                         SEQUENCE LISTING

<110>  FATE THERAPEUTICS, INC.
 
<120>  GENERATION OF INDUCED PLURIPOTENT CELLS BY CRISPR ACTIVATION

<130>  13601-222-228

<140>  TBA
<141>  2018-12-27

<150>  US 62/611,202
<151>  2017-12-28

<160>  175   

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-321 for targeting Oct4 promoter

<400>  1
cagcccactc agccatcctc                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-248 for targeting Oct4 promoter

<400>  2
atccgagcaa ctggtttgtg                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-226 for targeting Oct4 promoter

<400>  3
gtgtccggtg acccaaggca                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-157 for targeting Oct4 promoter

<400>  4
ggacaggaca acccttagga                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-127 for targeting Oct4 promoter

<400>  5
aacctccgtc tggaagacac                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-71 for targeting Oct4 promoter

<400>  6
gggtggagga gcagagctgt                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-2135 for targeting Oct4 enhancer

<400>  7
agacaggact gctgggctgc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-2066 for targeting Oct4 enhancer

<400>  8
gccctgggag gaactgggtg                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-2014 for targeting Oct4 enhancer

<400>  9
cccagggagg ttgagagttc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-1965 for targeting Oct4 enhancer

<400>  10
gcatgatagc tctgccctgg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA O-1914 for targeting Oct4 enhancer

<400>  11
taaggaaggg ctaggacgag                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-430 for targeting Sox2 promoter

<400>  12
caactagtat ttcaggaagc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-398 for targeting Sox2 promoter

<400>  13
gcaaggccca tgggtggttc                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-348 for targeting Sox2 promoter

<400>  14
cttggataca taagggtgga                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-326 for targeting Sox2 promoter

<400>  15
agagccaata ttccgtagca                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-276 for targeting Sox2 promoter

<400>  16
tttacccact tccttcgaac                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-262 for targeting Sox2 promoter

<400>  17
cacggcgcac gcctgttcga                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-192 for targeting Sox2 promoter

<400>  18
gcgggcccgc agccggccgc                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-148 for targeting Sox2 promoter

<400>  19
gcgctctgct gggctcggct                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-136 for targeting Sox2 promoter

<400>  20
gctcggctcg gcggcgcggc                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA S-84 for targeting Sox2 promoter

<400>  21
gcgaggctgg gctcgggcgc                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-475 for targeting Klf4 promoter

<400>  22
ggacaagcgc gtacgcgagc                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-380 for targeting Klf4 promoter

<400>  23
tgggctcgaa agtcctgcca                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-309 for targeting Klf4 promoter

<400>  24
ccacgccgta ctcccagcgc                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-293 for targeting Klf4 promoter

<400>  25
ggcgacggcg gctccggcgc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-271 for targeting Klf4 promoter

<400>  26
caccgccgcc ggcgtcagca                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-249 for targeting Klf4 promoter

<400>  27
gctccagccc gccagctgcc                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-232 for targeting Klf4 promoter

<400>  28
gcctggctgg cgtcacggcc                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-171 for targeting Klf4 promoter

<400>  29
taaacaaact ccgcgcacgt                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-147 for targeting Klf4 promoter

<400>  30
gctaccatgg caacgcgcag                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA K-67 for targeting Klf4 promoter

<400>  31
cgcgcgccgc cacagggagg                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-444 for targeting cMyc promoter

<400>  32
gacgaacgaa tgagttatct                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-395 for targeting cMyc promoter

<400>  33
ccaggcgtct ctctaaggct                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-347 for targeting cMyc promoter

<400>  34
acacaatacg ccatgtaccc                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-305 for targeting cMyc promoter

<400>  35
tgcggtgact gatatacgca                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-249 for targeting cMyc promoter

<400>  36
acaaccgtac agaaagggaa                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-242 for targeting cMyc promoter

<400>  37
tagtcctttc cctttctgta                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-175 for targeting cMyc promoter

