﻿                         序列表

<110>  江南大学

<120>  一种双酶偶联全细胞催化马来酸合成L-天冬氨酸的方法

<160>  25

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  250
<212>  PRT
<213>  粘质沙雷氏菌(Serratia marcescens)

<400>  1
Met Ser Asn His Tyr Arg Ile Gly Gln Ile Val Pro Ser Ser Asn Thr 
1               5                   10                  15      
Thr Met Glu Thr Glu Ile Pro Ala Met Leu Gly Ala Arg Gln Leu Ile 
            20                  25                  30          
Arg Pro Glu Arg Phe Thr Phe His Ser Ser Arg Met Arg Met Lys His 
        35                  40                  45              
Val Asn Lys Glu Glu Leu Ala Ala Met Asp Ala Glu Ser Asp Arg Cys 
    50                  55                  60                  
Ala Leu Glu Leu Ser Asp Ala Arg Val Asp Val Leu Gly Tyr Ala Cys 
65                  70                  75                  80  
Leu Val Ala Ile Met Ala Met Gly Leu Gly Tyr His Arg Glu Ser Gln 
                85                  90                  95      
Ala Arg Leu Ala Gln Val Thr Lys Asp Asn Gln Ala Ala Ala Pro Val 
            100                 105                 110         
Ile Ser Ser Ala Gly Ala Leu Val Asn Gly Leu Lys Val Ile Gly Ala 
        115                 120                 125             
Lys Arg Ile Ala Leu Val Ala Pro Tyr Met Lys Pro Leu Thr Gln Leu 
    130                 135                 140                 
Val Val Asp Tyr Ile Gln His Glu Gly Ile Glu Val Lys Val Trp Arg 
145                 150                 155                 160 
Ala Leu Glu Ile Pro Asp Asn Leu Asp Val Gly Arg His Asp Pro Ala 
                165                 170                 175     
Arg Leu Pro Gly Ile Val Ala Glu Met Asp Leu Arg Glu Val Asp Ala 
            180                 185                 190         
Ile Val Leu Ser Ala Cys Val Gln Met Pro Ser Leu Pro Ala Val Pro 
        195                 200                 205             
Thr Val Glu Ala Gln Thr Gly Lys Pro Val Ile Thr Ala Ala Ile Ala 
    210                 215                 220                 
Thr Thr Tyr Ala Met Leu Thr Ala Leu Glu Leu Glu Pro Ile Val Pro 
225                 230                 235                 240 
Gly Ala Gly Ala Leu Leu Ser Gly Ala Tyr 
                245                 250 

<210>  2
<211>  753
<212>  DNA
<213>  粘质沙雷氏菌(Serratia marcescens)

<400>  2
atgagcaacc actaccgcat cggccagatc gtgcccagct ccaacaccac gatggaaacc        60
gagatcccgg cgatgctggg cgcgcgccag ctgatacgcc cggagcgttt cacctttcac       120
tccagccgca tgcgcatgaa acacgtcaat aaagaagaat tggcggcgat ggacgccgag       180
tccgatcgct gcgcgctgga gctgtccgac gcgcgggtcg acgtgctcgg ctacgcctgc       240
ctggtggcca tcatggcgat ggggctgggc taccaccgcg aatcgcaggc ccggctggcg       300
caggtgacga aagacaatca ggccgccgcg ccggtcatca gcagcgccgg cgcgctggtc       360
aacggcctga aggtgatcgg cgccaaacgc atcgcgctgg tggcgcccta catgaaaccg       420
ctgacccagc tggtggtgga ctacatccag cacgaaggca tcgaggtcaa ggtatggcgc       480
gcgctggaga tcccggacaa cctcgacgtc ggccggcacg atccggccag gctgccgggg       540
atcgtcgccg agatggactt acgcgaggtc gatgctatcg tgctgtccgc ctgcgtgcag       600
atgccttcgc tgccggccgt cccgacggtg gaggcccaaa ccggcaaacc ggtgatcacc       660
gccgccatcg ccaccactta cgcgatgctg accgcgctgg agctggaacc gatcgttccc       720
ggcgccggcg ccctgctgtc cggcgcttat tga                                    753

