                         SEQUENCE LISTING

<110>  UNIVERSITY OF DELHI SOUTH CAMPUS
 
<120>  A PROCESS FOR IMMOBILIZING POLYPEPTIDES

<130>  PD027163IN-SC

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 1 depicts recombinant MTC28 amino acid sequence

<400>  1

Gly Ala Ser Gly Ser Asp Pro Leu Leu Pro Pro Pro Pro Ile Pro Ala 
1               5                   10                  15      


Pro Val Ser Ala Pro Ala Thr Val Pro Pro Val Gln Asn Leu Thr Ala 
            20                  25                  30          


Leu Pro Gly Gly Ser Ser Asn Arg Phe Ser Pro Ala Pro Ala Pro Ala 
        35                  40                  45              


Pro Ile Ala Ser Pro Ile Pro Val Gly Ala Pro Gly Ser Thr Ala Val 
    50                  55                  60                  


Pro Pro Leu Pro Pro Pro Val Thr Pro Ala Ile Ser Gly Thr Leu Arg 
65                  70                  75                  80  


Asp His Leu Arg Glu Lys Gly Val Lys Leu Glu Ala Gln Arg Pro His 
                85                  90                  95      


Gly Phe Lys Ala Leu Asp Ile Thr Leu Pro Met Pro Pro Arg Trp Thr 
            100                 105                 110         


Gln Val Pro Asp Pro Asn Val Pro Asp Ala Phe Val Val Ile Ala Asp 
        115                 120                 125             


Arg Leu Gly Asn Ser Val Tyr Thr Ser Asn Ala Gln Leu Val Val Tyr 
    130                 135                 140                 


Arg Leu Ile Gly Asp Phe Asp Pro Ala Glu Ala Ile Thr His Gly Tyr 
145                 150                 155                 160 


Ile Asp Ser Gln Lys Leu Leu Ala Trp Gln Thr Thr Asn Ala Ser Met 
                165                 170                 175     


Ala Asn Phe Asp Gly Phe Pro Ser Ser Ile Ile Glu Gly Thr Tyr Arg 
            180                 185                 190         


Glu Asn Asp Met Thr Leu Asn Thr Ser Arg Arg His Val Ile Ala Thr 
        195                 200                 205             


Ser Gly Ala Asp Lys Tyr Leu Val Ser Leu Ser Val Thr Thr Ala Leu 
    210                 215                 220                 


Ser Gln Ala Val Thr Asp Gly Pro Ala Thr Asp Ala Ile Val Asn Gly 
225                 230                 235                 240 


Phe Gln Val Val Ala His Ala Ala Pro Ala Gln Ala Pro Ala Pro Ala 
                245                 250                 255     


Pro Gly Ser Ala Pro Val Gly Leu Pro Gly Gln Ala Pro Gly Tyr Pro 
            260                 265                 270         


Pro Ala Gly Thr Leu Thr Pro Val Pro Pro Arg Gly Gly Gly Ala Ser 
        275                 280                 285             


Gly Gly Ala Pro Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile 
    290                 295                 300                 


Glu Trp His Glu 
305             


<210>  2
<211>  924
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 2 depicts recombinant MTC28 nucelic acid sequence

<400>  2
ggtgctagcg gcagcgatcc cctgctgcca ccgccgccta tccctgcccc agtctcggcg       60

