                         SEQUENCE LISTING

<110>  INOVIO PHARMACEUTICALS, INC
       YAN, Jian
       SLAGER, Anna
       GARMAN, Bradley
       COOCH, Neil
 
<120>  CANCER VACCINES TARGETING MUC16 AND USES THEREOF

<130>  INO-1005WO / 104409.000446

<150>  US 62/598,314
<151>  2017-12-13

<150>  US 62/599,613
<151>  2017-12-15

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  4476
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic consensus MUC16 IRC + R59 DNA Coding Sequence

<400>  1
atggactgga cctggattct gttcctggtg gcagcagcaa cccgcgtgca ctccacagca       60

gcaggacctc tgctggtgcc attcaccctg aactttacca tcacaaatct gcagtacgag      120

gaggatatgc accacccagg cagcagaaag ttcaacacca cagagagggt gctgcagggc      180

ctgctgggac caatgtttaa gaataccagc gtgggcctgc tgtattccgg atgcaggctg      240

acactgctgc gctccgagaa ggacggagca gcaaccggcg tggatgccat ctgtacacac      300

aggctggacc ccaagagccc tggcctggat cgggagcagc tgtactggga gctgtcccag      360

ctgaccaacg gcatcaagga gctgggcccc tacacactgg accgcaacag cctgtatgtg      420

aatggcttta cccaccggag ctccgtgcca aataccagca cacccggcac ctccacagtg      480

gatctgggca cctccggcac accatctagc ctgccttctc caaccgcagc aggaccactg      540

ctggtgcctt tcacactgaa ctttaccatt accaatctgc agtatgaaga ggacatgagg      600

catcctggca gcagaaagtt caacacgaca gagagagtgc tgcaaggcct gctgaagcca      660

ctgtttaaga atacctctgt gggccccctg tatagtggct gtagactgac actgctgcgc      720

cccgaaaaag atggcgccgc cactggagtc gacgccatct gtacacacag actggacccc      780

aagtctcccg gcctgaacag agaacagctg tattgggaac tgagcaagct gaccaatggc      840

atcacagagc tgggccctta caccctggac agaaactctc tgtatgtgaa tggcttcacc      900

cacaggacat ctgtgcctac cacaagcacc ccaggcacct ccaccgtcga tctgggcacc      960

agcggcacac cttttagcct gccatcccct accaccgccg gaccactgct ggtgcccttc     1020

accctgaact ttaccataac caatctgcag tatgaggagg acatgcaccg gcccggcagc     1080

agaaagttca acactacaga gagagtgctg cagggcctgt tatcccctat ctttaagaat     1140

tcctctgtgg gcccactgta cagcggatgc aggctgacct ctctgcggcc cgaaaaagac     1200

ggagcagcaa caggaatgga tgccgtgtgc ctgtaccacc caaaccccaa gaggccaggc     1260

ctggatcgcg agcagctgta ttgggagctg agccagctga cccacaatat cacagagctg     1320

ggcccctact ctctggacag ggatagcctg tatgtgaacg gcttcaccca ccagaattcc     1380

gtgcccacca catctacacc tggcaccagc acagtgtact gggccaccac aggcacccct     1440

agctcctttc caggacacac agcacctgga ccactgctga tcccattcac cctgaacttt     1500

accattacaa atctgcacta tgaggagaac atgcagcacc ccggcagccg caagttcaat     1560

accacagagc gggtgctgca aggcctgctg aagccgctgt ttaagaacac cagcgtggga     1620

cctctgtact ctggctgtcg cctgacactg ctgcggcccg agaagcatgg cgcagcaacc     1680

ggcgtggacg ctatttgcac ccacagactg gaccctaagg gcccaggcct ggatagggag     1740

cgcctgtatt gggaactgtc tcagctgacc aattctatca cagagctggg accatacacc     1800

ctggacaggg attctctgta cgtgaacggc ttcaatccac ggtctagcgt gcccaccaca     1860

agcacccctg gcacctccac agtgcacctg gccacctctg gcacaccctc ctctctgcct     1920

ggacacaccg caagccccct gctggtgctg ttcacaatca actttaccat tactaatctg     1980

agatacgagg agaacatgca ccaccctggc tccagaaagt tcaatactac cgaaagggtg     2040

ctgcaaggcc tgctgcgccc agtgtttaag aacacttccg tgggccccct gtatagcggc     2100

tgtaggctga cactgctgcg ccccaagaag gatggcgcag caaccaaggt cgacgctatc     2160

tgtacataca ggcccgaccc taaatcccca ggcctggacc gggagcagct gtattgggag     2220

ctgtctcagc tgacccactc cattaccgaa ctgggaccct acaccctgga cagagattcc     2280

ctgtacgtga atggcttcac acagaggagc tccgtgccta ccacatccat cccaggaacc     2340

cctacagtgg acctgggcac ctccggaacc ccagtgtcta agccaggacc atctgccgca     2400

agccacctgc tgatcctgtt caccctgaac tttaccataa cgaatctgcg gtacgaggag     2460

aacatgtggc caggctcccg gaagttcaat actaccgaga gagtgctgca aggcctgctg     2520

aggcctctgt ttaagaacac tagcgtgggc ccactgtatt ccggctctcg gctgaccctg     2580

ctgcggcccg aaaaagatgg agaggccaca ggcgtggatg ccatctgcac ccacagacct     2640

gacccaacag gacctggcct ggacagggag cagctgtacc tggagctgtc acagctgact     2700

cactccatta ccgagctggg cccctatact ctggatagag actccctgta cgtcaacggc     2760

ttcacccacc ggtctagcgt gccaaccaca tctacaggcg tggtgagcga ggaacccttc     2820

accctgaact tcaccatcaa caatctgagg tacatggcag acatgggaca gccaggctct     2880

ctgaagttca acatcaccga taatgtgatg aagcacctgc tgagccctct gtttcagcgg     2940

agcagcctgg gagcaaggta caccggatgc cgcgtgatcg ccctgcggtc cgtgaagaac     3000

ggagcagaga cacgggtgga cctgctgtgc acatatctgc agcctctgag cggaccaggc     3060

ctgcccatca agcaggtgtt ccacgagctg tctcagcaga cccacggaat cacaaggctg     3120

ggaccctact ccctggacaa ggattctctg tacctgaacg gctataatga gcctggcctg     3180

gacgagcccc ctaccacacc caagcctgcc accacatttc tgccacccct gagcgaggca     3240

accacagcaa tgggatacca cctgaagacc ctgacactga acttcaccat cagcaatctg     3300

cagtattccc ccgatatggg caagggctct gccaccttta acagcacaga gggcgtgctg     3360

cagcacctgc tgcggcccct gttccagaag agctccatgg gcccttttta cctgggctgc     3420

cagctgatct ccctgaggcc tgaaaaagat ggagcagcaa ccggagtgga taccacatgt     3480

acataccacc cagaccccgt gggaccaggc ctggatatcc agcagctgta ctgggaactg     3540

tcccagctga ctcacggcgt gacacagctg ggcttctacg tgctggaccg cgattccctg     3600

tttatcaacg gctacgcccc tcagaatctg tctatccggg gcgagtatca gatcaacttc     3660

cacatcgtga actggaatct gagcaatcct gacccaacct ctagcgagta catcgccctg     3720

ctgcgcgaca tccaggataa ggtgaccaca ctgtataagg gctcccagct gcacgacacc     3780

ttccggtttt gcctggtgac caacctgaca atggattcta tgctggtgac agtgaaggcc     3840

ctgttctcct ctaacctgga ccccagcctg gtggagcagg tgtttctgga taagaccctg     3900

aatgccagct cccactggct gggctccacc taccagctgg tggacatcca cgtgacagag     3960

atggagccaa gcgtgtacca gcccacctct agctcctcta cacagcactt ctacctgaac     4020

tttaccataa ctaatctgcc ctatagccag gatatcgccc agcctggcac cacaaactac     4080

cagcggaaca agagaaatat cgaggacgcc ctgaaccagc tgttcagaaa tagctccatc     4140

aagtcctatt tctctgattg ccaggtgagc acctttaggt ccgtgcctaa ttctcaccac     4200

acaggcgtgg actccctgtg cgccttttct ccactggcaa ggagagtgga tagggtggca     4260

atctacgagg agttcctgag gatgacccgc gccggaacac agctgcaggc ctttaccctg     4320

gacagatcta gcgtgctggt ggatggctat agccctaaca ggaatgagcc actgaccggc     4380

aactccgacc tgcccttctg ggccatcatc ctgatctgtc tggcaggcct gctgggcctg     4440

atcacctgcc tgatctgtgg ctttctggtg tgataa                               4476


<210>  2
<211>  1490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic consensus MUC16 IRC + R59 Protein Sequence

