                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  Novel denitrifying enzymes

<130>  Case 32585

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  365
<212>  PRT
<213>  Enterobacter cloacae

<400>  1

Met Ser Ala Glu Lys Leu Phe Thr Pro Leu Lys Val Gly Ala Val Thr 
1               5                   10                  15      


Ala Pro Asn Arg Val Phe Met Ala Pro Leu Thr Arg Leu Arg Ser Ile 
            20                  25                  30          


Glu Pro Gly Asp Ile Pro Thr Pro Leu Met Gly Glu Tyr Tyr Arg Gln 
        35                  40                  45              


Arg Ala Ser Ser Gly Leu Ile Ile Thr Glu Ala Thr Gln Ile Ser Ala 
    50                  55                  60                  


Gln Ala Lys Gly Tyr Ala Gly Ala Pro Gly Leu His Ser Pro Glu Gln 
65                  70                  75                  80  


Ile Ala Ala Trp Lys Lys Ile Thr Ala Gly Val His Ala Glu Glu Gly 
                85                  90                  95      


Arg Ile Ala Val Gln Leu Trp His Thr Gly Arg Ile Ser His Ser Ser 
            100                 105                 110         


Ile Gln Pro Gly Gly Gln Ala Pro Val Ser Ala Ser Ala Leu Asn Ala 
        115                 120                 125             


Asn Thr Arg Thr Ser Leu Arg Asp Glu Asn Gly Asn Ala Ile Arg Val 
    130                 135                 140                 


Asp Thr Ser Thr Pro Arg Ala Leu Glu Leu Asp Glu Ile Pro Gly Ile 
145                 150                 155                 160 


Val Asn Asp Phe Arg Gln Ala Val Ala Asn Ala Arg Glu Ala Gly Phe 
                165                 170                 175     


Asp Leu Val Glu Leu His Ser Ala His Gly Tyr Leu Leu His Gln Phe 
            180                 185                 190         


Leu Ser Pro Ser Ser Asn His Arg Thr Asp Gln Tyr Gly Gly Asn Val 
        195                 200                 205             


Glu Asn Arg Ala Arg Leu Val Leu Glu Val Val Asp Ala Val Cys Gln 
    210                 215                 220                 


Glu Trp Ser Pro Asp Arg Ile Gly Ile Arg Val Ser Pro Ile Gly Thr 
225                 230                 235                 240 


Phe Gln Asn Val Asp Asn Gly Pro Asn Glu Glu Ala Asp Ala Leu Tyr 
                245                 250                 255     


Leu Ile Glu Glu Leu Ala Lys Arg Gly Ile Ala Tyr Leu His Met Ser 
            260                 265                 270         


Glu Pro Asp Trp Ala Gly Gly Gln Pro Tyr Ser Glu Ala Phe Arg Gln 
        275                 280                 285             


Lys Val Arg Glu Arg Phe His Gly Val Ile Ile Gly Ala Gly Ala Tyr 
    290                 295                 300                 


Thr Pro Glu Lys Ala Glu Asp Leu Ile Asn Lys Gly Leu Ile Asp Ala 
305                 310                 315                 320 


Val Ala Phe Gly Arg Asp Tyr Ile Ala Asn Pro Asp Leu Val Ala Arg 
                325                 330                 335     


Leu Gln Gln Lys Ala Ala Leu Asn Pro Gln Arg Pro Glu Ser Phe Tyr 
            340                 345                 350         


Gly Gly Gly Ala Glu Gly Tyr Thr Asp Tyr Pro Thr Leu 
        355                 360                 365 


<210>  2
<211>  365
<212>  PRT
<213>  Enterobacter cloacae PB2

<400>  2

Met Ser Ala Glu Lys Leu Phe Thr Pro Leu Lys Val Gly Ala Val Thr 
1               5                   10                  15      


Ala Pro Asn Arg Val Phe Met Ala Pro Leu Thr Arg Leu Arg Ser Ile 
            20                  25                  30          


Glu Pro Gly Asp Ile Pro Thr Pro Leu Met Gly Glu Tyr Tyr Arg Gln 
        35                  40                  45              


Arg Ala Ser Ala Gly Leu Ile Ile Ser Glu Ala Thr Gln Ile Ser Ala 
    50                  55                  60                  


