                         SEQUENCE LISTING

<110>  Firmenich SA
 
<120>  METHOD FOR IDENTIFYING POSITIVE ALLOSTERIC MODULATORS FOR ODORANT
        RECEPTORS

<130>  10590/WO

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  937
<212>  DNA
<213>  Homo sapiens

<400>  1
atgactgggg gaggaaatat tacagaaatc acctatttca tcctgctggg attctcagat       60

tttcccagga tcataaaagt gctcttcact atattcctgg tgatctacat tacatctctg      120

gcctggaacc tctccctcat tgttttaata aggatggatt cccacctcca tacacccatg      180

tatttcttcc tcagtaacct gtccttcata gatgtctgct atatcagctc cacagtcccc      240

aagatgctct ccaacctctt acaggaacag caaactatca cttttgttgg ttgtattatt      300

cagtacttta tcttttcaac gatgggactg agtgagtctt gtctcatgac agccatggct      360

tatgatcgtt atgctgccat ttgtaacccc ctgctctatt catccatcat gtcacccacc      420

ctctgtgttt ggatggtact gggagcctac atgactggcc tcactgcttc tttattccaa      480

attggtgctt tgcttcaact ccacttctgt gggtctaatg tcatcagaca tttcttctgt      540

gacatgcccc aactgttaat cttgtcctgt actgacactt tctttgtaca ggtcatgact      600

gctatattaa ccatgttctt tgggatagca agtgccctag ttatcatgat atcctatggc      660

tatattggca tctccatcat gaagatcact tcagctaaag gcaggtccaa ggcattcaac      720

acctgtgctt ctcatctaac agctgtttcc ctcttctata catcaggaat ctttgtctat      780

ttgagttcca gctctggagg ttcttcaagc tttgacagat ttgcatctgt tttctacact      840

gtggtcattc ccatgttaaa tcccttgatt tacagtttga ggaacaaaga aattaaagat      900

gccttaaaga ggttgcaaaa gagaaagtgc tgctgag                               937


<210>  2
<211>  311
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Thr Gly Gly Gly Asn Ile Thr Glu Ile Thr Tyr Phe Ile Leu Leu 
1               5                   10                  15      


Gly Phe Ser Asp Phe Pro Arg Ile Ile Lys Val Leu Phe Thr Ile Phe 
            20                  25                  30          


Leu Val Ile Tyr Ile Thr Ser Leu Ala Trp Asn Leu Ser Leu Ile Val 
        35                  40                  45              


Leu Ile Arg Met Asp Ser His Leu His Thr Pro Met Tyr Phe Phe Leu 
    50                  55                  60                  


Ser Asn Leu Ser Phe Ile Asp Val Cys Tyr Ile Ser Ser Thr Val Pro 
65                  70                  75                  80  


Lys Met Leu Ser Asn Leu Leu Gln Glu Gln Gln Thr Ile Thr Phe Val 
                85                  90                  95      


Gly Cys Ile Ile Gln Tyr Phe Ile Phe Ser Thr Met Gly Leu Ser Glu 
            100                 105                 110         


Ser Cys Leu Met Thr Ala Met Ala Tyr Asp Arg Tyr Ala Ala Ile Cys 
        115                 120                 125             


Asn Pro Leu Leu Tyr Ser Ser Ile Met Ser Pro Thr Leu Cys Val Trp 
    130                 135                 140                 


Met Val Leu Gly Ala Tyr Met Thr Gly Leu Thr Ala Ser Leu Phe Gln 
145                 150                 155                 160 


Ile Gly Ala Leu Leu Gln Leu His Phe Cys Gly Ser Asn Val Ile Arg 
                165                 170                 175     


His Phe Phe Cys Asp Met Pro Gln Leu Leu Ile Leu Ser Cys Thr Asp 
            180                 185                 190         


Thr Phe Phe Val Gln Val Met Thr Ala Ile Leu Thr Met Phe Phe Gly 
        195                 200                 205             


Ile Ala Ser Ala Leu Val Ile Met Ile Ser Tyr Gly Tyr Ile Gly Ile 
    210                 215                 220                 


