                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Jarjour, Jordan
       Havens, Kyle
 
<120>  CTLA4 HOMING ENDONUCLEASE VARIANTS, COMPOSITIONS, AND METHODS OF 
       USE

<130>  BLBD-094/01WO 315698-2777

<150>  US 62/608,397
<151>  2017-12-20

<160>  70    

<170>  PatentIn version 3.5

<210>  1
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  1

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Met Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Cys Val Met Glu Asn Lys Glu His Leu Lys Ile Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Ile Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  2
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  2

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  3
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(3)
<223>  Any amino acid or absent

<400>  3

Xaa Xaa Xaa Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  4
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  4

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  5
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  5

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  6
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant CTLA4.B3


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  6

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe His Leu Ser Ile Arg Asn Ser 
            20                  25                  30          


Asn Gly Tyr Arg Ser Gly Tyr Lys Thr Ser Leu Ser Phe Ser Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Thr Ile Ala Asn His Gly Asp His Ala Val Ile Leu Thr 
65                  70                  75                  80  


Val Arg Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Ile Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Gly Val Glu Leu Val Lys Arg Ser Arg His Gly 
            180                 185                 190         


Lys Val Gly Val Arg Leu Arg Phe Ser Ile Gly Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Asp Glu Arg Asn Ser Ser Glu Lys Ser Trp Leu Glu Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  7
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant CTLA4.B3.B6


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  7

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe His Leu Ser Ile Arg Asn Asn 
            20                  25                  30          


Asn Gly Tyr Arg Ser Gly Tyr Lys Thr Ser Leu Ser Phe Ser Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Thr Ile Ala Asn His Gly Asp His Ala Val Ile Leu Thr 
65                  70                  75                  80  


Val Arg Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Ile Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Gly Val Glu Leu Val Lys Arg Ser Arg His Gly 
            180                 185                 190         


Lys Val Gly Val Arg Leu Arg Phe Ser Ile Gly Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Asp Glu Arg Asn Ser Ser Glu Lys Ser Trp Leu Glu Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  8
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant CTLA4.B3.B6.D5


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  8

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe His Leu Ser Ile Arg Asn Asn 
            20                  25                  30          


Asn Gly Tyr Arg Ser Gly Tyr Lys Thr Ser Leu Ser Phe Ser Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Thr Ile Ala Asn His Gly Asp His Ala Val Ile Leu Thr 
65                  70                  75                  80  


Val Arg Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Ile Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Gly Val Glu Leu Val Lys Arg Ser Arg His Gly 
            180                 185                 190         


Lys Val Gly Val Arg Leu Arg Phe Ser Ile Gly Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Asp Glu Arg Asn Ser Ser Glu Arg Ser Trp Leu Glu Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  9
<211>  808
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL CTLA4.B3 construct

<400>  9

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln Leu Ser 
        435                 440                 445             


Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala 
    450                 455                 460                 


Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys Lys Gly 
465                 470                 475                 480 


Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg Ile Gly 
                485                 490                 495     


Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly Gly Ser Ser 
            500                 505                 510         


Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala 
        515                 520                 525             


Glu Gly Ser Phe His Leu Ser Ile Arg Asn Ser Asn Gly Tyr Arg Ser 
    530                 535                 540                 


Gly Tyr Lys Thr Ser Leu Ser Phe Ser Ile Thr Leu His Asn Lys Asp 
545                 550                 555                 560 


Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Thr Ile 
                565                 570                 575     


Ala Asn His Gly Asp His Ala Val Ile Leu Thr Val Arg Arg Phe Glu 
            580                 585                 590         


Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr 
        595                 600                 605             


Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Ile 
    610                 615                 620                 


Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg 
625                 630                 635                 640 


Ile Lys Ala Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala 
                645                 650                 655     


Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile 
            660                 665                 670         


Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe 
        675                 680                 685             


Gly Val Glu Leu Val Lys Arg Ser Arg His Gly Lys Val Gly Val Arg 
    690                 695                 700                 


Leu Arg Phe Ser Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn 
705                 710                 715                 720 


Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Asp Glu Arg Asn Ser 
                725                 730                 735     


Ser Glu Lys Ser Trp Leu Glu Phe Arg Val Thr Lys Phe Ser Asp Ile 
            740                 745                 750         


Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val 
        755                 760                 765             


Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu 
    770                 775                 780                 


Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile 
785                 790                 795                 800 


Lys Leu Asn Met Asn Lys Gly Arg 
                805             


<210>  10
<211>  808
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL CTLA4.B3.B6 construct

<400>  10

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln Leu Ser 
        435                 440                 445             


Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala 
    450                 455                 460                 


Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys Lys Gly 
465                 470                 475                 480 


Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg Ile Gly 
                485                 490                 495     


Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly Gly Ser Ser 
            500                 505                 510         


Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala 
        515                 520                 525             


Glu Gly Ser Phe His Leu Ser Ile Arg Asn Asn Asn Gly Tyr Arg Ser 
    530                 535                 540                 


Gly Tyr Lys Thr Ser Leu Ser Phe Ser Ile Thr Leu His Asn Lys Asp 
545                 550                 555                 560 


Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Thr Ile 
                565                 570                 575     


Ala Asn His Gly Asp His Ala Val Ile Leu Thr Val Arg Arg Phe Glu 
            580                 585                 590         


Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr 
        595                 600                 605             


Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Ile 
    610                 615                 620                 


Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg 
625                 630                 635                 640 


Ile Lys Ala Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala 
                645                 650                 655     


Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile 
            660                 665                 670         


Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe 
        675                 680                 685             


Gly Val Glu Leu Val Lys Arg Ser Arg His Gly Lys Val Gly Val Arg 
    690                 695                 700                 


Leu Arg Phe Ser Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn 
705                 710                 715                 720 


Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Asp Glu Arg Asn Ser 
                725                 730                 735     


Ser Glu Lys Ser Trp Leu Glu Phe Arg Val Thr Lys Phe Ser Asp Ile 
            740                 745                 750         


Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val 
        755                 760                 765             


Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu 
    770                 775                 780                 


Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile 
785                 790                 795                 800 


Lys Leu Asn Met Asn Lys Gly Arg 
                805             


<210>  11
<211>  808
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL CTLA4.B3.B6.D5 construct

<400>  11

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln Leu Ser 
        435                 440                 445             


Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala 
    450                 455                 460                 


Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys Lys Gly 
465                 470                 475                 480 


Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg Ile Gly 
                485                 490                 495     


Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly Gly Ser Ser 
            500                 505                 510         


Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala 
        515                 520                 525             


Glu Gly Ser Phe His Leu Ser Ile Arg Asn Asn Asn Gly Tyr Arg Ser 
    530                 535                 540                 


Gly Tyr Lys Thr Ser Leu Ser Phe Ser Ile Thr Leu His Asn Lys Asp 
545                 550                 555                 560 


Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Thr Ile 
                565                 570                 575     


Ala Asn His Gly Asp His Ala Val Ile Leu Thr Val Arg Arg Phe Glu 
            580                 585                 590         


Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr 
        595                 600                 605             


Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Ile 
    610                 615                 620                 


Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg 
625                 630                 635                 640 


Ile Lys Ala Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala 
                645                 650                 655     


Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile 
            660                 665                 670         


Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe 
        675                 680                 685             


Gly Val Glu Leu Val Lys Arg Ser Arg His Gly Lys Val Gly Val Arg 
    690                 695                 700                 


Leu Arg Phe Ser Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn 
705                 710                 715                 720 


Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Asp Glu Arg Asn Ser 
                725                 730                 735     


Ser Glu Arg Ser Trp Leu Glu Phe Arg Val Thr Lys Phe Ser Asp Ile 
            740                 745                 750         


Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val 
        755                 760                 765             


Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu 
    770                 775                 780                 


Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile 
785                 790                 795                 800 


Lys Leu Asn Met Asn Lys Gly Arg 
                805             


<210>  12
<211>  1049
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL CTLA4.B3.B6.D5- TREX2 fusion

<400>  12

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala Gln Leu Ser 
        435                 440                 445             


Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala 
    450                 455                 460                 


Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val Lys Lys Gly 
465                 470                 475                 480 


Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg Arg Ile Gly 
                485                 490                 495     


Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly Gly Ser Ser 
            500                 505                 510         


Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala 
        515                 520                 525             


