﻿               SEQUENCE LISTING

<110> UNIVERSIDAD DE CHILE

<120> Anticuerpo humano y fragmentos de este, para ser utilizados en el tratamiento del cáncer gástrico (CG) y eventualmente otro tipo de tumores que expresen la proteína MICA

<130> 273146-CL

<160> 17

<170> BiSSAP 1.3.6

<210> 1
<211> 20
<212> PRT
<213> Homo sapiens


<400> 1
Arg Asp Leu Thr Gly Asn Gly Lys Asp Leu Arg Met Thr Leu Ala His 
1               5                   10                  15      
Ile Lys Asp Gln 
            20  

<210> 2
<211> 132
<212> PRT
<213> Homo sapiens


<400> 2
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser Val Arg Ser Ala 
            20                  25                  30          
Tyr Ser Ser Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu 
        35                  40                  45              
Trp Ile Gly Gln Ile His Pro Asn Gly Asn Thr Tyr Tyr Asn Pro Ser 
    50                  55                  60                  
Leu Met Ser Arg Ile Thr Val Ser Ile Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  
Ser Leu Lys Leu Asp Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Phe 
                85                  90                  95      
Cys Ala Thr Glu Gly Val Thr Ser Glu Ser Gly Ser Phe Asp Ile Trp 
            100                 105                 110         
Gly Gln Gly Arg Met Val Ile Val Ser Ser Ala Ser Thr Lys Gly Pro 
        115                 120                 125             
Ser Val Ala Ser 
    130         

<210> 3
<211> 114
<212> PRT
<213> Homo sapiens


<400> 3
Glu Phe Gly Val Val Met Thr Gln Ser Pro Asp Phe Gln Ser Val Thr 
1               5                   10                  15      
Pro Lys Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Gly 
            20                  25                  30          
Arg Ser Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu 
        35                  40                  45              
Leu Val Met Tyr Ala Ser Gln Ser Phe Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu 
65                  70                  75                  80  
Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gln Ser Tyr Ser Val 
                85                  90                  95      
Pro Arg Ser Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val 
            100                 105                 110         
Ala Ala 
        

<210> 4
<211> 342
<212> DNA
<213> Homo sapiens


<400> 4
gaattcggtg ttgtgatgac tcagtctcca gattttcagt ctgtgactcc aaaggagaag      60

gtcaccatca cctgccgggc cagtcagagc attggtcgta gtatacactg gtaccagcag     120

aaaccagatc agtctccaaa gctcctcgtc atgtatgctt cccagtcctt ctcaggggtc     180

ccctcgaggt tcagtggcag tggatctggg acagatttca ccctcaccat caatagcctg     240

gaagctgaag atgctgcaac gtattactgc catcagagtt atagtgtacc tcggagtttt     300

ggccagggga ccaagctgga gatcaaacga actgtggctg ca                        342


<210> 5
<211> 396
<212> DNA
<213> Homo sapiens


<400> 5
caggtgcagc tgcaggagtc gggcccagga ctggtgaagc cttcacagac cctgtccctc      60

acctgcactg tctctggtga ctccgtcaga agtgcctatt cctcctggaa ctggatccgg     120

cagccaccag ggaagggcct ggagtggatt gggcagattc atcctaatgg gaacacctac     180

tacaacccgt ccctcatgag tcgaatcacc gtgtctatag acacgtccaa gaaccagttt     240

tccctgaagc tggactctgt gaccgccgcg gacacggccg tttatttctg tgccactgag     300

ggtgttacct ccgagtcggg ctcttttgat atctggggcc aaggcagaat ggtcatcgtc     360

tcttcagctt ccaccaaggg cccatcggtc gctagc                               396


<210> 6
<211> 11
<212> PRT
<213> Homo sapiens


<400> 6
Arg Ala Ser Gln Ser Ile Gly Arg Ser Ile His 
1               5                   10      