Gln Ala Lys Gly Tyr Ala Gly Ala Pro Gly Leu His Ser Pro Glu Gln 
65                  70                  75                  80  


Ile Ala Ala Trp Lys Lys Ile Thr Ala Gly Val His Ala Glu Asp Gly 
                85                  90                  95      


Arg Ile Ala Val Gln Leu Trp His Thr Gly Arg Ile Ser His Ser Ser 
            100                 105                 110         


Ile Gln Pro Gly Gly Gln Ala Pro Val Ser Ala Ser Ala Leu Asn Ala 
        115                 120                 125             


Asn Thr Arg Thr Ser Leu Arg Asp Glu Asn Gly Asn Ala Ile Arg Val 
    130                 135                 140                 


Asp Thr Thr Thr Pro Arg Ala Leu Glu Leu Asp Glu Ile Pro Gly Ile 
145                 150                 155                 160 


Val Asn Asp Phe Arg Gln Ala Val Ala Asn Ala Arg Glu Ala Gly Phe 
                165                 170                 175     


Asp Leu Val Glu Leu His Ser Ala His Gly Tyr Leu Leu His Gln Phe 
            180                 185                 190         


Leu Ser Pro Ser Ser Asn Gln Arg Thr Asp Gln Tyr Gly Gly Ser Val 
        195                 200                 205             


Glu Asn Arg Ala Arg Leu Val Leu Glu Val Val Asp Ala Val Cys Asn 
    210                 215                 220                 


Glu Trp Ser Ala Asp Arg Ile Gly Ile Arg Val Ser Pro Ile Gly Thr 
225                 230                 235                 240 


Phe Gln Asn Val Asp Asn Gly Pro Asn Glu Glu Ala Asp Ala Leu Tyr 
                245                 250                 255     


Leu Ile Glu Glu Leu Ala Lys Arg Gly Ile Ala Tyr Leu His Met Ser 
            260                 265                 270         


Glu Thr Asp Leu Ala Gly Gly Lys Pro Tyr Ser Glu Ala Phe Arg Gln 
        275                 280                 285             


Lys Val Arg Glu Arg Phe His Gly Val Ile Ile Gly Ala Gly Ala Tyr 
    290                 295                 300                 


Thr Ala Glu Lys Ala Glu Asp Leu Ile Gly Lys Gly Leu Ile Asp Ala 
305                 310                 315                 320 


Val Ala Phe Gly Arg Asp Tyr Ile Ala Asn Pro Asp Leu Val Ala Arg 
                325                 330                 335     


Leu Gln Lys Lys Ala Glu Leu Asn Pro Gln Arg Pro Glu Ser Phe Tyr 
            340                 345                 350         


Gly Gly Gly Ala Glu Gly Tyr Thr Asp Tyr Pro Ser Leu 
        355                 360                 365 


<210>  3
<211>  338
<212>  PRT
<213>  Bacillus subtilis

<400>  3

Met Ala Arg Lys Leu Phe Thr Pro Ile Thr Ile Lys Asp Met Thr Leu 
1               5                   10                  15      


Lys Asn Arg Ile Val Met Ser Pro Met Cys Met Tyr Ser Ser His Glu 
            20                  25                  30          


Lys Asp Gly Lys Leu Thr Pro Phe His Met Ala His Tyr Ile Ser Arg 
        35                  40                  45              


Ala Ile Gly Gln Val Gly Leu Ile Ile Val Glu Ala Ser Ala Val Asn 
    50                  55                  60                  


Pro Gln Gly Arg Ile Thr Asp Gln Asp Leu Gly Ile Trp Ser Asp Glu 
65                  70                  75                  80  


His Ile Glu Gly Phe Ala Lys Leu Thr Glu Gln Val Lys Glu Gln Gly 
                85                  90                  95      


Ser Lys Ile Gly Ile Gln Leu Ala His Ala Gly Arg Lys Ala Glu Leu 
            100                 105                 110         


Glu Gly Asp Ile Phe Ala Pro Ser Ala Ile Ala Phe Asp Glu Gln Ser 
        115                 120                 125             


