                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  CELL

<130>  P113363PCT

<150>  GB 1718697.4
<151>  2017-11-13

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  418
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence for VioA

<400>  1

Met Lys His Ser Ser Asp Ile Cys Ile Val Gly Ala Gly Ile Ser Gly 
1               5                   10                  15      


Leu Thr Cys Ala Ser His Leu Leu Asp Ser Pro Ala Cys Arg Gly Leu 
            20                  25                  30          


Ser Leu Arg Ile Phe Asp Met Gln Gln Glu Ala Gly Gly Arg Ile Arg 
        35                  40                  45              


Ser Lys Met Leu Asp Gly Lys Ala Ser Ile Glu Leu Gly Ala Gly Arg 
    50                  55                  60                  


Tyr Ser Pro Gln Leu His Pro His Phe Gln Ser Ala Met Gln His Tyr 
65                  70                  75                  80  


Ser Gln Lys Ser Glu Val Tyr Pro Phe Thr Gln Leu Lys Phe Lys Ser 
                85                  90                  95      


His Val Gln Gln Lys Leu Lys Arg Ala Met Asn Glu Leu Ser Pro Arg 
            100                 105                 110         


Leu Lys Glu His Gly Lys Glu Ser Phe Leu Gln Phe Val Ser Arg Tyr 
        115                 120                 125             


Gln Gly His Asp Ser Ala Val Gly Met Ile Arg Ser Met Gly Tyr Asp 
    130                 135                 140                 


Ala Leu Phe Leu Pro Asp Ile Ser Ala Glu Met Ala Tyr Asp Ile Val 
145                 150                 155                 160 


Gly Lys His Pro Glu Ile Gln Ser Val Thr Asp Asn Asp Ala Asn Gln 
                165                 170                 175     


Trp Phe Ala Ala Glu Thr Gly Phe Ala Gly Leu Ile Gln Gly Ile Lys 
            180                 185                 190         


Ala Lys Val Lys Ala Ala Gly Ala Arg Phe Ser Leu Gly Tyr Arg Leu 
        195                 200                 205             


Leu Ser Val Arg Thr Asp Gly Asp Gly Tyr Leu Leu Gln Leu Ala Gly 
    210                 215                 220                 


Asp Asp Gly Trp Lys Leu Glu His Arg Thr Arg His Leu Ile Leu Ala 
225                 230                 235                 240 


Ile Pro Pro Ser Ala Met Ala Gly Leu Asn Val Asp Phe Pro Glu Ala 
                245                 250                 255     


Trp Ser Gly Ala Arg Tyr Gly Ser Leu Pro Leu Phe Lys Gly Phe Leu 
            260                 265                 270         


Thr Tyr Gly Glu Pro Trp Trp Leu Asp Tyr Lys Leu Asp Asp Gln Val 
        275                 280                 285             


Leu Ile Val Asp Asn Pro Leu Arg Lys Ile Tyr Phe Lys Gly Asp Lys 
    290                 295                 300                 


Tyr Leu Phe Phe Tyr Thr Asp Ser Glu Met Ala Asn Tyr Trp Arg Gly 
305                 310                 315                 320 


Cys Val Ala Glu Gly Glu Asp Gly Tyr Leu Glu Gln Ile Arg Thr His 
                325                 330                 335     


Leu Ala Ser Ala Leu Gly Ile Val Arg Glu Arg Ile Pro Gln Pro Leu 
            340                 345                 350         


Ala His Val His Lys Tyr Trp Ala His Gly Val Glu Phe Cys Arg Asp 
        355                 360                 365             


Ser Asp Ile Asp His Pro Ser Ala Leu Ser His Arg Asp Ser Gly Ile 
    370                 375                 380                 


Ile Ala Cys Ser Asp Ala Tyr Thr Glu His Cys Gly Trp Met Glu Gly 
385                 390                 395                 400 


Gly Leu Leu Ser Ala Arg Glu Ala Ser Arg Leu Leu Leu Gln Arg Ile 
                405                 410                 415     


Ala Ala 
        


<210>  2
<211>  998
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence for VioB

<400>  2

Met Ser Ile Leu Asp Phe Pro Arg Ile His Phe Arg Gly Trp Ala Arg 
1               5                   10                  15      


Val Asn Ala Pro Thr Ala Asn Arg Asp Pro His Gly His Ile Asp Met 
            20                  25                  30          


Ala Ser Asn Thr Val Ala Met Ala Gly Glu Pro Phe Asp Leu Ala Arg 
        35                  40                  45              


His Pro Thr Glu Phe His Arg His Leu Arg Ser Leu Gly Pro Arg Phe 
    50                  55                  60                  


Gly Leu Asp Gly Arg Ala Asp Pro Glu Gly Pro Phe Ser Leu Ala Glu 
65                  70                  75                  80  


