SEQUENCE LISTING

<110> Cellectis

<120> REPROGRAMMING OF GENETICALLY ENGINEERED PRIMARY IMMUNE CELLS

<130> P81704794PCT00 

<150> PA201770906
<151> 2017-12-01

<160> 15

<170> PatentIn version 3.5

 
<210>  1

<211>  925
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN DCK-R

<400>  1
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  2

<211>  925
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN DCK-L

<400>  2
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Ile Ala Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
        275                 280                 285             


Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
305                 310                 315                 320 


Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
            340                 345                 350         


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro 
545                 550                 555                 560 


Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
            580                 585                 590         


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
        595                 600                 605             


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
    610                 615                 620                 


Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly 
625                 630                 635                 640 


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
                645                 650                 655     


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
            660                 665                 670         


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
        675                 680                 685             


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
    690                 695                 700                 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
705                 710                 715                 720 


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
                725                 730                 735     


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
            740                 745                 750         


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
        755                 760                 765             


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
    770                 775                 780                 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
785                 790                 795                 800 


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
                805                 810                 815     


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
            820                 825                 830         


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
        835                 840                 845             


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
    850                 855                 860                 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
865                 870                 875                 880 


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
                885                 890                 895     


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
            900                 905                 910         


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
        915                 920                 925 


<210>  3

<211>  936
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN TRAC-R

<400>  3
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Tyr Pro Tyr Asp 
1               5                   10                  15      


Val Pro Asp Tyr Ala Ile Asp Ile Ala Asp Leu Arg Thr Leu Gly Tyr 
            20                  25                  30          


Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val 
        35                  40                  45              


Ala Gln His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His 
    50                  55                  60                  


Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val 
65                  70                  75                  80  


Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu Ala Thr His Glu Ala 
                85                  90                  95      


Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala 
            100                 105                 110         


Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp 
        115                 120                 125             


Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Val 
    130                 135                 140                 


Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn 
145                 150                 155                 160 


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
                165                 170                 175     


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
            180                 185                 190         


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
        195                 200                 205             


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    210                 215                 220                 


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
225                 230                 235                 240 


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                245                 250                 255     


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
            260                 265                 270         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        275                 280                 285             


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
    290                 295                 300                 


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
305                 310                 315                 320 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
                325                 330                 335     


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
            340                 345                 350         


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
        355                 360                 365             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    370                 375                 380                 


Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
385                 390                 395                 400 


Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                405                 410                 415     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
            420                 425                 430         


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
        435                 440                 445             


Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala 
    450                 455                 460                 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
465                 470                 475                 480 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                485                 490                 495     


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn 
            500                 505                 510         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val 
        515                 520                 525             


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
    530                 535                 540                 


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
545                 550                 555                 560 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
                565                 570                 575     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala 
            580                 585                 590         


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
        595                 600                 605             


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
    610                 615                 620                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
625                 630                 635                 640 


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
                645                 650                 655     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            660                 665                 670         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala 
        675                 680                 685             


Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala 
    690                 695                 700                 


Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg 
705                 710                 715                 720 


Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly Asp Pro Ile Ser Arg 
                725                 730                 735     


Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg 
            740                 745                 750         


His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile 
        755                 760                 765             


Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu 
    770                 775                 780                 


Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser 
785                 790                 795                 800 


Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr 
                805                 810                 815     


Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro 
            820                 825                 830         


Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Glu Glu Asn Gln Thr 
        835                 840                 845             


Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser 
    850                 855                 860                 


Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly 
865                 870                 875                 880 


Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His Ile Thr Asn Cys Asn 
                885                 890                 895     


Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile 
            900                 905                 910         


Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn 
        915                 920                 925             


Gly Glu Ile Asn Phe Ala Ala Asp 
    930                 935     


<210>  4

<211>  942
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN TRAC-L

<400>  4
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Lys Glu Thr Ala 
1               5                   10                  15      


Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser Ile Asp Ile Ala Asp 
            20                  25                  30          


Leu Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro 
        35                  40                  45              


Lys Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His 
    50                  55                  60                  


Gly Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala 
65                  70                  75                  80  


Leu Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro 
                85                  90                  95      


Glu Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly 
            100                 105                 110         


Ala Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly 
        115                 120                 125             


Pro Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg 
    130                 135                 140                 


Gly Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu 
145                 150                 155                 160 


Thr Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
                165                 170                 175     


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
            180                 185                 190         


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
        195                 200                 205             


