                 1045                 1050             


Ile Lys  Pro Asp Pro Pro Lys  Asn Leu Gln Leu Lys  Pro Leu Lys 
    1055                 1060                 1065             


Asn Ser  Arg Gln Val Glu Val  Ser Trp Glu Tyr Pro  Asp Thr Trp 
    1070                 1075                 1080             


Ser Thr  Pro His Ser Tyr Phe  Ser Leu Thr Phe Cys  Val Gln Val 
    1085                 1090                 1095             


Gln Gly  Lys Ser Lys Arg Glu  Lys Lys Asp Arg Val  Phe Thr Asp 
    1100                 1105                 1110             


Lys Thr  Ser Ala Thr Val Ile  Cys Arg Lys Asn Ala  Ser Ile Ser 
    1115                 1120                 1125             


Val Arg  Ala Gln Asp Arg Tyr  Tyr Ser Ser Ser Trp  Ser Glu Trp 
    1130                 1135                 1140             


Ala Ser  Val Pro Cys Ser 
    1145                 


<210>  55
<211>  643
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized CD19-CXCL10 bi-specific T-cell engager construct

<400>  55

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Gln Leu Thr Gln Ser Pro Ala Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val 
        35                  40                  45              


Asp Tyr Asp Gly Asp Ser Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly 
    50                  55                  60                  


Gln Pro Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly 
65                  70                  75                  80  


Ile Pro Pro Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
                85                  90                  95      


Asn Ile His Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln 
            100                 105                 110         


Gln Ser Thr Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu 
        115                 120                 125             


Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly 
145                 150                 155                 160 


Ser Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser 
                165                 170                 175     


Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp 
            180                 185                 190         


Ile Gly Gln Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys 
        195                 200                 205             


Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala 
    210                 215                 220                 


Tyr Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Phe 
225                 230                 235                 240 


Cys Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr Tyr Ala Met 
                245                 250                 255     


Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro 
        275                 280                 285             


Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr 
    290                 295                 300                 


Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
305                 310                 315                 320 


Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln 
                325                 330                 335     


Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr 
            340                 345                 350         


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
        355                 360                 365             


Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly 
    370                 375                 380                 


Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly 
385                 390                 395                 400 


Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr 
                405                 410                 415     


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
            420                 425                 430         


Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln 
        435                 440                 445             


Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val 
    450                 455                 460                 


Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser 
465                 470                 475                 480 


Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr 
                485                 490                 495     


Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr 
            500                 505                 510         


Lys Leu Glu Leu Lys His His His His His His Arg Arg Lys Arg Glu 
        515                 520                 525             


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
    530                 535                 540                 


Pro Met Asn Gln Thr Ala Ile Leu Ile Cys Cys Leu Ile Phe Leu Thr 
545                 550                 555                 560 


Leu Ser Gly Ile Gln Gly Val Pro Leu Ser Arg Thr Val Arg Cys Thr 
                565                 570                 575     


Cys Ile Ser Ile Ser Asn Gln Pro Val Asn Pro Arg Ser Leu Glu Lys 
            580                 585                 590         


Leu Glu Ile Ile Pro Ala Ser Gln Phe Cys Pro Arg Val Glu Ile Ile 
        595                 600                 605             


Ala Thr Met Lys Lys Lys Gly Glu Lys Arg Cys Leu Asn Pro Glu Ser 
    610                 615                 620                 


Lys Ala Ile Lys Asn Leu Leu Lys Ala Val Ser Lys Glu Arg Ser Lys 
625                 630                 635                 640 


Arg Ser Pro 
            


<210>  56
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-IL15-SL bi-specific T-cell engager 
       construct

<400>  56

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Asp Ile 
    130                 135                 140                 


Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val 
145                 150                 155                 160 


Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met 
                165                 170                 175     


His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr 
            180                 185                 190         


Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp 
        195                 200                 205             


Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 
    210                 215                 220                 


Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg 
225                 230                 235                 240 


Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
                245                 250                 255     


Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly Gly Ser 
            260                 265                 270         


Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser Pro Ala 
        275                 280                 285             


Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala 
    290                 295                 300                 


Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr 
305                 310                 315                 320 


Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser Gly Val 
                325                 330                 335     


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr 
            340                 345                 350         


Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
        355                 360                 365             


Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
    370                 375                 380                 


Lys His His His His His His Arg Arg Lys Arg Glu Gly Arg Gly Ser 
385                 390                 395                 400 


Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Arg Ile 
                405                 410                 415     


Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr Leu Cys Leu 
            420                 425                 430         


Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His Val Phe Ile 
        435                 440                 445             


Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala Asn Trp Val 
    450                 455                 460                 


Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met 
465                 470                 475                 480 


His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys 
                485                 490                 495     


Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser 
            500                 505                 510         


Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile 
        515                 520                 525             


Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser 
    530                 535                 540                 


Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe 
545                 550                 555                 560 


Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn Thr Ser 
                565                 570                 575 


<210>  57
<211>  580
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-IL15-LL bi-specific T-cell engager 
       construct

<400>  57

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
145                 150                 155                 160 


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
            180                 185                 190         


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
        195                 200                 205             


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
    210                 215                 220                 


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
                245                 250                 255     


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
            260                 265                 270         


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
        275                 280                 285             


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
    290                 295                 300                 


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
305                 310                 315                 320 


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
                325                 330                 335     


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            340                 345                 350         


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
        355                 360                 365             


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
    370                 375                 380                 


Thr Lys Leu Glu Leu Lys His His His His His His Arg Arg Lys Arg 
385                 390                 395                 400 


Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
                405                 410                 415     


Gly Pro Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln 
            420                 425                 430         


Cys Tyr Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly 
        435                 440                 445             


Ile His Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr 
    450                 455                 460                 


Glu Ala Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp 
465                 470                 475                 480 


Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp 
                485                 490                 495     


Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu 
            500                 505                 510         


Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr 
        515                 520                 525             


Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly 
    530                 535                 540                 


Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys 
545                 550                 555                 560 


Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe 
                565                 570                 575     


Ile Asn Thr Ser 
            580 


<210>  58
<211>  1017
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-IL12-SL bi-specific T-cell engager 
       construct

<400>  58

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Asp Ile 
    130                 135                 140                 


Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val 
145                 150                 155                 160 


Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met 
                165                 170                 175     


His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr 
            180                 185                 190         


Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp 
        195                 200                 205             


Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 
    210                 215                 220                 


Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg 
225                 230                 235                 240 


Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
                245                 250                 255     


Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly Gly Ser 
            260                 265                 270         


Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser Pro Ala 
        275                 280                 285             


Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala 
    290                 295                 300                 


Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr 
305                 310                 315                 320 


Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser Gly Val 
                325                 330                 335     


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr 
            340                 345                 350         


Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
        355                 360                 365             


Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
    370                 375                 380                 


Lys His His His His His His Arg Arg Lys Arg Glu Gly Arg Gly Ser 
385                 390                 395                 400 


Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Trp Pro 
                405                 410                 415     


Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala Ala Thr Gly 
            420                 425                 430         


Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg Leu Ser Met 
        435                 440                 445             


Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val Leu Leu Asp 
    450                 455                 460                 


His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro Asp Pro Gly 
465                 470                 475                 480 


Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg Ala Val Ser 
                485                 490                 495     


Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys Thr 
            500                 505                 510         


Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys Thr Ser Thr 
        515                 520                 525             


Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys Leu 
    530                 535                 540                 


Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala Ser 
545                 550                 555                 560 


Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser Ile Tyr Glu 
                565                 570                 575     


Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn Ala Lys Leu 
            580                 585                 590         


Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn Met Leu Ala 
        595                 600                 605             


Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser Glu Thr Val 
    610                 615                 620                 


Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys Ile 
625                 630                 635                 640 


Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala Val Thr Ile 
                645                 650                 655     


Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Arg Arg Lys Arg Glu Gly 
            660                 665                 670         


Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
        675                 680                 685             


Pro Met Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe 
    690                 695                 700                 


Leu Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr 
705                 710                 715                 720 


Val Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val 
                725                 730                 735     


Leu Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp 
            740                 745                 750         


Gln Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val 
        755                 760                 765             


Lys Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu 
    770                 775                 780                 


Val Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile 
785                 790                 795                 800 


Trp Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr 
                805                 810                 815     


Phe Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp 
            820                 825                 830         


Trp Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser 
        835                 840                 845             


Arg Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu 
    850                 855                 860                 


Ser Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val 
865                 870                 875                 880 


Glu Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro 
                885                 890                 895     


Ile Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr 
            900                 905                 910         


Thr Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys 
        915                 920                 925             


Asn Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser 
    930                 935                 940                 


Trp Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu 
945                 950                 955                 960 


