                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  Polypeptide

<130>  P113725PCT

<150>  GB 1719557.9
<151>  2017-11-24

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endodomain derived from human common gamma chain

<400>  1

Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu 
1               5                   10                  15      


Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys 
            20                  25                  30          


Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu 
        35                  40                  45              


Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly 
    50                  55                  60                  


Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr 
65                  70                  75                  80  


Thr Leu Lys Pro Glu Thr 
                85      


<210>  2
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endodomain derived from human IL-2Rbeta

<400>  2

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
225                 230                 235                 240 


Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu 
                245                 250                 255     


Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
            260                 265                 270         


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
        275                 280                 285     


<210>  3
<211>  195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endodomain derived from human IL-7Ralpha

<400>  3

Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro Asp His Lys 
1               5                   10                  15      


Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn Leu Asn Val 
            20                  25                  30          


Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His Arg Val Asp 
        35                  40                  45              


Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln Asp Thr Phe 
    50                  55                  60                  


Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln Arg Leu Gly Gly Asp Val 
65                  70                  75                  80  


Gln Ser Pro Asn Cys Pro Ser Glu Asp Val Val Ile Thr Pro Glu Ser 
                85                  90                  95      


Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu Ala Gly Asn Val Ser Ala 
            100                 105                 110         


Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg Ser Leu Asp Cys Arg Glu 
        115                 120                 125             


Ser Gly Lys Asn Gly Pro His Val Tyr Gln Asp Leu Leu Leu Ser Leu 
    130                 135                 140                 


Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro Phe Ser Leu Gln Ser Gly 
145                 150                 155                 160 


Ile Leu Thr Leu Asn Pro Val Ala Gln Gly Gln Pro Ile Leu Thr Ser 
                165                 170                 175     


Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val Thr Met Ser Ser Phe Tyr 
            180                 185                 190         


Gln Asn Gln 
        195 


<210>  4
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, hinge-CH2CH3 of human IgG1

<400>  4

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp 
225                 230                 


<210>  5
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human CD8 stalk

<400>  5

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45      


<210>  6
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human IgG1 hinge

<400>  6

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Lys Asp Pro Lys 
            20  


<210>  7
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane derived from human common gamma chain

<400>  7

Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
1               5                   10                  15      


Val Tyr Phe Trp Leu 
            20      


<210>  8
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane derived from human IL-2Rbeta

<400>  8

Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly 
1               5                   10                  15      


Phe Ile Ile Leu Val Tyr Leu Leu Ile 
            20                  25  


<210>  9
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane derived from human IL-7Ralpha

<400>  9

Pro Ile Leu Leu Thr Ile Ser Ile Leu Ser Phe Phe Ser Val Ala Leu 
1               5                   10                  15      


Leu Val Ile Leu Ala Cys Val Leu Trp 
            20                  25  


<210>  10
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane derived from human IL-15Ralpha

<400>  10

Ala Ile Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val Ser 
1               5                   10                  15      


Leu Leu Ala Cys Tyr Leu 
            20          


<210>  11
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR, CDRL1

<400>  11

Lys Ala Ser Gln Asp Val Thr Thr Ala Val Ala 
1               5                   10      


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR, CDRL2

<400>  12

Trp Ala Ser Thr Arg His Thr 
1               5           


<210>  13
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR, CDRL3

<400>  13

Gln Gln His Ile Thr Pro Trp Thr 
1               5               


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR, CDRH1

<400>  14

Ser Tyr Gly Val His 
1               5   


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR, CDRH2

<400>  15

Val Ile Trp Ala Gly Gly Ser Thr Asx Tyr Asn Ser Ala Leu Met 
1               5                   10                  15  


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR, CDRH3

<400>  16

Glu Gly Ser Thr Val Asp Ser Met Asp Tyr 
1               5                   10  


<210>  17
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL domain

<400>  17

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Ser Ile Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Thr Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Leu Tyr Tyr Cys Gln Gln His Tyr Ile Thr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105     


<210>  18
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH domain

<400>  18

Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Gln Ser Gln Ser Leu 
1               5                   10                  15      