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
    210                 215                 220                 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
225                 230                 235                 240 


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
                245                 250                 255     


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
            260                 265                 270         


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
        275                 280                 285             


Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
    290                 295                 300                 


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
305                 310                 315                 320 


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
                325                 330                 335     


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
            340                 345                 350         


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
        355                 360                 365             


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
    370                 375                 380                 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
385                 390                 395                 400 


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
                405                 410                 415     


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
            420                 425                 430         


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
        435                 440                 445             


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
    450                 455                 460                 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
465                 470                 475                 480 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
                485                 490                 495     


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
            500                 505                 510         


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
        515                 520                 525             


Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
    530                 535                 540                 


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
545                 550                 555                 560 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
                565                 570                 575     


Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala 
            580                 585                 590         


Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly 
        595                 600                 605             


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
    610                 615                 620                 


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
625                 630                 635                 640 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
                645                 650                 655     


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
            660                 665                 670         


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
        675                 680                 685             


Gly Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg 
    690                 695                 700                 


Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu 
705                 710                 715                 720 


Ala Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu 
                725                 730                 735     


Gly Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu 
            740                 745                 750         


Lys Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr 
        755                 760                 765             


Ile Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu 
    770                 775                 780                 


Glu Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly 
785                 790                 795                 800 


Lys His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val 
                805                 810                 815     


Gly Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser 
            820                 825                 830         


Gly Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr 
        835                 840                 845             


Val Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp 
    850                 855                 860                 


Trp Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val 
865                 870                 875                 880 


Ser Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn 
                885                 890                 895     


His Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu 
            900                 905                 910         


Ile Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val 
        915                 920                 925             


Arg Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
    930                 935                 940         


<210>  5

<211>  913
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN CD25-R

<400>  5
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Tyr Pro Tyr Asp 
1               5                   10                  15      


Val Pro Asp Tyr Ala Ile Asp Ile Ala Asp Leu Arg Thr Leu Gly Tyr 
            20                  25                  30          


Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val 
        35                  40                  45              


Ala Gln His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His 
    50                  55                  60                  


Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val 
65                  70                  75                  80  


Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu Ala Thr His Glu Ala 
                85                  90                  95      


Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala 
            100                 105                 110         


Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp 
        115                 120                 125             


Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Val 
    130                 135                 140                 


Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn 
145                 150                 155                 160 


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
                165                 170                 175     


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
            180                 185                 190         


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly 
        195                 200                 205             


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    210                 215                 220                 


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
225                 230                 235                 240 


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                245                 250                 255     


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
            260                 265                 270         


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        275                 280                 285             


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
    290                 295                 300                 


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
305                 310                 315                 320 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
                325                 330                 335     


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
            340                 345                 350         


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
        355                 360                 365             


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
    370                 375                 380                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
385                 390                 395                 400 


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala 
                405                 410                 415     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
            420                 425                 430         


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        435                 440                 445             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
    450                 455                 460                 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
465                 470                 475                 480 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                485                 490                 495     


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
            500                 505                 510         


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        515                 520                 525             


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
    530                 535                 540                 


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
545                 550                 555                 560 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
                565                 570                 575     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            580                 585                 590         


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
        595                 600                 605             


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
    610                 615                 620                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
625                 630                 635                 640 


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
                645                 650                 655     


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            660                 665                 670         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala 
        675                 680                 685             


Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ser Gly Ser 
    690                 695                 700                 


Gly Ser Gly Gly Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu 
705                 710                 715                 720 


Leu Glu Glu Lys Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro 
                725                 730                 735     


His Glu Tyr Ile Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp 
            740                 745                 750         


Arg Ile Leu Glu Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly 
        755                 760                 765             


Tyr Arg Gly Lys His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile 
    770                 775                 780                 


Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys 
785                 790                 795                 800 


Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met 
                805                 810                 815     


Gln Arg Tyr Val Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro 
            820                 825                 830         


Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe 
        835                 840                 845             


Leu Phe Val Ser Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr 
    850                 855                 860                 


Arg Leu Asn His Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu 
865                 870                 875                 880 


Glu Leu Leu Ile Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu 
                885                 890                 895     


Glu Glu Val Arg Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala 
            900                 905                 910         


Asp 
    


<210>  6

<211>  913
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN CD25-L

<400>  6
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Tyr Pro Tyr Asp 
1               5                   10                  15      


Val Pro Asp Tyr Ala Ile Asp Ile Ala Asp Leu Arg Thr Leu Gly Tyr 
            20                  25                  30          


Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val 
        35                  40                  45              


Ala Gln His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His 
    50                  55                  60                  


Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val 
65                  70                  75                  80  


Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu Ala Thr His Glu Ala 
                85                  90                  95      


Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala 
            100                 105                 110         


Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp 
        115                 120                 125             


Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Val 
    130                 135                 140                 


Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn 
145                 150                 155                 160 


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
                165                 170                 175     


Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala 
            180                 185                 190         


His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly 
        195                 200                 205             


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    210                 215                 220                 


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn 
225                 230                 235                 240 


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val 
                245                 250                 255     


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
            260                 265                 270         


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        275                 280                 285             


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
    290                 295                 300                 


Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
305                 310                 315                 320 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
                325                 330                 335     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            340                 345                 350         


Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
        355                 360                 365             


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
    370                 375                 380                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
385                 390                 395                 400 


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala 
                405                 410                 415     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
            420                 425                 430         


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
        435                 440                 445             


Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala 
    450                 455                 460                 


His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn Ile Gly 
465                 470                 475                 480 


Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys 
                485                 490                 495     


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
            500                 505                 510         


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        515                 520                 525             


Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
    530                 535                 540                 


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu 
545                 550                 555                 560 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
                565                 570                 575     


Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            580                 585                 590         


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
        595                 600                 605             


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
    610                 615                 620                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
625                 630                 635                 640 


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
                645                 650                 655     


Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            660                 665                 670         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala 
        675                 680                 685             


Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ser Gly Ser 
    690                 695                 700                 


Gly Ser Gly Gly Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu 
705                 710                 715                 720 


Leu Glu Glu Lys Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro 
                725                 730                 735     


His Glu Tyr Ile Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp 
            740                 745                 750         


Arg Ile Leu Glu Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly 
        755                 760                 765             


Tyr Arg Gly Lys His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile 
    770                 775                 780                 


Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys 
785                 790                 795                 800 


Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met 
                805                 810                 815     


Gln Arg Tyr Val Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro 
            820                 825                 830         


Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe 
        835                 840                 845             


Leu Phe Val Ser Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr 
    850                 855                 860                 


Arg Leu Asn His Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu 
865                 870                 875                 880 


Glu Leu Leu Ile Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu 
                885                 890                 895     


Glu Glu Val Arg Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala 
            900                 905                 910         


Asp 
    


<210>  7

<211>  936
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN PD1-R

<400>  7
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Tyr Pro Tyr Asp 
1               5                   10                  15      


Val Pro Asp Tyr Ala Ile Asp Ile Ala Asp Leu Arg Thr Leu Gly Tyr 
            20                  25                  30          


Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val 
        35                  40                  45              


Ala Gln His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His 
    50                  55                  60                  


Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val 
65                  70                  75                  80  


Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu Ala Thr His Glu Ala 
                85                  90                  95      


Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala 
            100                 105                 110         


Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp 
        115                 120                 125             


Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Val 
    130                 135                 140                 


Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn 
145                 150                 155                 160 


Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Lys Leu Gly Gly Lys 
                165                 170                 175     


Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala 
            180                 185                 190         


His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly 
        195                 200                 205             


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
    210                 215                 220                 


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His 
225                 230                 235                 240 


Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                245                 250                 255     


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
            260                 265                 270         


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        275                 280                 285             


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
    290                 295                 300                 


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
305                 310                 315                 320 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
                325                 330                 335     


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
            340                 345                 350         


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
        355                 360                 365             


Gln Val Val Ala Ile Ala Ser Tyr Lys Gly Gly Lys Gln Ala Leu Glu 
    370                 375                 380                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
385                 390                 395                 400 


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala 
                405                 410                 415     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
            420                 425                 430         


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys 
        435                 440                 445             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
    450                 455                 460                 


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
465                 470                 475                 480 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                485                 490                 495     


Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn 
            500                 505                 510         


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        515                 520                 525             


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
    530                 535                 540                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
545                 550                 555                 560 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
                565                 570                 575     


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            580                 585                 590         


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
        595                 600                 605             


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
    610                 615                 620                 


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu 
625                 630                 635                 640 


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
                645                 650                 655     


Thr Val Gln Ala Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
            660                 665                 670         


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Arg Pro Ala 
        675                 680                 685             


Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala 
    690                 695                 700                 


Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg 
705                 710                 715                 720 


Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly Asp Pro Ile Ser Arg 
                725                 730                 735     


Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg 
            740                 745                 750         


His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile 
        755                 760                 765             


Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu 
    770                 775                 780                 


Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser 
785                 790                 795                 800 


Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr 
                805                 810                 815     


Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro 
            820                 825                 830         


Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Glu Glu Asn Gln Thr 
        835                 840                 845             


Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser 
    850                 855                 860                 


Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly 
865                 870                 875                 880 


Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His Ile Thr Asn Cys Asn 
                885                 890                 895     


Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile 
            900                 905                 910         


Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn 
        915                 920                 925             


Gly Glu Ile Asn Phe Ala Ala Asp 
    930                 935     


<210>  8

<211>  941
<212>  PRT
<213>  artificial sequence

<220>
<223>  TALEN PD1-L

<400>  8
Met Gly Asp Pro Lys Lys Lys Arg Lys Val Ile Asp Lys Glu Thr Ala 
1               5                   10                  15      


Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser Ile Asp Ile Ala Asp 
            20                  25                  30          


Leu Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro 
        35                  40                  45              


Lys Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His 
    50                  55                  60                  


Gly Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala 
65                  70                  75                  80  


Leu Gly Thr Val Ala Val Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro 
                85                  90                  95      


Glu Ala Thr His Glu Ala Ile Val Gly Val Gly Lys Gln Trp Ser Gly 
            100                 105                 110         


Ala Arg Ala Leu Glu Ala Leu Leu Thr Val Ala Gly Glu Leu Arg Gly 
        115                 120                 125             


Pro Pro Leu Gln Leu Asp Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg 
    130                 135                 140                 


Gly Gly Val Thr Ala Val Glu Ala Val His Ala Trp Arg Asn Ala Leu 
145                 150                 155                 160 


Thr Gly Ala Pro Leu Asn Leu Thr Pro Glu Gln Val Val Ala Ile Ala 
                165                 170                 175     


Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
            180                 185                 190         


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala 
        195                 200                 205             


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
    210                 215                 220                 


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val 
225                 230                 235                 240 


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val 
                245                 250                 255     


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
            260                 265                 270         


Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu 
        275                 280                 285             


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
    290                 295                 300                 


Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala 
305                 310                 315                 320 


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly 
                325                 330                 335     


Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
            340                 345                 350         


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala 
        355                 360                 365             


His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
    370                 375                 380                 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
385                 390                 395                 400 


Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser Asn 
                405                 410                 415     


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Ala Leu Leu Pro Val 
            420                 425                 430         


Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala 
        435                 440                 445             


Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
    450                 455                 460                 


Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Glu Gln Val Val Ala 
465                 470                 475                 480 


Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
                485                 490                 495     


Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln Gln Val 
            500                 505                 510         


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
        515                 520                 525             


Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr Pro Gln 
    530                 535                 540                 


Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu 
545                 550                 555                 560 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu Thr 
                565                 570                 575     


Pro Glu Gln Val Val Ala Ile Ala Ser Asn Gly Gly Lys Gln Ala Leu 
            580                 585                 590         


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His Gly Leu 
        595                 600                 605             


Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
    610                 615                 620                 


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Ala His 
625                 630                 635                 640 


Gly Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly 
                645                 650                 655     


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
            660                 665                 670         


Ala His Gly Leu Thr Pro Gln Gln Val Val Ala Ile Ala Ser Asn Gly 
        675                 680                 685             


Gly Gly Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro 
    690                 695                 700                 


Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala 
705                 710                 715                 720 


Cys Leu Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Gly 
                725                 730                 735     


Asp Pro Ile Ser Arg Ser Gln Leu Val Lys Ser Glu Leu Glu Glu Lys 
            740                 745                 750         


Lys Ser Glu Leu Arg His Lys Leu Lys Tyr Val Pro His Glu Tyr Ile 
        755                 760                 765             


Glu Leu Ile Glu Ile Ala Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu 
    770                 775                 780                 


Met Lys Val Met Glu Phe Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys 
785                 790                 795                 800 


His Leu Gly Gly Ser Arg Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly 
                805                 810                 815     


Ser Pro Ile Asp Tyr Gly Val Ile Val Asp Thr Lys Ala Tyr Ser Gly 
            820                 825                 830         


Gly Tyr Asn Leu Pro Ile Gly Gln Ala Asp Glu Met Gln Arg Tyr Val 
        835                 840                 845             


