                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       Zhao, Yangbing
       Liu, Xiaojun
 
<120>  GENETICALLY MODIFIED IMMUNE CELLS TARGETING NY-ESO-1 AND METHODS 
       OF USE THEREOF

<130>  046483-7193WO1(01763)

<150>  U.S. Prov. Appl. No.  62/597,717
<151>  2017-12-12

<160>  139   

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NY-ESO epitope

<400>  1

Ser Leu Leu Met Trp Ile Thr Gln Cys 
1               5                   


<210>  2
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8F TCR alpha chain variable region

<400>  2

Glu Glu Asp Pro Gln Ala Leu Ser Ile Gln Glu Gly Glu Asn Ala Thr 
1               5                   10                  15      


Met Asn Cys Ser Tyr Lys Thr Ser Ile Asn Asn Leu Gln Trp Tyr Arg 
            20                  25                  30          


Gln Asn Ser Gly Arg Gly Leu Val His Leu Ile Leu Ile Arg Ser Asn 
        35                  40                  45              


Glu Arg Glu Lys His Ser Gly Arg Leu Arg Val Thr Leu Asp Thr Ser 
    50                  55                  60                  


Lys Lys Ser Ser Ser Leu Leu Ile Thr Ala Ser Arg Ala Ala Asp Thr 
65                  70                  75                  80  


Ala Ser Tyr Phe Cys Ala Thr Asp Gly Ala Gly Lys Ser Thr Phe Gly 
                85                  90                  95      


Asp Gly Thr Thr Leu Thr Val Lys Pro Asn 
            100                 105     


<210>  3
<211>  318
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8F TCR alpha chain

<400>  3
gaggaggacc cccaggccct gtccatccag gagggggaga atgccaccat gaattgcagt       60

tacaagactt ccataaacaa cctgcagtgg taccgccaga actccggccg cggcctggtg      120

cacctgatcc tcatccggtc gaatgaaagg gaaaagcact cgggacgcct gcgagtgact      180

ctggacacgt ccaagaagtc gtccagtctc ttaatcaccg cctctcgcgc agccgatacc      240

gcatcgtact tctgtgcaac cgacggggcg ggcaagagta cattcggcga cggcactacc      300

ctgaccgtga agccaaat                                                    318


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8F TCR CDR1

<400>  4

Thr Ser Ile Asn Asn 
1               5   


<210>  5
<211>  270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8F TCR alpha chain

<400>  5

Met Glu Thr Leu Leu Gly Val Ser Leu Val Ile Leu Trp Leu Gln Leu 
1               5                   10                  15      


Ala Arg Val Asn Ser Gln Gln Gly Glu Glu Asp Pro Gln Ala Leu Ser 
            20                  25                  30          


Ile Gln Glu Gly Glu Asn Ala Thr Met Asn Cys Ser Tyr Lys Thr Ser 
        35                  40                  45              


Ile Asn Asn Leu Gln Trp Tyr Arg Gln Asn Ser Gly Arg Gly Leu Val 
    50                  55                  60                  


His Leu Ile Leu Ile Arg Ser Asn Glu Arg Glu Lys His Ser Gly Arg 
65                  70                  75                  80  


Leu Arg Val Thr Leu Asp Thr Ser Lys Lys Ser Ser Ser Leu Leu Ile 
                85                  90                  95      


Thr Ala Ser Arg Ala Ala Asp Thr Ala Ser Tyr Phe Cys Ala Thr Asp 
            100                 105                 110         


Gly Ala Gly Lys Ser Thr Phe Gly Asp Gly Thr Thr Leu Thr Val Lys 
        115                 120                 125             


Pro Asn Ile Gln Lys Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser 
    130                 135                 140                 


Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln 
145                 150                 155                 160 


Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys 
                165                 170                 175     


Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val 
            180                 185                 190         


Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn 
        195                 200                 205             


Ser Ile Ile Pro Ala Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
    210                 215                 220                 


Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn 
225                 230                 235                 240 


Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val 
                245                 250                 255     


Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
            260                 265                 270 


<210>  6
<211>  924
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8F TCR alpha chain

