                         SEQUENCE LISTING

<110>  University of Cape Town
 
<120>  Fusion Proteins for the Detection of Apoptosis

<130>  PA168600/P

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  549
<212>  PRT
<213>  Artificial sequence

<220>
<223>  First Generation Annexin A5 SNAP polypeptide precursor amino acid
       sequence

<400>  1

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Ala Ala His His His His His His Ala Ala Asp 
            20                  25                  30          


Asp Asp Asp Lys Ala Ala Gln Pro Ala Ala Gln Val Leu Arg Gly Thr 
        35                  40                  45              


Val Thr Asp Phe Pro Gly Phe Asp Glu Arg Ala Asp Ala Glu Thr Leu 
    50                  55                  60                  


Arg Lys Ala Met Lys Gly Leu Gly Thr Asp Glu Glu Ser Ile Leu Thr 
65                  70                  75                  80  


Leu Leu Thr Ser Arg Ser Asn Ala Gln Arg Gln Glu Ile Ser Ala Ala 
                85                  90                  95      


Phe Lys Thr Leu Phe Gly Arg Asp Leu Leu Asp Asp Leu Lys Ser Glu 
            100                 105                 110         


Leu Thr Gly Lys Phe Glu Lys Leu Ile Val Ala Leu Met Lys Pro Ser 
        115                 120                 125             


Arg Leu Tyr Asp Ala Tyr Glu Leu Lys His Ala Leu Lys Gly Ala Gly 
    130                 135                 140                 


Thr Asn Glu Lys Val Leu Thr Glu Ile Ile Ala Ser Arg Thr Pro Glu 
145                 150                 155                 160 


Glu Leu Arg Ala Ile Lys Gln Val Tyr Glu Glu Glu Tyr Gly Ser Ser 
                165                 170                 175     


Leu Glu Asp Asp Val Val Gly Asp Thr Ser Gly Tyr Tyr Gln Arg Met 
            180                 185                 190         


Leu Val Val Leu Leu Gln Ala Asn Arg Asp Pro Asp Ala Gly Ile Asp 
        195                 200                 205             


Glu Ala Gln Val Glu Gln Asp Ala Gln Ala Leu Phe Gln Ala Gly Glu 
    210                 215                 220                 


Leu Lys Trp Gly Thr Asp Glu Glu Lys Phe Ile Thr Ile Phe Gly Thr 
225                 230                 235                 240 


Arg Ser Val Ser His Leu Arg Lys Val Phe Asp Lys Tyr Met Thr Ile 
                245                 250                 255     


Ser Gly Phe Gln Ile Glu Glu Thr Ile Asp Arg Glu Thr Ser Gly Asn 
            260                 265                 270         


Leu Glu Gln Leu Leu Leu Ala Val Val Lys Ser Ile Arg Ser Ile Pro 
        275                 280                 285             


Ala Tyr Leu Ala Glu Thr Leu Tyr Tyr Ala Met Lys Gly Ala Gly Thr 
    290                 295                 300                 


Asp Asp His Thr Leu Ile Arg Val Met Val Ser Arg Ser Glu Ile Asp 
305                 310                 315                 320 


Leu Phe Asn Ile Arg Lys Glu Phe Arg Lys Asn Phe Ala Thr Ser Leu 
                325                 330                 335     


Tyr Ser Met Ile Lys Gly Asp Thr Ser Gly Asp Tyr Lys Lys Ala Leu 
            340                 345                 350         


Leu Leu Leu Cys Gly Glu Asp Asp Ala Ala Ala Ser Arg Met Asp Lys 
        355                 360                 365             


Asp Cys Glu Met Lys Arg Thr Thr Leu Asp Ser Pro Leu Gly Lys Leu 
    370                 375                 380                 


Glu Leu Ser Gly Cys Glu Gln Gly Leu His Glu Ile Lys Leu Leu Gly 
385                 390                 395                 400 


Lys Gly Thr Ser Ala Ala Asp Ala Val Glu Val Pro Ala Pro Ala Ala 
                405                 410                 415     


Val Leu Gly Gly Pro Glu Pro Leu Met Gln Ala Thr Ala Trp Leu Asn 
            420                 425                 430         


Ala Tyr Phe His Gln Pro Glu Ala Ile Glu Glu Phe Pro Val Pro Ala 
        435                 440                 445             


