                         SEQUENCE LISTING

<110>  Julius-Maximilians-Universitt Wrzburg
 
<120>  CD19CART cells eliminate myeloma cells that express very low 
       levels of CD19

<130>  209818

<150>  EP 17202583.5
<151>  2017-11-20

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  2529
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding the CD19 CAR

<400>  1
atgctgctgc tggtgaccag cctgctgctg tgcgagctgc cccaccccgc ctttctgctg       60

atccccgaca tccagatgac ccagaccacc tccagcctga gcgccagcct gggcgaccgg      120

gtgaccatca gctgccgggc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aagcccgacg gcaccgtcaa gctgctgatc taccacacca gccggctgca cagcggcgtg      240

cccagccggt ttagcggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gaacaggaag atatcgccac ctacttttgc cagcagggca acacactgcc ctacaccttt      360

ggcggcggaa caaagctgga aatcaccggc agcacctccg gcagcggcaa gcctggcagc      420

ggcgagggca gcaccaaggg cgaggtgaag ctgcaggaaa gcggccctgg cctggtggcc      480

cccagccaga gcctgagcgt gacctgcacc gtgagcggcg tgagcctgcc cgactacggc      540

gtgagctgga tccggcagcc ccccaggaag ggcctggaat ggctgggcgt gatctggggc      600

agcgagacca cctactacaa cagcgccctg aagagccggc tgaccatcat caaggacaac      660

agcaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgcgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

accagcgtga ccgtgagcag cgaatctaag tacggaccgc cctgcccccc ttgccctatg      840

ttctgggtgc tggtggtggt cggaggcgtg ctggcctgct acagcctgct ggtcaccgtg      900

gccttcatca tcttttgggt gaaacggggc agaaagaaac tcctgtatat attcaaacaa      960

ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca     1020

gaagaagaag aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct     1080

gcctaccagc agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag     1140

tacgacgtcc tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg     1200

aagaaccccc aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac     1260

agcgagatcg gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag     1320

ggcctgtcca ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca     1380

aggctcgagg gcggcggaga gggcagagga agtcttctaa catgcggtga cgtggaggag     1440

aatcccggcc ctaggatgct tctcctggtg acaagccttc tgctctgtga gttaccacac     1500

ccagcattcc tcctgatccc acgcaaagtg tgtaacggaa taggtattgg tgaatttaaa     1560

gactcactct ccataaatgc tacgaatatt aaacacttca aaaactgcac ctccatcagt     1620

ggcgatctcc acatcctgcc ggtggcattt aggggtgact ccttcacaca tactcctcct     1680

ctggatccac aggaactgga tattctgaaa accgtaaagg aaatcacagg gtttttgctg     1740

attcaggctt ggcctgaaaa caggacggac ctccatgcct ttgagaacct agaaatcata     1800

cgcggcagga ccaagcaaca tggtcagttt tctcttgcag tcgtcagcct gaacataaca     1860

tccttgggat tacgctccct caaggagata agtgatggag atgtgataat ttcaggaaac     1920

aaaaatttgt gctatgcaaa tacaataaac tggaaaaaac tgtttgggac ctccggtcag     1980

aaaaccaaaa ttataagcaa cagaggtgaa aacagctgca aggccacagg ccaggtctgc     2040

catgccttgt gctcccccga gggctgctgg ggcccggagc ccagggactg cgtctcttgc     2100

cggaatgtca gccgaggcag ggaatgcgtg gacaagtgca accttctgga gggtgagcca     2160

agggagtttg tggagaactc tgagtgcata cagtgccacc cagagtgcct gcctcaggcc     2220

atgaacatca cctgcacagg acggggacca gacaactgta tccagtgtgc ccactacatt     2280

gacggccccc actgcgtcaa gacctgcccg gcaggagtca tgggagaaaa caacaccctg     2340

gtctggaagt acgcagacgc cggccatgtg tgccacctgt gccatccaaa ctgcacctac     2400

ggatgcactg ggccaggtct tgaaggctgt ccaacgaatg ggcctaagat cccgtccatc     2460

gccactggga tggtgggggc cctcctcttg ctgctggtgg tggccctggg gatcggcctc     2520

ttcatgtga                                                             2529


<210>  2
<211>  9382
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence representing the expression vector encoding 
       the CD19 CAR

