                         SEQUENCE LISTING

<110>  Northwestern University
       Jewett, Michael Christopher
       Des Soye, Benjamin
       Davidson, Samuel Ryan
 
<120>  CELL-FREE PROTEIN SYNTHESIS PLATFORM DERIVED FROM CELLULAR 
       EXTRACTS OF VIBRIO NATRIEGENS

<130>  5369-00567

<150>  US 62/584,406
<151>  2017-11-10

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  117
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence encoding Cecropin A peptide

<400>  1
atgaagtgga aattgtttaa aaagatcgaa aaggtggggc aaaatatccg cgacgggatc       60

attaaggcag gtccggctgt ggcggtcgtt ggtcaggcaa cgcaaatcgc aaaataa         117


<210>  2
<211>  38
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of Cecropin A peptide

<400>  2

Met Lys Trp Lys Leu Phe Lys Lys Ile Glu Lys Val Gly Gln Asn Ile 
1               5                   10                  15      


Arg Asp Gly Ile Ile Lys Ala Gly Pro Ala Val Ala Val Val Gly Gln 
            20                  25                  30          


Ala Thr Gln Ile Ala Lys 
        35              


<210>  3
<211>  99
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence encoding Cecropin P1 peptide

<400>  3
atgagctggc tgagcaaaac cgcgaaaaaa ctggaaaaca gcgcgaaaaa acgcattagc       60

gaaggcattg cgattgcgat tcagggcggc ccgcgctaa                              99


<210>  4
<211>  32
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of Cecropin P1 peptide

<400>  4

Met Ser Trp Leu Ser Lys Thr Ala Lys Lys Leu Glu Asn Ser Ala Lys 
1               5                   10                  15      


Lys Arg Ile Ser Glu Gly Ile Ala Ile Ala Ile Gln Gly Gly Pro Arg 
            20                  25                  30          


<210>  5
<211>  138
<212>  DNA
<213>  Artificial

<220>
<223>  DNA sequence encoding Opistoporin I peptide

<400>  5
atgggcaaag tgtgggattg gattaaaagc accgcgaaaa aactgtggaa cagcgaaccg       60

gtgaaagaac tgaaaaacac cgcgctgaac gcggcgaaaa acctggtggc ggaaaaaatt      120

ggcgcgaccc cgagctaa                                                    138


<210>  6
<211>  45
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of Opistoporin I peptide

<400>  6

Met Gly Lys Val Trp Asp Trp Ile Lys Ser Thr Ala Lys Lys Leu Trp 
1               5                   10                  15      


Asn Ser Glu Pro Val Lys Glu Leu Lys Asn Thr Ala Leu Asn Ala Ala 
            20                  25                  30          


Lys Asn Leu Val Ala Glu Lys Ile Gly Ala Thr Pro Ser 
        35                  40                  45  


<210>  7
<211>  235
<212>  PRT
<213>  E. coli

<400>  7

Met Tyr Arg Tyr Leu Ser Ile Ala Ala Val Val Leu Ser Ala Ala Phe 
1               5                   10                  15      


Ser Gly Pro Ala Leu Ala Glu Gly Ile Asn Ser Phe Ser Gln Ala Lys 
            20                  25                  30          


Ala Ala Ala Val Lys Val His Ala Asp Ala Pro Gly Thr Phe Tyr Cys 
        35                  40                  45              


Gly Cys Lys Ile Asn Trp Gln Gly Lys Lys Gly Val Val Asp Leu Gln 
    50                  55                  60                  


Ser Cys Gly Tyr Gln Val Arg Lys Asn Glu Asn Arg Ala Ser Arg Val 
65                  70                  75                  80  


Glu Trp Glu His Val Val Pro Ala Trp Gln Phe Gly His Gln Arg Gln 
                85                  90                  95      


Cys Trp Gln Asp Gly Gly Arg Lys Asn Cys Ala Lys Asp Pro Val Tyr 
            100                 105                 110         


Arg Lys Met Glu Ser Asp Met His Asn Leu Gln Pro Ser Val Gly Glu 
        115                 120                 125             


Val Asn Gly Asp Arg Gly Asn Phe Met Tyr Ser Gln Trp Asn Gly Gly 
    130                 135                 140                 


Glu Gly Gln Tyr Gly Gln Cys Ala Met Lys Val Asp Phe Lys Glu Lys 
145                 150                 155                 160 


Ala Ala Glu Pro Pro Ala Arg Ala Arg Gly Ala Ile Ala Arg Thr Tyr 
                165                 170                 175     


Phe Tyr Met Arg Asp Gln Tyr Asn Leu Thr Leu Ser Arg Gln Gln Thr 
            180                 185                 190         


Gln Leu Phe Asn Ala Trp Asn Lys Met Tyr Pro Val Thr Asp Trp Glu 
        195                 200                 205             


Cys Glu Arg Asp Glu Arg Ile Ala Lys Val Gln Gly Asn His Asn Pro 
    210                 215                 220                 


Tyr Val Gln Arg Ala Cys Gln Ala Arg Lys Ser 
225                 230                 235 


<210>  8
<211>  784
<212>  PRT
<213>  E. coli

<400>  8

Met Asn Pro Glu Arg Ser Glu Arg Ile Glu Ile Pro Val Leu Pro Leu 
1               5                   10                  15      


Arg Asp Val Val Val Tyr Pro His Met Val Ile Pro Leu Phe Val Gly 
            20                  25                  30          


Arg Glu Lys Ser Ile Arg Cys Leu Glu Ala Ala Met Asp His Asp Lys 
        35                  40                  45              


Lys Ile Met Leu Val Ala Gln Lys Glu Ala Ser Thr Asp Glu Pro Gly 
    50                  55                  60                  


Val Asn Asp Leu Phe Thr Val Gly Thr Val Ala Ser Ile Leu Gln Met 
65                  70                  75                  80  


Leu Lys Leu Pro Asp Gly Thr Val Lys Val Leu Val Glu Gly Leu Gln 
                85                  90                  95      


Arg Ala Arg Ile Ser Ala Leu Ser Asp Asn Gly Glu His Phe Ser Ala 
            100                 105                 110         


Lys Ala Glu Tyr Leu Glu Ser Pro Thr Ile Asp Glu Arg Glu Gln Glu 
        115                 120                 125             


Val Leu Val Arg Thr Ala Ile Ser Gln Phe Glu Gly Tyr Ile Lys Leu 
    130                 135                 140                 


Asn Lys Lys Ile Pro Pro Glu Val Leu Thr Ser Leu Asn Ser Ile Asp 
145                 150                 155                 160 


Asp Pro Ala Arg Leu Ala Asp Thr Ile Ala Ala His Met Pro Leu Lys 
                165                 170                 175     


Leu Ala Asp Lys Gln Ser Val Leu Glu Met Ser Asp Val Asn Glu Arg 
            180                 185                 190         


Leu Glu Tyr Leu Met Ala Met Met Glu Ser Glu Ile Asp Leu Leu Gln 
        195                 200                 205             


Val Glu Lys Arg Ile Arg Asn Arg Val Lys Lys Gln Met Glu Lys Ser 
    210                 215                 220                 


Gln Arg Glu Tyr Tyr Leu Asn Glu Gln Met Lys Ala Ile Gln Lys Glu 
225                 230                 235                 240 


Leu Gly Glu Met Asp Asp Ala Pro Asp Glu Asn Glu Ala Leu Lys Arg 
                245                 250                 255     


Lys Ile Asp Ala Ala Lys Met Pro Lys Glu Ala Lys Glu Lys Ala Glu 
            260                 265                 270         


Ala Glu Leu Gln Lys Leu Lys Met Met Ser Pro Met Ser Ala Glu Ala 
        275                 280                 285             


Thr Val Val Arg Gly Tyr Ile Asp Trp Met Val Gln Val Pro Trp Asn 
    290                 295                 300                 


Ala Arg Ser Lys Val Lys Lys Asp Leu Arg Gln Ala Gln Glu Ile Leu 
305                 310                 315                 320 


Asp Thr Asp His Tyr Gly Leu Glu Arg Val Lys Asp Arg Ile Leu Glu 
                325                 330                 335     


Tyr Leu Ala Val Gln Ser Arg Val Asn Lys Ile Lys Gly Pro Ile Leu 
            340                 345                 350         


Cys Leu Val Gly Pro Pro Gly Val Gly Lys Thr Ser Leu Gly Gln Ser 
        355                 360                 365             


Ile Ala Lys Ala Thr Gly Arg Lys Tyr Val Arg Met Ala Leu Gly Gly 
    370                 375                 380                 


Val Arg Asp Glu Ala Glu Ile Arg Gly His Arg Arg Thr Tyr Ile Gly 
385                 390                 395                 400 


Ser Met Pro Gly Lys Leu Ile Gln Lys Met Ala Lys Val Gly Val Lys 
                405                 410                 415     


Asn Pro Leu Phe Leu Leu Asp Glu Ile Asp Lys Met Ser Ser Asp Met 
            420                 425                 430         


Arg Gly Asp Pro Ala Ser Ala Leu Leu Glu Val Leu Asp Pro Glu Gln 
        435                 440                 445             


Asn Val Ala Phe Ser Asp His Tyr Leu Glu Val Asp Tyr Asp Leu Ser 
    450                 455                 460                 


Asp Val Met Phe Val Ala Thr Ser Asn Ser Met Asn Ile Pro Ala Pro 
465                 470                 475                 480 


Leu Leu Asp Arg Met Glu Val Ile Arg Leu Ser Gly Tyr Thr Glu Asp 
                485                 490                 495     


Glu Lys Leu Asn Ile Ala Lys Arg His Leu Leu Pro Lys Gln Ile Glu 
            500                 505                 510         


Arg Asn Ala Leu Lys Lys Gly Glu Leu Thr Val Asp Asp Ser Ala Ile 
        515                 520                 525             


Ile Gly Ile Ile Arg Tyr Tyr Thr Arg Glu Ala Gly Val Arg Gly Leu 
    530                 535                 540                 


Glu Arg Glu Ile Ser Lys Leu Cys Arg Lys Ala Val Lys Gln Leu Leu 
545                 550                 555                 560 


Leu Asp Lys Ser Leu Lys His Ile Glu Ile Asn Gly Asp Asn Leu His 
                565                 570                 575     


Asp Tyr Leu Gly Val Gln Arg Phe Asp Tyr Gly Arg Ala Asp Asn Glu 
            580                 585                 590         


Asn Arg Val Gly Gln Val Thr Gly Leu Ala Trp Thr Glu Val Gly Gly 
        595                 600                 605             


Asp Leu Leu Thr Ile Glu Thr Ala Cys Val Pro Gly Lys Gly Lys Leu 
    610                 615                 620                 


Thr Tyr Thr Gly Ser Leu Gly Glu Val Met Gln Glu Ser Ile Gln Ala 
625                 630                 635                 640 


Ala Leu Thr Val Val Arg Ala Arg Ala Glu Lys Leu Gly Ile Asn Pro 
                645                 650                 655     


Asp Phe Tyr Glu Lys Arg Asp Ile His Val His Val Pro Glu Gly Ala 
            660                 665                 670         


Thr Pro Lys Asp Gly Pro Ser Ala Gly Ile Ala Met Cys Thr Ala Leu 
        675                 680                 685             


Val Ser Cys Leu Thr Gly Asn Pro Val Arg Ala Asp Val Ala Met Thr 
    690                 695                 700                 


Gly Glu Ile Thr Leu Arg Gly Gln Val Leu Pro Ile Gly Gly Leu Lys 
705                 710                 715                 720 


Glu Lys Leu Leu Ala Ala His Arg Gly Gly Ile Lys Thr Val Leu Ile 
                725                 730                 735     


Pro Phe Glu Asn Lys Arg Asp Leu Glu Glu Ile Pro Asp Asn Val Ile 
            740                 745                 750         


Ala Asp Leu Asp Ile His Pro Val Lys Arg Ile Glu Glu Val Leu Thr 
        755                 760                 765             


Leu Ala Leu Gln Asn Glu Pro Ser Gly Met Gln Val Val Thr Ala Lys 
    770                 775                 780                 