Ser Ile Met Lys Ile Thr Ser Ala Lys Gly Arg Ser Lys Ala Phe Asn 
225                 230                 235                 240 


Thr Cys Ala Ser His Leu Thr Ala Val Ser Leu Phe Tyr Thr Ser Gly 
                245                 250                 255     


Ile Phe Val Tyr Leu Ser Ser Ser Ser Gly Gly Ser Ser Ser Phe Asp 
            260                 265                 270         


Arg Phe Ala Ser Val Phe Tyr Thr Val Val Ile Pro Met Leu Asn Pro 
        275                 280                 285             


Leu Ile Tyr Ser Leu Arg Asn Lys Glu Ile Lys Asp Ala Leu Lys Arg 
    290                 295                 300                 


Leu Gln Lys Arg Lys Cys Cys 
305                 310     


<210>  3
<211>  930
<212>  DNA
<213>  Homo sapiens

<400>  3
atgaagagaa agaacttcac agaagtgtca gaattcattt tcttgggatt ttctagcttt       60

ggaaagcatc agataaccct ctttgtggtt ttcctaactg tctacatttt aactctggtt      120

gctaacatca tcattgtgac tatcatctgc attgaccatc atctccacac tcccatgtat      180

ttcttcctaa gcatgctggc tagttcagag acggtgtaca cactggtcat tgtgccacga      240

atgcttttga gcctcatttt tcataaccaa cctatctcct tggcaggctg tgctacacaa      300

atgttctttt ttgttatctt ggccactaat aattgcttcc tgcttactgc aatggggtat      360

gaccgctatg tggccatctg cagacccctg agatacactg tcatcatgag caagggacta      420

tgtgcccagc tggtgtgtgg gtcctttggc attggtctga ctatggcagt tctccatgtg      480

acagccatgt tcaatttgcc gttctgtggc acagtggtag accacttctt ttgtgacatt      540

tacccagtca tgaaactttc ttgcattgat accactatca atgagataat aaattatggt      600

gtaagttcat ttgtgatttt tgtgcccata ggcctgatat ttatctccta tgtccttgtc      660

atctcttcca tccttcaaat tgcctcagct gagggctgga agaagacctt tgccacctgt      720

gtctcccacc tcactgtggt tattgtccac tgtggctgtg cctccattgc ctacctcaag      780

ccgaagtcag aaagttcaat agaaaaagac cttgttctct cagtgacgta caccatcatc      840

actcccttgc tgaaccctgt tgtttacagt ctgagaaaca aggaggtaaa ggatgcccta      900

tgcagagttg tgggcagaaa tatttcttaa                                       930


<210>  4
<211>  309
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Lys Arg Lys Asn Phe Thr Glu Val Ser Glu Phe Ile Phe Leu Gly 
1               5                   10                  15      


Phe Ser Ser Phe Gly Lys His Gln Ile Thr Leu Phe Val Val Phe Leu 
            20                  25                  30          


Thr Val Tyr Ile Leu Thr Leu Val Ala Asn Ile Ile Ile Val Thr Ile 
        35                  40                  45              


Ile Cys Ile Asp His His Leu His Thr Pro Met Tyr Phe Phe Leu Ser 
    50                  55                  60                  


Met Leu Ala Ser Ser Glu Thr Val Tyr Thr Leu Val Ile Val Pro Arg 
65                  70                  75                  80  


Met Leu Leu Ser Leu Ile Phe His Asn Gln Pro Ile Ser Leu Ala Gly 
                85                  90                  95      


Cys Ala Thr Gln Met Phe Phe Phe Val Ile Leu Ala Thr Asn Asn Cys 
            100                 105                 110         


Phe Leu Leu Thr Ala Met Gly Tyr Asp Arg Tyr Val Ala Ile Cys Arg 
        115                 120                 125             


Pro Leu Arg Tyr Thr Val Ile Met Ser Lys Gly Leu Cys Ala Gln Leu 
    130                 135                 140                 


Val Cys Gly Ser Phe Gly Ile Gly Leu Thr Met Ala Val Leu His Val 
145                 150                 155                 160 