<400>  2
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc       60

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      120

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      180

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      240

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      300

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      360

attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      420

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      480

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      540

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      600

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      660

gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta      720

ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt      780

aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt      840

ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg      900

gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa      960

agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt     1020

tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca     1080

gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc     1140

aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg     1200

ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac     1260

tcatttgcac cactgctgtg ccttggatct acaaatggca gtattcatcc acaattttaa     1320

aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac     1380

agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta     1440

ttacagggac agcagagatc cagtttgggg atcaattgca tgaagaatct gcttagggtt     1500

aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga     1560

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc     1620

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     1680

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     1740

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     1800

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     1860

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     1920

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     1980

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta     2040

cagatccaag ctgtgaccgg cgcctacggc tagcgccgcc accatgctgc tgctggtgac     2100

cagcctgctg ctgtgcgagc tgccccaccc cgcctttctg ctgatccccg acatccagat     2160

gacccagacc acctccagcc tgagcgccag cctgggcgac cgggtgacca tcagctgccg     2220

ggccagccag gacatcagca agtacctgaa ctggtatcag cagaagcccg acggcaccgt     2280

caagctgctg atctaccaca ccagccggct gcacagcggc gtgcccagcc ggtttagcgg     2340

cagcggctcc ggcaccgact acagcctgac catctccaac ctggaacagg aagatatcgc     2400

cacctacttt tgccagcagg gcaacacact gccctacacc tttggcggcg gaacaaagct     2460

ggaaatcacc ggcagcacct ccggcagcgg caagcctggc agcggcgagg gcagcaccaa     2520

gggcgaggtg aagctgcagg aaagcggccc tggcctggtg gcccccagcc agagcctgag     2580

cgtgacctgc accgtgagcg gcgtgagcct gcccgactac ggcgtgagct ggatccggca     2640

gccccccagg aagggcctgg aatggctggg cgtgatctgg ggcagcgaga ccacctacta     2700

caacagcgcc ctgaagagcc ggctgaccat catcaaggac aacagcaaga gccaggtgtt     2760

cctgaagatg aacagcctgc agaccgacga caccgccatc tactactgcg ccaagcacta     2820

ctactacggc ggcagctacg ccatggacta ctggggccag ggcaccagcg tgaccgtgag     2880

cagcgaatct aagtacggac cgccctgccc cccttgccct atgttctggg tgctggtggt     2940

ggtcggaggc gtgctggcct gctacagcct gctggtcacc gtggccttca tcatcttttg     3000

ggtgaaacgg ggcagaaaga aactcctgta tatattcaaa caaccattta tgagaccagt     3060

acaaactact caagaggaag atggctgtag ctgccgattt ccagaagaag aagaaggagg     3120

atgtgaactg cgggtgaagt tcagcagaag cgccgacgcc cctgcctacc agcagggcca     3180

gaatcagctg tacaacgagc tgaacctggg cagaagggaa gagtacgacg tcctggataa     3240

gcggagaggc cgggaccctg agatgggcgg caagcctcgg cggaagaacc cccaggaagg     3300

cctgtataac gaactgcaga aagacaagat ggccgaggcc tacagcgaga tcggcatgaa     3360

gggcgagcgg aggcggggca agggccacga cggcctgtat cagggcctgt ccaccgccac     3420

caaggatacc tacgacgccc tgcacatgca ggccctgccc ccaaggctcg agggcggcgg     3480

agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggat     3540

gcttctcctg gtgacaagcc ttctgctctg tgagttacca cacccagcat tcctcctgat     3600

cccacgcaaa gtgtgtaacg gaataggtat tggtgaattt aaagactcac tctccataaa     3660

tgctacgaat attaaacact tcaaaaactg cacctccatc agtggcgatc tccacatcct     3720

gccggtggca tttaggggtg actccttcac acatactcct cctctggatc cacaggaact     3780

ggatattctg aaaaccgtaa aggaaatcac agggtttttg ctgattcagg cttggcctga     3840

aaacaggacg gacctccatg cctttgagaa cctagaaatc atacgcggca ggaccaagca     3900

acatggtcag ttttctcttg cagtcgtcag cctgaacata acatccttgg gattacgctc     3960

cctcaaggag ataagtgatg gagatgtgat aatttcagga aacaaaaatt tgtgctatgc     4020

aaatacaata aactggaaaa aactgtttgg gacctccggt cagaaaacca aaattataag     4080

caacagaggt gaaaacagct gcaaggccac aggccaggtc tgccatgcct tgtgctcccc     4140

cgagggctgc tggggcccgg agcccaggga ctgcgtctct tgccggaatg tcagccgagg     4200

cagggaatgc gtggacaagt gcaaccttct ggagggtgag ccaagggagt ttgtggagaa     4260

ctctgagtgc atacagtgcc acccagagtg cctgcctcag gccatgaaca tcacctgcac     4320

aggacgggga ccagacaact gtatccagtg tgcccactac attgacggcc cccactgcgt     4380

caagacctgc ccggcaggag tcatgggaga aaacaacacc ctggtctgga agtacgcaga     4440

cgccggccat gtgtgccacc tgtgccatcc aaactgcacc tacggatgca ctgggccagg     4500

tcttgaaggc tgtccaacga atgggcctaa gatcccgtcc atcgccactg ggatggtggg     4560

ggccctcctc ttgctgctgg tggtggccct ggggatcggc ctcttcatgt gagcggccgc     4620

tctagacccg ggctgcagga attcgatatc aagcttatcg ataatcaacc tctggattac     4680

aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga     4740

tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc     4800

tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa     4860

cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc     4920

acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc     4980

atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc     5040

gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg     5100

attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct     5160

tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg     5220

agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgactagc cgtaccttta     5280

agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga     5340

ctggaagggc taattcactc ccaaagaaga caagatctgc tttttgcctg tactgggtct     5400

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     5460

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     5520

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagaatt     5580

cgatatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc aattcgccct     5640

atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc     5700

ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata     5760

gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga     5820

aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt     5880

ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat     5940

agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa     6000

cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta     6060

atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc     6120

ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc     6180

gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac     6240

acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt     6300

gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag     6360

acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca     6420

tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc     6480

agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat     6540

cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc     6600

aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg     6660

gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc     6720

agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat     6780

aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga     6840

gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc     6900

ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc     6960

aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt     7020

aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc     7080

tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc     7140

agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca     7200

ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca     7260

ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt     7320

ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta     7380

acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg     7440

agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc     7500

ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag     7560

cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa     7620

gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc     7680

cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc     7740

gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta     7800

caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag     7860

aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct     7920

tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga     7980

gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc     8040

ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt     8100

atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg     8160

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg     8220

caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc     8280

cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc     8340

accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata     8400

acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgaaa ttaaccctca     8460

ctaaagggaa caaaagctgg agctccaccg cggtggcggc ctcgaggtcg agatccggtc     8520

gaccagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt     8580

ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg     8640

cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt     8700

gcaaaaagct tcgacggtat cgattggctc atgtccaaca ttaccgccat gttgacattg     8760

attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat     8820

ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc     8880

ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca     8940

ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta     9000

tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta     9060

tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat     9120

cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga     9180

ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca     9240

aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg     9300

taggcgtgta cggaattcgg agtggcgagc cctcagatcc tgcatataag cagctgcttt     9360

ttgcctgtac tgggtctctc tg                                              9382


<210>  3
<211>  3180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding the CD20 CAR

<400>  3
atgttgctgc tggttacatc tctgctgctg tgcgagctgc cccatcctgc ctttctgctg       60