<400>  2

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Thr Ala Ala Gly Pro Leu Leu Val Pro Phe Thr Leu Asn Phe 
            20                  25                  30          


Thr Ile Thr Asn Leu Gln Tyr Glu Glu Asp Met His His Pro Gly Ser 
        35                  40                  45              


Arg Lys Phe Asn Thr Thr Glu Arg Val Leu Gln Gly Leu Leu Gly Pro 
    50                  55                  60                  


Met Phe Lys Asn Thr Ser Val Gly Leu Leu Tyr Ser Gly Cys Arg Leu 
65                  70                  75                  80  


Thr Leu Leu Arg Ser Glu Lys Asp Gly Ala Ala Thr Gly Val Asp Ala 
                85                  90                  95      


Ile Cys Thr His Arg Leu Asp Pro Lys Ser Pro Gly Leu Asp Arg Glu 
            100                 105                 110         


Gln Leu Tyr Trp Glu Leu Ser Gln Leu Thr Asn Gly Ile Lys Glu Leu 
        115                 120                 125             


Gly Pro Tyr Thr Leu Asp Arg Asn Ser Leu Tyr Val Asn Gly Phe Thr 
    130                 135                 140                 


His Arg Ser Ser Val Pro Asn Thr Ser Thr Pro Gly Thr Ser Thr Val 
145                 150                 155                 160 


Asp Leu Gly Thr Ser Gly Thr Pro Ser Ser Leu Pro Ser Pro Thr Ala 
                165                 170                 175     


Ala Gly Pro Leu Leu Val Pro Phe Thr Leu Asn Phe Thr Ile Thr Asn 
            180                 185                 190         


Leu Gln Tyr Glu Glu Asp Met Arg His Pro Gly Ser Arg Lys Phe Asn 
        195                 200                 205             


Thr Thr Glu Arg Val Leu Gln Gly Leu Leu Lys Pro Leu Phe Lys Asn 
    210                 215                 220                 


Thr Ser Val Gly Pro Leu Tyr Ser Gly Cys Arg Leu Thr Leu Leu Arg 
225                 230                 235                 240 


Pro Glu Lys Asp Gly Ala Ala Thr Gly Val Asp Ala Ile Cys Thr His 
                245                 250                 255     


Arg Leu Asp Pro Lys Ser Pro Gly Leu Asn Arg Glu Gln Leu Tyr Trp 
            260                 265                 270         


Glu Leu Ser Lys Leu Thr Asn Gly Ile Thr Glu Leu Gly Pro Tyr Thr 
        275                 280                 285             


Leu Asp Arg Asn Ser Leu Tyr Val Asn Gly Phe Thr His Arg Thr Ser 
    290                 295                 300                 


Val Pro Thr Thr Ser Thr Pro Gly Thr Ser Thr Val Asp Leu Gly Thr 
305                 310                 315                 320 


Ser Gly Thr Pro Phe Ser Leu Pro Ser Pro Thr Thr Ala Gly Pro Leu 
                325                 330                 335     


Leu Val Pro Phe Thr Leu Asn Phe Thr Ile Thr Asn Leu Gln Tyr Glu 
            340                 345                 350         


Glu Asp Met His Arg Pro Gly Ser Arg Lys Phe Asn Thr Thr Glu Arg 
        355                 360                 365             


Val Leu Gln Gly Leu Leu Ser Pro Ile Phe Lys Asn Ser Ser Val Gly 
    370                 375                 380                 


Pro Leu Tyr Ser Gly Cys Arg Leu Thr Ser Leu Arg Pro Glu Lys Asp 
385                 390                 395                 400 


Gly Ala Ala Thr Gly Met Asp Ala Val Cys Leu Tyr His Pro Asn Pro 
                405                 410                 415     


Lys Arg Pro Gly Leu Asp Arg Glu Gln Leu Tyr Trp Glu Leu Ser Gln 
            420                 425                 430         


Leu Thr His Asn Ile Thr Glu Leu Gly Pro Tyr Ser Leu Asp Arg Asp 
        435                 440                 445             


Ser Leu Tyr Val Asn Gly Phe Thr His Gln Asn Ser Val Pro Thr Thr 
    450                 455                 460                 


Ser Thr Pro Gly Thr Ser Thr Val Tyr Trp Ala Thr Thr Gly Thr Pro 
465                 470                 475                 480 


Ser Ser Phe Pro Gly His Thr Ala Pro Gly Pro Leu Leu Ile Pro Phe 
                485                 490                 495     


Thr Leu Asn Phe Thr Ile Thr Asn Leu His Tyr Glu Glu Asn Met Gln 
            500                 505                 510         


His Pro Gly Ser Arg Lys Phe Asn Thr Thr Glu Arg Val Leu Gln Gly 
        515                 520                 525             


Leu Leu Lys Pro Leu Phe Lys Asn Thr Ser Val Gly Pro Leu Tyr Ser 
    530                 535                 540                 


Gly Cys Arg Leu Thr Leu Leu Arg Pro Glu Lys His Gly Ala Ala Thr 
545                 550                 555                 560 


Gly Val Asp Ala Ile Cys Thr His Arg Leu Asp Pro Lys Gly Pro Gly 
                565                 570                 575     


Leu Asp Arg Glu Arg Leu Tyr Trp Glu Leu Ser Gln Leu Thr Asn Ser 
            580                 585                 590         


Ile Thr Glu Leu Gly Pro Tyr Thr Leu Asp Arg Asp Ser Leu Tyr Val 
        595                 600                 605             


Asn Gly Phe Asn Pro Arg Ser Ser Val Pro Thr Thr Ser Thr Pro Gly 
    610                 615                 620                 


Thr Ser Thr Val His Leu Ala Thr Ser Gly Thr Pro Ser Ser Leu Pro 
625                 630                 635                 640 


Gly His Thr Ala Ser Pro Leu Leu Val Leu Phe Thr Ile Asn Phe Thr 
                645                 650                 655     


Ile Thr Asn Leu Arg Tyr Glu Glu Asn Met His His Pro Gly Ser Arg 
            660                 665                 670         


Lys Phe Asn Thr Thr Glu Arg Val Leu Gln Gly Leu Leu Arg Pro Val 
        675                 680                 685             


Phe Lys Asn Thr Ser Val Gly Pro Leu Tyr Ser Gly Cys Arg Leu Thr 
    690                 695                 700                 


Leu Leu Arg Pro Lys Lys Asp Gly Ala Ala Thr Lys Val Asp Ala Ile 
705                 710                 715                 720 


Cys Thr Tyr Arg Pro Asp Pro Lys Ser Pro Gly Leu Asp Arg Glu Gln 
                725                 730                 735     


Leu Tyr Trp Glu Leu Ser Gln Leu Thr His Ser Ile Thr Glu Leu Gly 
            740                 745                 750         


Pro Tyr Thr Leu Asp Arg Asp Ser Leu Tyr Val Asn Gly Phe Thr Gln 
        755                 760                 765             