<400>  6
atggactcgt ggaccttatg ctgcgtgtcc ctgtgcatac tggttgccaa gcacacagac       60

gccggggtga tccagagccc ccggcacgaa gttaccgaga tgggccagga ggtgacgctc      120

cgatgcaagc ccatcagtgg ccacgattat ctcttctggt accgccaaac catgatgcgc      180

ggcttggaac tcctcatcta cttcaacaac aacgtcccca tcgatgactc cggcatgcct      240

gaggacaggt tcagtgcgaa gatgccgaat gcatccttct ccaccctgaa gatacagccg      300

agtgagcccc gcgactccgc tgtgtacttc tgcgcctcta ctatcggcgc ccagcctcaa      360

catttcggcg acggcacgcg cctcagtatc ctggaggacc tgaacaaggt gttccctccg      420

gaagtggctg tgtttgagcc ctccgaggca gaaatctcac acacacagaa ggcaaccctc      480

gtgtgtctgg caacaggttt cttcccagat cacgtggagc tgagttggtg ggtcaacggc      540

aaggaggtcc atagcggggt gagtaccgac ccacagcctc tcaaggagca gcctgccctc      600

aacgacagta ggtactgcct gtcctcgcgc ctccgcgtgt ccgcaacgtt ctggcagaat      660

ccccgcaacc acttccggtg ccaggtccaa ttctacggcc tgagtgagaa cgatgagtgg      720

acacaggata gggccaagcc cgtgacccag atcgtgtccg ccgaggcctg gggccgcgct      780

gactgcggct tcacctccgt gtcgtatcag cagggcgtat tatcagccac cattctttac      840

gaaatcctcc tcggcaaggc cacactatac gccgtgctgg tgtcggcgct ggtgttaatg      900

gcgatggtca agcgaaagga ttaa                                             924


<210>  7
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8F TCR beta chain variable region

<400>  7

Val Ile Gln Ser Pro Arg His Glu Val Thr Glu Met Gly Gln Glu Val 
1               5                   10                  15      


Thr Leu Arg Cys Lys Pro Ile Ser Gly His Asp Tyr Leu Phe Trp Tyr 
            20                  25                  30          


Arg Gln Thr Met Met Arg Gly Leu Glu Leu Leu Ile Tyr Phe Asn Asn 
        35                  40                  45              


Asn Val Pro Ile Asp Asp Ser Gly Met Pro Glu Asp Arg Phe Ser Ala 
    50                  55                  60                  


Lys Met Pro Asn Ala Ser Phe Ser Thr Leu Lys Ile Gln Pro Ser Glu 
65                  70                  75                  80  


Pro Arg Asp Ser Ala Val Tyr Phe Cys Ala Ser Thr Ile Gly Ala Gln 
                85                  90                  95      


Pro Gln His Phe Gly Asp Gly Thr Arg Leu Ser Ile Leu Glu 
            100                 105                 110 


<210>  8
<211>  330
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8F TCR beta chain variable region

<400>  8
gtgatccaga gcccccggca cgaagttacc gagatgggcc aggaggtgac gctccgatgc       60

aagcccatca gtggccacga ttatctcttc tggtaccgcc aaaccatgat gcgcggcttg      120

gaactcctca tctacttcaa caacaacgtc cccatcgatg actccggcat gcctgaggac      180

aggttcagtg cgaagatgcc gaatgcatcc ttctccaccc tgaagataca gccgagtgag      240

ccccgcgact ccgctgtgta cttctgcgcc tctactatcg gcgcccagcc tcaacatttc      300

ggcgacggca cgcgcctcag tatcctggag                                       330


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8F TCR CDR1 VB

<400>  9

Ser Gly His Asp Tyr 
1               5   


<210>  10
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8F TCR CDR2 VB

<400>  10

Phe Asn Asn Asn Val Pro 
1               5       


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8F TCR CDR3 VB

<400>  11

Ala Ser Thr Ile 
1               


<210>  12
<211>  270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  8F TCR beta chain

<400>  12

Met Glu Thr Leu Leu Gly Val Ser Leu Val Ile Leu Trp Leu Gln Leu 
1               5                   10                  15      


Ala Arg Val Asn Ser Gln Gln Gly Glu Glu Asp Pro Gln Ala Leu Ser 
            20                  25                  30          


Ile Gln Glu Gly Glu Asn Ala Thr Met Asn Cys Ser Tyr Lys Thr Ser 
        35                  40                  45              


Ile Asn Asn Leu Gln Trp Tyr Arg Gln Asn Ser Gly Arg Gly Leu Val 
    50                  55                  60                  


His Leu Ile Leu Ile Arg Ser Asn Glu Arg Glu Lys His Ser Gly Arg 
65                  70                  75                  80  


Leu Arg Val Thr Leu Asp Thr Ser Lys Lys Ser Ser Ser Leu Leu Ile 
                85                  90                  95      