<210>  9
<211>  111
<212>  PRT
<213>  E. coli

<400>  9

Met Val Ser Arg Tyr Val Pro Asp Met Gly Asp Leu Ile Trp Val Asp 
1               5                   10                  15      


Phe Asp Pro Thr Lys Gly Ser Glu Gln Ala Gly His Arg Pro Ala Val 
            20                  25                  30          


Val Leu Ser Pro Phe Met Tyr Asn Asn Lys Thr Gly Met Cys Leu Cys 
        35                  40                  45              


Val Pro Cys Thr Thr Gln Ser Lys Gly Tyr Pro Phe Glu Val Val Leu 
    50                  55                  60                  


Ser Gly Gln Glu Arg Asp Gly Val Ala Leu Ala Asp Gln Val Lys Ser 
65                  70                  75                  80  


Ile Ala Trp Arg Ala Arg Gly Ala Thr Lys Lys Gly Thr Val Ala Pro 
                85                  90                  95      


Glu Glu Leu Gln Leu Ile Lys Ala Lys Ile Asn Val Leu Ile Gly 
            100                 105                 110     


<210>  10
<211>  317
<212>  PRT
<213>  E. coli

<400>  10

Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile 
1               5                   10                  15      


Ser Ser Phe Ala Ser Thr Glu Thr Leu Ser Phe Thr Pro Asp Asn Ile 
            20                  25                  30          


Asn Ala Asp Ile Ser Leu Gly Thr Leu Ser Gly Lys Thr Lys Glu Arg 
        35                  40                  45              


Val Tyr Leu Ala Glu Glu Gly Gly Arg Lys Val Ser Gln Leu Asp Trp 
    50                  55                  60                  


Lys Phe Asn Asn Ala Ala Ile Ile Lys Gly Ala Ile Asn Trp Asp Leu 
65                  70                  75                  80  


Met Pro Gln Ile Ser Ile Gly Ala Ala Gly Trp Thr Thr Leu Gly Ser 
                85                  90                  95      


Arg Gly Gly Asn Met Val Asp Gln Asp Trp Met Asp Ser Ser Asn Pro 
            100                 105                 110         


Gly Thr Trp Thr Asp Glu Ser Arg His Pro Asp Thr Gln Leu Asn Tyr 
        115                 120                 125             


Ala Asn Glu Phe Asp Leu Asn Ile Lys Gly Trp Leu Leu Asn Glu Pro 
    130                 135                 140                 


Asn Tyr Arg Leu Gly Leu Met Ala Gly Tyr Gln Glu Ser Arg Tyr Ser 
145                 150                 155                 160 


Phe Thr Ala Arg Gly Gly Ser Tyr Ile Tyr Ser Ser Glu Glu Gly Phe 
                165                 170                 175     


Arg Asp Asp Ile Gly Ser Phe Pro Asn Gly Glu Arg Ala Ile Gly Tyr 
            180                 185                 190         


Lys Gln Arg Phe Lys Met Pro Tyr Ile Gly Leu Thr Gly Ser Tyr Arg 
        195                 200                 205             


Tyr Glu Asp Phe Glu Leu Gly Gly Thr Phe Lys Tyr Ser Gly Trp Val 
    210                 215                 220                 


Glu Ser Ser Asp Asn Asp Glu His Tyr Asp Pro Gly Lys Arg Ile Thr 
225                 230                 235                 240 


Tyr Arg Ser Lys Val Lys Asp Gln Asn Tyr Tyr Ser Val Ala Val Asn 
                245                 250                 255     


Ala Gly Tyr Tyr Val Thr Pro Asn Ala Lys Val Tyr Val Glu Gly Ala 
            260                 265                 270         


Trp Asn Arg Val Thr Asn Lys Lys Gly Asn Thr Ser Leu Tyr Asp His 
        275                 280                 285             


Asn Asn Asn Thr Ser Asp Tyr Ser Lys Asn Gly Ala Gly Ile Glu Asn 
    290                 295                 300                 


Tyr Asn Phe Ile Thr Thr Ala Gly Leu Lys Tyr Thr Phe 
305                 310                 315         


<210>  11
<211>  268
<212>  PRT
<213>  E. coli

<400>  11

Met Lys Ala Phe Trp Arg Asn Ala Ala Leu Leu Ala Val Ser Leu Leu 
1               5                   10                  15      


Pro Phe Ser Ser Ala Asn Ala Leu Ala Leu Gln Ala Lys Gln Tyr Gly 
            20                  25                  30          


Asp Phe Asp Arg Tyr Val Leu Ala Leu Ser Trp Gln Thr Gly Phe Cys 
        35                  40                  45              


Gln Ser Gln His Asp Arg Asn Arg Asn Glu Arg Asp Glu Cys Arg Leu 
    50                  55                  60                  


Gln Thr Glu Thr Thr Asn Lys Ala Asp Phe Leu Thr Val His Gly Leu 
65                  70                  75                  80  


Trp Pro Gly Leu Pro Lys Ser Val Ala Ala Arg Gly Val Asp Glu Arg 
                85                  90                  95      


Arg Trp Met Arg Phe Gly Cys Ala Thr Arg Pro Ile Pro Asn Leu Pro 
            100                 105                 110         


Glu Ala Arg Ala Ser Arg Met Cys Ser Ser Pro Glu Thr Gly Leu Ser 
        115                 120                 125             


Leu Glu Thr Ala Ala Lys Leu Ser Glu Val Met Pro Gly Ala Gly Gly 
    130                 135                 140                 


Arg Ser Cys Leu Glu Arg Tyr Glu Tyr Ala Lys His Gly Ala Cys Phe 
145                 150                 155                 160 


Gly Phe Asp Pro Asp Ala Tyr Phe Gly Thr Met Val Arg Leu Asn Gln 
                165                 170                 175     


Glu Ile Lys Glu Ser Glu Ala Gly Lys Phe Leu Ala Asp Asn Tyr Gly 
            180                 185                 190         


Lys Thr Val Ser Arg Arg Asp Phe Asp Ala Ala Phe Ala Lys Ser Trp 
        195                 200                 205             


Gly Lys Glu Asn Val Lys Ala Val Lys Leu Thr Cys Gln Gly Asn Pro 
    210                 215                 220                 


Ala Tyr Leu Thr Glu Ile Gln Ile Ser Ile Lys Ala Asp Ala Ile Asn 
225                 230                 235                 240 


Ala Pro Leu Ser Ala Asn Ser Phe Leu Pro Gln Pro His Pro Gly Asn 
                245                 250                 255     


Cys Gly Lys Thr Phe Val Ile Asp Lys Ala Gly Tyr 
            260                 265             


<210>  12
<211>  644
<212>  PRT
<213>  E. coli

<400>  12

Met Phe Gln Asp Asn Pro Leu Leu Ala Gln Leu Lys Gln Gln Leu His 
1               5                   10                  15      


Ser Gln Thr Pro Arg Ala Glu Gly Val Val Lys Ala Thr Glu Lys Gly 
            20                  25                  30          


Phe Gly Phe Leu Glu Val Asp Ala Gln Lys Ser Tyr Phe Ile Pro Pro 
        35                  40                  45              


Pro Gln Met Lys Lys Val Met His Gly Asp Arg Ile Ile Ala Val Ile 
    50                  55                  60                  


His Ser Glu Lys Glu Arg Glu Ser Ala Glu Pro Glu Glu Leu Val Glu 
65                  70                  75                  80  


Pro Phe Leu Thr Arg Phe Val Gly Lys Val Gln Gly Lys Asn Asp Arg 
                85                  90                  95      


Leu Ala Ile Val Pro Asp His Pro Leu Leu Lys Asp Ala Ile Pro Cys 
            100                 105                 110         


Arg Ala Ala Arg Gly Leu Asn His Glu Phe Lys Glu Gly Asp Trp Ala 
        115                 120                 125             


Val Ala Glu Met Arg Arg His Pro Leu Lys Gly Asp Arg Ser Phe Tyr 
    130                 135                 140                 


Ala Glu Leu Thr Gln Tyr Ile Thr Phe Gly Asp Asp His Phe Val Pro 
145                 150                 155                 160 


Trp Trp Val Thr Leu Ala Arg His Asn Leu Glu Lys Glu Ala Pro Asp 
                165                 170                 175     


Gly Val Ala Thr Glu Met Leu Asp Glu Gly Leu Val Arg Glu Asp Leu 
            180                 185                 190         


Thr Ala Leu Asp Phe Val Thr Ile Asp Ser Ala Ser Thr Glu Asp Met 
        195                 200                 205             


Asp Asp Ala Leu Phe Ala Lys Ala Leu Pro Asp Asp Lys Leu Gln Leu 
    210                 215                 220                 


Ile Val Ala Ile Ala Asp Pro Thr Ala Trp Ile Ala Glu Gly Ser Lys 
225                 230                 235                 240 


Leu Asp Lys Ala Ala Lys Ile Arg Ala Phe Thr Asn Tyr Leu Pro Gly 
                245                 250                 255     


Phe Asn Ile Pro Met Leu Pro Arg Glu Leu Ser Asp Asp Leu Cys Ser 
            260                 265                 270         


Leu Arg Ala Asn Glu Val Arg Pro Val Leu Ala Cys Arg Met Thr Leu 
        275                 280                 285             


Ser Ala Asp Gly Thr Ile Glu Asp Asn Ile Glu Phe Phe Ala Ala Thr 
    290                 295                 300                 


Ile Glu Ser Lys Ala Lys Leu Val Tyr Asp Gln Val Ser Asp Trp Leu 
305                 310                 315                 320 


Glu Asn Thr Gly Asp Trp Gln Pro Glu Ser Glu Ala Ile Ala Glu Gln 
                325                 330                 335     


Val Arg Leu Leu Ala Gln Ile Cys Gln Arg Arg Gly Glu Trp Arg His 
            340                 345                 350         


Asn His Ala Leu Val Phe Lys Asp Arg Pro Asp Tyr Arg Phe Ile Leu 
        355                 360                 365             


Gly Glu Lys Gly Glu Val Leu Asp Ile Val Ala Glu Pro Arg Arg Ile 
    370                 375                 380                 


Ala Asn Arg Ile Val Glu Glu Ala Met Ile Ala Ala Asn Ile Cys Ala 
385                 390                 395                 400 


Ala Arg Val Leu Arg Asp Lys Leu Gly Phe Gly Ile Tyr Asn Val His 
                405                 410                 415     


Met Gly Phe Asp Pro Ala Asn Ala Asp Ala Leu Ala Ala Leu Leu Lys 
            420                 425                 430         


Thr His Gly Leu His Val Asp Ala Glu Glu Val Leu Thr Leu Asp Gly 
        435                 440                 445             


Phe Cys Lys Leu Arg Arg Glu Leu Asp Ala Gln Pro Thr Gly Phe Leu 
    450                 455                 460                 


Asp Ser Arg Ile Arg Arg Phe Gln Ser Phe Ala Glu Ile Ser Thr Glu 
465                 470                 475                 480 


Pro Gly Pro His Phe Gly Leu Gly Leu Glu Ala Tyr Ala Thr Trp Thr 
                485                 490                 495     


Ser Pro Ile Arg Lys Tyr Gly Asp Met Ile Asn His Arg Leu Leu Lys 
            500                 505                 510         


Ala Val Ile Lys Gly Glu Thr Ala Thr Arg Pro Gln Asp Glu Ile Thr 
        515                 520                 525             


Val Gln Met Ala Glu Arg Arg Arg Leu Asn Arg Met Ala Glu Arg Asp 
    530                 535                 540                 


Val Gly Asp Trp Leu Tyr Ala Arg Phe Leu Lys Asp Lys Ala Gly Thr 
545                 550                 555                 560 


Asp Thr Arg Phe Ala Ala Glu Ile Val Asp Ile Ser Arg Gly Gly Met 
                565                 570                 575     


Arg Val Arg Leu Val Asp Asn Gly Ala Ile Ala Phe Ile Pro Ala Pro 
            580                 585                 590         


Phe Leu His Ala Val Arg Asp Glu Leu Val Cys Ser Gln Glu Asn Gly 
        595                 600                 605             


Thr Val Gln Ile Lys Gly Glu Thr Val Tyr Lys Val Thr Asp Val Ile 
    610                 615                 620                 