<400>  38
cgctattact gtttacaccc                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-154 for targeting cMyc promoter

<400>  39
cagcccagta ctccggctcc                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-124 for targeting cMyc promoter

<400>  40
ggctcctcct cctctttccc                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA M-82 for targeting cMyc promoter

<400>  41
cgagttccca aagcagaggg                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA Nr-404 for targeting Nr5a2 promoter

<400>  42
ccgccctctc acggaagcgg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA Nr-157 for targeting Nr5a2 promoter

<400>  43
gggcgtggag cccaggaagg                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA Nr-61 for targeting Nr5a2 promoter

<400>  44
gatggaatgt tcaagtggga                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA Nr-60 for targeting Nr5a2 promoter

<400>  45
tcccacttga acattccatc                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA G-419 for targeting Glis1 promoter

<400>  46
gggaggagca gaatcccgcc                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA G-215 for targeting Glis1 promoter

<400>  47
gggctgccgg accaagccaa                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA G-179 for targeting Glis1 promoter

<400>  48
gagcggctgt gggcagcagc                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA G-118 for targeting Glis1 promoter

<400>  49
ggccgtggcg gtggcggcgg                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA G-47 for targeting Glis1 promoter

<400>  50
gccgcgggcg cagcggctcg                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA C-345 for targeting Cebpa

<400>  51
gctcccgggc tccctagtgt                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA C-282 for targeting Cebpa

<400>  52
cacacacgtg gtccgtggtt                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA C-177 for targeting Cebpa

<400>  53
gtgctagtgg agagagatcg                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA C-135 for targeting Cebpa

<400>  54
ggaaagtcac aggagaaggc                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA C-100 for targeting Cebpa

<400>  55
gccagtagga tggtgcctgc                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA C-70 for targeting Cebpa

<400>  56
cgagacccgt ttggacacca                                                   20


<210>  57
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human gRNA sequence for targeting OCT4 promoter

<400>  57
ggggagaaac tgaggcga                                                     18


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human gRNA sequence for targeting OCT4 promoter

<400>  58
tctgtggggg acctgcactg                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human gRNA sequence for targeting Sox2 promoter

<400>  59
gtggctggca ggctggctct                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human gRNA sequence for targeting Klf4 promoter

<400>  60
gctgccatag caacgatgga                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human gRNA sequence for targeting c-Myc promoter 

<400>  61
ggttcccaaa gcagagggcg                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human gRNA sequence for targeting Lin28A promoter

<400>  62
gtgtcagaga ccggagttgt                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human gRNA sequence for targeting NANOG promoter 

<400>  63
gattaactga gaattcacaa                                                   20


<210>  64
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human gRNA sequence for targeting EEA-motif

<400>  64
cccagcactt tggg                                                         14


<210>  65
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Oct4 (total)

<400>  65
atcgccaatc agcttgg                                                      17


<210>  66
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Oct4 (total)

<400>  66
agaaccatac tcgaaccaca tcc                                               23


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Oct4 (endogenous)

<400>  67
taggtgagcc gtctttccac                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Oct4 (endogenous)

<400>  68
gcttagccag gttcgaggat                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Sox2 (total)

<400>  69
acagatgcaa ccgatgcacc                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Sox2 (total)

<400>  70
tggagttgta ctgcagggcg                                                   20


<210>  71
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Sox2 (endogenous)

<400>  71
gagaagtttg gagcccgag                                                    19


<210>  72
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Sox2 (endogenous)

<400>  72
gatctggcgg agaatagttg g                                                 21


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Klf4

<400>  73
gcacacctgc gaactcacac                                                   20


<210>  74
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Klf4

<400>  74
ccgtcccagt cacagtggta a                                                 21


<210>  75
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene c-Myc

<400>  75
ccaccagcag cgactctga                                                    19


<210>  76
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene c-Myc

<400>  76
tgcctcttct ccacagacac c                                                 21