Thr Ala Ser Arg Ala Ala Asp Thr Ala Ser Tyr Phe Cys Ala Thr Asp 
            100                 105                 110         


Gly Ala Gly Lys Ser Thr Phe Gly Asp Gly Thr Thr Leu Thr Val Lys 
        115                 120                 125             


Pro Asn Ile Gln Lys Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser 
    130                 135                 140                 


Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln 
145                 150                 155                 160 


Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys 
                165                 170                 175     


Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val 
            180                 185                 190         


Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn 
        195                 200                 205             


Ser Ile Ile Pro Ala Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys 
    210                 215                 220                 


Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn 
225                 230                 235                 240 


Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val 
                245                 250                 255     


Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
            260                 265                 270 


<210>  13
<211>  810
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8F TCR beta chain

<400>  13
atggagaccc tgctcggggt ctcactggtc atcctgtggc tgcagctggc cagggtgaac       60

tcgcagcagg gggaggagga cccccaggcc ctgtccatcc aggaggggga gaatgccacc      120

atgaattgca gttacaagac ttccataaac aacctgcagt ggtaccgcca gaactccggc      180

cgcggcctgg tgcacctgat cctcatccgg tcgaatgaaa gggaaaagca ctcgggacgc      240

ctgcgagtga ctctggacac gtccaagaag tcgtccagtc tcttaatcac cgcctctcgc      300

gcagccgata ccgcatcgta cttctgtgca accgacgggg cgggcaagag tacattcggc      360

gacggcacta ccctgaccgt gaagccaaat atccagaagc ctgatccagc tgtctatcag      420

ttgcgcgatt ccaaatcgtc tgacaaatct gtgtgcctgt tcaccgactt cgactcccag      480

acgaacgtgt cccagagtaa agacagcgac gtgtacatca ctgataagac cgtgctggac      540

atgcgctcca tggactttaa aagtaacagc gctgtagcgt ggagcaacaa gagtgacttc      600

gcctgcgcca acgccttcaa taactctatc atacctgccg ataccttctt cccgagcccc      660

gaatccagtt gcgacgtgaa gctcgtggag aagagctttg agacagacac caacctgaac      720

ttccaaaacc tgtccgtcat tggcttcagg atcctcctcc tcaaggtggc cggcttcaac      780

ttgctcatga cgctgagact ctggagttca                                       810


<210>  14
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-CD28 switch receptor

<400>  14

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Phe Trp Val Leu Val Val 
                165                 170                 175     


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
            180                 185                 190         


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
        195                 200                 205             


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
    210                 215                 220                 


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
225                 230                 235             


<210>  15
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD1-CD28 switch receptor

<400>  15
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtg ttttgggtgc tggtggtggt tggtggagtc      540

ctggcttgct atagcttgct agtaacagtg gcctttatta ttttctgggt gaggagtaag      600

aggagcaggc tcctgcacag tgactacatg aacatgactc cccgccgccc cgggcccacc      660

cgcaagcatt accagcccta tgccccacca cgcgacttcg cagcctatcg ctcc            714


<210>  16
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TGFBRI-IL-12RB1 switch receptor

<400>  16

Leu Glu Ala Ala Val Ala Ala Pro Arg Pro Arg Leu Leu Leu Leu Val 
1               5                   10                  15      


Leu Ala Ala Ala Ala Ala Ala Ala Ala Ala Leu Leu Pro Gly Ala Thr 
            20                  25                  30          


Ala Leu Gln Cys Phe Cys His Leu Cys Thr Lys Asp Asn Phe Thr Cys 
        35                  40                  45              


Val Thr Asp Gly Leu Cys Phe Val Ser Val Thr Glu Thr Thr Asp Lys 
    50                  55                  60                  


Val Ile His Asn Ser Met Cys Ile Ala Glu Ile Asp Leu Ile Pro Arg 
65                  70                  75                  80  


Asp Arg Pro Phe Val Cys Ala Pro Ser Ser Lys Thr Gly Ser Val Thr 
                85                  90                  95      


Thr Thr Tyr Cys Cys Asn Gln Asp His Cys Asn Lys Ile Glu Leu Pro 
            100                 105                 110         


Thr Thr Val Lys Ser Ser Pro Gly Leu Gly Pro Val Glu Leu Ala Ala 
        115                 120                 125             


Val Ile Ala Gly Pro Val Cys Phe Val Cys Ile Ser Leu Met Leu Met 
    130                 135                 140                 


Val Tyr Ile Arg Ala Ala Arg His Leu Cys Pro Pro Leu Pro Thr Pro 
145                 150                 155                 160 


