                         SEQUENCE LISTING

<110>  Glycosyn LLC
 
<120>  SIALYLTRANSFERASES AND USES THEREOF

<130>  037847-522001WO

<150>  US 62/559,481
<151>  2017-12-15

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  514
<212>  PRT
<213>  Photobacterium sp.

<400>  1

Met Lys Asn Phe Leu Leu Leu Thr Leu Ile Leu Leu Thr Ala Cys Asn 
1               5                   10                  15      


Asn Ser Glu Glu Asn Thr Gln Ser Ile Ile Lys Asn Asp Ile Asn Lys 
            20                  25                  30          


Thr Ile Ile Asp Glu Glu Tyr Val Asn Leu Glu Pro Ile Asn Gln Ser 
        35                  40                  45              


Asn Ile Ser Phe Thr Lys His Ser Trp Val Gln Thr Cys Gly Thr Gln 
    50                  55                  60                  


Gln Leu Leu Thr Glu Gln Asn Lys Glu Ser Ile Ser Leu Ser Val Val 
65                  70                  75                  80  


Ala Pro Arg Leu Asp Asp Asp Glu Lys Tyr Cys Phe Asp Phe Asn Gly 
                85                  90                  95      


Val Ser Asn Lys Gly Glu Lys Tyr Ile Thr Lys Val Thr Leu Asn Val 
            100                 105                 110         


Val Ala Pro Ser Leu Glu Val Tyr Val Asp His Ala Ser Leu Pro Thr 
        115                 120                 125             


Leu Gln Gln Leu Met Asp Ile Ile Lys Ser Glu Glu Glu Asn Pro Thr 
    130                 135                 140                 


Ala Gln Arg Tyr Ile Ala Trp Gly Arg Ile Val Pro Thr Asp Glu Gln 
145                 150                 155                 160 


Met Lys Glu Leu Asn Ile Thr Ser Phe Ala Leu Ile Asn Asn His Thr 
                165                 170                 175     


Pro Ala Asp Leu Val Gln Glu Ile Val Lys Gln Ala Gln Thr Lys His 
            180                 185                 190         


Arg Leu Asn Val Lys Leu Ser Ser Asn Thr Ala His Ser Phe Asp Asn 
        195                 200                 205             


Leu Val Pro Ile Leu Lys Glu Leu Asn Ser Phe Asn Asn Val Thr Val 
    210                 215                 220                 


Thr Asn Ile Asp Leu Tyr Asp Asp Gly Ser Ala Glu Tyr Val Asn Leu 
225                 230                 235                 240 


Tyr Asn Trp Arg Asp Thr Leu Asn Lys Thr Asp Asn Leu Lys Ile Gly 
                245                 250                 255     


Lys Asp Tyr Leu Glu Asp Val Ile Asn Gly Ile Asn Glu Asp Thr Ser 
            260                 265                 270         


Asn Thr Gly Thr Ser Ser Val Tyr Asn Trp Gln Lys Leu Tyr Pro Ala 
        275                 280                 285             


Asn Tyr His Phe Leu Arg Lys Asp Tyr Leu Thr Leu Glu Pro Ser Leu 
    290                 295                 300                 


His Glu Leu Arg Asp Tyr Ile Gly Asp Ser Leu Lys Gln Met Gln Trp 
305                 310                 315                 320 


Asp Gly Phe Lys Lys Phe Asn Ser Lys Gln Gln Glu Leu Phe Leu Ser 
                325                 330                 335     


Ile Val Asn Phe Asp Lys Gln Lys Leu Gln Asn Glu Tyr Asn Ser Ser 
            340                 345                 350         


Asn Leu Pro Asn Phe Val Phe Thr Gly Thr Thr Val Trp Ala Gly Asn 
        355                 360                 365             


His Glu Arg Glu Tyr Tyr Ala Lys Gln Gln Ile Asn Val Ile Asn Asn 
    370                 375                 380                 


Ala Ile Asn Glu Ser Ser Pro His Tyr Leu Gly Asn Ser Tyr Asp Leu 
385                 390                 395                 400 


Phe Phe Lys Gly His Pro Gly Gly Gly Ile Ile Asn Thr Leu Ile Met 
                405                 410                 415     


Gln Asn Tyr Pro Ser Met Val Asp Ile Pro Ser Lys Ile Ser Phe Glu 
            420                 425                 430         


Val Leu Met Met Thr Asp Met Leu Pro Asp Ala Val Ala Gly Ile Ala 
        435                 440                 445             


Ser Ser Leu Tyr Phe Thr Ile Pro Ala Glu Lys Ile Lys Phe Ile Val 
    450                 455                 460                 


Phe Thr Ser Thr Glu Thr Ile Thr Asp Arg Glu Thr Ala Leu Arg Ser 
465                 470                 475                 480 


Pro Leu Val Gln Val Met Ile Lys Leu Gly Ile Val Lys Glu Glu Asn 
                485                 490                 495     


Val Leu Phe Trp Ala Asp Leu Pro Asn Cys Glu Thr Gly Val Cys Ile 
            500                 505                 510         


Ala Val 
        


<210>  2
<211>  417
<212>  PRT
<213>  Avibacterium paragallinarum

<400>  2

Met Arg Lys Ile Ile Thr Phe Phe Ser Leu Phe Phe Ser Ile Ser Ala 
1               5                   10                  15      


Trp Cys Gln Lys Met Glu Ile Tyr Leu Asp Tyr Ala Ser Leu Pro Ser 
            20                  25                  30          


Leu Asn Met Ile Leu Asn Leu Val Glu Asn Lys Asn Asn Glu Lys Val 
        35                  40                  45              


Glu Arg Ile Ile Gly Phe Glu Arg Phe Asp Phe Asn Lys Glu Ile Leu 
    50                  55                  60                  


Asn Ser Phe Ser Lys Glu Arg Ile Glu Phe Ser Lys Val Ser Ile Leu 
65                  70                  75                  80  


Asp Ile Lys Glu Phe Ser Asp Lys Leu Tyr Leu Asn Ile Glu Lys Ser 
                85                  90                  95      


Asp Thr Pro Val Asp Leu Ile Ile His Thr Asn Leu Asp His Ser Val 
            100                 105                 110         


Arg Ser Leu Leu Ser Ile Phe Lys Thr Leu Ser Pro Leu Phe His Lys 
        115                 120                 125             


Ile Asn Ile Glu Lys Leu Tyr Leu Tyr Asp Asp Gly Ser Gly Asn Tyr 
    130                 135                 140                 


Val Asp Leu Tyr Gln His Arg Gln Glu Asn Ile Ser Ala Ile Leu Ile 
145                 150                 155                 160 


Glu Ala Gln Lys Lys Leu Lys Asp Ala Leu Glu Asn Arg Glu Thr Asp 
                165                 170                 175     


Thr Asp Lys Leu His Ser Leu Thr Arg Tyr Thr Trp His Lys Ile Phe 
            180                 185                 190         


Pro Thr Glu Tyr Ile Leu Leu Arg Pro Asp Tyr Leu Asp Ile Asp Glu 
        195                 200                 205             


Lys Met Gln Pro Leu Lys His Phe Leu Ser Asp Thr Ile Val Ser Met 
    210                 215                 220                 


Asp Leu Ser Arg Phe Ser His Phe Ser Lys Asn Gln Lys Glu Leu Phe 
225                 230                 235                 240 


Leu Lys Ile Thr His Phe Asp Gln Asn Ile Phe Asn Glu Leu Asn Ile 
                245                 250                 255     


Gly Thr Lys Asn Lys Glu Tyr Lys Thr Phe Ile Phe Thr Gly Thr Thr 
            260                 265                 270         


Thr Trp Glu Lys Asp Lys Lys Lys Arg Leu Asn Asn Ala Lys Leu Gln 
        275                 280                 285             


Thr Glu Ile Leu Glu Ser Phe Ile Lys Pro Asn Gly Lys Phe Tyr Leu 
    290                 295                 300                 


Gly Asn Asp Ile Lys Ile Phe Phe Lys Gly His Pro Lys Gly Asp Asp 
305                 310                 315                 320 


Ile Asn Asp Tyr Ile Ile Arg Lys Thr Gly Ala Glu Lys Ile Pro Ala 
                325                 330                 335     


Asn Ile Pro Phe Glu Val Leu Met Met Thr Asn Ser Leu Pro Asp Tyr 
            340                 345                 350         


Val Gly Gly Ile Met Ser Thr Val Tyr Phe Ser Leu Pro Pro Lys Asn 
        355                 360                 365             


Ile Asp Lys Val Val Phe Leu Gly Ser Glu Lys Ile Lys Asn Glu Asn 
    370                 375                 380                 


Asp Ala Lys Ser Gln Thr Leu Ser Lys Leu Met Leu Met Leu Asn Val 
385                 390                 395                 400 


Ile Thr Pro Glu Gln Ile Phe Phe Glu Glu Met Pro Asn Pro Ile Asn 
                405                 410                 415     


Phe 
    


<210>  3
<211>  264
<212>  PRT
<213>  Actinobacillus ureae

<400>  3

Met Phe Lys Ile Lys Ser Tyr Gly Lys Asn Pro Gln Leu Gln Ala Val 
1               5                   10                  15      


Asp Ile Tyr Ile Asp Phe Ala Thr Ile Pro Ser Leu Ser Tyr Phe Leu 
            20                  25                  30          


His Phe Leu Lys His Lys His Asp His Gln Arg Leu Arg Leu Phe Ser 
        35                  40                  45              


Leu Ala Arg Phe Glu Met Pro Gln Thr Val Ile Glu Gln Tyr Glu Gly 
    50                  55                  60                  


Ile Ile Gln Phe Ser Arg Asn Val Glu His Asn Val Glu Pro Leu Leu 
65                  70                  75                  80  


Glu Gln Leu Gln Thr Ile Leu Ser Gln Glu Gly Lys Gln Phe Glu Leu 
                85                  90                  95      


His Leu His Leu Asn Leu Phe His Ser Phe Glu Met Phe Leu Asn Leu 
            100                 105                 110         


Ser Pro Thr Tyr Thr Lys Tyr Lys Glu Lys Ile Ser Lys Ile Val Leu 
        115                 120                 125             


His Leu Tyr Asp Asp Gly Ser Glu Gly Val Met Lys Gln Tyr Gln Leu 
    130                 135                 140                 


Gln Lys Ser Ser Ser Leu Val Gln Asp Leu Ala Ala Thr Lys Ala Ser 
145                 150                 155                 160 


Leu Val Ser Leu Phe Glu Asn Gly Glu Gly Ser Phe Ser Gln Ile Asp 
                165                 170                 175     


Leu Ile Arg Tyr Val Trp Asn Ala Val Leu Glu Thr His Tyr Tyr Leu 
            180                 185                 190         


Leu Ser Asp His Phe Leu Leu Asp Glu Lys Leu Gln Pro Leu Lys Ala 
        195                 200                 205             


Glu Leu Gly His Tyr Gln Leu Leu Asn Leu Ser Thr Tyr Gln Tyr Leu 
    210                 215                 220                 


Ser Ser Glu Asp Leu Leu Trp Leu Lys Gln Ile Leu Lys Ile Asp Ala 
225                 230                 235                 240 


Glu Leu Glu Ser Leu Met Gln Lys Leu Thr Ala Gln Pro Val Tyr Phe 
                245                 250                 255     


Phe Ser Gly Thr Thr Phe Leu Gly 
            260                 


<210>  4
<211>  399
<212>  PRT
<213>  Haemophilus ducreyi

<400>  4

Met Leu Ile Gln Gln Asn Leu Glu Ile Tyr Leu Asp Tyr Ala Thr Ile 
1               5                   10                  15      


Pro Ser Leu Ala Cys Phe Met His Phe Ile Gln His Lys Asp Asp Val 
            20                  25                  30          


Asp Ser Ile Arg Leu Phe Gly Leu Ala Arg Phe Asp Ile Pro Gln Ser 
        35                  40                  45              


Ile Ile Asp Arg Tyr Pro Ala Asn His Leu Phe Tyr His Asn Ile Asp 
    50                  55                  60                  


Asn Arg Asp Leu Thr Ala Val Leu Asn Gln Leu Ala Asp Ile Leu Ala 
65                  70                  75                  80  


Gln Glu Asn Lys Arg Phe Gln Ile Asn Leu His Leu Asn Leu Phe His 
                85                  90                  95      


Ser Ile Asp Leu Phe Phe Ala Ile Tyr Pro Ile Tyr Gln Gln Tyr Gln 
            100                 105                 110         


His Lys Ile Ser Thr Ile Gln Leu Gln Leu Tyr Asp Asp Gly Ser Glu 
        115                 120                 125             


Gly Ile Val Thr Gln His Ser Leu Cys Lys Ile Ala Asp Leu Glu Gln 
    130                 135                 140                 


Leu Ile Leu Gln His Lys Asn Val Leu Leu Glu Leu Leu Thr Lys Gly 
145                 150                 155                 160 


Thr Ala Asn Val Pro Asn Pro Thr Leu Leu Arg Tyr Leu Trp Asn Asn 
                165                 170                 175     


Ile Ile Asp Ser Gln Phe His Leu Ile Ser Asp His Phe Leu Gln His 
            180                 185                 190         


Pro Lys Leu Gln Pro Leu Lys Arg Leu Leu Lys Arg Tyr Thr Ile Leu 
        195                 200                 205             


Asp Phe Thr Cys Tyr Pro Arg Phe Asn Ala Glu Gln Lys Gln Leu Leu 
    210                 215                 220                 


Lys Glu Ile Leu His Ile Ser Asn Glu Leu Glu Asn Leu Leu Lys Leu 
225                 230                 235                 240 


Leu Lys Gln His Asn Thr Phe Leu Phe Thr Gly Thr Thr Ala Phe Asn 
                245                 250                 255     


Leu Asp Gln Glu Lys Leu Asp Leu Leu Thr Gln Leu His Ile Leu Leu 
            260                 265                 270         


Leu Asn Glu His Gln Asn Pro His Ser Thr His Tyr Ile Gly Asn Asn 
        275                 280                 285             


Tyr Leu Leu Leu Ile Lys Gly His Ala Asn Ser Pro Ala Leu Asn His 
    290                 295                 300                 


Thr Leu Ala Leu His Phe Pro Asp Ala Ile Phe Leu Pro Ala Asn Ile 
305                 310                 315                 320 


Pro Phe Glu Ile Phe Ala Met Leu Gly Phe Thr Pro Asn Lys Met Gly 
                325                 330                 335     


Gly Phe Ala Ser Thr Ser Tyr Ile Asn Tyr Pro Thr Glu Asn Ile Asn 
            340                 345                 350         


His Leu Phe Phe Leu Thr Ser Asp Gln Pro Ser Ile Arg Thr Lys Trp 
        355                 360                 365             


Leu Asp Tyr Glu Lys Gln Phe Gly Leu Met Tyr Ser Leu Leu Ala Met 
    370                 375                 380                 


Gln Lys Ile Asn Glu Asp Gln Ala Phe Met Cys Thr Ile His Asn 
385                 390                 395                 


<210>  5
<211>  498
<212>  PRT
<213>  Alistipes (multi species)

<400>  5

Met Lys Arg Leu Phe Arg Leu Phe Leu Cys Leu Ala Leu Leu Ser Gly 
1               5                   10                  15      


Thr Ala Ala Cys Ser Asp Asp Glu Val Ser Gln Asn Leu Ile Val Ile 
            20                  25                  30          


Asn Gly Gly Glu His Phe Leu Ser Leu Asp Gly Leu Ala Arg Ala Gly 
        35                  40                  45              


Lys Ile Ser Val Leu Ala Pro Ala Pro Trp Arg Val Thr Lys Ala Ala 
    50                  55                  60                  


Gly Asp Thr Trp Phe Arg Leu Ser Ala Thr Glu Gly Pro Ala Gly Tyr 
65                  70                  75                  80  


Ser Glu Val Glu Leu Ser Leu Asp Glu Asn Pro Gly Ala Ala Arg Ser 
                85                  90                  95      


Ala Gln Leu Ala Phe Ala Cys Gly Asp Ala Ile Val Pro Phe Arg Leu 
            100                 105                 110         


Ser Gln Gly Ala Leu Ser Ala Gly Tyr Asp Ser Pro Asp Tyr Tyr Phe 
        115                 120                 125             