Asp Val Thr Ile Ala Glu Val Arg Met Glu Thr Arg Ser Ile Ile Ala 
625                 630                 635                 640 


Arg Pro Val Ala 
                


<210>  13
<211>  502
<212>  PRT
<213>  E. coli

<400>  13

Met Thr Glu Lys Lys Tyr Ile Val Ala Leu Asp Gln Gly Thr Thr Ser 
1               5                   10                  15      


Ser Arg Ala Val Val Met Asp His Asp Ala Asn Ile Ile Ser Val Ser 
            20                  25                  30          


Gln Arg Glu Phe Glu Gln Ile Tyr Pro Lys Pro Gly Trp Val Glu His 
        35                  40                  45              


Asp Pro Met Glu Ile Trp Ala Thr Gln Ser Ser Thr Leu Val Glu Val 
    50                  55                  60                  


Leu Ala Lys Ala Asp Ile Ser Ser Asp Gln Ile Ala Ala Ile Gly Ile 
65                  70                  75                  80  


Thr Asn Gln Arg Glu Thr Thr Ile Val Trp Glu Lys Glu Thr Gly Lys 
                85                  90                  95      


Pro Ile Tyr Asn Ala Ile Val Trp Gln Cys Arg Arg Thr Ala Glu Ile 
            100                 105                 110         


Cys Glu His Leu Lys Arg Asp Gly Leu Glu Asp Tyr Ile Arg Ser Asn 
        115                 120                 125             


Thr Gly Leu Val Ile Asp Pro Tyr Phe Ser Gly Thr Lys Val Lys Trp 
    130                 135                 140                 


Ile Leu Asp His Val Glu Gly Ser Arg Glu Arg Ala Arg Arg Gly Glu 
145                 150                 155                 160 


Leu Leu Phe Gly Thr Val Asp Thr Trp Leu Ile Trp Lys Met Thr Gln 
                165                 170                 175     


Gly Arg Val His Val Thr Asp Tyr Thr Asn Ala Ser Arg Thr Met Leu 
            180                 185                 190         


Phe Asn Ile His Thr Leu Asp Trp Asp Asp Lys Met Leu Glu Val Leu 
        195                 200                 205             


Asp Ile Pro Arg Glu Met Leu Pro Glu Val Arg Arg Ser Ser Glu Val 
    210                 215                 220                 


Tyr Gly Gln Thr Asn Ile Gly Gly Lys Gly Gly Thr Arg Ile Pro Ile 
225                 230                 235                 240 


Ser Gly Ile Ala Gly Asp Gln Gln Ala Ala Leu Phe Gly Gln Leu Cys 
                245                 250                 255     


Val Lys Glu Gly Met Ala Lys Asn Thr Tyr Gly Thr Gly Cys Phe Met 
            260                 265                 270         


Leu Met Asn Thr Gly Glu Lys Ala Val Lys Ser Glu Asn Gly Leu Leu 
        275                 280                 285             


Thr Thr Ile Ala Cys Gly Pro Thr Gly Glu Val Asn Tyr Ala Leu Glu 
    290                 295                 300                 


Gly Ala Val Phe Met Ala Gly Ala Ser Ile Gln Trp Leu Arg Asp Glu 
305                 310                 315                 320 


Met Lys Leu Ile Asn Asp Ala Tyr Asp Ser Glu Tyr Phe Ala Thr Lys 
                325                 330                 335     


Val Gln Asn Thr Asn Gly Val Tyr Val Val Pro Ala Phe Thr Gly Leu 
            340                 345                 350         


Gly Ala Pro Tyr Trp Asp Pro Tyr Ala Arg Gly Ala Ile Phe Gly Leu 
        355                 360                 365             


Thr Arg Gly Val Asn Ala Asn His Ile Ile Arg Ala Thr Leu Glu Ser 
    370                 375                 380                 


Ile Ala Tyr Gln Thr Arg Asp Val Leu Glu Ala Met Gln Ala Asp Ser 
385                 390                 395                 400 


Gly Ile Arg Leu His Ala Leu Arg Val Asp Gly Gly Ala Val Ala Asn 
                405                 410                 415     


Asn Phe Leu Met Gln Phe Gln Ser Asp Ile Leu Gly Thr Arg Val Glu 
            420                 425                 430         


Arg Pro Glu Val Arg Glu Val Thr Ala Leu Gly Ala Ala Tyr Leu Ala 
        435                 440                 445             


Gly Leu Ala Val Gly Phe Trp Gln Asn Leu Asp Glu Leu Gln Glu Lys 
    450                 455                 460                 


Ala Val Ile Glu Arg Glu Phe Arg Pro Gly Ile Glu Thr Thr Glu Arg 
465                 470                 475                 480 


Asn Tyr Arg Tyr Ala Gly Trp Lys Lys Ala Val Lys Arg Ala Met Ala 
                485                 490                 495     


Trp Glu Glu His Asp Glu 
            500         


<210>  14
<211>  450
<212>  PRT
<213>  E. coli

<400>  14

Met Thr Lys His Tyr Asp Tyr Ile Ala Ile Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Ile Ala Ser Ile Asn Arg Ala Ala Met Tyr Gly Gln Lys Cys Ala Leu 
            20                  25                  30          


Ile Glu Ala Lys Glu Leu Gly Gly Thr Cys Val Asn Val Gly Cys Val 
        35                  40                  45              


Pro Lys Lys Val Met Trp His Ala Ala Gln Ile Arg Glu Ala Ile His 
    50                  55                  60                  


Met Tyr Gly Pro Asp Tyr Gly Phe Asp Thr Thr Ile Asn Lys Phe Asn 
65                  70                  75                  80  


Trp Glu Thr Leu Ile Ala Ser Arg Thr Ala Tyr Ile Asp Arg Ile His 
                85                  90                  95      


Thr Ser Tyr Glu Asn Val Leu Gly Lys Asn Asn Val Asp Val Ile Lys 
            100                 105                 110         


Gly Phe Ala Arg Phe Val Asp Ala Lys Thr Leu Glu Val Asn Gly Glu 
        115                 120                 125             


Thr Ile Thr Ala Asp His Ile Leu Ile Ala Thr Gly Gly Arg Pro Ser 
    130                 135                 140                 


His Pro Asp Ile Pro Gly Val Glu Tyr Gly Ile Asp Ser Asp Gly Phe 
145                 150                 155                 160 


Phe Ala Leu Pro Ala Leu Pro Glu Arg Val Ala Val Val Gly Ala Gly 
                165                 170                 175     


Tyr Ile Ala Val Glu Leu Ala Gly Val Ile Asn Gly Leu Gly Ala Lys 
            180                 185                 190         


Thr His Leu Phe Val Arg Lys His Ala Pro Leu Arg Ser Phe Asp Pro 
        195                 200                 205             


Met Ile Ser Glu Thr Leu Val Glu Val Met Asn Ala Glu Gly Pro Gln 
    210                 215                 220                 


Leu His Thr Asn Ala Ile Pro Lys Ala Val Val Lys Asn Thr Asp Gly 
225                 230                 235                 240 


Ser Leu Thr Leu Glu Leu Glu Asp Gly Arg Ser Glu Thr Val Asp Cys 
                245                 250                 255     


Leu Ile Trp Ala Ile Gly Arg Glu Pro Ala Asn Asp Asn Ile Asn Leu 
            260                 265                 270         


Glu Ala Ala Gly Val Lys Thr Asn Glu Lys Gly Tyr Ile Val Val Asp 
        275                 280                 285             


Lys Tyr Gln Asn Thr Asn Ile Glu Gly Ile Tyr Ala Val Gly Asp Asn 
    290                 295                 300                 


Thr Gly Ala Val Glu Leu Thr Pro Val Ala Val Ala Ala Gly Arg Arg 
305                 310                 315                 320 


Leu Ser Glu Arg Leu Phe Asn Asn Lys Pro Asp Glu His Leu Asp Tyr 
                325                 330                 335     


Ser Asn Ile Pro Thr Val Val Phe Ser His Pro Pro Ile Gly Thr Val 
            340                 345                 350         


Gly Leu Thr Glu Pro Gln Ala Arg Glu Gln Tyr Gly Asp Asp Gln Val 
        355                 360                 365             


Lys Val Tyr Lys Ser Ser Phe Thr Ala Met Tyr Thr Ala Val Thr Thr 
    370                 375                 380                 


His Arg Gln Pro Cys Arg Met Lys Leu Val Cys Val Gly Ser Glu Glu 
385                 390                 395                 400 


Lys Ile Val Gly Ile His Gly Ile Gly Phe Gly Met Asp Glu Met Leu 
                405                 410                 415     


Gln Gly Phe Ala Val Ala Leu Lys Met Gly Ala Thr Lys Lys Asp Phe 
            420                 425                 430         


Asp Asn Thr Val Ala Ile His Pro Thr Ala Ala Glu Glu Phe Val Thr 
        435                 440                 445             


Met Arg 
    450 


<210>  15
<211>  518
<212>  PRT
<213>  E. coli

<400>  15

Met Ile Pro Asp Val Ser Gln Ala Leu Ala Trp Leu Glu Lys His Pro 
1               5                   10                  15      


Gln Ala Leu Lys Gly Ile Gln Arg Gly Leu Glu Arg Glu Thr Leu Arg 
            20                  25                  30          


Val Asn Ala Asp Gly Thr Leu Ala Thr Thr Gly His Pro Glu Ala Leu 
        35                  40                  45              


Gly Ser Ala Leu Thr His Lys Trp Ile Thr Thr Asp Phe Ala Glu Ala 
    50                  55                  60                  


Leu Leu Glu Phe Ile Thr Pro Val Asp Gly Asp Ile Glu His Met Leu 
65                  70                  75                  80  


Thr Phe Met Arg Asp Leu His Arg Tyr Thr Ala Arg Asn Met Gly Asp 
                85                  90                  95      


Glu Arg Met Trp Pro Leu Ser Met Pro Cys Tyr Ile Ala Glu Gly Gln 
            100                 105                 110         


Asp Ile Glu Leu Ala Gln Tyr Gly Thr Ser Asn Thr Gly Arg Phe Lys 
        115                 120                 125             


Thr Leu Tyr Arg Glu Gly Leu Lys Asn Arg Tyr Gly Ala Leu Met Gln 
    130                 135                 140                 


Thr Ile Ser Gly Val His Tyr Asn Phe Ser Leu Pro Met Ala Phe Trp 
145                 150                 155                 160 


Gln Ala Lys Cys Gly Asp Ile Ser Gly Ala Asp Ala Lys Glu Lys Ile 
                165                 170                 175     


Ser Ala Gly Tyr Phe Arg Val Ile Arg Asn Tyr Tyr Arg Phe Gly Trp 
            180                 185                 190         


Val Ile Pro Tyr Leu Phe Gly Ala Ser Pro Ala Ile Cys Ser Ser Phe 
        195                 200                 205             


Leu Gln Gly Lys Pro Thr Ser Leu Pro Phe Glu Lys Thr Glu Cys Gly 
    210                 215                 220                 


Met Tyr Tyr Leu Pro Tyr Ala Thr Ser Leu Arg Leu Ser Asp Leu Gly 
225                 230                 235                 240 


Tyr Thr Asn Lys Ser Gln Ser Asn Leu Gly Ile Thr Phe Asn Asp Leu 
                245                 250                 255     


Tyr Glu Tyr Val Ala Gly Leu Lys Gln Ala Ile Lys Thr Pro Ser Glu 
            260                 265                 270         


Glu Tyr Ala Lys Ile Gly Ile Glu Lys Asp Gly Lys Arg Leu Gln Ile 
        275                 280                 285             


Asn Ser Asn Val Leu Gln Ile Glu Asn Glu Leu Tyr Ala Pro Ile Arg 
    290                 295                 300                 


Pro Lys Arg Val Thr Arg Ser Gly Glu Ser Pro Ser Asp Ala Leu Leu 
305                 310                 315                 320 


Arg Gly Gly Ile Glu Tyr Ile Glu Val Arg Ser Leu Asp Ile Asn Pro 
                325                 330                 335     