Leu His His Pro Val Phe Gln Gln Glu Ser Phe Thr Arg Gln Val Leu 
    450                 455                 460                 


Trp Lys Leu Leu Lys Val Val Lys Phe Gly Glu Val Ile Ser Tyr Gln 
465                 470                 475                 480 


Gln Leu Ala Ala Leu Ala Gly Asn Pro Ala Ala Thr Ala Ala Val Lys 
                485                 490                 495     


Thr Ala Leu Ser Gly Asn Pro Val Pro Ile Leu Ile Pro Cys His Arg 
            500                 505                 510         


Val Val Ser Ser Ser Gly Ala Val Gly Gly Tyr Glu Gly Gly Leu Ala 
        515                 520                 525             


Val Lys Glu Trp Leu Leu Ala His Glu Gly His Arg Leu Gly Lys Pro 
    530                 535                 540                 


Gly Leu Ala Glu His 
545                 


<210>  2
<211>  1650
<212>  DNA
<213>  Artificial sequence

<220>
<223>  First Generation Annexin A5 SNAP polypeptide precursor nucleotide
       sequence

<400>  2
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt       60

gacgccgccc atcatcatca tcatcatgcc gccgacgacg acgacaaggc ggcccagccg      120

gccgcacagg ttctcagagg cactgtgact gacttccctg gatttgatga gcgggctgat      180

gcagaaactc ttcggaaggc tatgaaaggc ttgggcacag atgaggagag catcctgact      240

ctgttgacat cccgaagtaa tgcccagcgc caggaaatct ctgcagcttt taagactctg      300

tttggcaggg atcttctgga tgacctgaaa tcagaactaa ctggaaaatt tgaaaaatta      360

attgtggctc tgatgaaacc ctctcggctt tatgatgctt atgaactgaa acatgccttg      420

aagggagctg gaacaaatga aaaagtactg acagaaatta ttgcttcaag gacacctgaa      480

gaactgagag ccatcaaaca agtttatgaa gaagaatatg gctcaagcct ggaagatgac      540

gtggtggggg acacttcagg gtactaccag cggatgttgg tggttctcct tcaggctaac      600

agagaccctg atgctggaat tgatgaagct caagttgaac aagatgctca ggctttattt      660

caggctggag aacttaaatg ggggacagat gaagaaaagt ttatcaccat ctttggaaca      720

cgaagtgtgt ctcatttgag aaaggtgttt gacaagtaca tgactatatc aggatttcaa      780

attgaggaaa ccattgaccg cgagacttct ggcaatttag agcaactact ccttgctgtt      840

gtgaaatcta ttcgaagtat acctgcctac cttgcagaga ccctctatta tgctatgaag      900

ggagctggga cagatgatca taccctcatc agagtcatgg tttccaggag tgagattgat      960

ctgtttaaca tcaggaagga gtttaggaag aattttgcca cctctcttta ttccatgatt     1020

aagggagata catctgggga ctataagaaa gctcttctgc tgctctgtgg agaagatgac     1080

gcggccgctt ctagaatgga caaagactgc gaaatgaagc gcaccaccct ggatagccct     1140

ctgggcaagc tggaactgtc tgggtgcgaa cagggcctgc acgagatcaa gctgctgggc     1200

aaaggaacat ctgccgccga cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga     1260

ccagagccac tgatgcaggc caccgcctgg ctcaacgcct actttcacca gcctgaggcc     1320

atcgaggagt tccctgtgcc agccctgcac cacccagtgt tccagcagga gagctttacc     1380

cgccaggtgc tgtggaaact gctgaaagtg gtgaagttcg gagaggtcat cagctaccag     1440

cagctggcgg ccctggcggg caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc     1500

ggaaatcccg tgcccattct gatcccctgc caccgggtgg tgtctagctc tggcgccgtg     1560

gggggctacg agggcgggct cgccgtgaaa gagtggctgc tggcccacga gggccacaga     1620

ctgggcaagc ctgggctggc tgagcactga                                      1650


<210>  3
<211>  513
<212>  PRT
<213>  Artificial sequence

<220>
<223>  First Generation Annexin A5 SNAP mature polypeptide amino acid 
       sequence

