                         序列表

<110>  邦泰生物工程（深圳）有限公司
       江西邦泰绿色生物合成生态产业园发展有限公司

<120>  一种酶改质甜菊糖的制备方法和制备用酶及应用

<160>  8

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1731
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  1
acccatagca cccgcggccg cgtgcgccgc gtgctggcgg gcggcctggc gaccagcacc        60
ctggcggcgg cggtgctgat tgcgggcgcg gcgccggcga ccgcgcagag cggcctgcag       120
gatggcccgg aaccgaccat tcatacccag caggcgtatg cgccggaaga tgattttacc       180
gcgaaatgga cccgcgcgga tgcgcgccag ctgcagcgca tgagcgatcc gaccgcgccg       240
agccgcgaaa acagcatgcc ggcgagcgtg accatgccga ccgtgccgca ggattttccg       300
gatatgagca acgaacaggt gtgggtgtgg gatacctggc cgctgaccga tgaagatgcg       360
aaccagtata gcgtgaacgg ctgggaaatt atttttagcc tggtggcgga tcgcaacctg       420
ggctttgatg atcgccatgt gtttgcgaaa attggctatt tttatcgccc ggcgggcgtg       480
ccggcggcgg aacgcccgga aaacggcggc tggacctatg gcggcctggt gtttaaagaa       540
ggcgtgaccg gccagatttt tgaagatcag agctttagcc atcagaccca gtggagcggc       600
agcgcgcgcg tgagcaaaaa cggcgaaatt aaactgtttt ttaccgatgt ggcgttttat       660
cgcaacagcg atggcaccaa cattaaaccg tatgatccgc gcattgcgct gagcgtgggc       720
aaagtgaaag cgaacaaaaa aggcgtgacc ctgaccggct ttaacaaagt gaccgatctg       780
ctgcaggcgg atggcaccta ttatcagacc ggcgcgcaga acgaattttt taactttcgc       840
gatccgttta cctttgaaga tccggcgcat ccgggcgaaa cctttatggt gtttgaaggc       900
aacagcgcga tgcagcgcga aaccgcgacc tgcaacgaag cggatctggg ctatcgccag       960
ggcgatccgt atgcggaaac cgtggatgat gtgaacgcga gcggcgcgac ctatcagatt      1020
ggcaacgtgg gcctggcgaa agcgaaaaac aaacagctga ccgaatggga atttctgccg      1080
ccgattctga gcgcgaactg cgtgaccgat cagaccgaac gcccgcagat ttattttaaa      1140
gatggcaaaa gctatctgtt taccattagc catcgcggca cctttgcggc gggcctggat      1200
ggcccggaag gcgtgtatgg ctttgtgggc gatggcattc gcagcgatta tcagccgctg      1260
aacggcggca gcggcctggc gctgggcaac ccgaccaacc tgaactttct gggcggccag      1320
ccgtttgcgc cggattttaa ccagcatccg ggccattttc aggcgtatag ccattatgtg      1380
atgccgggcg gcctggtgca gagctttatt gataccattg gcacccatga tgattttgtg      1440
cgcggcggca ccctggcgcc gaccgtgaaa atggatattg gcgtgggcgg cgatccgacc      1500
aaaaccgcgg tggattatag ctatggcagc gaaggcctgg gcggctgggc ggatattccg      1560
gcgaacaaac atctgtttac caacggcaaa tttggcgtgg cggtgagcga tgaagcggcg      1620
cagaaaattc gcaaaattct gggcagcaaa tttgatgatt atctggatgg caaaccggtg      1680
agcgcgaccg tgcgcgcgct gattgaaaaa ctgctggcgc agtatggcgg c               1731

