                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals SA
 
<120>  S aureus antigens and immunogenic compositions

<130>  VB66308 WO

<150>  GB1721582.3
<151>  2017-12-21

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  560
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Met Asn Met Lys Lys Lys Glu Lys His Ala Ile Arg Lys Lys Ser Ile 
1               5                   10                  15      


Gly Val Ala Ser Val Leu Val Gly Thr Leu Ile Gly Phe Gly Leu Leu 
            20                  25                  30          


Ser Ser Lys Glu Ala Asp Ala Ser Glu Asn Ser Val Thr Gln Ser Asp 
        35                  40                  45              


Ser Ala Ser Asn Glu Ser Lys Ser Asn Asp Ser Ser Ser Val Ser Ala 
    50                  55                  60                  


Ala Pro Lys Thr Asp Asp Thr Asn Val Ser Asp Thr Lys Thr Ser Ser 
65                  70                  75                  80  


Asn Thr Asn Asn Gly Glu Thr Ser Val Ala Gln Asn Pro Ala Gln Gln 
                85                  90                  95      


Glu Thr Thr Gln Ser Ser Ser Thr Asn Ala Thr Thr Glu Glu Thr Pro 
            100                 105                 110         


Val Thr Gly Glu Ala Thr Thr Thr Thr Thr Asn Gln Ala Asn Thr Pro 
        115                 120                 125             


Ala Thr Thr Gln Ser Ser Asn Thr Asn Ala Glu Glu Leu Val Asn Gln 
    130                 135                 140                 


Thr Ser Asn Glu Thr Thr Phe Asn Asp Thr Asn Thr Val Ser Ser Val 
145                 150                 155                 160 


Asn Ser Pro Gln Asn Ser Thr Asn Ala Glu Asn Val Ser Thr Thr Gln 
                165                 170                 175     


Asp Thr Ser Thr Glu Ala Thr Pro Ser Asn Asn Glu Ser Ala Pro Gln 
            180                 185                 190         


Ser Thr Asp Ala Ser Asn Lys Asp Val Val Asn Gln Ala Val Asn Thr 
        195                 200                 205             


Ser Ala Pro Arg Met Arg Ala Phe Ser Leu Ala Ala Val Ala Ala Asp 
    210                 215                 220                 


Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu Thr Asn Val Thr 
225                 230                 235                 240 


Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His Gln Ala Gly Tyr 
                245                 250                 255     


Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser Ala Val Lys Gly 
            260                 265                 270         


Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn Leu Asn Gly Val 
        275                 280                 285             


Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly Asp Gln Val Leu 
    290                 295                 300                 


Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile Tyr Thr Phe Thr 
305                 310                 315                 320 


Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr Leu Thr Met Pro 
                325                 330                 335     


Ala Tyr Ile Asp Pro Glu Asn Val Lys Lys Thr Gly Asn Val Thr Leu 
            340                 345                 350         


Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr Val Leu Val Asp 
        355                 360                 365             


Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile Lys Gly Thr Ile 
    370                 375                 380                 


Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln Thr Ile Tyr Val 
385                 390                 395                 400 


Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu Thr Gly Asn Leu 
                405                 410                 415     


Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln Gln Asn Thr Ser 
            420                 425                 430         


Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu Ser Glu Ser Tyr 
        435                 440                 445             


Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn Ser Val Asn Ile 
    450                 455                 460                 


Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe Asn Thr Pro Asp 
465                 470                 475                 480 


Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn Gly His Ile Asp 
                485                 490                 495     


Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr Leu Tyr Gly Tyr 
            500                 505                 510         


Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp Asn Glu Val Ala 
        515                 520                 525             


Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp Lys Pro Val Val 
    530                 535                 540                 


Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro Ile Pro Glu Asp 
545                 550                 555                 560 


<210>  2
<211>  522
<212>  PRT
<213>  Staphylococcus aureus

<400>  2

Ala Ser Glu Asn Ser Val Thr Gln Ser Asp Ser Ala Ser Asn Glu Ser 
1               5                   10                  15      


Lys Ser Asn Asp Ser Ser Ser Val Ser Ala Ala Pro Lys Thr Asp Asp 
            20                  25                  30          