Glu Gly Ser Phe His Leu Ser Ile Arg Asn Asn Asn Gly Tyr Arg Ser 
    530                 535                 540                 


Gly Tyr Lys Thr Ser Leu Ser Phe Ser Ile Thr Leu His Asn Lys Asp 
545                 550                 555                 560 


Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Thr Ile 
                565                 570                 575     


Ala Asn His Gly Asp His Ala Val Ile Leu Thr Val Arg Arg Phe Glu 
            580                 585                 590         


Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr 
        595                 600                 605             


Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Ile 
    610                 615                 620                 


Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg 
625                 630                 635                 640 


Ile Lys Ala Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala 
                645                 650                 655     


Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile 
            660                 665                 670         


Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe 
        675                 680                 685             


Gly Val Glu Leu Val Lys Arg Ser Arg His Gly Lys Val Gly Val Arg 
    690                 695                 700                 


Leu Arg Phe Ser Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn 
705                 710                 715                 720 


Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Asp Glu Arg Asn Ser 
                725                 730                 735     


Ser Glu Arg Ser Trp Leu Glu Phe Arg Val Thr Lys Phe Ser Asp Ile 
            740                 745                 750         


Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val 
        755                 760                 765             


Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu 
    770                 775                 780                 


Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile 
785                 790                 795                 800 


Lys Leu Asn Met Asn Lys Gly Arg Val Phe Ala Ser Thr Gly Ser Glu 
                805                 810                 815     


Pro Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu Ala Thr Gly 
            820                 825                 830         


Leu Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu Phe Ala Val 
        835                 840                 845             


His Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser Gly Ser Leu 
    850                 855                 860                 


Val Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met Cys Pro Glu 
865                 870                 875                 880 


Arg Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu Ser Ser Glu 
                885                 890                 895     


Ser Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala Val Val Arg 
            900                 905                 910         


Thr Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile Cys Leu Val 
        915                 920                 925             


Ala His Asn Gly Phe Asp Tyr Asp Phe Pro Leu Leu Cys Thr Glu Leu 
    930                 935                 940                 


Gln Arg Leu Gly Ala His Leu Pro Gln Asp Thr Val Cys Leu Asp Thr 
945                 950                 955                 960 


Leu Pro Ala Leu Arg Gly Leu Asp Arg Ala His Ser His Gly Thr Arg 
                965                 970                 975     


Ala Gln Gly Arg Lys Ser Tyr Ser Leu Ala Ser Leu Phe His Arg Tyr 
            980                 985                 990         


Phe Gln Ala Glu Pro Ser Ala Ala  His Ser Ala Glu Gly  Asp Val His 
        995                 1000                 1005             


Thr Leu  Leu Leu Ile Phe Leu  His Arg Ala Pro Glu  Leu Leu Ala 
    1010                 1015                 1020             


Trp Ala  Asp Glu Gln Ala Arg  Ser Trp Ala His Ile  Glu Pro Met 
    1025                 1030                 1035             


Tyr Val  Pro Pro Asp Gly Pro  Ser Leu Glu Ala 
    1040                 1045                 


<210>  13
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  13
tggagatgca tactcacaca ca                                                22


<210>  14
<211>  9
<212>  DNA
<213>  Homo sapiens

<400>  14
cagtggctt                                                                9


<210>  15
<211>  35
<212>  DNA
<213>  Homo sapiens

<400>  15
cagtggcttt gcctggagat gcatactcac acaca                                  35


<210>  16
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  16
tggagatgca tactacgtct gc                                                22


<210>  17
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  17
cttccaggaa tactcacaca ca                                                22


<210>  18
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  18
tgtctgccta tactcacaca ca                                                22


<210>  19
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  19
tttccactta tactcacaca ca                                                22


<210>  20
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  20
gccggaaaaa tactcacaca ca                                                22


<210>  21
<211>  7244
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in lab construct - I-OnuI variant CTLA4.B3.B6.D5 surface 
       display plasmid