ccggcaacag tcccgcccgt gcagaacctc acggcgcttc cgggcgggag cagcaacagg      120

ttctcaccgg cgccagcacc cgcaccgatc gcgtcgccga ttccggtcgg agcacccggg      180

tccaccgctg tgcccccgct gccgccgcca gtgactcccg cgatcagcgg cacacttcgg      240

gaccacctcc gggagaaggg cgtcaagctg gaggcacagc gaccgcacgg attcaaggcg      300

ctcgacatca cactgcccat gccgccgcgc tggactcagg tgcccgaccc caacgtgccc      360

gacgcgttcg tggtgatcgc cgaccggttg ggcaacagcg tctacacgtc gaatgcgcag      420

ctggtagtgt ataggctgat cggtgacttc gatcccgctg aggccatcac acacggctac      480

attgacagcc agaaattgct cgcatggcag accacaaacg cctcgatggc caatttcgac      540

ggctttccgt catcaatcat cgagggcacc taccgcgaaa acgacatgac cctcaacacc      600

tcccggcgcc acgtcatcgc cacctccgga gccgacaagt acctggtttc gctgtcggtg      660

accaccgcgc tgtcgcaggc ggtcaccgac gggccggcca ccgatgcgat tgtcaacgga      720

ttccaagtgg ttgcgcatgc ggcgcccgct caggcgcctg ccccggcacc cggttcggca      780

ccggtgggac tacccgggca ggcgcctggg tatccgcccg cgggcaccct gacaccagtc      840

ccgccgcgcg gtggaggcgc ctcaggcggc gcgcctggag gtctgaacga catcttcgag      900

gctcagaaaa tcgaatggca cgag                                             924


<210>  3
<211>  160
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 3 depicts recombinant MPT63 amino acid sequence

<400>  3

Gly Ala Ser Gly Ser Ala Tyr Pro Ile Thr Gly Lys Leu Gly Ser Glu 
1               5                   10                  15      


Leu Thr Met Thr Asp Thr Val Gly Gln Val Val Leu Gly Trp Lys Val 
            20                  25                  30          


Ser Asp Leu Lys Ser Ser Thr Ala Val Ile Pro Gly Tyr Pro Val Ala 
        35                  40                  45              


Gly Gln Val Trp Glu Ala Thr Ala Thr Val Asn Ala Ile Arg Gly Ser 
    50                  55                  60                  


Val Thr Pro Ala Val Ser Gln Phe Asn Ala Arg Thr Ala Asp Gly Ile 
65                  70                  75                  80  


Asn Tyr Arg Val Leu Trp Gln Ala Ala Gly Pro Asp Thr Ile Ser Gly 
                85                  90                  95      


Ala Thr Ile Pro Gln Gly Glu Gln Ser Thr Gly Lys Ile Tyr Phe Asp 
            100                 105                 110         


Val Thr Gly Pro Ser Pro Thr Ile Val Ala Met Asn Asn Gly Met Glu 
        115                 120                 125             


Asp Leu Leu Ile Trp Glu Pro Gly Gly Gly Ala Ser Gly Gly Ala Pro 
    130                 135                 140                 


Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
145                 150                 155                 160 


<210>  4
<211>  480
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 4 depicts recombinant MPT63 nucleotide sequence

<400>  4
ggtgctagcg gcagcgccta tcccatcacc ggaaaacttg gcagtgagct aacgatgacc       60

gacaccgttg gccaagtcgt gctcggctgg aaggtcagtg atctcaaatc cagcacggca      120

gtcatccccg gctatccggt ggccggccag gtctgggagg ccactgccac ggtcaatgcg      180

attcgcggca gcgtcacgcc cgcggtctcg cagttcaatg cccgcaccgc cgacggcatc      240

aactaccggg tgctgtggca agccgcgggc cccgacacca ttagcggagc cactatcccc      300

caaggcgaac aatcgaccgg caaaatctac ttcgatgtca ccggcccatc gccaaccatc      360

gtcgcgatga acaacggcat ggaggatctg ctgatttggg agccgggtgg aggcgcctca      420

ggcggcgcgc ctggaggtct gaacgacatc ttcgaggctc agaaaatcga atggcacgag      480


<210>  5
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 5 depicts recombinant MPT64 amino acid sequence

<400>  5

Gly Ala Ser Gly Ser Ala Pro Lys Thr Tyr Cys Glu Glu Leu Lys Gly 
1               5                   10                  15      


Thr Asp Thr Gly Gln Ala Cys Gln Ile Gln Met Ser Asp Pro Ala Tyr 
            20                  25                  30          


Asn Ile Asn Ile Ser Leu Pro Ser Tyr Tyr Pro Asp Gln Lys Ser Leu 
        35                  40                  45              


Glu Asn Tyr Ile Ala Gln Thr Arg Asp Lys Phe Leu Ser Ala Ala Thr 
    50                  55                  60                  


Ser Ser Thr Pro Arg Glu Ala Pro Tyr Glu Leu Asn Ile Thr Ser Ala 
65                  70                  75                  80  


Thr Tyr Gln Ser Ala Ile Pro Pro Arg Gly Thr Gln Ala Val Val Leu 
                85                  90                  95      