Glu Glu Asn Gln Thr Arg Asn Lys His Ile Asn Pro Asn Glu Trp Trp 
    850                 855                 860                 


Lys Val Tyr Pro Ser Ser Val Thr Glu Phe Lys Phe Leu Phe Val Ser 
865                 870                 875                 880 


Gly His Phe Lys Gly Asn Tyr Lys Ala Gln Leu Thr Arg Leu Asn His 
                885                 890                 895     


Ile Thr Asn Cys Asn Gly Ala Val Leu Ser Val Glu Glu Leu Leu Ile 
            900                 905                 910         


Gly Gly Glu Met Ile Lys Ala Gly Thr Leu Thr Leu Glu Glu Val Arg 
        915                 920                 925             


Arg Lys Phe Asn Asn Gly Glu Ile Asn Phe Ala Ala Asp 
    930                 935                 940     


<210>  9

<211>  49
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN target DCK

<400>  9
tggttcctga acctgttgcc agatggtgca atgttcaaag tactcaaga                 49


<210>  10

<211>  49
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN target TRAC

<400>  10
ttgtcccaca gatatccaga accctgaccc tgccgtgtac cagctgaga                 49


<210>  11

<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN target CD25

<400>  11
tacaggagga agagtagaag aacaatctag aaaaccaaaa gaaca                     45


<210>  12

<211>  49
<212>  DNA
<213>  artificial sequence

<220>
<223>  TALEN target PD1

<400>  12
tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagaga                 49


<210>  13

<211>  3017
<212>  DNA
<213>  artificial sequence

<220>
<223>  Inserted matrice TRAC locus_CubiCAR CD22

<400>  13
atgagatcat gtcctaaccc tgatcctctt gtcccacaga tatccagaac cctgaccctg     60
ttgctgggcc tttttcccat gcctgccttt actctgccag agttatattg ctggggtttt    120
gaagaagatc ctattaaata aaagaataag cagtattatt aagtagccct gcatttcagg    180
tttccttgag tggcaggcca ggcctggccg tgaacgttca ctgaaatcat ggcctcttgg    240
ccaagattga tagcttgtgc ctgtccctga gtcccagtcc atcacgagca gctggtttct    300
aagatgctat ttcccgtata aagcatgaga ccgtgacttg ccagccccac agagccccgc    360
ccttgtccat cactggcatc tggactccag cctgggttgg ggcaaagagg gaaatgagat    420
catgtcctaa ccctgatcct cttgtcccac agatatccag tacccctacg acgtgcccga    480
ctacgcctcc ggtgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc    540
gggccccgga tccgctctgc ccgtcaccgc tctgctgctg ccactggcac tgctgctgca    600
cgctgctagg cccggagggg gaggcagctg cccctacagc aaccccagcc tgtgcagcgg    660
aggcggcggc agcggcggag ggggtagcca ggtgcagctg cagcagagcg gccctggcct    720
ggtgaagcca agccagacac tgtccctgac ctgcgccatc agcggcgatt ccgtgagctc    780
caactccgcc gcctggaatt ggatcaggca gtccccttct cggggcctgg agtggctggg    840