atccccgaca tcgtgctgac acagagccct gccatcctga gtgcttcccc aggcgagaaa      120

gtgaccatga cctgtagagc cagcagcagc gtgaactaca tggactggta tcagaagaag      180

cccggcagca gccccaagcc ttggatctac gccacaagca atctggccag cggagtgcct      240

gccagatttt ctggctctgg cagcggcaca agctacagcc tgacaatcag cagagtggaa      300

gccgaggatg ccgccaccta ctactgtcag cagtggtcct tcaatcctcc taccttcggc      360

ggaggcacca agctggaaat caagggctct acaagcggcg gaggatctgg cggtggaagt      420

ggcggaggcg gatcttctga agttcagctg caacagtctg gcgccgagct ggttaagcct      480

ggcgcctctg tgaagatgag ctgcaaggcc agcggctaca ccttcaccag ctacaacatg      540

cactgggtca agcagacccc tggacaggga ctcgagtgga tcggagccat ctatcccggc      600

aatggcgaca cctcctacaa ccagaagttc aagggcaaag ccacactgac cgccgacaag      660

agcagcagca cagcctacat gcagctgagc agcctgacca gcgaggacag cgccgattac      720

tactgcgcca gaagcaacta ctacggcagc tcctactggt tcttcgacgt gtggggagcc      780

ggcaccacag tgacagtgtc tagcgagtct aagtacggac cgccttgtcc tccttgtcca      840

gctcctcctg tggccggacc tagcgtgttc ctgttccccc caaagcccaa ggacaccctg      900

atgatcagcc ggacccccga agtgacctgc gtggtggtgg atgtgtccca ggaagatccc      960

gaggtgcagt tcaattggta cgtggacggc gtggaagtgc acaacgccaa gaccaagccc     1020

agagaggaac agttccagag cacctaccgg gtggtgtccg tgctgacagt gctgcaccag     1080

gactggctga acggcaaaga gtacaagtgc aaggtgtcca acaagggcct gcccagcagc     1140

atcgagaaaa ccatcagcaa ggccaagggc cagcctcgcg agccccaggt gtacacactg     1200

cctccaagcc aggaagagat gaccaagaac caggtgtccc tgacctgtct cgtgaagggc     1260

ttctacccca gcgacattgc cgtggaatgg gagagcaacg gccagcccga gaacaactac     1320

aagaccaccc cccctgtgct ggacagcgac ggctcattct tcctgtacag cagactgacc     1380

gtggacaaga gccggtggca ggaaggcaac gtgttcagct gcagcgtgat gcacgaggcc     1440

ctgcacaacc actacaccca gaagtccctg tctctgagcc tgggcaagat gttctgggtg     1500

ctggtggtcg tgggcggagt gctggcctgt tacagcctgc tcgtgaccgt ggccttcatc     1560

atcttttggg tcaagcgggg cagaaagaag ctgctgtata tcttcaagca gcccttcatg     1620

cggcccgtgc agaccacaca ggaagaggac ggctgctcct gccggttccc cgaggaagaa     1680

gaaggcggct gcgagctgag agtgaagttc agcagaagcg ccgacgcccc tgcctatcag     1740

cagggccaga accagctgta caacgagctg aacctgggca gacgggaaga gtacgacgtg     1800

ctggataagc ggagaggccg ggaccctgag atgggcggca agcctagaag aaagaacccc     1860

caggaaggcc tgtataacga actgcagaaa gacaagatgg ccgaggccta cagcgagatc     1920

ggaatgaagg gcgagcggag aagaggcaag ggccacgatg gcctgtacca gggactgagc     1980

accgccacca aggataccta tgacgcactg cacatgcagg ccctgccccc cagactcgag     2040

ggcggaggcg aaggcagagg atctctgctg acatgcggcg acgtggaaga gaaccctggc     2100

cccagaatgc tgctgctcgt gacaagcctg ctgctgtgcg agctgcccca ccctgccttt     2160

ctgctgatcc cccggaaagt gtgcaacggc atcggcatcg gagagttcaa ggacagcctg     2220

tccatcaacg ccaccaacat caagcacttc aagaattgca ccagcatcag cggcgacctg     2280

cacatcctgc cagtggcctt tagaggcgac agcttcaccc acaccccccc actggatcca     2340

caggaactgg atattctgaa aaccgtaaag gaaatcacag ggtttttgct gattcaggct     2400

tggcctgaaa acaggacgga cctccatgcc tttgagaacc tagaaatcat acgcggcagg     2460

accaagcaac atggtcagtt ttctcttgca gtcgtcagcc tgaacataac atccttggga     2520

ttacgctccc tcaaggagat aagtgatgga gatgtgataa tttcaggaaa caaaaatttg     2580

tgctatgcaa atacaataaa ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa     2640

attataagca acagaggtga aaacagctgc aaggccacag gccaggtctg ccatgccttg     2700

tgctcccccg agggctgctg gggcccggag cccagggact gcgtctcttg ccggaatgtc     2760

agccgaggca gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc aagggagttt     2820

gtggagaact ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc catgaacatc     2880

acctgcacag gacggggacc agacaactgt atccagtgtg cccactacat tgacggcccc     2940

cactgcgtca agacctgccc ggcaggagtc atgggagaaa acaacaccct ggtctggaag     3000

tacgcagacg ccggccatgt gtgccacctg tgccatccaa actgcaccta cggatgcact     3060

gggccaggtc ttgaaggctg tccaacgaat gggcctaaga tcccgtccat cgccactggg     3120

atggtggggg ccctcctctt gctgctggtg gtggccctgg ggatcggcct cttcatgtga     3180


<210>  4
<211>  10033
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence representing the expression vector encoding 
       the CD20 CAR