Cys Ala Ser Ser Ala Ile Glu Phe Pro Gly Gly Lys Glu Thr Trp Gln 
                165                 170                 175     


Trp Ile Asn Pro Val Asp Phe Gln Glu Glu Ala Ser Leu Gln Glu Ala 
            180                 185                 190         


Leu Val Val Glu Met Ser Trp Asp Lys Gly Glu Arg Thr Glu Pro Leu 
        195                 200                 205             


Glu Lys Thr Glu Leu Pro Glu Gly Ala Pro Glu Leu Ala Leu Asp Thr 
    210                 215                 220                 


Glu Leu Ser Leu Glu Asp Gly Asp Arg Cys Lys Ala Lys Met 
225                 230                 235             


<210>  17
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TGFBRI-IL-12RB1 switch receptor

<400>  17
ctggaggcgg cggtcgctgc tccgcgtccc cggctgctcc tcctcgtgct ggcggcggcg       60

gcggcggcgg cggcggcgct gctcccgggg gcgacggcgt tacagtgttt ctgccacctc      120

tgtacaaaag acaattttac ttgtgtgaca gatgggctct gctttgtctc tgtcacagag      180

accacagaca aagttataca caacagcatg tgtatagctg aaattgactt aattcctcga      240

gataggccgt ttgtatgtgc accctcttca aaaactgggt ctgtgactac aacatattgc      300

tgcaatcagg accattgcaa taaaatagaa cttccaacta ctgtaaagtc atcacctggc      360

cttggtcctg tggaactggc agctgtcatt gctggaccag tgtgcttcgt ctgcatctca      420

ctcatgttga tggtctatat cagggccgca cggcacctgt gcccgccgct gcccacaccc      480

tgtgccagct ccgccattga gttccctgga gggaaggaga cttggcagtg gatcaaccca      540

gtggacttcc aggaagaggc atccctgcag gaggccctgg tggtagagat gtcctgggac      600

aaaggcgaga ggactgagcc tctcgagaag acagagctac ctgagggtgc ccctgagctg      660

gccctggata cagagttgtc cttggaggat ggagacaggt gcaaggccaa gatgtga         717


<210>  18
<211>  403
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TGFBRII-IL-12RB2 switch receptor

<400>  18

Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu His Ile Val Leu 
1               5                   10                  15      


Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val Gln Lys Ser Val 
            20                  25                  30          


Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro 
        35                  40                  45              


Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln 
    50                  55                  60                  


Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro 
65                  70                  75                  80  


Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr 
                85                  90                  95      


Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile 
            100                 105                 110         


Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys 
        115                 120                 125             


Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn 
    130                 135                 140                 


Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu 
145                 150                 155                 160 


Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro Pro Leu 
                165                 170                 175     


Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Gln Gln Lys Val Phe 
            180                 185                 190         


Val Leu Leu Ala Ala Leu Arg Pro Gln Trp Cys Ser Arg Glu Ile Pro 
        195                 200                 205             


Asp Pro Ala Asn Ser Thr Cys Ala Lys Lys Tyr Pro Ile Ala Glu Glu 
    210                 215                 220                 


Lys Thr Gln Leu Pro Leu Asp Arg Leu Leu Ile Asp Trp Pro Thr Pro 
225                 230                 235                 240 


Glu Asp Pro Glu Pro Leu Val Ile Ser Glu Val Leu His Gln Val Thr 
                245                 250                 255     


Pro Val Phe Arg His Pro Pro Cys Ser Asn Trp Pro Gln Arg Glu Lys 
            260                 265                 270         


Gly Ile Gln Gly His Gln Ala Ser Glu Lys Asp Met Met His Ser Ala 
        275                 280                 285             


Ser Ser Pro Pro Pro Pro Arg Ala Leu Gln Ala Glu Ser Arg Gln Leu 
    290                 295                 300                 


Val Asp Leu Tyr Lys Val Leu Glu Ser Arg Gly Ser Asp Pro Lys Pro 
305                 310                 315                 320 


Glu Asn Pro Ala Cys Pro Trp Thr Val Leu Pro Ala Gly Asp Leu Pro 
                325                 330                 335     


Thr His Asp Gly Tyr Leu Pro Ser Asn Ile Asp Asp Leu Pro Ser His 
            340                 345                 350         


Glu Ala Pro Leu Ala Asp Ser Leu Glu Glu Leu Glu Pro Gln His Ile 
        355                 360                 365             