Tyr Val Thr Phe Gly Thr Met Pro Thr Leu Tyr Ala Gly Ile His Leu 
    130                 135                 140                 


Leu Ser His Asp Lys Pro Gly Tyr Val Phe Tyr Ser Arg Ser Lys Thr 
145                 150                 155                 160 


Phe Asp Pro Ala Glu Phe Pro Ala Arg Ala Glu Val Thr Thr Ala Ala 
                165                 170                 175     


Asp Arg Thr Ala Asp Ala Thr Gln Ala Glu Met Glu Ala Met Ala Arg 
            180                 185                 190         


Glu Met Lys Arg Arg Ile Leu Glu Ile Asn Ser Ala Asp Pro Thr Ala 
        195                 200                 205             


Val Phe Gly Leu Tyr Val Asp Asp Leu Arg Cys Arg Ile Gly Tyr Asp 
    210                 215                 220                 


Trp Phe Val Ala Gln Gly Ile Asp Ser Ala Arg Val Lys Val Ser Met 
225                 230                 235                 240 


Leu Ser Asp Gly Thr Gly Thr Tyr Asn Asn Phe Tyr Asn Tyr Phe Gly 
                245                 250                 255     


Asp Ala Ala Thr Ala Glu Gln Asn Trp Glu Ser Tyr Ala Ser Glu Val 
            260                 265                 270         


Glu Ala Leu Asp Trp Asn His Gly Gly Arg Tyr Pro Glu Thr Arg Ser 
        275                 280                 285             


Leu Pro Glu Phe Glu Ser Tyr Thr Trp Pro Tyr Tyr Leu Ser Thr Arg 
    290                 295                 300                 


Pro Asp Tyr Arg Leu Val Val Gln Asp Gly Ser Leu Leu Glu Ser Ser 
305                 310                 315                 320 


Cys Pro Phe Ile Thr Glu Lys Leu Gly Glu Met Glu Ile Glu Ser Ile 
                325                 330                 335     


Gln Pro Tyr Glu Met Leu Ser Ala Leu Pro Glu Ser Ser Arg Lys Arg 
            340                 345                 350         


Phe Tyr Asp Met Ala Gly Phe Asp Tyr Asp Lys Phe Ala Ala Leu Phe 
        355                 360                 365             


Asp Ala Ser Pro Lys Lys Asn Leu Ile Ile Ile Gly Thr Ser His Ala 
    370                 375                 380                 


Asp Asp Ala Ser Ala Arg Leu Gln Arg Asp Tyr Val Ala Arg Ile Met 
385                 390                 395                 400 


Glu Gln Tyr Gly Ala Gln Tyr Asp Val Phe Phe Lys Pro His Pro Ala 
                405                 410                 415     


Asp Thr Thr Ser Ala Gly Tyr Glu Thr Glu Phe Pro Gly Leu Thr Leu 
            420                 425                 430         


Leu Pro Gly Gln Met Pro Phe Glu Ile Phe Val Trp Ser Leu Ile Asp 
        435                 440                 445             


Arg Val Asp Met Ile Gly Gly Tyr Pro Ser Thr Val Phe Leu Thr Val 
    450                 455                 460                 


Pro Val Asp Lys Val Arg Phe Ile Phe Ala Ala Asp Ala Ala Ser Leu 
465                 470                 475                 480 


Val Arg Pro Leu Asn Ile Leu Phe Arg Asp Ala Thr Asp Val Glu Trp 
                485                 490                 495     


Met Gln 
        


<210>  6
<211>  410
<212>  PRT
<213>  Bibersteinia trealosi

<400>  6

Met Glu Phe Cys Lys Met Ala Thr Thr Gln Lys Ile Cys Val Tyr Leu 
1               5                   10                  15      


Asp Tyr Ala Thr Ile Pro Ser Leu Asn Tyr Ile Leu His Phe Ala Gln 
            20                  25                  30          


His Phe Glu Asp Gln Glu Thr Ile Arg Leu Phe Gly Leu Ser Arg Phe 
        35                  40                  45              


His Ile Pro Glu Ser Val Ile Gln Arg Tyr Pro Lys Gly Val Val Gln 
    50                  55                  60                  


Phe Tyr Pro Asn Gln Glu Lys Asp Phe Ser Ala Leu Leu Leu Ala Leu 
65                  70                  75                  80  


Lys Asn Ile Leu Ile Glu Val Lys Gln Gln Gln Arg Lys Cys Glu Ile 
                85                  90                  95      


Glu Leu His Leu Asn Leu Phe His Tyr Gln Leu Leu Leu Leu Pro Phe 
            100                 105                 110         


Leu Ser Leu Tyr Leu Asp Thr Gln Asp Tyr Cys His Leu Thr Leu Lys 
        115                 120                 125             


Phe Tyr Asp Asp Gly Ser Glu Ala Ile Ser Ala Leu Gln Glu Leu Ala 
    130                 135                 140                 


Leu Ala Pro Asp Leu Ala Ala Gln Ile Gln Phe Glu Lys Gln Gln Phe 
145                 150                 155                 160 


Asp Glu Leu Val Val Lys Lys Ser Phe Lys Leu Ser Leu Leu Ser Arg 
                165                 170                 175     


Tyr Phe Trp Gly Lys Leu Phe Glu Ser Glu Tyr Ile Trp Phe Asn Gln 
            180                 185                 190         


Ala Ile Leu Gln Lys Ala Glu Leu Gln Ile Leu Lys Gln Glu Ile Ser 
        195                 200                 205             


Ser Ser Arg Gln Met Asp Phe Ala Ile Tyr Gln Gln Met Ser Asp Glu 
    210                 215                 220                 


Gln Lys Gln Leu Val Leu Glu Ile Leu Asn Ile Asp Leu Asn Lys Val 
225                 230                 235                 240 


Ala Tyr Leu Lys Gln Leu Met Glu Asn Gln Pro Ser Phe Leu Phe Leu 
                245                 250                 255     


Gly Thr Thr Leu Phe Asn Ile Thr Gln Glu Thr Lys Thr Trp Leu Met 
            260                 265                 270         


Gln Met His Val Asp Leu Ile Gln Gln Tyr Cys Leu Pro Ser Gly Gln 
        275                 280                 285             


Phe Phe Asn Asn Lys Ala Gly Tyr Leu Cys Phe Tyr Lys Gly His Pro 
    290                 295                 300                 


Asn Glu Lys Glu Met Asn Gln Met Ile Leu Ser Gln Phe Lys Asn Leu 
305                 310                 315                 320 


Ile Ala Leu Pro Asp Asp Ile Pro Leu Glu Ile Leu Leu Leu Leu Gly 
                325                 330                 335     


Val Ile Pro Ser Lys Val Gly Gly Phe Ala Ser Ser Ala Leu Phe Asn 
            340                 345                 350         


Phe Thr Pro Ala Gln Ile Glu Asn Ile Ile Phe Phe Thr Pro Arg Tyr 
        355                 360                 365             


Phe Glu Lys Asp Asn Arg Leu His Ala Thr Gln Tyr Arg Leu Met Gln 
    370                 375                 380                 


Gly Leu Ile Glu Leu Gly Tyr Leu Asp Ala Glu Lys Ser Val Thr His 
385                 390                 395                 400 


Phe Glu Ile Met Gln Leu Leu Thr Lys Glu 
                405                 410 


<210>  7
<211>  367
<212>  PRT
<213>  Shewanella piezotolerans

<400>  7

Met Leu Val Asn Asn Gln Ser His Asn Pro Lys Leu Ile Cys Trp Gln 
1               5                   10                  15      


Arg His Pro Val Asn Asp Glu Ala Leu Leu Gln Gly Ile Asn Ala Ala 
            20                  25                  30          


Ser Phe Val Ser Ile Ala Ser Leu Cys Gln His Ala Ala Thr Leu Leu 
        35                  40                  45              


Ala Gly His Pro His Ser His Ile Thr Ile Tyr Gly Asn Thr Tyr Trp 
    50                  55                  60                  


Ser Lys Asp Leu Ala Arg Leu Ile Arg Tyr Leu Thr Arg Ile Ser Gly 
65                  70                  75                  80  


Val Glu Ile Lys Lys Leu Glu Leu Ile Asp Asp Gly Ser Ser Glu Tyr 
                85                  90                  95      


Gln Lys Met Phe Tyr Trp Gln Arg Leu Ser Ser Glu Glu Gln Thr Arg 
            100                 105                 110         


Asp Leu Ala Thr Gly Leu Lys Asn Leu Lys Ser Tyr Leu Ser Gly Asn 
        115                 120                 125             


Asp Asn Lys Leu Leu Arg Leu Leu Thr Gly His Ser Asn Lys Leu Pro 
    130                 135                 140                 


Arg Arg Leu Ser Ser Phe Met Asn Trp His Gln Leu Phe Pro Thr Thr 
145                 150                 155                 160 


Tyr His Met Leu Arg Met Asp Tyr Leu Asp Lys Pro Glu Leu His Gln 
                165                 170                 175     


Leu Lys Gln Tyr Leu Gly Asn Asn Ala Gln Gln Ile Arg Trp Asn Tyr 
            180                 185                 190         


Ile Ala Asp Asn Leu Phe Asp Asp Glu Gln Gln Ser Leu Phe Tyr Gln 
        195                 200                 205             


Leu Leu Gly Ile Ser Leu Ala Glu Gln Lys Gln Leu Arg Ala Gly Arg 
    210                 215                 220                 


Gln Gln Leu His Asp Phe Met Phe Ile Gly Val Asp Ser Ser Asn Ala 
225                 230                 235                 240 


Ser Ser Lys Leu Gln Ile Asn Val Ile Ala Asp Ser Arg Gln Glu Ser 
                245                 250                 255     


Gly Ile Ile Pro Thr Ile Thr Ala Lys Lys Met Leu Phe Lys Gly His 
            260                 265                 270         


Pro Phe Ala Asn Phe Asn Gln Thr Ile Val Asp Ala His Gln Met Gly 
        275                 280                 285             


Glu Met Pro Ala Met Ile Pro Phe Glu Thr Leu Ile Met Thr Gly Asn 
    290                 295                 300                 


Leu Pro Gln Lys Val Gly Gly Met Ala Ser Ser Leu Tyr Phe Ser Leu 
305                 310                 315                 320 


Pro Asn Asn Tyr His Ile Glu Tyr Ile Val Phe Ser Gly Ser Lys Lys 
                325                 330                 335     


Asp Leu Glu Gln His Ala Leu Leu Gln Ile Met Leu Tyr Leu Lys Val 
            340                 345                 350         


Ile Ser Pro Glu Arg Val Tyr Phe Ser Glu Gln Phe Lys Ser Cys 
        355                 360                 365         


<210>  8
<211>  422
<212>  PRT
<213>  Helicobacter acinonychis

<400>  8

Met Gly Thr Ile Lys Lys Pro Leu Ile Ile Ala Gly Asn Gly Pro Ser 
1               5                   10                  15      


Ile Lys Asp Leu Asp Tyr Ala Leu Phe Pro Lys Asp Phe Asp Val Phe 
            20                  25                  30          


Arg Cys Asn Gln Phe Tyr Phe Glu Asp Lys Tyr Tyr Leu Gly Arg Glu 
        35                  40                  45              


Ile Lys Gly Val Phe Phe Asn Pro Cys Val Leu Ser Ser Gln Met Gln 
    50                  55                  60                  


Thr Val Gln Tyr Leu Met Asp Asn Gly Glu Tyr Ser Ile Glu Arg Phe 
65                  70                  75                  80  


Phe Cys Ser Val Ser Thr Asp Arg His Asp Phe Asp Gly Asp Tyr Gln 
                85                  90                  95      


Thr Ile Leu Pro Val Asp Gly Tyr Leu Lys Ala His Tyr Pro Phe Val 
            100                 105                 110         


Cys Asp Thr Phe Ser Leu Phe Lys Gly His Glu Glu Ile Leu Lys His 
        115                 120                 125             


Val Lys Tyr His Leu Lys Thr Tyr Ser Lys Glu Leu Ser Ala Gly Val 
    130                 135                 140                 


Leu Met Leu Leu Ser Ala Val Val Leu Gly Tyr Lys Glu Ile Tyr Leu 
145                 150                 155                 160 


Val Gly Ile Asp Phe Gly Ala Ser Ser Trp Gly His Phe Tyr Asp Glu 
                165                 170                 175     


Ser Gln Ser Gln His Phe Ser Asn His Met Ala Asp Cys His Asn Ile 
            180                 185                 190         


Tyr Tyr Asp Met Leu Thr Ile Cys Leu Cys Gln Lys Tyr Ala Lys Leu 
        195                 200                 205             


Tyr Ala Leu Ala Pro Asn Ser Pro Leu Ser His Leu Leu Thr Leu Asn 
    210                 215                 220                 


Pro Gln Ala Lys Tyr Pro Phe Glu Leu Leu Asp Lys Pro Ile Gly Tyr 
225                 230                 235                 240 


Thr Ser Asp Leu Ile Ile Ser Ser Pro Leu Glu Glu Lys Leu Leu Glu 
                245                 250                 255     


Phe Lys Asn Ile Glu Glu Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu 
            260                 265                 270         


Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu Lys Leu Leu Glu Phe Lys 
        275                 280                 285             


Asn Ile Glu Glu Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu Lys Leu 
    290                 295                 300                 


Leu Glu Phe Lys Asn Ile Glu Glu Lys Leu Leu Glu Phe Lys Asn Ile 
305                 310                 315                 320 


Glu Glu Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu Lys Leu Leu Glu 
                325                 330                 335     


Phe Lys Asn Ile Glu Glu Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu 
            340                 345                 350         


Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu Lys Leu Leu Glu Phe Lys 
        355                 360                 365             


Asn Ile Glu Glu Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu Lys Leu 
    370                 375                 380                 


Leu Ala Ser Arg Leu Asn Asn Ile Leu Arg Lys Ile Lys Arg Lys Ile 
385                 390                 395                 400 


Leu Pro Phe Phe Trp Gly Gly Gly Val Thr Pro Thr Leu Lys Val Ser 
                405                 410                 415     


Phe Arg Trp Gly Ala Ala 
            420         


<210>  9
<211>  318
<212>  PRT
<213>  Helicobacter pylori

<400>  9

Met Lys Lys Pro Leu Ile Ile Ala Gly Asn Gly Pro Ser Ile Lys Asp 
1               5                   10                  15      


Leu Asp Tyr Ser Leu Phe Pro Lys Asp Phe Glu Val Phe Arg Cys Asn 
            20                  25                  30          


Gln Phe Tyr Phe Glu Asp Lys Tyr Tyr Leu Gly Arg Glu Ile Lys Gly 
        35                  40                  45              


Val Phe Phe Asn Pro Cys Val Leu Ser Ser Gln Met Gln Thr Ala Gln 
    50                  55                  60                  


Tyr Leu Met Asp Asn Gly Glu Tyr Ser Ile Glu Arg Phe Phe Cys Ser 
65                  70                  75                  80  


Val Ser Thr Asp Arg His Asp Phe Asp Gly Asp Tyr Gln Thr Ile Leu 
                85                  90                  95      


Pro Val Glu Gly Tyr Leu Lys Ala His Tyr Pro Phe Val Cys Asp Thr 
            100                 105                 110         


Phe Ser Leu Phe Lys Gly His Glu Glu Ile Leu Arg His Val Lys Tyr 
        115                 120                 125             


His Leu Lys Thr Tyr Ser Lys Glu Leu Ser Ala Gly Val Leu Met Leu 
    130                 135                 140                 


Leu Ser Ala Val Val Leu Gly Tyr Lys Glu Ile Tyr Leu Val Gly Ile 
145                 150                 155                 160 


Asp Phe Gly Ala Ser Ser Trp Gly His Phe Tyr Asp Glu Ser Gln Ser 
                165                 170                 175     


Gln His Phe Ser Asn His Met Ala Asp Cys His Asn Ile Tyr Tyr Asp 
            180                 185                 190         


Met Phe Thr Ile Cys Leu Cys Gln Lys Tyr Ala Lys Leu Tyr Ala Leu 
        195                 200                 205             


Ala Pro Asn Ser Pro Leu Arg His Ile Leu Ala Leu Asn Pro Gln Ala 
    210                 215                 220                 


Lys Tyr His Phe Glu Leu Leu Asp Lys Pro Ile Gly Tyr Thr Ser Asp 
225                 230                 235                 240 


