               SEQUENCE LISTING

<110> Glycom A/S

<120> NUCLEIC ACID CONSTRUCT

<130> 122WO00

<150> DK201800231
<151> 2018-05-24
<150> DK201800173
<151> 2018-04-18
<150> DK201700737
<151> 2017-12-21

<160> 117

<170> BiSSAP 1.3.6

<210> 1
<211> 284
<212> DNA
<213> Artificial Sequence


<220> 
<223> 284-nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located 16 bp upstream the glpF
      translation initiation codon

<400> 1
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taac                     284


<210> 2
<211> 166
<212> DNA
<213> Artificial Sequence


<220> 
<223> 166-nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located 16 bp upstream of the glpA
      translation initiation codon

<400> 2
gaaaacattc ataaattaaa tgtgaattgc cgcacacatt attaaataag atttacaaaa     60

tgttcaaaat gacgcatgaa atcacgtttc actttcgaat tatgagcgaa tatgcgcgaa    120

atcaaacaat tcatgttttt actatggcta aatggtaaaa aacgaa                   166


<210> 3
<211> 174
<212> DNA
<213> Artificial Sequence


<220> 
<223> 174 bp DNA fragment derived from genomic DNA of E. coli (ref. seq
      ID U00096.3). located 16 bp upstream of the glpD translation
      initiation codon

<400> 3
tgcgtctctc tttctttaca aacaagtggg caaatttacc gcacagttta cgtcgaagcg     60

gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa tgttacctaa    120

agcgcgattc tttgctaata tgttcgataa cgaacattta tgagctttaa cgaa          174


<210> 4
<211> 229
<212> DNA
<213> Artificial Sequence


<220> 
<223> 229 bp DNA fragment derived from genomic DNA of E. coli (ref. seq
      ID U00096.3). Located 60 bp upstream of the glpT translation
      initiation codon

<400> 4
ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg     60

aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt    120

gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac    180

ggcaggttct ctcactgaat caggctgtta atcataaata agaccacgg                229


<210> 5
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16-nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located directly upstream the
      translational initiation codon of glpF

<400> 5
tcttcaggat ccgatt                                                    16


<210> 6
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16-nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located directly upstream the
      translational initiation codon of glpA

<400> 6
cttcagaggg ataaca                                                    16


<210> 7
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16-nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located upstream the translational
      initiation codon of glpD

<400> 7
agtgaatgag ggcagc                                                    16


<210> 8
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16 -nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located directly upstream the
      translational initiation codon of glpT

<400> 8
gccacggagg ctatca                                                    16


<210> 9
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16-nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3). located upstream of lacZ

<400> 9
cacacaggaa acagct                                                    16


<210> 10
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 9 ) (CAC -> AGG )

<400> 10
caaggaggaa acagct                                                    16


<210> 11
<211> 107
<212> DNA
<213> Artificial Sequence


<220> 
<223> 107-nucleotide DNA fragment located upstream of lacZ derived from
      genomic DNA of E. coli (ref. seq ID U00096.3); lac operon
      promoter element

<400> 11
tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat     60

gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagct                  107


<210> 12
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300 -nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO: 10

<400> 12
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300


<210> 13
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO: 38

<400> 13
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaaatt cgaaacagct    300


<210> 14
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO: 39

<400> 14
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagcg caaaacagct    300


<210> 15
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300 -nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO:40

<400> 15
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagaa caaaacagct    300


<210> 16
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO:41

<400> 16
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaacta ggaaacagct    300


<210> 17
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO:42

<400> 17
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaaccg agaaacagct    300


<210> 18
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO:43

<400> 18
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagag ctaaacagct    300


<210> 19
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO:44

<400> 19
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagag caaaacagct    300


<210> 20
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO:45

<400> 20
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagag aaaaacagct    300


<210> 21
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO:46

<400> 21
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaaagg aaaaacagct    300


<210> 22
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA fragment comprising SEQ ID NO: 1 and SEQ ID
      NO: 47

<400> 22
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaactg agaaacagct    300