Ser Leu Ser Val Phe Pro Ser Ser Ser Leu His Pro Leu Thr Phe Ser 
    370                 375                 380                 


Cys Gly Asp Lys Leu Thr Leu Asp Gln Leu Lys Met Arg Cys Asp Ser 
385                 390                 395                 400 


Leu Met Leu 
            


<210>  19
<211>  1212
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TGFBRII-IL-12RB2 switch receptor

<400>  19
atgggtcggg ggctgctcag gggcctgtgg ccgctgcaca tcgtcctgtg gacgcgtatc       60

gccagcacga tcccaccgca cgttcagaag tcggttaata acgacatgat agtcactgac      120

aacaacggtg cagtcaagtt tccacaactg tgtaaatttt gtgatgtgag attttccacc      180

tgtgacaacc agaaatcctg catgagcaac tgcagcatca cctccatctg tgagaagcca      240

caggaagtct gtgtggctgt atggagaaag aatgacgaga acataacact agagacagtt      300

tgccatgacc ccaagctccc ctaccatgac tttattctgg aagatgctgc ttctccaaag      360

tgcattatga aggaaaaaaa aaagcctggt gagactttct tcatgtgttc ctgtagctct      420

gatgagtgca atgacaacat catcttctca gaagaatata acaccagcaa tcctgacttg      480

ttgctagtca tatttcaagt gacaggcatc agcctcctgc caccactggg agttgccata      540

tctgtcatca tcatcttcta ccagcaaaag gtgtttgttc tcctagcagc cctcagacct      600

cagtggtgta gcagagaaat tccagatcca gcaaatagca cttgcgctaa gaaatatccc      660

attgcagagg agaagacaca gctgcccttg gacaggctcc tgatagactg gcccacgcct      720

gaagatcctg aaccgctggt catcagtgaa gtccttcatc aagtgacccc agttttcaga      780

catcccccct gctccaactg gccacaaagg gaaaaaggaa tccaaggtca tcaggcctct      840

gagaaagaca tgatgcacag tgcctcaagc ccaccacctc caagagctct ccaagctgag      900

agcagacaac tggtggatct gtacaaggtg ctggagagca ggggctccga cccaaagcca      960

gaaaacccag cctgtccctg gacggtgctc ccagcaggtg accttcccac ccatgatggc     1020

tacttaccct ccaacataga tgacctcccc tcacatgagg cacctctcgc tgactctctg     1080

gaagaactgg agcctcagca catctccctt tctgttttcc cctcaagttc tcttcaccca     1140

ctcaccttct cctgtggtga taagctgact ctggatcagt taaagatgag gtgtgactcc     1200

ctcatgctct ga                                                         1212


<210>  20
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TGFBRIIDN switch receptor

<400>  20

Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu His Ile Val Leu 
1               5                   10                  15      


Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val Gln Lys Ser Val 
            20                  25                  30          


Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro 
        35                  40                  45              


Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln 
    50                  55                  60                  


Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro 
65                  70                  75                  80  


Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr 
                85                  90                  95      


Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile 
            100                 105                 110         


Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys 
        115                 120                 125             


Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn 
    130                 135                 140                 


Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu 
145                 150                 155                 160 


Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro Pro Leu 
                165                 170                 175     


Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Cys Tyr Arg Val Asn 
            180                 185                 190         


Arg Gln Gln Lys Leu Ser Ser Ser Gly 
        195                 200     


<210>  21
<211>  603
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TGFBRIIDN switch receptor

<400>  21
atgggtcggg ggctgctcag gggcctgtgg ccgctgcaca tcgtcctgtg gacgcgtatc       60

gccagcacga tcccaccgca cgttcagaag tcggttaata acgacatgat agtcactgac      120

aacaacggtg cagtcaagtt tccacaactg tgtaaatttt gtgatgtgag attttccacc      180

tgtgacaacc agaaatcctg catgagcaac tgcagcatca cctccatctg tgagaagcca      240

caggaagtct gtgtggctgt atggagaaag aatgacgaga acataacact agagacagtt      300

tgccatgacc ccaagctccc ctaccatgac tttattctgg aagatgctgc ttctccaaag      360

tgcattatga aggaaaaaaa aaagcctggt gagactttct tcatgtgttc ctgtagctct      420

gatgagtgca atgacaacat catcttctca gaagaatata acaccagcaa tcctgacttg      480

ttgctagtca tatttcaagt gacaggcatc agcctcctgc caccactggg agttgccata      540

tctgtcatca tcatcttcta ctgctaccgc gttaaccggc agcagaagct gagttcatcc      600

gga                                                                    603


<210>  22
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage sites


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  22

Arg Xaa Lys Arg 
1               


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage sites


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  23

Arg Xaa Arg Arg 
1               


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage sites


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be K or R

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occuring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be K or R