<210>  2
<211>  1911
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  2
aaactgacca ccaccaccct ggcgctggcg accggcgcgg cggcggcgga agcgagctat        60
catctggata ccaccgcgcc gccgccgacc aacctgagca ccctgccgaa caacaccctg       120
tttcatgtgt ggcgcccgcg cgcgcatatt ctgccggcgg aaggccagat tggcgatccg       180
tgcgcgcatt ataccgatcc gagcaccggc ctgtttcatg tgggctttct gcatgatggc       240
gatggcattg cgggcgcgac caccgcgaac ctggcgacct ataccgatac cagcgataac       300
tttctgattc agccgggcgg caaaaacgat ccggtggcgg tgtttgatgg cgcggtgatt       360
ccggtgggcg tgaacaacac cccgaccctg ctgtatacca gcgtgagctt tctgccgatt       420
tggagcattc cgtatacccg cggcagcgaa acccagagcc tggcggtggc gcgcgatggc       480
ggccgccgct ttgataaact ggatcagggc ccggtgattg cggatcatcc gtttgcggtg       540
gatgtgaccg cgtttcgcga tccgtttgtg tttcgcagcg cgaaactgga tgtgctgctg       600
gatgaagaag tggcgcgcaa cgaaaccgcg gtgcagcagg cggtggatgg ctggaccgaa       660
aaaaacgcgc cgtggtatgt ggcggtgagc ggcggcgtgc atggcgtggg cccggcgcag       720
tttctgtatc gccagaacgg cggcaacgcg agcgaatttc agtattggga atatctgggc       780
gaatggtggc aggaagcgac caacagcagc tggggcgatg aaggcacctg ggcgggccgc       840
tggggcttta actttgaaac cggcaacgtg ctgtttctga ccgaagaagg ccatgatccg       900
cagaccggcg aagtgtttgt gaccctgggc accgaaggca gcggcctgcc gccgcaggtg       960
agcagcattc atgatatgct gtgggcggcg ggcgaagtgg gcgtgggcag cgaacaggaa      1020
aaagtggaat ttagcccgag catggcgggc tttctggatt ggggctttag cgcgtatgcg      1080
gcggcgggca aagtgctgcc ggcgagcagc gcggtgagca aaaccagcgg cgtggaagtg      1140
gatcgctatg tgagctttgt gtggctgacc ggcgatcagt atgaacaggc ggatggcttt      1200
ccgaccgcgc agcagggctg gggcagcctg ctgctgccgc gcgaactgaa aaccgtggaa      1260
aacgtggtgg ataacgaact ggtgcgcgaa gaaggcgtga gctgggtggt gggcgaaagc      1320
gataaccaga ccgcgcgcct gcgcaccctg ggcattacca ttgcgcgcga aaccaaagcg      1380
gcgctgctgg cgaacggcag cgtgaccgcg gaagaagatc gcaccctgca gaccgcggcg      1440
gtggtgccgt ttgcgcagag cccgagcagc aaattttttg tgctgaccgc gcagctggat      1500
gcgagcgcgc gcagcagccc gctgcagagc ggctttgaaa ttctggcgag cgaactggaa      1560
cgcaccgcga tttattatca gtttagcaac gaaagcctgg tggtggatcg cagccagacc      1620
agcgcggcgg cgccgaccaa cccggatagc tttaccgaaa gcggcaaact gcgcctgttt      1680
gatgtgattg aaaacggcca ggaacaggtg gaaaccctgg atctgaccgt ggtggtggat      1740
aacgcggtgg tggaagtgta tgcgaacggc cgctttgcgc tgagcacctg ggcgcgcagc      1800
tggtatgata acagcaccca gattcgcttt tttcataacg gcgaaggcga agtgcagttt      1860
cgcaacgtga gcgtgagcga aggcctgtat aacgcgtggc cggaacgcaa c               1911

<210>  3
<211>  577
<212>  PRT
<213>  嗜酸微杆菌属(Microbacterium saccharophilum)