<400>  4
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc       60

tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg      120

taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg      180

aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt      240

gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg      300

actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga      360

attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa      420

acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga      480

aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc      540

agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat      600

agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa      660

gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta      720

ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt      780

aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt      840

ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg      900

gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa      960

agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt     1020

tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca     1080

gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc     1140

aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg     1200

ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac     1260

tcatttgcac cactgctgtg ccttggatct acaaatggca gtattcatcc acaattttaa     1320

aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac     1380

agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta     1440

ttacagggac agcagagatc cagtttgggg atcaattgca tgaagaatct gcttagggtt     1500

aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga     1560

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc     1620

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     1680

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     1740

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     1800

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     1860

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     1920

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     1980

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta     2040

cagatccaag ctgtgaccgg cgcctacggc tagcgccgcc accatgttgc tgctggttac     2100

atctctgctg ctgtgcgagc tgccccatcc tgcctttctg ctgatccccg acatcgtgct     2160

gacacagagc cctgccatcc tgagtgcttc cccaggcgag aaagtgacca tgacctgtag     2220

agccagcagc agcgtgaact acatggactg gtatcagaag aagcccggca gcagccccaa     2280

gccttggatc tacgccacaa gcaatctggc cagcggagtg cctgccagat tttctggctc     2340

tggcagcggc acaagctaca gcctgacaat cagcagagtg gaagccgagg atgccgccac     2400

ctactactgt cagcagtggt ccttcaatcc tcctaccttc ggcggaggca ccaagctgga     2460

aatcaagggc tctacaagcg gcggaggatc tggcggtgga agtggcggag gcggatcttc     2520

tgaagttcag ctgcaacagt ctggcgccga gctggttaag cctggcgcct ctgtgaagat     2580

gagctgcaag gccagcggct acaccttcac cagctacaac atgcactggg tcaagcagac     2640

ccctggacag ggactcgagt ggatcggagc catctatccc ggcaatggcg acacctccta     2700

caaccagaag ttcaagggca aagccacact gaccgccgac aagagcagca gcacagccta     2760

catgcagctg agcagcctga ccagcgagga cagcgccgat tactactgcg ccagaagcaa     2820

ctactacggc agctcctact ggttcttcga cgtgtgggga gccggcacca cagtgacagt     2880

gtctagcgag tctaagtacg gaccgccttg tcctccttgt ccagctcctc ctgtggccgg     2940

acctagcgtg ttcctgttcc ccccaaagcc caaggacacc ctgatgatca gccggacccc     3000

cgaagtgacc tgcgtggtgg tggatgtgtc ccaggaagat cccgaggtgc agttcaattg     3060

gtacgtggac ggcgtggaag tgcacaacgc caagaccaag cccagagagg aacagttcca     3120

gagcacctac cgggtggtgt ccgtgctgac agtgctgcac caggactggc tgaacggcaa     3180

agagtacaag tgcaaggtgt ccaacaaggg cctgcccagc agcatcgaga aaaccatcag     3240

caaggccaag ggccagcctc gcgagcccca ggtgtacaca ctgcctccaa gccaggaaga     3300

gatgaccaag aaccaggtgt ccctgacctg tctcgtgaag ggcttctacc ccagcgacat     3360

tgccgtggaa tgggagagca acggccagcc cgagaacaac tacaagacca ccccccctgt     3420

gctggacagc gacggctcat tcttcctgta cagcagactg accgtggaca agagccggtg     3480

gcaggaaggc aacgtgttca gctgcagcgt gatgcacgag gccctgcaca accactacac     3540

ccagaagtcc ctgtctctga gcctgggcaa gatgttctgg gtgctggtgg tcgtgggcgg     3600

agtgctggcc tgttacagcc tgctcgtgac cgtggccttc atcatctttt gggtcaagcg     3660

gggcagaaag aagctgctgt atatcttcaa gcagcccttc atgcggcccg tgcagaccac     3720

acaggaagag gacggctgct cctgccggtt ccccgaggaa gaagaaggcg gctgcgagct     3780

gagagtgaag ttcagcagaa gcgccgacgc ccctgcctat cagcagggcc agaaccagct     3840

gtacaacgag ctgaacctgg gcagacggga agagtacgac gtgctggata agcggagagg     3900

ccgggaccct gagatgggcg gcaagcctag aagaaagaac ccccaggaag gcctgtataa     3960

cgaactgcag aaagacaaga tggccgaggc ctacagcgag atcggaatga agggcgagcg     4020

gagaagaggc aagggccacg atggcctgta ccagggactg agcaccgcca ccaaggatac     4080

ctatgacgca ctgcacatgc aggccctgcc ccccagactc gagggcggag gcgaaggcag     4140

aggatctctg ctgacatgcg gcgacgtgga agagaaccct ggccccagaa tgctgctgct     4200

cgtgacaagc ctgctgctgt gcgagctgcc ccaccctgcc tttctgctga tcccccggaa     4260

agtgtgcaac ggcatcggca tcggagagtt caaggacagc ctgtccatca acgccaccaa     4320

catcaagcac ttcaagaatt gcaccagcat cagcggcgac ctgcacatcc tgccagtggc     4380

ctttagaggc gacagcttca cccacacccc cccactggat ccacaggaac tggatattct     4440

gaaaaccgta aaggaaatca cagggttttt gctgattcag gcttggcctg aaaacaggac     4500

ggacctccat gcctttgaga acctagaaat catacgcggc aggaccaagc aacatggtca     4560

gttttctctt gcagtcgtca gcctgaacat aacatccttg ggattacgct ccctcaagga     4620

gataagtgat ggagatgtga taatttcagg aaacaaaaat ttgtgctatg caaatacaat     4680

aaactggaaa aaactgtttg ggacctccgg tcagaaaacc aaaattataa gcaacagagg     4740

tgaaaacagc tgcaaggcca caggccaggt ctgccatgcc ttgtgctccc ccgagggctg     4800

ctggggcccg gagcccaggg actgcgtctc ttgccggaat gtcagccgag gcagggaatg     4860

cgtggacaag tgcaaccttc tggagggtga gccaagggag tttgtggaga actctgagtg     4920