Leu Ile Val Ser Leu Pro Leu Glu Glu Lys Leu Leu Glu Phe Lys Asn 
                245                 250                 255     


Ile Glu Glu Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu Lys Leu Leu 
            260                 265                 270         


Glu Phe Lys Asn Ile Glu Glu Lys Leu Leu Val Asn Arg Leu Lys Asn 
        275                 280                 285             


Ile Leu Arg Lys Ile Lys Arg Lys Ile Leu Pro Phe Trp Gly Gly Gly 
    290                 295                 300                 


Gly Asn Thr His Leu Lys Val Ser Phe Arg Trp Gly Val Ala 
305                 310                 315             


<210>  10
<211>  314
<212>  PRT
<213>  Helicobacter cetorum

<400>  10

Met Ser Glu Lys Ile Phe Ser Gln Val Asp Glu Lys Asn Gln Lys Lys 
1               5                   10                  15      


Pro Leu Ile Ile Ala Gly Asn Gly Pro Ser Ile Lys Asp Leu Asp Tyr 
            20                  25                  30          


Ser Leu Phe Pro Lys Asp Phe Asp Val Phe Arg Cys Asn Gln Phe Tyr 
        35                  40                  45              


Phe Glu Asp Lys Tyr Tyr Leu Gly Lys Glu Val Lys Gly Val Phe Phe 
    50                  55                  60                  


Asn Pro Cys Val Phe His Asn Gln Met Asn Thr Ala Lys His Leu Ile 
65                  70                  75                  80  


Asp Asn Asn Glu Tyr Tyr Ile Glu Gln Phe Phe Cys Ser Val Ser Lys 
                85                  90                  95      


Glu Gln His Asp Phe Asn Gly Asp Tyr Gln Thr Ile Leu Ser Val Asp 
            100                 105                 110         


Glu Tyr Leu Arg Ala Asn Tyr Pro Phe Val Arg Asp Thr Phe Ser Leu 
        115                 120                 125             


Phe Gly Glu His Glu Glu Ile Leu Asn His Val Lys Tyr His Leu Lys 
    130                 135                 140                 


Thr Tyr Ser Lys Glu Leu Ser Ala Gly Val Leu Met Leu Leu Ser Ala 
145                 150                 155                 160 


Ile Val Leu Gly Tyr Lys Glu Ile Tyr Leu Val Gly Val Asp Phe Gly 
                165                 170                 175     


Ala Asn Ser Trp Gly His Phe Tyr Asp Asp Asn Gln Ser Gln His Phe 
            180                 185                 190         


Ile Asn His Met Ala Asp Cys His Asn Ile Tyr Tyr Asp Met Leu Thr 
        195                 200                 205             


Ile Tyr Leu Cys Gln Lys Tyr Ala Lys Leu Tyr Ala Leu Val Pro Asn 
    210                 215                 220                 


Ser Pro Leu Asn His Leu Leu Pro Leu Asn Leu Gln Ala Asn His Val 
225                 230                 235                 240 


Phe Glu Leu Leu Asp Lys Pro Ile Gly Tyr Thr Ser Asp Leu Ile Val 
                245                 250                 255     


Ser Ser Pro Leu Glu Glu Lys Leu Leu Glu Ser Lys Asn Ile Asp Glu 
            260                 265                 270         


Arg Phe Ser Gln Asn Lys Ser Phe Lys Asn Tyr Leu Gln Arg Leu Lys 
        275                 280                 285             


Asp Lys Phe Leu Gln Met Ile Phe Arg Gly Gly Gly Val Ile Thr Ile 
    290                 295                 300                 


Pro Arg Val Ile Phe Lys Gly Lys Phe Ala 
305                 310                 


<210>  11
<211>  8184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  11
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg      240

cctcctcaac ctgtatattc gtaaaccacg cccaatggga gctgtctcag gtttgttcct      300

gattggttac ggcgcgtttc gcatcattgt tgagtttttc cgccagcccg acgcgcagtt      360

taccggtgcc tgggtgcagt acatcagcat ggggcaaatt ctttccatcc cgatgattgt      420

cgcgggtgtg atcatgatgg tctgggcata tcgtcgcagc ccacagcaac acgtttcctg      480

aggaaccatg aaacagtatt tagaactgat gcaaaaagtg ctcgacgaag gcacacagaa      540

aaacgaccgt accggaaccg gaacgctttc catttttggt catcagatgc gttttaacct      600

gcaagatgga ttcccgctgg tgacaactaa acgttgccac ctgcgttcca tcatccatga      660

actgctgtgg tttctgcagg gcgacactaa cattgcttat ctacacgaaa acaatgtcac      720

catctgggac gaatgggccg atgaaaacgg cgacctcggg ccagtgtatg gtaaacagtg      780

gcgcgcctgg ccaacgccag atggtcgtca tattgaccag atcactacgg tactgaacca      840

gctgaaaaac gacccggatt cgcgccgcat tattgtttca gcgtggaacg taggcgaact      900

ggataaaatg gcgctggcac cgtgccatgc attcttccag ttctatgtgg cagacggcaa      960

actctcttgc cagctttatc agcgctcctg tgacgtcttc ctcggcctgc cgttcaacat     1020

tgccagctac gcgttattgg tgcatatgat ggcgcagcag tgcgatctgg aagtgggtga     1080

ttttgtctgg accggtggcg acacgcatct gtacagcaac catatggatc aaactcatct     1140

gcaattaagc cgcgaaccgc gtccgctgcc gaagttgatt atcaaacgta aacccgaatc     1200

catcttcgac taccgtttcg aagactttga gattgaaggc tacgatccgc atccgggcat     1260

taaagcgccg gtggctatct aattacgaaa catcctgcca gagccgacgc cagtgtgcgt     1320

cggttttttt accctccgtt aaattcttcg agacgccttc ccgaaggcgc cattcgccat     1380

tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc     1440

tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt     1500

cacgacgttg taaaacgacg gccagtgcca agcttactgc tcacaagaaa aaaggcacgt     1560

catctgacgt gcctttttta tttgtactac cctgtacgat tactgcaggt cgacttattt     1620

tttccatatc tgttcaacct tttttaaatc ctccaaacag tcaatatcta aacttgagct     1680

ttcgtccatt aaaaaatgct tggttttgct ttgtaaaaag ctaggattgt ttaaaaattc     1740

ttttatcttt aaaatataaa ttgcaccatt gctcatataa gttttaggca atttttgcct     1800

tggcataaaa ggatattcat cattacaaat ccctgctaaa tcgccacaat cattacaaac     1860

aaaggctttt agaattttat tatcacattc gcttacgcta attagggcat ttgcattgct     1920

atttttataa agattaaaag cttcattaat atgaatattt gttcttagcg gtgaagtggg     1980

ttgtaaaaaa actacatctt cataatcttt ataaaatttt agagcatgta acagcacttt     2040

atcgcttgtg gtatcatctt gtgcaaggct aattgggcgt tttaaaatat caacattttg     2100

actttttgca taatttaaaa tttcatcact atcactgctt acaacaactt tactaatgct     2160

tttagcattt agtgcagctt tgatcgtgta gtaaattaaa ggtttattgt ttaataaaac     2220

caaattttta tttttaatac cctttgagcc accacgagca gggattattg ctaagctcat     2280

tttatatcct taaaaacttt ttgtgtgctg agtttaaaaa aatctccgct ttgtaaatat     2340

tcaaaaaata attttgagct atctaaaatc tctaacttag cgctaaataa atcttgtttt     2400

ttatgaatag tgttaatagc ttttagtatt tcatcactat ttgcattaac ttttagtgta     2460

ttttcattgc caagtcttcc attttgtctt gagccaacta aaatccctgc tgtttttaag     2520

tataaggcct cttttaaaat acaacttgaa ttacctatta taaaatcagc attttttaac     2580

aaagttataa aatactcaaa tctaagcgat ggaaaaagct taaatctagg gttattttta     2640

aactcttcat agctttgcaa gattaattca aaacctaaat cattatttgg ataaataaca     2700

atataatttt tattactttg tatcagtgct tttactaaat tgtctgcttg atttttaatg     2760

ctagtaattt cagttgtaac aggatgaaac ataagcaaag cgtagttttc ataatttata     2820

tcataatatt tttttgcttc gctaagtgaa attttattat cgtttaaaag ttctaaatca     2880

ggcgaaccta tgataaaaat agatttttca tcttctccaa gctgcattaa acgccttttt     2940

gcaaactcat catttactaa atgaatatga gctagttttg atatagcgtg gcgtaagcta     3000

tcgtcaatag ttcctgaaat ctctccgcct tcaatatgcg ctactaagat attatttaat     3060

gctccaacaa tagctgctgc taaaggctca attctatctc catgtactac gattaaatca     3120

ggttttagct catttgcata ccttgaaaat ccatcaattg tagtagctaa agccttatca     3180

gtttgataat atttatcata atttataaat tcataaatat ttttaaagcc atttttataa     3240

agttctttaa ctgtatagcc aaaattttta cttaagtgca ttcctgttgc aaagatgtaa     3300

agttcaaatt cgcttgagtt ttgcaccctg tacattaaag atttaatctt agaataatca     3360

gccctagagc ctgttataaa aaggattttt ttcacgcaaa atcctcatag cttaactgag     3420

catcattttc tatatctctt aatgcttttt tgcctaaaat attttcaaat tcagccgcac     3480

taattccacc aagtccaggt cttttaaccc aaatattatc catagataaa acttcgcctt     3540

ttttaatatc tttaatgcta actacacttg caaaggcaaa atcaattgta acttgttctt     3600

gtttagccgc ttttttactt tcattatttc ctcttattat agccatttgc tcactttgta     3660

taattagctc ttttaaagcc tttgtatcca tagaacaaac tatatcaggg ccacttctat     3720

gcatactatc agtaaaatgt ctttcaagca cacaagctcc aagtacaact gcacctaaac     3780

acgcaagatt atctgttgtg tggtcgctta agcctaccat acaagaaaat tcttttttta     3840

actcaagcat agcgtttaat cttacaagat tatgcggggt tgggtaaaga ttggtcgtgt     3900

gcattaaaac aaaaggaatt tcattgtcta ataagatttt tacagttggt tttatacttt     3960

caatactatt cattcctgtg ctaactatca taggcttttt aaaggctgct atgtgtttaa     4020

taagcggata attattacac tcacctgaac caatcttaaa agcactaact cccatatctt     4080

ctaagcggtt cgcacctgca cgagaaaaag gtgtgctaag ataaacaaga cctaattttt     4140

ctgtgtattc tttaagtgct agctcatctt tataatccaa agcacatttt tgcataatct     4200

cataaatgct tatttttgca ttaccaggaa ttactttttt agcggcctta ctcatctcat     4260

cttcaacaat atgagtttga tgctttataa tcttagcacc tgcgctaaag gctgcatcta     4320

ccataatttt agctagttct aaactgccat tatgattaat gcctatttca ggtacgacta     4380

agggtgcttt ttcttcactt atgattatat tttgtatttt tatttctttc atttattttc     4440

ctccttagtc gacggtaccc ttaagcgaat tttcctttaa agatcacgcg gggaattgta     4500

atgactccac ccccacggaa gatcatttga agaaacttat ctttaagacg ttgaagatag     4560

tttttgaagg acttattctg agagaagcgc tcgtcgatgt tcttcgactc taacagtttt     4620

tcttctaaag gggagctaac gattaaatcc gacgtgtagc cgatgggctt atcaagcagc     4680

tcaaatacat ggtttgcctg taagttcaac ggtaaaagat ggttcagagg actgttaggt     4740

actaaagcat ataatttggc gtatttttga caaaggtaaa tagtcaacat gtcataataa     4800

atgttatggc agtcagccat gtggttaata aagtgctgac tctggttgtc atcgtaaaaa     4860

tgtccccagc tatttgcgcc aaaatcgaca ccgactaagt agatttcctt gtatcctaaa     4920

acaattgcgc tcaacaacat aaggaccccc gcagataatt cttttgaata tgtcttcaga     4980

tggtatttga catggtttaa gatttcctca tgctccccaa acaagctaaa ggtgtcacgt     5040

acaaacgggt agtttgcacg aaggtattcg tccaccgata agatggtctg gtaatcaccg     5100

ttaaaatcgt gttgttcttt cgacacacta caaaagaact gctcgatgta gtattcgttg     5160

ttgtcaatta aatgcttcgc ggtattcatt tgattatgga agacgcacgg attaaagaat     5220

acacctttga cctctttgcc caagtaatac ttatcttcga aatagaattg gttacagcgg     5280

aaaacgtcga aatcttttgg gaacaacgaa tagtcaaggt ctttgattga tggtccgttg     5340

cccgcgataa tcaagggctt tttttggttc ttctcgtcaa cctggctgaa gattttttcc     5400

gacatatgta tatctccttc ttgaattcta acaattgatt gaatgtatgc aaataaatgc     5460

atacaccata ggtgtggttt aatttgatgc cctttttcag ggctggaatg tgtaagagcg     5520

gggttattta tgctgttgtt tttttgttac tcgggaaggg ctttacctct tccgcataaa     5580

cgcttccatc agcgtttata gttaaaaaaa tctttcggaa ctggttttgc gcttacccca     5640

accaacaggg gatttgctgc tttccattga gcctgtttct ctgcgcgacg ttcgcggcgg     5700

cgtgtttgtg catccatctg gattctcctg tcagttagct ttggtggtgt gtggcagttg     5760

tagtcctgaa cgaaaacccc ccgcgattgg cacattggca gctaatccgg aatcgcactt     5820

acggccaatg cttcgtttcg tatcacacac cccaaagcct tctgctttga atgctgccct     5880

tcttcagggc ttaattttta agagcgtcac cttcatggtg gtcagtgcgt cctgctgatg     5940

tgctcagtat caccgccagt ggtatttatg tcaacaccgc cagagataat ttatcaccgc     6000

agatggttat ctgtatgttt tttatatgaa tttatttttt gcaggggggc attgtttggt     6060

aggtgagaga tcaattctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     6120

tattgggcgc tcttccgctg ctagcggagt gtatactggc ttactatgtt ggcactgatg     6180

agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca     6240

gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt     6300

tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag     6360

gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc     6420

ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga     6480

ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc     6540

tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac     6600

actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca     6660

gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc     6720

aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat     6780

gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt     6840

tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg     6900

ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc     6960

ttattaatca gataaaatat ttctaggcgg ccgcgaacga aaactcacgt taagggattt     7020

tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt     7080

ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca     7140

gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg     7200

tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac     7260

cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg     7320

ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc     7380

gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta     7440

caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac     7500

gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc     7560

ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac     7620

tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact     7680

caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa     7740

tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt     7800

cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca     7860

ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa     7920

aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac     7980

tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg     8040

gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc     8100

gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata     8160

ggcgtatcac gaggcccttt cgtc                                            8184


<210>  12
<211>  8196
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  12
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg      240

