                         SEQUENCE LISTING

<110>  Consejo Superior de Investigaciones Cientficas (CSIC)
 
<120>  Chimeric protein with high antimicrobial activity

<130>  PCT1641.1323

<150>  EP17382804
<151>  2017-11-28

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  92
<212>  PRT
<213>  Staphylococcus phage phiIPLA-RODI

<400>  1

Asp Ala Asp Gly Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp 
1               5                   10                  15      


Tyr Val Leu Trp Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala 
            20                  25                  30          


Lys Asp Gln Ile Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu 
        35                  40                  45              


Asn Lys Pro Ser Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr 
    50                  55                  60                  


Ser Gly Ser Tyr Gln Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly 
65                  70                  75                  80  


Gly Asn Thr Ser Thr Phe Thr Ile Leu Glu Gln Asn 
                85                  90          


<210>  2
<211>  165
<212>  PRT
<213>  Staphylococcus phage phiIPLA-RODI

<400>  2

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Lys Gly Thr Val Asp Ser Pro Tyr Arg Ile Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Gln Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala 
                165 


<210>  3
<211>  66
<212>  PRT
<213>  Staphylococcus virus IPLA88

<400>  3

Tyr Tyr Met Glu Glu Ser Ala Arg Phe Thr Asn Gly Asn Gln Pro Ile 
1               5                   10                  15      


Thr Val Arg Lys Val Gly Pro Phe Leu Ser Cys Pro Val Gly Tyr Gln 
            20                  25                  30          


Phe Gln Pro Gly Gly Tyr Cys Asp Tyr Thr Glu Val Met Leu Gln Asp 
        35                  40                  45              


Gly His Val Trp Val Gly Tyr Thr Trp Glu Gly Gln Arg Tyr Tyr Leu 
    50                  55                  60                  


Pro Ile 
65      


<210>  4
<211>  143
<212>  PRT
<213>  Staphylococcus virus IPLA88

<400>  4

Leu Leu Pro Glu Asp Lys Arg Leu Gln Leu Lys Asp Tyr Phe Ile Lys 
1               5                   10                  15      


Gln Ile Arg Ala Tyr Met Asp Gly Lys Ile Pro Val Ala Thr Val Ser 
            20                  25                  30          


Asn Asp Ser Ser Ala Ser Ser Asn Thr Val Lys Pro Val Ala Ser Ala 
        35                  40                  45              


Trp Lys Arg Asn Lys Tyr Gly Thr Tyr Tyr Met Glu Glu Ser Ala Arg 
    50                  55                  60                  


Phe Thr Asn Gly Asn Gln Pro Ile Thr Val Arg Lys Val Gly Pro Phe 
65                  70                  75                  80  


Leu Ser Cys Pro Val Gly Tyr Gln Phe Gln Pro Gly Gly Tyr Cys Asp 
                85                  90                  95      


Tyr Thr Glu Val Met Leu Gln Asp Gly His Val Trp Val Gly Tyr Thr 
            100                 105                 110         


Trp Glu Gly Gln Arg Tyr Tyr Leu Pro Ile Arg Thr Trp Asn Gly Ser 
        115                 120                 125             


Ala Pro Pro Asn Gln Ile Leu Gly Asp Leu Trp Gly Glu Ile Ser 
    130                 135                 140             


<210>  5
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric protein ClyRODI-H5

<400>  5

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Lys Gly Thr Val Asp Ser Pro Tyr Arg Ile Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Gln Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Leu Leu Pro Glu Asp Lys Arg Leu Gln Leu Lys 
                165                 170                 175     


Asp Tyr Phe Ile Lys Gln Ile Arg Ala Tyr Met Asp Gly Lys Ile Pro 
            180                 185                 190         


Val Ala Thr Val Ser Asn Asp Ser Ser Ala Ser Ser Asn Thr Val Lys 
        195                 200                 205             


Pro Val Ala Ser Ala Trp Lys Arg Asn Lys Tyr Gly Thr Tyr Tyr Met 
    210                 215                 220                 


Glu Glu Ser Ala Arg Phe Thr Asn Gly Asn Gln Pro Ile Thr Val Arg 
225                 230                 235                 240 


Lys Val Gly Pro Phe Leu Ser Cys Pro Val Gly Tyr Gln Phe Gln Pro 
                245                 250                 255     


Gly Gly Tyr Cys Asp Tyr Thr Glu Val Met Leu Gln Asp Gly His Val 
            260                 265                 270         


Trp Val Gly Tyr Thr Trp Glu Gly Gln Arg Tyr Tyr Leu Pro Ile Arg 
        275                 280                 285             


Thr Trp Asn Gly Ser Ala Pro Pro Asn Gln Ile Leu Gly Asp Leu Trp 
    290                 295                 300                 


Gly Glu Ile Ser 
305             


<210>  6
<211>  912
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  polynucleotide encoding the chimeric protein ClyRODI-H5