<400>  24

Xaa Arg Xaa Xaa Arg 
1               5   


<210>  25
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage sites


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  25

Arg Xaa Xaa Arg 
1               


<210>  26
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  furin cleavage sites

<400>  26

Arg Gln Lys Arg 
1               


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence


<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeat n times, where n represents an integer of at least 1

<400>  27

Gly Ser Gly Gly Ser 
1               5   


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence


<220>
<221>  REPEAT
<222>  (1)..(4)
<223>  repeat n times, where n represents an integer of at least 1

<400>  28

Gly Gly Gly Ser 
1               


<210>  29
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  29

Gly Gly Ser Gly 
1               


<210>  30
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  30

Gly Gly Ser Gly Gly 
1               5   


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  31

Gly Ser Gly Ser Gly 
1               5   


<210>  32
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  32

Gly Ser Gly Gly Gly 
1               5   


<210>  33
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  33

Gly Gly Gly Ser Gly 
1               5   


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  34

Gly Ser Ser Ser Gly 
1               5   


<210>  35
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F-GS2-T2A linker

<400>  35
cgtgcgaaga ggggcggcgg gggctccggc gggggaggca gtgagggccg cggctccctg       60

ctgacctgcg gagatgtaga agagaaccca ggcccc                                 96


<210>  36
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F-GS2-T2A linker

<400>  36

Arg Ala Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Gly 
1               5                   10                  15      


Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
            20                  25                  30          


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  37
gagaatcaaa atcggtgaat                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  38
tgtgctagac atgaggtcta                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  39
aaagtcagat ttgttgctcc                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  40
agagtctctc agctggtaca                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  41
agctggtaca cggcagggtc                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  42
acaaaactgt gctagacatg                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  43
ctcgaccagc ttgacatcac                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  44
aagttcctgt gatgtcaagc                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  45
ttcggaaccc aatcactgac                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  46
ttaatctgct catgacgctg                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  47
gattaaaccc ggccactttc                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  48
cgtcatgagc agattaaacc                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  49
taaacccggc cactttcagg                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  50
caaacacagc gacctcgggt                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  51
ggctcaaaca cagcgacctc                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  52
tcaaacacag cgacctcggg                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  53
tggctcaaac acagcgacct                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  54
tctccgagag cccgtagaac                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  55
ggctctcgga gaatgacgag                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  56
tgacagcgga agtggttgcg                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  57
agtccagttc tacgggctct                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  58
cgctgtcaag tccagttcta                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  59
agctcagctc cacgtggtcg                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  60
actggacttg acagcggaag                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  61
ttgacagcgg aagtggttgc                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  62
gacagcggaa gtggttgcgg                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  63
tgacgagtgg acccaggata                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  64
cgtagaactg gacttgacag                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  65
atgacgagtg gacccaggat                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  66
cttgacagcg gaagtggttg                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  67
gctgtcaagt ccagttctac                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  68
aggcctcggc gctgacgatc                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  69
ggcctcggcg ctgacgatct                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  70
cacccagatc gtcagcgccg                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  71
gacgatctgg gtgacgggtt                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  72
gatcgtcagc gccgaggcct                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  73
agatcgtcag cgccgaggcc                                                   20


<210>  74
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  74
tgtagcaccg cccagacgac                                                   20


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  75
cgtctgggcg gtgctacaac                                                   20


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  76
gtctgggcgg tgctacaact                                                   20


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  77
aggcgccctg gccagtcgtc                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  78
caccgcccag acgactggcc                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  79
atgtggaagt cacgcccgtt                                                   20


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  80
catgtggaag tcacgcccgt                                                   20


<210>  81
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  81
cacgaagctc tccgatgtgt                                                   20


<210>  82
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  82
cggagagctt cgtgctaaac                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  83
cctgctcgtg gtgaccgaag                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  84
ccccttcggt caccacgagc                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  85
aggcggccag cttgtccgtc                                                   20


<210>  86
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  86
gccctgctcg tggtgaccga                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  87
cccttcggtc accacgagca                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  88
ccctgctcgt ggtgaccgaa                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  89
gcgtgacttc cacatgagcg                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  90
aggtgccgct gtcattgcgc                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  91
acttccacat gagcgtggtc                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  92
ggtgccgctg tcattgcgcc                                                   20