Arg Ser Ser Val Pro Thr Thr Ser Ile Pro Gly Thr Pro Thr Val Asp 
    770                 775                 780                 


Leu Gly Thr Ser Gly Thr Pro Val Ser Lys Pro Gly Pro Ser Ala Ala 
785                 790                 795                 800 


Ser His Leu Leu Ile Leu Phe Thr Leu Asn Phe Thr Ile Thr Asn Leu 
                805                 810                 815     


Arg Tyr Glu Glu Asn Met Trp Pro Gly Ser Arg Lys Phe Asn Thr Thr 
            820                 825                 830         


Glu Arg Val Leu Gln Gly Leu Leu Arg Pro Leu Phe Lys Asn Thr Ser 
        835                 840                 845             


Val Gly Pro Leu Tyr Ser Gly Ser Arg Leu Thr Leu Leu Arg Pro Glu 
    850                 855                 860                 


Lys Asp Gly Glu Ala Thr Gly Val Asp Ala Ile Cys Thr His Arg Pro 
865                 870                 875                 880 


Asp Pro Thr Gly Pro Gly Leu Asp Arg Glu Gln Leu Tyr Leu Glu Leu 
                885                 890                 895     


Ser Gln Leu Thr His Ser Ile Thr Glu Leu Gly Pro Tyr Thr Leu Asp 
            900                 905                 910         


Arg Asp Ser Leu Tyr Val Asn Gly Phe Thr His Arg Ser Ser Val Pro 
        915                 920                 925             


Thr Thr Ser Thr Gly Val Val Ser Glu Glu Pro Phe Thr Leu Asn Phe 
    930                 935                 940                 


Thr Ile Asn Asn Leu Arg Tyr Met Ala Asp Met Gly Gln Pro Gly Ser 
945                 950                 955                 960 


Leu Lys Phe Asn Ile Thr Asp Asn Val Met Lys His Leu Leu Ser Pro 
                965                 970                 975     


Leu Phe Gln Arg Ser Ser Leu Gly Ala Arg Tyr Thr Gly Cys Arg Val 
            980                 985                 990         


Ile Ala Leu Arg Ser Val Lys Asn  Gly Ala Glu Thr Arg  Val Asp Leu 
        995                 1000                 1005             


Leu Cys  Thr Tyr Leu Gln Pro  Leu Ser Gly Pro Gly  Leu Pro Ile 
    1010                 1015                 1020             


Lys Gln  Val Phe His Glu Leu  Ser Gln Gln Thr His  Gly Ile Thr 
    1025                 1030                 1035             


Arg Leu  Gly Pro Tyr Ser Leu  Asp Lys Asp Ser Leu  Tyr Leu Asn 
    1040                 1045                 1050             


Gly Tyr  Asn Glu Pro Gly Leu  Asp Glu Pro Pro Thr  Thr Pro Lys 
    1055                 1060                 1065             


Pro Ala  Thr Thr Phe Leu Pro  Pro Leu Ser Glu Ala  Thr Thr Ala 
    1070                 1075                 1080             


Met Gly  Tyr His Leu Lys Thr  Leu Thr Leu Asn Phe  Thr Ile Ser 
    1085                 1090                 1095             


Asn Leu  Gln Tyr Ser Pro Asp  Met Gly Lys Gly Ser  Ala Thr Phe 
    1100                 1105                 1110             


Asn Ser  Thr Glu Gly Val Leu  Gln His Leu Leu Arg  Pro Leu Phe 
    1115                 1120                 1125             


Gln Lys  Ser Ser Met Gly Pro  Phe Tyr Leu Gly Cys  Gln Leu Ile 
    1130                 1135                 1140             


Ser Leu  Arg Pro Glu Lys Asp  Gly Ala Ala Thr Gly  Val Asp Thr 
    1145                 1150                 1155             


Thr Cys  Thr Tyr His Pro Asp  Pro Val Gly Pro Gly  Leu Asp Ile 
    1160                 1165                 1170             


Gln Gln  Leu Tyr Trp Glu Leu  Ser Gln Leu Thr His  Gly Val Thr 
    1175                 1180                 1185             


Gln Leu  Gly Phe Tyr Val Leu  Asp Arg Asp Ser Leu  Phe Ile Asn 
    1190                 1195                 1200             


Gly Tyr  Ala Pro Gln Asn Leu  Ser Ile Arg Gly Glu  Tyr Gln Ile 
    1205                 1210                 1215             


Asn Phe  His Ile Val Asn Trp  Asn Leu Ser Asn Pro  Asp Pro Thr 
    1220                 1225                 1230             


Ser Ser  Glu Tyr Ile Ala Leu  Leu Arg Asp Ile Gln  Asp Lys Val 
    1235                 1240                 1245             


Thr Thr  Leu Tyr Lys Gly Ser  Gln Leu His Asp Thr  Phe Arg Phe 
    1250                 1255                 1260             


Cys Leu  Val Thr Asn Leu Thr  Met Asp Ser Met Leu  Val Thr Val 
    1265                 1270                 1275             


Lys Ala  Leu Phe Ser Ser Asn  Leu Asp Pro Ser Leu  Val Glu Gln 
    1280                 1285                 1290             


Val Phe  Leu Asp Lys Thr Leu  Asn Ala Ser Ser His  Trp Leu Gly 
    1295                 1300                 1305             


Ser Thr  Tyr Gln Leu Val Asp  Ile His Val Thr Glu  Met Glu Pro 
    1310                 1315                 1320             


Ser Val  Tyr Gln Pro Thr Ser  Ser Ser Ser Thr Gln  His Phe Tyr 
    1325                 1330                 1335             


Leu Asn  Phe Thr Ile Thr Asn  Leu Pro Tyr Ser Gln  Asp Ile Ala 
    1340                 1345                 1350             


Gln Pro  Gly Thr Thr Asn Tyr  Gln Arg Asn Lys Arg  Asn Ile Glu 
    1355                 1360                 1365             


Asp Ala  Leu Asn Gln Leu Phe  Arg Asn Ser Ser Ile  Lys Ser Tyr 
    1370                 1375                 1380             


Phe Ser  Asp Cys Gln Val Ser  Thr Phe Arg Ser Val  Pro Asn Ser 
    1385                 1390                 1395             


His His  Thr Gly Val Asp Ser  Leu Cys Ala Phe Ser  Pro Leu Ala 
    1400                 1405                 1410             


Arg Arg  Val Asp Arg Val Ala  Ile Tyr Glu Glu Phe  Leu Arg Met 
    1415                 1420                 1425             


Thr Arg  Ala Gly Thr Gln Leu  Gln Ala Phe Thr Leu  Asp Arg Ser 
    1430                 1435                 1440             


Ser Val  Leu Val Asp Gly Tyr  Ser Pro Asn Arg Asn  Glu Pro Leu 
    1445                 1450                 1455             


Thr Gly  Asn Ser Asp Leu Pro  Phe Trp Ala Ile Ile  Leu Ile Cys 
    1460                 1465                 1470             


Leu Ala  Gly Leu Leu Gly Leu  Ile Thr Cys Leu Ile  Cys Gly Phe 
    1475                 1480                 1485             


Leu Val  
    1490 


<210>  3
<211>  1932
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic consensus MUC16 RMC DNA Coding Sequence