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Nr5a2

<400>  77
atgggaagga agggacaatc                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Nr5a2

<400>  78
atacaaactc ccgctgatcg                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Glis1

<400>  79
ctccaagcat ccacactgtt                                                   20


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Glis1

<400>  80
gacaggatgc ctgaagcaag                                                   20


<210>  81
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Cebpa

<400>  81
caagaacagc aacgagtacc g                                                 21


<210>  82
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Cebpa

<400>  82
gtcactggtc aactccagca c                                                 21


<210>  83
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Nanog

<400>  83
cctccagcag atgcaagaac tc                                                22


<210>  84
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Nanog

<400>  84
cttcaaccac tggtttttct gcc                                               23


<210>  85
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Dppa2

<400>  85
tcaacgagaa ccaatctgag ga                                                22


<210>  86
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Dppa2

<400>  86
gcgtagcgta gtctgtgttt g                                                 21


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Esrrb

<400>  87
ctcgccaact cagattcgat                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Esrrb

<400>  88
agaagtgttg cacggctttg                                                   20


<210>  89
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Fgf4

<400>  89
cgtggtgagc atcttcggag tgg                                               23


<210>  90
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Fgf4

<400>  90
ccttcttggt ccgcccgttc tta                                               23


<210>  91
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Lin28

<400>  91
tgttctgtat tgggagtgag c                                                 21


<210>  92
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Lin28

<400>  92
gcttgcattc cttggcatg                                                    19


<210>  93
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Tet1

<400>  93
tctcactcat gttgcgggac cc                                                22


<210>  94
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Tet1

<400>  94
cgtcggagtt gaaatgggcg aa                                                22


<210>  95
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Utf1

<400>  95
tgtcccggtg actacgtct                                                    19


<210>  96
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Utf1

<400>  96
cccagaagta gctccgtctc t                                                 21


<210>  97
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Actin

<400>  97
atggagggga atacagccc                                                    19


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Actin

<400>  98
ttctttgcag ctccttcgtt                                                   20


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Oct4 (ChIP-2.7k)

<400>  99
tggcctggaa ctcagaaatc                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Oct4 (ChIP-2.7k)

<400>  100
tctgccccct ttaagagtca                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Oct4 (ChIP-1.4k)

<400>  101
cccaggctca gaactctgtc                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Oct4 (ChIP-1.4k)

<400>  102
tgctcctaca ccatgctctg                                                   20


<210>  103
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Oct4 (ChIP-0.2k)

<400>  103
ttgaaaatga aggcctcctg                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Oct4 (ChIP-0.2k)

<400>  104
agcgctatct gcctgtgtct                                                   20


<210>  105
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Sox2 (ChIP-0.4k)

<400>  105
cttgggtcta acttctcgtc tg                                                22


<210>  106
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Sox2 (ChIP-0.4k)

<400>  106
gtgtgccatt gtttctgcg                                                    19


<210>  107
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Nanog (ChIP-0.5k)

<400>  107
ccaacttact aaggtagccc g                                                 21


<210>  108
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Nanog (ChIP-0.5k)

<400>  108
ctttcagcac tcagcgtttc                                                   20


<210>  109
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Rex1 (ChIP-0.5k)

<400>  109
ccccgctaca aagtacacta g                                                 21


<210>  110
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Rex1 (ChIP-0.5k)

<400>  110
ctagaccgtt tgtagtcagt gg                                                22


<210>  111
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Ctnnbl1

<400>  111
agaacgacag tgagaaggtt g                                                 21


<210>  112
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Ctnnbl1

<400>  112
cattgtctat gatctcccca cg                                                22


<210>  113
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Tbc1d22b

<400>  113
ttctgtttat ctgggccatc c                                                 21


<210>  114
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Tbc1d22b

<400>  114
gtcaaagttc tccacgtcct c                                                 21


<210>  115
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Phf20

<400>  115
agtgtgaaga gtgccagtg                                                    19