Ala Thr Pro Val Glu Met Ser Ala Glu Lys Val Lys Glu Thr Val Gln 
    130                 135                 140                 


Glu Phe Lys Gln Ala Ala Ala Arg Ala Lys Glu Ala Gly Phe Asp Val 
145                 150                 155                 160 


Ile Glu Ile His Ala Ala His Gly Tyr Leu Ile His Glu Phe Leu Ser 
                165                 170                 175     


Pro Leu Ser Asn His Arg Thr Asp Glu Tyr Gly Gly Ser Pro Glu Asn 
            180                 185                 190         


Arg Tyr Arg Phe Leu Arg Glu Ile Ile Asp Glu Val Lys Gln Val Trp 
        195                 200                 205             


Asp Gly Pro Leu Phe Val Arg Val Ser Ala Ser Asp Tyr Thr Asp Lys 
    210                 215                 220                 


Gly Leu Asp Ile Ala Asp His Ile Gly Phe Ala Lys Trp Met Lys Glu 
225                 230                 235                 240 


Gln Gly Val Asp Leu Ile Asp Cys Ser Ser Gly Ala Leu Val His Ala 
                245                 250                 255     


Asp Ile Asn Val Phe Pro Gly Tyr Gln Val Ser Phe Ala Glu Lys Ile 
            260                 265                 270         


Arg Glu Gln Ala Asp Met Ala Thr Gly Ala Val Gly Met Ile Thr Asp 
        275                 280                 285             


Gly Ser Met Ala Glu Glu Ile Leu Gln Asn Gly Arg Ala Asp Leu Ile 
    290                 295                 300                 


Phe Ile Gly Arg Glu Leu Leu Arg Asp Pro Phe Phe Ala Arg Thr Ala 
305                 310                 315                 320 


Ala Lys Gln Leu Asn Thr Glu Ile Pro Ala Pro Val Gln Tyr Glu Arg 
                325                 330                 335     


Gly Trp 
        


<210>  4
<211>  365
<212>  PRT
<213>  Escherichia coli JM109

<400>  4

Met Ser Ser Glu Lys Leu Tyr Ser Pro Leu Lys Val Gly Ala Ile Thr 
1               5                   10                  15      


Ala Ala Asn Arg Ile Phe Met Ala Pro Leu Thr Arg Leu Arg Ser Ile 
            20                  25                  30          


Glu Pro Gly Asp Ile Pro Thr Pro Leu Met Ala Glu Tyr Tyr Arg Gln 
        35                  40                  45              


Arg Ala Ser Ala Gly Leu Ile Ile Ser Glu Ala Thr Gln Ile Ser Ala 
    50                  55                  60                  


Gln Ala Lys Gly Tyr Ala Gly Ala Pro Gly Ile His Ser Pro Glu Gln 
65                  70                  75                  80  


Ile Ala Ala Trp Lys Lys Ile Thr Ala Gly Val His Ala Glu Asn Gly 
                85                  90                  95      


His Met Ala Val Gln Leu Trp His Thr Gly Arg Ile Ser His Ala Ser 
            100                 105                 110         


Leu Gln Pro Gly Gly Gln Ala Pro Val Ala Pro Ser Ala Leu Ser Ala 
        115                 120                 125             


Gly Thr Arg Thr Ser Leu Arg Asp Glu Asn Gly Gln Ala Ile Arg Val 
    130                 135                 140                 


Glu Thr Ser Met Pro Arg Ala Leu Glu Leu Glu Glu Ile Pro Gly Ile 
145                 150                 155                 160 


Val Asn Asp Phe Arg Gln Ala Ile Ala Asn Ala Arg Glu Ala Gly Phe 
                165                 170                 175     


Asp Leu Val Glu Leu His Ser Ala His Gly Tyr Leu Leu His Gln Phe 
            180                 185                 190         


Leu Ser Pro Ser Ser Asn His Arg Thr Asp Gln Tyr Gly Gly Ser Val 
        195                 200                 205             


Glu Asn Arg Ala Arg Leu Val Leu Glu Val Val Asp Ala Gly Ile Glu 
    210                 215                 220                 


Glu Trp Gly Ala Asp Arg Ile Gly Ile Arg Val Ser Pro Ile Gly Thr 
225                 230                 235                 240 