<400>  3
atggattgga cttggattct gttcctggtc gccgccgcaa ctcgggtgca ttctactgct       60

gctgggccac tgctggtgcc ttttacactg aacttcacca tcacaaatct gcagtacgag      120

gaggacatgc accaccctgg ctctcggaag ttcaacacca cagagagagt gctgcagggc      180

ctgctgggcc caatgtttaa gaataccagc gtgggcctgc tgtattccgg atgccggctg      240

acactgctga gatccgagaa ggacggagca gcaaccggag tggatgccat ctgtacacac      300

aggctggacc caaagtcccc aggcctggat agagagcagc tgtactggga gctgtctcag      360

ctgaccaacg gcatcaagga gctgggcccc tacacactgg accggaacag cctgtatgtg      420

aatggcttta cccacagaag ctccgtgcca aataccagca cacccggcac ctccacagtg      480

gatctgggca cctctggcac accttctagc ctgccaagcc ctaccgcagc aggaccactg      540

ctggtgccct tcacactgaa ctttaccatt accaatctgc agtatgaaga ggacatgagg      600

cacccaggct cccgcaagtt caacactacc gagcgggtgc tgcaaggcct gctgaagcct      660

ctgtttaaga atacctctgt gggcccactg tatagtggct gccggctgac actgctgcgg      720

cccgaaaaag acggagcagc aaccggcgtg gatgctattt gcacccacag gctggacccc      780

aagagcccag gcctgaaccg cgaacagctg tattgggagc tgtccaagct gaccaatggc      840

atcacagagc tgggccccta caccctggac agaaattccc tgtacgtgaa tggcttcacc      900

caccgcacat ctgtgcctac cacaagcacc ccaggcacct ccacagtgga tctgggcacc      960

tccggcacac ccttttccct gccatctcca accacagcag gacctctgct ggtgccattc     1020

accctgaact ttaccattac taatctgcag tatgaagaag acatgcacag gcctggctct     1080

cgcaagttca acactactga gagggtgctg cagggcctgt taagcccaat ctttaagaat     1140

tcctctgtgg gccctctgta ttccggatgc aggctgacct ctctgcgccc agaaaaagat     1200

ggagcagcaa caggaatgga tgccgtgtgc ctgtaccacc ctaacccaaa gcggcccggc     1260

ctggacaggg agcagctgta ttgggaactg agccagctga cccacaatat cacagagctg     1320

ggcccttact ctctggaccg cgatagcctg tatgtgaacg gcttcaccca ccagaattcc     1380

gtgcccacca catctacacc tggcaccagc acagtgtact gggccaccac aggcaccccc     1440

agctcctttc ctggacacac agcaccagga cctctgctga tccccttcac cctgaacttt     1500

accataacaa atctgcacta tgaggagaac atgcagcacc ctggcagcag gaagttcaat     1560

accacagagc gcgtgctgca aggcctgctg aagccgctgt ttaagaacac cagcgtggga     1620

ccactgtaca gcggctgcag gctgaccctg ctgcgccctg agaagcatgg cgccgccacc     1680

ggcgtggatg ctatctgcac acatagactg gaccccaagg gacctggcct ggatagggag     1740

agactgtact gggaactgtc ccagctgacc aactcaatta cagagctggg cccatacacc     1800

ctggaccggg attctctgta cgtgaacggc ttcaatccaa gatctagcgt ccctaccaca     1860

tctacccctg ggacaagtac cgtgcatctg gctacaagcg gaactccttc aagtctgcct     1920

ggacactgat aa                                                         1932


<210>  4
<211>  642
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic consensus MUC16 RMC Protein Sequence

<400>  4

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Thr Ala Ala Gly Pro Leu Leu Val Pro Phe Thr Leu Asn Phe 
            20                  25                  30          


Thr Ile Thr Asn Leu Gln Tyr Glu Glu Asp Met His His Pro Gly Ser 
        35                  40                  45              


Arg Lys Phe Asn Thr Thr Glu Arg Val Leu Gln Gly Leu Leu Gly Pro 
    50                  55                  60                  


Met Phe Lys Asn Thr Ser Val Gly Leu Leu Tyr Ser Gly Cys Arg Leu 
65                  70                  75                  80  


Thr Leu Leu Arg Ser Glu Lys Asp Gly Ala Ala Thr Gly Val Asp Ala 
                85                  90                  95      


Ile Cys Thr His Arg Leu Asp Pro Lys Ser Pro Gly Leu Asp Arg Glu 
            100                 105                 110         


Gln Leu Tyr Trp Glu Leu Ser Gln Leu Thr Asn Gly Ile Lys Glu Leu 
        115                 120                 125             


Gly Pro Tyr Thr Leu Asp Arg Asn Ser Leu Tyr Val Asn Gly Phe Thr 
    130                 135                 140                 


His Arg Ser Ser Val Pro Asn Thr Ser Thr Pro Gly Thr Ser Thr Val 
145                 150                 155                 160 


Asp Leu Gly Thr Ser Gly Thr Pro Ser Ser Leu Pro Ser Pro Thr Ala 
                165                 170                 175     


Ala Gly Pro Leu Leu Val Pro Phe Thr Leu Asn Phe Thr Ile Thr Asn 
            180                 185                 190         


Leu Gln Tyr Glu Glu Asp Met Arg His Pro Gly Ser Arg Lys Phe Asn 
        195                 200                 205             


Thr Thr Glu Arg Val Leu Gln Gly Leu Leu Lys Pro Leu Phe Lys Asn 
    210                 215                 220                 


Thr Ser Val Gly Pro Leu Tyr Ser Gly Cys Arg Leu Thr Leu Leu Arg 
225                 230                 235                 240 


Pro Glu Lys Asp Gly Ala Ala Thr Gly Val Asp Ala Ile Cys Thr His 
                245                 250                 255     


Arg Leu Asp Pro Lys Ser Pro Gly Leu Asn Arg Glu Gln Leu Tyr Trp 
            260                 265                 270         


Glu Leu Ser Lys Leu Thr Asn Gly Ile Thr Glu Leu Gly Pro Tyr Thr 
        275                 280                 285             


Leu Asp Arg Asn Ser Leu Tyr Val Asn Gly Phe Thr His Arg Thr Ser 
    290                 295                 300                 


Val Pro Thr Thr Ser Thr Pro Gly Thr Ser Thr Val Asp Leu Gly Thr 
305                 310                 315                 320 


Ser Gly Thr Pro Phe Ser Leu Pro Ser Pro Thr Thr Ala Gly Pro Leu 
                325                 330                 335     


Leu Val Pro Phe Thr Leu Asn Phe Thr Ile Thr Asn Leu Gln Tyr Glu 
            340                 345                 350         


Glu Asp Met His Arg Pro Gly Ser Arg Lys Phe Asn Thr Thr Glu Arg 
        355                 360                 365             


Val Leu Gln Gly Leu Leu Ser Pro Ile Phe Lys Asn Ser Ser Val Gly 
    370                 375                 380                 


Pro Leu Tyr Ser Gly Cys Arg Leu Thr Ser Leu Arg Pro Glu Lys Asp 
385                 390                 395                 400 


Gly Ala Ala Thr Gly Met Asp Ala Val Cys Leu Tyr His Pro Asn Pro 
                405                 410                 415     


Lys Arg Pro Gly Leu Asp Arg Glu Gln Leu Tyr Trp Glu Leu Ser Gln 
            420                 425                 430         


Leu Thr His Asn Ile Thr Glu Leu Gly Pro Tyr Ser Leu Asp Arg Asp 
        435                 440                 445             


Ser Leu Tyr Val Asn Gly Phe Thr His Gln Asn Ser Val Pro Thr Thr 
    450                 455                 460                 


Ser Thr Pro Gly Thr Ser Thr Val Tyr Trp Ala Thr Thr Gly Thr Pro 
465                 470                 475                 480 


Ser Ser Phe Pro Gly His Thr Ala Pro Gly Pro Leu Leu Ile Pro Phe 
                485                 490                 495     


Thr Leu Asn Phe Thr Ile Thr Asn Leu His Tyr Glu Glu Asn Met Gln 
            500                 505                 510         


His Pro Gly Ser Arg Lys Phe Asn Thr Thr Glu Arg Val Leu Gln Gly 
        515                 520                 525             


Leu Leu Lys Pro Leu Phe Lys Asn Thr Ser Val Gly Pro Leu Tyr Ser 
    530                 535                 540                 