<210> 23
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO:12 comprising a modification of the -10
      region (See Figure 7)

<400> 23
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgattt aattacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300


<210> 24
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO:12 comprising a modification of the -10
      region

<400> 24
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgatca gaatacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300


<210> 25
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO:12 comprising a modification of the -10
      region

<400> 25
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgatat tcctacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300


<210> 26
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO:12 comprising a modification of the -10
      region

<400> 26
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa tgatacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300


<210> 27
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO:12 comprising a modification of the -10
      region

<400> 27
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgatga agctacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300


<210> 28
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO:12 comprising a modification of the -10
      region

<400> 28
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgatca gtatacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    300


<210> 29
<211> 285
<212> DNA
<213> Artificial Sequence


<220> 
<223> 285-nucleotide DNA fragment of SEQ ID NO: 12

<400> 29
gattacggtt tgccacactt ttcatccttc tcctggtgac ataatccaca tcaatcgaaa     60

atgttaataa atttgttgcg cgaatgatct aacaaacatg catcatgtac aatcagatgg    120

aataaatggc gcgataacgc tcattttatg acgaggcaca cacattttaa gttcgatatt    180

tctcgttttt gctcgttaac gataagttta cagcatgcct acaagcatcg tggaggtccg    240

tgactttcac gcatacaaca aacattaacc aaggaggaaa cagct                    285


<210> 30
<211> 160
<212> DNA
<213> Artificial Sequence


<220> 
<223> 160-nucleotide DNA fragment of SEQ ID NO: 12

<400> 30
atggcgcgat aacgctcatt ttatgacgag gcacacacat tttaagttcg atatttctcg     60

tttttgctcg ttaacgataa gtttacagca tgcctacaag catcgtggag gtccgtgact    120

ttcacgcata caacaaacat taaccaagga ggaaacagct                          160


<210> 31
<211> 135
<212> DNA
<213> Artificial Sequence


<220> 
<223> 135-nucleotide DNA fragment of SEQ ID NO: 12

<400> 31
acgaggcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta     60

cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaacc    120

aaggaggaaa cagct                                                     135


<210> 32
<211> 120
<212> DNA
<213> Artificial Sequence


<220> 
<223> 120-nucleotide DNA fragment of SEQ ID NO: 12

<400> 32
tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag     60

catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga ggaaacagct    120


<210> 33
<211> 182
<212> DNA
<213> Artificial Sequence


<220> 
<223> 182-nucleotide DNA fragment comprising SEQ ID NO: 2 and SEQ ID
      NO: 10

<400> 33
gaaaacattc ataaattaaa tgtgaattgc cgcacacatt attaaataag atttacaaaa     60

tgttcaaaat gacgcatgaa atcacgtttc actttcgaat tatgagcgaa tatgcgcgaa    120

atcaaacaat tcatgttttt actatggcta aatggtaaaa aacgaacaag gaggaaacag    180

ct                                                                   182


<210> 34
<211> 190
<212> DNA
<213> Artificial Sequence


<220> 
<223> 190-nucleotide DNA fragment comprising SEQ ID NO: 3 and SEQ ID
      NO: 10

<400> 34
tgcgtctctc tttctttaca aacaagtggg caaatttacc gcacagttta cgtcgaagcg     60

gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa tgttacctaa    120

agcgcgattc tttgctaata tgttcgataa cgaacattta tgagctttaa cgaacaagga    180

ggaaacagct                                                           190


<210> 35
<211> 245
<212> DNA
<213> Artificial Sequence


<220> 
<223> 245-nucleotide DNA fragment comprising SEQ ID NO: 4 and SEQ ID
      NO: 10

<400> 35
ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg     60

aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt    120

gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac    180

ggcaggttct ctcactgaat caggctgtta atcataaata agaccacggc aaggaggaaa    240

cagct                                                                245


<210> 36
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> 54-nucleotide DNA fragment of the 5’UTR- glpF located downstream
      of the transcription initiation site and 16 nucleotides upstream
      the translation initiation codon