Ser Ile Thr Cys Thr Val Asp Gly Phe Ser Leu Thr Ser Tyr Gly Val 
            20                  25                  30          


His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Val 
        35                  40                  45              


Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met Ser Arg 
    50                  55                  60                  


Leu Asn Ile Asn Arg Asp Asn Ser Lys Ser Gln Ile Phe Leu Lys Met 
65                  70                  75                  80  


Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Glu 
                85                  90                  95      


Gly Ser Thr Val Asp Ser Met Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr 
    


<210>  19
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from TCR beta chain

<400>  19

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Gly 
            20      


<210>  20
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from IgG1

<400>  20

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  21
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from CD8a

<400>  21

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys 
            20  


<210>  22
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  22

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  23
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal Sequence from Murine heavy chain

<400>  23

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  24
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RQR8 marker gene sequence

<400>  24

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly 
            20                  25                  30          


Gly Gly Gly Ser Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser 
        35                  40                  45              


Thr Asn Val Ser Pro Ala Lys Pro Thr Thr Thr Ala Cys Pro Tyr Ser 
    50                  55                  60                  


Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Pro Ala Pro Arg Pro 
65                  70                  75                  80  


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
                85                  90                  95      


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
            100                 105                 110         


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
        115                 120                 125             


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg 
    130                 135                 140                 


Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val Val Arg Ala 
145                 150                 155                 


<210>  25
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A peptide cleavage site sequence

<400>  25

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly 
    


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal sequence from mouse kappa chain

<400>  26

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  27
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-gammaC Light Chain variable region

<400>  27

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Ser Ile Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Thr Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Leu Tyr Tyr Cys Gln Gln His Tyr Ile Thr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  28
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker sequence

<400>  28

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


<210>  29
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-gammaC Heavy Chain variable region

<400>  29

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Gln Ser 
1               5                   10                  15      


Gln Ser Leu Ser Ile Thr Cys Thr Val Asp Gly Phe Ser Leu Thr Ser 
            20                  25                  30          


Tyr Gly Val His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu 
    50                  55                  60                  


Met Ser Arg Leu Asn Ile Asn Arg Asp Asn Ser Lys Ser Gln Ile Phe 
65                  70                  75                  80  


Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Gly Ser Thr Val Asp Ser Met Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA tag sequence

<400>  30

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  31
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-IL2receptor beta (CD122) specific variable light chain

<400>  31

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 
1               5                   10                  15      


Val Ile Ser Arg Gly Gln Trp Leu Thr Gln Ser Pro Val Ile Met Ser 
            20                  25                  30          


Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Ser Ala Ile Ser Ser 
        35                  40                  45              


Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Arg Leu 
    50                  55                  60                  


Leu Ile Tyr Asp Thr Ser Asn Leu Val Ser Gly Val Pro Val Arg Phe 
65                  70                  75                  80  


Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met 
                85                  90                  95      


Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Asn Thr Tyr 
            100                 105                 110         


Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125 


<210>  32
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-IL2receptor beta (CD122) specific variable heavy chain

<400>  32

Met Lys Leu Trp Leu Asn Trp Val Phe Leu Leu Thr Leu Leu His Gly 
1               5                   10                  15      


Ile Gln Cys Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Asp Phe Tyr Met Glu Trp Val Arg Gln Pro Pro Gly Lys Arg Leu 
    50                  55                  60                  


Glu Trp Ile Ala Ala Ser Arg Asn Lys Ala Asn Asp Tyr Thr Thr Glu 
65                  70                  75                  80  


Tyr Ser Ala Ser Val Lys Gly Arg Phe Ile Val Ser Arg Asp Thr Ser 
                85                  90                  95      


Gln Ser Ile Leu Tyr Leu Gln Met Asn Ala Leu Arg Ala Glu Asp Thr 
            100                 105                 110         


Ala Ile Tyr Tyr Cys Ala Arg Ser Tyr Tyr Arg Tyr Asp Gly Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
    130                 135                 140 


<210>  33
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid motif (Type I cytokine receptors)


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  33

Trp Ser Xaa Trp Ser 
1               5   