Gly Cys Arg Leu Thr Leu Leu Arg Pro Glu Lys His Gly Ala Ala Thr 
545                 550                 555                 560 


Gly Val Asp Ala Ile Cys Thr His Arg Leu Asp Pro Lys Gly Pro Gly 
                565                 570                 575     


Leu Asp Arg Glu Arg Leu Tyr Trp Glu Leu Ser Gln Leu Thr Asn Ser 
            580                 585                 590         


Ile Thr Glu Leu Gly Pro Tyr Thr Leu Asp Arg Asp Ser Leu Tyr Val 
        595                 600                 605             


Asn Gly Phe Asn Pro Arg Ser Ser Val Pro Thr Thr Ser Thr Pro Gly 
    610                 615                 620                 


Thr Ser Thr Val His Leu Ala Thr Ser Gly Thr Pro Ser Ser Leu Pro 
625                 630                 635                 640 


Gly His 
        


<210>  5
<211>  2136
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic consensus MUC16 NRC DNA Coding Sequence

<400>  5
atggactgga cttggattct gttcctggtc gctgccgcca cccgcgtgca tagtgccgca       60

tctcacctgc tgattctgtt caccctgaac ttcaccatca caaatctgcg gtacgaggag      120

aacatgtggc caggcagccg caagttcaat accacagagc gggtgctgca gggcctgctg      180

cggcccctgt ttaagaacac ctccgtgggc cccctgtatt ctggcagcag gctgaccctg      240

ctgcgcccag agaaggacgg agaggcaaca ggcgtggatg ccatctgcac ccacaggcct      300

gacccaacag gaccaggcct ggatagggag cagctgtacc tggagctgag ccagctgacc      360

cactccatca cagagctggg accatacacc ctggacaggg attccctgta tgtgaacggc      420

tttacccaca gaagctccgt gcccaccaca tctacaggcg tggtgagcga ggagcccttc      480

accctgaact tcaccatcaa caatctgagg tacatggcag acatgggaca gccaggcagc      540

ctgaagttca acatcaccga taatgtgatg aagcacctgc tgtcccctct gtttcagcgg      600

tctagcctgg gagcaaggta caccggctgc agagtgatcg ccctgaggtc cgtgaagaac      660

ggagcagaga cacgggtgga cctgctgtgc acatatctgc agcctctgag cggaccaggc      720

ctgcctatca agcaggtgtt ccacgagctg tctcagcaga cccacggaat cacacgcctg      780

ggaccttact ccctggacaa ggattctctg tacctgaacg gctataatga gccaggcctg      840

gacgagcccc ctaccacacc caagcctgcc accacatttc tgccacccct gagcgaggca      900

accacagcaa tgggatacca cctgaagacc ctgacactga acttcaccat cagcaatctg      960

cagtattccc ccgatatggg caagggctct gccaccttta atagcacaga gggcgtgctg     1020

cagcacctgc tgaggcctct gttccagaag tcctctatgg gccccttcta cctgggatgc     1080

cagctgatct ccctgcgccc tgagaaggac ggagcagcaa ccggagtgga taccacatgt     1140

acataccacc cagaccccgt gggaccaggc ctggatatcc agcagctgta ttgggaactg     1200

tcccagctga cccacggcgt gacacagctg ggcttctatg tgctggaccg ggattctctg     1260

tttatcaacg gctacgcccc tcagaatctg agcatcagag gcgagtatca gatcaacttc     1320

cacatcgtga actggaatct gtctaatcct gatccaacca gctccgagta catcgccctg     1380

ctgcgggaca tccaggataa ggtgaccaca ctgtataagg gcagccagct gcacgacacc     1440

ttcagatttt gtctggtgac caacctgaca atggattcca tgctggtgac agtgaaggcc     1500

ctgttctcta gcaacctgga cccttctctg gtggagcagg tgtttctgga taagaccctg     1560

aatgcctcct ctcactggct gggctctacc taccagctgg tggacatcca cgtgacagag     1620

atggagccaa gcgtgtatca gcccaccagc tcctctagca cacagcactt ctacctgaac     1680

tttaccatca caaatctgcc ctatagccag gatatcgccc agcctggcac cacaaactac     1740

cagaggaaca agcgcaatat cgaggacgcc ctgaaccagc tgttcaggaa ttcctctatc     1800

aagtcctatt tctctgattg ccaggtgagc acctttcgct ccgtgccaaa ttctcaccac     1860

acaggcgtgg actccctgtg cgccttttct cccctggcac ggagagtgga tagggtggca     1920

atctacgagg agttcctgcg gatgaccaga gccggcacac agctgcaggc ctttaccctg     1980

gacaggagct ccgtgctggt ggatggctat agccctaacc gcaatgagcc actgacaggc     2040

aattccgacc tgcccttctg ggccatcatc ctgatttgcc tggctggact gctggggctg     2100

attacctgtc tgatttgtgg gttcctggtg tgataa                               2136


<210>  6
<211>  710
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic consensus MUC16 NRC Protein Sequence

<400>  6

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Ala Ala Ser His Leu Leu Ile Leu Phe Thr Leu Asn Phe Thr 
            20                  25                  30          


Ile Thr Asn Leu Arg Tyr Glu Glu Asn Met Trp Pro Gly Ser Arg Lys 
        35                  40                  45              


Phe Asn Thr Thr Glu Arg Val Leu Gln Gly Leu Leu Arg Pro Leu Phe 
    50                  55                  60                  


Lys Asn Thr Ser Val Gly Pro Leu Tyr Ser Gly Ser Arg Leu Thr Leu 
65                  70                  75                  80  


Leu Arg Pro Glu Lys Asp Gly Glu Ala Thr Gly Val Asp Ala Ile Cys 
                85                  90                  95      


Thr His Arg Pro Asp Pro Thr Gly Pro Gly Leu Asp Arg Glu Gln Leu 
            100                 105                 110         


Tyr Leu Glu Leu Ser Gln Leu Thr His Ser Ile Thr Glu Leu Gly Pro 
        115                 120                 125             


Tyr Thr Leu Asp Arg Asp Ser Leu Tyr Val Asn Gly Phe Thr His Arg 
    130                 135                 140                 


Ser Ser Val Pro Thr Thr Ser Thr Gly Val Val Ser Glu Glu Pro Phe 
145                 150                 155                 160 


Thr Leu Asn Phe Thr Ile Asn Asn Leu Arg Tyr Met Ala Asp Met Gly 
                165                 170                 175     


Gln Pro Gly Ser Leu Lys Phe Asn Ile Thr Asp Asn Val Met Lys His 
            180                 185                 190         


Leu Leu Ser Pro Leu Phe Gln Arg Ser Ser Leu Gly Ala Arg Tyr Thr 
        195                 200                 205             


Gly Cys Arg Val Ile Ala Leu Arg Ser Val Lys Asn Gly Ala Glu Thr 
    210                 215                 220                 


Arg Val Asp Leu Leu Cys Thr Tyr Leu Gln Pro Leu Ser Gly Pro Gly 
225                 230                 235                 240 


Leu Pro Ile Lys Gln Val Phe His Glu Leu Ser Gln Gln Thr His Gly 
                245                 250                 255     


Ile Thr Arg Leu Gly Pro Tyr Ser Leu Asp Lys Asp Ser Leu Tyr Leu 
            260                 265                 270         


Asn Gly Tyr Asn Glu Pro Gly Leu Asp Glu Pro Pro Thr Thr Pro Lys 
        275                 280                 285             


Pro Ala Thr Thr Phe Leu Pro Pro Leu Ser Glu Ala Thr Thr Ala Met 
    290                 295                 300                 


Gly Tyr His Leu Lys Thr Leu Thr Leu Asn Phe Thr Ile Ser Asn Leu 
305                 310                 315                 320 


Gln Tyr Ser Pro Asp Met Gly Lys Gly Ser Ala Thr Phe Asn Ser Thr 
                325                 330                 335     


