                         SEQUENCE LISTING

<110>  the University of Manitoba
       Yao, Xiaojian
       Ao, Zhujun
       Kobinger, Gary
 
<120>  Ebola Virus and Marburg Virus Glycoprotein Mucin-Like Domain 
       Replacement Expression System used as New Vaccine Approaches

<130>  82402-273PCT

<150>  US 62/598131
<151>  2017-12-13

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  1500
<212>  DNA
<213>  Ebola virus


<220>
<221>  misc_feature
<222>  (918)..(919)
<223>  Deletion of mucin like domain

<400>  1
atgggtgtga ccggtatcct gcagctgccg cgtgatcgct tcaaacgtac ctctttcttt       60

ctgtgggtta tcatcctgtt ccagcgtacc ttttctatcc cgctgggtgt tattcataac      120

tccaccctgc aggtgagcga cgttgataaa ctggtttgcc gtgacaaact gtcttctacc      180

aaccagctgc gctccgtggg cctgaacctg gaaggtaacg gtgttgcaac cgacgtgccg      240

tctgcgacca aacgctgggg tttccgctcc ggtgttccgc cgaaagttgt taactacgaa      300

gcgggcgaat gggctgaaaa ctgttataac ctggaaatca agaaaccgga cggctccgag      360

tgcctgccgg cagctccgga cggtattcgc ggctttccgc gctgtcgtta cgttcataaa      420

gttagcggta ctggtccgtg cgcaggtgac tttgctttcc acaaagaggg cgcgtttttc      480

ctgtatgacc gcctggcatc caccgttatt taccgtggca ccaccttcgc ggaaggcgtt      540

gtggcgttcc tgatcctgcc gcaggctaag aaagatttct ttagcagcca cccgctgcgc      600

gagccggtta acgcgactga ggatccgtct tctggttatt actccaccac tatccgttac      660

caggcaactg gtttcggtac caacgaaact gaatacctgt tcgaagttga taacctgacc      720

tacgttcagc tggaaagccg cttcactccg cagttcctgc tgcagctgaa cgaaaccatc      780

tacaccagcg gtaaacgttc caacaccacc ggcaaactga tctggaaagt taacccggag      840

atcgatacca ctattggtga gtgggcgttt tgggaaacca agaaaaacct gacccgcaaa      900

atccgttccg aggaaggaaa caccatcgca ggtgttgctg gtctgatcac cggcggtcgt      960

cgtacccgcc gtgaagctat tgttaacgca cagccgaaat gtaacccgaa cctgcactac     1020

tggaccactc aggatgaagg cgctgctatc ggcctggcat ggatcccgta cttcggtccg     1080

gcggctgaag gtatctatat cgaaggtctg atgcacaacc aggatggtct gatttgcggt     1140

ctgcgtcagc tggcgaacga aaccactcag gcgctgcagc tgttcctgcg cgcaaccacc     1200

gagctgcgta ccttctctat cctgaaccgt aaggcgatcg actttctgct gcagcgttgg     1260

ggtggtacct gccatatcct gggtccggac tgctgtatcg agccgcatga ttggactaaa     1320

aacatcactg acaaaatcga ccagatcatt cacgacttcg ttgacaaaac cctgccggac     1380

cagggcgata acgacaactg gtggaccggc tggcgtcagt ggattccggc aggcatcggc     1440

gttaccggtg ttattattgc tgtgattgca ctgttttgca tttgcaagtt cgttttctga     1500


<210>  2
<211>  499
<212>  PRT
<213>  ebola virus

<400>  2

Met Gly Val Thr Gly Ile Leu Gln Leu Pro Arg Asp Arg Phe Lys Arg 
1               5                   10                  15      


Thr Ser Phe Phe Leu Trp Val Ile Ile Leu Phe Gln Arg Thr Phe Ser 
            20                  25                  30          


Ile Pro Leu Gly Val Ile His Asn Ser Thr Leu Gln Val Ser Asp Val 
        35                  40                  45              


Asp Lys Leu Val Cys Arg Asp Lys Leu Ser Ser Thr Asn Gln Leu Arg 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Val Ala Thr Asp Val Pro 
65                  70                  75                  80  


Ser Ala Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Asn Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Glu 
            100                 105                 110         


Ile Lys Lys Pro Asp Gly Ser Glu Cys Leu Pro Ala Ala Pro Asp Gly 
        115                 120                 125             