Phe Gln Asn Thr Asp Asn Gly Pro Asn Glu Glu Ala Asp Ala Leu Tyr 
                245                 250                 255     


Leu Ile Glu Gln Leu Gly Lys Arg Gly Ile Ala Tyr Leu His Met Ser 
            260                 265                 270         


Glu Pro Asp Trp Ala Gly Gly Glu Pro Tyr Thr Asp Ala Phe Arg Glu 
        275                 280                 285             


Lys Val Arg Ala Arg Phe His Gly Pro Ile Ile Gly Ala Gly Ala Tyr 
    290                 295                 300                 


Thr Val Glu Lys Ala Glu Thr Leu Ile Gly Lys Gly Leu Ile Asp Ala 
305                 310                 315                 320 


Val Ala Phe Gly Arg Asp Trp Ile Ala Asn Pro Asp Leu Val Ala Arg 
                325                 330                 335     


Leu Gln Arg Lys Ala Glu Leu Asn Pro Gln Arg Ala Glu Ser Phe Tyr 
            340                 345                 350         


Gly Gly Gly Ala Glu Gly Tyr Thr Asp Tyr Pro Thr Leu 
        355                 360                 365 


<210>  5
<211>  349
<212>  PRT
<213>  Pseudomonas fluorescens I-C

<400>  5

Met Ala Thr Ile Phe Asp Pro Ile Lys Leu Gly Asp Leu Glu Leu Ser 
1               5                   10                  15      


Asn Arg Ile Ile Met Ala Pro Leu Thr Arg Cys Arg Ala Asp Glu Gly 
            20                  25                  30          


Arg Val Pro Asn Ala Leu Met Ala Glu Tyr Tyr Val Gln Arg Ala Ser 
        35                  40                  45              


Ala Gly Leu Ile Leu Ser Glu Ala Thr Ser Val Thr Pro Met Gly Val 
    50                  55                  60                  


Gly Tyr Pro Asp Thr Pro Gly Ile Trp Ser Asn Asp Gln Val Arg Gly 
65                  70                  75                  80  


Trp Thr Asn Ile Thr Lys Ala Val His Ala Ala Gly Gly Lys Ile Val 
                85                  90                  95      


Leu Gln Leu Trp His Val Gly Arg Ile Ser His Pro Leu Tyr Leu Asn 
            100                 105                 110         


Gly Glu Ala Pro Val Ala Pro Ser Ala Ile Gln Pro Lys Gly His Val 
        115                 120                 125             


Ser Leu Val Arg Pro Leu Ala Asp Tyr Pro Thr Pro Arg Ala Leu Glu 
    130                 135                 140                 


Thr Ala Glu Ile Ala Glu Ile Val Glu Ala Tyr Arg Thr Gly Ala Glu 
145                 150                 155                 160 


Asn Ala Lys Ala Ala Gly Phe Asp Gly Val Glu Ile His Gly Ala Asn 
                165                 170                 175     


Gly Tyr Leu Leu Asp Gln Phe Leu Gln Ser Ser Thr Asn Gln Arg Thr 
            180                 185                 190         


Asp Asn Tyr Gly Gly Ser Leu Glu Asn Arg Ala Arg Leu Leu Leu Glu 
        195                 200                 205             


Val Thr Asp Ala Ala Ile Asp Val Trp Gly Ala Gly Arg Val Gly Val 
    210                 215                 220                 


His Leu Ala Pro Arg Ala Asp Ser His Asp Met Gly Asp Asp Asn Leu 
225                 230                 235                 240 


Ala Glu Thr Phe Thr Tyr Val Ala Arg Glu Leu Gly Lys Arg Gly Ile 
                245                 250                 255     


Ala Phe Ile Cys Ser Arg Glu Lys Glu Gly Ala Asp Ser Leu Gly Pro 
            260                 265                 270         


Gln Leu Lys Glu Ala Phe Gly Gly Ala Tyr Ile Ala Asn Glu Arg Phe 
        275                 280                 285             


Thr Lys Asp Ser Ala Asn Ala Trp Leu Ala Glu Gly Lys Ala Asp Ala 
    290                 295                 300                 