<400>  3

Ala Ala Gln Pro Ala Ala Gln Val Leu Arg Gly Thr Val Thr Asp Phe 
1               5                   10                  15      


Pro Gly Phe Asp Glu Arg Ala Asp Ala Glu Thr Leu Arg Lys Ala Met 
            20                  25                  30          


Lys Gly Leu Gly Thr Asp Glu Glu Ser Ile Leu Thr Leu Leu Thr Ser 
        35                  40                  45              


Arg Ser Asn Ala Gln Arg Gln Glu Ile Ser Ala Ala Phe Lys Thr Leu 
    50                  55                  60                  


Phe Gly Arg Asp Leu Leu Asp Asp Leu Lys Ser Glu Leu Thr Gly Lys 
65                  70                  75                  80  


Phe Glu Lys Leu Ile Val Ala Leu Met Lys Pro Ser Arg Leu Tyr Asp 
                85                  90                  95      


Ala Tyr Glu Leu Lys His Ala Leu Lys Gly Ala Gly Thr Asn Glu Lys 
            100                 105                 110         


Val Leu Thr Glu Ile Ile Ala Ser Arg Thr Pro Glu Glu Leu Arg Ala 
        115                 120                 125             


Ile Lys Gln Val Tyr Glu Glu Glu Tyr Gly Ser Ser Leu Glu Asp Asp 
    130                 135                 140                 


Val Val Gly Asp Thr Ser Gly Tyr Tyr Gln Arg Met Leu Val Val Leu 
145                 150                 155                 160 


Leu Gln Ala Asn Arg Asp Pro Asp Ala Gly Ile Asp Glu Ala Gln Val 
                165                 170                 175     


Glu Gln Asp Ala Gln Ala Leu Phe Gln Ala Gly Glu Leu Lys Trp Gly 
            180                 185                 190         


Thr Asp Glu Glu Lys Phe Ile Thr Ile Phe Gly Thr Arg Ser Val Ser 
        195                 200                 205             


His Leu Arg Lys Val Phe Asp Lys Tyr Met Thr Ile Ser Gly Phe Gln 
    210                 215                 220                 


Ile Glu Glu Thr Ile Asp Arg Glu Thr Ser Gly Asn Leu Glu Gln Leu 
225                 230                 235                 240 


Leu Leu Ala Val Val Lys Ser Ile Arg Ser Ile Pro Ala Tyr Leu Ala 
                245                 250                 255     


Glu Thr Leu Tyr Tyr Ala Met Lys Gly Ala Gly Thr Asp Asp His Thr 
            260                 265                 270         


Leu Ile Arg Val Met Val Ser Arg Ser Glu Ile Asp Leu Phe Asn Ile 
        275                 280                 285             


Arg Lys Glu Phe Arg Lys Asn Phe Ala Thr Ser Leu Tyr Ser Met Ile 
    290                 295                 300                 


Lys Gly Asp Thr Ser Gly Asp Tyr Lys Lys Ala Leu Leu Leu Leu Cys 
305                 310                 315                 320 


Gly Glu Asp Asp Ala Ala Ala Ser Arg Met Asp Lys Asp Cys Glu Met 
                325                 330                 335     


Lys Arg Thr Thr Leu Asp Ser Pro Leu Gly Lys Leu Glu Leu Ser Gly 
            340                 345                 350         


Cys Glu Gln Gly Leu His Glu Ile Lys Leu Leu Gly Lys Gly Thr Ser 
        355                 360                 365             


Ala Ala Asp Ala Val Glu Val Pro Ala Pro Ala Ala Val Leu Gly Gly 
    370                 375                 380                 


Pro Glu Pro Leu Met Gln Ala Thr Ala Trp Leu Asn Ala Tyr Phe His 
385                 390                 395                 400 


Gln Pro Glu Ala Ile Glu Glu Phe Pro Val Pro Ala Leu His His Pro 
                405                 410                 415     


Val Phe Gln Gln Glu Ser Phe Thr Arg Gln Val Leu Trp Lys Leu Leu 
            420                 425                 430         


Lys Val Val Lys Phe Gly Glu Val Ile Ser Tyr Gln Gln Leu Ala Ala 
        435                 440                 445             