Ile Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Val Ser Gly Thr 
    130                 135                 140                 


Gly Pro Cys Ala Gly Asp Phe Ala Phe His Lys Glu Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Thr Thr Phe 
                165                 170                 175     


Ala Glu Gly Val Val Ala Phe Leu Ile Leu Pro Gln Ala Lys Lys Asp 
            180                 185                 190         


Phe Phe Ser Ser His Pro Leu Arg Glu Pro Val Asn Ala Thr Glu Asp 
        195                 200                 205             


Pro Ser Ser Gly Tyr Tyr Ser Thr Thr Ile Arg Tyr Gln Ala Thr Gly 
    210                 215                 220                 


Phe Gly Thr Asn Glu Thr Glu Tyr Leu Phe Glu Val Asp Asn Leu Thr 
225                 230                 235                 240 


Tyr Val Gln Leu Glu Ser Arg Phe Thr Pro Gln Phe Leu Leu Gln Leu 
                245                 250                 255     


Asn Glu Thr Ile Tyr Thr Ser Gly Lys Arg Ser Asn Thr Thr Gly Lys 
            260                 265                 270         


Leu Ile Trp Lys Val Asn Pro Glu Ile Asp Thr Thr Ile Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Thr Lys Lys Asn Leu Thr Arg Lys Ile Arg Ser Glu 
    290                 295                 300                 


Glu Gly Asn Thr Ile Ala Gly Val Ala Gly Leu Ile Thr Gly Gly Arg 
305                 310                 315                 320 


Arg Thr Arg Arg Glu Ala Ile Val Asn Ala Gln Pro Lys Cys Asn Pro 
                325                 330                 335     


Asn Leu His Tyr Trp Thr Thr Gln Asp Glu Gly Ala Ala Ile Gly Leu 
            340                 345                 350         


Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu Gly Ile Tyr Ile Glu 
        355                 360                 365             


Gly Leu Met His Asn Gln Asp Gly Leu Ile Cys Gly Leu Arg Gln Leu 
    370                 375                 380                 


Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr Thr 
385                 390                 395                 400 


Glu Leu Arg Thr Phe Ser Ile Leu Asn Arg Lys Ala Ile Asp Phe Leu 
                405                 410                 415     


Leu Gln Arg Trp Gly Gly Thr Cys His Ile Leu Gly Pro Asp Cys Cys 
            420                 425                 430         


Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asp Gln 
        435                 440                 445             


Ile Ile His Asp Phe Val Asp Lys Thr Leu Pro Asp Gln Gly Asp Asn 
    450                 455                 460                 


Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp Ile Pro Ala Gly Ile Gly 
465                 470                 475                 480 


Val Thr Gly Val Ile Ile Ala Val Ile Ala Leu Phe Cys Ile Cys Lys 
                485                 490                 495     


Phe Val Phe 
            


<210>  3
<211>  402
<212>  DNA
<213>  Human immunodeficiency virus

<400>  3
ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca tggaatcagg       60

ccagtagtat caactcaact gctgttaaat ggcagtctag cagaagaaga tgtagtaatt      120

agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa cacatctgta      180

gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat ccagagggga      240

ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc acattgtaac      300

attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt aagagaacaa      360

tttggaaata ataaaacaat aatctttaag caatcctcag ga                         402


<210>  4
<211>  134
<212>  PRT
<213>  Human immunodeficiency virus

<400>  4

Phe Asn Gly Thr Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr 
1               5                   10                  15      


His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser 
            20                  25                  30          


Leu Ala Glu Glu Asp Val Val Ile Arg Ser Ala Asn Phe Thr Asp Asn 
        35                  40                  45              


Ala Lys Thr Ile Ile Val Gln Leu Asn Thr Ser Val Glu Ile Asn Cys 
    50                  55                  60                  


Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gln Arg Gly 
65                  70                  75                  80  


Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met Arg Gln 
                85                  90                  95      


Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu Lys Gln 
            100                 105                 110         


Ile Ala Ser Lys Leu Arg Glu Gln Phe Gly Asn Asn Lys Thr Ile Ile 
        115                 120                 125             


