                         SEQUENCE LISTING

<110>  GlaxoSmithKlineBiologicals SA
 
<120>  IMMUNOGENIC COMPOSITION

<130>  VB66307 WO

<150>  GB1721576.5
<151>  2017-12-21

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  293
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  2
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H48C/G122C

<400>  2

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  3
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/H48C/G122C

<400>  3

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  4
<211>  313
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Hla H35L/H48C/G122C with N-terminal S and 
       Flgl signal sequence

<400>  4

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val 
145                 150                 155                 160 


Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile 
                165                 170                 175     


Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn 
            180                 185                 190         


Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn 
        195                 200                 205             


Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met 
    210                 215                 220                 


Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu 
225                 230                 235                 240 


Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg 
                245                 250                 255     


Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val 
            260                 265                 270         


Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr 
        275                 280                 285             


Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile 
    290                 295                 300                 


Asp Trp Glu Lys Glu Glu Met Thr Asn 
305                 310             


<210>  5
<211>  319
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Hla H35L/H48C/G122C with N-terminal S and 
       Flgl signal sequence and C-terminal GSHRHR

<400>  5

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val 
145                 150                 155                 160 


Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile 
                165                 170                 175     


Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn 
            180                 185                 190         


Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn 
        195                 200                 205             


Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met 
    210                 215                 220                 


Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu 
225                 230                 235                 240 


Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg 
                245                 250                 255     


Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val 
            260                 265                 270         


Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr 
        275                 280                 285             


Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile 
    290                 295                 300                 


Asp Trp Glu Lys Glu Glu Met Thr Asn Gly Ser His Arg His Arg 
305                 310                 315                 


<210>  6
<211>  300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Hla H35L/H48C/G122C with N-terminal S and 
       C-terminal GSHRHR

<400>  6

Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly 
1               5                   10                  15      


Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu 
            20                  25                  30          


Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn 
        35                  40                  45              


Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly 
    50                  55                  60                  


Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala 
65                  70                  75                  80  


Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val 
                85                  90                  95      


Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu 
            100                 105                 110         


Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp 
        115                 120                 125             


Asp Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly 
    130                 135                 140                 


His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser 
145                 150                 155                 160 


Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val 
                165                 170                 175     


Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr 
            180                 185                 190         


Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala 
        195                 200                 205             


Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly 
    210                 215                 220                 


Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser 
225                 230                 235                 240 


Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp 
                245                 250                 255     


Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys 
            260                 265                 270         


Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu 
        275                 280                 285             


Lys Glu Glu Met Thr Asn Gly Ser His Arg His Arg 
    290                 295                 300 


<210>  7
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/H48C/G122C with KDQNRTK 
       substitution for residue K131

<400>  7

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly Leu Ile Gly Ala 
    130                 135                 140                 


Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys 
145                 150                 155                 160 


Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile 
                165                 170                 175     


Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser 
            180                 185                 190         


Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly 
        195                 200                 205             


Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser 
    210                 215                 220                 


Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met 
225                 230                 235                 240 


Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu 
                245                 250                 255     


Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys 
            260                 265                 270         


Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr 
        275                 280                 285             


Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 


<210>  8
<211>  319
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Hla H35L/H48C/G122C with N-terminal S, 
       Flgl signal sequence, and KDQNRTK substitution for residue K131

<400>  8

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly 
145                 150                 155                 160 


Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln 
                165                 170                 175     


Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly 
            180                 185                 190         


Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr 
        195                 200                 205             


Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys 
    210                 215                 220                 


Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn 
225                 230                 235                 240 


Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr 
                245                 250                 255     


Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp 
            260                 265                 270         


Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser 
        275                 280                 285             


Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser 
    290                 295                 300                 


Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
305                 310                 315                 


<210>  9
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Hla H35L/H48C/G122C with N-terminal S, 
       Flgl signal sequence, C-terminal GSHRHR, and KDQNRTK substitution
       for residue K131

<400>  9

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly 
145                 150                 155                 160 


Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln 
                165                 170                 175     


Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly 
            180                 185                 190         


Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr 
        195                 200                 205             


Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys 
    210                 215                 220                 


Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn 
225                 230                 235                 240 


Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr 
                245                 250                 255     


Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp 
            260                 265                 270         


Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser 
        275                 280                 285             


Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser 
    290                 295                 300                 


Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn Gly 
305                 310                 315                 320 


Ser His Arg His Arg 
                325 


<210>  10
<211>  306
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Hla H35L/H48C/G122C with N-terminal S, 
       C-terminal GSHRHR, and KDQNRTK substitution for residue K131