Thr Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp 
                965                 970                 975     


Arg Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn 
            980                 985                 990         


Ala Ser Ile Ser Val Arg Ala Gln  Asp Arg Tyr Tyr Ser  Ser Ser Trp 
        995                 1000                 1005             


Ser Glu  Trp Ala Ser Val Pro  Cys Ser 
    1010                 1015         


<210>  59
<211>  1022
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-IL12-LL bi-specific T-cell engager 
       construct

<400>  59

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
145                 150                 155                 160 


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
            180                 185                 190         


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
        195                 200                 205             


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
    210                 215                 220                 


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
                245                 250                 255     


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
            260                 265                 270         


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
        275                 280                 285             


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
    290                 295                 300                 


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
305                 310                 315                 320 


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
                325                 330                 335     


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            340                 345                 350         


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
        355                 360                 365             


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
    370                 375                 380                 


Thr Lys Leu Glu Leu Lys His His His His His His Arg Arg Lys Arg 
385                 390                 395                 400 


Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
                405                 410                 415     


Gly Pro Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro 
            420                 425                 430         


Ala Ala Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln 
        435                 440                 445             


Cys Arg Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr 
    450                 455                 460                 


Leu Val Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala 
465                 470                 475                 480 


Thr Pro Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu 
                485                 490                 495     


Leu Arg Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu 
            500                 505                 510         


Phe Tyr Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys 
        515                 520                 525             


Asp Lys Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys 
    530                 535                 540                 


Asn Glu Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly 
545                 550                 555                 560 


Ser Cys Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu 
                565                 570                 575     


Ser Ser Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr 
            580                 585                 590         


Met Asn Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp 
        595                 600                 605             


Gln Asn Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe 
    610                 615                 620                 


Asn Ser Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe 
625                 630                 635                 640 


Tyr Lys Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile 
                645                 650                 655     


Arg Ala Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Arg 
            660                 665                 670         


Arg Lys Arg Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
        675                 680                 685             


Glu Asn Pro Gly Pro Pro Met Cys His Gln Gln Leu Val Ile Ser Trp 
    690                 695                 700                 


Phe Ser Leu Val Phe Leu Ala Ser Pro Leu Val Ala Ile Trp Glu Leu 
705                 710                 715                 720 


Lys Lys Asp Val Tyr Val Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro 
                725                 730                 735     


Gly Glu Met Val Val Leu Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile 
            740                 745                 750         


Thr Trp Thr Leu Asp Gln Ser Ser Glu Val Leu Gly Ser Gly Lys Thr 
        755                 760                 765             


Leu Thr Ile Gln Val Lys Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys 
    770                 775                 780                 


His Lys Gly Gly Glu Val Leu Ser His Ser Leu Leu Leu Leu His Lys 
785                 790                 795                 800 


Lys Glu Asp Gly Ile Trp Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu 
                805                 810                 815     


Pro Lys Asn Lys Thr Phe Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly 
            820                 825                 830         


Arg Phe Thr Cys Trp Trp Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe 
        835                 840                 845             


Ser Val Lys Ser Ser Arg Gly Ser Ser Asp Pro Gln Gly Val Thr Cys 
    850                 855                 860                 


Gly Ala Ala Thr Leu Ser Ala Glu Arg Val Arg Gly Asp Asn Lys Glu 
865                 870                 875                 880 


Tyr Glu Tyr Ser Val Glu Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala 
                885                 890                 895     


Glu Glu Ser Leu Pro Ile Glu Val Met Val Asp Ala Val His Lys Leu 
            900                 905                 910         


Lys Tyr Glu Asn Tyr Thr Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys 
        915                 920                 925             


Pro Asp Pro Pro Lys Asn Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg 
    930                 935                 940                 


Gln Val Glu Val Ser Trp Glu Tyr Pro Asp Thr Trp Ser Thr Pro His 
945                 950                 955                 960 


Ser Tyr Phe Ser Leu Thr Phe Cys Val Gln Val Gln Gly Lys Ser Lys 
                965                 970                 975     


Arg Glu Lys Lys Asp Arg Val Phe Thr Asp Lys Thr Ser Ala Thr Val 
            980                 985                 990         


Ile Cys Arg Lys Asn Ala Ser Ile  Ser Val Arg Ala Gln  Asp Arg Tyr 
        995                 1000                 1005             


Tyr Ser  Ser Ser Trp Ser Glu  Trp Ala Ser Val Pro  Cys Ser 
    1010                 1015                 1020         


<210>  60
<211>  511
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-CXCL10-SL bi-specific T-cell engager 
       construct

<400>  60

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Asp Ile 
    130                 135                 140                 


Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val 
145                 150                 155                 160 


Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met 
                165                 170                 175     


His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr 
            180                 185                 190         


Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp 
        195                 200                 205             


Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 
    210                 215                 220                 


Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg 
225                 230                 235                 240 


Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
                245                 250                 255     


Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly Gly Ser 
            260                 265                 270         


Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser Pro Ala 
        275                 280                 285             


Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala 
    290                 295                 300                 


Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr 
305                 310                 315                 320 


Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser Gly Val 
                325                 330                 335     


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr 
            340                 345                 350         


Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
        355                 360                 365             


Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
    370                 375                 380                 


Lys His His His His His His Arg Arg Lys Arg Glu Gly Arg Gly Ser 
385                 390                 395                 400 


Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Asn Gln 
                405                 410                 415     


Thr Ala Ile Leu Ile Cys Cys Leu Ile Phe Leu Thr Leu Ser Gly Ile 
            420                 425                 430         


Gln Gly Val Pro Leu Ser Arg Thr Val Arg Cys Thr Cys Ile Ser Ile 
        435                 440                 445             


Ser Asn Gln Pro Val Asn Pro Arg Ser Leu Glu Lys Leu Glu Ile Ile 
    450                 455                 460                 


Pro Ala Ser Gln Phe Cys Pro Arg Val Glu Ile Ile Ala Thr Met Lys 
465                 470                 475                 480 


Lys Lys Gly Glu Lys Arg Cys Leu Asn Pro Glu Ser Lys Ala Ile Lys 
                485                 490                 495     


Asn Leu Leu Lys Ala Val Ser Lys Glu Arg Ser Lys Arg Ser Pro 
            500                 505                 510     


<210>  61
<211>  516
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-CXCL10-LL bi-specific T-cell engager 
       construct

<400>  61

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
145                 150                 155                 160 


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
            180                 185                 190         


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
        195                 200                 205             


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
    210                 215                 220                 


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
                245                 250                 255     


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
            260                 265                 270         


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
        275                 280                 285             


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
    290                 295                 300                 


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
305                 310                 315                 320 


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
                325                 330                 335     


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            340                 345                 350         


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
        355                 360                 365             


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
    370                 375                 380                 


Thr Lys Leu Glu Leu Lys His His His His His His Arg Arg Lys Arg 
385                 390                 395                 400 


Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
                405                 410                 415     


Gly Pro Met Asn Gln Thr Ala Ile Leu Ile Cys Cys Leu Ile Phe Leu 
            420                 425                 430         


Thr Leu Ser Gly Ile Gln Gly Val Pro Leu Ser Arg Thr Val Arg Cys 
        435                 440                 445             


Thr Cys Ile Ser Ile Ser Asn Gln Pro Val Asn Pro Arg Ser Leu Glu 
    450                 455                 460                 


Lys Leu Glu Ile Ile Pro Ala Ser Gln Phe Cys Pro Arg Val Glu Ile 
465                 470                 475                 480 


Ile Ala Thr Met Lys Lys Lys Gly Glu Lys Arg Cys Leu Asn Pro Glu 
                485                 490                 495     


Ser Lys Ala Ile Lys Asn Leu Leu Lys Ala Val Ser Lys Glu Arg Ser 
            500                 505                 510         


Lys Arg Ser Pro 
        515     


<210>  62
<211>  698
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized PDL1-CD3-IL15 bi-specific T-cell engager construct

<400>  62

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
    130                 135                 140                 


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
145                 150                 155                 160 


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
                165                 170                 175     


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
            180                 185                 190         


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
        195                 200                 205             


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
    210                 215                 220                 


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
                245                 250                 255     


Thr Lys Leu Glu Leu Lys Gly Gly Gly Gly Ser Asp Ile Gln Met Thr 
            260                 265                 270         


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
        275                 280                 285             


Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln 
    290                 295                 300                 


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe 
305                 310                 315                 320 


Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                325                 330                 335     


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
            340                 345                 350         


Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly 
        355                 360                 365             


Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    370                 375                 380                 


Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
385                 390                 395                 400 


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
                405                 410                 415     


Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly 
            420                 425                 430         


Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr 
        435                 440                 445             