<400> 36
tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat taac           54


<210> 37
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> 70-nucleotide synthetic non-coding DNA sequence (ii) comprising
      SEQ ID NO: 36 and SEQ ID NO: 10

<400> 37
tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat taaccaagga     60

ggaaacagct                                                            70


<210> 38
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 38
caaattcgaa acagct                                                    16


<210> 39
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 39
caagcgcaaa acagct                                                    16


<210> 40
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 40
caagaacaaa acagct                                                    16


<210> 41
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 41
caactaggaa acagct                                                    16


<210> 42
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 42
caaccgagaa acagct                                                    16


<210> 43
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 43
caagagctaa acagct                                                    16


<210> 44
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 44
caagagcaaa acagct                                                    16


<210> 45
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 45
caagagaaaa acagct                                                    16


<210> 46
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 46
caaaggaaaa acagct                                                    16


<210> 47
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Variant of SEQ ID NO: 10

<400> 47
caactgagaa acagct                                                    16


<210> 48
<211> 119
<212> DNA
<213> Artificial Sequence


<220> 
<223> 119-nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located upstream of the glpA transcription
      initiation start; glpA promoter element

<400> 48
gaaaacattc ataaattaaa tgtgaattgc cgcacacatt attaaataag atttacaaaa     60

tgttcaaaat gacgcatgaa atcacgtttc actttcgaat tatgagcgaa tatgcgcga     119


<210> 49
<211> 169
<212> DNA
<213> Artificial Sequence


<220> 
<223> 169-nucleotide DNA fragment derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located upstream of the glpT transcription
      initiation start; glpT promoter element

<400> 49
ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg     60

aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt    120

gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggca                169


<210> 50
<211> 189
<212> DNA
<213> Artificial Sequence


<220> 
<223> 189-nucleotide DNA fragment obtained by combining SEQ ID NO: 49
      with SEQ ID NO 37

<400> 50
gaaaacattc ataaattaaa tgtgaattgc cgcacacatt attaaataag atttacaaaa     60

tgttcaaaat gacgcatgaa atcacgtttc actttcgaat tatgagcgaa tatgcgcgat    120

gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt aaccaaggag    180

gaaacagct                                                            189


<210> 51
<211> 239
<212> DNA
<213> Artificial Sequence


<220> 
<223> 239-nucleotide DNA fragment obtained by combining SEQ ID NO: 50
      with synthetic non-coding DNA sequence (ii) (SEQ ID NO 37)

<400> 51
ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg     60

aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt    120

gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcat gcctacaagc    180

atcgtggagg tccgtgactt tcacgcatac aacaaacatt aaccaaggag gaaacagct     239


<210> 52
<211> 239
<212> DNA
<213> Artificial Sequence


<220> 
<223> 239-nucleotide DNA fragment SEQ ID NO: 50 where the 16 bp located
      upstream of the translation initiation site is identical to SEQ
      ID NO 41

<400> 52
ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg     60

aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt    120

gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcat gcctacaagc    180

atcgtggagg tccgtgactt tcacgcatac aacaaacatt aaccaactag gaaacagct     239


<210> 53
<211> 239
<212> DNA
<213> Artificial Sequence


<220> 
<223> 239-nucleotide DNA fragment SEQ ID NO: 50 where the 16 bp located
      upstream of the translation initiation site is identical to SEQ
      ID NO 46

<400> 53
ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg     60

aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt    120

gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcat gcctacaagc    180

atcgtggagg tccgtgactt tcacgcatac aacaaacatt aaccaaagga aaaacagct     239


<210> 54
<211> 230
<212> DNA
<213> Artificial Sequence


<220> 
<223> 230-nucleotide DNA sequence derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located upstream the initiation of
      transcription of glpF; glpF promoter element

<400> 54
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca               230


<210> 55
<211> 245
<212> DNA
<213> Artificial Sequence


<220> 
<223> 245-nucleotide nucleotide DNA sequence derived from genomic DNA
      of E. coli (ref. seq ID U00096.3) located upstream the initiation
      of translation of glpT