Thr Ala Met Phe Asn Leu Pro Phe Cys Gly Thr Val Val Asp His Phe 
                165                 170                 175     


Phe Cys Asp Ile Tyr Pro Val Met Lys Leu Ser Cys Ile Asp Thr Thr 
            180                 185                 190         


Ile Asn Glu Ile Ile Asn Tyr Gly Val Ser Ser Phe Val Ile Phe Val 
        195                 200                 205             


Pro Ile Gly Leu Ile Phe Ile Ser Tyr Val Leu Val Ile Ser Ser Ile 
    210                 215                 220                 


Leu Gln Ile Ala Ser Ala Glu Gly Trp Lys Lys Thr Phe Ala Thr Cys 
225                 230                 235                 240 


Val Ser His Leu Thr Val Val Ile Val His Cys Gly Cys Ala Ser Ile 
                245                 250                 255     


Ala Tyr Leu Lys Pro Lys Ser Glu Ser Ser Ile Glu Lys Asp Leu Val 
            260                 265                 270         


Leu Ser Val Thr Tyr Thr Ile Ile Thr Pro Leu Leu Asn Pro Val Val 
        275                 280                 285             


Tyr Ser Leu Arg Asn Lys Glu Val Lys Asp Ala Leu Cys Arg Val Val 
    290                 295                 300                 


Gly Arg Asn Ile Ser 
305                 


<210>  5
<211>  948
<212>  DNA
<213>  Mus musculus

<400>  5
atgcctggag ggaggaatag cacagtcatc accaagttca tccttgtggg attctcagat       60

tttccaaagc tcaagctggt tctctttgtt atcttcctgg gaagttatct ctccacagtg      120

gtgtggaact tgggcctcat catcttgatt aggattgacc cttacctaca cacacctatg      180

tacttcttcc tcagcaattt gtcattttta gatttctgtt acatttcatc tacaacccct      240

aaaatgctct cgggattctt ccagaagtct aaatctatct cctttgttgg gtgcaccatg      300

cagtacttca tcttctcaag cctgggtctg tccgaatgct gccttctggc agccatggct      360

tatgaccggt atgctgccat ttgtaatcct cttctctaca cagccatcat gtccccgtca      420

ctctgtgtgc acatggtggt tggagcctat agtactggtc tcttgggttc attgattcaa      480

ctgtgtgcta tacttcagct ccatttctgt gggccaaata ttataaacca tttcttttgt      540

gacctgcctc agctattagt tctttcctgc tctgaaacct ttcccctgca agtcttgaaa      600

tttgtaatag cagtgatttt tggggtggca tctgtcattg ttatcctgat atcctatggt      660

tatatcattg gcacaatcct gaatatcagc tcagtagaag gtaggtccaa ggcattcaat      720

acctgtgcct ctcacctgac agcagtcacc ctcttttttg gatcaggact ctttgtctat      780

atgcgcccca gctccaacag ttcccagggt tatgacaaga tggcttccgt gttctataca      840

gtggtgattc ccatgttgaa tcctctgatt tatagtctca ggaacaagga aataaaagat      900

gctcttcaga gatgtaaaaa taagtgcttt tctcagtgcc actgttag                   948


<210>  6
<211>  315
<212>  PRT
<213>  Mus musculus

<400>  6

Met Pro Gly Gly Arg Asn Ser Thr Val Ile Thr Lys Phe Ile Leu Val 
1               5                   10                  15      


Gly Phe Ser Asp Phe Pro Lys Leu Lys Leu Val Leu Phe Val Ile Phe 
            20                  25                  30          


Leu Gly Ser Tyr Leu Ser Thr Val Val Trp Asn Leu Gly Leu Ile Ile 
        35                  40                  45              


Leu Ile Arg Ile Asp Pro Tyr Leu His Thr Pro Met Tyr Phe Phe Leu 
    50                  55                  60                  


Ser Asn Leu Ser Phe Leu Asp Phe Cys Tyr Ile Ser Ser Thr Thr Pro 
65                  70                  75                  80  