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr 
    450                 455                 460                 


Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
465                 470                 475                 480 


Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp 
                485                 490                 495     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala His His His His 
            500                 505                 510         


His His Arg Arg Lys Arg Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly 
        515                 520                 525             


Asp Val Glu Glu Asn Pro Gly Pro Met Arg Ile Ser Lys Pro His Leu 
    530                 535                 540                 


Arg Ser Ile Ser Ile Gln Cys Tyr Leu Cys Leu Leu Leu Asn Ser His 
545                 550                 555                 560 


Phe Leu Thr Glu Ala Gly Ile His Val Phe Ile Leu Gly Cys Phe Ser 
                565                 570                 575     


Ala Gly Leu Pro Lys Thr Glu Ala Asn Trp Val Asn Val Ile Ser Asp 
            580                 585                 590         


Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr 
        595                 600                 605             


Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met 
    610                 615                 620                 


Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp 
625                 630                 635                 640 


Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn 
                645                 650                 655     


Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys 
            660                 665                 670         


Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val 
        675                 680                 685             


His Ile Val Gln Met Phe Ile Asn Thr Ser 
    690                 695             


<210>  63
<211>  1140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized PDL1-CD3-IL12 bi-specific T-cell engager construct

<400>  63

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
    130                 135                 140                 


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
145                 150                 155                 160 


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
                165                 170                 175     


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
            180                 185                 190         


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
        195                 200                 205             


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
    210                 215                 220                 


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
                245                 250                 255     


Thr Lys Leu Glu Leu Lys Gly Gly Gly Gly Ser Asp Ile Gln Met Thr 
            260                 265                 270         


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
        275                 280                 285             


Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln 
    290                 295                 300                 


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe 
305                 310                 315                 320 


Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                325                 330                 335     


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
            340                 345                 350         


Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly 
        355                 360                 365             


Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    370                 375                 380                 


Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
385                 390                 395                 400 


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
                405                 410                 415     


Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly 
            420                 425                 430         


Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr 
        435                 440                 445             


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr 
    450                 455                 460                 


Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
465                 470                 475                 480 


Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp 
                485                 490                 495     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala His His His His 
            500                 505                 510         


His His Arg Arg Lys Arg Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly 
        515                 520                 525             


Asp Val Glu Glu Asn Pro Gly Pro Met Trp Pro Pro Gly Ser Ala Ser 
    530                 535                 540                 


Gln Pro Pro Pro Ser Pro Ala Ala Ala Thr Gly Leu His Pro Ala Ala 
545                 550                 555                 560 


Arg Pro Val Ser Leu Gln Cys Arg Leu Ser Met Cys Pro Ala Arg Ser 
                565                 570                 575     


Leu Leu Leu Val Ala Thr Leu Val Leu Leu Asp His Leu Ser Leu Ala 
            580                 585                 590         


Arg Asn Leu Pro Val Ala Thr Pro Asp Pro Gly Met Phe Pro Cys Leu 
        595                 600                 605             


His His Ser Gln Asn Leu Leu Arg Ala Val Ser Asn Met Leu Gln Lys 
    610                 615                 620                 


Ala Arg Gln Thr Leu Glu Phe Tyr Pro Cys Thr Ser Glu Glu Ile Asp 
625                 630                 635                 640 


His Glu Asp Ile Thr Lys Asp Lys Thr Ser Thr Val Glu Ala Cys Leu 
                645                 650                 655     


Pro Leu Glu Leu Thr Lys Asn Glu Ser Cys Leu Asn Ser Arg Glu Thr 
            660                 665                 670         


Ser Phe Ile Thr Asn Gly Ser Cys Leu Ala Ser Arg Lys Thr Ser Phe 
        675                 680                 685             


Met Met Ala Leu Cys Leu Ser Ser Ile Tyr Glu Asp Leu Lys Met Tyr 
    690                 695                 700                 


Gln Val Glu Phe Lys Thr Met Asn Ala Lys Leu Leu Met Asp Pro Lys 
705                 710                 715                 720 


Arg Gln Ile Phe Leu Asp Gln Asn Met Leu Ala Val Ile Asp Glu Leu 
                725                 730                 735     


Met Gln Ala Leu Asn Phe Asn Ser Glu Thr Val Pro Gln Lys Ser Ser 
            740                 745                 750         


Leu Glu Glu Pro Asp Phe Tyr Lys Thr Lys Ile Lys Leu Cys Ile Leu 
        755                 760                 765             


Leu His Ala Phe Arg Ile Arg Ala Val Thr Ile Asp Arg Val Met Ser 
    770                 775                 780                 


Tyr Leu Asn Ala Ser Arg Arg Lys Arg Glu Gly Arg Gly Ser Leu Leu 
785                 790                 795                 800 


Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Pro Met Cys His Gln 
                805                 810                 815     


Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu Ala Ser Pro Leu 
            820                 825                 830         


Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val Val Glu Leu Asp 
        835                 840                 845             


Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu Thr Cys Asp Thr 
    850                 855                 860                 


Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln Ser Ser Glu Val 
865                 870                 875                 880 


Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys Glu Phe Gly Asp 
                885                 890                 895     


Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val Leu Ser His Ser 
            900                 905                 910         


Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp Ser Thr Asp Ile 
        915                 920                 925             


Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe Leu Arg Cys Glu 
    930                 935                 940                 


Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp Leu Thr Thr Ile 
945                 950                 955                 960 


Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg Gly Ser Ser Asp 
                965                 970                 975     


Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser Ala Glu Arg Val 
            980                 985                 990         


Arg Gly Asp Asn Lys Glu Tyr Glu  Tyr Ser Val Glu Cys  Gln Glu Asp 
        995                 1000                 1005             


Ser Ala  Cys Pro Ala Ala Glu  Glu Ser Leu Pro Ile  Glu Val Met 
    1010                 1015                 1020             


Val Asp  Ala Val His Lys Leu  Lys Tyr Glu Asn Tyr  Thr Ser Ser 
    1025                 1030                 1035             


Phe Phe  Ile Arg Asp Ile Ile  Lys Pro Asp Pro Pro  Lys Asn Leu 
    1040                 1045                 1050             


Gln Leu  Lys Pro Leu Lys Asn  Ser Arg Gln Val Glu  Val Ser Trp 
    1055                 1060                 1065             


Glu Tyr  Pro Asp Thr Trp Ser  Thr Pro His Ser Tyr  Phe Ser Leu 
    1070                 1075                 1080             


Thr Phe  Cys Val Gln Val Gln  Gly Lys Ser Lys Arg  Glu Lys Lys 
    1085                 1090                 1095             


Asp Arg  Val Phe Thr Asp Lys  Thr Ser Ala Thr Val  Ile Cys Arg 
    1100                 1105                 1110             


Lys Asn  Ala Ser Ile Ser Val  Arg Ala Gln Asp Arg  Tyr Tyr Ser 
    1115                 1120                 1125             


Ser Ser  Trp Ser Glu Trp Ala  Ser Val Pro Cys Ser  
    1130                 1135                 1140 


<210>  64
<211>  634
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized PDL1-CD3-CXCL10 bi-specific T-cell engager construct

<400>  64

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
    130                 135                 140                 


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
145                 150                 155                 160 


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
                165                 170                 175     


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
            180                 185                 190         


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
        195                 200                 205             


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
    210                 215                 220                 


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
                245                 250                 255     


Thr Lys Leu Glu Leu Lys Gly Gly Gly Gly Ser Asp Ile Gln Met Thr 
            260                 265                 270         


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
        275                 280                 285             


Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln 
    290                 295                 300                 


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe 
305                 310                 315                 320 


Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                325                 330                 335     


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
            340                 345                 350         


Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly 
        355                 360                 365             


Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    370                 375                 380                 


Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
385                 390                 395                 400 


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
                405                 410                 415     


Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly 
            420                 425                 430         


Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr 
        435                 440                 445             


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr 
    450                 455                 460                 


Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
465                 470                 475                 480 


Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp 
                485                 490                 495     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala His His His His 
            500                 505                 510         


His His Arg Arg Lys Arg Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly 
        515                 520                 525             


Asp Val Glu Glu Asn Pro Gly Pro Met Asn Gln Thr Ala Ile Leu Ile 
    530                 535                 540                 


Cys Cys Leu Ile Phe Leu Thr Leu Ser Gly Ile Gln Gly Val Pro Leu 
545                 550                 555                 560 


Ser Arg Thr Val Arg Cys Thr Cys Ile Ser Ile Ser Asn Gln Pro Val 
                565                 570                 575     


Asn Pro Arg Ser Leu Glu Lys Leu Glu Ile Ile Pro Ala Ser Gln Phe 
            580                 585                 590         