aaggacatac tatcggtcta agtggtacaa cgattatgcc gtgtctgtga agagcagaat    900
cacaatcaac cctgacacct ccaagaatca gttctctctg cagctgaata gcgtgacacc    960
agaggacacc gccgtgtact attgcgccag ggaggtgacc ggcgacctgg aggatgcctt   1020
tgacatctgg ggccagggca caatggtgac cgtgagctcc ggaggcggcg gatctggcgg   1080
aggaggaagt gggggcggcg ggagtgatat ccagatgaca cagtccccat cctctctgag   1140
cgcctccgtg ggcgacagag tgacaatcac ctgtagggcc tcccagacca tctggtctta   1200
cctgaactgg tatcagcaga ggcccggcaa ggcccctaat ctgctgatct acgcagcaag   1260
ctccctgcag agcggagtgc catccagatt ctctggcagg ggctccggca cagacttcac   1320
cctgaccatc tctagcctgc aggccgagga cttcgccacc tactattgcc agcagtctta   1380
tagcatcccc cagacatttg gccagggcac caagctggag atcaagtcgg atcccggaag   1440
cggaggggga ggcagctgcc cctacagcaa ccccagcctg tgcagcggag gcggcggcag   1500
cgagctgccc acccagggca ccttctccaa cgtgtccacc aacgtgagcc cagccaagcc   1560
caccaccacc gcctgtcctt attccaatcc ttccctgtgt gctcccacca caacccccgc   1620
tccaaggccc cctacccccg caccaactat tgcctcccag ccactctcac tgcggcctga   1680
ggcctgtcgg cccgctgctg gaggcgcagt gcatacaagg ggcctcgatt tcgcctgcga   1740
tatttacatc tgggcacccc tcgccggcac ctgcggggtg cttctcctct ccctggtgat   1800
taccctgtat tgcagacggg gccggaagaa gctcctctac atttttaagc agcctttcat   1860
gcggccagtg cagacaaccc aagaggagga tgggtgttcc tgcagattcc ctgaggaaga   1920
ggaaggcggg tgcgagctga gagtgaagtt ctccaggagc gcagatgccc ccgcctatca   1980
acagggccag aaccagctct acaacgagct taacctcggg aggcgcgaag aatacgacgt   2040
gttggataag agaagggggc gggaccccga gatgggagga aagccccgga ggaagaaccc   2100
tcaggagggc ctgtacaacg agctgcagaa ggataagatg gccgaggcct actcagagat   2160
cgggatgaag ggggagcggc gccgcgggaa ggggcacgat gggctctacc aggggctgag   2220
cacagccaca aaggacacat acgacgcctt gcacatgcag gcccttccac cccgggaata   2280
gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc   2340
atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt   2400
cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct   2460
ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc   2520
tggggatgcg gtgggctcta tgactagtgg cgaattcccg tgtaccagct gagagactct   2580
aaatccagtg acaagtctgt ctgcctattc accgattttg attctcaaac aaatgtgtca   2640
caaagtaagg attctgatgt gtatatcaca gacaaaactg tgctagacat gaggtctatg   2700
gacttcaaga gcaacagtgc tgtggcctgg agcaacaaat ctgactttgc atgtgcaaac   2760
gccttcaaca acagcattat tccagaagac accttcttcc ccagcccagg taagggcagc   2820
tttggtgcct tcgcaggctg tttccttgct tcaggaatgg ccaggttctg cccagagctc   2880
tggtcaatga tgtctaaaac tcctctgatt ggtggtctcg gccttatcca ttgccaccaa   2940
aaccctcttt ttactaagaa acagtgagcc ttgttctggc agtccagaga atgacacggg   3000
aaaaaagcag atgaaga                                                  3017