Lys Met Leu Ser Gly Phe Phe Gln Lys Ser Lys Ser Ile Ser Phe Val 
                85                  90                  95      


Gly Cys Thr Met Gln Tyr Phe Ile Phe Ser Ser Leu Gly Leu Ser Glu 
            100                 105                 110         


Cys Cys Leu Leu Ala Ala Met Ala Tyr Asp Arg Tyr Ala Ala Ile Cys 
        115                 120                 125             


Asn Pro Leu Leu Tyr Thr Ala Ile Met Ser Pro Ser Leu Cys Val His 
    130                 135                 140                 


Met Val Val Gly Ala Tyr Ser Thr Gly Leu Leu Gly Ser Leu Ile Gln 
145                 150                 155                 160 


Leu Cys Ala Ile Leu Gln Leu His Phe Cys Gly Pro Asn Ile Ile Asn 
                165                 170                 175     


His Phe Phe Cys Asp Leu Pro Gln Leu Leu Val Leu Ser Cys Ser Glu 
            180                 185                 190         


Thr Phe Pro Leu Gln Val Leu Lys Phe Val Ile Ala Val Ile Phe Gly 
        195                 200                 205             


Val Ala Ser Val Ile Val Ile Leu Ile Ser Tyr Gly Tyr Ile Ile Gly 
    210                 215                 220                 


Thr Ile Leu Asn Ile Ser Ser Val Glu Gly Arg Ser Lys Ala Phe Asn 
225                 230                 235                 240 


Thr Cys Ala Ser His Leu Thr Ala Val Thr Leu Phe Phe Gly Ser Gly 
                245                 250                 255     


Leu Phe Val Tyr Met Arg Pro Ser Ser Asn Ser Ser Gln Gly Tyr Asp 
            260                 265                 270         


Lys Met Ala Ser Val Phe Tyr Thr Val Val Ile Pro Met Leu Asn Pro 
        275                 280                 285             


Leu Ile Tyr Ser Leu Arg Asn Lys Glu Ile Lys Asp Ala Leu Gln Arg 
    290                 295                 300                 


Cys Lys Asn Lys Cys Phe Ser Gln Cys His Cys 
305                 310                 315 


<210>  7
<211>  930
<212>  DNA
<213>  Mus musculus

<400>  7
atgcagagaa ataacttcac tgaagtgata gagttcgtct tcctgggatt ctccagcttt       60

ggaaagcatc agataaccct ctttgtggtt ttcctaacca tctacatttt aactctggct      120

ggcaacatca ttatagtgac aatcacacac atagaccacc accttcacac tcccatgtac      180

ttctttctga gcatgttggc aagctcagag actgtgtaca cactggtcat tgtcccacga      240

atgctttcca gcctgatttt ttacaacctt cccatatcct tggcaggctg cgcaacccaa      300

atgttctttt ttgtcacctt ggccaccaac aactgctttc tgctcacagc aatgggttat      360

gatcgttatg tggctatttg taatcctctg agatatacaa tcatcatgag caagggaatg      420

tgtgccttgt tggtttgtgg gtctttaggc actggcctgg ttatggcagt tcttcatgtg      480

ccagccatgt tccatttgcc cttttgtggc acggtggtgg agcacttttt ctgtgacata      540

tacccagtaa tgaagctttc ttgtgttgat accactgtca atgagataat caattatggt      600

gtaagttcat ttgtaattct tgtgcccata gggctgatat ttatctccta tgtgctcatt      660

gtctcttcca tccttaaaat tgtgtccact gaaggccaga agaaagcctt tgccacctgt      720

gcctctcatc tcactgtggt cattgtccac tatggctgtg cctccattgc ctacctcaaa      780

cccaaatcag aaagttcagt agaaaaagac cttcttctct ctgtgaccta cactatcatc      840

actcccttgc tgaaccctgt tgtctacagc ctcaggaaca aagaagtcaa agatgctcta      900

tgcagagctg tgggcagaaa cacttcttaa                                       930


<210>  8
<211>  309
<212>  PRT
<213>  Mus musculus

<400>  8

Met Gln Arg Asn Asn Phe Thr Glu Val Ile Glu Phe Val Phe Leu Gly 
1               5                   10                  15      