Leu Ala Gly Asn Pro Ala Ala Thr Ala Ala Val Lys Thr Ala Leu Ser 
    450                 455                 460                 


Gly Asn Pro Val Pro Ile Leu Ile Pro Cys His Arg Val Val Ser Ser 
465                 470                 475                 480 


Ser Gly Ala Val Gly Gly Tyr Glu Gly Gly Leu Ala Val Lys Glu Trp 
                485                 490                 495     


Leu Leu Ala His Glu Gly His Arg Leu Gly Lys Pro Gly Leu Ala Glu 
            500                 505                 510         


His 
    


<210>  4
<211>  978
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Modified Annexin A5 ORF nucleotide sequence with restriction 
       sites

<400>  4
ggcccagccg gccgcacagg ttctcagagg cactgtgact gacttccctg gatttgatga       60

gcgggctgat gcagaaactc ttcggaaggc tatgaaaggc ttgggcacag atgaggagag      120

catcctgact ctgttgacat cccgaagtaa tgcccagcgc caggaaatct ctgcagcttt      180

taagactctg tttggcaggg atcttctgga tgacctgaaa tcagaactaa ctggaaaatt      240

tgaaaaatta attgtggctc tgatgaaacc ctctcggctt tatgatgctt atgaactgaa      300

acatgccttg aagggagctg gaacaaatga aaaagtactg acagaaatta ttgcttcaag      360

gacacctgaa gaactgagag ccatcaaaca agtttatgaa gaagaatatg gctcaagcct      420

ggaagatgac gtggtggggg acacttcagg gtactaccag cggatgttgg tggttctcct      480

tcaggctaac agagaccctg atgctggaat tgatgaagct caagttgaac aagatgctca      540

ggctttattt caggctggag aacttaaatg ggggacagat gaagaaaagt ttatcaccat      600

ctttggaaca cgaagtgtgt ctcatttgag aaaggtgttt gacaagtaca tgactatatc      660

aggatttcaa attgaggaaa ccattgaccg cgagacttct ggcaatttag agcaactact      720

ccttgctgtt gtgaaatcta ttcgaagtat acctgcctac cttgcagaga ccctctatta      780

tgctatgaag ggagctggga cagatgatca taccctcatc agagtcatgg tttccaggag      840

tgagattgat ctgtttaaca tcaggaagga gtttaggaag aattttgcca cctctcttta      900

ttccatgatt aagggagata catctgggga ctataagaaa gctcttctgc tgctctgtgg      960

agaagatgac gcggccgc                                                    978


<210>  5
<211>  319
<212>  PRT
<213>  Homo sapiens

<400>  5

Ala Gln Val Leu Arg Gly Thr Val Thr Asp Phe Pro Gly Phe Asp Glu 
1               5                   10                  15      


Arg Ala Asp Ala Glu Thr Leu Arg Lys Ala Met Lys Gly Leu Gly Thr 
            20                  25                  30          


Asp Glu Glu Ser Ile Leu Thr Leu Leu Thr Ser Arg Ser Asn Ala Gln 
        35                  40                  45              


Arg Gln Glu Ile Ser Ala Ala Phe Lys Thr Leu Phe Gly Arg Asp Leu 
    50                  55                  60                  


Leu Asp Asp Leu Lys Ser Glu Leu Thr Gly Lys Phe Glu Lys Leu Ile 
65                  70                  75                  80  


Val Ala Leu Met Lys Pro Ser Arg Leu Tyr Asp Ala Tyr Glu Leu Lys 
                85                  90                  95      


His Ala Leu Lys Gly Ala Gly Thr Asn Glu Lys Val Leu Thr Glu Ile 
            100                 105                 110         


Ile Ala Ser Arg Thr Pro Glu Glu Leu Arg Ala Ile Lys Gln Val Tyr 
        115                 120                 125             


Glu Glu Glu Tyr Gly Ser Ser Leu Glu Asp Asp Val Val Gly Asp Thr 
    130                 135                 140                 


Ser Gly Tyr Tyr Gln Arg Met Leu Val Val Leu Leu Gln Ala Asn Arg 
145                 150                 155                 160 


Asp Pro Asp Ala Gly Ile Asp Glu Ala Gln Val Glu Gln Asp Ala Gln 
                165                 170                 175     