<400>  21
gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt       60

cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata      120

atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt      180

aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa      240

atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata      300

gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct      360

tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata      420

aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact      480

attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa      540

atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg      600

ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg      660

ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt      720

attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt      780

gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg      840

ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa      900

cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt      960

gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag     1020

tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt     1080

gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga     1140

ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg ccttgatcgt     1200

tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta     1260

gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg     1320

caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc     1380

cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt     1440

atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg     1500

ggcagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg     1560

attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa     1620

cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa     1680

atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga     1740

tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg     1800

ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact     1860

ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac     1920

cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg     1980

gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg     2040

gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga     2100

acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc     2160

gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg     2220

agggagcttc caggggggaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc     2280

tgacttgagc gtcgattttt gtgatgctcg tcaggggggc cgagcctatg gaaaaacgcc     2340

agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt     2400

cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc     2460

gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc     2520

ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac     2580

aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact     2640

cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg     2700

agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt     2760

aaccctcact aaagggaaca aaagctgggt acccgacagg ttatcagcaa caacacagtc     2820

atatccattc tcaattagct ctaccacagt gtgtgaacca atgtatccag caccacctgt     2880

aaccaaaaca attttagaag tactttcact ttgtaactga gctgtcattt atattgaatt     2940

ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat     3000

ggcattacca ccatatacat atccatatac atatccatat ctaatcttac ttatatgttg     3060

tggaaatgta aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag     3120

taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggtg     3180

acagccctcc gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg     3240

aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct     3300

tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac     3360

gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg     3420

taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg     3480

cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg     3540

taataaaaga tcgaatccta cttcatacat tttcaattaa gatgcagtta cttcgctgtt     3600

tttcaatatt ttctgttatt gcttcagttt tagcacagga actgacaact atatgcgagc     3660

aaatcccctc accaacttta gaatcgacgc cgtactcttt gtcaacgact actattttgg     3720

ccaacgggaa ggcaatgcaa ggagtttttg aatattacaa atcagtaacg tttgtcagta     3780

attgcggttc tcacccctca acaactagca aaggcagccc cataaacaca cagtatgttt     3840