catacagtgc cacccagagt gcctgcctca ggccatgaac atcacctgca caggacgggg     4980

accagacaac tgtatccagt gtgcccacta cattgacggc ccccactgcg tcaagacctg     5040

cccggcagga gtcatgggag aaaacaacac cctggtctgg aagtacgcag acgccggcca     5100

tgtgtgccac ctgtgccatc caaactgcac ctacggatgc actgggccag gtcttgaagg     5160

ctgtccaacg aatgggccta agatcccgtc catcgccact gggatggtgg gggccctcct     5220

cttgctgctg gtggtggccc tggggatcgg cctcttcatg tgagcggccg ctctagaccc     5280

gggctgcagg aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt     5340

gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct     5400

ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat     5460

aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg     5520

gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag     5580

ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc     5640

tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg     5700

tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc     5760

gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc     5820

ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc     5880

tccctttggg ccgcctcccc gcatcgatac cgtcgactag ccgtaccttt aagaccaatg     5940

acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg     6000

ctaattcact cccaaagaag acaagatctg ctttttgcct gtactgggtc tctctggtta     6060

gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa     6120

taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac     6180

tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagaat tcgatatcaa     6240

gcttatcgat accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt     6300

cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta     6360

cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg     6420

cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aaattgtaag     6480

cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca     6540

ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag     6600

tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg     6660

gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt     6720

tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag     6780

agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc     6840

gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc     6900

gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac     6960

ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc     7020

ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt     7080

cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct     7140

ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga     7200

tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag     7260

cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca     7320

actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga     7380

aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag     7440

tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc     7500

ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa     7560

tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt     7620

gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg     7680

gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt     7740

tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg     7800

gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat     7860

ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact     7920

gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa     7980

aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt     8040

ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt     8100

ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg     8160

tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca     8220

gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt     8280

agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga     8340

taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc     8400

gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact     8460

gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga     8520

caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg     8580

aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt     8640

tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt     8700

acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga     8760

ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac     8820

gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc     8880

tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa     8940

agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc     9000

tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca     9060

cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga     9120

acaaaagctg gagctccacc gcggtggcgg cctcgaggtc gagatccggt cgaccagcaa     9180

ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt     9240

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct     9300

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc     9360

ttcgacggta tcgattggct catgtccaac attaccgcca tgttgacatt gattattgac     9420

tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg     9480

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt     9540

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca     9600

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc     9660

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta     9720

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac     9780

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg     9840

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg     9900

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt     9960

acggaattcg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta    10020

ctgggtctct ctg                                                       10033