Cys Pro Arg Val Glu Ile Ile Ala Thr Met Lys Lys Lys Gly Glu Lys 
        595                 600                 605             


Arg Cys Leu Asn Pro Glu Ser Lys Ala Ile Lys Asn Leu Leu Lys Ala 
    610                 615                 620                 


Val Ser Lys Glu Arg Ser Lys Arg Ser Pro 
625                 630                 


<210>  65
<211>  1120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-MMP9-SL bi-specific T-cell engager 
       construct

<400>  65

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Asp Ile 
    130                 135                 140                 


Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val 
145                 150                 155                 160 


Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr Thr Met 
                165                 170                 175     


His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr 
            180                 185                 190         


Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe Lys Asp 
        195                 200                 205             


Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 
    210                 215                 220                 


Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg 
225                 230                 235                 240 


Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
                245                 250                 255     


Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly Gly Ser 
            260                 265                 270         


Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser Pro Ala 
        275                 280                 285             


Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala 
    290                 295                 300                 


Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser Gly Thr 
305                 310                 315                 320 


Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser Gly Val 
                325                 330                 335     


Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr 
            340                 345                 350         


Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
        355                 360                 365             


Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
    370                 375                 380                 


Lys His His His His His His Arg Arg Lys Arg Glu Gly Arg Gly Ser 
385                 390                 395                 400 


Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ser Leu 
                405                 410                 415     


Trp Gln Pro Leu Val Leu Val Leu Leu Val Leu Gly Cys Cys Phe Ala 
            420                 425                 430         


Ala Pro Arg Gln Arg Gln Ser Thr Leu Val Leu Phe Pro Gly Asp Leu 
        435                 440                 445             


Arg Thr Asn Leu Thr Asp Arg Gln Leu Ala Glu Glu Tyr Leu Tyr Arg 
    450                 455                 460                 


Tyr Gly Tyr Thr Arg Val Ala Glu Met Arg Gly Glu Ser Lys Ser Leu 
465                 470                 475                 480 


Gly Pro Ala Leu Leu Leu Leu Gln Lys Gln Leu Ser Leu Pro Glu Thr 
                485                 490                 495     


Gly Glu Leu Asp Ser Ala Thr Leu Lys Ala Met Arg Thr Pro Arg Cys 
            500                 505                 510         


Gly Val Pro Asp Leu Gly Arg Phe Gln Thr Phe Glu Gly Asp Leu Lys 
        515                 520                 525             


Trp His His His Asn Ile Thr Tyr Trp Ile Gln Asn Tyr Ser Glu Asp 
    530                 535                 540                 


Leu Pro Arg Ala Val Ile Asp Asp Ala Phe Ala Arg Ala Phe Ala Leu 
545                 550                 555                 560 


Trp Ser Ala Val Thr Pro Leu Thr Phe Thr Arg Val Tyr Ser Arg Asp 
                565                 570                 575     


Ala Asp Ile Val Ile Gln Phe Gly Val Ala Glu His Gly Asp Gly Tyr 
            580                 585                 590         


Pro Phe Asp Gly Lys Asp Gly Leu Leu Ala His Ala Phe Pro Pro Gly 
        595                 600                 605             


Pro Gly Ile Gln Gly Asp Ala His Phe Asp Asp Asp Glu Leu Trp Ser 
    610                 615                 620                 


Leu Gly Lys Gly Val Val Val Pro Thr Arg Phe Gly Asn Ala Asp Gly 
625                 630                 635                 640 


Ala Ala Cys His Phe Pro Phe Ile Phe Glu Gly Arg Ser Tyr Ser Ala 
                645                 650                 655     


Cys Thr Thr Asp Gly Arg Ser Asp Gly Leu Pro Trp Cys Ser Thr Thr 
            660                 665                 670         


Ala Asn Tyr Asp Thr Asp Asp Arg Phe Gly Phe Cys Pro Ser Glu Arg 
        675                 680                 685             


Leu Tyr Thr Arg Asp Gly Asn Ala Asp Gly Lys Pro Cys Gln Phe Pro 
    690                 695                 700                 


Phe Ile Phe Gln Gly Gln Ser Tyr Ser Ala Cys Thr Thr Asp Gly Arg 
705                 710                 715                 720 


Ser Asp Gly Tyr Arg Trp Cys Ala Thr Thr Ala Asn Tyr Asp Arg Asp 
                725                 730                 735     


Lys Leu Phe Gly Phe Cys Pro Thr Arg Ala Asp Ser Thr Val Met Gly 
            740                 745                 750         


Gly Asn Ser Ala Gly Glu Leu Cys Val Phe Pro Phe Thr Phe Leu Gly 
        755                 760                 765             


Lys Glu Tyr Ser Thr Cys Thr Ser Glu Gly Arg Gly Asp Gly Arg Leu 
    770                 775                 780                 


Trp Cys Ala Thr Thr Ser Asn Phe Asp Ser Asp Lys Lys Trp Gly Phe 
785                 790                 795                 800 


Cys Pro Asp Gln Gly Tyr Ser Leu Phe Leu Val Ala Ala His Glu Phe 
                805                 810                 815     


Gly His Ala Leu Gly Leu Asp His Ser Ser Val Pro Glu Ala Leu Met 
            820                 825                 830         


Tyr Pro Met Tyr Arg Phe Thr Glu Gly Pro Pro Leu His Lys Asp Asp 
        835                 840                 845             


Val Asn Gly Ile Arg His Leu Tyr Gly Pro Arg Pro Glu Pro Glu Pro 
    850                 855                 860                 


Arg Pro Pro Thr Thr Thr Thr Pro Gln Pro Thr Ala Pro Pro Thr Val 
865                 870                 875                 880 


Cys Pro Thr Gly Pro Pro Thr Val His Pro Ser Glu Arg Pro Thr Ala 
                885                 890                 895     


Gly Pro Thr Gly Pro Pro Ser Ala Gly Pro Thr Gly Pro Pro Thr Ala 
            900                 905                 910         


Gly Pro Ser Thr Ala Thr Thr Val Pro Leu Ser Pro Val Asp Asp Ala 
        915                 920                 925             


Cys Asn Val Asn Ile Phe Asp Ala Ile Ala Glu Ile Gly Asn Gln Leu 
    930                 935                 940                 


Tyr Leu Phe Lys Asp Gly Lys Tyr Trp Arg Phe Ser Glu Gly Arg Gly 
945                 950                 955                 960 


Ser Arg Pro Gln Gly Pro Phe Leu Ile Ala Asp Lys Trp Pro Ala Leu 
                965                 970                 975     


Pro Arg Lys Leu Asp Ser Val Phe Glu Glu Pro Leu Ser Lys Lys Leu 
            980                 985                 990         


Phe Phe Phe Ser Gly Arg Gln Val  Trp Val Tyr Thr Gly  Ala Ser Val 
        995                 1000                 1005             


Leu Gly  Pro Arg Arg Leu Asp  Lys Leu Gly Leu Gly  Ala Asp Val 
    1010                 1015                 1020             


Ala Gln  Val Thr Gly Ala Leu  Arg Ser Gly Arg Gly  Lys Met Leu 
    1025                 1030                 1035             


Leu Phe  Ser Gly Arg Arg Leu  Trp Arg Phe Asp Val  Lys Ala Gln 
    1040                 1045                 1050             


Met Val  Asp Pro Arg Ser Ala  Ser Glu Val Asp Arg  Met Phe Pro 
    1055                 1060                 1065             


Gly Val  Pro Leu Asp Thr His  Asp Val Phe Gln Tyr  Arg Glu Lys 
    1070                 1075                 1080             


Ala Tyr  Phe Cys Gln Asp Arg  Phe Tyr Trp Arg Val  Ser Ser Arg 
    1085                 1090                 1095             


Ser Glu  Leu Asn Gln Val Asp  Gln Val Gly Tyr Val  Thr Tyr Asp 
    1100                 1105                 1110             


Ile Leu  Gln Cys Pro Glu Asp  
    1115                 1120 


<210>  66
<211>  1125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-MMP9-LL bi-specific T-cell engager 
       construct

<400>  66

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro Asp Lys 
            20                  25                  30          


Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile 
        35                  40                  45              


Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly 
    50                  55                  60                  


Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe Pro Arg 
65                  70                  75                  80  


Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp Phe Ser 
                85                  90                  95      


Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys 
            100                 105                 110         


Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly 
        115                 120                 125             


Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg 
145                 150                 155                 160 


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
            180                 185                 190         


Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn 
        195                 200                 205             


Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser 
    210                 215                 220                 