ttaaggacaa tagctcgacg attgaaggta gatacccata cgacgttcca gactacgctc     3900

tgcaggctag tggtggagga ggctctggtg gaggcggtag cggaggcgga gggtcggcta     3960

gctccatcaa cccatggatt ctgactggtt tcgctgatgc cgaaggatcc ttccacctat     4020

ccatcaggaa caacaacggg tatagatcag ggtacaaaac ttcgctgagc ttctcaatca     4080

cactgcacaa taaggacaaa tcgattctgg agaatatcca gtcgacttgg aaggtcggca     4140

caatcgccaa ccacggcgac cacgcagtca ttctgacggt cagacgtttc gaagatttga     4200

aagtgattat cgaccacttc gagaaatatc cgctgataac acagaaattg ggcgattaca     4260

agttgtttaa acaggcattc agcgtcatag agaacaaaga acatcttaag gagaatggga     4320

ttaaggagct cgtacgaatc aaagctaaga tgaattgggg tctcactgac gaattgaaaa     4380

aagcatttcc agagaacatt agcaaagagc gcccccttat caataagaac attccgaatc     4440

tcaaatggct ggctggattc acatctggtg acggctcgtt cggggtggaa ctagtcaaga     4500

gatcaagaca tggaaaggta ggagtgagac tgagattctc aatcggccag cacatcagag     4560

acaagaacct gatgaattca ttgataacat acctaggctg tggttacatc gacgagcgaa     4620

acagctctga gagaagttgg ctcgaattca gagtaacaaa attcagcgat atcaacgaca     4680

agatcattcc ggtattccag gaaaatactc tgattggcgt caaactcgag gactttgaag     4740

attggtgcaa ggttgccaaa ttgatcgaag agaagaaaca cctgaccgaa tccggtttgg     4800

atgagattaa gaaaatcaag ctgaacatga acaaaggtcg tgtcttctct agaggcggtt     4860

ccagaagcgg atctggtact ggcgaacaga aactcataag cgaagaagac cttagcggga     4920

ctggagagca aaagttgatt tctgaggagg atttgtcggg aaccggggag cagaagttaa     4980

tcagtgaaga ggatctcagt ggaacgggcg aacaaaagtt gatctcggag gaagacttat     5040

aatgaagatc tgataacaac agtgtagatg taacaaaatc gactttgttc ccactgtact     5100

tttagctcgt acaaaataca atatactttt catttctccg taaacaacat gttttcccat     5160

gtaatatcct tttctatttt tcgttccgtt accaacttta cacatacttt atatagctat     5220

tcacttctat acactaaaaa actaagacaa ttttaatttt gctgcctgcc atatttcaat     5280

ttgttataaa ttcctataat ttatcctatt agtagctaaa aaaagatgaa tgtgaatcga     5340

atcctaagag aattgagctc caattcgccc tatagtgagt cgtattacaa ttcactggcc     5400

gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca     5460

gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcctttcc     5520

caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg     5580

cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc     5640

ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa     5700

atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac     5760

ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt     5820

tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca     5880

accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt     5940

taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta     6000

caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaggca     6060

agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct tagcattttt     6120

gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac ctccgcttac     6180

atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg gcgtcagtcc     6240

accagctaac ataaaatgta agctttcggg gctctcttgc cttccaaccc agtcagaaat     6300

cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa     6360

cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag     6420

tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg     6480

cagttggacg acatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg     6540

attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac     6600

aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca     6660

tataataccc agcaagtcag catcggaatc aagagcacat tctgcggcct ctgtgctctg     6720

caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa cagacatact     6780

ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc accaatgccc     6840

tccctcttgg ccctctcctt ttcttttttc gaccgaatta attcttaatc ggcaaaaaaa     6900

gaaaagctcc ggatcaagat tgtacgtaag gtgacaagct atttttcaat aaagaatatc     6960

ttccactact gccatctggc gtcataactg caaagtacac atatattacg atgctgtcta     7020

ttaaatgctt cctatattat atatatagta atgtcgttta tggtgcactc tcagtacaat     7080

ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc     7140

ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag     7200

ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga                      7244


<210>  22
<211>  2427
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL CTLA4.B3.B6.D5 mRNA construct

<400>  22
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaacuua acacccgauc aagugguagc gauagcguca      480