Lys Val Tyr Gln Asn Ala Gly Gly Thr His Pro Thr Thr Thr Tyr Lys 
            100                 105                 110         


Ala Phe Asp Trp Asp Gln Ala Tyr Arg Lys Pro Ile Thr Tyr Asp Thr 
        115                 120                 125             


Leu Trp Gln Ala Asp Thr Asp Pro Leu Pro Val Val Phe Pro Ile Val 
    130                 135                 140                 


Gln Gly Glu Leu Ser Lys Gln Thr Gly Gln Gln Val Ser Ile Ala Pro 
145                 150                 155                 160 


Asn Ala Gly Leu Asp Pro Val Asn Tyr Gln Asn Phe Ala Val Thr Asn 
                165                 170                 175     


Asp Gly Val Ile Phe Phe Phe Asn Pro Gly Glu Leu Leu Pro Glu Ala 
            180                 185                 190         


Ala Gly Pro Thr Gln Val Leu Val Pro Arg Ser Ala Ile Asp Ser Met 
        195                 200                 205             


Leu Ala Gly Gly Gly Ala Ser Gly Gly Ala Pro Gly Gly Leu Asn Asp 
    210                 215                 220                 


Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
225                 230                 235 


<210>  6
<211>  705
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 6 depicts recombinant MPT64 nucleotide sequence

<400>  6
ggtgctagcg gcagcgcgcc caagacctac tgcgaggagt tgaaaggcac cgataccggc       60

caggcgtgcc agattcaaat gtccgacccg gcctacaaca tcaacatcag cctgcccagt      120

tactaccccg accagaagtc gctggaaaat tacatcgccc agacgcgcga caagttcctc      180

agcgcggcca catcgtccac tccacgcgaa gccccctacg aattgaatat cacctcggcc      240

acataccagt ccgcgatacc gccgcgtggt acgcaggccg tggtgctcaa ggtctaccag      300

aacgccggcg gcacgcaccc aacgaccacg tacaaggcct tcgattggga ccaggcctat      360

cgcaagccaa tcacctatga cacgctgtgg caggctgaca ccgatccgct gccagtcgtc      420

ttccccattg tgcaaggtga actgagcaag cagaccggac aacaggtatc gatagcgccg      480

aatgccggct tggacccggt gaattatcag aacttcgcag tcacgaacga cggggtgatt      540

ttcttcttca acccggggga gttgctgccc gaagcagccg gcccaaccca ggtattggtc      600

ccacgttccg cgatcgactc gatgctggcc ggtggaggcg cctcaggcgg cgcgcctgga      660

ggtctgaacg acatcttcga ggctcagaaa atcgaatggc acgag                      705


<210>  7
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 7 depicts recombinant Ag85A amino acid sequence

<400>  7

Gly Ala Ser Gly Ser Phe Ser Arg Pro Gly Leu Pro Val Glu Tyr Leu 
1               5                   10                  15      


Gln Val Pro Ser Pro Ser Met Gly Arg Asp Ile Lys Val Gln Phe Gln 
            20                  25                  30          


Ser Gly Gly Ala Asn Ser Pro Ala Leu Tyr Leu Leu Asp Gly Leu Arg 
        35                  40                  45              


Ala Gln Asp Asp Phe Ser Gly Trp Asp Ile Asn Thr Pro Ala Phe Glu 
    50                  55                  60                  


Trp Tyr Asp Gln Ser Gly Leu Ser Val Val Met Pro Val Gly Gly Gln 
65                  70                  75                  80  


Ser Ser Phe Tyr Ser Asp Trp Tyr Gln Pro Ala Cys Gly Lys Ala Gly 
                85                  90                  95      


Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu Thr Ser Glu Leu Pro Gly 
            100                 105                 110         


Trp Leu Gln Ala Asn Arg His Val Lys Pro Thr Gly Ser Ala Val Val 
        115                 120                 125             


Gly Leu Ser Met Ala Ala Ser Ser Ala Leu Thr Leu Ala Ile Tyr His 
    130                 135                 140                 


Pro Gln Gln Phe Val Tyr Ala Gly Ala Met Ser Gly Leu Leu Asp Pro 
145                 150                 155                 160 


Ser Gln Ala Met Gly Pro Thr Leu Ile Gly Leu Ala Met Gly Asp Ala 
                165                 170                 175     