Gly Tyr Asn Ala Ala Gly Asn Asn His Phe Ser Trp Glu Ser Ala Thr 
                85                  90                  95      


Val Ser His Val Gln Trp Asp Gly Gly Glu Ala Asp Arg Gly Asp Gly 
            100                 105                 110         


Leu Val Gly Ala Arg Leu Ala Leu Trp Gly His Tyr Asn Asp Tyr Leu 
        115                 120                 125             


Arg Thr Thr Phe Asn Arg Ala Arg Trp Val Asp Ser Asp Pro Thr Arg 
    130                 135                 140                 


Arg Asp Ala Ala Gln Ile Tyr Ala Gly Gln Phe Thr Ile Ser Pro Ala 
145                 150                 155                 160 


Gly Ala Gly Pro Gly Thr Pro Trp Leu Phe Thr Ala Asp Ile Asp Asp 
                165                 170                 175     


Ser His Gly Ala Arg Trp Thr Arg Gly Gly His Ile Ala Glu Arg Gly 
            180                 185                 190         


Gly His Phe Leu Asp Glu Glu Phe Gly Leu Ala Arg Leu Phe Gln Phe 
        195                 200                 205             


Ser Val Pro Lys Asp His Pro His Phe Leu Phe His Pro Gly Pro Phe 
    210                 215                 220                 


Asp Ser Glu Ala Trp Arg Arg Leu Gln Leu Ala Leu Glu Asp Asp Asp 
225                 230                 235                 240 


Val Leu Gly Leu Thr Val Gln Tyr Ala Leu Phe Asn Met Ser Thr Pro 
                245                 250                 255     


Pro Gln Pro Asn Ser Pro Val Phe His Asp Met Val Gly Val Val Gly 
            260                 265                 270         


Leu Trp Arg Arg Gly Glu Leu Ala Ser Tyr Pro Ala Gly Arg Leu Leu 
        275                 280                 285             


Arg Pro Arg Gln Pro Gly Leu Gly Asp Leu Thr Leu Arg Val Asn Gly 
    290                 295                 300                 


Gly Arg Val Ala Leu Asn Leu Ala Cys Ala Ile Pro Phe Ser Thr Arg 
305                 310                 315                 320 


Ala Ala Gln Pro Ser Ala Pro Asp Arg Leu Thr Pro Asp Leu Gly Ala 
                325                 330                 335     


Lys Leu Pro Leu Gly Asp Leu Leu Leu Arg Asp Glu Asp Gly Ala Leu 
            340                 345                 350         


Leu Ala Arg Val Pro Gln Ala Leu Tyr Gln Asp Tyr Trp Thr Asn His 
        355                 360                 365             


Gly Ile Val Asp Leu Pro Leu Leu Arg Glu Pro Arg Gly Ser Leu Thr 
    370                 375                 380                 


Leu Ser Ser Glu Leu Ala Glu Trp Arg Glu Gln Asp Trp Val Thr Gln 
385                 390                 395                 400 


Ser Asp Ala Ser Asn Leu Tyr Leu Glu Ala Pro Asp Arg Arg His Gly 
                405                 410                 415     


Arg Phe Phe Pro Glu Ser Ile Ala Leu Arg Ser Tyr Phe Arg Gly Glu 
            420                 425                 430         


Ala Arg Ala Arg Pro Asp Ile Pro His Arg Ile Glu Gly Met Gly Leu 
        435                 440                 445             


Val Gly Val Glu Ser Arg Gln Asp Gly Asp Ala Ala Glu Trp Arg Leu 
    450                 455                 460                 


Thr Gly Leu Arg Pro Gly Pro Ala Arg Ile Val Leu Asp Asp Gly Ala 
465                 470                 475                 480 


Glu Ala Ile Pro Leu Arg Val Leu Pro Asp Asp Trp Ala Leu Asp Asp 
                485                 490                 495     


Ala Thr Val Glu Glu Val Asp Tyr Ala Phe Leu Tyr Arg His Val Met 
            500                 505                 510         


Ala Tyr Tyr Glu Leu Val Tyr Pro Phe Met Ser Asp Lys Val Phe Ser 
        515                 520                 525             


Leu Ala Asp Arg Cys Lys Cys Glu Thr Tyr Ala Arg Leu Met Trp Gln 
    530                 535                 540                 


Met Cys Asp Pro Gln Asn Arg Asn Lys Ser Tyr Tyr Met Pro Ser Thr 
545                 550                 555                 560 


Arg Glu Leu Ser Ala Pro Lys Ala Arg Leu Phe Leu Lys Tyr Leu Ala 
                565                 570                 575     


His Val Glu Gly Gln Ala Arg Leu Gln Ala Pro Pro Pro Ala Gly Pro 
            580                 585                 590         