Glu Gly Val Leu Gln His Leu Leu Arg Pro Leu Phe Gln Lys Ser Ser 
            340                 345                 350         


Met Gly Pro Phe Tyr Leu Gly Cys Gln Leu Ile Ser Leu Arg Pro Glu 
        355                 360                 365             


Lys Asp Gly Ala Ala Thr Gly Val Asp Thr Thr Cys Thr Tyr His Pro 
    370                 375                 380                 


Asp Pro Val Gly Pro Gly Leu Asp Ile Gln Gln Leu Tyr Trp Glu Leu 
385                 390                 395                 400 


Ser Gln Leu Thr His Gly Val Thr Gln Leu Gly Phe Tyr Val Leu Asp 
                405                 410                 415     


Arg Asp Ser Leu Phe Ile Asn Gly Tyr Ala Pro Gln Asn Leu Ser Ile 
            420                 425                 430         


Arg Gly Glu Tyr Gln Ile Asn Phe His Ile Val Asn Trp Asn Leu Ser 
        435                 440                 445             


Asn Pro Asp Pro Thr Ser Ser Glu Tyr Ile Ala Leu Leu Arg Asp Ile 
    450                 455                 460                 


Gln Asp Lys Val Thr Thr Leu Tyr Lys Gly Ser Gln Leu His Asp Thr 
465                 470                 475                 480 


Phe Arg Phe Cys Leu Val Thr Asn Leu Thr Met Asp Ser Met Leu Val 
                485                 490                 495     


Thr Val Lys Ala Leu Phe Ser Ser Asn Leu Asp Pro Ser Leu Val Glu 
            500                 505                 510         


Gln Val Phe Leu Asp Lys Thr Leu Asn Ala Ser Ser His Trp Leu Gly 
        515                 520                 525             


Ser Thr Tyr Gln Leu Val Asp Ile His Val Thr Glu Met Glu Pro Ser 
    530                 535                 540                 


Val Tyr Gln Pro Thr Ser Ser Ser Ser Thr Gln His Phe Tyr Leu Asn 
545                 550                 555                 560 


Phe Thr Ile Thr Asn Leu Pro Tyr Ser Gln Asp Ile Ala Gln Pro Gly 
                565                 570                 575     


Thr Thr Asn Tyr Gln Arg Asn Lys Arg Asn Ile Glu Asp Ala Leu Asn 
            580                 585                 590         


Gln Leu Phe Arg Asn Ser Ser Ile Lys Ser Tyr Phe Ser Asp Cys Gln 
        595                 600                 605             


Val Ser Thr Phe Arg Ser Val Pro Asn Ser His His Thr Gly Val Asp 
    610                 615                 620                 


Ser Leu Cys Ala Phe Ser Pro Leu Ala Arg Arg Val Asp Arg Val Ala 
625                 630                 635                 640 


Ile Tyr Glu Glu Phe Leu Arg Met Thr Arg Ala Gly Thr Gln Leu Gln 
                645                 650                 655     


Ala Phe Thr Leu Asp Arg Ser Ser Val Leu Val Asp Gly Tyr Ser Pro 
            660                 665                 670         


Asn Arg Asn Glu Pro Leu Thr Gly Asn Ser Asp Leu Pro Phe Trp Ala 
        675                 680                 685             


Ile Ile Leu Ile Cys Leu Ala Gly Leu Leu Gly Leu Ile Thr Cys Leu 
    690                 695                 700                 


Ile Cys Gly Phe Leu Val 
705                 710 


<210>  7
<211>  4029
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic consensus MUC16 IRC DNA Coding Sequence

<400>  7
atggactgga cctggattct gttcctggtg gcagcagcaa ccagggtgca ctccacagca       60

