                         SEQUENCE LISTING

<110>  University of Pretoria
 
<120>  TRANSGENIC PLANTS WITH ENHANCED RESISTANCE TO ABIOTIC STRESS 
       CONDITIONS

<130>  PA168341/PCT

<140>  GB 1721351.3
<141>  2017-12-19

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  497
<212>  DNA
<213>  Unknown

<220>
<223>  WHy gene metagenomic library isolate nucleotide sequence

<400>  1
atgagctatt tagcaactat aaaaaaaaca tcggcatatt tgatttttat cacattgtta       60

agtggtgttg gtggctgtgc atcgtctggt agcagcggct ttaaagaccc gacgtccagc      120

tcgttgatgt tgaactgata cacgctaagt tgcttgagca acaatttatg ctgcactttc      180

gtgtcgataa ccctaattca aaaagtttgc caatgcgcgg tatggactac cgtattctgc      240

taaataacac acctttggcc acaggtagca atagccaatg gctgacagtt cctgcacacg      300

actacgccta ctttaaaata cccgtgcata ccaatttatg gcggcatatg aaggttgttc      360

tgcgcatgct agaaaaccct gaccagccga ttcattacgc gctgcatgca gaggtaaaaa      420

ctggccttat gttcagcaaa aaaatcaata tccttcgtca cggtgatatc attcccggcg      480

actatattcg cgagtaa                                                     497


<210>  2
<211>  165
<212>  PRT
<213>  Unknown

<220>
<223>  WHy gene metagenomic library isolate amino acid sequence

<400>  2

Met Ser Tyr Leu Ala Thr Ile Lys Lys Thr Ser Ala Tyr Leu Ile Phe 
1               5                   10                  15      


Ile Thr Leu Leu Ser Gly Val Gly Gly Cys Ala Ser Ser Gly Ser Ser 
            20                  25                  30          


Gly Phe Lys Asp Pro Asp Val Gln Leu Val Asp Val Glu Leu Ile His 
        35                  40                  45              


Ala Lys Leu Leu Glu Gln Gln Phe Met Leu His Phe Arg Val Asp Asn 
    50                  55                  60                  


Pro Asn Ser Lys Ser Leu Pro Met Arg Gly Met Asp Tyr Arg Ile Leu 
65                  70                  75                  80  


Leu Asn Asn Thr Pro Leu Ala Thr Gly Ser Asn Ser Gln Trp Leu Thr 
                85                  90                  95      


Val Pro Ala His Asp Tyr Ala Tyr Phe Lys Ile Pro Val His Thr Asn 
            100                 105                 110         


Leu Trp Arg His Met Lys Val Val Leu Arg Met Leu Glu Asn Pro Asp 
        115                 120                 125             


Gln Pro Ile His Tyr Ala Leu His Ala Glu Val Lys Thr Gly Leu Met 
    130                 135                 140                 


Phe Ser Lys Lys Ile Asn Ile Leu Arg His Gly Asp Ile Ile Pro Gly 
145                 150                 155                 160 


Asp Tyr Ile Arg Glu 
                165 


<210>  3
<211>  516
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Arabidopsis codon-optimised WHy nucleotide sequence

<400>  3
atgtcatact tggctactat caaaaagacc tctgcttacc tcatattcat aaccttgctc       60

tcaggagtgg gtggttgtgc ttcatcagga tcttcaggtt ttaaggatcc tgatgttcaa      120

ctcgttgatg tggaattaat ccatgctaaa cttttggagc aacagtttat gcttcacttc      180

agagtggata atcctaattc taagtctctt ccaatgaggg gtatggatta caggatactc      240

ctcaataaca ctccactcgc aacaggttca aatagtcaat ggttaaccgt tcctgctcat      300

gattatgcat actttaagat accagtgcat actaatctct ggagacacat gaaagttgtg      360

cttaggatgt tggaaaaccc tgatcagcca attcattacg ctcttcacgc agaggttaaa      420

acaggattga tgttctctaa gaaaattaac atcttgagac acggagatat tataccagga      480

gattacatca gggagcacca ccatcatcac cattga                                516


<210>  4
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Histidine tag

<400>  4

His His His His His His 
1               5       


<210>  5
<211>  447
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Arabidopsis codon-optimised truncated WHy nucleotide sequence