Phe Lys Gln Ser Ser Gly 
    130                 


<210>  5
<211>  723
<212>  DNA
<213>  Human immunodeficiency virus

<400>  5
ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca tggaatcagg       60

ccagtagtat caactcaact gctgttaaat ggcagtctag cagaagaaga tgtagtaatt      120

agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa cacatctgta      180

gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat ccagagggga      240

ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc acattgtaac      300

attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt aagagaacaa      360

tttggaaata ataaaacaat aatctttaag caatcctcag gaggggaccc agaaattgta      420

acgcacagtt ttaattgtgg aggggaattt ttctactgta attcaacaca actgtttaat      480

agtacttggt ttaatagtac ttggagtact gaagggtcaa ataacactga aggaagtgac      540

acaatcacac tcccatgcag aataaaacaa tttataaaca tgtggcagga agtaggaaaa      600

gcaatgtatg cccctcccat cagtggacaa attagatgtt catcaaatat tactgggctg      660

ctattaacaa gagatggtgg taataacaac aatgggtccg agatcttcag acctggagga      720

ggc                                                                    723


<210>  6
<211>  241
<212>  PRT
<213>  Human immunodeficiency virus

<400>  6

Phe Asn Gly Thr Gly Pro Cys Thr Asn Val Ser Thr Val Gln Cys Thr 
1               5                   10                  15      


His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser 
            20                  25                  30          


Leu Ala Glu Glu Asp Val Val Ile Arg Ser Ala Asn Phe Thr Asp Asn 
        35                  40                  45              


Ala Lys Thr Ile Ile Val Gln Leu Asn Thr Ser Val Glu Ile Asn Cys 
    50                  55                  60                  


Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gln Arg Gly 
65                  70                  75                  80  


Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met Arg Gln 
                85                  90                  95      


Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu Lys Gln 
            100                 105                 110         


Ile Ala Ser Lys Leu Arg Glu Gln Phe Gly Asn Asn Lys Thr Ile Ile 
        115                 120                 125             


Phe Lys Gln Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His Ser Phe 
    130                 135                 140                 


Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gln Leu Phe Asn 
145                 150                 155                 160 


Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn Asn Thr 
                165                 170                 175     


Glu Gly Ser Asp Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Phe Ile 
            180                 185                 190         


Asn Met Trp Gln Glu Val Gly Lys Ala Met Tyr Ala Pro Pro Ile Ser 
        195                 200                 205             


Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg 
    210                 215                 220                 


Asp Gly Gly Asn Asn Asn Asn Gly Ser Glu Ile Phe Arg Pro Gly Gly 
225                 230                 235                 240 


Gly 
    


<210>  7
<211>  306
<212>  PRT
<213>  Zika Virus

<400>  7

Gly Thr Gly Thr Cys Ala Thr Ala Cys Thr Cys Ala Cys Thr Gly Thr 
1               5                   10                  15      


Gly Cys Ala Cys Thr Gly Cys Ala Gly Cys Ala Thr Thr Cys Ala Cys 
            20                  25                  30          


Ala Thr Thr Cys Ala Cys Cys Ala Ala Gly Gly Thr Gly Cys Cys Ala 
        35                  40                  45              


Gly Cys Thr Gly Ala Gly Ala Cys Ala Cys Thr Gly Cys Ala Cys Gly 
    50                  55                  60                  


Gly Thr Ala Cys Thr Gly Thr Gly Ala Cys Ala Gly Thr Gly Gly Ala 
65                  70                  75                  80  


Ala Gly Thr Gly Cys Ala Gly Thr Ala Cys Gly Cys Thr Gly Gly Thr 
                85                  90                  95      


Ala Cys Cys Gly Ala Cys Gly Gly Thr Cys Cys Thr Thr Gly Cys Ala 
            100                 105                 110         


Ala Gly Ala Thr Cys Cys Cys Ala Gly Thr Gly Cys Ala Gly Ala Thr 
        115                 120                 125             


Gly Gly Cys Ala Gly Thr Ala Gly Ala Cys Ala Thr Gly Cys Ala Gly 
    130                 135                 140                 


Ala Cys Thr Cys Thr Gly Ala Cys Thr Cys Cys Ala Gly Thr Gly Gly 
145                 150                 155                 160 


Gly Ala Cys Gly Ala Cys Thr Gly Ala Thr Cys Ala Cys Thr Gly Cys 
                165                 170                 175     


Thr Ala Ala Cys Cys Cys Thr Gly Thr Gly Ala Thr Cys Ala Cys Thr 
            180                 185                 190         


Gly Ala Gly Thr Cys Ala Ala Cys Thr Gly Ala Gly Ala Ala Cys Thr 
        195                 200                 205             