Phe Ser Pro Ile Gly Val Asp Glu Gln Gln Val Arg Phe Leu Asp Leu 
            340                 345                 350         


Phe Met Val Trp Cys Ala Leu Ala Asp Ala Pro Glu Met Ser Ser Ser 
        355                 360                 365             


Glu Leu Ala Cys Thr Arg Val Asn Trp Asn Arg Val Ile Leu Glu Gly 
    370                 375                 380                 


Arg Lys Pro Gly Leu Thr Leu Gly Ile Gly Cys Glu Thr Ala Gln Phe 
385                 390                 395                 400 


Pro Leu Pro Gln Val Gly Lys Asp Leu Phe Arg Asp Leu Lys Arg Val 
                405                 410                 415     


Ala Gln Thr Leu Asp Ser Ile Asn Gly Gly Glu Ala Tyr Gln Lys Val 
            420                 425                 430         


Cys Asp Glu Leu Val Ala Cys Phe Asp Asn Pro Asp Leu Thr Phe Ser 
        435                 440                 445             


Ala Arg Ile Leu Arg Ser Met Ile Asp Thr Gly Ile Gly Gly Thr Gly 
    450                 455                 460                 


Lys Ala Phe Ala Glu Ala Tyr Arg Asn Leu Leu Arg Glu Glu Pro Leu 
465                 470                 475                 480 


Glu Ile Leu Arg Glu Glu Asp Phe Val Ala Glu Arg Glu Ala Ser Glu 
                485                 490                 495     


Arg Arg Gln Gln Glu Met Glu Ala Ala Asp Thr Glu Pro Phe Ala Val 
            500                 505                 510         


Trp Leu Glu Lys His Ala 
        515             


<210>  16
<211>  471
<212>  PRT
<213>  E. coli

<400>  16

Met Glu Asn Phe Lys His Leu Pro Glu Pro Phe Arg Ile Arg Val Ile 
1               5                   10                  15      


Glu Pro Val Lys Arg Thr Thr Arg Ala Tyr Arg Glu Glu Ala Ile Ile 
            20                  25                  30          


Lys Ser Gly Met Asn Pro Phe Leu Leu Asp Ser Glu Asp Val Phe Ile 
        35                  40                  45              


Asp Leu Leu Thr Asp Ser Gly Thr Gly Ala Val Thr Gln Ser Met Gln 
    50                  55                  60                  


Ala Ala Met Met Arg Gly Asp Glu Ala Tyr Ser Gly Ser Arg Ser Tyr 
65                  70                  75                  80  


Tyr Ala Leu Ala Glu Ser Val Lys Asn Ile Phe Gly Tyr Gln Tyr Thr 
                85                  90                  95      


Ile Pro Thr His Gln Gly Arg Gly Ala Glu Gln Ile Tyr Ile Pro Val 
            100                 105                 110         


Leu Ile Lys Lys Arg Glu Gln Glu Lys Gly Leu Asp Arg Ser Lys Met 
        115                 120                 125             


Val Ala Phe Ser Asn Tyr Phe Phe Asp Thr Thr Gln Gly His Ser Gln 
    130                 135                 140                 


Ile Asn Gly Cys Thr Val Arg Asn Val Tyr Ile Lys Glu Ala Phe Asp 
145                 150                 155                 160 


Thr Gly Val Arg Tyr Asp Phe Lys Gly Asn Phe Asp Leu Glu Gly Leu 
                165                 170                 175     


Glu Arg Gly Ile Glu Glu Val Gly Pro Asn Asn Val Pro Tyr Ile Val 
            180                 185                 190         


Ala Thr Ile Thr Ser Asn Ser Ala Gly Gly Gln Pro Val Ser Leu Ala 
        195                 200                 205             


Asn Leu Lys Ala Met Tyr Ser Ile Ala Lys Lys Tyr Asp Ile Pro Val 
    210                 215                 220                 


Val Met Asp Ser Ala Arg Phe Ala Glu Asn Ala Tyr Phe Ile Lys Gln 
225                 230                 235                 240 


Arg Glu Ala Glu Tyr Lys Asp Trp Thr Ile Glu Gln Ile Thr Arg Glu 
                245                 250                 255     


Thr Tyr Lys Tyr Ala Asp Met Leu Ala Met Ser Ala Lys Lys Asp Ala 
            260                 265                 270         


Met Val Pro Met Gly Gly Leu Leu Cys Met Lys Asp Asp Ser Phe Phe 
        275                 280                 285             


Asp Val Tyr Thr Glu Cys Arg Thr Leu Cys Val Val Gln Glu Gly Phe 
    290                 295                 300                 


Pro Thr Tyr Gly Gly Leu Glu Gly Gly Ala Met Glu Arg Leu Ala Val 
305                 310                 315                 320 


Gly Leu Tyr Asp Gly Met Asn Leu Asp Trp Leu Ala Tyr Arg Ile Ala 
                325                 330                 335     


Gln Val Gln Tyr Leu Val Asp Gly Leu Glu Glu Ile Gly Val Val Cys 
            340                 345                 350         


Gln Gln Ala Gly Gly His Ala Ala Phe Val Asp Ala Gly Lys Leu Leu 
        355                 360                 365             


Pro His Ile Pro Ala Asp Gln Phe Pro Ala Gln Ala Leu Ala Cys Glu 
    370                 375                 380                 


Leu Tyr Lys Val Ala Gly Ile Arg Ala Val Glu Ile Gly Ser Phe Leu 
385                 390                 395                 400 


Leu Gly Arg Asp Pro Lys Thr Gly Lys Gln Leu Pro Cys Pro Ala Glu 
                405                 410                 415     


Leu Leu Arg Leu Thr Ile Pro Arg Ala Thr Tyr Thr Gln Thr His Met 
            420                 425                 430         


Asp Phe Ile Ile Glu Ala Phe Lys His Val Lys Glu Asn Ala Ala Asn 
        435                 440                 445             


Ile Lys Gly Leu Thr Phe Thr Tyr Glu Pro Lys Val Leu Arg His Phe 
    450                 455                 460                 


Thr Ala Lys Leu Lys Glu Val 
465                 470     


<210>  17
<211>  696
<212>  DNA
<213>  V. natriegens

<400>  17
atgaaatacc tgttctcttt attcattctt gcactatcca gtgccgccgt ggccgcgcca       60

ccaagttcat tttcagccgc taagcgcgaa gcggtaaaaa tctatcaaga tcatcccacc      120

agcttttatt gcggctgtga tattcaatgg caaggcaaga aaggcttacc tgatctttcc      180

tcttgtggtt accaggttcg caaacaagaa aagcgtgctt cacgcatcga gtgggaacat      240

gtcgttccag cttggcaatt tgggcaccag ctgcaatgct ggcaaagcgg tggtcgtaaa      300

aactgctcgc gtaatgacaa aacattccgc tcaatggaag ccgatctgca caacctgact      360

cctgcgattg gtgaggtaaa tggtgatcgc tctaactaca atttcagtca gtggaatggg      420

atcgatggcg caacctatgg tcgttgtgaa gtccaggtaa acttcaagca acgcaaagtc      480

atgccacccg atcgagcacg cggctccatc gctcgtacct atctttatat gagcaaggag      540

tacggcttca aactgtccaa gcaacaaact cagttaatga gtgcatggaa caaaacctac      600

ccagccgata aatgggaatg cgaacgcgat aagcgcattg ccaaagtaca aggcaaccat      660

aatccattcg ttcaagaggc ctgtcgcgca ctgtaa                                696


<210>  18
<211>  2352
<212>  DNA
<213>  V. natriegens

<400>  18
atgaacttgg aacgttccga gagtatcgag atcccggtac tacctctacg tgacgtagtg       60

gtctacccgc acatggtcat tccattgttt gttggtcgtg aaaaatcgat tagctgtcta      120

gaaacggcga tggaaacaaa caaacaagtt ctgcttgtgg cacaaaagca agcggataca      180

gacgagccaa cggttgacga cctatttgag gtaggtacgg tagccaccat tcttcagctt      240

ttaaagcttc ctgatggcac agtaaaagta ctggttgaag gtcagcagcg tgcgaagatt      300

aatcacttta aagagagcga ttttttctta gctgaagctg aattcatcgt gacacctgag      360

ctggatgagc gtgagcaaga agttattgtt cgtagtgcga tcaaccagtt cgaaggcttt      420

atcaagctga acaaaaaaat cccaccagaa gttctgactt cgctaaatgg gattgatgaa      480

gccgcgcgtc tagccgatac catcgcagct cacatgcctt tgaaattggt cgacaagcaa      540

caagtacttg agatcataga cgttaccgag cgtttggaat tcctgatggg ccaaatggag      600

tcggaaatcg atctgctgca agttgaaaaa cgcatccgtg gccgcgttaa aaagcaaatg      660

gagaagtctc agcgcgagta ctatctgaat gagcaaatga aagcgattca gaaagagcta      720

ggtgagatgg aagatgcgcc ggatgaattt gaaacgctgc agaataaaat cgaagagtcc      780

aaaatgcctc aagaggcgcg cgaaaagaca gagcaagagc tacaaaagct taagatgatg      840

tctccaatgt ctgcggaagc aacagtggtg cgtagctaca tcgattggat ggtgagcgtt      900

ccttgggcta agcgttctaa agtgaaaaag aacctggcta aggcagaaga gattctaaac      960

gaagatcatt acggtctgga gcgcgtcaaa gagcgcattc tggaatactt ggcagtacaa     1020

aaccgtatta acaagctgaa aggcccaatc ctttgtcttg ttggtcctcc aggtgtgggt     1080

aaaacctctc ttggccgttc gatcgcatct gcgactgggc gtaaatacgt gcgtatggcg     1140

cttggtggtg tgcgtgacga agctgagatt cgtggccacc gtcgtactta cattggctca     1200

ctaccgggta agcttatcca gaaaatgtct aaagttgggg ttaaaaaccc gctattcctt     1260

cttgatgaga tcgataagat gtcttctgac atgcgcggtg atccagcatc tgcactgcta     1320

gaagttctgg atcctgaaca aaacaactcg ttcaacgatc actacttaga agtagattac     1380

gatctgtcgg atgtaatgtt cgttgcgacg tctaactcga tgaatatccc aggtccgctt     1440

cttgaccgta tggaagttat ccgtctttct ggttacacag aagatgaaaa actgaacatc     1500

gcgaaacgcc acttggtaga taagcagttg aagcgtaacg gactgaagcc aaacgagatt     1560

gttatcgagg actcagcgat tgtcggcatt attcgttact acactcgtga agcgggtgta     1620

cgtaacctag agcgtgaaat ttctaagatc tgtcgtaagg cggtgaagaa tatcctgctg     1680

gataaagata tcaagtctgt gaccgtatct atggacaacc tgaaagagta cctgggtgtt     1740

cagcgttttg actatggtaa agctgatgaa agcaaccgta ttggtcaggt gacgggtttg     1800

gcttggactg aagtcggtgg tgatttgcta actattgaaa ctcagtctat gccgggtaaa     1860

ggtaaactga cacagacggg gtctcttggc gacgtgatgc aagagtctat ccaagcggcc     1920

atgacagttg ttcgctctcg tgctgaaaag ctgggtatca acaccgattt ctacgagaag     1980

aaagacatcc acgtgcatgt tcctgaaggt gcgacaccaa aagatggccc aagtgctggt     2040

acagcaatgt gcactgcttt ggtttctgcg ttaactggta acccagtgaa agcggaagtg     2100

gcaatgacgg gtgaaatcac actacgtggt gaagttttgc ctatcggtgg cctaaaagaa     2160

aaattacttg cggcacatcg tggcggcatt aaaacggtac tgattccaaa agataacgag     2220

cgtgatttgg aagagattcc agagaatgtt atcgcagatc tgacagttat cccggttcag     2280

tggattgatg aagtactgaa agttgcactc gagcgagacc cgacgggcgt tgagtttgaa     2340

gctaaaaaat ag                                                         2352


<210>  19
<211>  2001
<212>  DNA
<213>  V. natriegens

<400>  19
atgtttcaag ataacccgct attagcccaa cttaagcagc aaatccaaga aaaccttcct       60