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp 
                245                 250                 255     


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly 
            260                 265                 270         


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu 
        275                 280                 285             


Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr 
    290                 295                 300                 


Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln 
305                 310                 315                 320 


Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys 
                325                 330                 335     


Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            340                 345                 350         


Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr 
        355                 360                 365             


Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly 
    370                 375                 380                 


Thr Lys Leu Glu Leu Lys His His His His His His Arg Arg Lys Arg 
385                 390                 395                 400 


Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
                405                 410                 415     


Gly Pro Met Ser Leu Trp Gln Pro Leu Val Leu Val Leu Leu Val Leu 
            420                 425                 430         


Gly Cys Cys Phe Ala Ala Pro Arg Gln Arg Gln Ser Thr Leu Val Leu 
        435                 440                 445             


Phe Pro Gly Asp Leu Arg Thr Asn Leu Thr Asp Arg Gln Leu Ala Glu 
    450                 455                 460                 


Glu Tyr Leu Tyr Arg Tyr Gly Tyr Thr Arg Val Ala Glu Met Arg Gly 
465                 470                 475                 480 


Glu Ser Lys Ser Leu Gly Pro Ala Leu Leu Leu Leu Gln Lys Gln Leu 
                485                 490                 495     


Ser Leu Pro Glu Thr Gly Glu Leu Asp Ser Ala Thr Leu Lys Ala Met 
            500                 505                 510         


Arg Thr Pro Arg Cys Gly Val Pro Asp Leu Gly Arg Phe Gln Thr Phe 
        515                 520                 525             


Glu Gly Asp Leu Lys Trp His His His Asn Ile Thr Tyr Trp Ile Gln 
    530                 535                 540                 


Asn Tyr Ser Glu Asp Leu Pro Arg Ala Val Ile Asp Asp Ala Phe Ala 
545                 550                 555                 560 


Arg Ala Phe Ala Leu Trp Ser Ala Val Thr Pro Leu Thr Phe Thr Arg 
                565                 570                 575     


Val Tyr Ser Arg Asp Ala Asp Ile Val Ile Gln Phe Gly Val Ala Glu 
            580                 585                 590         


His Gly Asp Gly Tyr Pro Phe Asp Gly Lys Asp Gly Leu Leu Ala His 
        595                 600                 605             


Ala Phe Pro Pro Gly Pro Gly Ile Gln Gly Asp Ala His Phe Asp Asp 
    610                 615                 620                 


Asp Glu Leu Trp Ser Leu Gly Lys Gly Val Val Val Pro Thr Arg Phe 
625                 630                 635                 640 


Gly Asn Ala Asp Gly Ala Ala Cys His Phe Pro Phe Ile Phe Glu Gly 
                645                 650                 655     


Arg Ser Tyr Ser Ala Cys Thr Thr Asp Gly Arg Ser Asp Gly Leu Pro 
            660                 665                 670         


Trp Cys Ser Thr Thr Ala Asn Tyr Asp Thr Asp Asp Arg Phe Gly Phe 
        675                 680                 685             


Cys Pro Ser Glu Arg Leu Tyr Thr Arg Asp Gly Asn Ala Asp Gly Lys 
    690                 695                 700                 


Pro Cys Gln Phe Pro Phe Ile Phe Gln Gly Gln Ser Tyr Ser Ala Cys 
705                 710                 715                 720 


Thr Thr Asp Gly Arg Ser Asp Gly Tyr Arg Trp Cys Ala Thr Thr Ala 
                725                 730                 735     


Asn Tyr Asp Arg Asp Lys Leu Phe Gly Phe Cys Pro Thr Arg Ala Asp 
            740                 745                 750         


Ser Thr Val Met Gly Gly Asn Ser Ala Gly Glu Leu Cys Val Phe Pro 
        755                 760                 765             


Phe Thr Phe Leu Gly Lys Glu Tyr Ser Thr Cys Thr Ser Glu Gly Arg 
    770                 775                 780                 


Gly Asp Gly Arg Leu Trp Cys Ala Thr Thr Ser Asn Phe Asp Ser Asp 
785                 790                 795                 800 


Lys Lys Trp Gly Phe Cys Pro Asp Gln Gly Tyr Ser Leu Phe Leu Val 
                805                 810                 815     


Ala Ala His Glu Phe Gly His Ala Leu Gly Leu Asp His Ser Ser Val 
            820                 825                 830         


Pro Glu Ala Leu Met Tyr Pro Met Tyr Arg Phe Thr Glu Gly Pro Pro 
        835                 840                 845             


Leu His Lys Asp Asp Val Asn Gly Ile Arg His Leu Tyr Gly Pro Arg 
    850                 855                 860                 


Pro Glu Pro Glu Pro Arg Pro Pro Thr Thr Thr Thr Pro Gln Pro Thr 
865                 870                 875                 880 


Ala Pro Pro Thr Val Cys Pro Thr Gly Pro Pro Thr Val His Pro Ser 
                885                 890                 895     


Glu Arg Pro Thr Ala Gly Pro Thr Gly Pro Pro Ser Ala Gly Pro Thr 
            900                 905                 910         


Gly Pro Pro Thr Ala Gly Pro Ser Thr Ala Thr Thr Val Pro Leu Ser 
        915                 920                 925             


Pro Val Asp Asp Ala Cys Asn Val Asn Ile Phe Asp Ala Ile Ala Glu 
    930                 935                 940                 


Ile Gly Asn Gln Leu Tyr Leu Phe Lys Asp Gly Lys Tyr Trp Arg Phe 
945                 950                 955                 960 


Ser Glu Gly Arg Gly Ser Arg Pro Gln Gly Pro Phe Leu Ile Ala Asp 
                965                 970                 975     


Lys Trp Pro Ala Leu Pro Arg Lys Leu Asp Ser Val Phe Glu Glu Pro 
            980                 985                 990         


Leu Ser Lys Lys Leu Phe Phe Phe  Ser Gly Arg Gln Val  Trp Val Tyr 
        995                 1000                 1005             


Thr Gly  Ala Ser Val Leu Gly  Pro Arg Arg Leu Asp  Lys Leu Gly 
    1010                 1015                 1020             


Leu Gly  Ala Asp Val Ala Gln  Val Thr Gly Ala Leu  Arg Ser Gly 
    1025                 1030                 1035             


Arg Gly  Lys Met Leu Leu Phe  Ser Gly Arg Arg Leu  Trp Arg Phe 
    1040                 1045                 1050             


Asp Val  Lys Ala Gln Met Val  Asp Pro Arg Ser Ala  Ser Glu Val 
    1055                 1060                 1065             


Asp Arg  Met Phe Pro Gly Val  Pro Leu Asp Thr His  Asp Val Phe 
    1070                 1075                 1080             


Gln Tyr  Arg Glu Lys Ala Tyr  Phe Cys Gln Asp Arg  Phe Tyr Trp 
    1085                 1090                 1095             


Arg Val  Ser Ser Arg Ser Glu  Leu Asn Gln Val Asp  Gln Val Gly 
    1100                 1105                 1110             


Tyr Val  Thr Tyr Asp Ile Leu  Gln Cys Pro Glu Asp  
    1115                 1120                 1125 


<210>  67
<211>  2814
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding SIRP1alpha-PDL1-CD3-Fc-SL 
       bi-specific T-cell engager construct

<400>  67
atggaaaccg atacacttct gttgtgggtg ctgctgctgt gggtccctgg ttcaacaggc       60