Thr Asn Val Ser Asp Thr Lys Thr Ser Ser Asn Thr Asn Asn Gly Glu 
        35                  40                  45              


Thr Ser Val Ala Gln Asn Pro Ala Gln Gln Glu Thr Thr Gln Ser Ser 
    50                  55                  60                  


Ser Thr Asn Ala Thr Thr Glu Glu Thr Pro Val Thr Gly Glu Ala Thr 
65                  70                  75                  80  


Thr Thr Thr Thr Asn Gln Ala Asn Thr Pro Ala Thr Thr Gln Ser Ser 
                85                  90                  95      


Asn Thr Asn Ala Glu Glu Leu Val Asn Gln Thr Ser Asn Glu Thr Thr 
            100                 105                 110         


Phe Asn Asp Thr Asn Thr Val Ser Ser Val Asn Ser Pro Gln Asn Ser 
        115                 120                 125             


Thr Asn Ala Glu Asn Val Ser Thr Thr Gln Asp Thr Ser Thr Glu Ala 
    130                 135                 140                 


Thr Pro Ser Asn Asn Glu Ser Ala Pro Gln Ser Thr Asp Ala Ser Asn 
145                 150                 155                 160 


Lys Asp Val Val Asn Gln Ala Val Asn Thr Ser Ala Pro Arg Met Arg 
                165                 170                 175     


Ala Phe Ser Leu Ala Ala Val Ala Ala Asp Ala Pro Ala Ala Gly Thr 
            180                 185                 190         


Asp Ile Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly 
        195                 200                 205             


Thr Thr Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly 
    210                 215                 220                 


Phe Ser Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr 
225                 230                 235                 240 


Val Pro Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val 
                245                 250                 255     


Pro Pro Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp 
            260                 265                 270         


Ser Asp Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys 
        275                 280                 285             


Asp Asp Val Lys Ala Thr Leu Thr Met Pro Ala Tyr Ile Asp Pro Glu 
    290                 295                 300                 


Asn Val Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser 
305                 310                 315                 320 


Thr Thr Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys 
                325                 330                 335     


Phe Tyr Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr 
            340                 345                 350         


Asn Asn Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn 
        355                 360                 365             


Val Ile Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser 
    370                 375                 380                 


Asn Ala Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val 
385                 390                 395                 400 


Asp Asn Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn 
                405                 410                 415     


Phe Glu Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn 
            420                 425                 430         


Gln Tyr Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro 
        435                 440                 445             


Tyr Ile Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp 
    450                 455                 460                 


Leu Ala Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp 
465                 470                 475                 480 


Arg Ser Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly 
                485                 490                 495     


Ser Gly Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu 
            500                 505                 510         


Pro Gly Glu Ile Glu Pro Ile Pro Glu Asp 
        515                 520         


<210>  3
<211>  340
<212>  PRT
<213>  Staphylococcus aureus

<400>  3

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Pro Ala Tyr Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Ile Pro Glu Asp 
            340 


<210>  4
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A

<400>  4

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Ile Pro Glu Asp 
            340 


<210>  5
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A with N-terminal  S and Flgl signal sequence

<400>  5

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile 
            20                  25                  30          


Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr 
        35                  40                  45              


Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser 
    50                  55                  60                  


Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro 
65                  70                  75                  80  


Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro 
                85                  90                  95      


Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp 
            100                 105                 110         


Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp 
        115                 120                 125             


Val Lys Ala Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val 
    130                 135                 140                 


Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr 
145                 150                 155                 160 


Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr 
                165                 170                 175     


Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn 
            180                 185                 190         


Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile 
        195                 200                 205             


Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala 
    210                 215                 220                 


Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn 
225                 230                 235                 240 


Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu 
                245                 250                 255     


Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr 
            260                 265                 270         


Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile 
        275                 280                 285             


Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala 
    290                 295                 300                 


Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser 
305                 310                 315                 320 


Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly 
                325                 330                 335     


Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly 
            340                 345                 350         


Glu Ile Glu Pro Ile Pro Glu Asp 
        355                 360 


<210>  6
<211>  341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A with N-terminal  S

<400>  6

Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln 
1               5                   10                  15      


Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro 
            20                  25                  30          