aaaaaagaag ggtcaatcaa agcaacagat aaaggctttg gtttccttga agtggacagc      120

aaaaccagtt tcttcattcc acctgcgtac atgaaaaagt gcattcatgg cgataaagta      180

gtcgccatta ttcgtacaga gaatgaacgt gaagttgcag agccacaaga gctgctagaa      240

cagtcactta ctcgctttat tggccgtgta aaaatgttca aaggtaagct gaatgttgtt      300

cctgaccacc ctcaactgaa aaagttgtcg ctaaaagcaa aactaaagaa aggccaaaag      360

ccagataact tcaatgaagg tgattgggtt gtcggccatc ttattcgcca ccctcttaaa      420

ggtgacaact ctttcttcgt agaaatctct gaaaaaatca cggatgctga cgataagatc      480

gcaccatggt gggtaacctt ggcacaaaac gatcttccaa actctgagcc tgctggtatt      540

gagaactggg agttaaaaga cgacgcggaa ttagaccgcg ttgatttaac tcacgttcca      600

ttcgtaacta tcgatggcga gtccaccaaa gatatggacg atgcgctgca tgcgaagaaa      660

acagaatctg gcgattttga actgaccatc gcgattgccg atcctaccgc atacattacg      720

ccagaagatg aaatggacaa agtcgctcgt gagcgtggct acacgatcta cttgccaggg      780

cgcaacatcc caatgctgcc tcgcgatctt gctgatgacc tatgttctct tattgaaggt      840

gaaactcgcc ctgccctttg ctgtaccgta agcgtaagta aagatggtgt gattggtgat      900

aacatcaact tctttgctgc gaacattaag tctcacgctc gtcttgctta tgaccacgta      960

tcagactgga tcgaaaatgg cagctctgat aaatggcagc catcggaaga tatcgcaact     1020

atcgtacgtg acctgtacga cttctccgtt gctcgtgccg actggcgtga aaagaacgcg     1080

gttgtattcc cggaccgtcc ggactaccgt ttcgaactta gcgaagacaa tgacgttatt     1140

gccattcatg cagacatgcg tcgcagtgca aaccgtcttg tagaagagtc gatgatcaca     1200

gcgaatatct gtgctggccg cacgcttcgc gataagtttg aaactggcgt gttcaacact     1260

cactctggtc tcaaagcaga gaagattgaa gaagttgtac aattagttga cccagaaggt     1320

acacatggct tcacagcgga cactatcgcg acactggaag gctttgcagc attacgtcgt     1380

tggttgtcta ctcaggaaac ttcatacttg gataaccgca tccgcaagtt ccaggcgtac     1440

agtgaagtcg gcaatcagcc acttcctcac tacgcgatgg gcctagatat ttacgcgact     1500

tggacatctc caattcgtaa atacggcgat atgatcaacc accgtatgct aaaagcggtg     1560

attcttgaca aagaacctgt tcaaaagcct gatgatcaag tcggtgaaga actggcatta     1620

caccgtaagc accacaaaat tgcggagcgt aatgtgtctg actggcttta cgctcgtact     1680

cttgctgagg agccaagcaa acaaactcgc tacatcggtg aaattttcga tatcaatcgt     1740

gctggtgcgc gtgttcgcct gcttgaaaac ggcgccgcgg cgtttattcc tggctcactc     1800

attgttgaca ataaagaacg cattgagtgc aacggtgaca acggtactat ctccatcgat     1860

aaagaagtgg tgtacaagct gggcgatacg ctagaagttg tcttggcaga tgtgaaccag     1920

gaaaaccgca gtttagttgc taaaccgacg caagtgttcg ctgagccgcc aaaagcacag     1980

acggaacaaa cggttgaata a                                               2001


<210>  20
<211>  1518
<212>  DNA
<213>  V. natriegens

<400>  20
atgactgagc aaaaatacat tgttgcccta gaccaaggta cgacaagctc tcgcgcagta       60

attcttgatc acgacgcgaa tatcgtcagc gtcgctcagc gcgaatttac tcagatttat      120

ccgcaagcag gttgggttga gcacgatcca atggaaatct gggcaacaca aagttcaacc      180

ttagttgaag ctttagctaa gtcaggcatc cgcagcgatc aattagcggc tattggcatc      240

acaaaccaac gtgaaacaac gatcgtctgg aacaaagaaa caggtaaacc ggtttacaac      300

gctatcgtct ggcaatgtcg acgcaccgca gaaatttgcg aagacttaaa acgccgcggc      360

ttagaagact acgtacgtga caacacaggc ttagtgcttg acccttactt ctctggcacc      420

aaagtgaagt ggattcttga taacgtcgaa ggtgcccgcg aagatgccga agcaggaaaa      480

ctgttatttg gtacggttga tacctggctg gtgtggaaaa tgacacaagg ccgtgtgcac      540

gtaacggatt acaccaacgc ttcacgtacg atgctattta atatcaatga cctatgttgg      600

gaccaaaagc tgttggatga gttgggtatt cctgcgtcaa tgatgcctga agtaaaacgt      660

tcatctgaaa tctatggcaa aaccaacatt ggtggtaaag gtggtacgcg tattcctatt      720

gcgggtattg ctggtgacca acaagctgcg ctatacggcc agatgtgtgt tgaagcaggt      780

caggcaaaga acacctacgg tacaggttgc ttcttgttga tgaatactgg tcaggaaaaa      840

gtgacgtcta cacacggcct gctgacaaca ctggcatgtg gcccgaaagg tgaaccggca      900

tacgcactag aaggcgcggt gttcatgggt ggtgcgtcta ttcagtggct gcgtgacgag      960

cttaagattc ttaatggcgc agaagactct gaatacttcg caacaaaagt agacacatcc     1020

aatggtgtgt acgtcgtgcc agcctttact ggccttggcg caccatactg ggatgcgtac     1080

gcacgaggaa ccattgttgg cttaactcga ggcgttaact caaaccatat tattcgtgcg     1140

actttggaag gtatcgctta ccaaacccgt gacgtattgg atgcaatgca agccgactct     1200

ggaatccagt tggctaacct aagagttgac ggcggcgcag tagcaaacaa cttcttgatg     1260

caattccagt ctgacgtact aaatacgcaa gtacttcgcc ctgaagtcac tgaagtaacc     1320

gctctgggtg cagcttatct ggcaggctta gcggtaggat attgggatag cctcgatgaa     1380

ctgcaaggta aagcggtaat tgatcgtaca tttgagcctc atgatgatga agagaagcgt     1440

aatcgtcgct acaaaggctg gaagcgtgca gtgaaatgtg ctcaaacttg gtcagaactt     1500

cacgacgaag acgattaa                                                   1518


<210>  21
<211>  1356
<212>  DNA
<213>  V. natriegens

<400>  21
atggcgactc attttgacta tatctgtatc ggtggcggca gtggcggcat cgcatctgca       60

aaccgtgcag ccatgtacgg cgcgaaagta gcgctgattg aagcacaaga ccttggtggc      120

acctgtgtaa acgtaggttg tgtaccaaag aaagtgatgt ggcatggcgc gcaaatcgca      180

gaagcaatga acctgtacgc ggaagattat gggtttgatg tcgacgtgaa aggtttcgac      240

tggagcaagc tggtagagag tcgtcaggcg tacattggtc gtattcacca atcttacgac      300

cgtgttctag gcaacaacaa agtaaatgtt atcaaaggct ttgctaagtt tgttgacgaa      360

aaaaccgttg aagtaaacgg tgaacactac acggccgatc atatcctgat cgctgttggt      420

ggccgtccaa ctattccaaa catcccgggc gcagaatacg gcatcgattc aaacggcttc      480

ttcgacctgg ctgagcaacc aaaacgcgta gcggttgttg gtgcaggcta catcgcagtt      540

gaaatcgcgg gcgtgctaca cgcactaggt acagaaacac acctgttcgt acgtaaagaa      600

tcgccactgc gtagcttcga tccaatgatc atcgaaacgt tggttgaagt gatggacgct      660

gaaggtccaa aactgcacac ccattctgta ccaaaagaaa tcattaaaga agcagatggc      720

actctgactc tgcacctgga aaatggtgaa agccaaaacg ttgaccagct aatctgggca      780

atcggccgtc acccaacaac agacgctatc aacctagcat caactggcgt tgcaaccaac      840

gacaaaggct acatcaaggt agacgaatac caagaaacca acgtgaaagg catctactgt      900

gtaggtgaca tcatggaagg tggtatcgag ctaacacctg tagcagtgaa agcaggtcgt      960

cagctttctg agcgtttgtt caacaacaag ccaaacgcga agatggatta cgacctcgtt     1020

ccgactgtcg tattcagcca cccaccaatt ggtaccatcg gtctgactga gccagaagcg     1080

attgcgaagt acggcgaaga caacgtgaaa gtgtacacct ctggcttcac tgcaatgtac     1140

accgcggtca ctaagcaccg tcaaccatgt aagatgaagc tggtatgtgc tggtgaagaa     1200

gaaacggtag tcggcctgca cggcatcggt ttcacggtag acgaaatgat tcagggcttt     1260

ggcgtagcaa tgaagatggg tgcaactaaa gcagacttcg actctgttgt agcaattcac     1320

ccaacgggct cggaagagtt cgttactatg agatag                               1356


<210>  22
<211>  1569
<212>  DNA
<213>  V. natriegens

<400>  22
ttgactgatt ttgctgcgcg actggaaaaa gttgcatcaa acccggaagt atttaaacag       60

tttggacgcg gtgttgagcg tgaaacgtta cgctatcgtc aggatggaca gctagcaaca      120

acacctcatc cagagggatt gggctcagcg ttcacaaacc agtggattac cacggacttt      180

tcagagtcgt tactggagtt cattactccg gtttctcatg atgttccgga gctaatggca      240

caactgaaag atattcatca ctttactcaa actaaaatgg gtgaagaaaa aatgtggccg      300

ctttctatgc catgttatgt cgccagtgaa gataatatta atctggcaca gtacggatcg      360

tctaacgcag ctcgaatgaa aacgctctac cgagagggtt tgaaacgccg ttatggcagc      420

ttaatgcaga tcatttcggg tgttcacttc aacttctcgt tcccagaatc gttttgggat      480

gccctatatg gtgagcagga tgaagaggct cgtcaggaaa ccaaatccga tgcctatttt      540

gccctcattc gtaactacta tcgttttggt tggatgattc cctacttctt tggtgcttcg      600

cctgcgttgt gtggttcgtt tattcaaggc cgagaaacaa gcttaccgtt tgagagcttg      660

ggtggaacgt tattcttacc gaaatcaacg tctttgcgtc tgagcgacct tggttacacg      720

aataatgcac agagttcgct aaagattggc ttcaatagta ttgaccagta tctggaaggt      780

ttaagtgatg ctattcgtcg tccgtcagaa gagttcgcaa aaattggtgt gaaagttgat      840

ggcgagtacc gtcagctcaa ttcgaatgtg ttgcaaatag aaaacgaatt gtacgcgcca      900

attcgcccta aacgagtggc taaaagtggt gagaaaccat cagaagcgtt aaagcgtgcg      960

ggtgttgaat atattgaagt tcgttcattg gacgtgaacc cattcagccc agtaggcata     1020

actgaagagc aagttcgctt cctcgacctg ttcctgactt gggcagcact gtcagactca     1080

gaaccaatgg ataactgtga actggagtgt tggcgtgata actggaacaa agtcattgta     1140

tcgggccgtg aaaaaggctt gatgcttcag atcggttgcc aaggtgagcg cttacctcta     1200

caagagtggg ctcaccgcgt gtttgcagat ctacgccaaa ttgctgtgat gatggatgaa     1260

gtgaacggtg ataatgctta ccaagaggtt tgcgataagt taactggctg gattgatgaa     1320

cctgaattga cgacttctgg tcaattgctg gaactgacca aggagttggg cggcttaggt     1380

aaagtaggtt gttcacttgg tatgaagcat cgtgaagata acctgaatca cggctaccag     1440

cattactcac aagaagtgat ggaacaggaa gcgttagcat cggttgaaaa gcaaaagcaa     1500

gcagagctaa gtgacacgat gtcttttgat gacttcctgg aagactattt ttcttattta     1560

aaacaataa                                                             1569


<210>  23
<211>  1461
<212>  DNA
<213>  V. natriegens

<400>  23
atggaaaact ttaaacactt acctgagccg tttcgtattc gcgttattga accagtaaaa       60