gattatccct acgatgtgcc cgactacgca ggcgctcagc cagctgatga tatccagatg      120

acacagagcc catcatctct gtctgcaagc gtaggagacc gagtcaccat tacatgcaga      180

gcctcccaag acgtttccac agcagtggcc tggtatcagc aaaaacctgg taaggcgccc      240

aagcttctca tctattcagc cagttttctg tatagcggcg ttcccagccg attctctggc      300

tctggatccg gcacggactt tactttgaca atttcctctc ttcagcccga agattttgca      360

acctactact gtcagcaata tctctaccat ccagccacat tcggacaggg caccaaagtc      420

gaaatcaaaa gaggcggcgg cggcagtggc ggcgggggtt caggaggcgg gggttctgaa      480

gtgcaactcg ttgaaagcgt aggagggctt gtccaacctg gcgggtcact gcggttgagc      540

tgcgccgcaa gcggattcac cttctcagac tcttggatcc attgggtgcg ccaggctccc      600

ggaaaaggct tggaatgggt tgcttggatt tcaccgtatg gcggttccac atactacgct      660

gacagcgtta agggtcgatt caccatctct gcagatactt caaaaaacac agcctacctt      720

cagatgaata gtttgcgcgc cgaggacaca gcggtttatt attgtgccct aagacattgg      780

cccggcggtt tcgactactg ggggcaaggt acgttggtga ctgtgagcgc cgtagatgaa      840

gcaaaatctt gtgacaaaac ccatacctgc ccaccatgcc cagccccaga acttcttggc      900

gtaccctctg tcttcctttt ccctccgaag cccaaggata ccctgatgat cagccgaacc      960

ccggaggtaa catgtgtggt ggtcgatgtt agccatgagg atcctgaagt caaatttaac     1020

tggtatgtag acggtgttga ggtgcacaac gctaaaacta agcccaggga ggagcagtac     1080

aactcaacct atcgcgtcgt atctgtgctt accgtcctgc atcaagactg gctcaatggt     1140

aaggaatata aatgtaaagt gagtaacaag gcactgccag cacctatcga aaaaaccatc     1200

tcaaaggcga agggacagcc cagggaaccc caggtctata ctctgcaacc ttctcgggat     1260

gaattgacca agaaccaagt tagcctgaca tgtctggtga aaggtttcta tccaagcgat     1320

atagctgtcg agtgggagtc caatggccaa cctgagaaca attataagac caccccaccc     1380

gttctggaca gcgacggatc ctttttcctg tactcaaaac tcactgtcga taaatcaaga     1440

tggcaacaag gcaacgtttt tagctgtagc gtgatgcacg aagcacttca taatcactat     1500

acacagaagt cactctctct ttctccagga aaggttgacg aacagaaatt gatatccgag     1560

gaagatctca ataggaggaa gagagaaggc agggggagcc ttctcacttg cggcgatgtc     1620

gaggaaaatc cggggcctat ggagaccgat accctgctct tgtgggtttt gcttctttgg     1680

gtgccaggat ctacaggtga tgaagaagaa ttgcagatca tccaaccaga caaatccgta     1740

ctcgtggccg caggagagac cgctaccctc agatgtacca tcacttctct cttccccgtt     1800

ggccccatcc agtggtttcg aggcgcagga ccaggacgag tgcttattta caatcaacga     1860

cagggcccat tcccaagagt gacaacagta tccgatacca ccaagcgcaa taatatggac     1920

tttagcatta gaatcggcaa cataacaccc gctgacgccg gtacatacta ttgtattaaa     1980

tttcgaaagg gctcaccaga cgacgtggaa tttaagtcag gggccggaac cgaactctca     2040

gttagagcaa aaccttctgc tagcgacatc aagctgcagc agagcggcgc cgagctggcc     2100

aggcccggcg ccagcgtgaa gatgagctgc aagaccagcg gctacacctt caccaggtac     2160

accatgcact gggtgaagca gaggcccggc cagggcctgg agtggatcgg ctacatcaac     2220

cccagcaggg gctacaccaa ctacaaccag aagttcaagg acaaggccac cctgaccacc     2280

gacaagagca gcagcaccgc ctacatgcag ctgagcagcc tgaccagcga ggacagcgcc     2340

gtgtactact gcgccaggta ctacgacgac cactactgcc tggactactg gggccagggc     2400

accaccctga ccgtgagcag cgtggagggc ggcagcggcg gcagcggcgg cagcggcggc     2460

agcggcggcg tggacgacat ccagctgacc cagagccccg ccatcatgag cgccagcccc     2520

ggcgagaagg tgaccatgac ctgcagggcc agcagcagcg tgagctacat gaactggtac     2580

cagcagaaga gcggcaccag ccccaagagg tggatctacg acaccagcaa ggtggccagc     2640

ggcgtgccct acaggttcag cggcagcggc agcggcacca gctacagcct gaccatcagc     2700

agcatggagg ccgaggacgc cgccacctac tactgccagc agtggagcag caaccccctg     2760

acctccggcg ccggcaccaa gctggagctg aagcaccacc atcatcacca ctga           2814


<210>  68
<211>  937
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-PDL1-CD3-Fc-SL bi-specific T-cell engager 
       construct

<400>  68

Met Glu Thr Asp Arg Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ala 
            20                  25                  30          


Gln Pro Ala Asp Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
        35                  40                  45              


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
    50                  55                  60                  


Val Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
65                  70                  75                  80  


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
                85                  90                  95      


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
            100                 105                 110         


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu 
        115                 120                 125             


Tyr His Pro Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
145                 150                 155                 160 


Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
                165                 170                 175     


Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp 
            180                 185                 190         


Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala 
        195                 200                 205             


Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
    210                 215                 220                 


Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu 
225                 230                 235                 240 


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                245                 250                 255     


Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            260                 265                 270         


Val Thr Val Ser Ala Val Asp Glu Ala Lys Ser Cys Asp Lys Thr His 
        275                 280                 285             


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
    290                 295                 300                 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
305                 310                 315                 320 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
                325                 330                 335     


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
            340                 345                 350         


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
        355                 360                 365             


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
    370                 375                 380                 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
385                 390                 395                 400 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
                405                 410                 415     


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
            420                 425                 430         


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
        435                 440                 445             


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
    450                 455                 460                 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
465                 470                 475                 480 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
                485                 490                 495     


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Val 
            500                 505                 510         


Asp Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Arg Arg Lys Arg 
        515                 520                 525             


Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
    530                 535                 540                 


Gly Pro Met Glu Thr Asp Arg Leu Leu Leu Trp Val Leu Leu Leu Trp 
545                 550                 555                 560 


Val Pro Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro 
                565                 570                 575     


Asp Lys Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys 
            580                 585                 590         


Thr Ile Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly 
        595                 600                 605             


Ala Gly Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe 
    610                 615                 620                 


Pro Arg Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp 
625                 630                 635                 640 


Phe Ser Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr 
                645                 650                 655     


Tyr Cys Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys 
            660                 665                 670         


Ser Gly Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser 
        675                 680                 685             


Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
    690                 695                 700                 


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Arg Tyr 
705                 710                 715                 720 


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
                725                 730                 735     


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
            740                 745                 750         


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
        755                 760                 765             


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
    770                 775                 780                 


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
785                 790                 795                 800 


Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly 
                805                 810                 815     


Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile Gln Leu Thr Gln Ser 
            820                 825                 830         


Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys 
        835                 840                 845             


Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser 
    850                 855                 860                 


Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Val Ala Ser 
865                 870                 875                 880 


Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser 
                885                 890                 895     


Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys 
            900                 905                 910         


Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu 
        915                 920                 925             


Glu Leu Lys His His His His His His 
    930                 935         


<210>  69
<211>  2829
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized polynucleotide encoding SIRP1alpha-PDL1-CD3-Fc-LL 
       bi-specific T-cell engager construct

<400>  69
atggaaaccg atacacttct gttgtgggtg ctgctgctgt gggtccctgg ttcaacaggc       60