<400>  3
Thr His Ser Thr Arg Gly Arg Val Arg Arg Val Leu Ala Gly Gly Leu 
1               5                   10                  15      
Ala Thr Ser Thr Leu Ala Ala Ala Val Leu Ile Ala Gly Ala Ala Pro 
            20                  25                  30          
Ala Thr Ala Gln Ser Gly Leu Gln Asp Gly Pro Glu Pro Thr Ile His 
        35                  40                  45              
Thr Gln Gln Ala Tyr Ala Pro Glu Asp Asp Phe Thr Ala Lys Trp Thr 
    50                  55                  60                  
Arg Ala Asp Ala Arg Gln Leu Gln Arg Met Ser Asp Pro Thr Ala Pro 
65                  70                  75                  80  
Ser Arg Glu Asn Ser Met Pro Ala Ser Val Thr Met Pro Thr Val Pro 
                85                  90                  95      
Gln Asp Phe Pro Asp Met Ser Asn Glu Gln Val Trp Val Trp Asp Thr 
            100                 105                 110         
Trp Pro Leu Thr Asp Glu Asp Ala Asn Gln Tyr Ser Val Asn Gly Trp 
        115                 120                 125             
Glu Ile Ile Phe Ser Leu Val Ala Asp Arg Asn Leu Gly Phe Asp Asp 
    130                 135                 140                 
Arg His Val Phe Ala Lys Ile Gly Tyr Phe Tyr Arg Pro Ala Gly Val 
145                 150                 155                 160 
Pro Ala Ala Glu Arg Pro Glu Asn Gly Gly Trp Thr Tyr Gly Gly Leu 
                165                 170                 175     
Val Phe Lys Glu Gly Val Thr Gly Gln Ile Phe Glu Asp Gln Ser Phe 
            180                 185                 190         
Ser His Gln Thr Gln Trp Ser Gly Ser Ala Arg Val Ser Lys Asn Gly 
        195                 200                 205             
Glu Ile Lys Leu Phe Phe Thr Asp Val Ala Phe Tyr Arg Asn Ser Asp 
    210                 215                 220                 
Gly Thr Asn Ile Lys Pro Tyr Asp Pro Arg Ile Ala Leu Ser Val Gly 
225                 230                 235                 240 
Lys Val Lys Ala Asn Lys Lys Gly Val Thr Leu Thr Gly Phe Asn Lys 
                245                 250                 255     
Val Thr Asp Leu Leu Gln Ala Asp Gly Thr Tyr Tyr Gln Thr Gly Ala 
            260                 265                 270         
Gln Asn Glu Phe Phe Asn Phe Arg Asp Pro Phe Thr Phe Glu Asp Pro 
        275                 280                 285             
Ala His Pro Gly Glu Thr Phe Met Val Phe Glu Gly Asn Ser Ala Met 
    290                 295                 300                 
Gln Arg Glu Thr Ala Thr Cys Asn Glu Ala Asp Leu Gly Tyr Arg Gln 
305                 310                 315                 320 
Gly Asp Pro Tyr Ala Glu Thr Val Asp Asp Val Asn Ala Ser Gly Ala 
                325                 330                 335     
Thr Tyr Gln Ile Gly Asn Val Gly Leu Ala Lys Ala Lys Asn Lys Gln 
            340                 345                 350         
Leu Thr Glu Trp Glu Phe Leu Pro Pro Ile Leu Ser Ala Asn Cys Val 
        355                 360                 365             
Thr Asp Gln Thr Glu Arg Pro Gln Ile Tyr Phe Lys Asp Gly Lys Ser 
    370                 375                 380                 
Tyr Leu Phe Thr Ile Ser His Arg Gly Thr Phe Ala Ala Gly Leu Asp 
385                 390                 395                 400 
Gly Pro Glu Gly Val Tyr Gly Phe Val Gly Asp Gly Ile Arg Ser Asp 
                405                 410                 415     
Tyr Gln Pro Leu Asn Gly Gly Ser Gly Leu Ala Leu Gly Asn Pro Thr 
            420                 425                 430         
Asn Leu Asn Phe Leu Gly Gly Gln Pro Phe Ala Pro Asp Phe Asn Gln 
        435                 440                 445             
His Pro Gly His Phe Gln Ala Tyr Ser His Tyr Val Met Pro Gly Gly 
    450                 455                 460                 
Leu Val Gln Ser Phe Ile Asp Thr Ile Gly Thr His Asp Asp Phe Val 
465                 470                 475                 480 
Arg Gly Gly Thr Leu Ala Pro Thr Val Lys Met Asp Ile Gly Val Gly 
                485                 490                 495     
Gly Asp Pro Thr Lys Thr Ala Val Asp Tyr Ser Tyr Gly Ser Glu Gly 
            500                 505                 510         
Leu Gly Gly Trp Ala Asp Ile Pro Ala Asn Lys His Leu Phe Thr Asn 
        515                 520                 525             
Gly Lys Phe Gly Val Ala Val Ser Asp Glu Ala Ala Gln Lys Ile Arg 
    530                 535                 540                 
Lys Ile Leu Gly Ser Lys Phe Asp Asp Tyr Leu Asp Gly Lys Pro Val 
545                 550                 555                 560 
Ser Ala Thr Val Arg Ala Leu Ile Glu Lys Leu Leu Ala Gln Tyr Gly 
                565                 570                 575     
Gly 
    