caugaugggg guaaacaggc uuuggagacg guacagcggu uauugccggu acucugccag      540

gaccacggau ugacaccgga ccaaguggug gcgauugcgu ccaacauugg aggcaagcag      600

gcacuagaga ccguccaacg gcuucuuccc guucuuuguc aggaucaugg gcuaaccccu      660

gaucagguag ucgcuauagc uucaaacaac gggggcaagc aagcacugga gaccguucaa      720

cgacuccugc cagugcucug ccaagaccac ggacuuacgc cagaucaggu gguugcuauu      780

gccuccaacg guggcgggaa acaagcguug gaaacugugc agagacuguu accugucuug      840

ugucaagacc acggccucac gccagaucag gugguagcca uagcgucgaa caaugguggu      900

aagcaagccc uugaaacggu ccagcgucuu cugccggugu ugugccagga ccacggacua      960

acgccggauc aggucguagc cauugcuuca aauaauggcg gcaaacaggc gcuagagaca     1020

guccagcgcc ucuugccugu guuaugccag gaucacggcu uaaccccaga ccaaguugug     1080

gcuauugcau cucacgacgg uggcaaacaa gccuuggaga cagugcaacg auuacugccu     1140

gucuuauguc aggaucaugg ccugacgccc gaucagguag uggcaaucgc aucuaacggc     1200

ggagguaagc aagcacugga gacuguccag agauuguuac ccguacuaug ucaagaucau     1260

ggucugacac cugaccaagu uguggcgaua gccaguaacg gggggggaaa acaggcacua     1320

gagagcauug uggcccagcu gagccggccu gauccggcgu uggccgcguu gaccaacgac     1380

caccucgucg ccuuggccug ccucggcgga cguccugcca uggaugcagu gaaaaaggga     1440

uugccgcacg cgccggaauu gaucagaaga gucaaucgcc guauuggcga acgcacgucc     1500

caucgcguug cgauaucuag agugggagga agcucgcgca gagaguccau caacccaugg     1560

auucugacug guuucgcuga ugccgaagga uccuuccacc uauccaucag gaacaacaac     1620

ggguauagau caggguacaa aacuucgcug agcuucucaa ucacacugca caauaaggac     1680

aaaucgauuc uggagaauau ccagucgacu uggaaggucg gcacaaucgc caaccacggc     1740

gaccacgcag ucauucugac ggucagacgu uucgaagauu ugaaagugau uaucgaccac     1800

uucgagaaau auccgcugau aacacagaaa uugggcgauu acaaguuguu uaaacaggca     1860

uucagcguca uagagaacaa agaacaucuu aaggagaaug ggauuaagga gcucguacga     1920

aucaaagcua agaugaauug gggucucacu gacgaauuga aaaaagcauu uccagagaac     1980

auuagcaaag agcgcccccu uaucaauaag aacauuccga aucucaaaug gcuggcugga     2040

uucacaucug gugacggcuc guucggggug gaacuaguca agagaucaag acauggaaag     2100

guaggaguga gacugagauu cucaaucggc cagcacauca gagacaagaa ccugaugaau     2160

ucauugauaa cauaccuagg cugugguuac aucgacgagc gaaacagcuc ugagagaagu     2220

uggcucgaau ucagaguaac aaaauucagc gauaucaacg acaagaucau uccgguauuc     2280

caggaaaaua cucugauugg cgucaaacuc gaggacuuug aagauuggug caagguugcc     2340

aaauugaucg aagagaagaa acaccugacc gaauccgguu uggaugagau uaagaaaauc     2400

aagcugaaca ugaacaaagg ucguuga                                         2427


<210>  23
<211>  711
<212>  RNA
<213>  Mus musculus

<400>  23
augucugagc caccucgggc ugagaccuuu guauuccugg accuagaagc cacugggcuc       60

ccaaacaugg acccugagau ugcagagaua ucccuuuuug cuguucaccg cucuucccug      120

gagaacccag aacgggauga uucugguucc uuggugcugc cccguguucu ggacaagcuc      180

acacugugca ugugcccgga gcgccccuuu acugccaagg ccagugagau uacugguuug      240

agcagcgaaa gccugaugca cugcgggaag gcugguuuca auggcgcugu gguaaggaca      300

cugcagggcu uccuaagccg ccaggagggc cccaucugcc uuguggccca caauggcuuc      360

gauuaugacu ucccacugcu gugcacggag cuacaacguc ugggugccca ucugccccaa      420

gacacugucu gccuggacac acugccugca uugcggggcc uggaccgugc ucacagccac      480

ggcaccaggg cucaaggccg caaaagcuac agccuggcca gucucuucca ccgcuacuuc      540

caggcugaac ccagugcugc ccauucagca gaaggugaug ugcacacccu gcuucugauc      600

uuccugcauc gugcuccuga gcugcucgcc ugggcagaug agcaggcccg cagcugggcu      660

cauauugagc ccauguacgu gccaccugau gguccaagcc ucgaagccug a               711


<210>  24
<211>  236
<212>  PRT
<213>  Mus musculus

<400>  24

Met Ser Glu Pro Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu 
1               5                   10                  15      


Ala Thr Gly Leu Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu 
            20                  25                  30          


Phe Ala Val His Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser 
        35                  40                  45              


Gly Ser Leu Val Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met 
    50                  55                  60                  


Cys Pro Glu Arg Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu 
65                  70                  75                  80  


Ser Ser Glu Ser Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala 
                85                  90                  95      


Val Val Arg Thr Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile 
            100                 105                 110         


Cys Leu Val Ala His Asn Gly Phe Asp Tyr Asp Phe Pro Leu Leu Cys 
        115                 120                 125             


Thr Glu Leu Gln Arg Leu Gly Ala His Leu Pro Gln Asp Thr Val Cys 
    130                 135                 140                 


Leu Asp Thr Leu Pro Ala Leu Arg Gly Leu Asp Arg Ala His Ser His 
145                 150                 155                 160 


Gly Thr Arg Ala Gln Gly Arg Lys Ser Tyr Ser Leu Ala Ser Leu Phe 
                165                 170                 175     


His Arg Tyr Phe Gln Ala Glu Pro Ser Ala Ala His Ser Ala Glu Gly 
            180                 185                 190         


Asp Val His Thr Leu Leu Leu Ile Phe Leu His Arg Ala Pro Glu Leu 
        195                 200                 205             


