                         SEQUENCE LISTING

<110>  Proplex, LLC
       Chang, Frank N.
       Tuszynski, George P
        Luo, Solomon
       Luo, Solomon
       Yang, Jeff
 
<120>  SENSITIVE DETECTION OF G-PROTEIN COUPLED RECEPTOR-ASSOCIATED 
       SORTING PROTEIN 1 (GASP-1), GASP-1 MICROVESICLES, AND GASP-1 
       EXOSOMES

<130>  PRX-100WO

<150>  US 62/608,962
<151>  2017-12-21

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1395
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Thr Gly Ala Glu Ile Glu Ser Gly Ala Gln Val Lys Pro Glu Lys 
1               5                   10                  15      


Lys Pro Gly Glu Glu Val Val Gly Gly Ala Glu Ile Glu Asn Asp Val 
            20                  25                  30          


Pro Leu Val Val Arg Pro Lys Val Arg Thr Gln Ala Gln Ile Met Pro 
        35                  40                  45              


Gly Ala Arg Pro Lys Asn Lys Ser Lys Val Met Pro Gly Ala Ser Thr 
    50                  55                  60                  


Lys Val Glu Thr Ser Ala Val Gly Gly Ala Arg Pro Lys Ser Lys Ala 
65                  70                  75                  80  


Lys Ala Ile Pro Val Ser Arg Phe Lys Glu Glu Ala Gln Met Trp Ala 
                85                  90                  95      


Gln Pro Arg Phe Gly Ala Glu Arg Leu Ser Lys Thr Glu Arg Asn Ser 
            100                 105                 110         


Gln Thr Asn Ile Ile Ala Ser Pro Leu Val Ser Thr Asp Ser Val Leu 
        115                 120                 125             


Val Ala Lys Thr Lys Tyr Leu Ser Glu Asp Arg Glu Leu Val Asn Thr 
    130                 135                 140                 


Asp Thr Glu Ser Phe Pro Arg Arg Lys Ala His Tyr Gln Ala Gly Phe 
145                 150                 155                 160 


Gln Pro Ser Phe Arg Ser Lys Glu Glu Thr Asn Met Gly Ser Trp Cys 
                165                 170                 175     


Cys Pro Arg Pro Thr Ser Lys Gln Glu Ala Ser Pro Asn Ser Asp Phe 
            180                 185                 190         


Lys Trp Val Asp Lys Ser Val Ser Ser Leu Phe Trp Ser Gly Asp Glu 
        195                 200                 205             


Val Thr Ala Lys Phe His Pro Gly Asn Arg Val Lys Asp Ser Asn Arg 
    210                 215                 220                 


Ser Met His Met Ala Asn Gln Glu Ala Asn Thr Met Ser Arg Ser Gln 
225                 230                 235                 240 


Thr Asn Gln Glu Leu Tyr Ile Ala Ser Ser Ser Gly Ser Glu Asp Glu 
                245                 250                 255     


Ser Val Lys Thr Pro Trp Phe Trp Ala Arg Asp Lys Thr Asn Thr Trp 
            260                 265                 270         


Ser Gly Pro Arg Glu Asp Pro Asn Ser Arg Ser Arg Phe Arg Ser Lys 
        275                 280                 285             


Lys Glu Val Tyr Val Glu Ser Ser Ser Gly Ser Glu His Glu Asp His 
    290                 295                 300                 


Leu Glu Ser Trp Phe Gly Ala Gly Lys Glu Ala Lys Phe Arg Ser Lys 
305                 310                 315                 320 


Met Arg Ala Gly Lys Glu Ala Asn Asn Arg Ala Arg His Arg Ala Lys 
                325                 330                 335     


Arg Glu Ala Cys Ile Asp Phe Met Pro Gly Ser Ile Asp Val Ile Lys 
            340                 345                 350         


Lys Glu Ser Cys Phe Trp Pro Glu Glu Asn Ala Asn Thr Phe Ser Arg 
        355                 360                 365             


Pro Met Ile Lys Lys Glu Ala Arg Ala Arg Ala Met Thr Lys Glu Glu 
    370                 375                 380                 


Ala Lys Thr Lys Ala Arg Ala Arg Ala Lys Gln Glu Ala Arg Ser Glu 
385                 390                 395                 400 


Glu Glu Ala Leu Ile Gly Thr Trp Phe Trp Ala Thr Asp Glu Ser Ser 
                405                 410                 415     


Met Ala Asp Glu Ala Ser Ile Glu Ser Ser Leu Gln Val Glu Asp Glu 
            420                 425                 430         


Ser Ile Ile Gly Ser Trp Phe Trp Thr Glu Glu Glu Ala Ser Met Gly 
        435                 440                 445             


Thr Gly Ala Ser Ser Lys Ser Arg Pro Arg Thr Asp Gly Glu Arg Ile 
    450                 455                 460                 