<400> 55
ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg     60

aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt    120

gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac    180

ggcaggttct ctcactgaat caggctgtta atcataaata agaccacggg ccacggaggc    240

tatca                                                                245


<210> 56
<211> 182
<212> DNA
<213> Artificial Sequence


<220> 
<223> 182-nucleotide DNA sequence derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located upstream the initiation of
      translation of glpA

<400> 56
gaaaacattc ataaattaaa tgtgaattgc cgcacacatt attaaataag atttacaaaa     60

tgttcaaaat gacgcatgaa atcacgtttc actttcgaat tatgagcgaa tatgcgcgaa    120

atcaaacaat tcatgttttt actatggcta aatggtaaaa aacgaacttc agagggataa    180

ca                                                                   182


<210> 57
<211> 300
<212> DNA
<213> Artificial Sequence


<220> 
<223> 300-nucleotide DNA sequence derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located upstream the initiation of
      translation of glpF

<400> 57
gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat     60

ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca    120

tgtacaatca gatggaataa atggcgcgat aacgctcatt ttatgacgag gcacacacat    180

tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag    240

catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca ggatccgatt    300


<210> 58
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> combined DNA/RNA artificial sequence - primer

<400> 58
cccagcgaga cctgaccgca gaac                                           24


<210> 59
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 59
ccccagtcca tcagcgtgac tacc                                           24


<210> 60
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 60
atgcgcaaau cggatctcaa ggaaatcgca atgg                                 34


<210> 61
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 61
agctgttucc tccttgctca ttgtcatagt gcggcagg                             38


<210> 62
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 62
atgcgcaaau gctgaatccg aacaccagcg tc                                   32


<210> 63
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 63
agctgttucc tccttggcag gacttcatta ttaagacgg                            39


<210> 64
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 64
atgcgcaaau tactcactac tgaaacaata ttgcc                                35


<210> 65
<211> 44
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 65
agctgttucc tccttgtaat cctatttaaa tttttgctga atag                      44


<210> 66
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 66
atgcgcaaau ccgaaaattc ctggcgagca g                                   31


<210> 67
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 67
agctgttucc tccttggatg ttatgtccca gtaattaac                            39


<210> 68
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 68
atgcgcaaau gaagtaatct ttcttcacct gcgttc                               36


<210> 69
<211> 44
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 69
agctgttucc tccttggtta ttttttattg tgaattaaga tagg                      44


<210> 70
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 70
atgcgcaaau cagttcttct gccgaaggtt                                     30


<210> 71
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 71
agctgttucc tccttgttgt tagtgaataa aaggttgcc                            39


<210> 72
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 72
atgcgcaaau gaaaacattc ataaattaaa tgtg                                 34


<210> 73
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 73
agctgttucc tccttgttcg ttttttacca tttagccata g                         41


<210> 74
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 74
tgcgcaaaug cgtctctctt tctttacaaa c                                   31


<210> 75
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 75
agctgttucc tccttgttcg ttaaagctca taaatgttcg                           40


<210> 76
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 76
atgcgcaaau gcggcacgcc ttgcagatta cg                                   32


<210> 77
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 77
agctgttucc tccttggtta atgtttgttg tatgcg                               36


<210> 78
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 78
atgcgcaaau ccatttagcc atagtaaaaa catg                                 34


<210> 79
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 79
gctgttucct ccttgccgtg gtcttattta tgattaac                             38


<210> 80
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 80
atgcgcaaau gcgcgggttc cgtgcgtggg                                     30


<210> 81
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 81
agctgttucc tccttgattt attactgaaa gggccgc                              37


<210> 82
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 82
atgcgcaaau gtgatcacaa attttaaaca g                                   31


<210> 83
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 83
agctgttucc tccttgacag tgttaccgtt acgatac                              37


<210> 84
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 84
atgcgcaaau tgtgagttag ctcactcatt ag                                   32


<210> 85
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 85
agctgttucc tccttgaaat tgttatccgc tcacaa                               36