Ala Leu Phe Gln Ala Gly Glu Leu Lys Trp Gly Thr Asp Glu Glu Lys 
            180                 185                 190         


Phe Ile Thr Ile Phe Gly Thr Arg Ser Val Ser His Leu Arg Lys Val 
        195                 200                 205             


Phe Asp Lys Tyr Met Thr Ile Ser Gly Phe Gln Ile Glu Glu Thr Ile 
    210                 215                 220                 


Asp Arg Glu Thr Ser Gly Asn Leu Glu Gln Leu Leu Leu Ala Val Val 
225                 230                 235                 240 


Lys Ser Ile Arg Ser Ile Pro Ala Tyr Leu Ala Glu Thr Leu Tyr Tyr 
                245                 250                 255     


Ala Met Lys Gly Ala Gly Thr Asp Asp His Thr Leu Ile Arg Val Met 
            260                 265                 270         


Val Ser Arg Ser Glu Ile Asp Leu Phe Asn Ile Arg Lys Glu Phe Arg 
        275                 280                 285             


Lys Asn Phe Ala Thr Ser Leu Tyr Ser Met Ile Lys Gly Asp Thr Ser 
    290                 295                 300                 


Gly Asp Tyr Lys Lys Ala Leu Leu Leu Leu Cys Gly Glu Asp Asp 
305                 310                 315                 


<210>  6
<211>  543
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SNAP-tag nucleotide sequence

<400>  6
atggacaaag actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa       60

ctgtctgggt gcgaacaggg cctgcacgag atcaagctgc tgggcaaagg aacatctgcc      120

gccgacgccg tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatg      180

caggccaccg cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct      240

gtgccagccc tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg      300

aaactgctga aagtggtgaa gttcggagag gtcatcagct accagcagct ggcggccctg      360

gcgggcaatc ccgccgccac cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc      420

attctgatcc cctgccaccg ggtggtgtct agctctggcg ccgtgggggg ctacgagggc      480

gggctcgccg tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg      540

ctg                                                                    543


<210>  7
<211>  181
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SNAP-tag amino acid sequence

<400>  7

Met Asp Lys Asp Cys Glu Met Lys Arg Thr Thr Leu Asp Ser Pro Leu 
1               5                   10                  15      


Gly Lys Leu Glu Leu Ser Gly Cys Glu Gln Gly Leu His Glu Ile Lys 
            20                  25                  30          


Leu Leu Gly Lys Gly Thr Ser Ala Ala Asp Ala Val Glu Val Pro Ala 
        35                  40                  45              


Pro Ala Ala Val Leu Gly Gly Pro Glu Pro Leu Met Gln Ala Thr Ala 
    50                  55                  60                  


Trp Leu Asn Ala Tyr Phe His Gln Pro Glu Ala Ile Glu Glu Phe Pro 
65                  70                  75                  80  


Val Pro Ala Leu His His Pro Val Phe Gln Gln Glu Ser Phe Thr Arg 
                85                  90                  95      


Gln Val Leu Trp Lys Leu Leu Lys Val Val Lys Phe Gly Glu Val Ile 
            100                 105                 110         


Ser Tyr Gln Gln Leu Ala Ala Leu Ala Gly Asn Pro Ala Ala Thr Ala 
        115                 120                 125             


Ala Val Lys Thr Ala Leu Ser Gly Asn Pro Val Pro Ile Leu Ile Pro 
    130                 135                 140                 


Cys His Arg Val Val Ser Ser Ser Gly Ala Val Gly Gly Tyr Glu Gly 
145                 150                 155                 160 


Gly Leu Ala Val Lys Glu Trp Leu Leu Ala His Glu Gly His Arg Leu 
                165                 170                 175     


Gly Lys Pro Gly Leu 
            180     


<210>  8
<211>  63
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Ig-Kappa leader nucleotide sequence

<400>  8
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt       60

gac                                                                     63


<210>  9
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Ig-Kappa leader amino acid sequence

<400>  9

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp 
            20      


<210>  10
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  6xHis tag nucleotide sequence

<400>  10
catcatcatc atcatcat                                                     18


<210>  11
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  6xHis tag amino acid sequence

<400>  11

His His His His His His 
1               5       


<210>  12
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Enterokinase cleavage site nucleotide sequence