Gly Asp Ser Leu Phe Gly Ala Arg Glu Lys Thr Ser Met Lys Thr Gly 
465                 470                 475                 480 


Ala Glu Ala Thr Ser Glu Ser Ile Leu Ala Ala Asp Asp Glu Gln Val 
                485                 490                 495     


Ile Ile Gly Ser Trp Phe Trp Ala Gly Glu Glu Val Asn Gln Glu Ala 
            500                 505                 510         


Glu Glu Glu Thr Ile Phe Gly Ser Trp Phe Trp Val Ile Asp Ala Ala 
        515                 520                 525             


Ser Val Glu Ser Gly Val Gly Val Ser Cys Glu Ser Arg Thr Arg Ser 
    530                 535                 540                 


Glu Glu Glu Glu Val Ile Gly Pro Trp Phe Trp Ser Gly Glu Gln Val 
545                 550                 555                 560 


Asp Ile Glu Ala Gly Ile Gly Glu Glu Ala Arg Pro Gly Ala Glu Glu 
                565                 570                 575     


Glu Thr Ile Phe Gly Ser Trp Phe Trp Ala Glu Asn Gln Thr Tyr Met 
            580                 585                 590         


Asp Cys Arg Ala Glu Thr Ser Cys Asp Thr Met Gln Gly Ala Glu Glu 
        595                 600                 605             


Glu Glu Pro Ile Ile Gly Ser Trp Phe Trp Thr Arg Val Glu Ala Cys 
    610                 615                 620                 


Val Glu Gly Asp Val Asn Ser Lys Ser Ser Leu Glu Asp Lys Glu Glu 
625                 630                 635                 640 


Ala Met Ile Pro Cys Phe Gly Ala Lys Glu Glu Val Ser Met Lys His 
                645                 650                 655     


Gly Thr Gly Val Arg Cys Arg Phe Met Ala Gly Ala Glu Glu Thr Asn 
            660                 665                 670         


Asn Lys Ser Cys Phe Trp Ala Glu Lys Glu Pro Cys Met Tyr Pro Ala 
        675                 680                 685             


Gly Gly Gly Ser Trp Lys Ser Arg Pro Glu Glu Glu Glu Asp Ile Val 
    690                 695                 700                 


Asn Ser Trp Phe Trp Ser Arg Lys Tyr Thr Lys Pro Glu Ala Ile Ile 
705                 710                 715                 720 


Gly Ser Trp Leu Trp Ala Thr Glu Glu Ser Asn Ile Asp Gly Thr Gly 
                725                 730                 735     


Glu Lys Ala Lys Leu Leu Thr Glu Glu Glu Thr Ile Ile Asn Ser Trp 
            740                 745                 750         


Phe Trp Lys Glu Asp Glu Ala Ile Ser Glu Ala Thr Asp Arg Glu Glu 
        755                 760                 765             


Ser Arg Pro Glu Ala Glu Glu Gly Asp Ile Ile Gly Ser Trp Phe Trp 
    770                 775                 780                 


Ala Gly Glu Glu Asp Arg Leu Glu Pro Ala Ala Glu Thr Arg Glu Glu 
785                 790                 795                 800 


Asp Arg Leu Ala Ala Glu Lys Glu Gly Ile Val Gly Ser Trp Phe Gly 
                805                 810                 815     


Ala Arg Glu Glu Thr Ile Arg Arg Glu Ala Gly Ser Cys Ser Lys Ser 
            820                 825                 830         


Ser Pro Lys Ala Glu Glu Glu Glu Val Ile Ile Gly Ser Trp Phe Trp 
        835                 840                 845             


Glu Glu Glu Ala Ser Pro Glu Ala Val Ala Gly Val Gly Phe Glu Ser 
    850                 855                 860                 


Lys Pro Gly Thr Glu Glu Glu Glu Ile Thr Val Gly Ser Trp Phe Trp 
865                 870                 875                 880 


Pro Glu Glu Glu Ala Ser Ile Gln Ala Gly Ser Gln Ala Val Glu Glu 
                885                 890                 895     


Met Glu Ser Glu Thr Glu Glu Glu Thr Ile Phe Gly Ser Trp Phe Trp 
            900                 905                 910         


Asp Gly Lys Glu Val Ser Glu Glu Ala Gly Pro Cys Cys Val Ser Lys 
        915                 920                 925             


Pro Glu Asp Asp Glu Glu Met Ile Val Glu Ser Trp Phe Trp Ser Arg 
    930                 935                 940                 


Asp Lys Ala Ile Lys Glu Thr Gly Thr Val Ala Thr Cys Glu Ser Lys 
945                 950                 955                 960 


Pro Glu Asn Glu Glu Gly Ala Ile Val Gly Ser Trp Phe Glu Ala Glu 
                965                 970                 975     