cgcactacac gtgaatatag agaagaagcg attctaaaag cgggtatgaa cccttttctt      120

ctggatagcg aagatgtgtt tatcgacctt cttaccgata gtggtacagg cgctattacg      180

caagagatgc aagcagccat gttgcgcggc gatgaagcat acagcggcag ccgaagctac      240

catgcacttc aacaggccgt tgaggatatc tttggctacc aatacaccat tccaactcac      300

caaggacgtg gtgcagagca aatttacatt ccggttctga ttaaaaaacg tgagaaagag      360

aaagaactag atagaagcaa gatggttgcg ctgtcgaatt acttttttga caccacacaa      420

ggccatacac agttaaactg ctgtgttgcg aaaaacgttt ataccgaaga agcattcgac      480

acttcaatcg aagcagactt taaaggtaac tttgatctgg agaaactaga acaggcaatc      540

ctagaagctg gcgcagcaaa tgtaccttat attgtcagca ccatcacatg taactctgcc      600

ggcggccagc cagtatcgct tgctaaccta aaagcggttt atgaaattgg tcagaaatac      660

gatattccag tgatcatgga ttcggctcgt tttgccgaga atgcttactt cattcaacaa      720

cgtgaagctg gctacgctga ctggtcaatc gaagcaatca ccaaagagag ctacaaatac      780

gcggatggtc tggcgatgtc ggcaaagaaa gatgccatgg ttcaaatggg cggcttactt      840

tgctttaaag acgattcgtt aatggacgtg tacacggaat gccgcacgct gtgtgttgtt      900

caggaaggct ttccgaccta tggtggtctg gaaggtggcg ctatggagcg tcttgccgta      960

ggcctttatg atggcatgcg tcaggactgg ctggcatacc gaatcggtca ggttcagtac     1020

ctggttgata agctggaagc gattggcatc gtttgtcagc aagcgggtgg ccatgctgcc     1080

ttcgttgacg caagcaaact cctgcctcac attcctgcag agcaattccc ggcacatgca     1140

ctggcgtgcg agctttataa agtcgctggt attcgagcgg tagaaatagg ctcccttcta     1200

ttgggccgag atcctgcgac aggtaaacaa cacccgtgtc cggctgaact gcttcgcctt     1260

accattcctc gcgctaccta cacacaaacg catatggatt tcattgtcga agcattccag     1320

gcggtaaaag aaaatgcagc caatgtaaaa ggtctggact ttacttatga accggaagtt     1380

ttacgtcact tcaccgcgcg tctaaaagag gttgagtcag agactcaagc tccatccatc     1440

acgacagcag aaacagtata a                                               1461


<210>  24
<211>  231
<212>  PRT
<213>  V. natriegens

<400>  24

Met Lys Tyr Leu Phe Ser Leu Phe Ile Leu Ala Leu Ser Ser Ala Ala 
1               5                   10                  15      


Val Ala Ala Pro Pro Ser Ser Phe Ser Ala Ala Lys Arg Glu Ala Val 
            20                  25                  30          


Lys Ile Tyr Gln Asp His Pro Thr Ser Phe Tyr Cys Gly Cys Asp Ile 
        35                  40                  45              


Gln Trp Gln Gly Lys Lys Gly Leu Pro Asp Leu Ser Ser Cys Gly Tyr 
    50                  55                  60                  


Gln Val Arg Lys Gln Glu Lys Arg Ala Ser Arg Ile Glu Trp Glu His 
65                  70                  75                  80  


Val Val Pro Ala Trp Gln Phe Gly His Gln Leu Gln Cys Trp Gln Ser 
                85                  90                  95      


Gly Gly Arg Lys Asn Cys Ser Arg Asn Asp Lys Thr Phe Arg Ser Met 
            100                 105                 110         


Glu Ala Asp Leu His Asn Leu Thr Pro Ala Ile Gly Glu Val Asn Gly 
        115                 120                 125             


Asp Arg Ser Asn Tyr Asn Phe Ser Gln Trp Asn Gly Ile Asp Gly Ala 
    130                 135                 140                 


Thr Tyr Gly Arg Cys Glu Val Gln Val Asn Phe Lys Gln Arg Lys Val 
145                 150                 155                 160 


Met Pro Pro Asp Arg Ala Arg Gly Ser Ile Ala Arg Thr Tyr Leu Tyr 
                165                 170                 175     


Met Ser Lys Glu Tyr Gly Phe Lys Leu Ser Lys Gln Gln Thr Gln Leu 
            180                 185                 190         


Met Ser Ala Trp Asn Lys Thr Tyr Pro Ala Asp Lys Trp Glu Cys Glu 
        195                 200                 205             


Arg Asp Lys Arg Ile Ala Lys Val Gln Gly Asn His Asn Pro Phe Val 
    210                 215                 220                 


Gln Glu Ala Cys Arg Ala Leu 
225                 230     


<210>  25
<211>  783
<212>  PRT
<213>  V. natriegens

<400>  25

Met Asn Leu Glu Arg Ser Glu Ser Ile Glu Ile Pro Val Leu Pro Leu 
1               5                   10                  15      


Arg Asp Val Val Val Tyr Pro His Met Val Ile Pro Leu Phe Val Gly 
            20                  25                  30          


Arg Glu Lys Ser Ile Ser Cys Leu Glu Thr Ala Met Glu Thr Asn Lys 
        35                  40                  45              


Gln Val Leu Leu Val Ala Gln Lys Gln Ala Asp Thr Asp Glu Pro Thr 
    50                  55                  60                  


Val Asp Asp Leu Phe Glu Val Gly Thr Val Ala Thr Ile Leu Gln Leu 
65                  70                  75                  80  


Leu Lys Leu Pro Asp Gly Thr Val Lys Val Leu Val Glu Gly Gln Gln 
                85                  90                  95      


Arg Ala Lys Ile Asn His Phe Lys Glu Ser Asp Phe Phe Leu Ala Glu 
            100                 105                 110         


Ala Glu Phe Ile Val Thr Pro Glu Leu Asp Glu Arg Glu Gln Glu Val 
        115                 120                 125             


Ile Val Arg Ser Ala Ile Asn Gln Phe Glu Gly Phe Ile Lys Leu Asn 
    130                 135                 140                 


Lys Lys Ile Pro Pro Glu Val Leu Thr Ser Leu Asn Gly Ile Asp Glu 
145                 150                 155                 160 


Ala Ala Arg Leu Ala Asp Thr Ile Ala Ala His Met Pro Leu Lys Leu 
                165                 170                 175     


Val Asp Lys Gln Gln Val Leu Glu Ile Ile Asp Val Thr Glu Arg Leu 
            180                 185                 190         


Glu Phe Leu Met Gly Gln Met Glu Ser Glu Ile Asp Leu Leu Gln Val 
        195                 200                 205             


Glu Lys Arg Ile Arg Gly Arg Val Lys Lys Gln Met Glu Lys Ser Gln 
    210                 215                 220                 


Arg Glu Tyr Tyr Leu Asn Glu Gln Met Lys Ala Ile Gln Lys Glu Leu 
225                 230                 235                 240 


Gly Glu Met Glu Asp Ala Pro Asp Glu Phe Glu Thr Leu Gln Asn Lys 
                245                 250                 255     


Ile Glu Glu Ser Lys Met Pro Gln Glu Ala Arg Glu Lys Thr Glu Gln 
            260                 265                 270         


Glu Leu Gln Lys Leu Lys Met Met Ser Pro Met Ser Ala Glu Ala Thr 
        275                 280                 285             


Val Val Arg Ser Tyr Ile Asp Trp Met Val Ser Val Pro Trp Ala Lys 
    290                 295                 300                 


Arg Ser Lys Val Lys Lys Asn Leu Ala Lys Ala Glu Glu Ile Leu Asn 
305                 310                 315                 320 


Glu Asp His Tyr Gly Leu Glu Arg Val Lys Glu Arg Ile Leu Glu Tyr 
                325                 330                 335     


Leu Ala Val Gln Asn Arg Ile Asn Lys Leu Lys Gly Pro Ile Leu Cys 
            340                 345                 350         


Leu Val Gly Pro Pro Gly Val Gly Lys Thr Ser Leu Gly Arg Ser Ile 
        355                 360                 365             


Ala Ser Ala Thr Gly Arg Lys Tyr Val Arg Met Ala Leu Gly Gly Val 
    370                 375                 380                 


Arg Asp Glu Ala Glu Ile Arg Gly His Arg Arg Thr Tyr Ile Gly Ser 
385                 390                 395                 400 


Leu Pro Gly Lys Leu Ile Gln Lys Met Ser Lys Val Gly Val Lys Asn 
                405                 410                 415     


Pro Leu Phe Leu Leu Asp Glu Ile Asp Lys Met Ser Ser Asp Met Arg 
            420                 425                 430         


Gly Asp Pro Ala Ser Ala Leu Leu Glu Val Leu Asp Pro Glu Gln Asn 
        435                 440                 445             


Asn Ser Phe Asn Asp His Tyr Leu Glu Val Asp Tyr Asp Leu Ser Asp 
    450                 455                 460                 


Val Met Phe Val Ala Thr Ser Asn Ser Met Asn Ile Pro Gly Pro Leu 
465                 470                 475                 480 


Leu Asp Arg Met Glu Val Ile Arg Leu Ser Gly Tyr Thr Glu Asp Glu 
                485                 490                 495     


Lys Leu Asn Ile Ala Lys Arg His Leu Val Asp Lys Gln Leu Lys Arg 
            500                 505                 510         


Asn Gly Leu Lys Pro Asn Glu Ile Val Ile Glu Asp Ser Ala Ile Val 
        515                 520                 525             


Gly Ile Ile Arg Tyr Tyr Thr Arg Glu Ala Gly Val Arg Asn Leu Glu 
    530                 535                 540                 


Arg Glu Ile Ser Lys Ile Cys Arg Lys Ala Val Lys Asn Ile Leu Leu 
545                 550                 555                 560 


Asp Lys Asp Ile Lys Ser Val Thr Val Ser Met Asp Asn Leu Lys Glu 
                565                 570                 575     


Tyr Leu Gly Val Gln Arg Phe Asp Tyr Gly Lys Ala Asp Glu Ser Asn 
            580                 585                 590         


Arg Ile Gly Gln Val Thr Gly Leu Ala Trp Thr Glu Val Gly Gly Asp 
        595                 600                 605             


Leu Leu Thr Ile Glu Thr Gln Ser Met Pro Gly Lys Gly Lys Leu Thr 
    610                 615                 620                 


Gln Thr Gly Ser Leu Gly Asp Val Met Gln Glu Ser Ile Gln Ala Ala 
625                 630                 635                 640 


Met Thr Val Val Arg Ser Arg Ala Glu Lys Leu Gly Ile Asn Thr Asp 
                645                 650                 655     


Phe Tyr Glu Lys Lys Asp Ile His Val His Val Pro Glu Gly Ala Thr 
            660                 665                 670         


Pro Lys Asp Gly Pro Ser Ala Gly Thr Ala Met Cys Thr Ala Leu Val 
        675                 680                 685             


Ser Ala Leu Thr Gly Asn Pro Val Lys Ala Glu Val Ala Met Thr Gly 
    690                 695                 700                 


Glu Ile Thr Leu Arg Gly Glu Val Leu Pro Ile Gly Gly Leu Lys Glu 
705                 710                 715                 720 


Lys Leu Leu Ala Ala His Arg Gly Gly Ile Lys Thr Val Leu Ile Pro 
                725                 730                 735     


Lys Asp Asn Glu Arg Asp Leu Glu Glu Ile Pro Glu Asn Val Ile Ala 
            740                 745                 750         


Asp Leu Thr Val Ile Pro Val Gln Trp Ile Asp Glu Val Leu Lys Val 
        755                 760                 765             