<210>  14

<211>  2808
<212>  DNA
<213>  artificial sequence

<220>
<223>  Inserted matrice CD25 locus_IL15_2A_sIL15Ra

<400>  14
agtgctggct agaaaccaag tgctttactg catgcacatc atttagcaca gttagttgct     60
gtttattatt cctgttccac agctattgtc tgccatataa aaacttaggc caggcacagt    120
ggctcacacc tgtaatccca gcactttgga aggccgaggc aggcagatca caaggtcagg    180
agttcgagac cagcctggcc aacatagcaa aaccccatct ctactaaaaa tacaaaaatt    240
agccaggcat ggtggcgtgt gcactggttt agagtgagga ccacattttt ttggtgccgt    300
gttacacata tgaccgtgac tttgttacac cactacagga ggaagagtag aagaacaatc    360
ggttctggcg tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtggag    420
tccaacccag ggcccggtac cgggtccgcc accatggact ggacctggat tctgttcctc    480
gtggctgctg ctacaagagt gcacagcggc attcatgtct tcattttggg ctgtttcagt    540
gcagggcttc ctaaaacaga agccaactgg gtgaatgtaa taagtgattt gaaaaaaatt    600
gaagatctta ttcaatctat gcatattgat gctactttat atacggaaag tgatgttcac    660
cccagttgca aagtaacagc aatgaagtgc tttctcttgg agttacaagt tatttcactt    720
gagtccggag atgcaagtat tcatgataca gtagaaaatc tgatcatcct agcaaacaac    780
agtttgtctt ctaatgggaa tgtaacagaa tctggatgca aagaatgtga ggaactggag    840
gaaaaaaata ttaaagaatt tttgcagagt tttgtacata ttgtccaaat gttcatcaac    900
acttctggaa gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag    960
aaccctggac ctgggaccgg ctctgcaacc atggattgga cgtggatcct gtttctcgtg   1020
gcagctgcca caagagttca cagtatcacg tgccctcccc ccatgtccgt ggaacacgca   1080
gacatctggg tcaagagcta cagcttgtac tccagggagc ggtacatttg taactctggt   1140
ttcaagcgta aagccggcac gtccagcctg acggagtgcg tgttgaacaa ggccacgaat   1200
gtcgcccact ggacaacccc cagtctcaaa tgcattagag accctgccct ggttcaccaa   1260
aggccagcgc caccctccac agtaacgacg gcaggggtga ccccacagcc agagagcctc   1320
tccccttctg gaaaagagcc cgcagcttca tctcccagct caaacaacac agcggccaca   1380
acagcagcta ttgtcccggg ctcccagctg atgccttcaa aatcaccttc cacaggaacc   1440
acagagataa gcagtcatga gtcctcccac ggcaccccct ctcagacaac agccaagaac   1500
tgggaactca cagcatccgc ctcccaccag ccgccaggtg tgtatccaca gggccacagc   1560
gacaccactg agggcagagg cagcctgctg acctgcggcg acgtcgagga gaaccccggg   1620
cccatggggg caggtgccac cggccgcgcc atggacgggc cgcgcctgct gctgttgctg   1680
cttctggggg tgtcccttgg aggtgccaag gaggcatgcc ccacaggcct gtacacacac   1740
agcggtgagt gctgcaaagc ctgcaacctg ggcgagggtg tggcccagcc ttgtggagcc   1800
aaccagaccg tgtgtgagcc ctgcctggac agcgtgacgt tctccgacgt ggtgagcgcg   1860
accgagccgt gcaagccgtg caccgagtgc gtggggctcc agagcatgtc ggcgccgtgc   1920
gtggaggccg atgacgccgt gtgccgctgc gcctacggct actaccagga tgagacgact   1980
gggcgctgcg aggcgtgccg cgtgtgcgag gcgggctcgg gcctcgtgtt ctcctgccag   2040
gacaagcaga acaccgtgtg cgaggagtgc cccgacggca cgtattccga cgaggccaac   2100
cacgtggacc cgtgcctgcc ctgcaccgtg tgcgaggaca ccgagcgcca gctccgcgag   2160
tgcacacgct gggccgacgc cgagtgcgag gagatccctg gccgttggat tacacggtcc   2220
acacccccag agggctcgga cagcacagcc cccagcaccc aggagcctga ggcacctcca   2280
gaacaagacc tcatagccag cacggtggca ggtgtggtga ccacagtgat gggcagctcc   2340
cagcccgtgg tgacccgagg caccaccgac aacctcatcc ctgtctattg ctccatcctg   2400
gctgctgtgg ttgtgggtct tgtggcctac atagccttca agaggtgaaa aaccaaaaga   2460
acaagaattt cttggtaaga agccgggaac agacaacaga agtcatgaag cccaagtgaa   2520
atcaaaggtg ctaaatggtc gcccaggaga catccgttgt gcttgcctgc gttttggaag   2580
ctctgaagtc acatcacagg acacggggca gtggcaacct tgtctctatg ccagctcagt   2640
cccatcagag agcgagcgct acccacttct aaatagcaat ttcgccgttg aagaggaagg   2700
gcaaaaccac tagaactctc catcttattt tcatgtatat gtgttcatta aagcatgaat   2760
ggtatggaac tctctccacc ctatatgtag tataaagaaa agtaggtt                2808