<400>  12
gacgacgacg acaag                                                        15


<210>  13
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Enterokinase cleavage site amino acid sequence

<400>  13

Asp Asp Asp Asp Lys 
1               5   


<210>  14
<211>  551
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Second generation Annexin A5 SNAP polypeptide precursor amino 
       acid sequence

<400>  14

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Ala Ala His His His His His His Ala Ala Asp 
            20                  25                  30          


Asp Asp Asp Lys Ala Ala Gln Pro Ala Ala Gln Val Leu Arg Gly Thr 
        35                  40                  45              


Val Thr Asp Phe Pro Gly Phe Asp Glu Arg Ala Asp Ala Glu Thr Leu 
    50                  55                  60                  


Arg Lys Ala Met Lys Gly Leu Gly Thr Asp Glu Glu Ser Ile Leu Thr 
65                  70                  75                  80  


Leu Leu Thr Ser Arg Ser Asn Ala Gln Arg Gln Glu Ile Ser Ala Ala 
                85                  90                  95      


Phe Lys Thr Leu Phe Gly Arg Asp Leu Leu Asp Asp Leu Lys Ser Glu 
            100                 105                 110         


Leu Thr Gly Lys Phe Glu Lys Leu Ile Val Ala Leu Met Lys Pro Ser 
        115                 120                 125             


Arg Leu Tyr Asp Ala Tyr Glu Leu Lys His Ala Leu Lys Gly Ala Gly 
    130                 135                 140                 


Thr Asn Glu Lys Val Leu Thr Glu Ile Ile Ala Ser Arg Thr Pro Glu 
145                 150                 155                 160 


Glu Leu Arg Ala Ile Lys Gln Val Tyr Glu Glu Glu Tyr Gly Ser Ser 
                165                 170                 175     


Leu Glu Asp Asp Val Val Gly Asp Thr Ser Gly Tyr Tyr Gln Arg Met 
            180                 185                 190         


Leu Val Val Leu Leu Gln Ala Asn Arg Asp Pro Asp Ala Gly Ile Asp 
        195                 200                 205             


Glu Ala Gln Val Glu Gln Asp Ala Gln Ala Leu Phe Gln Ala Gly Glu 
    210                 215                 220                 


Leu Lys Trp Gly Thr Asp Glu Glu Lys Phe Ile Thr Ile Phe Gly Thr 
225                 230                 235                 240 


Arg Ser Val Ser His Leu Arg Lys Val Phe Asp Lys Tyr Met Thr Ile 
                245                 250                 255     


Ser Gly Phe Gln Ile Glu Glu Thr Ile Asp Arg Glu Thr Ser Gly Asn 
            260                 265                 270         


Leu Glu Gln Leu Leu Leu Ala Val Val Lys Ser Ile Arg Ser Ile Pro 
        275                 280                 285             


Ala Tyr Leu Ala Glu Thr Leu Tyr Tyr Ala Met Lys Gly Ala Gly Thr 
    290                 295                 300                 


Asp Asp His Thr Leu Ile Arg Val Met Val Ser Arg Ser Glu Ile Asp 
305                 310                 315                 320 


Leu Phe Asn Ile Arg Lys Glu Phe Arg Lys Asn Phe Ala Thr Ser Leu 
                325                 330                 335     


Tyr Ser Met Ile Lys Gly Asp Thr Ser Gly Asp Tyr Lys Lys Ala Leu 
            340                 345                 350         


Leu Leu Leu Cys Gly Glu Asp Asp Ala Ala Ala Leu Glu Ser Arg Met 
        355                 360                 365             


Asp Lys Asp Cys Glu Met Lys Arg Thr Thr Leu Asp Ser Pro Leu Gly 
    370                 375                 380                 


Lys Leu Glu Leu Ser Gly Cys Glu Gln Gly Leu His Glu Ile Lys Leu 
385                 390                 395                 400 


Leu Gly Lys Gly Thr Ser Ala Ala Asp Ala Val Glu Val Pro Ala Pro 
                405                 410                 415     


Ala Ala Val Leu Gly Gly Pro Glu Pro Leu Met Gln Ala Thr Ala Trp 
            420                 425                 430         


Leu Asn Ala Tyr Phe His Gln Pro Glu Ala Ile Glu Glu Phe Pro Val 
        435                 440                 445             