gcaggacctc tgctggtgcc attcaccctg aactttacca tcacaaatct gcagtacgag      120

gaggatatgc accaccccgg cagccgcaag ttcaacacca cagagcgggt gctgcagggc      180

ctgctgggac ctatgtttaa gaataccagc gtgggcctgc tgtattccgg atgcaggctg      240

acactgctgc gctccgagaa ggacggagca gcaaccggcg tggatgccat ctgtacacac      300

aggctggacc caaagtctcc cggcctggat cgcgagcagc tgtactggga gctgagccag      360

ctgaccaacg gcatcaagga gctgggcccc tacacactgg accggaacag cctgtatgtg      420

aatggcttca cccacagaag ctccgtgcca aatacctcca cacccggcac ctctacagtg      480

gatctgggca cctctggcac accctctagc ctgcctagcc caaccgcagc aggaccactg      540

ctggtgcctt tcacactgaa ctttaccatt accaatctgc agtatgaaga ggacatgcgg      600

caccctggca gcagaaagtt caacactacc gagcgcgtgc tgcaaggcct gctgaagcca      660

ctgtttaaga atacctctgt gggccccctg tatagtggct gtagactgac actgctgcgc      720

cctgaaaaag atggcgccgc cactggagtc gacgctattt gcacccacag gctggacccc      780

aagtccccag gcctgaacag agaacagctg tattgggagc tgtctaagct gaccaatggc      840

atcacagagc tgggcccata caccctggac aggaactctc tgtacgtcaa tggcttcacc      900

caccgcacaa gcgtgcctac cacatccacc ccaggcacct ctaccgtcga tctgggcacc      960

agcggcacac cattttccct gccatctcct accaccgccg gaccactgct ggtgcccttc     1020

accctgaact ttaccataac caatctgcag tatgaggagg acatgcaccg gcccggctct     1080

agaaagttca acactactga acgggtgctg caaggcctgt taagccctat ctttaagaat     1140

tcctctgtgg gcccactgta cagcggatgc aggctgacct ctctgcggcc cgaaaaagac     1200

ggagcagcaa caggaatgga tgccgtgtgc ctgtaccacc caaaccccaa gaggcctggc     1260

ctggacagag agcagctgta ttgggaactg tcccagctga cccacaatat cacagagctg     1320

ggcccctaca gcctggacag agattccctg tatgtgaacg gcttcaccca ccagaattct     1380

gtgcccacca caagcacacc tggcacctcc acagtgtact gggccaccac aggcacccct     1440

agctcctttc caggacacac agcacctgga ccactgctga tccctttcac cctgaacttt     1500

accattacaa atctgcacta tgaggagaac atgcagcacc caggcagcag aaagttcaat     1560

accacagaga gggtgctgca aggcctgctg aagccgctgt ttaagaacac cagcgtggga     1620

cctctgtact ctggctgtcg cctgacactg ctgcggcccg agaagcatgg cgcagcaacc     1680

ggcgtggacg ctatttgcac tcatagactg gaccccaagg gacctggcct ggatagggag     1740

agactgtact gggaactgtc tcagctgacc aattccatta cagagctggg cccttacacc     1800

ctggaccggg acagcctgta cgtcaacggc ttcaatccaa gatctagcgt gcccaccaca     1860

tccacccctg gcacctctac agtgcacctg gccaccagcg gaacaccctc ctctctgcct     1920

ggacacaggg gaaggaagcg gagaagcgcc gcatcccacc tgctgatcct gttcaccctg     1980

aactttacca taacgaatct gagatacgag gagaacatgt ggcctggctc ccgcaagttc     2040

aatactaccg aacgggtgct gcagggcctg ctgcggcccc tgtttaagaa cacttccgtg     2100

ggccccctgt attctggcag caggctgacc ctgctgcgcc cagagaagga cggagaggca     2160

acaggcgtgg atgccatctg cacccacagg cccgacccta caggaccagg cctggatagg     2220

gagcagctgt acctggagct gtcccagctg actcactcaa ttaccgaact gggaccttac     2280

accctggaca gggatagtct gtacgtgaat ggcttcaccc atcgcagctc cgtgccaacc     2340

acatctacag gcgtggtgag cgaggaaccc ttcaccctga acttcaccat caacaatctg     2400

aggtacatgg ccgacatggg ccagccaggc tccctgaagt tcaacatcac cgataatgtg     2460

atgaagcacc tgctgtctcc cctgtttcag aggtctagcc tgggagcaag gtacaccgga     2520

tgcagagtga tcgccctgag gtccgtgaag aacggagcag agacacgggt ggacctgctg     2580

tgcacatatc tgcagcctct gagcggacca ggcctgccca tcaagcaggt gttccacgag     2640

ctgtcccagc agacccacgg aatcacaagg ctgggacctt actccctgga caaggattct     2700

ctgtacctga acggctataa tgagccaggc ctggacgagc cccctaccac accaaagccc     2760

gccaccacat ttctgccacc cctgagcgag gcaaccacag caatgggata ccacctgaag     2820

accctgacac tgaacttcac catcagcaat ctgcagtatt cccccgatat gggcaagggc     2880

tctgccacct ttaacagcac agagggcgtg ctgcagcacc tgctgcggcc tctgttccag     2940

aagtcctcta tgggcccctt ctacctggga tgccagctga tctccctgcg gcccgaaaag     3000

gatggagcag caaccggagt ggataccaca tgtacatacc accctgaccc agtgggacca     3060

ggcctggata tccagcaatt atattgggaa ctgagtcagc tgacccacgg cgtgacacag     3120

ctgggcttct atgtgctgga cagggatagc ctgtttatca acggctacgc cccacagaat     3180

ctgtccatcc gcggcgagta tcagatcaac ttccacatcg tgaactggaa tctgagcaat     3240

cccgacccta ccagctccga gtacatcgcc ctgctgaggg acatccagga taaggtgacc     3300

acactgtata agggctccca gctgcacgac accttccgct tttgcctggt gaccaacctg     3360

acaatggatt ctatgctggt gacagtgaag gccctgttct ctagcaacct ggaccccagc     3420

ctggtggagc aggtgtttct ggataagacc ctgaatgcct cctctcactg gctgggcagc     3480

acctaccagc tggtggacat ccacgtgaca gagatggagc catccgtgta tcagcccacc     3540

agctcctcta gcacacagca cttctacctg aactttacca taactaatct gccctatagc     3600

caggatatcg cccagcctgg caccacaaac taccagcgga acaagagaaa tatcgaggac     3660

gccctgaacc agctgttccg gaattcctct atcaagtctt atttcagcga ttgccaggtg     3720

tccaccttta gatctgtgcc aaatagccac cacacaggcg tggactccct gtgcgccttt     3780

tctcccctgg caaggagggt ggatagggtg gcaatctacg aggagttcct gaggatgacc     3840

cgcgccggaa cacagctgca ggcctttacc ctggaccgga gctccgtgct ggtggatggc     3900

tattccccta acagaaatga gccactgaca ggcaactctg acctgccctt ctgggccatc     3960

atcctgatct gtctggcagg cctgctgggc ctgatcacct gcctgatctg tggctttctg     4020

gtgtgataa                                                             4029


<210>  8
<211>  1341
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic consensus MUC16 IRC Protein Sequence

<400>  8

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Thr Ala Ala Gly Pro Leu Leu Val Pro Phe Thr Leu Asn Phe 
            20                  25                  30          


Thr Ile Thr Asn Leu Gln Tyr Glu Glu Asp Met His His Pro Gly Ser 
        35                  40                  45              


Arg Lys Phe Asn Thr Thr Glu Arg Val Leu Gln Gly Leu Leu Gly Pro 
    50                  55                  60                  


Met Phe Lys Asn Thr Ser Val Gly Leu Leu Tyr Ser Gly Cys Arg Leu 
65                  70                  75                  80  


Thr Leu Leu Arg Ser Glu Lys Asp Gly Ala Ala Thr Gly Val Asp Ala 
                85                  90                  95      


Ile Cys Thr His Arg Leu Asp Pro Lys Ser Pro Gly Leu Asp Arg Glu 
            100                 105                 110         


Gln Leu Tyr Trp Glu Leu Ser Gln Leu Thr Asn Gly Ile Lys Glu Leu 
        115                 120                 125             


Gly Pro Tyr Thr Leu Asp Arg Asn Ser Leu Tyr Val Asn Gly Phe Thr 
    130                 135                 140                 


His Arg Ser Ser Val Pro Asn Thr Ser Thr Pro Gly Thr Ser Thr Val 
145                 150                 155                 160 


Asp Leu Gly Thr Ser Gly Thr Pro Ser Ser Leu Pro Ser Pro Thr Ala 
                165                 170                 175     


Ala Gly Pro Leu Leu Val Pro Phe Thr Leu Asn Phe Thr Ile Thr Asn 
            180                 185                 190         


Leu Gln Tyr Glu Glu Asp Met Arg His Pro Gly Ser Arg Lys Phe Asn 
        195                 200                 205             


Thr Thr Glu Arg Val Leu Gln Gly Leu Leu Lys Pro Leu Phe Lys Asn 
    210                 215                 220                 


Thr Ser Val Gly Pro Leu Tyr Ser Gly Cys Arg Leu Thr Leu Leu Arg 
225                 230                 235                 240 


Pro Glu Lys Asp Gly Ala Ala Thr Gly Val Asp Ala Ile Cys Thr His 
                245                 250                 255     


Arg Leu Asp Pro Lys Ser Pro Gly Leu Asn Arg Glu Gln Leu Tyr Trp 
            260                 265                 270         


Glu Leu Ser Lys Leu Thr Asn Gly Ile Thr Glu Leu Gly Pro Tyr Thr 
        275                 280                 285             


Leu Asp Arg Asn Ser Leu Tyr Val Asn Gly Phe Thr His Arg Thr Ser 
    290                 295                 300                 


Val Pro Thr Thr Ser Thr Pro Gly Thr Ser Thr Val Asp Leu Gly Thr 
305                 310                 315                 320 


Ser Gly Thr Pro Phe Ser Leu Pro Ser Pro Thr Thr Ala Gly Pro Leu 
                325                 330                 335     


Leu Val Pro Phe Thr Leu Asn Phe Thr Ile Thr Asn Leu Gln Tyr Glu 
            340                 345                 350         


Glu Asp Met His Arg Pro Gly Ser Arg Lys Phe Asn Thr Thr Glu Arg 
        355                 360                 365             


Val Leu Gln Gly Leu Leu Ser Pro Ile Phe Lys Asn Ser Ser Val Gly 
    370                 375                 380                 


Pro Leu Tyr Ser Gly Cys Arg Leu Thr Ser Leu Arg Pro Glu Lys Asp 
385                 390                 395                 400 


Gly Ala Ala Thr Gly Met Asp Ala Val Cys Leu Tyr His Pro Asn Pro 
                405                 410                 415     


Lys Arg Pro Gly Leu Asp Arg Glu Gln Leu Tyr Trp Glu Leu Ser Gln 
            420                 425                 430         


Leu Thr His Asn Ile Thr Glu Leu Gly Pro Tyr Ser Leu Asp Arg Asp 
        435                 440                 445             


Ser Leu Tyr Val Asn Gly Phe Thr His Gln Asn Ser Val Pro Thr Thr 
    450                 455                 460                 


Ser Thr Pro Gly Thr Ser Thr Val Tyr Trp Ala Thr Thr Gly Thr Pro 
465                 470                 475                 480 


Ser Ser Phe Pro Gly His Thr Ala Pro Gly Pro Leu Leu Ile Pro Phe 
                485                 490                 495     


Thr Leu Asn Phe Thr Ile Thr Asn Leu His Tyr Glu Glu Asn Met Gln 
            500                 505                 510         


His Pro Gly Ser Arg Lys Phe Asn Thr Thr Glu Arg Val Leu Gln Gly 
        515                 520                 525             