His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn 
        35                  40                  45              


Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu 
    50                  55                  60                  


Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala 
65                  70                  75                  80  


Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val 
                85                  90                  95      


Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala 
            100                 105                 110         


Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr 
        115                 120                 125             


Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys 
    130                 135                 140                 


Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser 
145                 150                 155                 160 


Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg 
                165                 170                 175     


Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val 
            180                 185                 190         


Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp 
        195                 200                 205             


Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp 
    210                 215                 220                 


Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr 
225                 230                 235                 240 


Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu 
                245                 250                 255     


Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val 
            260                 265                 270         


Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser 
        275                 280                 285             


Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp 
    290                 295                 300                 


Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile 
305                 310                 315                 320 


Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu 
                325                 330                 335     


Pro Ile Pro Glu Asp 
            340     


<210>  7
<211>  528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild-type ClfAN1N2N3 with glycosite at position corresponding to 
       mutant 30 (I557 of full-length ClfA)

<400>  7

Ala Ser Glu Asn Ser Val Thr Gln Ser Asp Ser Ala Ser Asn Glu Ser 
1               5                   10                  15      


Lys Ser Asn Asp Ser Ser Ser Val Ser Ala Ala Pro Lys Thr Asp Asp 
            20                  25                  30          


Thr Asn Val Ser Asp Thr Lys Thr Ser Ser Asn Thr Asn Asn Gly Glu 
        35                  40                  45              


Thr Ser Val Ala Gln Asn Pro Ala Gln Gln Glu Thr Thr Gln Ser Ser 
    50                  55                  60                  


Ser Thr Asn Ala Thr Thr Glu Glu Thr Pro Val Thr Gly Glu Ala Thr 
65                  70                  75                  80  


Thr Thr Thr Thr Asn Gln Ala Asn Thr Pro Ala Thr Thr Gln Ser Ser 
                85                  90                  95      


Asn Thr Asn Ala Glu Glu Leu Val Asn Gln Thr Ser Asn Glu Thr Thr 
            100                 105                 110         


Phe Asn Asp Thr Asn Thr Val Ser Ser Val Asn Ser Pro Gln Asn Ser 
        115                 120                 125             


Thr Asn Ala Glu Asn Val Ser Thr Thr Gln Asp Thr Ser Thr Glu Ala 
    130                 135                 140                 


Thr Pro Ser Asn Asn Glu Ser Ala Pro Gln Ser Thr Asp Ala Ser Asn 
145                 150                 155                 160 


Lys Asp Val Val Asn Gln Ala Val Asn Thr Ser Ala Pro Arg Met Arg 
                165                 170                 175     


Ala Phe Ser Leu Ala Ala Val Ala Ala Asp Ala Pro Ala Ala Gly Thr 
            180                 185                 190         


Asp Ile Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly 
        195                 200                 205             


Thr Thr Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly 
    210                 215                 220                 


Phe Ser Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr 
225                 230                 235                 240 


Val Pro Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val 
                245                 250                 255     


Pro Pro Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp 
            260                 265                 270         


Ser Asp Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys 
        275                 280                 285             


Asp Asp Val Lys Ala Thr Leu Thr Met Pro Ala Tyr Ile Asp Pro Glu 
    290                 295                 300                 


Asn Val Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser 
305                 310                 315                 320 


Thr Thr Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys 
                325                 330                 335     


Phe Tyr Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr 
            340                 345                 350         


Asn Asn Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn 
        355                 360                 365             


Val Ile Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser 
    370                 375                 380                 


Asn Ala Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val 
385                 390                 395                 400 


Asp Asn Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn 
                405                 410                 415     


Phe Glu Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn 
            420                 425                 430         


Gln Tyr Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro 
        435                 440                 445             


Tyr Ile Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp 
    450                 455                 460                 


Leu Ala Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp 
465                 470                 475                 480 


Arg Ser Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly 
                485                 490                 495     


Ser Gly Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu 
            500                 505                 510         


Pro Gly Glu Ile Glu Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
        515                 520                 525             


<210>  8
<211>  528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN1N2N3 P298S/Y300A with glycosite at position corresponding 
       to mutant 30 (I557 of full-length ClfA)