<210>  93
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  93
accctggtgg ttggtgtcgt                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  94
agggtttgga actggccggc                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  95
attgtctttc ctagcggaat                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  96
tcagtggctg ggcactccga                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  97
cattgtcttt cctagcggaa                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  98
aatgtgactc tagcagacag                                                   20


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  99
atgagaatac cctagtaagg                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  100
tatgagaata ccctagtaag                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  101
tggcccaggt aactatgcat                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  102
ataggcatct acatcggagc                                                   20


<210>  103
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  103
gctgtggaaa taaagtgttg                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  104
gtggaataca gagcggaggt                                                   20


<210>  105
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  105
acagtgggat ctactgctgc                                                   20


<210>  106
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  106
tctctctgcc gagtcggtgc                                                   20


<210>  107
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  107
ttatgcctgg gatttggatc                                                   20


<210>  108
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  108
atcagaatag gcatctacat                                                   20


<210>  109
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  109
tgagttacgg gactctagat                                                   20


<210>  110
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  110
gccaatgtgg atatttgcta                                                   20


<210>  111
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  111
gtgaagtctc tctgccgagt                                                   20


<210>  112
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  112
tcagggacac atctcctttg                                                   20


<210>  113
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  113
gggcacgagg ttccctgggg                                                   20


<210>  114
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  114
aaataaggtg gttggatcta                                                   20


<210>  115
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  115
ctaaatgggg atttccgcaa                                                   20


<210>  116
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  116
aatgtggcaa cgtggtgctc                                                   20


<210>  117
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  117
atccccattt agccagtatc                                                   20


<210>  118
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  118
tgctgccgga tccaaatccc                                                   20


<210>  119
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  119
gaacctcgtg cccgtctgct                                                   20


<210>  120
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  120
cagacgggca cgaggttccc                                                   20


<210>  121
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  121
agacgggcac gaggttccct                                                   20


<210>  122
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  122
ctctctgccg agtcggtgca                                                   20


<210>  123
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  123
tctctgccga gtcggtgcag                                                   20


<210>  124
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  124
aggtcacccc tgcaccgact                                                   20


<210>  125
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  125
taggcatcta catcggagca                                                   20


<210>  126
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  126
tagattggcc aatgacttac                                                   20


<210>  127
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  127
ggcgccctgg ccagtcgtct                                                   20


<210>  128
<211>  101
<212>  DNA
<213>  Homo sapiens

<400>  128
aacaaatgtg tcacaaagta aggattctga tgtgtatatc acagacaaaa ctgtgctaga       60

catgaggtct atggacttca agagcaacag tgctgtggcc t                          101


<210>  129
<211>  101
<212>  DNA
<213>  Homo sapiens

<400>  129
gcagaacccc cgcaaccact tccgctgtca agtccagttc tacgggctct cggagaatga       60

cgagtggacc caggataggg ccaaacctgt cacccagatc g                          101


<210>  130
<211>  101
<212>  DNA
<213>  Homo sapiens

<400>  130
gctgctccag gcatgcagat cccacaggcg ccctggccag tcgtctgggc ggtgctacaa       60

ctgggctggc ggccaggatg gttcttaggt aggtggggtc g                          101


<210>  131
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  131
ggagaatgac gagtggaccc                                                   20


<210>  132
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TIM3-CD28 switch receptor

<400>  132

Met Phe Ser His Leu Pro Phe Asp Cys Val Leu Leu Leu Leu Leu Leu 
1               5                   10                  15      


Leu Leu Thr Arg Ser Ser Glu Val Glu Tyr Arg Ala Glu Val Gly Gln 
            20                  25                  30          


Asn Ala Tyr Leu Pro Cys Phe Tyr Thr Pro Ala Ala Pro Gly Asn Leu 
        35                  40                  45              


Val Pro Val Cys Trp Gly Lys Gly Ala Cys Pro Val Phe Glu Cys Gly 
    50                  55                  60                  


Asn Val Val Leu Arg Thr Asp Glu Arg Asp Val Asn Tyr Trp Thr Ser 
65                  70                  75                  80  


Arg Tyr Trp Leu Asn Gly Asp Phe Arg Lys Gly Asp Val Ser Leu Thr 
                85                  90                  95      


Ile Glu Asn Val Thr Leu Ala Asp Ser Gly Ile Tyr Cys Cys Arg Ile 
            100                 105                 110         


Gln Ile Pro Gly Ile Met Asn Asp Glu Lys Phe Asn Leu Lys Leu Val 
        115                 120                 125             