Leu Leu Lys Pro Leu Phe Lys Asn Thr Ser Val Gly Pro Leu Tyr Ser 
    530                 535                 540                 


Gly Cys Arg Leu Thr Leu Leu Arg Pro Glu Lys His Gly Ala Ala Thr 
545                 550                 555                 560 


Gly Val Asp Ala Ile Cys Thr His Arg Leu Asp Pro Lys Gly Pro Gly 
                565                 570                 575     


Leu Asp Arg Glu Arg Leu Tyr Trp Glu Leu Ser Gln Leu Thr Asn Ser 
            580                 585                 590         


Ile Thr Glu Leu Gly Pro Tyr Thr Leu Asp Arg Asp Ser Leu Tyr Val 
        595                 600                 605             


Asn Gly Phe Asn Pro Arg Ser Ser Val Pro Thr Thr Ser Thr Pro Gly 
    610                 615                 620                 


Thr Ser Thr Val His Leu Ala Thr Ser Gly Thr Pro Ser Ser Leu Pro 
625                 630                 635                 640 


Gly His Arg Gly Arg Lys Arg Arg Ser Ala Ala Ser His Leu Leu Ile 
                645                 650                 655     


Leu Phe Thr Leu Asn Phe Thr Ile Thr Asn Leu Arg Tyr Glu Glu Asn 
            660                 665                 670         


Met Trp Pro Gly Ser Arg Lys Phe Asn Thr Thr Glu Arg Val Leu Gln 
        675                 680                 685             


Gly Leu Leu Arg Pro Leu Phe Lys Asn Thr Ser Val Gly Pro Leu Tyr 
    690                 695                 700                 


Ser Gly Ser Arg Leu Thr Leu Leu Arg Pro Glu Lys Asp Gly Glu Ala 
705                 710                 715                 720 


Thr Gly Val Asp Ala Ile Cys Thr His Arg Pro Asp Pro Thr Gly Pro 
                725                 730                 735     


Gly Leu Asp Arg Glu Gln Leu Tyr Leu Glu Leu Ser Gln Leu Thr His 
            740                 745                 750         


Ser Ile Thr Glu Leu Gly Pro Tyr Thr Leu Asp Arg Asp Ser Leu Tyr 
        755                 760                 765             


Val Asn Gly Phe Thr His Arg Ser Ser Val Pro Thr Thr Ser Thr Gly 
    770                 775                 780                 


Val Val Ser Glu Glu Pro Phe Thr Leu Asn Phe Thr Ile Asn Asn Leu 
785                 790                 795                 800 


Arg Tyr Met Ala Asp Met Gly Gln Pro Gly Ser Leu Lys Phe Asn Ile 
                805                 810                 815     


Thr Asp Asn Val Met Lys His Leu Leu Ser Pro Leu Phe Gln Arg Ser 
            820                 825                 830         


Ser Leu Gly Ala Arg Tyr Thr Gly Cys Arg Val Ile Ala Leu Arg Ser 
        835                 840                 845             


Val Lys Asn Gly Ala Glu Thr Arg Val Asp Leu Leu Cys Thr Tyr Leu 
    850                 855                 860                 


Gln Pro Leu Ser Gly Pro Gly Leu Pro Ile Lys Gln Val Phe His Glu 
865                 870                 875                 880 


Leu Ser Gln Gln Thr His Gly Ile Thr Arg Leu Gly Pro Tyr Ser Leu 
                885                 890                 895     


Asp Lys Asp Ser Leu Tyr Leu Asn Gly Tyr Asn Glu Pro Gly Leu Asp 
            900                 905                 910         


Glu Pro Pro Thr Thr Pro Lys Pro Ala Thr Thr Phe Leu Pro Pro Leu 
        915                 920                 925             


Ser Glu Ala Thr Thr Ala Met Gly Tyr His Leu Lys Thr Leu Thr Leu 
    930                 935                 940                 


Asn Phe Thr Ile Ser Asn Leu Gln Tyr Ser Pro Asp Met Gly Lys Gly 
945                 950                 955                 960 


Ser Ala Thr Phe Asn Ser Thr Glu Gly Val Leu Gln His Leu Leu Arg 
                965                 970                 975     


Pro Leu Phe Gln Lys Ser Ser Met Gly Pro Phe Tyr Leu Gly Cys Gln 
            980                 985                 990         


Leu Ile Ser Leu Arg Pro Glu Lys  Asp Gly Ala Ala Thr  Gly Val Asp 
        995                 1000                 1005             


Thr Thr  Cys Thr Tyr His Pro  Asp Pro Val Gly Pro  Gly Leu Asp 
    1010                 1015                 1020             


Ile Gln  Gln Leu Tyr Trp Glu  Leu Ser Gln Leu Thr  His Gly Val 
    1025                 1030                 1035             


Thr Gln  Leu Gly Phe Tyr Val  Leu Asp Arg Asp Ser  Leu Phe Ile 
    1040                 1045                 1050             


Asn Gly  Tyr Ala Pro Gln Asn  Leu Ser Ile Arg Gly  Glu Tyr Gln 
    1055                 1060                 1065             


Ile Asn  Phe His Ile Val Asn  Trp Asn Leu Ser Asn  Pro Asp Pro 
    1070                 1075                 1080             


Thr Ser  Ser Glu Tyr Ile Ala  Leu Leu Arg Asp Ile  Gln Asp Lys 
    1085                 1090                 1095             


Val Thr  Thr Leu Tyr Lys Gly  Ser Gln Leu His Asp  Thr Phe Arg 
    1100                 1105                 1110             


Phe Cys  Leu Val Thr Asn Leu  Thr Met Asp Ser Met  Leu Val Thr 
    1115                 1120                 1125             


Val Lys  Ala Leu Phe Ser Ser  Asn Leu Asp Pro Ser  Leu Val Glu 
    1130                 1135                 1140             


Gln Val  Phe Leu Asp Lys Thr  Leu Asn Ala Ser Ser  His Trp Leu 
    1145                 1150                 1155             


Gly Ser  Thr Tyr Gln Leu Val  Asp Ile His Val Thr  Glu Met Glu 
    1160                 1165                 1170             


Pro Ser  Val Tyr Gln Pro Thr  Ser Ser Ser Ser Thr  Gln His Phe 
    1175                 1180                 1185             


Tyr Leu  Asn Phe Thr Ile Thr  Asn Leu Pro Tyr Ser  Gln Asp Ile 
    1190                 1195                 1200             


Ala Gln  Pro Gly Thr Thr Asn  Tyr Gln Arg Asn Lys  Arg Asn Ile 
    1205                 1210                 1215             


Glu Asp  Ala Leu Asn Gln Leu  Phe Arg Asn Ser Ser  Ile Lys Ser 
    1220                 1225                 1230             


Tyr Phe  Ser Asp Cys Gln Val  Ser Thr Phe Arg Ser  Val Pro Asn 
    1235                 1240                 1245             


Ser His  His Thr Gly Val Asp  Ser Leu Cys Ala Phe  Ser Pro Leu 
    1250                 1255                 1260             


Ala Arg  Arg Val Asp Arg Val  Ala Ile Tyr Glu Glu  Phe Leu Arg 
    1265                 1270                 1275             


Met Thr  Arg Ala Gly Thr Gln  Leu Gln Ala Phe Thr  Leu Asp Arg 
    1280                 1285                 1290             


Ser Ser  Val Leu Val Asp Gly  Tyr Ser Pro Asn Arg  Asn Glu Pro 
    1295                 1300                 1305             


Leu Thr  Gly Asn Ser Asp Leu  Pro Phe Trp Ala Ile  Ile Leu Ile 
    1310                 1315                 1320             


Cys Leu  Ala Gly Leu Leu Gly  Leu Ile Thr Cys Leu  Ile Cys Gly 
    1325                 1330                 1335             


Phe Leu  Val 
    1340     