gattatccct acgatgtgcc cgactacgca ggcgctcagc cagctgatga tatccagatg      120

acacagagcc catcatctct gtctgcaagc gtaggagacc gagtcaccat tacatgcaga      180

gcctcccaag acgtttccac agcagtggcc tggtatcagc aaaaacctgg taaggcgccc      240

aagcttctca tctattcagc cagttttctg tatagcggcg ttcccagccg attctctggc      300

tctggatccg gcacggactt tactttgaca atttcctctc ttcagcccga agattttgca      360

acctactact gtcagcaata tctctaccat ccagccacat tcggacaggg caccaaagtc      420

gaaatcaaaa gaggcggcgg cggcagtggc ggcgggggtt caggaggcgg gggttctgaa      480

gtgcaactcg ttgaaagcgt aggagggctt gtccaacctg gcgggtcact gcggttgagc      540

tgcgccgcaa gcggattcac cttctcagac tcttggatcc attgggtgcg ccaggctccc      600

ggaaaaggct tggaatgggt tgcttggatt tcaccgtatg gcggttccac atactacgct      660

gacagcgtta agggtcgatt caccatctct gcagatactt caaaaaacac agcctacctt      720

cagatgaata gtttgcgcgc cgaggacaca gcggtttatt attgtgccct aagacattgg      780

cccggcggtt tcgactactg ggggcaaggt acgttggtga ctgtgagcgc cgtagatgaa      840

gcaaaatctt gtgacaaaac ccatacctgc ccaccatgcc cagccccaga acttcttggc      900

gtaccctctg tcttcctttt ccctccgaag cccaaggata ccctgatgat cagccgaacc      960

ccggaggtaa catgtgtggt ggtcgatgtt agccatgagg atcctgaagt caaatttaac     1020

tggtatgtag acggtgttga ggtgcacaac gctaaaacta agcccaggga ggagcagtac     1080

aactcaacct atcgcgtcgt atctgtgctt accgtcctgc atcaagactg gctcaatggt     1140

aaggaatata aatgtaaagt gagtaacaag gcactgccag cacctatcga aaaaaccatc     1200

tcaaaggcga agggacagcc cagggaaccc caggtctata ctctgcaacc ttctcgggat     1260

gaattgacca agaaccaagt tagcctgaca tgtctggtga aaggtttcta tccaagcgat     1320

atagctgtcg agtgggagtc caatggccaa cctgagaaca attataagac caccccaccc     1380

gttctggaca gcgacggatc ctttttcctg tactcaaaac tcactgtcga taaatcaaga     1440

tggcaacaag gcaacgtttt tagctgtagc gtgatgcacg aagcacttca taatcactat     1500

acacagaagt cactctctct ttctccagga aaggttgacg aacagaaatt gatatccgag     1560

gaagatctca ataggaggaa gagagaaggc agggggagcc ttctcacttg cggcgatgtc     1620

gaggaaaatc cggggcctat ggagaccgat accctgctct tgtgggtttt gcttctttgg     1680

gtgccaggat ctacaggtga tgaagaagaa ttgcagatca tccaaccaga caaatccgta     1740

ctcgtggccg caggagagac cgctaccctc agatgtacca tcacttctct cttccccgtt     1800

ggccccatcc agtggtttcg aggcgcagga ccaggacgag tgcttattta caatcaacga     1860

cagggcccat tcccaagagt gacaacagta tccgatacca ccaagcgcaa taatatggac     1920

tttagcatta gaatcggcaa cataacaccc gctgacgccg gtacatacta ttgtattaaa     1980

tttcgaaagg gctcaccaga cgacgtggaa tttaagtcag gggccggaac cgaactctca     2040

gttagagcaa aaccttctgc tagcggcggc ggcggcagcg acatcaagct gcagcagagc     2100

ggcgccgagc tggccaggcc cggcgccagc gtgaagatga gctgcaagac cagcggctac     2160

accttcacca ggtacaccat gcactgggtg aagcagaggc ccggccaggg cctggagtgg     2220

atcggctaca tcaaccccag caggggctac accaactaca accagaagtt caaggacaag     2280

gccaccctga ccaccgacaa gagcagcagc accgcctaca tgcagctgag cagcctgacc     2340

agcgaggaca gcgccgtgta ctactgcgcc aggtactacg acgaccacta ctgcctggac     2400

tactggggcc agggcaccac cctgaccgtg agcagcgtgg agggcggcag cggcggcagc     2460

ggcggcagcg gcggcagcgg cggcgtggac gacatccagc tgacccagag ccccgccatc     2520

atgagcgcca gccccggcga gaaggtgacc atgacctgca gggccagcag cagcgtgagc     2580

tacatgaact ggtaccagca gaagagcggc accagcccca agaggtggat ctacgacacc     2640

agcaaggtgg ccagcggcgt gccctacagg ttcagcggca gcggcagcgg caccagctac     2700

agcctgacca tcagcagcat ggaggccgag gacgccgcca cctactactg ccagcagtgg     2760

agcagcaacc ccctgacctt cggcgccggc accaagctgg agctgaagca ccaccaccac     2820

caccactag                                                             2829


<210>  70
<211>  942
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized SIRP1alpha-PDL1-CD3-Fc-LL bi-specific T-cell engager 
       construct

<400>  70

Met Glu Thr Asp Arg Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Ala 
            20                  25                  30          


Gln Pro Ala Asp Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
        35                  40                  45              


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
    50                  55                  60                  


Val Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
65                  70                  75                  80  


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
                85                  90                  95      


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
            100                 105                 110         


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu 
        115                 120                 125             


Tyr His Pro Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
145                 150                 155                 160 


Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
                165                 170                 175     


Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp 
            180                 185                 190         


Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala 
        195                 200                 205             


Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
    210                 215                 220                 


Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu 
225                 230                 235                 240 


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                245                 250                 255     


Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            260                 265                 270         


Val Thr Val Ser Ala Val Asp Glu Ala Lys Ser Cys Asp Lys Thr His 
        275                 280                 285             


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
    290                 295                 300                 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
305                 310                 315                 320 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
                325                 330                 335     


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
            340                 345                 350         


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
        355                 360                 365             


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
    370                 375                 380                 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
385                 390                 395                 400 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
                405                 410                 415     


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
            420                 425                 430         


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
        435                 440                 445             


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
    450                 455                 460                 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
465                 470                 475                 480 


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
                485                 490                 495     


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Val 
            500                 505                 510         


Asp Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asn Arg Arg Lys Arg 
        515                 520                 525             


Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
    530                 535                 540                 


Gly Pro Met Glu Thr Asp Arg Leu Leu Leu Trp Val Leu Leu Leu Trp 
545                 550                 555                 560 


Val Pro Gly Ser Thr Gly Asp Glu Glu Glu Leu Gln Ile Ile Gln Pro 
                565                 570                 575     


Asp Lys Ser Val Leu Val Ala Ala Gly Glu Thr Ala Thr Leu Arg Cys 
            580                 585                 590         


Thr Ile Thr Ser Leu Phe Pro Val Gly Pro Ile Gln Trp Phe Arg Gly 
        595                 600                 605             


Ala Gly Pro Gly Arg Val Leu Ile Tyr Asn Gln Arg Gln Gly Pro Phe 
    610                 615                 620                 


Pro Arg Val Thr Thr Val Ser Asp Thr Thr Lys Arg Asn Asn Met Asp 
625                 630                 635                 640 


Phe Ser Ile Arg Ile Gly Asn Ile Thr Pro Ala Asp Ala Gly Thr Tyr 
                645                 650                 655     


Tyr Cys Ile Lys Phe Arg Lys Gly Ser Pro Asp Asp Val Glu Phe Lys 
            660                 665                 670         


Ser Gly Ala Gly Thr Glu Leu Ser Val Arg Ala Lys Pro Ser Ala Ser 
        675                 680                 685             


Gly Gly Gly Gly Ser Asp Ile Lys Leu Gln Gln Ser Gly Ala Glu Leu 
    690                 695                 700                 


Ala Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr 
705                 710                 715                 720 


Thr Phe Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln 
                725                 730                 735     


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn 
            740                 745                 750         


Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser 
        755                 760                 765             


Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
    770                 775                 780                 


Ala Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp 
785                 790                 795                 800 


Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly 
                805                 810                 815     


Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Asp Ile 
            820                 825                 830         


Gln Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys 
        835                 840                 845             


Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Tyr Met Asn Trp 
    850                 855                 860                 


Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr 
865                 870                 875                 880 


Ser Lys Val Ala Ser Gly Val Pro Tyr Arg Phe Ser Gly Ser Gly Ser 
                885                 890                 895     


Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala 
            900                 905                 910         


Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr Phe Gly 
        915                 920                 925             


Ala Gly Thr Lys Leu Glu Leu Lys His His His His His His 
    930                 935                 940         


<210>  71
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-FAP light chain sequence

<400>  71

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Gly Val Asn Phe Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Phe 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe Asn Pro Pro Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105         


<210>  72
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-FAP heavy chain sequence

<400>  72

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Asn Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Asn 
            20                  25                  30          


Gly Ile Asn Trp Leu Lys Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Tyr Pro Arg Ser Thr Asn Thr Leu Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Arg Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Thr Leu Thr Ala Pro Phe Ala Phe Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ala 
        115         


<210>  73
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-FAP scFv sequence

<400>  73

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Asn Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Asn 
            20                  25                  30          


Gly Ile Asn Trp Leu Lys Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Tyr Pro Arg Ser Thr Asn Thr Leu Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Arg Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Thr Leu Thr Ala Pro Phe Ala Phe Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ala Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser 
        115                 120                 125             


Gly Glu Gly Ser Thr Lys Gly Gln Ile Val Leu Thr Gln Ser Pro Ala 
    130                 135                 140                 


Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala 
145                 150                 155                 160 


Ser Ser Gly Val Asn Phe Met His Trp Tyr Gln Gln Lys Ser Gly Thr 
                165                 170                 175     


Ser Pro Lys Arg Trp Ile Phe Asp Thr Ser Lys Leu Ala Ser Gly Val 
            180                 185                 190         


Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr 
        195                 200                 205             


Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 


Trp Ser Phe Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
225                 230                 235                 240 


Lys Arg 
        


<210>  74
<211>  1545
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide sequence encoding anti-FAP - anti-CD3 
       BiTE sequence