<210>  3
<211>  478
<212>  PRT
<213>  Escherichia coli

<400>  3
Met Ser Asn Asn Ile Arg Ile Glu Glu Asp Leu Leu Gly Thr Arg Glu 
1               5                   10                  15      
Val Pro Ala Asp Ala Tyr Tyr Gly Val His Thr Leu Arg Ala Ile Val 
            20                  25                  30          
Asn Phe Tyr Ile Ser Asn Asn Lys Ile Ser Asp Ile Pro Glu Phe Val 
        35                  40                  45              
Arg Gly Met Val Met Val Lys Lys Ala Ala Ala Met Ala Asn Lys Glu 
    50                  55                  60                  
Leu Gln Thr Ile Pro Lys Ser Val Ala Asn Ala Ile Ile Ala Ala Cys 
65                  70                  75                  80  
Asp Glu Val Leu Asn Asn Gly Lys Cys Met Asp Gln Phe Pro Val Asp 
                85                  90                  95      
Val Tyr Gln Gly Gly Ala Gly Thr Ser Val Asn Met Asn Thr Asn Glu 
            100                 105                 110         
Val Leu Ala Asn Ile Gly Leu Glu Leu Met Gly His Gln Lys Gly Glu 
        115                 120                 125             
Tyr Gln Tyr Leu Asn Pro Asn Asp His Val Asn Lys Cys Gln Ser Thr 
    130                 135                 140                 
Asn Asp Ala Tyr Pro Thr Gly Phe Arg Ile Ala Val Tyr Ser Ser Leu 
145                 150                 155                 160 
Ile Lys Leu Val Asp Ala Ile Asn Gln Leu Arg Glu Gly Phe Glu Arg 
                165                 170                 175     
Lys Ala Val Glu Phe Gln Asp Ile Leu Lys Met Gly Arg Thr Gln Leu 
            180                 185                 190         
Gln Asp Ala Val Pro Met Thr Leu Gly Gln Glu Phe Arg Ala Phe Ser 
        195                 200                 205             
Ile Leu Leu Lys Glu Glu Val Lys Asn Ile Gln Arg Thr Ala Glu Leu 
    210                 215                 220                 
Leu Leu Glu Val Asn Leu Gly Ala Thr Ala Ile Gly Thr Gly Leu Asn 
225                 230                 235                 240 
Thr Pro Lys Glu Tyr Ser Pro Leu Ala Val Lys Lys Leu Ala Glu Val 
                245                 250                 255     
Thr Gly Phe Pro Cys Val Pro Ala Glu Asp Leu Ile Glu Ala Thr Ser 
            260                 265                 270         
Asp Cys Gly Ala Tyr Val Met Val His Gly Ala Leu Lys Arg Leu Ala 
        275                 280                 285             
Val Lys Met Ser Lys Ile Cys Asn Asp Leu Arg Leu Leu Ser Ser Gly 
    290                 295                 300                 
Pro Arg Ala Gly Leu Asn Glu Ile Asn Leu Pro Glu Leu Gln Ala Gly 
305                 310                 315                 320 
Ser Ser Ile Met Pro Ala Lys Val Asn Pro Val Val Pro Glu Val Val 
                325                 330                 335     
Asn Gln Val Cys Phe Lys Val Ile Gly Asn Asp Thr Thr Val Thr Met 
            340                 345                 350         
Ala Ala Glu Ala Gly Gln Leu Gln Leu Asn Val Met Glu Pro Val Ile 
        355                 360                 365             
Gly Gln Ala Met Phe Glu Ser Val His Ile Leu Thr Asn Ala Cys Tyr 
    370                 375                 380                 
Asn Leu Leu Glu Lys Cys Ile Asn Gly Ile Thr Ala Asn Lys Glu Val 
385                 390                 395                 400 
Cys Glu Gly Tyr Val Tyr Asn Ser Ile Gly Ile Val Thr Tyr Leu Asn 
                405                 410                 415     
Pro Phe Ile Gly His His Asn Gly Asp Ile Val Gly Lys Ile Cys Ala 
            420                 425                 430         
Glu Thr Gly Lys Ser Val Arg Glu Val Val Leu Glu Arg Gly Leu Leu 
        435                 440                 445             
Thr Glu Ala Glu Leu Asp Asp Ile Phe Ser Val Gln Asn Leu Met His 
    450                 455                 460                 
Pro Ala Tyr Lys Ala Lys Arg Tyr Thr Asp Glu Ser Glu Gln 
465                 470                 475             

<210>  4
<211>  1437
<212>  DNA
<213>  Escherichia coli

<400>  4
atgtcaaaca acattcgtat cgaagaagat ctgttgggta ccagggaagt tccagctgat        60
gcctactatg gtgttcacac tctgagagcg attgtaaact tctatatcag caacaacaaa       120
atcagtgata ttcctgaatt tgttcgcggt atggtaatgg ttaaaaaagc cgcagctatg       180
gcaaacaaag agctgcaaac cattcctaaa agtgtagcga atgccatcat tgccgcatgt       240
gatgaagtcc tgaacaacgg aaaatgcatg gatcagttcc cggtagacgt ctaccagggc       300
ggcgcaggta cttccgtaaa catgaacacc aacgaagtgc tggccaatat cggtctggaa       360
ctgatgggtc accaaaaagg tgaatatcag tacctgaacc cgaacgacca tgttaacaaa       420
tgtcagtcca ctaacgacgc ctacccgacc ggtttccgta tcgcagttta ctcttccctg       480
attaagctgg tagatgcgat taaccaactg cgtgaaggct ttgaacgtaa agctgtcgaa       540
ttccaggaca tcctgaaaat gggtcgtacc cagctgcagg acgcagtacc gatgaccctc       600
ggtcaggaat tccgcgcttt cagcatcctg ctgaaagaag aagtgaaaaa catccaacgt       660
accgctgaac tgctgctgga agttaacctt ggtgcaacag caatcggtac tggtctgaac       720
acgccgaaag agtactctcc gctggcagtg aaaaaactgg ctgaagttac tggcttccca       780
tgcgtaccgg ctgaagacct gatcgaagcg acctctgact gcggcgctta tgttatggtt       840
cacggcgcgc tgaaacgcct ggctgtgaag atgtccaaaa tctgtaacga cctgcgcttg       900
ctctcttcag gcccacgtgc cggcctgaac gagatcaacc tgccggaact gcaggcgggc       960
tcttccatca tgccagctaa agtaaacccg gttgttccgg aagtggttaa ccaggtatgc      1020
ttcaaagtca tcggtaacga caccactgtt accatggcag cagaagcagg tcagctgcag      1080
ttgaacgtta tggagccggt cattggccag gccatgttcg aatccgttca cattctgacc      1140
aacgcttgct acaacctgct ggaaaaatgc attaacggca tcactgctaa caaagaagtg      1200
tgcgaaggtt acgtttacaa ctctatcggt atcgttactt acctgaaccc gttcatcggt      1260
caccacaacg gtgacatcgt gggtaaaatc tgtgccgaaa ccggtaagag tgtacgtgaa      1320
gtcgttctgg aacgcggtct gttgactgaa gcggaacttg acgatatttt ctccgtacag      1380
aatctgatgc acccggctta caaagcaaaa cgctatactg atgaaagcga acagtaa         1437