Val Ala Phe Gly Val Pro Phe Ile Ala Asn Pro Asp Leu Pro Ala Arg 
305                 310                 315                 320 


Leu Lys Ala Asp Ala Pro Leu Asn Glu Pro Arg Pro Glu Leu Phe Tyr 
                325                 330                 335     


Gly Lys Gly Pro Val Gly Tyr Ile Asp Tyr Pro Thr Leu 
            340                 345                 


<210>  6
<211>  398
<212>  PRT
<213>  Kluyveromyces lactis

<400>  6

Met Ser Phe Met Asn Phe Glu Pro Lys Pro Leu Ala Asp Thr Asp Ile 
1               5                   10                  15      


Phe Lys Pro Ile Lys Ile Gly Asn Thr Glu Leu Lys His Arg Val Val 
            20                  25                  30          


Met Pro Ala Leu Thr Arg Met Arg Ala Leu His Pro Gly Asn Val Pro 
        35                  40                  45              


Asn Pro Asp Trp Ala Val Glu Tyr Tyr Arg Gln Arg Ser Gln Tyr Pro 
    50                  55                  60                  


Gly Thr Met Ile Ile Thr Glu Gly Ala Phe Pro Ser Ala Gln Ser Gly 
65                  70                  75                  80  


Gly Tyr Asp Asn Ala Pro Gly Val Trp Ser Glu Glu Gln Leu Ala Gln 
                85                  90                  95      


Trp Arg Lys Ile Phe Lys Ala Ile His Asp Asn Lys Ser Phe Val Trp 
            100                 105                 110         


Val Gln Leu Trp Val Leu Gly Arg Gln Ala Phe Ala Asp Asn Leu Ala 
        115                 120                 125             


Arg Asp Gly Leu Arg Tyr Asp Ser Ala Ser Asp Glu Val Tyr Met Gly 
    130                 135                 140                 


Glu Asp Glu Lys Glu Arg Ala Ile Arg Ser Asn Asn Pro Gln His Gly 
145                 150                 155                 160 


Ile Thr Lys Asp Glu Ile Lys Gln Tyr Ile Arg Asp Tyr Val Asp Ala 
                165                 170                 175     


Ala Lys Lys Cys Ile Asp Ala Gly Ala Asp Gly Val Glu Ile His Ser 
            180                 185                 190         


Ala Asn Gly Tyr Leu Leu Asn Gln Phe Leu Asp Pro Ile Ser Asn Lys 
        195                 200                 205             


Arg Thr Asp Glu Tyr Gly Gly Ser Ile Glu Asn Arg Ala Arg Phe Val 
    210                 215                 220                 


Leu Glu Val Val Asp Ala Val Val Asp Ala Val Gly Ala Glu Arg Thr 
225                 230                 235                 240 


Ser Ile Arg Phe Ser Pro Tyr Gly Val Phe Gly Thr Met Ser Gly Val 
                245                 250                 255     


Ser Asp Pro Val Leu Val Ala Gln Phe Ala Tyr Val Leu Ala Glu Leu 
            260                 265                 270         


Glu Lys Arg Ala Lys Ala Gly Lys Arg Leu Ala Tyr Val Asp Leu Val 
        275                 280                 285             


Glu Pro Arg Val Thr Ser Pro Phe Gln Pro Glu Phe Glu Gly Trp Tyr 
    290                 295                 300                 


Lys Gly Gly Thr Asn Glu Phe Val Tyr Ser Val Trp Lys Gly Asn Val 
305                 310                 315                 320 


Leu Arg Val Gly Asn Tyr Ala Leu Asp Pro Asp Ala Ala Ile Thr Asp 
                325                 330                 335     


Ser Lys Asn Pro Asn Thr Leu Ile Gly Tyr Gly Arg Ala Phe Ile Ala 
            340                 345                 350         


Asn Pro Asp Leu Val Glu Arg Leu Glu Lys Gly Leu Pro Leu Asn Gln 
        355                 360                 365             


Tyr Asp Arg Pro Ser Phe Tyr Lys Met Ser Ala Glu Gly Tyr Ile Asp 
    370                 375                 380                 


Tyr Pro Thr Tyr Glu Glu Ala Val Ala Lys Gly Tyr Lys Lys 
385                 390                 395             