<400>  10

Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly 
1               5                   10                  15      


Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu 
            20                  25                  30          


Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn 
        35                  40                  45              


Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly 
    50                  55                  60                  


Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala 
65                  70                  75                  80  


Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val 
                85                  90                  95      


Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu 
            100                 105                 110         


Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp 
        115                 120                 125             


Asp Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly Leu Ile Gly 
    130                 135                 140                 


Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe 
145                 150                 155                 160 


Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val 
                165                 170                 175     


Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp 
            180                 185                 190         


Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn 
        195                 200                 205             


Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser 
    210                 215                 220                 


Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr 
225                 230                 235                 240 


Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr 
                245                 250                 255     


Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp 
            260                 265                 270         


Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg 
        275                 280                 285             


Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn Gly Ser His Arg 
    290                 295                 300                 


His Arg 
305     



<210>  11
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosite


<220>  
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be Asp or Glu


<220>  
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>  
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid except proline


<220>  
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be Ser or Thr

<400>  21

Xaa Xaa Asn Xaa Xaa 
1               5           


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosite


<220>  
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be Asp or Glu


<220>  
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>  
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>  
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be Ser or Thr

<400>  22

1               5                   
Lys Asp Glu Xaa Asn Glx Ser Thr Lys 
1               5                   


<210>  13
<211>  19
<212>  PRT
<213>  Escherichia coli

<400>  13

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala 
            


<210>  14
<211>  21
<212>  PRT
<213>  Escherichia coli

<400>  14

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  15
<211>  26
<212>  PRT
<213>  Escherichia coli

<400>  15

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210>  16
<211>  22
<212>  PRT
<213>  Erwinia carotovora

<400>  16

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210>  17
<211>  23
<212>  PRT
<213>  Escherichia coli

<400>  17

Met Ser Phe Lys Lys Ile Ile Lys Ala Phe Val Ile Met Ala Ala Leu 
1               5                   10                  15      


Val Ser Val Gln Ala His Ala 
            20              


<210>  18
<211>  28
<212>  PRT
<213>  Bacillus subtilis

<400>  18

Met Phe Lys Phe Lys Lys Lys Phe Leu Val Gly Leu Thr Ala Ala Phe 
1               5                   10                  15      


Met Ser Ile Ser Met Phe Ser Ala Thr Ala Ser Ala 
            20                  25              


<210>  19
<211>  19
<212>  PRT
<213>  Escherichia coli

<400>  19

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210>  20
<211>  21
<212>  PRT
<213>  Escherichia coli

<400>  20

Met Lys Gln Ala Leu Arg Val Ala Phe Gly Phe Leu Ile Leu Trp Ala 
1               5                   10                  15      


Ser Val Leu His Ala 
            20      


<210>  21
<211>  26
<212>  PRT
<213>  Escherichia coli

<400>  21

Met Lys Met Asn Lys Lys Val Leu Leu Thr Ser Thr Met Ala Ala Ser 
1               5                   10                  15      


Leu Leu Ser Val Ala Ser Val Gln Ala Ser 
            20                  25      


<210>  22
<211>  305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/H48C/G122C with C-terminal
       GSHRHR and KDQNRTK substitution for residue K131

<400>  22

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly Leu Ile Gly Ala 
    130                 135                 140                 


Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys 
145                 150                 155                 160 


Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile 
                165                 170                 175     


Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser 
            180                 185                 190         


Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly 
        195                 200                 205             


Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser 
    210                 215                 220                 


Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met 
225                 230                 235                 240 


Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu 
                245                 250                 255     


Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys 
            260                 265                 270         


Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr 
        275                 280                 285             


Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn Gly Ser His Arg His 
    290                 295                 300                 


Arg 
305 


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KDQNRTK glycosite

<400>  23

Lys Asp Gln Asn Arg Thr Lys 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KDQNATK glycosite

<400>  24

Lys Asp Gln Asn Arg Thr Lys 
1               5           


<210>  25
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HRHR C-terminal tag

<400>  25

His Arg His Arg 
1               


<210>  26
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GSHRHR C-terminal tag

<400>  26

Gly Ser His Arg His Arg 
1               5       


<210>  27
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature Hla H35L/Y102C/G126C

<400>  27

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Cys Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Cys Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  28
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature HlaH35L/G122C/H48C

<400>  28

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  29
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature Hla H35L/G122C/L52C

<400>  29

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Cys Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  30
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature HlaH35L

<400>  30

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