cctcctcaac ctgtatattc gtaaaccacg cccaatggga gctgtctcag gtttgttcct      300

gattggttac ggcgcgtttc gcatcattgt tgagtttttc cgccagcccg acgcgcagtt      360

taccggtgcc tgggtgcagt acatcagcat ggggcaaatt ctttccatcc cgatgattgt      420

cgcgggtgtg atcatgatgg tctgggcata tcgtcgcagc ccacagcaac acgtttcctg      480

aggaaccatg aaacagtatt tagaactgat gcaaaaagtg ctcgacgaag gcacacagaa      540

aaacgaccgt accggaaccg gaacgctttc catttttggt catcagatgc gttttaacct      600

gcaagatgga ttcccgctgg tgacaactaa acgttgccac ctgcgttcca tcatccatga      660

actgctgtgg tttctgcagg gcgacactaa cattgcttat ctacacgaaa acaatgtcac      720

catctgggac gaatgggccg atgaaaacgg cgacctcggg ccagtgtatg gtaaacagtg      780

gcgcgcctgg ccaacgccag atggtcgtca tattgaccag atcactacgg tactgaacca      840

gctgaaaaac gacccggatt cgcgccgcat tattgtttca gcgtggaacg taggcgaact      900

ggataaaatg gcgctggcac cgtgccatgc attcttccag ttctatgtgg cagacggcaa      960

actctcttgc cagctttatc agcgctcctg tgacgtcttc ctcggcctgc cgttcaacat     1020

tgccagctac gcgttattgg tgcatatgat ggcgcagcag tgcgatctgg aagtgggtga     1080

ttttgtctgg accggtggcg acacgcatct gtacagcaac catatggatc aaactcatct     1140

gcaattaagc cgcgaaccgc gtccgctgcc gaagttgatt atcaaacgta aacccgaatc     1200

catcttcgac taccgtttcg aagactttga gattgaaggc tacgatccgc atccgggcat     1260

taaagcgccg gtggctatct aattacgaaa catcctgcca gagccgacgc cagtgtgcgt     1320

cggttttttt accctccgtt aaattcttcg agacgccttc ccgaaggcgc cattcgccat     1380

tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc     1440

tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt     1500

cacgacgttg taaaacgacg gccagtgcca agcttactgc tcacaagaaa aaaggcacgt     1560

catctgacgt gcctttttta tttgtactac cctgtacgat tactgcaggt cgacttattt     1620

tttccatatc tgttcaacct tttttaaatc ctccaaacag tcaatatcta aacttgagct     1680

ttcgtccatt aaaaaatgct tggttttgct ttgtaaaaag ctaggattgt ttaaaaattc     1740

ttttatcttt aaaatataaa ttgcaccatt gctcatataa gttttaggca atttttgcct     1800

tggcataaaa ggatattcat cattacaaat ccctgctaaa tcgccacaat cattacaaac     1860

aaaggctttt agaattttat tatcacattc gcttacgcta attagggcat ttgcattgct     1920

atttttataa agattaaaag cttcattaat atgaatattt gttcttagcg gtgaagtggg     1980

ttgtaaaaaa actacatctt cataatcttt ataaaatttt agagcatgta acagcacttt     2040

atcgcttgtg gtatcatctt gtgcaaggct aattgggcgt tttaaaatat caacattttg     2100

actttttgca taatttaaaa tttcatcact atcactgctt acaacaactt tactaatgct     2160

tttagcattt agtgcagctt tgatcgtgta gtaaattaaa ggtttattgt ttaataaaac     2220

caaattttta tttttaatac cctttgagcc accacgagca gggattattg ctaagctcat     2280

tttatatcct taaaaacttt ttgtgtgctg agtttaaaaa aatctccgct ttgtaaatat     2340

tcaaaaaata attttgagct atctaaaatc tctaacttag cgctaaataa atcttgtttt     2400

ttatgaatag tgttaatagc ttttagtatt tcatcactat ttgcattaac ttttagtgta     2460

ttttcattgc caagtcttcc attttgtctt gagccaacta aaatccctgc tgtttttaag     2520

tataaggcct cttttaaaat acaacttgaa ttacctatta taaaatcagc attttttaac     2580

aaagttataa aatactcaaa tctaagcgat ggaaaaagct taaatctagg gttattttta     2640

aactcttcat agctttgcaa gattaattca aaacctaaat cattatttgg ataaataaca     2700

atataatttt tattactttg tatcagtgct tttactaaat tgtctgcttg atttttaatg     2760

ctagtaattt cagttgtaac aggatgaaac ataagcaaag cgtagttttc ataatttata     2820

tcataatatt tttttgcttc gctaagtgaa attttattat cgtttaaaag ttctaaatca     2880

ggcgaaccta tgataaaaat agatttttca tcttctccaa gctgcattaa acgccttttt     2940

gcaaactcat catttactaa atgaatatga gctagttttg atatagcgtg gcgtaagcta     3000

tcgtcaatag ttcctgaaat ctctccgcct tcaatatgcg ctactaagat attatttaat     3060

gctccaacaa tagctgctgc taaaggctca attctatctc catgtactac gattaaatca     3120

ggttttagct catttgcata ccttgaaaat ccatcaattg tagtagctaa agccttatca     3180

gtttgataat atttatcata atttataaat tcataaatat ttttaaagcc atttttataa     3240

agttctttaa ctgtatagcc aaaattttta cttaagtgca ttcctgttgc aaagatgtaa     3300

agttcaaatt cgcttgagtt ttgcaccctg tacattaaag atttaatctt agaataatca     3360

gccctagagc ctgttataaa aaggattttt ttcacgcaaa atcctcatag cttaactgag     3420

catcattttc tatatctctt aatgcttttt tgcctaaaat attttcaaat tcagccgcac     3480

taattccacc aagtccaggt cttttaaccc aaatattatc catagataaa acttcgcctt     3540

ttttaatatc tttaatgcta actacacttg caaaggcaaa atcaattgta acttgttctt     3600

gtttagccgc ttttttactt tcattatttc ctcttattat agccatttgc tcactttgta     3660

taattagctc ttttaaagcc tttgtatcca tagaacaaac tatatcaggg ccacttctat     3720

gcatactatc agtaaaatgt ctttcaagca cacaagctcc aagtacaact gcacctaaac     3780

acgcaagatt atctgttgtg tggtcgctta agcctaccat acaagaaaat tcttttttta     3840

actcaagcat agcgtttaat cttacaagat tatgcggggt tgggtaaaga ttggtcgtgt     3900

gcattaaaac aaaaggaatt tcattgtcta ataagatttt tacagttggt tttatacttt     3960

caatactatt cattcctgtg ctaactatca taggcttttt aaaggctgct atgtgtttaa     4020

taagcggata attattacac tcacctgaac caatcttaaa agcactaact cccatatctt     4080

ctaagcggtt cgcacctgca cgagaaaaag gtgtgctaag ataaacaaga cctaattttt     4140

ctgtgtattc tttaagtgct agctcatctt tataatccaa agcacatttt tgcataatct     4200

cataaatgct tatttttgca ttaccaggaa ttactttttt agcggcctta ctcatctcat     4260

cttcaacaat atgagtttga tgctttataa tcttagcacc tgcgctaaag gctgcatcta     4320

ccataatttt agctagttct aaactgccat tatgattaat gcctatttca ggtacgacta     4380

agggtgcttt ttcttcactt atgattatat tttgtatttt tatttctttc atttattttc     4440

ctccttagtc gacggtaccc ttaagccacc ccccagcgga acgacacttt aagatgcgta     4500

ttgccgccac ccccccaaaa cggcaggatc ttacgtttga tcttacgcag gatgttctta     4560

agacgattca caagaagctt ctcttcaata ttcttgaact caagcaactt ttcctcgata     4620

tttttgaact ctaaaagttt ctcctcgatg tttttaaatt ccagaagctt ctcctcaagg     4680

ggaagcgata caatcaggtc acttgtatag ccgatcggtt tatcaagcaa ctcgaagtgg     4740

tattttgctt gcgggttcag tgccaggatg tgacgaagcg gagagttcgg tgctaaggcg     4800

taaagttttg catacttttg acacaggcag attgtgaaca tgtcatagta aatgttgtgg     4860

caatcggcca tgtgattgct gaagtgctgg gactgactct catcgtagaa gtggccccag     4920

cttgacgcac caaagtcaat cccgaccaag taaatctcct tataccccaa aaccacggcc     4980

gacaacagca ttaagactcc ggcactcaat tctttactat aagtttttaa gtggtacttc     5040

acatggcgaa ggatttcctc atggccctta aaaaggctga atgtgtcaca aacaaatggg     5100

tagtgggcct tcaaataacc ctccaccgga aggatcgtct gataatcgcc gtcgaagtca     5160

tggcggtctg tcgagacact gcagaagaag cgttcgatgg aatattcacc gttgtccatc     5220

agatattgag ctgtttgcat ttgagaagat aacacacagg gattgaagaa tacgccttta     5280

atctcacgtc caaggtaata cttatcctcg aaataaaact gattacagcg aaagacttcg     5340

aaatccttgg gaaataaact atagtccagg tctttgatgg atggcccgtt ccccgcaata     5400

attaagggtt tcttcatatg tatatctcct tcttgaattc taacaattga ttgaatgtat     5460

gcaaataaat gcatacacca taggtgtggt ttaatttgat gccctttttc agggctggaa     5520

tgtgtaagag cggggttatt tatgctgttg tttttttgtt actcgggaag ggctttacct     5580

cttccgcata aacgcttcca tcagcgttta tagttaaaaa aatctttcgg aactggtttt     5640

gcgcttaccc caaccaacag gggatttgct gctttccatt gagcctgttt ctctgcgcga     5700

cgttcgcggc ggcgtgtttg tgcatccatc tggattctcc tgtcagttag ctttggtggt     5760

gtgtggcagt tgtagtcctg aacgaaaacc ccccgcgatt ggcacattgg cagctaatcc     5820

ggaatcgcac ttacggccaa tgcttcgttt cgtatcacac accccaaagc cttctgcttt     5880

gaatgctgcc cttcttcagg gcttaatttt taagagcgtc accttcatgg tggtcagtgc     5940

gtcctgctga tgtgctcagt atcaccgcca gtggtattta tgtcaacacc gccagagata     6000

atttatcacc gcagatggtt atctgtatgt tttttatatg aatttatttt ttgcaggggg     6060

gcattgtttg gtaggtgaga gatcaattct gcattaatga atcggccaac gcgcggggag     6120

aggcggtttg cgtattgggc gctcttccgc tgctagcgga gtgtatactg gcttactatg     6180

ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc     6240

ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct     6300

acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct     6360

ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc     6420

cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga     6480

aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct     6540

cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat     6600

tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga     6660

accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     6720

ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag     6780

tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc     6840

ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc     6900

cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc     6960

aagaagatca tcttattaat cagataaaat atttctaggc ggccgcgaac gaaaactcac     7020

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     7080

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc     7140

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     7200

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg     7260

ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc     7320

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     7380

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     7440

ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct     7500

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta     7560

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg     7620

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga     7680

ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt     7740

gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca     7800

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt     7860

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt     7920

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga     7980

aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt     8040

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc     8100

gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa     8160

cctataaaaa taggcgtatc acgaggccct ttcgtc                               8196


<210>  13
<211>  385
<212>  PRT
<213>  Pasteurella dagmatis

<400>  13

Met Thr Ile Tyr Leu Asp Pro Ala Ser Leu Pro Thr Leu Asn Gln Leu 
1               5                   10                  15      


Met His Phe Thr Lys Glu Ser Glu Asp Lys Glu Thr Ala Arg Ile Phe 
            20                  25                  30          


Gly Phe Ser Arg Phe Lys Leu Pro Glu Lys Ile Thr Glu Gln Tyr Asn 
        35                  40                  45              


Asn Ile His Phe Val Glu Ile Lys Asn Asn Arg Pro Thr Glu Asp Ile 
    50                  55                  60                  


Phe Thr Ile Leu Asp Gln Tyr Pro Glu Lys Leu Glu Leu Asp Leu His 
65                  70                  75                  80  


Leu Asn Ile Ala His Ser Ile Gln Leu Phe His Pro Ile Leu Gln Tyr 
                85                  90                  95      


Arg Phe Lys His Pro Asp Arg Ile Ser Ile Lys Ser Leu Asn Leu Tyr 
            100                 105                 110         


Asp Asp Gly Thr Met Glu Tyr Val Asp Leu Glu Lys Glu Glu Asn Lys 
        115                 120                 125             


Asp Ile Lys Ser Ala Ile Lys Lys Ala Glu Lys Gln Leu Ser Asp Tyr 
    130                 135                 140                 


Leu Leu Thr Gly Lys Ile Asn Phe Asp Asn Pro Thr Leu Ala Arg Tyr 
145                 150                 155                 160 


Val Trp Gln Ser Gln Tyr Pro Val Lys Tyr His Phe Leu Ser Thr Glu 
                165                 170                 175     


Tyr Phe Glu Lys Ala Glu Phe Leu Gln Pro Leu Lys Thr Tyr Leu Ala 
            180                 185                 190         


Gly Lys Tyr Gln Lys Met Asp Trp Ser Ala Tyr Glu Lys Leu Ser Pro 
        195                 200                 205             


Glu Gln Gln Thr Phe Tyr Leu Lys Leu Val Gly Phe Ser Asp Glu Thr 
    210                 215                 220                 


Lys Gln Leu Phe His Thr Glu Gln Thr Lys Phe Ile Phe Thr Gly Thr 
225                 230                 235                 240 


Thr Thr Trp Glu Gly Asn Thr Asp Ile Arg Glu Tyr Tyr Ala Lys Gln 
                245                 250                 255     


Gln Leu Asn Leu Leu Lys His Phe Thr His Ser Glu Gly Asp Leu Phe 
            260                 265                 270         


Ile Gly Asp Gln Tyr Lys Ile Tyr Phe Lys Gly His Pro Arg Gly Gly 
        275                 280                 285             


Asp Ile Asn Asp Tyr Ile Leu Lys His Ala Lys Asp Ile Thr Asn Ile 
    290                 295                 300                 


Pro Ala Asn Ile Ser Phe Glu Ile Leu Met Met Thr Gly Leu Leu Pro 
305                 310                 315                 320 


Asp Lys Val Gly Gly Val Ala Ser Ser Leu Tyr Phe Ser Leu Pro Lys 
                325                 330                 335     


Glu Lys Ile Ser His Ile Ile Phe Thr Ser Asn Lys Lys Ile Lys Asn 
            340                 345                 350         


Lys Glu Asp Ala Leu Asn Asp Pro Tyr Val Arg Val Met Leu Arg Leu 
        355                 360                 365             


Gly Met Ile Asp Lys Ser Gln Ile Ile Phe Trp Asp Ser Leu Lys Gln 
    370                 375                 380                 


Leu 
385 


<210>  14
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant form of PdST

<400>  14

Met Thr Ile Tyr Leu Asp His Ala Ser Leu Pro Thr Leu Asn Gln Leu 
1               5                   10                  15      


Met His Phe Thr Lys Glu Ser Glu Asp Lys Glu Thr Ala Arg Ile Phe 
            20                  25                  30          


Gly Phe Ser Arg Phe Lys Leu Pro Glu Lys Ile Thr Glu Gln Tyr Asn 
        35                  40                  45              


Asn Ile His Phe Val Glu Ile Lys Asn Asn Arg Pro Thr Glu Asp Ile 
    50                  55                  60                  


Phe Thr Ile Leu Asp Gln Tyr Pro Glu Lys Leu Glu Leu Asp Leu His 
65                  70                  75                  80  


Leu Asn Ile Ala His Ser Ile Gln Leu Phe His Pro Ile Leu Gln Tyr 
                85                  90                  95      


Arg Phe Lys His Pro Asp Arg Ile Ser Ile Lys Ser Leu Asn Leu Tyr 
            100                 105                 110         


Asp Asp Gly Thr Ala Glu Tyr Val Asp Leu Glu Lys Glu Glu Asn Lys 
        115                 120                 125             


Asp Ile Lys Ser Ala Ile Lys Lys Ala Glu Lys Gln Leu Ser Asp Tyr 
    130                 135                 140                 


Leu Leu Thr Gly Lys Ile Asn Phe Asp Asn Pro Thr Leu Ala Arg Tyr 
145                 150                 155                 160 


Val Trp Gln Ser Gln Tyr Pro Val Lys Tyr His Phe Leu Ser Thr Glu 
                165                 170                 175     


Tyr Phe Glu Lys Ala Glu Phe Leu Gln Pro Leu Lys Thr Tyr Leu Ala 
            180                 185                 190         


Gly Lys Tyr Gln Lys Met Asp Trp Ser Ala Tyr Glu Lys Leu Ser Pro 
        195                 200                 205             


Glu Gln Gln Thr Phe Tyr Leu Lys Leu Val Gly Phe Ser Asp Glu Thr 
    210                 215                 220                 


Lys Gln Leu Phe His Thr Glu Gln Thr Lys Phe Ile Phe Thr Gly Thr 
225                 230                 235                 240 


Thr Thr Trp Glu Gly Asn Thr Asp Ile Arg Glu Tyr Tyr Ala Lys Gln 
                245                 250                 255     


Gln Leu Asn Leu Leu Lys His Phe Thr His Ser Glu Gly Asp Leu Phe 
            260                 265                 270         


Ile Gly Asp Gln Tyr Lys Ile Tyr Phe Lys Gly His Pro Arg Gly Gly 
        275                 280                 285             


Asp Ile Asn Asp Tyr Ile Leu Lys His Ala Lys Asp Ile Thr Asn Ile 
    290                 295                 300                 


Pro Ala Asn Ile Ser Phe Glu Ile Leu Met Met Thr Gly Leu Leu Pro 
305                 310                 315                 320 