<400>  8

Ala Ser Glu Asn Ser Val Thr Gln Ser Asp Ser Ala Ser Asn Glu Ser 
1               5                   10                  15      


Lys Ser Asn Asp Ser Ser Ser Val Ser Ala Ala Pro Lys Thr Asp Asp 
            20                  25                  30          


Thr Asn Val Ser Asp Thr Lys Thr Ser Ser Asn Thr Asn Asn Gly Glu 
        35                  40                  45              


Thr Ser Val Ala Gln Asn Pro Ala Gln Gln Glu Thr Thr Gln Ser Ser 
    50                  55                  60                  


Ser Thr Asn Ala Thr Thr Glu Glu Thr Pro Val Thr Gly Glu Ala Thr 
65                  70                  75                  80  


Thr Thr Thr Thr Asn Gln Ala Asn Thr Pro Ala Thr Thr Gln Ser Ser 
                85                  90                  95      


Asn Thr Asn Ala Glu Glu Leu Val Asn Gln Thr Ser Asn Glu Thr Thr 
            100                 105                 110         


Phe Asn Asp Thr Asn Thr Val Ser Ser Val Asn Ser Pro Gln Asn Ser 
        115                 120                 125             


Thr Asn Ala Glu Asn Val Ser Thr Thr Gln Asp Thr Ser Thr Glu Ala 
    130                 135                 140                 


Thr Pro Ser Asn Asn Glu Ser Ala Pro Gln Ser Thr Asp Ala Ser Asn 
145                 150                 155                 160 


Lys Asp Val Val Asn Gln Ala Val Asn Thr Ser Ala Pro Arg Met Arg 
                165                 170                 175     


Ala Phe Ser Leu Ala Ala Val Ala Ala Asp Ala Pro Ala Ala Gly Thr 
            180                 185                 190         


Asp Ile Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly 
        195                 200                 205             


Thr Thr Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly 
    210                 215                 220                 


Phe Ser Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr 
225                 230                 235                 240 


Val Pro Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val 
                245                 250                 255     


Pro Pro Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp 
            260                 265                 270         


Ser Asp Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys 
        275                 280                 285             


Asp Asp Val Lys Ala Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu 
    290                 295                 300                 


Asn Val Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser 
305                 310                 315                 320 


Thr Thr Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys 
                325                 330                 335     


Phe Tyr Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr 
            340                 345                 350         


Asn Asn Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn 
        355                 360                 365             


Val Ile Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser 
    370                 375                 380                 


Asn Ala Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val 
385                 390                 395                 400 


Asp Asn Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn 
                405                 410                 415     


Phe Glu Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn 
            420                 425                 430         


Gln Tyr Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro 
        435                 440                 445             


Tyr Ile Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp 
    450                 455                 460                 


Leu Ala Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp 
465                 470                 475                 480 


Arg Ser Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly 
                485                 490                 495     


Ser Gly Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu 
            500                 505                 510         


Pro Gly Glu Ile Glu Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
        515                 520                 525             


<210>  9
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild-type ClfAN2N3 with mutant 30 glycosite 

<400>  9

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Pro Ala Tyr Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
            340                 345     


<210>  10
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A  with mutant 30 glycosite

<400>  10

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
            340                 345     


<210>  11
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A with N-terminal  S and Flgl signal sequence 
       and mutant 30 glycosite 

<400>  11

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile 
            20                  25                  30          


Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr 
        35                  40                  45              


Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser 
    50                  55                  60                  


Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro 
65                  70                  75                  80  


Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro 
                85                  90                  95      


Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp 
            100                 105                 110         


Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp 
        115                 120                 125             


Val Lys Ala Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val 
    130                 135                 140                 


Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr 
145                 150                 155                 160 


Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr 
                165                 170                 175     


Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn 
            180                 185                 190         


Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile 
        195                 200                 205             


Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala 
    210                 215                 220                 


Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn 
225                 230                 235                 240 


Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu 
                245                 250                 255     


Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr 
            260                 265                 270         


Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile 
        275                 280                 285             


Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala 
    290                 295                 300                 


Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser 
305                 310                 315                 320 


Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly 
                325                 330                 335     


Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly 
            340                 345                 350         


Glu Ile Glu Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
        355                 360                 365     