Leu Ala Trp Ala Asp Glu Gln Ala Arg Ser Trp Ala His Ile Glu Pro 
    210                 215                 220                 


Met Tyr Val Pro Pro Asp Gly Pro Ser Leu Glu Ala 
225                 230                 235     


<210>  25
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  25

Gly Gly Gly 
1           


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  26

Asp Gly Gly Gly Ser 
1               5   


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  27

Thr Gly Glu Lys Pro 
1               5   


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  28

Gly Gly Arg Arg 
1               


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  29

Gly Gly Gly Gly Ser 
1               5   


<210>  30
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  30

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  31
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  31

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  32

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  33

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  34
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  34

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  35
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  35

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  36
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  36

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  37

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  38

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  39

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  40
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  40

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  41
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  41

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  42
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  42

Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10                  


<210>  43
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  43

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  44
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  44

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  45
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  45

Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10              


<210>  46
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  46

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  47
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  47

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  48
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  48

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  49
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  49

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  50
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  50

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  51
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  51

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  52
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  52

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  53
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  53

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  54
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  54

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  55
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  55

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  56
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  56

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  57
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  57

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  58
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  58

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  59
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  59

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  60
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  60

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  61
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  61

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  62
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus Kozak sequence

<400>  62
gccrccatgg                                                              10


<210>  63
<211>  30
<212>  DNA
<213>  Homo sapiens

<400>  63
gctttgtgtg tgagtatgca tctccaggca                                        30


<210>  64
<211>  30
<212>  DNA
<213>  Homo sapiens

<400>  64
tgcctggaga tgcatactca cacacaaatc                                        30


<210>  65
<211>  293
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  65

Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
1               5                   10                  15      


Phe Leu Leu Arg Ile Arg Asn Asn Asn Lys Ser Ser Val Gly Tyr Ser 
            20                  25                  30          


Thr Glu Leu Gly Phe Gln Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
        35                  40                  45              


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Val Ile Ala Asn Ser 
    50                  55                  60                  


Gly Asp Asn Ala Val Ser Leu Lys Val Thr Arg Phe Glu Asp Leu Lys 
65                  70                  75                  80  


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
                85                  90                  95      


Gly Asp Tyr Met Leu Phe Lys Gln Ala Phe Cys Val Met Glu Asn Lys 
            100                 105                 110         


Glu His Leu Lys Ile Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
        115                 120                 125             


Lys Leu Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala Phe Pro Glu 
    130                 135                 140                 


Ile Ile Ser Lys Glu Arg Ser Leu Ile Asn Lys Asn Ile Pro Asn Phe 
145                 150                 155                 160 


Lys Trp Leu Ala Gly Phe Thr Ser Gly Glu Gly Cys Phe Phe Val Asn 
                165                 170                 175     


Leu Ile Lys Ser Lys Ser Lys Leu Gly Val Gln Val Gln Leu Val Phe 
            180                 185                 190         


Ser Ile Thr Gln His Ile Lys Asp Lys Asn Leu Met Asn Ser Leu Ile 
        195                 200                 205             


Thr Tyr Leu Gly Cys Gly Tyr Ile Lys Glu Lys Asn Lys Ser Glu Phe 
    210                 215                 220                 


Ser Trp Leu Asp Phe Val Val Thr Lys Phe Ser Asp Ile Asn Asp Lys 
225                 230                 235                 240 


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
                245                 250                 255     


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
            260                 265                 270         


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
        275                 280                 285             


Met Asn Lys Gly Arg 
    290             


<210>  66
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant CTLA4.B3

<400>  66

Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
1               5                   10                  15      


Phe His Leu Ser Ile Arg Asn Ser Asn Gly Tyr Arg Ser Gly Tyr Lys 
            20                  25                  30          


Thr Ser Leu Ser Phe Ser Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
        35                  40                  45              


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Thr Ile Ala Asn His 
    50                  55                  60                  


Gly Asp His Ala Val Ile Leu Thr Val Arg Arg Phe Glu Asp Leu Lys 
65                  70                  75                  80  


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
                85                  90                  95      


Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Ile Glu Asn Lys 
            100                 105                 110         


Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
        115                 120                 125             


Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala Phe Pro Glu 
    130                 135                 140                 


Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Leu 
145                 150                 155                 160 


Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Gly Val Glu 
                165                 170                 175     


Leu Val Lys Arg Ser Arg His Gly Lys Val Gly Val Arg Leu Arg Phe 
            180                 185                 190         


Ser Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile 
        195                 200                 205             


Thr Tyr Leu Gly Cys Gly Tyr Ile Asp Glu Arg Asn Ser Ser Glu Lys 
    210                 215                 220                 


Ser Trp Leu Glu Phe Arg Val Thr Lys Phe Ser Asp Ile Asn Asp Lys 
225                 230                 235                 240 


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
                245                 250                 255     


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
            260                 265                 270         


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
        275                 280                 285             


Met Asn Lys Gly Arg 
    290             


<210>  67
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant CTLA4.B3.B6

<400>  67

Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
1               5                   10                  15      


Phe His Leu Ser Ile Arg Asn Asn Asn Gly Tyr Arg Ser Gly Tyr Lys 
            20                  25                  30          


Thr Ser Leu Ser Phe Ser Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
        35                  40                  45              


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Thr Ile Ala Asn His 
    50                  55                  60                  


Gly Asp His Ala Val Ile Leu Thr Val Arg Arg Phe Glu Asp Leu Lys 
65                  70                  75                  80  


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
                85                  90                  95      


Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Ile Glu Asn Lys 
            100                 105                 110         


Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
        115                 120                 125             


Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala Phe Pro Glu 
    130                 135                 140                 


Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Leu 
145                 150                 155                 160 


Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Gly Val Glu 
                165                 170                 175     


Leu Val Lys Arg Ser Arg His Gly Lys Val Gly Val Arg Leu Arg Phe 
            180                 185                 190         


Ser Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile 
        195                 200                 205             


Thr Tyr Leu Gly Cys Gly Tyr Ile Asp Glu Arg Asn Ser Ser Glu Lys 
    210                 215                 220                 


Ser Trp Leu Glu Phe Arg Val Thr Lys Phe Ser Asp Ile Asn Asp Lys 
225                 230                 235                 240 


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
                245                 250                 255     


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
            260                 265                 270         


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
        275                 280                 285             


Met Asn Lys Gly Arg 
    290             


<210>  68
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant CTLA4.B3.B6.D5

<400>  68

Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser 
1               5                   10                  15      


Phe His Leu Ser Ile Arg Asn Asn Asn Gly Tyr Arg Ser Gly Tyr Lys 
            20                  25                  30          


Thr Ser Leu Ser Phe Ser Ile Thr Leu His Asn Lys Asp Lys Ser Ile 
        35                  40                  45              


Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Thr Ile Ala Asn His 
    50                  55                  60                  


Gly Asp His Ala Val Ile Leu Thr Val Arg Arg Phe Glu Asp Leu Lys 
65                  70                  75                  80  


Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu 
                85                  90                  95      


Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Val Ile Glu Asn Lys 
            100                 105                 110         


Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala 
        115                 120                 125             


Lys Met Asn Trp Gly Leu Thr Asp Glu Leu Lys Lys Ala Phe Pro Glu 
    130                 135                 140                 


Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Leu 
145                 150                 155                 160 


Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Gly Val Glu 
                165                 170                 175     


Leu Val Lys Arg Ser Arg His Gly Lys Val Gly Val Arg Leu Arg Phe 
            180                 185                 190         


Ser Ile Gly Gln His Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile 
        195                 200                 205             


Thr Tyr Leu Gly Cys Gly Tyr Ile Asp Glu Arg Asn Ser Ser Glu Arg 
    210                 215                 220                 


Ser Trp Leu Glu Phe Arg Val Thr Lys Phe Ser Asp Ile Asn Asp Lys 
225                 230                 235                 240 


Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys Leu Glu 
                245                 250                 255     


Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys 
            260                 265                 270         


His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn 
        275                 280                 285             


Met Asn Lys Gly Arg 
    290             


<210>  69
<211>  42
<212>  DNA
<213>  Homo sapiens

<400>  69
cctcagtggc tttgcctgga gatgcatact cacacacaaa tc                          42


<210>  70
<211>  42
<212>  DNA
<213>  Homo sapiens

<400>  70
gatttgtgtg tgagtatgca tctccaggca aagccactga gg                          42