Asp Lys Val Gly Gly Val Ala Ser Ser Leu Tyr Phe Ser Leu Pro Lys 
                325                 330                 335     


Glu Lys Ile Ser His Ile Ile Phe Thr Ser Asn Lys Lys Ile Lys Asn 
            340                 345                 350         


Lys Glu Asp Ala Leu Asn Asp Pro Tyr Val Arg Val Met Leu Arg Leu 
        355                 360                 365             


Gly Met Ile Asp Lys Ser Gln Ile Ile Phe Trp Asp Ser Leu Lys Gln 
    370                 375                 380                 


Leu 
385 


<210>  15
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant form of PdST

<400>  15

Met Glu Ile Tyr Leu Asp His Ala Ser Leu Pro Ser Leu Asn Met Ile 
1               5                   10                  15      


Leu Asn Leu Val Glu Asn Lys Asn Asn Glu Lys Val Glu Arg Ile Ile 
            20                  25                  30          


Gly Phe Glu Arg Phe Asp Phe Asn Lys Glu Ile Leu Asn Ser Phe Ser 
        35                  40                  45              


Lys Glu Arg Ile Glu Phe Ser Lys Val Ser Ile Leu Asp Ile Lys Glu 
    50                  55                  60                  


Phe Ser Asp Lys Leu Tyr Leu Asn Ile Glu Lys Ser Asp Thr Pro Val 
65                  70                  75                  80  


Asp Leu Ile Ile His Thr Asn Leu Asp His Ser Val Arg Ser Leu Leu 
                85                  90                  95      


Ser Ile Phe Lys Thr Leu Ser Pro Leu Phe His Lys Ile Asn Ile Glu 
            100                 105                 110         


Lys Leu Tyr Leu Tyr Asp Asp Gly Ser Ala Asn Tyr Val Asp Leu Tyr 
        115                 120                 125             


Gln His Arg Gln Glu Asn Ile Ser Ala Ile Leu Ile Glu Ala Gln Lys 
    130                 135                 140                 


Lys Leu Lys Asp Ala Leu Glu Asn Arg Glu Thr Asp Thr Asp Lys Leu 
145                 150                 155                 160 


His Ser Leu Thr Arg Tyr Thr Trp His Lys Ile Phe Pro Thr Glu Tyr 
                165                 170                 175     


Ile Leu Leu Arg Pro Asp Tyr Leu Asp Ile Asp Glu Lys Met Gln Pro 
            180                 185                 190         


Leu Lys His Phe Leu Ser Asp Thr Ile Val Ser Met Asp Leu Ser Arg 
        195                 200                 205             


Phe Ser His Phe Ser Lys Asn Gln Lys Glu Leu Phe Leu Lys Ile Thr 
    210                 215                 220                 


His Phe Asp Gln Asn Ile Phe Asn Glu Leu Asn Ile Gly Thr Lys Asn 
225                 230                 235                 240 


Lys Glu Tyr Lys Thr Phe Ile Phe Thr Gly Thr Thr Thr Trp Glu Lys 
                245                 250                 255     


Asp Lys Lys Lys Arg Leu Asn Asn Ala Lys Leu Gln Thr Glu Ile Leu 
            260                 265                 270         


Glu Ser Phe Ile Lys Pro Asn Gly Lys Phe Tyr Leu Gly Asn Asp Ile 
        275                 280                 285             


Lys Ile Phe Phe Lys Gly His Pro Lys Gly Asp Asp Ile Asn Asp Tyr 
    290                 295                 300                 


Ile Ile Arg Lys Thr Gly Ala Glu Lys Ile Pro Ala Asn Ile Pro Phe 
305                 310                 315                 320 


Glu Val Leu Met Met Thr Asn Ser Leu Pro Asp Tyr Val Gly Gly Ile 
                325                 330                 335     


Met Ser Thr Val Tyr Phe Ser Leu Pro Pro Lys Asn Ile Asp Lys Val 
            340                 345                 350         


Val Phe Leu Gly Ser Glu Lys Ile Lys Asn Glu Asn Asp Ala Lys Ser 
        355                 360                 365             


Gln Thr Leu Ser Lys Leu Met Leu Met Leu Asn Val Ile Thr Pro Glu 
    370                 375                 380                 


Gln Ile Phe Phe Glu Glu Met Pro Asn Pro Ile Asn Phe 
385                 390                 395         


<210>  16
<211>  399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant form of BstE

<400>  16

Met Leu Ile Gln Gln Asn Leu Glu Ile Tyr Leu Asp His Ala Thr Ile 
1               5                   10                  15      


Pro Ser Leu Ala Cys Phe Met His Phe Ile Gln His Lys Asp Asp Val 
            20                  25                  30          


Asp Ser Ile Arg Leu Phe Gly Leu Ala Arg Phe Asp Ile Pro Gln Ser 
        35                  40                  45              


Ile Ile Asp Arg Tyr Pro Ala Asn His Leu Phe Tyr His Asn Ile Asp 
    50                  55                  60                  


Asn Arg Asp Leu Thr Ala Val Leu Asn Gln Leu Ala Asp Ile Leu Ala 
65                  70                  75                  80  


Gln Glu Asn Lys Arg Phe Gln Ile Asn Leu His Leu Asn Leu Phe His 
                85                  90                  95      


Ser Ile Asp Leu Phe Phe Ala Ile Tyr Pro Ile Tyr Gln Gln Tyr Gln 
            100                 105                 110         


His Lys Ile Ser Thr Ile Gln Leu Gln Leu Tyr Asp Asp Gly Ser Ala 
        115                 120                 125             


Gly Ile Val Thr Gln His Ser Leu Cys Lys Ile Ala Asp Leu Glu Gln 
    130                 135                 140                 


Leu Ile Leu Gln His Lys Asn Val Leu Leu Glu Leu Leu Thr Lys Gly 
145                 150                 155                 160 


Thr Ala Asn Val Pro Asn Pro Thr Leu Leu Arg Tyr Leu Trp Asn Asn 
                165                 170                 175     


Ile Ile Asp Ser Gln Phe His Leu Ile Ser Asp His Phe Leu Gln His 
            180                 185                 190         


Pro Lys Leu Gln Pro Leu Lys Arg Leu Leu Lys Arg Tyr Thr Ile Leu 
        195                 200                 205             


Asp Phe Thr Cys Tyr Pro Arg Phe Asn Ala Glu Gln Lys Gln Leu Leu 
    210                 215                 220                 


Lys Glu Ile Leu His Ile Ser Asn Glu Leu Glu Asn Leu Leu Lys Leu 
225                 230                 235                 240 


Leu Lys Gln His Asn Thr Phe Leu Phe Thr Gly Thr Thr Ala Phe Asn 
                245                 250                 255     


Leu Asp Gln Glu Lys Leu Asp Leu Leu Thr Gln Leu His Ile Leu Leu 
            260                 265                 270         


Leu Asn Glu His Gln Asn Pro His Ser Thr His Tyr Ile Gly Asn Asn 
        275                 280                 285             


Tyr Leu Leu Leu Ile Lys Gly His Ala Asn Ser Pro Ala Leu Asn His 
    290                 295                 300                 


Thr Leu Ala Leu His Phe Pro Asp Ala Ile Phe Leu Pro Ala Asn Ile 
305                 310                 315                 320 


Pro Phe Glu Ile Phe Ala Met Leu Gly Phe Thr Pro Asn Lys Met Gly 
                325                 330                 335     


Gly Phe Ala Ser Thr Ser Tyr Ile Asn Tyr Pro Thr Glu Asn Ile Asn 
            340                 345                 350         


His Leu Phe Phe Leu Thr Ser Asp Gln Pro Ser Ile Arg Thr Lys Trp 
        355                 360                 365             


Leu Asp Tyr Glu Lys Gln Phe Gly Leu Met Tyr Ser Leu Leu Ala Met 
    370                 375                 380                 


Gln Lys Ile Asn Glu Asp Gln Ala Phe Met Cys Thr Ile His Asn 
385                 390                 395                 


<210>  17
<211>  8433
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  17
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg      240