Ile Lys Pro Ala Lys Val Thr Pro Ala Pro Thr Arg Gln Arg Asp Phe 
    130                 135                 140                 


Thr Ala Ala Phe Pro Arg Met Leu Thr Thr Arg Gly His Gly Pro Ala 
145                 150                 155                 160 


Glu Thr Gln Thr Leu Gly Ser Leu Pro Asp Ile Asn Leu Thr Gln Ile 
                165                 170                 175     


Ser Thr Leu Ala Asn Glu Leu Arg Asp Ser Arg Leu Ala Asn Asp Leu 
            180                 185                 190         


Arg Asp Ser Gly Ala Thr Ile Arg Phe Trp Val Leu Val Val Val Gly 
        195                 200                 205             


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
    210                 215                 220                 


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
225                 230                 235                 240 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
                245                 250                 255     


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
            260                 265             


<210>  133
<211>  804
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TIM3-CD28 switch receptor

<400>  133
atgttttcac atcttccctt tgactgtgtc ctgctgctgc tgctgctact acttacaagg       60

tcctcagaag tggaatacag agcggaggtc ggtcagaatg cctatctgcc ctgcttctac      120

accccagccg ccccagggaa cctcgtgccc gtctgctggg gcaaaggagc ctgtcctgtg      180

tttgaatgtg gcaacgtggt gctcaggact gatgaaaggg atgtgaatta ttggacatcc      240

agatactggc taaatgggga tttccgcaaa ggagatgtgt ccctgaccat agagaatgtg      300

actctagcag acagtgggat ctactgctgc cgaatccaaa tcccaggcat aatgaatgat      360

gaaaaattta acctgaagtt ggtcatcaaa ccagccaagg tcacccctgc accgactcgg      420

cagagagact tcactgcagc ctttccaagg atgcttacca ccaggggaca tggcccagca      480

gagacacaga cactggggag cctccctgac ataaatctaa cacaaatatc cacattggcc      540

aatgagttac gggactctag gttggccaat gacttacggg actccggagc aaccatcaga      600

ttttgggtgc tggtggtggt tggtggagtc ctggcttgct atagcttact agtaacagtg      660

gcctttatta ttttctgggt gaggagtaag aggagcaggc tcctgcacag tgactacatg      720

aacatgactc cccgccgccc cgggcccacc cgcaagcatt accagcccta tgccccacca      780

cgcgacttcg cagcctatcg ctcc                                             804


<210>  134
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1-41BB switch receptor

<400>  134

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Ile Tyr Ile Trp Ala Pro 
                165                 170                 175     


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
            180                 185                 190         


Tyr Cys Lys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
        195                 200                 205             


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
    210                 215                 220                 


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
225                 230                 235         


<210>  135
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD1-41BB switch receptor

<400>  135
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtt atctacatct gggcgccctt ggccgggact      540

tgtggggtcc ttctcctgtc actggttatc accctttact gcaaaaaacg gggcagaaag      600

aaactcctgt atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa      660

gatggctgta gctgccgatt tccagaagaa gaagaaggag gatgtgaact g               711


<210>  136
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1A132L-41BB switch receptor

<400>  136

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Leu Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Ile Tyr Ile Trp Ala Pro 
                165                 170                 175     


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
            180                 185                 190         


Tyr Cys Lys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
        195                 200                 205             


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
    210                 215                 220                 


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
225                 230                 235         


<210>  137
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD1A132L-41BB switch receptor

<400>  137
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aagctgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtt atctacatct gggcgccctt ggccgggact      540

tgtggggtcc ttctcctgtc actggttatc accctttact gcaaaaaacg gggcagaaag      600

aaactcctgt atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa      660

gatggctgta gctgccgatt tccagaagaa gaagaaggag gatgtgaact g               711


<210>  138
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PD1A132L-CD28 switch receptor

<400>  138

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Leu Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Phe Trp Val Leu Val Val 
                165                 170                 175     


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
            180                 185                 190         


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
        195                 200                 205             


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
    210                 215                 220                 


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
225                 230                 235             


<210>  139
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PD1A132L-CD28 switch receptor

<400>  139
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aagctgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtg ttttgggtgc tggtggtggt tggtggagtc      540

ctggcttgct atagcttgct agtaacagtg gcctttatta ttttctgggt gaggagtaag      600

aggagcaggc tcctgcacag tgactacatg aacatgactc cccgccgccc cgggcccacc      660

cgcaagcatt accagcccta tgccccacca cgcgacttcg cagcctatcg ctcc            714