Cys Cys Ala Ala Gly Ala Thr Gly Ala Thr Gly Cys Thr Gly Gly Ala 
    210                 215                 220                 


Ala Cys Thr Gly Gly Ala Cys Cys Cys Ala Cys Cys Ala Thr Thr Cys 
225                 230                 235                 240 


Gly Gly Ala Gly Ala Cys Thr Cys Cys Thr Ala Cys Ala Thr Cys Gly 
                245                 250                 255     


Thr Gly Ala Thr Cys Gly Gly Cys Gly Thr Gly Gly Gly Thr Gly Ala 
            260                 265                 270         


Cys Ala Ala Gly Ala Ala Gly Ala Thr Cys Ala Cys Cys Cys Ala Thr 
        275                 280                 285             


Cys Ala Cys Thr Gly Gly Cys Ala Thr Cys Gly Ala Thr Cys Cys Gly 
    290                 295                 300                 


Gly Cys 
305     


<210>  8
<211>  102
<212>  PRT
<213>  Zika virus

<400>  8

Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro 
1               5                   10                  15      


Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            20                  25                  30          


Thr Asp Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln 
        35                  40                  45              


Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    50                  55                  60                  


Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
65                  70                  75                  80  


Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His 
                85                  90                  95      


His Trp His Arg Ser Gly 
            100         


<210>  9
<211>  1542
<212>  DNA
<213>  Marburg virus


<220>
<221>  misc_feature
<222>  (772)..(773)
<223>  deletion of mucin-like domain

<400>  9
atgaagacta catgtctctt tatcggtctt atcttgatcc aaaggataaa aactcttcct       60

attttagaga tagctagtaa caaccaaccc caaaatatgg attcggtatg ctccggaact      120

ctccagaaga cagaagatgt ccatctgatg ggattcacac tgagtgggca aaaagttgct      180

gattcccctt tggaggcatc caagcgatgg gctttcagga caggtgtacc tcccaagaat      240

gttgagtata cagaagggga ggaagccaaa acatgctaca atataagtgt aacggatccc      300

tctggaaaat ccttgctgtt ggatcctcct accaacatcc gtgactatcc taaatgcaaa      360

actatccatc atattcaagg tcaaaaccct catgcgcaag ggatcgccct ccatctgtgg      420

ggagcatttt tcctgtatga tcgcattgcc tccacaacaa tgtaccgagg cagagtcttc      480

actgaaggga acatagcagc tatgattgtc aataagacag tgcacaaaat gattttctcg      540

aggcaaggac aagggtaccg tcacatgaat ctgacttcta ctaataaata ttggacaagt      600

aacaacggaa cacaaacgaa tgacactgga tgcttcggcg ctcttcaaga atacaactcc      660

acgaagaatc aaacatgtgc tccgtccaaa ataccttcac cactgcccac aacccgctca      720

gagattaaac ccacaagcac cccaactgat gccaccgcac tcaacaccac acaacatctt      780

gtatatttca gaaagaaacg aagtatcctc tggagggaag gcgacatgtt cccttttctg      840

gacgggttaa taaatgctcc aattgatttt gatccagttc caaatacaaa gacgatcttt      900

gatgaatctt ctagttctgg tgcttcggct gaggaagatc aacatgcctc ccccaatatc      960

agtttaactt tatcctattt tcctaatata aatgaaaaca ctgcctactc tggagaaaat     1020

gagaacgatt gtgatgcaga gttaagaatt tggagcgttc aggaggatga cctggcagca     1080

gggctcagtt ggataccgtt ttttggccct ggaatcgaag gactttatac tgctggttta     1140

attaaaaacc aaaacaattt ggtctgcagg ttgaggcgtc tagccaatca aactgccaaa     1200

tccttggaac tcttattaag agtcacaacc gaggagagga cattttcctt aattaataga     1260

catgccattg attttctact cacaaggtgg ggaggaacat gcaaagtgct tggacctgat     1320

tgttgcatcg gaatagaaga cttgtccagg aatatttcag agcaaattga ccaaatcaaa     1380

aaagatgaac aaaaagaggg gactggttgg ggtctaggtg gtaaatggtg gacatccgac     1440

tggggtgttc ttactaactt gggcattttg ctactattat ccatagctgt cttgattgct     1500

ctatcctgta tttgtcgtat ctttaccaaa tatatcgggt aa                        1542