<210>  15

<211>  3084
<212>  DNA
<213>  artificial sequence

<220>
<223>  Inserted matrice PD1 locus_IL15_2A_sIL15Ra

<400>  15
ggtggccggg gaggctttgt ggggccaccc agccccttcc tcacctctct ccatctctca     60
gactccccag acaggccctg gaaccccccc accttctccc cagccctgct cgtggtgacc    120
gaaggggaca acgccacctt cacctgcagc ttctccaaca catcggagag cttcgtgcta    180
aactggtacc gcatgagccc cagcaaccag acggacaagc tggccgcctt ccccgaggac    240
cgcagccagc ccggccagga ctgccgcttc cgtgtcacac aactgcccaa cgggcgtgac    300
ttccacatga gcgtggtcag ggcccggcgc aatgacagcg gcacctacct ctgtggggcc    360
ggttctggcg tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtggag    420
tccaacccag ggcccggtac cgggtccgcc accatggact ggacctggat tctgttcctc    480
gtggctgctg ctacaagagt gcacagcggc attcatgtct tcattttggg ctgtttcagt    540
gcagggcttc ctaaaacaga agccaactgg gtgaatgtaa taagtgattt gaaaaaaatt    600
gaagatctta ttcaatctat gcatattgat gctactttat atacggaaag tgatgttcac    660
cccagttgca aagtaacagc aatgaagtgc tttctcttgg agttacaagt tatttcactt    720
gagtccggag atgcaagtat tcatgataca gtagaaaatc tgatcatcct agcaaacaac    780
agtttgtctt ctaatgggaa tgtaacagaa tctggatgca aagaatgtga ggaactggag    840
gaaaaaaata ttaaagaatt tttgcagagt tttgtacata ttgtccaaat gttcatcaac    900
acttctggaa gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag    960
aaccctggac ctgggaccgg ctctgcaacc atggattgga cgtggatcct gtttctcgtg   1020
gcagctgcca caagagttca cagtatcacg tgccctcccc ccatgtccgt ggaacacgca   1080
gacatctggg tcaagagcta cagcttgtac tccagggagc ggtacatttg taactctggt   1140
ttcaagcgta aagccggcac gtccagcctg acggagtgcg tgttgaacaa ggccacgaat   1200
gtcgcccact ggacaacccc cagtctcaaa tgcattagag accctgccct ggttcaccaa   1260
aggccagcgc caccctccac agtaacgacg gcaggggtga ccccacagcc agagagcctc   1320
tccccttctg gaaaagagcc cgcagcttca tctcccagct caaacaacac agcggccaca   1380
acagcagcta ttgtcccggg ctcccagctg atgccttcaa aatcaccttc cacaggaacc   1440
acagagataa gcagtcatga gtcctcccac ggcaccccct ctcagacaac agccaagaac   1500
tgggaactca cagcatccgc ctcccaccag ccgccaggtg tgtatccaca gggccacagc   1560
gacaccactg agggcagagg cagcctgctg acctgcggcg acgtcgagga gaaccccggg   1620
cccatggggg caggtgccac cggccgcgcc atggacgggc cgcgcctgct gctgttgctg   1680
cttctggggg tgtcccttgg aggtgccaag gaggcatgcc ccacaggcct gtacacacac   1740
agcggtgagt gctgcaaagc ctgcaacctg ggcgagggtg tggcccagcc ttgtggagcc   1800
aaccagaccg tgtgtgagcc ctgcctggac agcgtgacgt tctccgacgt ggtgagcgcg   1860
accgagccgt gcaagccgtg caccgagtgc gtggggctcc agagcatgtc ggcgccgtgc   1920
gtggaggccg atgacgccgt gtgccgctgc gcctacggct actaccagga tgagacgact   1980
gggcgctgcg aggcgtgccg cgtgtgcgag gcgggctcgg gcctcgtgtt ctcctgccag   2040
gacaagcaga acaccgtgtg cgaggagtgc cccgacggca cgtattccga cgaggccaac   2100
cacgtggacc cgtgcctgcc ctgcaccgtg tgcgaggaca ccgagcgcca gctccgcgag   2160
tgcacacgct gggccgacgc cgagtgcgag gagatccctg gccgttggat tacacggtcc   2220
acacccccag agggctcgga cagcacagcc cccagcaccc aggagcctga ggcacctcca   2280
gaacaagacc tcatagccag cacggtggca ggtgtggtga ccacagtgat gggcagctcc   2340
cagcccgtgg tgacccgagg caccaccgac aacctcatcc ctgtctattg ctccatcctg   2400
gctgctgtgg ttgtgggtct tgtggcctac atagccttca agaggtgatc tagagggccc   2460
gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc   2520
ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa   2580
aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg   2640
gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg   2700
ggctctatga ctagtggcga attcggcgca gatcaaagag agcctgcggg cagagctcag   2760
ggtgacaggt gcggcctcgg aggccccggg gcaggggtga gctgagccgg tcctggggtg   2820
ggtgtcccct cctgcacagg atcaggagct ccagggtcgt agggcaggga ccccccagct   2880
ccagtccagg gctctgtcct gcacctgggg aatggtgacc ggcatctctg tcctctagct   2940
ctggaagcac cccagcccct ctagtctgcc ctcacccctg accctgaccc tccaccctga   3000
ccccgtccta acccctgacc tttgtgccct tccagagaga agggcagaag tgcccacagc   3060
ccaccccagc ccctcaccca ggcc                                          3084



                                                2897