<210> 7
<211> 7
<212> PRT
<213> Homo sapiens


<400> 7
Met Tyr Ala Ser Gln Ser Phe 
1               5           

<210> 8
<211> 9
<212> PRT
<213> Homo sapiens


<400> 8
His Gln Ser Tyr Ser Val Pro Arg Ser 
1               5                   

<210> 9
<211> 13
<212> PRT
<213> Homo sapiens


<400> 9
Gly Asp Ser Val Arg Ser Ala Tyr Ala Ser Ser Trp Asn 
1               5                   10              

<210> 10
<211> 16
<212> PRT
<213> Homo sapiens


<400> 10
Gly Ile His Pro Asn Gly Asn Thr Tyr Tyr Asn Pro Ser Leu Met Ser 
1               5                   10                  15      



<210> 11
<211> 12
<212> PRT
<213> Homo sapiens


<400> 11
Glu Gly Val Thr Ser Glu Ser Gly Ser Phe Asp Ile 
1               5                   10          

<210> 12
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker sequence

<400> 12
Gly Gly Ser Ser Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      
Gly Gly 
        

<210> 13
<211> 17
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker sequence

<400> 13
Gly Gly Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Gly 
1               5                   10                  15      
Gly 
    

<210> 14
<211> 264
<212> PRT
<213> Artificial Sequence
<220> Unsure
<223> Linker sequence


<400> 14
Glu Phe Gly Val Val Met Thr Gln Ser Pro Asp Phe Gln Ser Val Thr 
1               5                   10                  15      
Pro Lys Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Gly 
            20                  25                  30          
Arg Ser Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu 
        35                  40                  45              
Leu Val Met Tyr Ala Ser Gln Ser Phe Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu 
65                  70                  75                  80  
Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gln Ser Tyr Ser Val 
                85                  90                  95      
Pro Arg Ser Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val 
            100                 105                 110         
Ala Ala Gly Gly Ser Ser Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             
Gly Gly Gly Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
    130                 135                 140                 
Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser 
145                 150                 155                 160 
Val Arg Ser Ala Tyr Ser Ser Trp Asn Trp Ile Arg Gln Pro Pro Gly 
                165                 170                 175     
Lys Gly Leu Glu Trp Ile Gly Gln Ile His Pro Asn Gly Asn Thr Tyr 
            180                 185                 190         
Tyr Asn Pro Ser Leu Met Ser Arg Ile Thr Val Ser Ile Asp Thr Ser 
        195                 200                 205             
Lys Asn Gln Phe Ser Leu Lys Leu Asp Ser Val Thr Ala Ala Asp Thr 
    210                 215                 220                 
Ala Val Tyr Phe Cys Ala Thr Glu Gly Val Thr Ser Glu Ser Gly Ser 
225                 230                 235                 240 
Phe Asp Ile Trp Gly Gln Gly Arg Met Val Ile Val Ser Ser Ala Ser 
                245                 250                 255     
Thr Lys Gly Pro Ser Val Ala Ser 
            260                 

<210> 15
<211> 496
<212> PRT
<213> Artificial Sequence
<220> Unsure
<223> Linker sequence