<210>  12
<211>  347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A with N-terminal S and mutant 30 glycosite 
       

<400>  12

Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln 
1               5                   10                  15      


Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro 
            20                  25                  30          


His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn 
        35                  40                  45              


Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu 
    50                  55                  60                  


Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala 
65                  70                  75                  80  


Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val 
                85                  90                  95      


Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala 
            100                 105                 110         


Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr 
        115                 120                 125             


Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys 
    130                 135                 140                 


Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser 
145                 150                 155                 160 


Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg 
                165                 170                 175     


Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val 
            180                 185                 190         


Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp 
        195                 200                 205             


Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp 
    210                 215                 220                 


Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr 
225                 230                 235                 240 


Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu 
                245                 250                 255     


Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val 
            260                 265                 270         


Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser 
        275                 280                 285             


Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp 
    290                 295                 300                 


Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile 
305                 310                 315                 320 


Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu 
                325                 330                 335     


Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp 
            340                 345         


<210>  13
<211>  19
<212>  PRT
<213>  Escherichia coli

<400>  13

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala 
            


<210>  14
<211>  21
<212>  PRT
<213>  Escherichia coli

<400>  14

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  15
<211>  26
<212>  PRT
<213>  Escherichia coli

<400>  15

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210>  16
<211>  22
<212>  PRT
<213>  Erwinia carotovora

<400>  16

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210>  17
<211>  23
<212>  PRT
<213>  Escherichia coli

<400>  17

Met Ser Phe Lys Lys Ile Ile Lys Ala Phe Val Ile Met Ala Ala Leu 
1               5                   10                  15      


Val Ser Val Gln Ala His Ala 
            20              


<210>  18
<211>  19
<212>  PRT
<213>  Escherichia coli

<400>  18

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210>  19
<211>  21
<212>  PRT
<213>  Escherichia coli

<400>  19

Met Lys Gln Ala Leu Arg Val Ala Phe Gly Phe Leu Ile Leu Trp Ala 
1               5                   10                  15      


Ser Val Leu His Ala 
            20      


<210>  20
<211>  26
<212>  PRT
<213>  Escherichia coli

<400>  20

Met Lys Met Asn Lys Lys Val Leu Leu Thr Ser Thr Met Ala Ala Ser 
1               5                   10                  15      


Leu Leu Ser Val Ala Ser Val Gln Ala Ser 
            20                  25      


<210>  21
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosite


<220>  
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be Asp or Glu


<220>  
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>  
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid except proline


<220>  
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be Ser or Thr

<400>  21

Xaa Xaa Asn Xaa Xaa 
1               5           


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosite


<220>  
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be Asp or Glu


<220>  
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>  
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>  
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be Ser or Thr

<400>  22

Lys Xaa Xaa Asn Xaa Xaa Lys 
1               5                   


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   glycosite

<400>  23

Lys Asp Gln Asn Ala Thr Lys 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosite

<400>  24

Lys Asp Gln Asn Arg Thr Lys 
1               5           


<210>  25
<211>  354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A with mutant 30 glycosite, 
       additional GS and 6-His C-terminal tag

<400>  25

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp Gly Ser His His His His 
            340                 345                 350         


His His 
        


<210>  26
<211>  374
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A with N-terminal S and Flgl signal sequence 
       and mutant 30 glycosite, additional GS and 6-His 
       C-terminal tag

<400>  26

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile 
            20                  25                  30          


Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr 
        35                  40                  45              


Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser 
    50                  55                  60                  


Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro 
65                  70                  75                  80  


Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro 
                85                  90                  95      


Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp 
            100                 105                 110         


Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp 
        115                 120                 125             


Val Lys Ala Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val 
    130                 135                 140                 


Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr 
145                 150                 155                 160 


Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr 
                165                 170                 175     


Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn 
            180                 185                 190         


Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile 
        195                 200                 205             


Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala 
    210                 215                 220                 


Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn 
225                 230                 235                 240 


Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu 
                245                 250                 255     


Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr 
            260                 265                 270         


Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile 
        275                 280                 285             


Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala 
    290                 295                 300                 


Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser 
305                 310                 315                 320 


Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly 
                325                 330                 335     


Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly 
            340                 345                 350         