Phe Ser Ser Phe Gly Lys His Gln Ile Thr Leu Phe Val Val Phe Leu 
            20                  25                  30          


Thr Ile Tyr Ile Leu Thr Leu Ala Gly Asn Ile Ile Ile Val Thr Ile 
        35                  40                  45              


Thr His Ile Asp His His Leu His Thr Pro Met Tyr Phe Phe Leu Ser 
    50                  55                  60                  


Met Leu Ala Ser Ser Glu Thr Val Tyr Thr Leu Val Ile Val Pro Arg 
65                  70                  75                  80  


Met Leu Ser Ser Leu Ile Phe Tyr Asn Leu Pro Ile Ser Leu Ala Gly 
                85                  90                  95      


Cys Ala Thr Gln Met Phe Phe Phe Val Thr Leu Ala Thr Asn Asn Cys 
            100                 105                 110         


Phe Leu Leu Thr Ala Met Gly Tyr Asp Arg Tyr Val Ala Ile Cys Asn 
        115                 120                 125             


Pro Leu Arg Tyr Thr Ile Ile Met Ser Lys Gly Met Cys Ala Leu Leu 
    130                 135                 140                 


Val Cys Gly Ser Leu Gly Thr Gly Leu Val Met Ala Val Leu His Val 
145                 150                 155                 160 


Pro Ala Met Phe His Leu Pro Phe Cys Gly Thr Val Val Glu His Phe 
                165                 170                 175     


Phe Cys Asp Ile Tyr Pro Val Met Lys Leu Ser Cys Val Asp Thr Thr 
            180                 185                 190         


Val Asn Glu Ile Ile Asn Tyr Gly Val Ser Ser Phe Val Ile Leu Val 
        195                 200                 205             


Pro Ile Gly Leu Ile Phe Ile Ser Tyr Val Leu Ile Val Ser Ser Ile 
    210                 215                 220                 


Leu Lys Ile Val Ser Thr Glu Gly Gln Lys Lys Ala Phe Ala Thr Cys 
225                 230                 235                 240 


Ala Ser His Leu Thr Val Val Ile Val His Tyr Gly Cys Ala Ser Ile 
                245                 250                 255     


Ala Tyr Leu Lys Pro Lys Ser Glu Ser Ser Val Glu Lys Asp Leu Leu 
            260                 265                 270         


Leu Ser Val Thr Tyr Thr Ile Ile Thr Pro Leu Leu Asn Pro Val Val 
        275                 280                 285             


Tyr Ser Leu Arg Asn Lys Glu Val Lys Asp Ala Leu Cys Arg Ala Val 
    290                 295                 300                 


Gly Arg Asn Thr Ser 
305                 


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FLAG tag

<400>  9
gattacaagg acgacgacga taag                                              24


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG tag

<400>  10

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  11
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rho tag

<400>  11
atgaacggga ccgagggccc aaacttctac gtgcctttct ccaacaagac gggcgtggtg       60


<210>  12
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rho tag

<400>  12

Met Asn Gly Thr Glu Gly Pro Asn Phe Tyr Val Pro Phe Ser Asn Lys 
1               5                   10                  15      


Thr Gly Val Val 
            20  


<210>  13
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lucy tag

<400>  13
atgagacccc agatcctgct gctcctggcc ctgctgaccc taggcctggc t                51


<210>  14
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lucy tag

<400>  14

Met Arg Pro Gln Ile Leu Leu Leu Leu Ala Leu Leu Thr Leu Gly Leu 
1               5                   10                  15      


Ala 
    


<210>  15
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Motif

<400>  15

Met Ala Tyr Asp Arg Tyr Val Ala Ile Cys 
1               5                   10  


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Motif

<400>  16

Phe Ser Thr Cys Ser Ser His 
1               5           


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Motif

<400>  17

Pro Met Leu Asn Pro Phe Ile Tyr 
1               5               