Pro Ala Leu His His Pro Val Phe Gln Gln Glu Ser Phe Thr Arg Gln 
    450                 455                 460                 


Val Leu Trp Lys Leu Leu Lys Val Val Lys Phe Gly Glu Val Ile Ser 
465                 470                 475                 480 


Tyr Gln Gln Leu Ala Ala Leu Ala Gly Asn Pro Ala Ala Thr Ala Ala 
                485                 490                 495     


Val Lys Thr Ala Leu Ser Gly Asn Pro Val Pro Ile Leu Ile Pro Cys 
            500                 505                 510         


His Arg Val Val Ser Ser Ser Gly Ala Val Gly Gly Tyr Glu Gly Gly 
        515                 520                 525             


Leu Ala Val Lys Glu Trp Leu Leu Ala His Glu Gly His Arg Leu Gly 
    530                 535                 540                 


Lys Pro Gly Leu Ala Glu His 
545                 550     


<210>  15
<211>  1656
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Second generation Annexin A5 SNAP polypeptide precursor 
       nucleotide sequence

<400>  15
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt       60

gacgccgccc atcatcatca tcatcatgcc gccgacgacg acgacaaggc ggcccagccg      120

gccgcacagg ttctcagagg cactgtgact gacttccctg gatttgatga gcgggctgat      180

gcagaaactc ttcggaaggc tatgaaaggc ttgggcacag atgaggagag catcctgact      240

ctgttgacat cccgaagtaa tgcccagcgc caggaaatct ctgcagcttt taagactctg      300

tttggcaggg atcttctgga tgacctgaaa tcagaactaa ctggaaaatt tgaaaaatta      360

attgtggctc tgatgaaacc ctctcggctt tatgatgctt atgaactgaa acatgccttg      420

aagggagctg gaacaaatga aaaagtactg acagaaatta ttgcttcaag gacacctgaa      480

gaactgagag ccatcaaaca agtttatgaa gaagaatatg gctcaagcct ggaagatgac      540

gtggtggggg acacttcagg gtactaccag cggatgttgg tggttctcct tcaggctaac      600

agagaccctg atgctggaat tgatgaagct caagttgaac aagatgctca ggctttattt      660

caggctggag aacttaaatg ggggacagat gaagaaaagt ttatcaccat ctttggaaca      720

cgaagtgtgt ctcatttgag aaaggtgttt gacaagtaca tgactatatc aggatttcaa      780

attgaggaaa ccattgaccg cgagacttct ggcaatttag agcaactact ccttgctgtt      840

gtgaaatcta ttcgaagtat acctgcctac cttgcagaga ccctctatta tgctatgaag      900

ggagctggga cagatgatca taccctcatc agagtcatgg tttccaggag tgagattgat      960

ctgtttaaca tcaggaagga gtttaggaag aattttgcca cctctcttta ttccatgatt     1020

aagggagata catctgggga ctataagaaa gctcttctgc tgctctgtgg agaagatgac     1080

gcggccgcac tcgagtctag aatggacaaa gactgcgaaa tgaagcgcac caccctggat     1140

agccctctgg gcaagctgga actgtctggg tgcgaacagg gcctgcacga gatcaagctg     1200

ctgggcaaag gaacatctgc cgccgacgcc gtggaagtgc ctgccccagc cgccgtgctg     1260

ggcggaccag agccactgat gcaggccacc gcctggctca acgcctactt tcaccagcct     1320

gaggccatcg aggagttccc tgtgccagcc ctgcaccacc cagtgttcca gcaggagagc     1380

tttacccgcc aggtgctgtg gaaactgctg aaagtggtga agttcggaga ggtcatcagc     1440

taccagcagc tggcggccct ggcgggcaat cccgccgcca ccgccgccgt gaaaaccgcc     1500

ctgagcggaa atcccgtgcc cattctgatc ccctgccacc gggtggtgtc tagctctggc     1560

gccgtggggg gctacgaggg cgggctcgcc gtgaaagagt ggctgctggc ccacgagggc     1620

cacagactgg gcaagcctgg gctggctgag cactga                               1656


<210>  16
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Second Generation Linker Peptide

<400>  16

Ala Ala Ala Leu Glu Ser Arg 
1               5           