<210> 86
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 86
atgcgcaaau gaatgctctc aggtgaggg                                      29


<210> 87
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 87
agctgttucc tccttgtttc gcgctccgaa ataatc                               36


<210> 88
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 88
atgcgcaaau ccgaaatcgc tgaaggttac gtac                                 34


<210> 89
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 89
agctgttucc tccttgaatg tgataacggt aacaagttta g                         41


<210> 90
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 90
atgcgcaaau ggctgtgttg aaaggtgttg                                     30


<210> 91
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 91
agctgttucc tccttgagta tgggtgcttt ttttacg                              37


<210> 92
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 92
atgcgcaaau gaattgcaac agtaatgcca g                                   31


<210> 93
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 93
agctgttucc tccttgatag gtttagtgtt gtggaac                              37


<210> 94
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 94
atgcgcaaau gaaaacattc ataaattaaa tgtg                                 34


<210> 95
<211> 93
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 95
agctgttucc tccttggtta atgtttgttg tatgcgtgaa agtcacggac ctccacgatg     60

cttgtaggca tcgcgcatat tcgctcataa ttc                                  93


<210> 96
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 96
atgcgcaaau ccatttagcc atagtaaaaa catg                                 34


<210> 97
<211> 90
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 97
agctgttucc tccttggtta atgtttgttg tatgcgtgaa agtcacggac ctccacgatg     60

cttgtaggca tgccgcgatg ttaagaaaac                                      90


<210> 98
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 98
aaacagcuat gtgcaatagc gataacacc                                      29


<210> 99
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 99
agggttaaut gcgcgttagg cccagaacag aacatc                               36


<210> 100
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 100
attaacccuc caggcatcaa ataaaacgaa aggc                                 34


<210> 101
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 101
atttgcgcau caccaatcaa attcacgcgg cc                                   32


<210> 102
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 102
atgcgcaaau gcggcacgcc ttgcagatta cg                                   32


<210> 103
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Combined DNA/RNA artificial sequence - primer

<400> 103
agctgttucc tccttggtta atgtttgttg tatgcg                               36


<210> 104
<211> 190
<212> DNA
<213> Artificial Sequence


<220> 
<223> 190-nucleotide DNA sequence derived from genomic DNA of E. coli
      (ref. seq ID U00096.3) located upstream the initiation of
      translation of glpD

<400> 104
tgcgtctctc tttctttaca aacaagtggg caaatttacc gcacagttta cgtcgaagcg     60

gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa tgttacctaa    120

agcgcgattc tttgctaata tgttcgataa cgaacattta tgagctttaa cgaaagtgaa    180

tgagggcagc                                                           190


<210> 105
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - Combined DNA/RNA sequence

<400> 105
aaacagcuat gatctctgtc tacatcatca gtctg                                35


<210> 106
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 106
agggttaaut gcgcgttaga cttctttcgg ggttttca                             38


<210> 107
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 107
aaacagcuat ggcgttcaaa gtggtccaaa tc                                   32


<210> 108
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 108
agggttaaut gcgcgttagc ccagcgcgtt atatttctg                            39


<210> 109
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 109
aaacagcuat gcaaccgctg gtctccgtgc                                     30


<210> 110
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 110
agggttaaut gcgcgttaac ggtttttcag caggcgg                              37


<210> 111
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 111
aaacagcuat gtcaaaagtc gctctcatca ccgg                                 34


<210> 112
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 112
agggttaaut gcgcgttact cgttcagcaa cgtcagc                              37


<210> 113
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 113
aaacagcuat gcgtgtcttc gccatttctc                                     30


<210> 114
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 114
agggttaaut gcgcgttaga cgaattgcca gtatttcagg                           40


<210> 115
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 115
aaacagcuat ggaacgtaac gccgtgagcc tgc                                  33


<210> 116
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 116
agggttaaut gcggcttagt ttttatcgtc aaaggtcag                            39


<210> 117
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer - combined DNA/RNA sequence

<400> 117
agctgttucc tccttaggta cccagctttt gttccc                               36