<210>  116
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Phf20

<400>  116
ctcagccact ccttgtcata c                                                 21


<210>  117
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Rgr

<400>  117
gtacctatac gcagccatcg                                                   20


<210>  118
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Rgr

<400>  118
ttcctctaca gaccatctcc c                                                 21


<210>  119
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Zp3r

<400>  119
actgtcctga aatatacctg cc                                                22


<210>  120
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Zp3r

<400>  120
actttcccat tgaccaactc g                                                 21


<210>  121
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Lmbr1

<400>  121
gcggtgggta tgaaaggag                                                    19


<210>  122
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Lmbr1

<400>  122
aggaaacgat gtagagaatg gc                                                22


<210>  123
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene D130040H23Rik

<400>  123
gacctacagc aacctcactg                                                   20


<210>  124
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene D130040H23Rik

<400>  124
gcaaaggctt taccacactg                                                   20


<210>  125
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Gli3

<400>  125
ttcagcaagt ggttcctatg g                                                 21


<210>  126
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Gli3

<400>  126
ctgtcggctt aggatctgtt g                                                 21


<210>  127
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Vav3

<400>  127
tgtcaaaccc tctccatgtg                                                   20


<210>  128
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Vav3

<400>  128
tctttggtcc tgtgccttac                                                   20


<210>  129
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Renbp

<400>  129
cacagtgaag ccatgattgc                                                   20


<210>  130
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Renbp

<400>  130
agccaaacca ttccccatac                                                   20


<210>  131
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Rab11fip4os1

<400>  131
gtctctgatg gaaggatgct g                                                 21


<210>  132
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Rab11fip4os1

<400>  132
gccttaattt gttttgcctc gg                                                22


<210>  133
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Styk1

<400>  133
gaaaatcatg aagagaccca gc                                                22


<210>  134
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Styk1

<400>  134
catcggcaga tctagaagca g                                                 21


<210>  135
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Cyb5a

<400>  135
cagaagcaca aagacagcaa g                                                 21


<210>  136
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Cyb5a

<400>  136
aaattctcgg tagcatcacc c                                                 21


<210>  137
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Zak

<400>  137
gtaatggaga agtggatcgt gg                                                22


<210>  138
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Zak

<400>  138
ttcgttctgt cccactgtat g                                                 21


<210>  139
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Tec

<400>  139
gaaacagcaa catccccaaa g                                                 21


<210>  140
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Tec

<400>  140
cttccccttt gtactgaccg                                                   20


<210>  141
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Fbn2

<400>  141
acctgaatcc caacatctgc                                                   20


<210>  142
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Fbn2

<400>  142
ccaatctcac actcgtccac                                                   20


<210>  143
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Zfp653

<400>  143
ccacctctat agccagcatt g                                                 21


<210>  144
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Zfp653

<400>  144
ccatccactt ctgcctctg                                                    19


<210>  145
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Ptgis

<400>  145
aggatgaagg aaaagcacgg                                                   20


<210>  146
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Ptgis

<400>  146
catgaggaag atggcatagg g                                                 21


<210>  147
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Alg10b

<400>  147
caacttctac ttgctgtatt tgctc                                             25


<210>  148
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Alg10b

<400>  148
gacccagctt ctgtatagta aagg                                              24


<210>  149
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Sh3pxd2a

<400>  149
aggaaatcag tgtggttgtc c                                                 21


<210>  150
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Sh3pxd2a

<400>  150
agctccgagt tctcttgttt c                                                 21


<210>  151
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Tubb6

<400>  151
agaggcattt gaagacgagg                                                   20


<210>  152
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Tubb6

<400>  152
ccagtgacat gcttagacca g                                                 21


<210>  153
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Eva1c

<400>  153
accaaatgtg tagttcccag g                                                 21


<210>  154
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Eva1c

<400>  154
gaagactcgg ctattgacca g                                                 21