cctcctcaac ctgtatattc gtaaaccacg cccaatggga gctgtctcag gtttgttcct      300

gattggttac ggcgcgtttc gcatcattgt tgagtttttc cgccagcccg acgcgcagtt      360

taccggtgcc tgggtgcagt acatcagcat ggggcaaatt ctttccatcc cgatgattgt      420

cgcgggtgtg atcatgatgg tctgggcata tcgtcgcagc ccacagcaac acgtttcctg      480

aggaaccatg aaacagtatt tagaactgat gcaaaaagtg ctcgacgaag gcacacagaa      540

aaacgaccgt accggaaccg gaacgctttc catttttggt catcagatgc gttttaacct      600

gcaagatgga ttcccgctgg tgacaactaa acgttgccac ctgcgttcca tcatccatga      660

actgctgtgg tttctgcagg gcgacactaa cattgcttat ctacacgaaa acaatgtcac      720

catctgggac gaatgggccg atgaaaacgg cgacctcggg ccagtgtatg gtaaacagtg      780

gcgcgcctgg ccaacgccag atggtcgtca tattgaccag atcactacgg tactgaacca      840

gctgaaaaac gacccggatt cgcgccgcat tattgtttca gcgtggaacg taggcgaact      900

ggataaaatg gcgctggcac cgtgccatgc attcttccag ttctatgtgg cagacggcaa      960

actctcttgc cagctttatc agcgctcctg tgacgtcttc ctcggcctgc cgttcaacat     1020

tgccagctac gcgttattgg tgcatatgat ggcgcagcag tgcgatctgg aagtgggtga     1080

ttttgtctgg accggtggcg acacgcatct gtacagcaac catatggatc aaactcatct     1140

gcaattaagc cgcgaaccgc gtccgctgcc gaagttgatt atcaaacgta aacccgaatc     1200

catcttcgac taccgtttcg aagactttga gattgaaggc tacgatccgc atccgggcat     1260

taaagcgccg gtggctatct aattacgaaa catcctgcca gagccgacgc cagtgtgcgt     1320

cggttttttt accctccgtt aaattcttcg agacgccttc ccgaaggcgc cattcgccat     1380

tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc     1440

tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt     1500

cacgacgttg taaaacgacg gccagtgcca agcttactgc tcacaagaaa aaaggcacgt     1560

catctgacgt gcctttttta tttgtactac cctgtacgat tactgcaggt cgacttattt     1620

tttccatatc tgttcaacct tttttaaatc ctccaaacag tcaatatcta aacttgagct     1680

ttcgtccatt aaaaaatgct tggttttgct ttgtaaaaag ctaggattgt ttaaaaattc     1740

ttttatcttt aaaatataaa ttgcaccatt gctcatataa gttttaggca atttttgcct     1800

tggcataaaa ggatattcat cattacaaat ccctgctaaa tcgccacaat cattacaaac     1860

aaaggctttt agaattttat tatcacattc gcttacgcta attagggcat ttgcattgct     1920

atttttataa agattaaaag cttcattaat atgaatattt gttcttagcg gtgaagtggg     1980

ttgtaaaaaa actacatctt cataatcttt ataaaatttt agagcatgta acagcacttt     2040

atcgcttgtg gtatcatctt gtgcaaggct aattgggcgt tttaaaatat caacattttg     2100

actttttgca taatttaaaa tttcatcact atcactgctt acaacaactt tactaatgct     2160

tttagcattt agtgcagctt tgatcgtgta gtaaattaaa ggtttattgt ttaataaaac     2220

caaattttta tttttaatac cctttgagcc accacgagca gggattattg ctaagctcat     2280

tttatatcct taaaaacttt ttgtgtgctg agtttaaaaa aatctccgct ttgtaaatat     2340

tcaaaaaata attttgagct atctaaaatc tctaacttag cgctaaataa atcttgtttt     2400

ttatgaatag tgttaatagc ttttagtatt tcatcactat ttgcattaac ttttagtgta     2460

ttttcattgc caagtcttcc attttgtctt gagccaacta aaatccctgc tgtttttaag     2520

tataaggcct cttttaaaat acaacttgaa ttacctatta taaaatcagc attttttaac     2580

aaagttataa aatactcaaa tctaagcgat ggaaaaagct taaatctagg gttattttta     2640

aactcttcat agctttgcaa gattaattca aaacctaaat cattatttgg ataaataaca     2700

atataatttt tattactttg tatcagtgct tttactaaat tgtctgcttg atttttaatg     2760

ctagtaattt cagttgtaac aggatgaaac ataagcaaag cgtagttttc ataatttata     2820

tcataatatt tttttgcttc gctaagtgaa attttattat cgtttaaaag ttctaaatca     2880

ggcgaaccta tgataaaaat agatttttca tcttctccaa gctgcattaa acgccttttt     2940

gcaaactcat catttactaa atgaatatga gctagttttg atatagcgtg gcgtaagcta     3000

tcgtcaatag ttcctgaaat ctctccgcct tcaatatgcg ctactaagat attatttaat     3060

gctccaacaa tagctgctgc taaaggctca attctatctc catgtactac gattaaatca     3120

ggttttagct catttgcata ccttgaaaat ccatcaattg tagtagctaa agccttatca     3180

gtttgataat atttatcata atttataaat tcataaatat ttttaaagcc atttttataa     3240

agttctttaa ctgtatagcc aaaattttta cttaagtgca ttcctgttgc aaagatgtaa     3300

agttcaaatt cgcttgagtt ttgcaccctg tacattaaag atttaatctt agaataatca     3360

gccctagagc ctgttataaa aaggattttt ttcacgcaaa atcctcatag cttaactgag     3420

catcattttc tatatctctt aatgcttttt tgcctaaaat attttcaaat tcagccgcac     3480

taattccacc aagtccaggt cttttaaccc aaatattatc catagataaa acttcgcctt     3540

ttttaatatc tttaatgcta actacacttg caaaggcaaa atcaattgta acttgttctt     3600

gtttagccgc ttttttactt tcattatttc ctcttattat agccatttgc tcactttgta     3660

taattagctc ttttaaagcc tttgtatcca tagaacaaac tatatcaggg ccacttctat     3720

gcatactatc agtaaaatgt ctttcaagca cacaagctcc aagtacaact gcacctaaac     3780

acgcaagatt atctgttgtg tggtcgctta agcctaccat acaagaaaat tcttttttta     3840

actcaagcat agcgtttaat cttacaagat tatgcggggt tgggtaaaga ttggtcgtgt     3900

gcattaaaac aaaaggaatt tcattgtcta ataagatttt tacagttggt tttatacttt     3960

caatactatt cattcctgtg ctaactatca taggcttttt aaaggctgct atgtgtttaa     4020

taagcggata attattacac tcacctgaac caatcttaaa agcactaact cccatatctt     4080

ctaagcggtt cgcacctgca cgagaaaaag gtgtgctaag ataaacaaga cctaattttt     4140

ctgtgtattc tttaagtgct agctcatctt tataatccaa agcacatttt tgcataatct     4200

cataaatgct tatttttgca ttaccaggaa ttactttttt agcggcctta ctcatctcat     4260

cttcaacaat atgagtttga tgctttataa tcttagcacc tgcgctaaag gctgcatcta     4320

ccataatttt agctagttct aaactgccat tatgattaat gcctatttca ggtacgacta     4380

agggtgcttt ttcttcactt atgattatat tttgtatttt tatttctttc atttattttc     4440

ctccttagtc gacggtacac ttaaaagttg atcggattcg gcatttcttc aaaaaaaatc     4500

tgttccggag taataacgtt cagcatcagc atcagtttgc tcagggtctg ggatttggca     4560

tcgttttcat ttttgatttt ttcggagccc aggaatacta ctttatcgat gtttttcggt     4620

ggcaggctaa agtacacggt agacatgatg ccacctacat agtccggcag agagttggtc     4680

atcatcagaa cttcgaacgg gatgttggcc gggatttttt ccgcaccggt tttgcggata     4740

atatagtcgt tgatatcgtc gcctttcggg tggcctttga agaagatttt aatgtcgtta     4800

cccagataga atttgccgtt cggtttgata aaggattcca ggatttccgt ctgcagtttc     4860

gcgttgttca gacgtttttt tttatctttc tcccaggtgg tggtaccggt gaagatgaaa     4920

gttttatatt ctttgttttt ggtaccaatg ttcagttcgt tgaagatgtt ctgatcaaag     4980

tgagtaattt tcaggaacag ttctttctgg ttcttagaga agtgagaaaa gcggctcaga     5040

tccatgctaa caatggtgtc agacaggaaa tgcttcagcg gctgcatctt ttcgtcgata     5100

tccagatagt ccgggcgcag cagaatgtat tcggtcggaa aaatcttgtg ccaagtgtaa     5160

cgggtcagag aatgcagttt gtcggtatca gtttcacggt tctccagtgc gtccttcagc     5220

tttttctgtg cttcgatcag gattgcgctg atgttttcct gacgatgctg atacagatct     5280

acgtagttac cagagccgtc gtcgtacaga tacagctttt cgatgttgat cttgtggaac     5340

agcggggaca gggttttgaa aatagacagc agagaacgaa cagaatgatc caggttagtg     5400

tgaataatca ggtccaccgg ggtatcgctt ttttcgatgt tcaggtacag tttgtcgctg     5460

aactccttaa tgtccagaat gctcactttg gagaactcga tgcgctcttt ggagaaagag     5520

ttcagaattt ctttgttgaa atcgaagcgt tcaaaaccga tgatacgttc cactttctca     5580

ttatttttgt tttcaaccag attcaggatc atgttcaggc taggcaggga tgcgtagtcc     5640

aggtaaattt ccatatgtat atctccttct tgaattctaa caattgattg aatgtatgca     5700

aataaatgca tacaccatag gtgtggttta atttgatgcc ctttttcagg gctggaatgt     5760

gtaagagcgg ggttatttat gctgttgttt ttttgttact cgggaagggc tttacctctt     5820

ccgcataaac gcttccatca gcgtttatag ttaaaaaaat ctttcggaac tggttttgcg     5880

cttaccccaa ccaacagggg atttgctgct ttccattgag cctgtttctc tgcgcgacgt     5940

tcgcggcggc gtgtttgtgc atccatctgg attctcctgt cagttagctt tggtggtgtg     6000

tggcagttgt agtcctgaac gaaaaccccc cgcgattggc acattggcag ctaatccgga     6060

atcgcactta cggccaatgc ttcgtttcgt atcacacacc ccaaagcctt ctgctttgaa     6120

tgctgccctt cttcagggct taatttttaa gagcgtcacc ttcatggtgg tcagtgcgtc     6180

ctgctgatgt gctcagtatc accgccagtg gtatttatgt caacaccgcc agagataatt     6240

tatcaccgca gatggttatc tgtatgtttt ttatatgaat ttattttttg caggggggca     6300

ttgtttggta ggtgagagat caattctgca ttaatgaatc ggccaacgcg cggggagagg     6360

cggtttgcgt attgggcgct cttccgctgc tagcggagtg tatactggct tactatgttg     6420

gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt     6480

gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg actcgctacg     6540

ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga     6600

agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat     6660

aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac     6720

ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct     6780

gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc     6840

acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc     6900

ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga     6960

aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct     7020

tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc     7080

caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct     7140

gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag     7200

aagatcatct tattaatcag ataaaatatt tctaggcggc cgcgaacgaa aactcacgtt     7260

aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa     7320

aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat     7380

gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct     7440

gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg     7500

caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag     7560

ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta     7620

attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg     7680

ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg     7740

gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct     7800

ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta     7860

tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg     7920

gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc     7980

cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg     8040

gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga     8100

tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg     8160

ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat     8220

gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc     8280

tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca     8340

catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct     8400

ataaaaatag gcgtatcacg aggccctttc gtc                                  8433


<210>  18
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstC

<400>  18

Met Glu Ile Tyr Leu Asp Tyr Ala Ser Leu Pro Ser Leu Asn Met Ile 
1               5                   10                  15      


Leu Asn Leu Val Glu Asn Lys Asn Asn Glu Lys Val Glu Arg Ile Ile 
            20                  25                  30          


Gly Phe Glu Arg Phe Asp Phe Asn Lys Glu Ile Leu Asn Ser Phe Ser 
        35                  40                  45              


Lys Glu Arg Ile Glu Phe Ser Lys Val Ser Ile Leu Asp Ile Lys Glu 
    50                  55                  60                  


Phe Ser Asp Lys Leu Tyr Leu Asn Ile Glu Lys Ser Asp Thr Pro Val 
65                  70                  75                  80  


Asp Leu Ile Ile His Thr Asn Leu Asp His Ser Val Arg Ser Leu Leu 
                85                  90                  95      


Ser Ile Phe Lys Thr Leu Ser Pro Leu Phe His Lys Ile Asn Ile Glu 
            100                 105                 110         


Lys Leu Tyr Leu Tyr Asp Asp Gly Ser Gly Asn Tyr Val Asp Leu Tyr 
        115                 120                 125             


Gln His Arg Gln Glu Asn Ile Ser Ala Ile Leu Ile Glu Ala Gln Lys 
    130                 135                 140                 


Lys Leu Lys Asp Ala Leu Glu Asn Arg Glu Thr Asp Thr Asp Lys Leu 
145                 150                 155                 160 


His Ser Leu Thr Arg Tyr Thr Trp His Lys Ile Phe Pro Thr Glu Tyr 
                165                 170                 175     


Ile Leu Leu Arg Pro Asp Tyr Leu Asp Ile Asp Glu Lys Met Gln Pro 
            180                 185                 190         


Leu Lys His Phe Leu Ser Asp Thr Ile Val Ser Met Asp Leu Ser Arg 
        195                 200                 205             


Phe Ser His Phe Ser Lys Asn Gln Lys Glu Leu Phe Leu Lys Ile Thr 
    210                 215                 220                 


His Phe Asp Gln Asn Ile Phe Asn Glu Leu Asn Ile Gly Thr Lys Asn 
225                 230                 235                 240 


Lys Glu Tyr Lys Thr Phe Ile Phe Thr Gly Thr Thr Thr Trp Glu Lys 
                245                 250                 255     


Asp Lys Lys Lys Arg Leu Asn Asn Ala Lys Leu Gln Thr Glu Ile Leu 
            260                 265                 270         


Glu Ser Phe Ile Lys Pro Asn Gly Lys Phe Tyr Leu Gly Asn Asp Ile 
        275                 280                 285             


Lys Ile Phe Phe Lys Gly His Pro Lys Gly Asp Asp Ile Asn Asp Tyr 
    290                 295                 300                 


Ile Ile Arg Lys Thr Gly Ala Glu Lys Ile Pro Ala Asn Ile Pro Phe 
305                 310                 315                 320 


Glu Val Leu Met Met Thr Asn Ser Leu Pro Asp Tyr Val Gly Gly Ile 
                325                 330                 335     


Met Ser Thr Val Tyr Phe Ser Leu Pro Pro Lys Asn Ile Asp Lys Val 
            340                 345                 350         


Val Phe Leu Gly Ser Glu Lys Ile Lys Asn Glu Asn Asp Ala Lys Ser 
        355                 360                 365             


Gln Thr Leu Ser Lys Leu Met Leu Met Leu Asn Val Ile Thr Pro Glu 
    370                 375                 380                 


Gln Ile Phe Phe Glu Glu Met Pro Asn Pro Ile Asn Phe 
385                 390                 395         


<210>  19
<211>  417
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstC

<400>  19

Met Arg Lys Ile Ile Thr Phe Phe Ser Leu Phe Phe Ser Ile Ser Ala 
1               5                   10                  15      


Trp Cys Gln Lys Met Glu Ile Tyr Leu Asp Tyr His Ser Leu Pro Ser 
            20                  25                  30          


Leu Asn Met Ile Leu Asn Leu Val Glu Asn Lys Asn Asn Glu Lys Val 
        35                  40                  45              


Glu Arg Ile Ile Gly Phe Glu Arg Phe Asp Phe Asn Lys Glu Ile Leu 
    50                  55                  60                  


Asn Ser Phe Ser Lys Glu Arg Ile Glu Phe Ser Lys Val Ser Ile Leu 
65                  70                  75                  80  


Asp Ile Lys Glu Phe Ser Asp Lys Leu Tyr Leu Asn Ile Glu Lys Ser 
                85                  90                  95      


Asp Thr Pro Val Asp Leu Ile Ile His Thr Asn Leu Asp His Ser Val 
            100                 105                 110         


Arg Ser Leu Leu Ser Ile Phe Lys Thr Leu Ser Pro Leu Phe His Lys 
        115                 120                 125             


Ile Asn Ile Glu Lys Leu Tyr Leu Tyr Asp Asp Gly Ser Ala Asn Tyr 
    130                 135                 140                 


Val Asp Leu Tyr Gln His Arg Gln Glu Asn Ile Ser Ala Ile Leu Ile 
145                 150                 155                 160 


Glu Ala Gln Lys Lys Leu Lys Asp Ala Leu Glu Asn Arg Glu Thr Asp 
                165                 170                 175     


Thr Asp Lys Leu His Ser Leu Thr Arg Tyr Thr Trp His Lys Ile Phe 
            180                 185                 190         


Pro Thr Glu Tyr Ile Leu Leu Arg Pro Asp Tyr Leu Asp Ile Asp Glu 
        195                 200                 205             


Lys Met Gln Pro Leu Lys His Phe Leu Ser Asp Thr Ile Val Ser Met 
    210                 215                 220                 


Asp Leu Ser Arg Phe Ser His Phe Ser Lys Asn Gln Lys Glu Leu Phe 
225                 230                 235                 240 


Leu Lys Ile Thr His Phe Asp Gln Asn Ile Phe Asn Glu Leu Asn Ile 
                245                 250                 255     


Gly Thr Lys Asn Lys Glu Tyr Lys Thr Phe Ile Phe Thr Gly Thr Thr 
            260                 265                 270         


Thr Trp Glu Lys Asp Lys Lys Lys Arg Leu Asn Asn Ala Lys Leu Gln 
        275                 280                 285             


Thr Glu Ile Leu Glu Ser Phe Ile Lys Pro Asn Gly Lys Phe Tyr Leu 
    290                 295                 300                 


Gly Asn Asp Ile Lys Ile Phe Phe Lys Gly His Pro Lys Gly Asp Asp 
305                 310                 315                 320 


Ile Asn Asp Tyr Ile Ile Arg Lys Thr Gly Ala Glu Lys Ile Pro Ala 
                325                 330                 335     


Asn Ile Pro Phe Glu Val Leu Met Met Thr Asn Ser Leu Pro Asp Tyr 
            340                 345                 350         


Val Gly Gly Ile Met Ser Thr Val Tyr Phe Ser Leu Pro Pro Lys Asn 
        355                 360                 365             


Ile Asp Lys Val Val Phe Leu Gly Ser Glu Lys Ile Lys Asn Glu Asn 
    370                 375                 380                 


Asp Ala Lys Ser Gln Thr Leu Ser Lys Leu Met Leu Met Leu Asn Val 
385                 390                 395                 400 


Ile Thr Pro Glu Gln Ile Phe Phe Glu Glu Met Pro Asn Pro Ile Asn 
                405                 410                 415     


Phe 
    


<210>  20
<211>  264
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstD

<400>  20

Met Phe Lys Ile Lys Ser Tyr Gly Lys Asn Pro Gln Leu Gln Ala Val 
1               5                   10                  15      


Asp Ile Tyr Ile Asp His Ala Thr Ile Pro Ser Leu Ser Tyr Phe Leu 
            20                  25                  30          


His Phe Leu Lys His Lys His Asp His Gln Arg Leu Arg Leu Phe Ser 
        35                  40                  45              


Leu Ala Arg Phe Glu Met Pro Gln Thr Val Ile Glu Gln Tyr Glu Gly 
    50                  55                  60                  


Ile Ile Gln Phe Ser Arg Asn Val Glu His Asn Val Glu Pro Leu Leu 
65                  70                  75                  80  


Glu Gln Leu Gln Thr Ile Leu Ser Gln Glu Gly Lys Gln Phe Glu Leu 
                85                  90                  95      


His Leu His Leu Asn Leu Phe His Ser Phe Glu Met Phe Leu Asn Leu 
            100                 105                 110         


Ser Pro Thr Tyr Thr Lys Tyr Lys Glu Lys Ile Ser Lys Ile Val Leu 
        115                 120                 125             


His Leu Tyr Asp Asp Gly Ser Ala Gly Val Met Lys Gln Tyr Gln Leu 
    130                 135                 140                 


Gln Lys Ser Ser Ser Leu Val Gln Asp Leu Ala Ala Thr Lys Ala Ser 
145                 150                 155                 160 


Leu Val Ser Leu Phe Glu Asn Gly Glu Gly Ser Phe Ser Gln Ile Asp 
                165                 170                 175     


Leu Ile Arg Tyr Val Trp Asn Ala Val Leu Glu Thr His Tyr Tyr Leu 
            180                 185                 190         


Leu Ser Asp His Phe Leu Leu Asp Glu Lys Leu Gln Pro Leu Lys Ala 
        195                 200                 205             


Glu Leu Gly His Tyr Gln Leu Leu Asn Leu Ser Thr Tyr Gln Tyr Leu 
    210                 215                 220                 


Ser Ser Glu Asp Leu Leu Trp Leu Lys Gln Ile Leu Lys Ile Asp Ala 
225                 230                 235                 240 


Glu Leu Glu Ser Leu Met Gln Lys Leu Thr Ala Gln Pro Val Tyr Phe 
                245                 250                 255     


Phe Ser Gly Thr Thr Phe Leu Gly 
            260                 


<210>  21
<211>  399
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstE

<400>  21

Met Leu Ile Gln Gln Asn Leu Glu Ile Tyr Leu Asp His Ala Thr Ile 
1               5                   10                  15      


Pro Ser Leu Ala Cys Phe Met His Phe Ile Gln His Lys Asp Asp Val 
            20                  25                  30          


Asp Ser Ile Arg Leu Phe Gly Leu Ala Arg Phe Asp Ile Pro Gln Ser 
        35                  40                  45              


Ile Ile Asp Arg Tyr Pro Ala Asn His Leu Phe Tyr His Asn Ile Asp 
    50                  55                  60                  


Asn Arg Asp Leu Thr Ala Val Leu Asn Gln Leu Ala Asp Ile Leu Ala 
65                  70                  75                  80  


Gln Glu Asn Lys Arg Phe Gln Ile Asn Leu His Leu Asn Leu Phe His 
                85                  90                  95      


Ser Ile Asp Leu Phe Phe Ala Ile Tyr Pro Ile Tyr Gln Gln Tyr Gln 
            100                 105                 110         


His Lys Ile Ser Thr Ile Gln Leu Gln Leu Tyr Asp Asp Gly Ser Ala 
        115                 120                 125             


Gly Ile Val Thr Gln His Ser Leu Cys Lys Ile Ala Asp Leu Glu Gln 
    130                 135                 140                 


Leu Ile Leu Gln His Lys Asn Val Leu Leu Glu Leu Leu Thr Lys Gly 
145                 150                 155                 160 


Thr Ala Asn Val Pro Asn Pro Thr Leu Leu Arg Tyr Leu Trp Asn Asn 
                165                 170                 175     


Ile Ile Asp Ser Gln Phe His Leu Ile Ser Asp His Phe Leu Gln His 
            180                 185                 190         


Pro Lys Leu Gln Pro Leu Lys Arg Leu Leu Lys Arg Tyr Thr Ile Leu 
        195                 200                 205             


Asp Phe Thr Cys Tyr Pro Arg Phe Asn Ala Glu Gln Lys Gln Leu Leu 
    210                 215                 220                 


Lys Glu Ile Leu His Ile Ser Asn Glu Leu Glu Asn Leu Leu Lys Leu 
225                 230                 235                 240 


Leu Lys Gln His Asn Thr Phe Leu Phe Thr Gly Thr Thr Ala Phe Asn 
                245                 250                 255     


Leu Asp Gln Glu Lys Leu Asp Leu Leu Thr Gln Leu His Ile Leu Leu 
            260                 265                 270         


Leu Asn Glu His Gln Asn Pro His Ser Thr His Tyr Ile Gly Asn Asn 
        275                 280                 285             


Tyr Leu Leu Leu Ile Lys Gly His Ala Asn Ser Pro Ala Leu Asn His 
    290                 295                 300                 


Thr Leu Ala Leu His Phe Pro Asp Ala Ile Phe Leu Pro Ala Asn Ile 
305                 310                 315                 320 


Pro Phe Glu Ile Phe Ala Met Leu Gly Phe Thr Pro Asn Lys Met Gly 
                325                 330                 335     