<210>  4
<211>  637
<212>  PRT
<213>  日本曲霉菌属(Aspergillus japonicus)

<400>  4
Lys Leu Thr Thr Thr Thr Leu Ala Leu Ala Thr Gly Ala Ala Ala Ala 
1               5                   10                  15      
Glu Ala Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu 
            20                  25                  30          
Ser Thr Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala 
        35                  40                  45              
His Ile Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr 
    50                  55                  60                  
Thr Asp Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly 
65                  70                  75                  80  
Asp Gly Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp 
                85                  90                  95      
Thr Ser Asp Asn Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
            100                 105                 110         
Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
        115                 120                 125             
Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile Trp Ser Ile Pro 
    130                 135                 140                 
Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp Gly 
145                 150                 155                 160 
Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ala Asp His 
                165                 170                 175     
Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe Arg 
            180                 185                 190         
Ser Ala Lys Leu Asp Val Leu Leu Asp Glu Glu Val Ala Arg Asn Glu 
        195                 200                 205             
Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys Asn Ala Pro 
    210                 215                 220                 
Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly Pro Ala Gln 
225                 230                 235                 240 
Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe Gln Tyr Trp 
                245                 250                 255     
Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser Ser Trp Gly 
            260                 265                 270         
Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe Glu Thr Gly 
        275                 280                 285             
Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln Thr Gly Glu 
    290                 295                 300                 
Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro Pro Gln Val 
305                 310                 315                 320 
Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly Glu Val Gly Val Gly 
                325                 330                 335     
Ser Glu Gln Glu Lys Val Glu Phe Ser Pro Ser Met Ala Gly Phe Leu 
            340                 345                 350         
Asp Trp Gly Phe Ser Ala Tyr Ala Ala Ala Gly Lys Val Leu Pro Ala 
        355                 360                 365             
Ser Ser Ala Val Ser Lys Thr Ser Gly Val Glu Val Asp Arg Tyr Val 
    370                 375                 380                 
Ser Phe Val Trp Leu Thr Gly Asp Gln Tyr Glu Gln Ala Asp Gly Phe 
385                 390                 395                 400 
Pro Thr Ala Gln Gln Gly Trp Gly Ser Leu Leu Leu Pro Arg Glu Leu 
                405                 410                 415     
Lys Thr Val Glu Asn Val Val Asp Asn Glu Leu Val Arg Glu Glu Gly 
            420                 425                 430         
Val Ser Trp Val Val Gly Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg 
        435                 440                 445             
Thr Leu Gly Ile Thr Ile Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala 
    450                 455                 460                 
Asn Gly Ser Val Thr Ala Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala 
465                 470                 475                 480 
Val Val Pro Phe Ala Gln Ser Pro Ser Ser Lys Phe Phe Val Leu Thr 
                485                 490                 495     
Ala Gln Leu Asp Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe 
            500                 505                 510         
Glu Ile Leu Ala Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe 
        515                 520                 525             
Ser Asn Glu Ser Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala 
    530                 535                 540                 
Pro Thr Asn Pro Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe 
545                 550                 555                 560 
Asp Val Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr 
                565                 570                 575     
Val Val Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe 
            580                 585                 590         
Ala Leu Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile 
        595                 600                 605             
Arg Phe Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser 
    610                 615                 620                 
Val Ser Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635         

<210>  5
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
cgcggatcca tgacccatag cacccgcgg                                          29

<210>  6
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  6
cccaagcttc ggcggtatga cgcggtcgt                                          29

<210>  7
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  7
cgcggatcca tgaaactgac caccaccacc                                         30

<210>  8
<211>  31
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  8
cccaagcttc aacgcaaggc cggtgcgcaa t                                       31