<210>  155
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Pou3f1

<400>  155
gctctgtgca gtgaccc                                                      17


<210>  156
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Pou3f1

<400>  156
gtaaaatcca aagcaaaacc gaataa                                            26


<210>  157
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Prickle2

<400>  157
aaccagagga aacgtgagaa c                                                 21


<210>  158
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Prickle2

<400>  158
gtgatgcaaa cacagcgatg                                                   20


<210>  159
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Map6

<400>  159
cagtgctacc aaacccgac                                                    19


<210>  160
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Map6

<400>  160
acagagcctt gatccttgtg                                                   20


<210>  161
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Tomm70a

<400>  161
cagtggcgga ttttgatgc                                                    19


<210>  162
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Tomm70a

<400>  162
aacctttcat agctgcctgg                                                   20


<210>  163
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of Gene Ube2g1

<400>  163
aatggaggga agacagaaac g                                                 21


<210>  164
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of Gene Ube2g1

<400>  164
cagtgccatg tttctcaatt gg                                                22


<210>  165
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for qPCR of sgRNA cassette (quantification)

<400>  165
ctatgtggac tacagactgg aaag                                              24


<210>  166
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for qPCR of sgRNA cassette (quantification)

<400>  166
ctagggaggt cgcagtatct                                                   20


<210>  167
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primers mU6-T2H-F to generate the dual-sgRNA constructs

<400>  167
ctaggatcca ttaggcgggt acagtgcagg ggaa                                   34


<210>  168
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primers mU6-T2H-R2 to generate the dual-sgRNA constructs

<400>  168
atacggttat ccacgcggcc gcctaatgga tcct                                   34


<210>  169
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer to clone an 83-bp SV40 nuclear localization
 site (NLS) with a linker 

<400>  169
tacaaaggtg gaggtcggac cgaaggcagc ggctccccca ag                          42


<210>  170
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer to clone an 83-bp SV40 nuclear localization
 site (NLS) with a linker  

<400>  170
aaatcgtcta aagcatccga ccctccgccg gaaccgccca                             40


<210>  171
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer to amplify p300core

<400>  171
agtgggcggt tccggcggag ggtcgatttt caaaccagaa gaactacgac                  50


<210>  172
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer to amplify p300core 

<400>  172
tatcaagctt gcatgcctgc aggttagtcc tggctctgcg tgtgcagctc                  50


<210>  173
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sgRNA-F for synthesize 72-bp sgRNA construct

<400>  173
gtatcccttg gagaaccacc t                                                 21


<210>  174
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sgRNA-R for synthesize 72-bp sgRNA construct

<400>  174
tgctgtttcc agcttagctc t                                                 21


<210>  175
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genomic DNA sequence of 500 bp upstream and 100 bp downstream 
of Sox2 transcription start site 

<400>  175
acagagcgca gtgccgcgga tgagcgcaga aacaatggca caccacctcc ggctctgcca       60

gcttcctgaa atactagttg gacagtcgcc ctgaaccacc catgggcctt gccccaccct      120

ggccccagct tcccgcgccc catccaccct tatgtatcca agagagagcc aatattccgt      180

agcatggggg aaaggagctg tcgtcttggt gctgtttacc cacttccttc gaacaggcgt      240

gcgccgtgac ctgttgctga aaacgggggg cggggggggg ggatacaaag gttccccagc      300

ggccggctgc gggcccgcct cccccgcgcg gttcggggca cagcgctctg ctgggctcgg      360

ctcggcggcg cggcaggccc cgcccccttt catgcaaaac cctctggcga ggctgggctc      420

gggcgcagga gccggcgctc gctgattggc cgccggaaac ccatttattc cctgacagcc      480

cccatcacat ggatggttgt ctattaactt gttcaaaaaa gtatcaggag ttgtcaaggc      540

agagaagaga gtgtttgcaa aaagggaaaa gtactttgct gcctctttaa gactagggct      600