Gly Phe Ala Ser Thr Ser Tyr Ile Asn Tyr Pro Thr Glu Asn Ile Asn 
            340                 345                 350         


His Leu Phe Phe Leu Thr Ser Asp Gln Pro Ser Ile Arg Thr Lys Trp 
        355                 360                 365             


Leu Asp Tyr Glu Lys Gln Phe Gly Leu Met Tyr Ser Leu Leu Ala Met 
    370                 375                 380                 


Gln Lys Ile Asn Glu Asp Gln Ala Phe Met Cys Thr Ile His Asn 
385                 390                 395                 


<210>  22
<211>  498
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstH

<400>  22

Met Lys Arg Leu Phe Arg Leu Phe Leu Cys Leu Ala Leu Leu Ser Gly 
1               5                   10                  15      


Thr Ala Ala Cys Ser Asp Asp Glu Val Ser Gln Asn Leu Ile Val Ile 
            20                  25                  30          


Asn Gly Gly Glu His Phe Leu Ser Leu Asp Gly Leu Ala Arg Ala Gly 
        35                  40                  45              


Lys Ile Ser Val Leu Ala Pro Ala Pro Trp Arg Val Thr Lys Ala Ala 
    50                  55                  60                  


Gly Asp Thr Trp Phe Arg Leu Ser Ala Thr Glu Gly Pro Ala Gly Tyr 
65                  70                  75                  80  


Ser Glu Val Glu Leu Ser Leu Asp Glu Asn Pro Gly Ala Ala Arg Ser 
                85                  90                  95      


Ala Gln Leu Ala Phe Ala Cys Gly Asp Ala Ile Val Pro Phe Arg Leu 
            100                 105                 110         


Ser Gln Gly Ala Leu Ser Ala Gly Tyr Asp Ser Pro Asp Tyr Tyr Phe 
        115                 120                 125             


Tyr Val Thr His Gly Thr Met Pro Thr Leu Tyr Ala Gly Ile His Leu 
    130                 135                 140                 


Leu Ser His Asp Lys Pro Gly Tyr Val Phe Tyr Ser Arg Ser Lys Thr 
145                 150                 155                 160 


Phe Asp Pro Ala Glu Phe Pro Ala Arg Ala Glu Val Thr Thr Ala Ala 
                165                 170                 175     


Asp Arg Thr Ala Asp Ala Thr Gln Ala Glu Met Glu Ala Met Ala Arg 
            180                 185                 190         


Glu Met Lys Arg Arg Ile Leu Glu Ile Asn Ser Ala Asp Pro Thr Ala 
        195                 200                 205             


Val Phe Gly Leu Tyr Val Asp Asp Leu Arg Cys Arg Ile Gly Tyr Asp 
    210                 215                 220                 


Trp Phe Val Ala Gln Gly Ile Asp Ser Ala Arg Val Lys Val Ser Met 
225                 230                 235                 240 


Leu Ser Asp Gly Thr Gly Thr Tyr Asn Ala Phe Tyr Asn Tyr Phe Gly 
                245                 250                 255     


Asp Ala Ala Thr Ala Glu Gln Asn Trp Glu Ser Tyr Ala Ser Glu Val 
            260                 265                 270         


Glu Ala Leu Asp Trp Asn His Gly Gly Arg Tyr Pro Glu Thr Arg Ser 
        275                 280                 285             


Leu Pro Glu Phe Glu Ser Tyr Thr Trp Pro Tyr Tyr Leu Ser Thr Arg 
    290                 295                 300                 


Pro Asp Tyr Arg Leu Val Val Gln Asp Gly Ser Leu Leu Glu Ser Ser 
305                 310                 315                 320 


Cys Pro Phe Ile Thr Glu Lys Leu Gly Glu Met Glu Ile Glu Ser Ile 
                325                 330                 335     


Gln Pro Tyr Glu Met Leu Ser Ala Leu Pro Glu Ser Ser Arg Lys Arg 
            340                 345                 350         


Phe Tyr Asp Met Ala Gly Phe Asp Tyr Asp Lys Phe Ala Ala Leu Phe 
        355                 360                 365             


Asp Ala Ser Pro Lys Lys Asn Leu Ile Ile Ile Gly Thr Ser His Ala 
    370                 375                 380                 


Asp Asp Ala Ser Ala Arg Leu Gln Arg Asp Tyr Val Ala Arg Ile Met 
385                 390                 395                 400 


Glu Gln Tyr Gly Ala Gln Tyr Asp Val Phe Phe Lys Pro His Pro Ala 
                405                 410                 415     


Asp Thr Thr Ser Ala Gly Tyr Glu Thr Glu Phe Pro Gly Leu Thr Leu 
            420                 425                 430         


Leu Pro Gly Gln Met Pro Phe Glu Ile Phe Val Trp Ser Leu Ile Asp 
        435                 440                 445             


Arg Val Asp Met Ile Gly Gly Tyr Pro Ser Thr Val Phe Leu Thr Val 
    450                 455                 460                 


Pro Val Asp Lys Val Arg Phe Ile Phe Ala Ala Asp Ala Ala Ser Leu 
465                 470                 475                 480 


Val Arg Pro Leu Asn Ile Leu Phe Arg Asp Ala Thr Asp Val Glu Trp 
                485                 490                 495     


Met Gln 
        


<210>  23
<211>  410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstI

<400>  23

Met Glu Phe Cys Lys Met Ala Thr Thr Gln Lys Ile Cys Val Tyr Leu 
1               5                   10                  15      


Asp His Ala Thr Ile Pro Ser Leu Asn Tyr Ile Leu His Phe Ala Gln 
            20                  25                  30          


His Phe Glu Asp Gln Glu Thr Ile Arg Leu Phe Gly Leu Ser Arg Phe 
        35                  40                  45              


His Ile Pro Glu Ser Val Ile Gln Arg Tyr Pro Lys Gly Val Val Gln 
    50                  55                  60                  


Phe Tyr Pro Asn Gln Glu Lys Asp Phe Ser Ala Leu Leu Leu Ala Leu 
65                  70                  75                  80  


Lys Asn Ile Leu Ile Glu Val Lys Gln Gln Gln Arg Lys Cys Glu Ile 
                85                  90                  95      


Glu Leu His Leu Asn Leu Phe His Tyr Gln Leu Leu Leu Leu Pro Phe 
            100                 105                 110         


Leu Ser Leu Tyr Leu Asp Thr Gln Asp Tyr Cys His Leu Thr Leu Lys 
        115                 120                 125             


Phe Tyr Asp Asp Gly Ser Ala Ala Ile Ser Ala Leu Gln Glu Leu Ala 
    130                 135                 140                 


Leu Ala Pro Asp Leu Ala Ala Gln Ile Gln Phe Glu Lys Gln Gln Phe 
145                 150                 155                 160 


Asp Glu Leu Val Val Lys Lys Ser Phe Lys Leu Ser Leu Leu Ser Arg 
                165                 170                 175     


Tyr Phe Trp Gly Lys Leu Phe Glu Ser Glu Tyr Ile Trp Phe Asn Gln 
            180                 185                 190         


Ala Ile Leu Gln Lys Ala Glu Leu Gln Ile Leu Lys Gln Glu Ile Ser 
        195                 200                 205             


Ser Ser Arg Gln Met Asp Phe Ala Ile Tyr Gln Gln Met Ser Asp Glu 
    210                 215                 220                 


Gln Lys Gln Leu Val Leu Glu Ile Leu Asn Ile Asp Leu Asn Lys Val 
225                 230                 235                 240 


Ala Tyr Leu Lys Gln Leu Met Glu Asn Gln Pro Ser Phe Leu Phe Leu 
                245                 250                 255     


Gly Thr Thr Leu Phe Asn Ile Thr Gln Glu Thr Lys Thr Trp Leu Met 
            260                 265                 270         


Gln Met His Val Asp Leu Ile Gln Gln Tyr Cys Leu Pro Ser Gly Gln 
        275                 280                 285             


Phe Phe Asn Asn Lys Ala Gly Tyr Leu Cys Phe Tyr Lys Gly His Pro 
    290                 295                 300                 


Asn Glu Lys Glu Met Asn Gln Met Ile Leu Ser Gln Phe Lys Asn Leu 
305                 310                 315                 320 


Ile Ala Leu Pro Asp Asp Ile Pro Leu Glu Ile Leu Leu Leu Leu Gly 
                325                 330                 335     


Val Ile Pro Ser Lys Val Gly Gly Phe Ala Ser Ser Ala Leu Phe Asn 
            340                 345                 350         


Phe Thr Pro Ala Gln Ile Glu Asn Ile Ile Phe Phe Thr Pro Arg Tyr 
        355                 360                 365             


Phe Glu Lys Asp Asn Arg Leu His Ala Thr Gln Tyr Arg Leu Met Gln 
    370                 375                 380                 


Gly Leu Ile Glu Leu Gly Tyr Leu Asp Ala Glu Lys Ser Val Thr His 
385                 390                 395                 400 


Phe Glu Ile Met Gln Leu Leu Thr Lys Glu 
                405                 410 


<210>  24
<211>  367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstJ

<400>  24

Met Leu Val Asn Asn Gln Ser His Asn Pro Lys Leu Ile Cys Trp Gln 
1               5                   10                  15      


Arg His Pro Val Asn Asp Glu Ala Leu Leu Gln Gly Ile Asn Ala Ala 
            20                  25                  30          


Ser Phe Val Ser Ile Ala Ser Leu Cys Gln His Ala Ala Thr Leu Leu 
        35                  40                  45              


Ala Gly His Pro His Ser His Ile Thr Ile Tyr Gly Asn Thr Tyr Trp 
    50                  55                  60                  


Ser Lys Asp Leu Ala Arg Leu Ile Arg Tyr Leu Thr Arg Ile Ser Gly 
65                  70                  75                  80  


Val Glu Ile Lys Lys Leu Glu Leu Ile Asp Asp Gly Ser Ser Glu Tyr 
                85                  90                  95      


Gln Lys Met Phe Tyr Trp Gln Arg Leu His Ser Glu Glu Gln Thr Arg 
            100                 105                 110         


Asp Leu Ala Thr Gly Leu Lys Asn Leu Lys Ser Tyr Leu Ser Gly Asn 
        115                 120                 125             


Asp Asn Lys Leu Leu Arg Leu Leu Thr Gly His Ser Asn Lys Leu Pro 
    130                 135                 140                 


Arg Arg Leu Ser Ser Phe Met Asn Trp His Gln Leu Phe Pro Thr Thr 
145                 150                 155                 160 


Tyr His Met Leu Arg Met Asp Tyr Leu Asp Lys Pro Glu Leu His Gln 
                165                 170                 175     


Leu Lys Gln Tyr Leu Gly Asn Asn Ala Gln Gln Ile Arg Trp Asn Tyr 
            180                 185                 190         


Ile Ala Asp Asn Leu Phe Asp Asp Glu Gln Gln Ser Leu Phe Tyr Gln 
        195                 200                 205             


Leu Leu Gly Ile Ser Leu Ala Glu Gln Lys Gln Leu Arg Ala Gly Arg 
    210                 215                 220                 


Gln Gln Leu His Asp Phe Met Phe Ile Gly Val Asp Ser Ser Asn Ala 
225                 230                 235                 240 


Ser Ser Lys Leu Gln Ile Asn Val Ile Ala Asp Ser Arg Gln Glu Ser 
                245                 250                 255     


Gly Ile Ile Pro Thr Ile Thr Ala Lys Lys Met Leu Phe Lys Gly His 
            260                 265                 270         


Pro Phe Ala Asn Phe Asn Gln Thr Ile Val Asp Ala His Gln Met Gly 
        275                 280                 285             


Glu Met Pro Ala Met Ile Pro Phe Glu Thr Leu Ile Met Thr Gly Asn 
    290                 295                 300                 


Leu Pro Gln Lys Val Gly Gly Met Ala Ser Ser Leu Tyr Phe Ser Leu 
305                 310                 315                 320 


Pro Asn Asn Tyr His Ile Glu Tyr Ile Val Phe Ser Gly Ser Lys Lys 
                325                 330                 335     


Asp Leu Glu Gln His Ala Leu Leu Gln Ile Met Leu Tyr Leu Lys Val 
            340                 345                 350         


Ile Ser Pro Glu Arg Val Tyr Phe Ser Glu Gln Phe Lys Ser Cys 
        355                 360                 365         


<210>  25
<211>  318
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstM

<400>  25

Met Lys Lys Pro Leu Ile Ile Ala Gly Asn Gly Pro Ser Ile Lys Asp 
1               5                   10                  15      


Leu Asp Tyr Ser Leu Phe Pro Lys Asp Phe Glu Val Phe Arg Cys Asn 
            20                  25                  30          


Gln Phe Tyr Phe Glu Asp Lys Tyr Tyr Leu Gly Arg Glu Ile Lys Gly 
        35                  40                  45              


Val Phe Phe Asn Pro Cys Val Leu Ser Ser Gln Met Gln Thr Ala Gln 
    50                  55                  60                  


Tyr Leu Met Asp Asn Gly Glu Tyr Ser Ile Glu Arg Phe Phe Cys Ser 
65                  70                  75                  80  


Val Ser Thr Asp Arg His Asp Phe Asp Gly Asp Tyr Gln Thr Ile Leu 
                85                  90                  95      


Pro Val Glu Gly Tyr Leu His Ala His Tyr Pro Phe Val Cys Asp Thr 
            100                 105                 110         


Phe Ser Leu Phe Lys Gly His Glu Glu Ile Leu Arg His Val Lys Tyr 
        115                 120                 125             


His Leu Lys Thr Tyr Ser Lys Glu Leu Ser Ala Gly Val Leu Met Leu 
    130                 135                 140                 


Leu Ser Ala Val Val Leu Gly Tyr Lys Glu Ile Tyr Leu Val Gly Ile 
145                 150                 155                 160 


Asp Phe Gly Ala Ser Ser Trp Gly His Phe Tyr Asp Glu Ser Gln Ser 
                165                 170                 175     


Gln His Phe Ser Asn His Met Ala Asp Cys His Asn Ile Tyr Tyr Asp 
            180                 185                 190         


Met Phe Thr Ile Cys Leu Cys Gln Lys Tyr Ala Lys Leu Tyr Ala Leu 
        195                 200                 205             


Ala Pro Asn Ser Pro Leu Arg His Ile Leu Ala Leu Asn Pro Ala Ala 
    210                 215                 220                 


Lys Tyr His Phe Glu Leu Leu Asp Lys Pro Ile Gly Tyr Thr Ser Asp 
225                 230                 235                 240 


Leu Ile Val Ser Leu Pro Leu Glu Glu Lys Leu Leu Glu Phe Lys Asn 
                245                 250                 255     


Ile Glu Glu Lys Leu Leu Glu Phe Lys Asn Ile Glu Glu Lys Leu Leu 
            260                 265                 270         


Glu Phe Lys Asn Ile Glu Glu Lys Leu Leu Val Asn Arg Leu Lys Asn 
        275                 280                 285             


Ile Leu Arg Lys Ile Lys Arg Lys Ile Leu Pro Phe Trp Gly Gly Gly 
    290                 295                 300                 


Gly Asn Thr His Leu Lys Val Ser Phe Arg Trp Gly Val Ala 
305                 310                 315             


<210>  26
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstN

<400>  26

Met Ser Glu Lys Ile Phe Ser Gln Val Asp Glu Lys Asn Gln Lys Lys 
1               5                   10                  15      


Pro Leu Ile Ile Ala Gly Asn Gly Pro Ser Ile Lys Asp Leu Asp Tyr 
            20                  25                  30          


Ser Leu Phe Pro Lys Asp Phe Asp Val Phe Arg Cys Asn Gln Phe Tyr 
        35                  40                  45              


Phe Glu Asp Lys Tyr Tyr Leu Gly Lys Glu Val Lys Gly Val Phe Phe 
    50                  55                  60                  


Asn Pro Cys Val Phe His Asn Gln Met Asn Thr Ala Lys His Leu Ile 
65                  70                  75                  80  


Asp Asn Asn Glu Tyr Tyr Ile Glu Gln Phe Phe Cys Ser Val Ser Lys 
                85                  90                  95      


Glu Gln His Asp Phe Asn Gly Asp Tyr Gln Thr Ile Leu Ser Val Asp 
            100                 105                 110         