<210>  5
<211>  32
<212>  DNA
<213>  人工序列

<400>  5
tttggatccg atgagcaacc actaccgcat cg                                      32

<210>  6
<211>  30
<212>  DNA
<213>  人工序列

<400>  6
tttaagcttt caataagcgc cggacagcag                                         30

<210>  7
<211>  28
<212>  DNA
<213>  人工序列

<400>  7
tttcatatga gcaaccacta ccgcatcg                                           28

<210>  8
<211>  30
<212>  DNA
<213>  人工序列

<400>  8
tttctcgagt caataagcgc cggacagcag                                         30

<210>  9
<211>  31
<212>  DNA
<213>  人工序列

<400>  9
tttaagctta tgagcaacca ctaccgcatc g                                       31

<210>  10
<211>  35
<212>  DNA
<213>  人工序列

<400>  10
tttggatccg atgtcaaaca acattcgtat cgaag                                   35

<210>  11
<211>  37
<212>  DNA
<213>  人工序列

<400>  11
tttaagcttt tactgttcgc tttcatcagt atagcgt                                 37

<210>  12
<211>  34
<212>  DNA
<213>  人工序列

<400>  12
tttcatatgt caaacaacat tcgtatcgaa gaag                                    34

<210>  13
<211>  37
<212>  DNA
<213>  人工序列

<400>  13
tttctcgagt tactgttcgc tttcatcagt atagcgt                                 37

<210>  14
<211>  34
<212>  DNA
<213>  人工序列

<400>  14
tttaagctta tgtcaaacaa cattcgtatc gaag                                    34

<210>  15
<211>  52
<212>  DNA
<213>  人工序列

<400>  15
cgaaaatccc taaggagctt aagcatgggc agcagccatc accatcatca cc                52

<210>  16
<211>  71
<212>  DNA
<213>  人工序列

<400>  16
gcttaagctc cttagggatt ttcgattaaa gttaaacaaa attatttcta caggggaatt        60
gttatccgct c                                                             71

<210>  17
<211>  55
<212>  DNA
<213>  人工序列

<400>  17
catcaccgtt agacgaggag gtatcctatg ggcagcagcc atcaccatca tcacc             55

<210>  18
<211>  74
<212>  DNA
<213>  人工序列

<400>  18
aggatacctc ctcgtctaac ggtgatgatt aaagttaaac aaaattattt ctacagggga        60
attgttatcc gctc                                                          74

<210>  19
<211>  51
<212>  DNA
<213>  人工序列

<400>  19
aataccctac taaggaggta agcatgggca gcagccatca ccatcatcac c                 51

<210>  20
<211>  70
<212>  DNA
<213>  人工序列

<400>  20
gcttacctcc ttagtagggt attattaaag ttaaacaaaa ttatttctac aggggaattg        60
ttatccgctc                                                               70

<210>  21
<211>  58
<212>  DNA
<213>  人工序列

<400>  21
gaactcgaac atagtcttaa ggaggttcaa atgggcagca gccatcacca tcatcacc          58

<210>  22
<211>  77
<212>  DNA
<213>  人工序列

<400>  22
ttgaacctcc ttaagactat gttcgagttc attaaagtta aacaaaatta tttctacagg        60
ggaattgtta tccgctc                                                       77

<210>  23
<211>  27
<212>  DNA
<213>  人工序列

<400>  23
catcaccgtt agacgaggag gtatcct                                            27

<210>  24
<211>  23
<212>  DNA
<213>  人工序列

<400>  24
aataccctac taaggaggta agc                                                23

<210>  25
<211>  30
<212>  DNA
<213>  人工序列

<400>  25
gaactcgaac atagtcttaa ggaggttcaa                                         30