Ala Leu Glu Arg Asp Pro Thr Gly Val Glu Phe Glu Ala Lys Lys 
    770                 775                 780             


<210>  26
<211>  666
<212>  PRT
<213>  V. natriegens

<400>  26

Met Phe Gln Asp Asn Pro Leu Leu Ala Gln Leu Lys Gln Gln Ile Gln 
1               5                   10                  15      


Glu Asn Leu Pro Lys Lys Glu Gly Ser Ile Lys Ala Thr Asp Lys Gly 
            20                  25                  30          


Phe Gly Phe Leu Glu Val Asp Ser Lys Thr Ser Phe Phe Ile Pro Pro 
        35                  40                  45              


Ala Tyr Met Lys Lys Cys Ile His Gly Asp Lys Val Val Ala Ile Ile 
    50                  55                  60                  


Arg Thr Glu Asn Glu Arg Glu Val Ala Glu Pro Gln Glu Leu Leu Glu 
65                  70                  75                  80  


Gln Ser Leu Thr Arg Phe Ile Gly Arg Val Lys Met Phe Lys Gly Lys 
                85                  90                  95      


Leu Asn Val Val Pro Asp His Pro Gln Leu Lys Lys Leu Ser Leu Lys 
            100                 105                 110         


Ala Lys Leu Lys Lys Gly Gln Lys Pro Asp Asn Phe Asn Glu Gly Asp 
        115                 120                 125             


Trp Val Val Gly His Leu Ile Arg His Pro Leu Lys Gly Asp Asn Ser 
    130                 135                 140                 


Phe Phe Val Glu Ile Ser Glu Lys Ile Thr Asp Ala Asp Asp Lys Ile 
145                 150                 155                 160 


Ala Pro Trp Trp Val Thr Leu Ala Gln Asn Asp Leu Pro Asn Ser Glu 
                165                 170                 175     


Pro Ala Gly Ile Glu Asn Trp Glu Leu Lys Asp Asp Ala Glu Leu Asp 
            180                 185                 190         


Arg Val Asp Leu Thr His Val Pro Phe Val Thr Ile Asp Gly Glu Ser 
        195                 200                 205             


Thr Lys Asp Met Asp Asp Ala Leu His Ala Lys Lys Thr Glu Ser Gly 
    210                 215                 220                 


Asp Phe Glu Leu Thr Ile Ala Ile Ala Asp Pro Thr Ala Tyr Ile Thr 
225                 230                 235                 240 


Pro Glu Asp Glu Met Asp Lys Val Ala Arg Glu Arg Gly Tyr Thr Ile 
                245                 250                 255     


Tyr Leu Pro Gly Arg Asn Ile Pro Met Leu Pro Arg Asp Leu Ala Asp 
            260                 265                 270         


Asp Leu Cys Ser Leu Ile Glu Gly Glu Thr Arg Pro Ala Leu Cys Cys 
        275                 280                 285             


Thr Val Ser Val Ser Lys Asp Gly Val Ile Gly Asp Asn Ile Asn Phe 
    290                 295                 300                 


Phe Ala Ala Asn Ile Lys Ser His Ala Arg Leu Ala Tyr Asp His Val 
305                 310                 315                 320 


Ser Asp Trp Ile Glu Asn Gly Ser Ser Asp Lys Trp Gln Pro Ser Glu 
                325                 330                 335     


Asp Ile Ala Thr Ile Val Arg Asp Leu Tyr Asp Phe Ser Val Ala Arg 
            340                 345                 350         


Ala Asp Trp Arg Glu Lys Asn Ala Val Val Phe Pro Asp Arg Pro Asp 
        355                 360                 365             


Tyr Arg Phe Glu Leu Ser Glu Asp Asn Asp Val Ile Ala Ile His Ala 
    370                 375                 380                 


Asp Met Arg Arg Ser Ala Asn Arg Leu Val Glu Glu Ser Met Ile Thr 
385                 390                 395                 400 


Ala Asn Ile Cys Ala Gly Arg Thr Leu Arg Asp Lys Phe Glu Thr Gly 
                405                 410                 415     


Val Phe Asn Thr His Ser Gly Leu Lys Ala Glu Lys Ile Glu Glu Val 
            420                 425                 430         


Val Gln Leu Val Asp Pro Glu Gly Thr His Gly Phe Thr Ala Asp Thr 
        435                 440                 445             


Ile Ala Thr Leu Glu Gly Phe Ala Ala Leu Arg Arg Trp Leu Ser Thr 
    450                 455                 460                 


Gln Glu Thr Ser Tyr Leu Asp Asn Arg Ile Arg Lys Phe Gln Ala Tyr 
465                 470                 475                 480 


Ser Glu Val Gly Asn Gln Pro Leu Pro His Tyr Ala Met Gly Leu Asp 
                485                 490                 495     


Ile Tyr Ala Thr Trp Thr Ser Pro Ile Arg Lys Tyr Gly Asp Met Ile 
            500                 505                 510         


Asn His Arg Met Leu Lys Ala Val Ile Leu Asp Lys Glu Pro Val Gln 
        515                 520                 525             


Lys Pro Asp Asp Gln Val Gly Glu Glu Leu Ala Leu His Arg Lys His 
    530                 535                 540                 


His Lys Ile Ala Glu Arg Asn Val Ser Asp Trp Leu Tyr Ala Arg Thr 
545                 550                 555                 560 


Leu Ala Glu Glu Pro Ser Lys Gln Thr Arg Tyr Ile Gly Glu Ile Phe 
                565                 570                 575     


Asp Ile Asn Arg Ala Gly Ala Arg Val Arg Leu Leu Glu Asn Gly Ala 
            580                 585                 590         


Ala Ala Phe Ile Pro Gly Ser Leu Ile Val Asp Asn Lys Glu Arg Ile 
        595                 600                 605             


Glu Cys Asn Gly Asp Asn Gly Thr Ile Ser Ile Asp Lys Glu Val Val 
    610                 615                 620                 


Tyr Lys Leu Gly Asp Thr Leu Glu Val Val Leu Ala Asp Val Asn Gln 
625                 630                 635                 640 


Glu Asn Arg Ser Leu Val Ala Lys Pro Thr Gln Val Phe Ala Glu Pro 
                645                 650                 655     


Pro Lys Ala Gln Thr Glu Gln Thr Val Glu 
            660                 665     


<210>  27
<211>  505
<212>  PRT
<213>  V. natriegens

<400>  27

Met Thr Glu Gln Lys Tyr Ile Val Ala Leu Asp Gln Gly Thr Thr Ser 
1               5                   10                  15      


Ser Arg Ala Val Ile Leu Asp His Asp Ala Asn Ile Val Ser Val Ala 
            20                  25                  30          


Gln Arg Glu Phe Thr Gln Ile Tyr Pro Gln Ala Gly Trp Val Glu His 
        35                  40                  45              


Asp Pro Met Glu Ile Trp Ala Thr Gln Ser Ser Thr Leu Val Glu Ala 
    50                  55                  60                  


Leu Ala Lys Ser Gly Ile Arg Ser Asp Gln Leu Ala Ala Ile Gly Ile 
65                  70                  75                  80  


Thr Asn Gln Arg Glu Thr Thr Ile Val Trp Asn Lys Glu Thr Gly Lys 
                85                  90                  95      


Pro Val Tyr Asn Ala Ile Val Trp Gln Cys Arg Arg Thr Ala Glu Ile 
            100                 105                 110         


Cys Glu Asp Leu Lys Arg Arg Gly Leu Glu Asp Tyr Val Arg Asp Asn 
        115                 120                 125             


Thr Gly Leu Val Leu Asp Pro Tyr Phe Ser Gly Thr Lys Val Lys Trp 
    130                 135                 140                 


Ile Leu Asp Asn Val Glu Gly Ala Arg Glu Asp Ala Glu Ala Gly Lys 
145                 150                 155                 160 


Leu Leu Phe Gly Thr Val Asp Thr Trp Leu Val Trp Lys Met Thr Gln 
                165                 170                 175     


Gly Arg Val His Val Thr Asp Tyr Thr Asn Ala Ser Arg Thr Met Leu 
            180                 185                 190         


Phe Asn Ile Asn Asp Leu Cys Trp Asp Gln Lys Leu Leu Asp Glu Leu 
        195                 200                 205             


Gly Ile Pro Ala Ser Met Met Pro Glu Val Lys Arg Ser Ser Glu Ile 
    210                 215                 220                 


Tyr Gly Lys Thr Asn Ile Gly Gly Lys Gly Gly Thr Arg Ile Pro Ile 
225                 230                 235                 240 


Ala Gly Ile Ala Gly Asp Gln Gln Ala Ala Leu Tyr Gly Gln Met Cys 
                245                 250                 255     


Val Glu Ala Gly Gln Ala Lys Asn Thr Tyr Gly Thr Gly Cys Phe Leu 
            260                 265                 270         


Leu Met Asn Thr Gly Gln Glu Lys Val Thr Ser Thr His Gly Leu Leu 
        275                 280                 285             


Thr Thr Leu Ala Cys Gly Pro Lys Gly Glu Pro Ala Tyr Ala Leu Glu 
    290                 295                 300                 


Gly Ala Val Phe Met Gly Gly Ala Ser Ile Gln Trp Leu Arg Asp Glu 
305                 310                 315                 320 


Leu Lys Ile Leu Asn Gly Ala Glu Asp Ser Glu Tyr Phe Ala Thr Lys 
                325                 330                 335     


Val Asp Thr Ser Asn Gly Val Tyr Val Val Pro Ala Phe Thr Gly Leu 
            340                 345                 350         


Gly Ala Pro Tyr Trp Asp Ala Tyr Ala Arg Gly Thr Ile Val Gly Leu 
        355                 360                 365             


Thr Arg Gly Val Asn Ser Asn His Ile Ile Arg Ala Thr Leu Glu Gly 
    370                 375                 380                 


Ile Ala Tyr Gln Thr Arg Asp Val Leu Asp Ala Met Gln Ala Asp Ser 
385                 390                 395                 400 


Gly Ile Gln Leu Ala Asn Leu Arg Val Asp Gly Gly Ala Val Ala Asn 
                405                 410                 415     


Asn Phe Leu Met Gln Phe Gln Ser Asp Val Leu Asn Thr Gln Val Leu 
            420                 425                 430         


Arg Pro Glu Val Thr Glu Val Thr Ala Leu Gly Ala Ala Tyr Leu Ala 
        435                 440                 445             


Gly Leu Ala Val Gly Tyr Trp Asp Ser Leu Asp Glu Leu Gln Gly Lys 
    450                 455                 460                 


Ala Val Ile Asp Arg Thr Phe Glu Pro His Asp Asp Glu Glu Lys Arg 
465                 470                 475                 480 


Asn Arg Arg Tyr Lys Gly Trp Lys Arg Ala Val Lys Cys Ala Gln Thr 
                485                 490                 495     


Trp Ser Glu Leu His Asp Glu Asp Asp 
            500                 505 


<210>  28
<211>  451
<212>  PRT
<213>  V. natriegens

<400>  28

Met Ala Thr His Phe Asp Tyr Ile Cys Ile Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Ile Ala Ser Ala Asn Arg Ala Ala Met Tyr Gly Ala Lys Val Ala Leu 
            20                  25                  30          


Ile Glu Ala Gln Asp Leu Gly Gly Thr Cys Val Asn Val Gly Cys Val 
        35                  40                  45              


Pro Lys Lys Val Met Trp His Gly Ala Gln Ile Ala Glu Ala Met Asn 
    50                  55                  60                  


Leu Tyr Ala Glu Asp Tyr Gly Phe Asp Val Asp Val Lys Gly Phe Asp 
65                  70                  75                  80  


Trp Ser Lys Leu Val Glu Ser Arg Gln Ala Tyr Ile Gly Arg Ile His 
                85                  90                  95      


Gln Ser Tyr Asp Arg Val Leu Gly Asn Asn Lys Val Asn Val Ile Lys 
            100                 105                 110         


Gly Phe Ala Lys Phe Val Asp Glu Lys Thr Val Glu Val Asn Gly Glu 
        115                 120                 125             


His Tyr Thr Ala Asp His Ile Leu Ile Ala Val Gly Gly Arg Pro Thr 
    130                 135                 140                 