<400>  6
aaaacccagg cggagatcaa caagcgtctg gacgcgtacg cgaaaggtac cgtggatagc       60

ccgtaccgta ttaagaaagc gaccagctat gatccgagct tcggtgttat ggaagcgggc      120

gcgatcgacg cggatggtta ctatcacgcg cagtgccaag acctgattac cgattacgtg      180

ctgtggctga ccgacaacaa ggttcgtacc tggggcaacg cgaaagatca gatcaagcaa      240

agctatggta ccggcttcaa aattcacgag aacaagccga gcaccgtgcc gaagaaaggt      300

tggatcgcgg tttttaccag cggcagctac cagcaatggg gccacatcgg tattgtgtat      360

gatggtggca acaccagcac cttcaccatt ctggaacaga actggaacgg ttacgcgaac      420

aagaaaccga ccaaacgtgt tgacaactac tatggtctga cccactttat cgagattccg      480

gtgaaggcgc tgctgccgga agataaacgc ctgcagctga aggactattt tattaaacaa      540

atccgtgcgt acatggatgg caagattccg gtcgcgaccg tgtcaaacga cagttccgcc      600

tcatcgaata cggttaaacc ggtcgcctcg gcatggaaac gcaataagta tggtacctat      660

tacatggaag aaagcgcccg ttttaccaac ggcaatcagc cgatcacggt gcgcaaagtt      720

ggtccgtttc tgagctgccc ggtcggctat cagttccaac cgggtggcta ttgtgattac      780

accgaagtga tgctgcagga cggccatgtt tgggtcggtt acacgtggga aggccagcgt      840

tattacctgc cgattcgcac ctggaatggc tccgctccgc cgaatcaaat cctgggtgac      900

ctgtggggtg aa                                                          912


<210>  7
<211>  496
<212>  PRT
<213>  Staphylococcus phage philPLA-RODI

<400>  7

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Lys Gly Thr Val Asp Ser Pro Tyr Arg Ile Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Gln Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Gly Thr Thr Val Lys Lys Glu Thr Ala Lys Lys 
                165                 170                 175     


Ser Ala Ser Lys Thr Pro Ala Pro Lys Lys Lys Ala Thr Leu Lys Val 
            180                 185                 190         


Ser Lys Asn His Ile Asn Tyr Thr Met Asp Lys Arg Gly Lys Lys Pro 
        195                 200                 205             


Glu Gly Met Val Ile His Asn Asp Ala Gly Arg Ser Ser Gly Gln Gln 
    210                 215                 220                 


Tyr Glu Asn Ser Leu Ala Asn Ala Gly Tyr Ala Arg Tyr Ala Asn Gly 
225                 230                 235                 240 


Ile Ala His Tyr Tyr Gly Ser Glu Gly Tyr Val Trp Glu Ala Ile Asp 
                245                 250                 255     


Ala Lys Asn Gln Ile Ala Trp His Thr Gly Asp Gly Thr Gly Ala Asn 
            260                 265                 270         


Ser Gly Asn Phe Arg Phe Ala Gly Ile Glu Val Cys Gln Ser Met Ser 
        275                 280                 285             


Ala Ser Asp Ala Gln Phe Leu Lys Asn Glu Gln Ala Val Phe Gln Phe 
    290                 295                 300                 


Thr Ala Glu Lys Phe Lys Glu Trp Gly Leu Thr Pro Asn Arg Lys Thr 
305                 310                 315                 320 


Val Arg Leu His Met Glu Phe Val Pro Thr Ala Cys Pro His Arg Ser 
                325                 330                 335     


Met Val Leu His Thr Gly Phe Asn Pro Val Thr Gln Gly Arg Pro Ser 
            340                 345                 350         


Gln Ala Ile Met Asn Lys Leu Lys Asp Tyr Phe Ile Lys Gln Ile Lys 
        355                 360                 365             


Asn Tyr Met Asp Lys Gly Thr Ser Ser Ser Thr Ile Val Lys Asp Gly 
    370                 375                 380                 


Lys Thr Ser Ser Ala Ser Thr Pro Ala Thr Arg Pro Val Thr Gly Ser 
385                 390                 395                 400 


Trp Lys Lys Asn Gln Phe Gly Thr Trp Tyr Lys Pro Glu Ser Ala Thr 
                405                 410                 415     


Phe Val Asn Gly Asn Gln Pro Ile Ile Thr Arg Ile Gly Ser Pro Phe 
            420                 425                 430         


Leu Asn Ala Pro Ile Gly Gly Asn Leu Pro Ala Gly Ala Thr Ile Val 
        435                 440                 445             


Tyr Asp Glu Val Cys Ile Gln Ala Gly His Ile Trp Ile Gly Tyr Asn 
    450                 455                 460                 


Ala Tyr Asn Gly Asn Arg Val Tyr Cys Pro Val Arg Thr Cys Gln Gly 
465                 470                 475                 480 