<400>  74
atggttttgc ttgtgaccag cctcctgctc tgtgaactgc ctcatcctgc attcctgttg       60

atcccacagg tgcagctcca gcagagtggc gcagagctcg ctcgcccagg cgcttctgtg      120

aatctgagtt gtaaggcctc cggatatact tttacgaaca acggcatcaa ctggctgaag      180

cagcggaccg gccagggcct ggagtggatc ggcgaaatat acccccggtc cacaaacact      240

ctctataacg agaagtttaa gggcaaagca actctgaccg cggacaggtc ctctaacaca      300

gcctatatgg agctgagaag cttgacgagt gaggactccg ctgtctattt ttgcgcccga      360

actctgaccg ctccttttgc tttttggggc cagggcacgc tcgtgaccgt aagtgcgggc      420

tccactagcg gttccggcaa acctggcagc ggagaaggca gcaccaaagg gcagatcgtc      480

ctgacgcagt ctccagccat catgagcgcc tcacccggcg aaaaggtgac catgacctgc      540

tcagcctctt ctggtgtgaa tttcatgcac tggtaccagc aaaaaagtgg gacctcccct      600

aaaaggtgga tcttcgatac cagcaaactg gcttctggcg ttcccgcaag gtttagcggc      660

tctggttccg gcacatcata cagcctgacg atcagcagca tggaggcaga agacgcagct      720

acctattact gccagcaatg gagctttaac ccacctactt tcggaggagg aacaaagctg      780

gaaataaaaa gaggtggtgg tggatcagag gtgcagctgg tggagtctgg gggaggcttg      840

gtgcagcctg gaaagtccct gaaactctcc tgtgaggcct ctggattcac cttcagcggc      900

tatggcatgc actgggtccg ccaggctcca gggagggggc tggagtcggt cgcatacatt      960

actagtagta gtattaatat caaatatgct gacgctgtga aaggccggtt caccgtctcc     1020

agagacaatg ccaagaactt actgtttcta caaatgaaca ttctcaagtc tgaggacaca     1080

gccatgtact actgtgcaag attcgactgg gacaaaaatt actggggcca aggaaccatg     1140

gtcaccgtct cctcaggtgg tggtggatca ggtggaggcg gaagtggagg tggcggatcc     1200

gacatccaga tgacccagtc tccatcatca ctgcctgcct ccctgggaga cagagtcact     1260

atcaattgtc aggccagtca ggacattagc aattatttaa actggtacca gcagaaacca     1320

gggaaagctc ctaagctcct gatctattat acaaataaat tggcagatgg agtcccatca     1380

aggttcagtg gcagtggttc tgggagagat tcttctttca ctatcagcag cctggaatcc     1440

gaagatattg gatcttatta ctgtcaacag tattataact atccgtggac gttcggacct     1500

ggcaccaagc tggaaatcaa acggcaccac catcatcacc actga                     1545


<210>  75
<211>  512
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-FAP - anti-Cd3 BiTE sequence

<400>  75

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala 
            20                  25                  30          


Arg Pro Gly Ala Ser Val Asn Leu Ser Cys Lys Ala Ser Gly Tyr Thr 
        35                  40                  45              


Phe Thr Asn Asn Gly Ile Asn Trp Leu Lys Gln Arg Thr Gly Gln Gly 
    50                  55                  60                  


Leu Glu Trp Ile Gly Glu Ile Tyr Pro Arg Ser Thr Asn Thr Leu Tyr 
65                  70                  75                  80  


Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Arg Ser Ser 
                85                  90                  95      


Asn Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala 
            100                 105                 110         


Val Tyr Phe Cys Ala Arg Thr Leu Thr Ala Pro Phe Ala Phe Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ala Gly Ser Thr Ser Gly Ser Gly 
    130                 135                 140                 


Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Ile Val Leu Thr 
145                 150                 155                 160 


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
                165                 170                 175     


Thr Cys Ser Ala Ser Ser Gly Val Asn Phe Met His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Phe Asp Thr Ser Lys Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser 
    210                 215                 220                 


Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Trp Ser Phe Asn Pro Pro Thr Phe Gly Gly Gly Thr 
                245                 250                 255     


Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
            260                 265                 270         


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Lys Ser Leu Lys Leu Ser 
        275                 280                 285             


Cys Glu Ala Ser Gly Phe Thr Phe Ser Gly Tyr Gly Met His Trp Val 
    290                 295                 300                 


Arg Gln Ala Pro Gly Arg Gly Leu Glu Ser Val Ala Tyr Ile Thr Ser 
305                 310                 315                 320 


Ser Ser Ile Asn Ile Lys Tyr Ala Asp Ala Val Lys Gly Arg Phe Thr 
                325                 330                 335     


Val Ser Arg Asp Asn Ala Lys Asn Leu Leu Phe Leu Gln Met Asn Ile 
            340                 345                 350         


Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Arg Phe Asp Trp 
        355                 360                 365             


Asp Lys Asn Tyr Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly 
    370                 375                 380                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 
385                 390                 395                 400 


Gln Met Thr Gln Ser Pro Ser Ser Leu Pro Ala Ser Leu Gly Asp Arg 
                405                 410                 415     


Val Thr Ile Asn Cys Gln Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn 
            420                 425                 430         


Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Tyr 
        435                 440                 445             


Thr Asn Lys Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
    450                 455                 460                 


Ser Gly Arg Asp Ser Ser Phe Thr Ile Ser Ser Leu Glu Ser Glu Asp 
465                 470                 475                 480 


Ile Gly Ser Tyr Tyr Cys Gln Gln Tyr Tyr Asn Tyr Pro Trp Thr Phe 
                485                 490                 495     


Gly Pro Gly Thr Lys Leu Glu Ile Lys Arg His His His His His His 
            500                 505                 510         


<210>  76
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-mouse CD3 heavy chain sequence

<400>  76

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Lys 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Glu Ala Ser Gly Phe Thr Phe Ser Gly Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Arg Gly Leu Glu Ser Val 
        35                  40                  45              


Ala Tyr Ile Thr Ser Ser Ser Ile Asn Ile Lys Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Asn Leu Leu Phe 
65                  70                  75                  80  


Leu Gln Met Asn Ile Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Phe Asp Trp Asp Lys Asn Tyr Trp Gly Gln Gly Thr Met Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  77
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-mouse CD3 light chain

<400>  77

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Pro Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Asn Lys Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Arg Asp Ser Ser Phe Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Ile Gly Ser Tyr Tyr Cys Gln Gln Tyr Tyr Asn Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  78
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-mouse CD3 scFv

<400>  78

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Lys 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Glu Ala Ser Gly Phe Thr Phe Ser Gly Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Arg Gly Leu Glu Ser Val 
        35                  40                  45              


Ala Tyr Ile Thr Ser Ser Ser Ile Asn Ile Lys Tyr Ala Asp Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Asn Leu Leu Phe 
65                  70                  75                  80  


Leu Gln Met Asn Ile Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Phe Asp Trp Asp Lys Asn Tyr Trp Gly Gln Gly Thr Met Val 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Pro Ala 
    130                 135                 140                 


Ser Leu Gly Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Asp Ile 
145                 150                 155                 160 


Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
                165                 170                 175     


Leu Leu Ile Tyr Tyr Thr Asn Lys Leu Ala Asp Gly Val Pro Ser Arg 
            180                 185                 190         


Phe Ser Gly Ser Gly Ser Gly Arg Asp Ser Ser Phe Thr Ile Ser Ser 
        195                 200                 205             


Leu Glu Ser Glu Asp Ile Gly Ser Tyr Tyr Cys Gln Gln Tyr Tyr Asn 
    210                 215                 220                 


Tyr Pro Trp Thr Phe Gly Pro Gly Thr Lys Leu Glu Ile Lys Arg 
225                 230                 235                 


<210>  79
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker sequence

<400>  79

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  80
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CCL4 sequence

<400>  80

Met Lys Leu Cys Val Thr Val Leu Ser Leu Leu Met Leu Val Ala Ala 
1               5                   10                  15      


Phe Cys Ser Pro Ala Leu Ser Ala Pro Met Gly Ser Asp Pro Pro Thr 
            20                  25                  30          


Ala Cys Cys Phe Ser Tyr Thr Ala Arg Lys Leu Pro Arg Asn Phe Val 
        35                  40                  45              


Val Asp Tyr Tyr Glu Thr Ser Ser Leu Cys Ser Gln Pro Ala Val Val 
    50                  55                  60                  


Phe Gln Thr Lys Arg Ser Lys Gln Val Cys Ala Asp Pro Ser Glu Ser 
65                  70                  75                  80  


Trp Val Gln Glu Tyr Val Tyr Asp Leu Glu Leu Asn 
                85                  90          