Glu Tyr Leu His Ala Asn Tyr Pro Phe Val Arg Asp Thr Phe Ser Leu 
        115                 120                 125             


Phe Gly Glu His Glu Glu Ile Leu Asn His Val Lys Tyr His Leu Lys 
    130                 135                 140                 


Thr Tyr Ser Lys Glu Leu Ser Ala Gly Val Leu Met Leu Leu Ser Ala 
145                 150                 155                 160 


Ile Val Leu Gly Tyr Lys Glu Ile Tyr Leu Val Gly Val Asp Phe Gly 
                165                 170                 175     


Ala Asn Ser Trp Gly His Phe Tyr Asp Asp Asn Gln Ser Gln His Phe 
            180                 185                 190         


Ile Asn His Met Ala Asp Cys His Asn Ile Tyr Tyr Asp Met Leu Thr 
        195                 200                 205             


Ile Tyr Leu Cys Gln Lys Tyr Ala Lys Leu Tyr Ala Leu Val Pro Asn 
    210                 215                 220                 


Ser Pro Leu Asn His Leu Leu Pro Leu Asn Leu Ala Ala Asn His Val 
225                 230                 235                 240 


Phe Glu Leu Leu Asp Lys Pro Ile Gly Tyr Thr Ser Asp Leu Ile Val 
                245                 250                 255     


Ser Ser Pro Leu Glu Glu Lys Leu Leu Glu Ser Lys Asn Ile Asp Glu 
            260                 265                 270         


Arg Phe Ser Gln Asn Lys Ser Phe Lys Asn Tyr Leu Gln Arg Leu Lys 
        275                 280                 285             


Asp Lys Phe Leu Gln Met Ile Phe Arg Gly Gly Gly Val Ile Thr Ile 
    290                 295                 300                 


Pro Arg Val Ile Phe Lys Gly Lys Phe Ala 
305                 310                 


<210>  27
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstC

<400>  27

Met Glu Ile Tyr Leu Asp His Ala Ser Leu Pro Ser Leu Asn Met Ile 
1               5                   10                  15      


Leu Asn Leu Val Glu Asn Lys Asn Asn Glu Lys Val Glu Arg Ile Ile 
            20                  25                  30          


Gly Phe Glu Arg Phe Asp Phe Asn Lys Glu Ile Leu Asn Ser Phe Ser 
        35                  40                  45              


Lys Glu Arg Ile Glu Phe Ser Lys Val Ser Ile Leu Asp Ile Lys Glu 
    50                  55                  60                  


Phe Ser Asp Lys Leu Tyr Leu Asn Ile Glu Lys Ser Asp Thr Pro Val 
65                  70                  75                  80  


Asp Leu Ile Ile His Thr Asn Leu Asp His Ser Val Arg Ser Leu Leu 
                85                  90                  95      


Ser Ile Phe Lys Thr Leu Ser Pro Leu Phe His Lys Ile Asn Ile Glu 
            100                 105                 110         


Lys Leu Tyr Leu Tyr Asp Asp Gly Ser Val Asn Tyr Val Asp Leu Tyr 
        115                 120                 125             


Gln His Arg Gln Glu Asn Ile Ser Ala Ile Leu Ile Glu Ala Gln Lys 
    130                 135                 140                 


Lys Leu Lys Asp Ala Leu Glu Asn Arg Glu Thr Asp Thr Asp Lys Leu 
145                 150                 155                 160 


His Ser Leu Thr Arg Tyr Thr Trp His Lys Ile Phe Pro Thr Glu Tyr 
                165                 170                 175     


Ile Leu Leu Arg Pro Asp Tyr Leu Asp Ile Asp Glu Lys Met Gln Pro 
            180                 185                 190         


Leu Lys His Phe Leu Ser Asp Thr Ile Val Ser Met Asp Leu Ser Arg 
        195                 200                 205             


Phe Ser His Phe Ser Lys Asn Gln Lys Glu Leu Phe Leu Lys Ile Thr 
    210                 215                 220                 


His Phe Asp Gln Asn Ile Phe Asn Glu Leu Asn Ile Gly Thr Lys Asn 
225                 230                 235                 240 


Lys Glu Tyr Lys Thr Phe Ile Phe Thr Gly Thr Thr Thr Trp Glu Lys 
                245                 250                 255     


Asp Lys Lys Lys Arg Leu Asn Asn Ala Lys Leu Gln Thr Glu Ile Leu 
            260                 265                 270         


Glu Ser Phe Ile Lys Pro Asn Gly Lys Phe Tyr Leu Gly Asn Asp Ile 
        275                 280                 285             


Lys Ile Phe Phe Lys Gly His Pro Lys Gly Asp Asp Ile Asn Asp Tyr 
    290                 295                 300                 


Ile Ile Arg Lys Thr Gly Ala Glu Lys Ile Pro Ala Asn Ile Pro Phe 
305                 310                 315                 320 


Glu Val Leu Met Met Thr Asn Ser Leu Pro Asp Tyr Val Gly Gly Ile 
                325                 330                 335     


Met Ser Thr Val Tyr Phe Ser Leu Pro Pro Lys Asn Ile Asp Lys Val 
            340                 345                 350         


Val Phe Leu Gly Ser Glu Lys Ile Lys Asn Glu Asn Asp Ala Lys Ser 
        355                 360                 365             


Gln Thr Leu Ser Lys Leu Met Leu Met Leu Asn Val Ile Thr Pro Glu 
    370                 375                 380                 


Gln Ile Phe Phe Glu Glu Met Pro Asn Pro Ile Asn Phe 
385                 390                 395         


<210>  28
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstC

<400>  28

Met Glu Ile Tyr Leu Asp His Ala Ser Leu Pro Ser Leu Asn Met Ile 
1               5                   10                  15      


Leu Asn Leu Val Glu Asn Lys Asn Asn Glu Lys Val Glu Arg Ile Ile 
            20                  25                  30          


Gly Phe Glu Arg Phe Asp Phe Asn Lys Glu Ile Leu Asn Ser Phe Ser 
        35                  40                  45              


Lys Glu Arg Ile Glu Phe Ser Lys Val Ser Ile Leu Asp Ile Lys Glu 
    50                  55                  60                  


Phe Ser Asp Lys Leu Tyr Leu Asn Ile Glu Lys Ser Asp Thr Pro Val 
65                  70                  75                  80  


Asp Leu Ile Ile His Thr Asn Leu Asp His Ser Val Arg Ser Leu Leu 
                85                  90                  95      


Ser Ile Phe Lys Thr Leu Ser Pro Leu Phe His Lys Ile Asn Ile Glu 
            100                 105                 110         


Lys Leu Tyr Leu Tyr Asp Asp Gly Ser Leu Asn Tyr Val Asp Leu Tyr 
        115                 120                 125             


Gln His Arg Gln Glu Asn Ile Ser Ala Ile Leu Ile Glu Ala Gln Lys 
    130                 135                 140                 


Lys Leu Lys Asp Ala Leu Glu Asn Arg Glu Thr Asp Thr Asp Lys Leu 
145                 150                 155                 160 


His Ser Leu Thr Arg Tyr Thr Trp His Lys Ile Phe Pro Thr Glu Tyr 
                165                 170                 175     


Ile Leu Leu Arg Pro Asp Tyr Leu Asp Ile Asp Glu Lys Met Gln Pro 
            180                 185                 190         


Leu Lys His Phe Leu Ser Asp Thr Ile Val Ser Met Asp Leu Ser Arg 
        195                 200                 205             


Phe Ser His Phe Ser Lys Asn Gln Lys Glu Leu Phe Leu Lys Ile Thr 
    210                 215                 220                 


His Phe Asp Gln Asn Ile Phe Asn Glu Leu Asn Ile Gly Thr Lys Asn 
225                 230                 235                 240 


Lys Glu Tyr Lys Thr Phe Ile Phe Thr Gly Thr Thr Thr Trp Glu Lys 
                245                 250                 255     


Asp Lys Lys Lys Arg Leu Asn Asn Ala Lys Leu Gln Thr Glu Ile Leu 
            260                 265                 270         


Glu Ser Phe Ile Lys Pro Asn Gly Lys Phe Tyr Leu Gly Asn Asp Ile 
        275                 280                 285             


Lys Ile Phe Phe Lys Gly His Pro Lys Gly Asp Asp Ile Asn Asp Tyr 
    290                 295                 300                 


Ile Ile Arg Lys Thr Gly Ala Glu Lys Ile Pro Ala Asn Ile Pro Phe 
305                 310                 315                 320 


Glu Val Leu Met Met Thr Asn Ser Leu Pro Asp Tyr Val Gly Gly Ile 
                325                 330                 335     


Met Ser Thr Val Tyr Phe Ser Leu Pro Pro Lys Asn Ile Asp Lys Val 
            340                 345                 350         


Val Phe Leu Gly Ser Glu Lys Ile Lys Asn Glu Asn Asp Ala Lys Ser 
        355                 360                 365             


Gln Thr Leu Ser Lys Leu Met Leu Met Leu Asn Val Ile Thr Pro Glu 
    370                 375                 380                 


Gln Ile Phe Phe Glu Glu Met Pro Asn Pro Ile Asn Phe 
385                 390                 395         


<210>  29
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstC

<400>  29

Met Glu Ile Tyr Leu Asp His Ala Ser Leu Pro Ser Leu Asn Met Ile 
1               5                   10                  15      


Leu Asn Leu Val Glu Asn Lys Asn Asn Glu Lys Val Glu Arg Ile Ile 
            20                  25                  30          


Gly Phe Glu Arg Phe Asp Phe Asn Lys Glu Ile Leu Asn Ser Phe Ser 
        35                  40                  45              


Lys Glu Arg Ile Glu Phe Ser Lys Val Ser Ile Leu Asp Ile Lys Glu 
    50                  55                  60                  


Phe Ser Asp Lys Leu Tyr Leu Asn Ile Glu Lys Ser Asp Thr Pro Val 
65                  70                  75                  80  


Asp Leu Ile Ile His Thr Asn Leu Asp His Ser Val Arg Ser Leu Leu 
                85                  90                  95      


Ser Ile Phe Lys Thr Leu Ser Pro Leu Phe His Lys Ile Asn Ile Glu 
            100                 105                 110         


Lys Leu Tyr Leu Tyr Asp Asp Gly Ser Met Asn Tyr Val Asp Leu Tyr 
        115                 120                 125             


Gln His Arg Gln Glu Asn Ile Ser Ala Ile Leu Ile Glu Ala Gln Lys 
    130                 135                 140                 


Lys Leu Lys Asp Ala Leu Glu Asn Arg Glu Thr Asp Thr Asp Lys Leu 
145                 150                 155                 160 


His Ser Leu Thr Arg Tyr Thr Trp His Lys Ile Phe Pro Thr Glu Tyr 
                165                 170                 175     


Ile Leu Leu Arg Pro Asp Tyr Leu Asp Ile Asp Glu Lys Met Gln Pro 
            180                 185                 190         


Leu Lys His Phe Leu Ser Asp Thr Ile Val Ser Met Asp Leu Ser Arg 
        195                 200                 205             


Phe Ser His Phe Ser Lys Asn Gln Lys Glu Leu Phe Leu Lys Ile Thr 
    210                 215                 220                 


His Phe Asp Gln Asn Ile Phe Asn Glu Leu Asn Ile Gly Thr Lys Asn 
225                 230                 235                 240 


Lys Glu Tyr Lys Thr Phe Ile Phe Thr Gly Thr Thr Thr Trp Glu Lys 
                245                 250                 255     


Asp Lys Lys Lys Arg Leu Asn Asn Ala Lys Leu Gln Thr Glu Ile Leu 
            260                 265                 270         


Glu Ser Phe Ile Lys Pro Asn Gly Lys Phe Tyr Leu Gly Asn Asp Ile 
        275                 280                 285             


Lys Ile Phe Phe Lys Gly His Pro Lys Gly Asp Asp Ile Asn Asp Tyr 
    290                 295                 300                 


Ile Ile Arg Lys Thr Gly Ala Glu Lys Ile Pro Ala Asn Ile Pro Phe 
305                 310                 315                 320 


Glu Val Leu Met Met Thr Asn Ser Leu Pro Asp Tyr Val Gly Gly Ile 
                325                 330                 335     


Met Ser Thr Val Tyr Phe Ser Leu Pro Pro Lys Asn Ile Asp Lys Val 
            340                 345                 350         


Val Phe Leu Gly Ser Glu Lys Ile Lys Asn Glu Asn Asp Ala Lys Ser 
        355                 360                 365             


Gln Thr Leu Ser Lys Leu Met Leu Met Leu Asn Val Ile Thr Pro Glu 
    370                 375                 380                 


Gln Ile Phe Phe Glu Glu Met Pro Asn Pro Ile Asn Phe 
385                 390                 395         


<210>  30
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant version of BstC

<400>  30

Met Glu Ile Tyr Leu Asp His Ala Ser Leu Pro Ser Leu Asn Met Ile 
1               5                   10                  15      


Leu Asn Leu Val Glu Asn Lys Asn Asn Glu Lys Val Glu Arg Ile Ile 
            20                  25                  30          


Gly Phe Glu Arg Phe Asp Phe Asn Lys Glu Ile Leu Asn Ser Phe Ser 
        35                  40                  45              


Lys Glu Arg Ile Glu Phe Ser Lys Val Ser Ile Leu Asp Ile Lys Glu 
    50                  55                  60                  


Phe Ser Asp Lys Leu Tyr Leu Asn Ile Glu Lys Ser Asp Thr Pro Val 
65                  70                  75                  80  


Asp Leu Ile Ile His Thr Asn Leu Asp His Ser Val Arg Ser Leu Leu 
                85                  90                  95      


Ser Ile Phe Lys Thr Leu Ser Pro Leu Phe His Lys Ile Asn Ile Glu 
            100                 105                 110         


Lys Leu Tyr Leu Tyr Asp Asp Gly Ser Phe Asn Tyr Val Asp Leu Tyr 
        115                 120                 125             


Gln His Arg Gln Glu Asn Ile Ser Ala Ile Leu Ile Glu Ala Gln Lys 
    130                 135                 140                 


Lys Leu Lys Asp Ala Leu Glu Asn Arg Glu Thr Asp Thr Asp Lys Leu 
145                 150                 155                 160 


His Ser Leu Thr Arg Tyr Thr Trp His Lys Ile Phe Pro Thr Glu Tyr 
                165                 170                 175     


Ile Leu Leu Arg Pro Asp Tyr Leu Asp Ile Asp Glu Lys Met Gln Pro 
            180                 185                 190         


Leu Lys His Phe Leu Ser Asp Thr Ile Val Ser Met Asp Leu Ser Arg 
        195                 200                 205             


Phe Ser His Phe Ser Lys Asn Gln Lys Glu Leu Phe Leu Lys Ile Thr 
    210                 215                 220                 


His Phe Asp Gln Asn Ile Phe Asn Glu Leu Asn Ile Gly Thr Lys Asn 
225                 230                 235                 240 


Lys Glu Tyr Lys Thr Phe Ile Phe Thr Gly Thr Thr Thr Trp Glu Lys 
                245                 250                 255     


Asp Lys Lys Lys Arg Leu Asn Asn Ala Lys Leu Gln Thr Glu Ile Leu 
            260                 265                 270         


Glu Ser Phe Ile Lys Pro Asn Gly Lys Phe Tyr Leu Gly Asn Asp Ile 
        275                 280                 285             


Lys Ile Phe Phe Lys Gly His Pro Lys Gly Asp Asp Ile Asn Asp Tyr 
    290                 295                 300                 


Ile Ile Arg Lys Thr Gly Ala Glu Lys Ile Pro Ala Asn Ile Pro Phe 
305                 310                 315                 320 


Glu Val Leu Met Met Thr Asn Ser Leu Pro Asp Tyr Val Gly Gly Ile 
                325                 330                 335     


Met Ser Thr Val Tyr Phe Ser Leu Pro Pro Lys Asn Ile Asp Lys Val 
            340                 345                 350         


Val Phe Leu Gly Ser Glu Lys Ile Lys Asn Glu Asn Asp Ala Lys Ser 
        355                 360                 365             


Gln Thr Leu Ser Lys Leu Met Leu Met Leu Asn Val Ile Thr Pro Glu 
    370                 375                 380                 


Gln Ile Phe Phe Glu Glu Met Pro Asn Pro Ile Asn Phe 
385                 390                 395         