Gly Gly Tyr Lys Ala Ser Asp Met Trp Gly Pro Lys Glu Asp Pro Ala 
            180                 185                 190         


Trp Gln Arg Asn Asp Pro Leu Leu Asn Val Gly Lys Leu Ile Ala Asn 
        195                 200                 205             


Asn Thr Arg Val Trp Val Tyr Cys Gly Asn Gly Lys Pro Ser Asp Leu 
    210                 215                 220                 


Gly Gly Asn Asn Leu Pro Ala Lys Phe Leu Glu Gly Phe Val Arg Thr 
225                 230                 235                 240 


Ser Asn Ile Lys Phe Gln Asp Ala Tyr Asn Ala Gly Gly Gly His Asn 
                245                 250                 255     


Gly Val Phe Asp Phe Pro Asp Ser Gly Thr His Ser Trp Glu Tyr Trp 
            260                 265                 270         


Gly Ala Gln Leu Asn Ala Met Lys Pro Asp Leu Gln Arg Ala Leu Gly 
        275                 280                 285             


Ala Thr Pro Asn Thr Gly Pro Ala Pro Gln Gly Ala Gly Gly Gly Ala 
    290                 295                 300                 


Ser Gly Gly Ala Pro Gly Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys 
305                 310                 315                 320 


Ile Glu Trp His Glu 
                325 


<210>  8
<211>  975
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 8 depicts recombinant Ag85A nucleotide sequence

<400>  8
ggtgctagcg gcagcttttc ccggccgggc ttgccggtgg agtacctgca ggtgccgtcg       60

ccgtcgatgg gccgtgacat caaggtccaa ttccaaagtg gtggtgccaa ctcgcccgcc      120

ctgtacctgc tcgacggcct gcgcgcgcag gacgacttca gcggctggga catcaacacc      180

ccggcgttcg agtggtacga ccagtcgggc ctgtcggtgg tcatgccggt gggtggccag      240

tcaagcttct actccgactg gtaccagccc gcctgcggca aggccggttg ccagacttac      300

aagtgggaga ccttcctgac cagcgagctg ccggggtggc tgcaggccaa caggcacgtc      360

aagcccaccg gaagcgccgt cgtcggtctt tcgatggctg cttcttcggc gctgacgctg      420

gcgatctatc acccccagca gttcgtctac gcgggagcga tgtcgggcct gttggacccc      480

tcccaggcga tgggtcccac cctgatcggc ctggcgatgg gtgacgctgg cggctacaag      540

gcctccgaca tgtggggccc gaaggaggac ccggcgtggc agcgcaacga cccgctgttg      600

aacgtcggga agctgatcgc caacaacacc cgcgtctggg tgtactgcgg caacggcaag      660

ccgtcggatc tgggtggcaa caacctgccg gccaagttcc tcgagggctt cgtgcggacc      720

agcaacatca agttccaaga cgcctacaac gccggtggcg gccacaacgg cgtgttcgac      780

ttcccggaca gcggtacgca cagctgggag tactggggcg cgcagctcaa cgctatgaag      840

cccgacctgc aacgggcact gggtgccacg cccaacaccg ggcccgcgcc ccagggcgcc      900

ggtggaggcg cctcaggcgg cgcgcctgga ggtctgaacg acatcttcga ggctcagaaa      960

atcgaatggc acgag                                                       975


<210>  9
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 9 depicts recombinant Ag85B amino acid sequence

<400>  9

Gly Ala Ser Gly Ser Phe Ser Arg Pro Gly Leu Pro Val Glu Tyr Leu 
1               5                   10                  15      


Gln Val Pro Ser Pro Ser Met Gly Arg Asp Ile Lys Val Gln Phe Gln 
            20                  25                  30          


Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr Leu Leu Asp Gly Leu Arg 
        35                  40                  45              


Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile Asn Thr Pro Ala Phe Glu 
    50                  55                  60                  


Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val Met Pro Val Gly Gly Gln 
65                  70                  75                  80  


Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro Ala Cys Gly Lys Ala Gly 
                85                  90                  95      


Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu Thr Ser Glu Leu Pro Gln 
            100                 105                 110         


Trp Leu Ser Ala Asn Arg Ala Val Lys Pro Thr Gly Ser Ala Ala Ile 
        115                 120                 125             


Gly Leu Ser Met Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His 
    130                 135                 140                 