Ile Pro Asn Ile Pro Gly Ala Glu Tyr Gly Ile Asp Ser Asn Gly Phe 
145                 150                 155                 160 


Phe Asp Leu Ala Glu Gln Pro Lys Arg Val Ala Val Val Gly Ala Gly 
                165                 170                 175     


Tyr Ile Ala Val Glu Ile Ala Gly Val Leu His Ala Leu Gly Thr Glu 
            180                 185                 190         


Thr His Leu Phe Val Arg Lys Glu Ser Pro Leu Arg Ser Phe Asp Pro 
        195                 200                 205             


Met Ile Ile Glu Thr Leu Val Glu Val Met Asp Ala Glu Gly Pro Lys 
    210                 215                 220                 


Leu His Thr His Ser Val Pro Lys Glu Ile Ile Lys Glu Ala Asp Gly 
225                 230                 235                 240 


Thr Leu Thr Leu His Leu Glu Asn Gly Glu Ser Gln Asn Val Asp Gln 
                245                 250                 255     


Leu Ile Trp Ala Ile Gly Arg His Pro Thr Thr Asp Ala Ile Asn Leu 
            260                 265                 270         


Ala Ser Thr Gly Val Ala Thr Asn Asp Lys Gly Tyr Ile Lys Val Asp 
        275                 280                 285             


Glu Tyr Gln Glu Thr Asn Val Lys Gly Ile Tyr Cys Val Gly Asp Ile 
    290                 295                 300                 


Met Glu Gly Gly Ile Glu Leu Thr Pro Val Ala Val Lys Ala Gly Arg 
305                 310                 315                 320 


Gln Leu Ser Glu Arg Leu Phe Asn Asn Lys Pro Asn Ala Lys Met Asp 
                325                 330                 335     


Tyr Asp Leu Val Pro Thr Val Val Phe Ser His Pro Pro Ile Gly Thr 
            340                 345                 350         


Ile Gly Leu Thr Glu Pro Glu Ala Ile Ala Lys Tyr Gly Glu Asp Asn 
        355                 360                 365             


Val Lys Val Tyr Thr Ser Gly Phe Thr Ala Met Tyr Thr Ala Val Thr 
    370                 375                 380                 


Lys His Arg Gln Pro Cys Lys Met Lys Leu Val Cys Ala Gly Glu Glu 
385                 390                 395                 400 


Glu Thr Val Val Gly Leu His Gly Ile Gly Phe Thr Val Asp Glu Met 
                405                 410                 415     


Ile Gln Gly Phe Gly Val Ala Met Lys Met Gly Ala Thr Lys Ala Asp 
            420                 425                 430         


Phe Asp Ser Val Val Ala Ile His Pro Thr Gly Ser Glu Glu Phe Val 
        435                 440                 445             


Thr Met Arg 
    450     


<210>  29
<211>  522
<212>  PRT
<213>  V. natriegens

<400>  29

Met Thr Asp Phe Ala Ala Arg Leu Glu Lys Val Ala Ser Asn Pro Glu 
1               5                   10                  15      


Val Phe Lys Gln Phe Gly Arg Gly Val Glu Arg Glu Thr Leu Arg Tyr 
            20                  25                  30          


Arg Gln Asp Gly Gln Leu Ala Thr Thr Pro His Pro Glu Gly Leu Gly 
        35                  40                  45              


Ser Ala Phe Thr Asn Gln Trp Ile Thr Thr Asp Phe Ser Glu Ser Leu 
    50                  55                  60                  


Leu Glu Phe Ile Thr Pro Val Ser His Asp Val Pro Glu Leu Met Ala 
65                  70                  75                  80  


Gln Leu Lys Asp Ile His His Phe Thr Gln Thr Lys Met Gly Glu Glu 
                85                  90                  95      


Lys Met Trp Pro Leu Ser Met Pro Cys Tyr Val Ala Ser Glu Asp Asn 
            100                 105                 110         


Ile Asn Leu Ala Gln Tyr Gly Ser Ser Asn Ala Ala Arg Met Lys Thr 
        115                 120                 125             


Leu Tyr Arg Glu Gly Leu Lys Arg Arg Tyr Gly Ser Leu Met Gln Ile 
    130                 135                 140                 


Ile Ser Gly Val His Phe Asn Phe Ser Phe Pro Glu Ser Phe Trp Asp 
145                 150                 155                 160 


Ala Leu Tyr Gly Glu Gln Asp Glu Glu Ala Arg Gln Glu Thr Lys Ser 
                165                 170                 175     


Asp Ala Tyr Phe Ala Leu Ile Arg Asn Tyr Tyr Arg Phe Gly Trp Met 
            180                 185                 190         


Ile Pro Tyr Phe Phe Gly Ala Ser Pro Ala Leu Cys Gly Ser Phe Ile 
        195                 200                 205             


Gln Gly Arg Glu Thr Ser Leu Pro Phe Glu Ser Leu Gly Gly Thr Leu 
    210                 215                 220                 


Phe Leu Pro Lys Ser Thr Ser Leu Arg Leu Ser Asp Leu Gly Tyr Thr 
225                 230                 235                 240 


Asn Asn Ala Gln Ser Ser Leu Lys Ile Gly Phe Asn Ser Ile Asp Gln 
                245                 250                 255     


Tyr Leu Glu Gly Leu Ser Asp Ala Ile Arg Arg Pro Ser Glu Glu Phe 
            260                 265                 270         


Ala Lys Ile Gly Val Lys Val Asp Gly Glu Tyr Arg Gln Leu Asn Ser 
        275                 280                 285             


Asn Val Leu Gln Ile Glu Asn Glu Leu Tyr Ala Pro Ile Arg Pro Lys 
    290                 295                 300                 


Arg Val Ala Lys Ser Gly Glu Lys Pro Ser Glu Ala Leu Lys Arg Ala 
305                 310                 315                 320 


Gly Val Glu Tyr Ile Glu Val Arg Ser Leu Asp Val Asn Pro Phe Ser 
                325                 330                 335     


Pro Val Gly Ile Thr Glu Glu Gln Val Arg Phe Leu Asp Leu Phe Leu 
            340                 345                 350         


Thr Trp Ala Ala Leu Ser Asp Ser Glu Pro Met Asp Asn Cys Glu Leu 
        355                 360                 365             


Glu Cys Trp Arg Asp Asn Trp Asn Lys Val Ile Val Ser Gly Arg Glu 
    370                 375                 380                 


Lys Gly Leu Met Leu Gln Ile Gly Cys Gln Gly Glu Arg Leu Pro Leu 
385                 390                 395                 400 


Gln Glu Trp Ala His Arg Val Phe Ala Asp Leu Arg Gln Ile Ala Val 
                405                 410                 415     


Met Met Asp Glu Val Asn Gly Asp Asn Ala Tyr Gln Glu Val Cys Asp 
            420                 425                 430         


Lys Leu Thr Gly Trp Ile Asp Glu Pro Glu Leu Thr Thr Ser Gly Gln 
        435                 440                 445             


Leu Leu Glu Leu Thr Lys Glu Leu Gly Gly Leu Gly Lys Val Gly Cys 
    450                 455                 460                 


Ser Leu Gly Met Lys His Arg Glu Asp Asn Leu Asn His Gly Tyr Gln 
465                 470                 475                 480 


His Tyr Ser Gln Glu Val Met Glu Gln Glu Ala Leu Ala Ser Val Glu 
                485                 490                 495     


Lys Gln Lys Gln Ala Glu Leu Ser Asp Thr Met Ser Phe Asp Asp Phe 
            500                 505                 510         


Leu Glu Asp Tyr Phe Ser Tyr Leu Lys Gln 
        515                 520         


<210>  30
<211>  486
<212>  PRT
<213>  V. natriegens

<400>  30

Met Glu Asn Phe Lys His Leu Pro Glu Pro Phe Arg Ile Arg Val Ile 
1               5                   10                  15      


Glu Pro Val Lys Arg Thr Thr Arg Glu Tyr Arg Glu Glu Ala Ile Leu 
            20                  25                  30          


Lys Ala Gly Met Asn Pro Phe Leu Leu Asp Ser Glu Asp Val Phe Ile 
        35                  40                  45              


Asp Leu Leu Thr Asp Ser Gly Thr Gly Ala Ile Thr Gln Glu Met Gln 
    50                  55                  60                  


Ala Ala Met Leu Arg Gly Asp Glu Ala Tyr Ser Gly Ser Arg Ser Tyr 
65                  70                  75                  80  


His Ala Leu Gln Gln Ala Val Glu Asp Ile Phe Gly Tyr Gln Tyr Thr 
                85                  90                  95      


Ile Pro Thr His Gln Gly Arg Gly Ala Glu Gln Ile Tyr Ile Pro Val 
            100                 105                 110         


Leu Ile Lys Lys Arg Glu Lys Glu Lys Glu Leu Asp Arg Ser Lys Met 
        115                 120                 125             


Val Ala Leu Ser Asn Tyr Phe Phe Asp Thr Thr Gln Gly His Thr Gln 
    130                 135                 140                 


Leu Asn Cys Cys Val Ala Lys Asn Val Tyr Thr Glu Glu Ala Phe Asp 
145                 150                 155                 160 


Thr Ser Ile Glu Ala Asp Phe Lys Gly Asn Phe Asp Leu Glu Lys Leu 
                165                 170                 175     


Glu Gln Ala Ile Leu Glu Ala Gly Ala Ala Asn Val Pro Tyr Ile Val 
            180                 185                 190         


Ser Thr Ile Thr Cys Asn Ser Ala Gly Gly Gln Pro Val Ser Leu Ala 
        195                 200                 205             


Asn Leu Lys Ala Val Tyr Glu Ile Gly Gln Lys Tyr Asp Ile Pro Val 
    210                 215                 220                 


Ile Met Asp Ser Ala Arg Phe Ala Glu Asn Ala Tyr Phe Ile Gln Gln 
225                 230                 235                 240 


Arg Glu Ala Gly Tyr Ala Asp Trp Ser Ile Glu Ala Ile Thr Lys Glu 
                245                 250                 255     


Ser Tyr Lys Tyr Ala Asp Gly Leu Ala Met Ser Ala Lys Lys Asp Ala 
            260                 265                 270         


Met Val Gln Met Gly Gly Leu Leu Cys Phe Lys Asp Asp Ser Leu Met 
        275                 280                 285             


Asp Val Tyr Thr Glu Cys Arg Thr Leu Cys Val Val Gln Glu Gly Phe 
    290                 295                 300                 


Pro Thr Tyr Gly Gly Leu Glu Gly Gly Ala Met Glu Arg Leu Ala Val 
305                 310                 315                 320 


Gly Leu Tyr Asp Gly Met Arg Gln Asp Trp Leu Ala Tyr Arg Ile Gly 
                325                 330                 335     


Gln Val Gln Tyr Leu Val Asp Lys Leu Glu Ala Ile Gly Ile Val Cys 
            340                 345                 350         


Gln Gln Ala Gly Gly His Ala Ala Phe Val Asp Ala Ser Lys Leu Leu 
        355                 360                 365             


Pro His Ile Pro Ala Glu Gln Phe Pro Ala His Ala Leu Ala Cys Glu 
    370                 375                 380                 


Leu Tyr Lys Val Ala Gly Ile Arg Ala Val Glu Ile Gly Ser Leu Leu 
385                 390                 395                 400 


Leu Gly Arg Asp Pro Ala Thr Gly Lys Gln His Pro Cys Pro Ala Glu 
                405                 410                 415     


Leu Leu Arg Leu Thr Ile Pro Arg Ala Thr Tyr Thr Gln Thr His Met 
            420                 425                 430         


Asp Phe Ile Val Glu Ala Phe Gln Ala Val Lys Glu Asn Ala Ala Asn 
        435                 440                 445             


Val Lys Gly Leu Asp Phe Thr Tyr Glu Pro Glu Val Leu Arg His Phe 
    450                 455                 460                 


Thr Ala Arg Leu Lys Glu Val Glu Ser Glu Thr Gln Ala Pro Ser Ile 
465                 470                 475                 480 


Thr Thr Ala Glu Thr Val 
                485     