Val Pro Pro Asn His Ile Pro Gly Val Ala Trp Gly Val Phe Lys Gly 
                485                 490                 495     


<210>  8
<211>  486
<212>  PRT
<213>  Staphylococcus virus IPLA88

<400>  8

Met Glu Val Ala Thr Met Gln Ala Lys Leu Thr Lys Lys Glu Phe Ile 
1               5                   10                  15      


Glu Trp Leu Lys Thr Ser Glu Gly Lys Gln Tyr Asn Ala Asp Gly Trp 
            20                  25                  30          


Tyr Gly Phe Gln Cys Phe Asp Tyr Ala Asn Ala Gly Trp Lys Ala Leu 
        35                  40                  45              


Phe Gly Leu Leu Leu Lys Gly Val Gly Ala Lys Asp Ile Pro Phe Ala 
    50                  55                  60                  


Asn Asn Phe Asp Gly Leu Ala Thr Val Tyr Gln Asn Thr Pro Asp Phe 
65                  70                  75                  80  


Leu Ala Gln Pro Gly Asp Met Val Val Phe Gly Ser Asn Tyr Gly Ala 
                85                  90                  95      


Gly Tyr Gly His Val Ala Trp Val Ile Glu Ala Thr Leu Asp Tyr Ile 
            100                 105                 110         


Ile Val Tyr Glu Gln Asn Trp Leu Gly Gly Gly Trp Thr Asp Gly Val 
        115                 120                 125             


Gln Gln Pro Gly Ser Gly Trp Glu Lys Val Thr Arg Arg Gln His Ala 
    130                 135                 140                 


Tyr Asp Phe Pro Met Trp Phe Ile Arg Pro Asn Phe Lys Ser Glu Thr 
145                 150                 155                 160 


Ala Pro Arg Ser Val Gln Ser Pro Thr Gln Ala Ser Lys Lys Glu Thr 
                165                 170                 175     


Ala Lys Pro Gln Pro Lys Ala Val Glu Leu Lys Ile Ile Lys Asp Val 
            180                 185                 190         


Val Lys Gly Tyr Asp Leu Pro Lys Arg Gly Ser Asn Pro Asn Phe Ile 
        195                 200                 205             


Val Ile His Asn Asp Ala Gly Ser Lys Gly Ala Thr Ala Glu Ala Tyr 
    210                 215                 220                 


Arg Asn Gly Leu Val Asn Ala Pro Leu Ser Arg Leu Glu Ala Gly Ile 
225                 230                 235                 240 


Ala His Ser Tyr Val Ser Gly Asn Thr Val Trp Gln Ala Leu Asp Glu 
                245                 250                 255     


Ser Gln Val Gly Trp His Thr Ala Asn Gln Ile Gly Asn Lys Tyr Gly 
            260                 265                 270         


Tyr Gly Ile Glu Val Cys Gln Ser Met Gly Ala Asp Asn Ala Thr Phe 
        275                 280                 285             


Leu Lys Asn Glu Gln Ala Thr Phe Gln Glu Cys Ala Arg Leu Leu Lys 
    290                 295                 300                 


Lys Trp Gly Leu Pro Ala Asn Arg Asn Thr Ile Arg Leu His Asn Glu 
305                 310                 315                 320 


Phe Thr Ser Thr Ser Cys Pro His Arg Ser Ser Val Leu His Thr Gly 
                325                 330                 335     


Phe Asp Pro Val Thr Arg Gly Leu Leu Pro Glu Asp Lys Arg Leu Gln 
            340                 345                 350         


Leu Lys Asp Tyr Phe Ile Lys Gln Ile Arg Ala Tyr Met Asp Gly Lys 
        355                 360                 365             


Ile Pro Val Ala Thr Val Ser Asn Asp Ser Ser Ala Ser Ser Asn Thr 
    370                 375                 380                 


Val Lys Pro Val Ala Ser Ala Trp Lys Arg Asn Lys Tyr Gly Thr Tyr 
385                 390                 395                 400 


Tyr Met Glu Glu Ser Ala Arg Phe Thr Asn Gly Asn Gln Pro Ile Thr 
                405                 410                 415     


Val Arg Lys Val Gly Pro Phe Leu Ser Cys Pro Val Gly Tyr Gln Phe 
            420                 425                 430         


Gln Pro Gly Gly Tyr Cys Asp Tyr Thr Glu Val Met Leu Gln Asp Gly 
        435                 440                 445             


His Val Trp Val Gly Tyr Thr Trp Glu Gly Gln Arg Tyr Tyr Leu Pro 
    450                 455                 460                 


Ile Arg Thr Trp Asn Gly Ser Ala Pro Pro Asn Gln Ile Leu Gly Asp 
465                 470                 475                 480 


Leu Trp Gly Glu Ile Ser 
                485     