<400>  5
atgggatgtg cttcatcagg atcttcaggt tttaaggatc ctgatgttca actcgttgat       60

gtggaattaa tccatgctaa acttttggag caacagttta tgcttcactt cagagtggat      120

aatcctaatt ctaagtctct tccaatgagg ggtatggatt acaggatact cctcaataac      180

actccactcg caacaggttc aaatagtcaa tggttaaccg ttcctgctca tgattatgca      240

tactttaaga taccagtgca tactaatctc tggagacaca tgaaagttgt gcttaggatg      300

ttggaaaacc ctgatcagcc aattcattac gctcttcacg cagaggttaa aacaggattg      360

atgttctcta agaaaattaa catcttgaga cacggagata ttataccagg agattacatc      420

agggagcacc accatcatca ccattga                                          447


<210>  6
<211>  148
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Truncated WHy amino acid sequence

<400>  6

Met Gly Cys Ala Ser Ser Gly Ser Ser Gly Phe Lys Asp Pro Asp Val 
1               5                   10                  15      


Gln Leu Val Asp Val Glu Leu Ile His Ala Lys Leu Leu Glu Gln Gln 
            20                  25                  30          


Phe Met Leu His Phe Arg Val Asp Asn Pro Asn Ser Lys Ser Leu Pro 
        35                  40                  45              


Met Arg Gly Met Asp Tyr Arg Ile Leu Leu Asn Asn Thr Pro Leu Ala 
    50                  55                  60                  


Thr Gly Ser Asn Ser Gln Trp Leu Thr Val Pro Ala His Asp Tyr Ala 
65                  70                  75                  80  


Tyr Phe Lys Ile Pro Val His Thr Asn Leu Trp Arg His Met Lys Val 
                85                  90                  95      


Val Leu Arg Met Leu Glu Asn Pro Asp Gln Pro Ile His Tyr Ala Leu 
            100                 105                 110         


His Ala Glu Val Lys Thr Gly Leu Met Phe Ser Lys Lys Ile Asn Ile 
        115                 120                 125             


Leu Arg His Gly Asp Ile Ile Pro Gly Asp Tyr Ile Arg Glu His His 
    130                 135                 140                 


His His His His 
145             


<210>  7
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WHy forward primer nucleotide sequence

<400>  7
gccaccatgg gatacttggc tactatc                                           27


<210>  8
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Truncated WHy forward primer nucleotide sequence

<400>  8
gccaccatgg gatgtgcttc atcag                                             25


<210>  9
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WHy reverse primer nucleotide sequence

<400>  9
tcaatggtga tgatggtggt gctcc                                             25


<210>  10
<211>  453
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nicotiana Benthamiana codon-optimised WHy nucleotide sequence

<400>  10
gccaccatgg gatgtgcttc atcaggaagt tcaggtttca aagatccaga tgtgcagtta       60

gttgatgtgg agcttattca cgcaaagtta ttggagcaac agtttatgct tcacttcaga      120

gttgataacc ctaactctaa gtcacttcca atgaggggta tggattacag gatccttttg      180

aataacactc ctcttgctac aggatctaat tcacaatggt tgactgttcc tgctcatgat      240

tacgcttact tcaagatccc agttcataca aatttgtgga gacatatgaa agttgttctt      300

aggatgttgg aaaatcctga tcagccaatt cattacgctc ttcatgctga ggttaaaact      360

ggtttgatgt tcagtaagaa gattaacatt ttgagacacg gagatattat tccaggagat      420

tacattaggg agcaccacca tcatcaccat tga                                   453


<210>  11
<211>  10
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Kozak sequence

<400>  11
gccaccatgg                                                              10