<400> 15
Glu Phe Gly Val Val Met Thr Gln Ser Pro Asp Phe Gln Ser Val Thr 
1               5                   10                  15      
Pro Lys Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Gly 
            20                  25                  30          
Arg Ser Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu 
        35                  40                  45              
Leu Val Met Tyr Ala Ser Gln Ser Phe Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu 
65                  70                  75                  80  
Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gln Ser Tyr Ser Val 
                85                  90                  95      
Pro Arg Ser Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val 
            100                 105                 110         
Ala Ala Gly Gly Ser Ser Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             
Gly Gly Gly Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
    130                 135                 140                 
Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser 
145                 150                 155                 160 
Val Arg Ser Ala Tyr Ser Ser Trp Asn Trp Ile Arg Gln Pro Pro Gly 
                165                 170                 175     
Lys Gly Leu Glu Trp Ile Gly Gln Ile His Pro Asn Gly Asn Thr Tyr 
            180                 185                 190         
Tyr Asn Pro Ser Leu Met Ser Arg Ile Thr Val Ser Ile Asp Thr Ser 
        195                 200                 205             
Lys Asn Gln Phe Ser Leu Lys Leu Asp Ser Val Thr Ala Ala Asp Thr 
    210                 215                 220                 
Ala Val Tyr Phe Cys Ala Thr Glu Gly Val Thr Ser Glu Ser Gly Ser 
225                 230                 235                 240 
Phe Asp Ile Trp Gly Gln Gly Arg Met Val Ile Val Ser Ser Ala Ser 
                245                 250                 255     
Thr Lys Gly Pro Ser Val Ala Ser Glu Pro Lys Ser Cys Asp Lys Thr 
            260                 265                 270         
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
        275                 280                 285             
Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
    290                 295                 300                 
Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
305                 310                 315                 320 
Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
                325                 330                 335     
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
            340                 345                 350         
Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
        355                 360                 365             
Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
    370                 375                 380                 
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
385                 390                 395                 400 
Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 
                405                 410                 415     
Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
            420                 425                 430         
Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
        435                 440                 445             
Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
    450                 455                 460                 
Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
465                 470                 475                 480 
Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                485                 490                 495     



<210> 16
<211> 216
<212> PRT
<213> Artificial Sequence
<220> Unsure
<223> Linker sequence


<400> 16
Glu Phe Gly Val Val Met Thr Gln Ser Pro Asp Phe Gln Ser Val Thr 
1               5                   10                  15      
Pro Lys Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Gly 
            20                  25                  30          
Arg Ser Ile His Trp Tyr Gln Gln Lys Pro Asp Gln Ser Pro Lys Leu 
        35                  40                  45              
Leu Val Met Tyr Ala Ser Gln Ser Phe Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  
Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn Ser Leu 
65                  70                  75                  80  
Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys His Gln Ser Tyr Ser Val 
                85                  90                  95      
Pro Arg Ser Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val 
            100                 105                 110         
Ala Ala Ala Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
        115                 120                 125             
Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
    130                 135                 140                 
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
145                 150                 155                 160 
Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
                165                 170                 175     
Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
            180                 185                 190         
Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
        195                 200                 205             
Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215     

<210> 17
<211> 452
<212> PRT
<213> Artificial Sequence
<220> Unsure
<223> Linker sequence


<400> 17
Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      
Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Asp Ser Val Arg Ser Ala 
            20                  25                  30          
Tyr Ser Ser Trp Asn Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu 
        35                  40                  45              
Trp Ile Gly Gln Ile His Pro Asn Gly Asn Thr Tyr Tyr Asn Pro Ser 
    50                  55                  60                  
Leu Met Ser Arg Ile Thr Val Ser Ile Asp Thr Ser Lys Asn Gln Phe 
65                  70                  75                  80  
Ser Leu Lys Leu Asp Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Phe 
                85                  90                  95      
Cys Ala Thr Glu Gly Val Thr Ser Glu Ser Gly Ser Phe Asp Ile Trp 
            100                 105                 110         
Gly Gln Gly Arg Met Val Ile Val Ser Ser Ala Ser Thr Lys Gly Pro 
        115                 120                 125             
Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
    130                 135                 140                 
Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
145                 150                 155                 160 
Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
                165                 170                 175     
Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
            180                 185                 190         
Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
        195                 200                 205             
His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser 
    210                 215                 220                 
Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
225                 230                 235                 240 
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                245                 250                 255     
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            260                 265                 270         
His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        275                 280                 285             
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    290                 295                 300                 
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
305                 310                 315                 320 
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                325                 330                 335     
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            340                 345                 350         
Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
        355                 360                 365             
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    370                 375                 380                 
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
385                 390                 395                 400 
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
                405                 410                 415     
Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            420                 425                 430         
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        435                 440                 445             
Ser Pro Gly Lys 
    450         