Pro Gln Gln Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro 
145                 150                 155                 160 


Ser Gln Gly Met Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala 
                165                 170                 175     


Gly Gly Tyr Lys Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala 
            180                 185                 190         


Trp Glu Arg Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn 
        195                 200                 205             


Asn Thr Arg Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu 
    210                 215                 220                 


Gly Gly Ala Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser 
225                 230                 235                 240 


Ser Asn Leu Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn 
                245                 250                 255     


Ala Val Phe Asn Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp 
            260                 265                 270         


Gly Ala Gln Leu Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly 
        275                 280                 285             


Ala Gly Gly Gly Gly Ala Ser Gly Gly Ala Pro Gly Gly Leu Asn Asp 
    290                 295                 300                 


Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
305                 310                 315 


<210>  10
<211>  945
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 10 depicts recombinant Ag85B nucleotide sequence

<400>  10
ggtgctagcg gcagcttctc ccggccgggg ctgccggtcg agtacctgca ggtgccgtcg       60

ccgtcgatgg gccgcgacat caaggttcag ttccagagcg gtgggaacaa ctcacctgcg      120

gtttatctgc tcgacggcct gcgcgcccaa gacgactaca acggctggga tatcaacacc      180

ccggcgttcg agtggtacta ccagtcggga ctgtcgatag tcatgccggt cggcgggcag      240

tccagcttct acagcgactg gtacagcccg gcctgcggta aggctggctg ccagacttac      300

aagtgggaaa ccttcctgac cagcgagctg ccgcaatggt tgtccgccaa cagggccgtg      360

aagcccaccg gcagcgctgc aatcggcttg tcgatggccg gctcgtcggc aatgatcttg      420

gccgcctacc acccccagca gttcatctac gccggctcgc tgtcggccct gctggacccc      480

tctcagggga tggggcctag cctgatcggc ctcgcgatgg gtgacgccgg cggttacaag      540

gccgcagaca tgtggggtcc ctcgagtgac ccggcatggg agcgcaacga ccctacgcag      600

cagatcccca agctggtcgc aaacaacacc cggctatggg tttattgcgg gaacggcacc      660

ccgaacgagt tgggcggtgc caacataccc gccgagttct tggagaactt cgttcgtagc      720

agcaacctga agttccagga tgcgtacaac gccgcgggcg ggcacaacgc cgtgttcaac      780

ttcccgccca acggcacgca cagctgggag tactggggcg ctcagctcaa cgccatgaag      840

ggtgacctgc agagttcgtt aggcgccggc ggtggaggcg cctcaggcgg cgcgcctgga      900

ggtctgaacg acatcttcga ggctcagaaa atcgaatggc acgag                      945


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 11 depicts BAP tag amino acid sequence

<400>  11

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  12
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 12 depicts the primer sequence Ag85A-5-1

<400>  12
cggcagcttt tcccggccgg gcttgccggt g                                      31


<210>  13
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 13 depicts the primer sequence Ag85A-N3-1

<400>  13
ctccaccggc gccctggggc gcgggcccgg t                                      31


<210>  14
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 14 depicts the primer sequence Ag85B-5-1

<400>  14
cggcagcttc tcccggccgg ggctgccggt c                                      31


<210>  15
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 15 depicts the primr sequence Ag85B-N3-1

<400>  15
ctccaccgcc ggcgcctaac gaactctgca g                                      31


<210>  16
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 16 depicts the primer sequence MPT63-5-1

<400>  16
cggcagcgcc tatcccatca ccggaaaact t                                      31


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 17 depicts the primer sequence MPT63-N3-1

<400>  17
ctccacccgg ctcccaaatc agcagatcct c                                      31


<210>  18
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 18 depicts the primer sequence MPT64-5-1

<400>  18
cggcagcgcg cccaagacct actgcgagga g                                      31


<210>  19
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 19 depicts the primer sequence MPT64-N3-1

<400>  19
ctccaccggc cagcatcgag tcgatcgcgg a                                      31


<210>  20
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 20 depicts the primer sequence MTC28-51

<400>  20
cggcagcgat cccctgctgc caccgccgcc tatc                                   34


<210>  21
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEQ ID NO: 21 depicts the primer sequence MTC28-31

<400>  21
ctccaccgcg cggcgggact ggtgtcaggg t                                      31