Glu Ile Glu Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp Gly Ser 
        355                 360                 365             


His His His His His His 
    370                 


<210>  27
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A with N-terminal S, glycosite, 
       additional GS and 6-His C-terminal tag

<400>  27

Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln 
1               5                   10                  15      


Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro 
            20                  25                  30          


His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn 
        35                  40                  45              


Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu 
    50                  55                  60                  


Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala 
65                  70                  75                  80  


Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val 
                85                  90                  95      


Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala 
            100                 105                 110         


Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr 
        115                 120                 125             


Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys 
    130                 135                 140                 


Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser 
145                 150                 155                 160 


Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg 
                165                 170                 175     


Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val 
            180                 185                 190         


Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp 
        195                 200                 205             


Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp 
    210                 215                 220                 


Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr 
225                 230                 235                 240 


Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu 
                245                 250                 255     


Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val 
            260                 265                 270         


Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser 
        275                 280                 285             


Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp 
    290                 295                 300                 


Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile 
305                 310                 315                 320 


Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu 
                325                 330                 335     


Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp Gly Ser His His His 
            340                 345                 350         


His His His 
        355 


<210>  28
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A mutant 1

<400>  28

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Lys Asp Gln Asn Ala Thr Lys Ser Gly 
            20                  25                  30          


Thr Thr Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly 
        35                  40                  45              


Phe Ser Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr 
    50                  55                  60                  


Val Pro Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val 
65                  70                  75                  80  


Pro Pro Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp 
                85                  90                  95      


Ser Asp Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys 
            100                 105                 110         


Asp Asp Val Lys Ala Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu 
        115                 120                 125             


Asn Val Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser 
    130                 135                 140                 


Thr Thr Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys 
145                 150                 155                 160 


Phe Tyr Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr 
                165                 170                 175     


Asn Asn Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn 
            180                 185                 190         


Val Ile Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser 
        195                 200                 205             


Asn Ala Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val 
    210                 215                 220                 


Asp Asn Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn 
225                 230                 235                 240 


Phe Glu Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn 
                245                 250                 255     


Gln Tyr Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro 
            260                 265                 270         


Tyr Ile Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp 
        275                 280                 285             


Leu Ala Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp 
    290                 295                 300                 


Arg Ser Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly 
305                 310                 315                 320 


Ser Gly Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu 
                325                 330                 335     


Pro Gly Glu Ile Glu Pro Ile Pro Glu Asp 
            340                 345     


<210>  29
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A mutant 27 

<400>  29

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Lys Asp Gln Asn Ala Thr Lys Pro Asp Glu 
                325                 330                 335     


Pro Gly Glu Ile Glu Pro Ile Pro Glu Asp 
            340                 345     


<210>  30
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A mutant 28 

<400>  30

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Lys Asp Gln Asn Ala Thr Lys Glu 
                325                 330                 335     


Pro Gly Glu Ile Glu Pro Ile Pro Glu Asp 
            340                 345     


<210>  31
<211>  346
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A mutant 29 

<400>  31

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Lys Asp Gln Asn Ala Thr 
                325                 330                 335     


Lys Gly Glu Ile Glu Pro Ile Pro Glu Asp 
            340                 345     


<210>  32
<211>  349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A with mutant 30 glycosite, 
       N-terminal S, C-terminal  GS

<400>  32

Ser Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln 
1               5                   10                  15      


Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro 
            20                  25                  30          


His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn 
        35                  40                  45              


Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu 
    50                  55                  60                  


Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala 
65                  70                  75                  80  


Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val 
                85                  90                  95      


Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala 
            100                 105                 110         


Thr Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr 
        115                 120                 125             


Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys 
    130                 135                 140                 


Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser 
145                 150                 155                 160 


Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg 
                165                 170                 175     


Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val 
            180                 185                 190         


Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp 
        195                 200                 205             


Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp 
    210                 215                 220                 


Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr 
225                 230                 235                 240 


Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu 
                245                 250                 255     


Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val 
            260                 265                 270         


Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser 
        275                 280                 285             


Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp 
    290                 295                 300                 


Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile 
305                 310                 315                 320 


Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu 
                325                 330                 335     


Pro Lys Asp Gln Asn Ala Thr Lys Pro Glu Asp Gly Ser 
            340                 345                 