Asp Glu Val Asp Asn Arg Thr Asp Asn Gly Ser Asn Cys Gly Ser Arg 
            980                 985                 990         


Thr Leu Ala Asp Glu Asp Glu Ala  Ile Val Gly Ser Trp  Phe Trp Ala 
        995                 1000                 1005             


Gly Asp  Glu Ala His Phe Glu  Ser Asn Pro Ser Pro  Val Phe Arg 
    1010                 1015                 1020             


Ala Ile  Cys Arg Ser Thr Cys  Ser Val Glu Gln Glu  Pro Asp Pro 
    1025                 1030                 1035             


Ser Arg  Arg Pro Gln Ser Trp  Glu Glu Val Thr Val  Gln Phe Lys 
    1040                 1045                 1050             


Pro Gly  Pro Trp Gly Arg Val  Gly Phe Pro Ser Ile  Ser Pro Phe 
    1055                 1060                 1065             


Arg Phe  Pro Lys Glu Ala Ala  Ser Leu Phe Cys Glu  Met Phe Gly 
    1070                 1075                 1080             


Gly Lys  Pro Arg Asn Met Val  Leu Ser Pro Glu Gly  Glu Asp Gln 
    1085                 1090                 1095             


Glu Ser  Leu Leu Gln Pro Asp  Gln Pro Ser Pro Glu  Phe Pro Phe 
    1100                 1105                 1110             


Gln Tyr  Asp Pro Ser Tyr Arg  Ser Val Gln Glu Ile  Arg Glu His 
    1115                 1120                 1125             


Leu Arg  Ala Lys Glu Ser Thr  Glu Pro Glu Ser Ser  Ser Cys Asn 
    1130                 1135                 1140             


Cys Ile  Gln Cys Glu Leu Lys  Ile Gly Ser Glu Glu  Phe Glu Glu 
    1145                 1150                 1155             


Leu Leu  Leu Leu Met Glu Lys  Ile Arg Asp Pro Phe  Ile His Glu 
    1160                 1165                 1170             


Ile Ser  Lys Ile Ala Met Gly  Met Arg Ser Ala Ser  Gln Phe Thr 
    1175                 1180                 1185             


Arg Asp  Phe Ile Arg Asp Ser  Gly Val Val Ser Leu  Ile Glu Thr 
    1190                 1195                 1200             


Leu Leu  Asn Tyr Pro Ser Ser  Arg Val Arg Thr Ser  Phe Leu Glu 
    1205                 1210                 1215             


Asn Met  Ile Arg Met Ala Pro  Pro Tyr Pro Asn Leu  Asn Ile Ile 
    1220                 1225                 1230             


Gln Thr  Tyr Ile Cys Lys Val  Cys Glu Glu Thr Leu  Ala Tyr Ser 
    1235                 1240                 1245             


Val Asp  Ser Pro Glu Gln Leu  Ser Gly Ile Arg Met  Ile Arg His 
    1250                 1255                 1260             


Leu Thr  Thr Thr Thr Asp Tyr  His Thr Leu Val Ala  Asn Tyr Met 
    1265                 1270                 1275             


Ser Gly  Phe Leu Ser Leu Leu  Ala Thr Gly Asn Ala  Lys Thr Arg 
    1280                 1285                 1290             


Phe His  Val Leu Lys Met Leu  Leu Asn Leu Ser Glu  Asn Leu Phe 
    1295                 1300                 1305             


Met Thr  Lys Glu Leu Leu Ser  Ala Glu Ala Val Ser  Glu Phe Ile 
    1310                 1315                 1320             


Gly Leu  Phe Asn Arg Glu Glu  Thr Asn Asp Asn Ile  Gln Ile Val 
    1325                 1330                 1335             


Leu Ala  Ile Phe Glu Asn Ile  Gly Asn Asn Ile Lys  Lys Glu Thr 
    1340                 1345                 1350             


Val Phe  Ser Asp Asp Asp Phe  Asn Ile Glu Pro Leu  Ile Ser Ala 
    1355                 1360                 1365             


Phe His  Lys Val Glu Lys Phe  Ala Lys Glu Leu Gln  Gly Lys Thr 
    1370                 1375                 1380             


Asp Asn  Gln Asn Asp Pro Glu  Gly Asp Gln Glu Asn  
    1385                 1390                 1395 


<210>  2
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  2

Glu Glu Ala Ser Pro Glu Ala Val Ala Gly Val Gly Phe Glu Ser Lys 
1               5                   10                  15      


<210>  3
<211>  13
<212>  PRT
<213>  Homo sapiens

<400>  3

Cys Ser Lys Ser Ser Pro Lys Ala Glu Glu Glu Glu Val 
1               5                   10              


<210>  4
<211>  14
<212>  PRT
<213>  Homo sapiens

<400>  4

Phe Trp Asp Gly Lys Glu Val Ser Glu Glu Ala Gly Pro Cys 
1               5                   10                  