Ala Arg Ile Glu Ser Lys Ala Gln Leu Ala Ala Glu Leu Arg Lys Ala 
        595                 600                 605             


Val Asp Leu Glu Leu Ser Val Met Leu Gln Tyr Leu Tyr Ala Ala Tyr 
    610                 615                 620                 


Ser Ile Pro Asn Tyr Ala Gln Gly Gln Gln Arg Val Arg Asp Gly Ala 
625                 630                 635                 640 


Trp Thr Ala Glu Gln Leu Gln Leu Ala Cys Gly Ser Gly Asp Arg Arg 
                645                 650                 655     


Arg Asp Gly Gly Ile Arg Ala Ala Leu Leu Glu Ile Ala His Glu Glu 
            660                 665                 670         


Met Ile His Tyr Leu Val Val Asn Asn Leu Leu Met Ala Leu Gly Glu 
        675                 680                 685             


Pro Phe Tyr Ala Gly Val Pro Leu Met Gly Glu Ala Ala Arg Gln Ala 
    690                 695                 700                 


Phe Gly Leu Asp Thr Glu Phe Ala Leu Glu Pro Phe Ser Glu Ser Thr 
705                 710                 715                 720 


Leu Ala Arg Phe Val Arg Leu Glu Trp Pro His Phe Ile Pro Ala Pro 
                725                 730                 735     


Gly Lys Ser Ile Ala Asp Cys Tyr Ala Ala Ile Arg Gln Ala Phe Leu 
            740                 745                 750         


Asp Leu Pro Asp Leu Phe Gly Gly Glu Ala Gly Lys Arg Gly Gly Glu 
        755                 760                 765             


His His Leu Phe Leu Asn Glu Leu Thr Asn Arg Ala His Pro Gly Tyr 
    770                 775                 780                 


Gln Leu Glu Val Phe Asp Arg Asp Ser Ala Leu Phe Gly Ile Ala Phe 
785                 790                 795                 800 


Val Thr Asp Gln Gly Glu Gly Gly Ala Leu Asp Ser Pro His Tyr Glu 
                805                 810                 815     


His Ser His Phe Gln Arg Leu Arg Glu Met Ser Ala Arg Ile Met Ala 
            820                 825                 830         


Gln Ser Ala Pro Phe Glu Pro Ala Leu Pro Ala Leu Arg Asn Pro Val 
        835                 840                 845             


Leu Asp Glu Ser Pro Gly Cys Gln Arg Val Ala Asp Gly Arg Ala Arg 
    850                 855                 860                 


Ala Leu Met Ala Leu Tyr Gln Gly Val Tyr Glu Leu Met Phe Ala Met 
865                 870                 875                 880 


Met Ala Gln His Phe Ala Val Lys Pro Leu Gly Ser Leu Arg Arg Ser 
                885                 890                 895     


Arg Leu Met Asn Ala Ala Ile Asp Leu Met Thr Gly Leu Leu Arg Pro 
            900                 905                 910         


Leu Ser Cys Ala Leu Met Asn Leu Pro Ser Gly Ile Ala Gly Arg Thr 
        915                 920                 925             


Ala Gly Pro Pro Leu Pro Gly Pro Val Asp Thr Arg Ser Tyr Asp Asp 
    930                 935                 940                 


Tyr Ala Leu Gly Cys Arg Met Leu Ala Arg Arg Cys Glu Arg Leu Leu 
945                 950                 955                 960 


Glu Gln Ala Ser Met Leu Glu Pro Gly Trp Leu Pro Asp Ala Gln Met 
                965                 970                 975     


Glu Leu Leu Asp Phe Tyr Arg Arg Gln Met Leu Asp Leu Ala Cys Gly 
            980                 985                 990         


Lys Leu Ser Arg Glu Ala 
        995             


<210>  3
<211>  429
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence for VioC

<400>  3

Met Lys Arg Ala Ile Ile Val Gly Gly Gly Leu Ala Gly Gly Leu Thr 
1               5                   10                  15      


Ala Ile Tyr Leu Ala Lys Arg Gly Tyr Glu Val His Val Val Glu Lys 
            20                  25                  30          


Arg Gly Asp Pro Leu Arg Asp Leu Ser Ser Tyr Val Asp Val Val Ser 
        35                  40                  45              


Ser Arg Ala Ile Gly Val Ser Met Thr Val Arg Gly Ile Lys Ser Val 
    50                  55                  60                  


Leu Ala Ala Gly Ile Pro Arg Ala Glu Leu Asp Ala Cys Gly Glu Pro 
65                  70                  75                  80  


Ile Val Ala Met Ala Phe Ser Val Gly Gly Gln Tyr Arg Met Arg Glu 
                85                  90                  95      


Leu Lys Pro Leu Glu Asp Phe Arg Pro Leu Ser Leu Asn Arg Ala Ala 
            100                 105                 110         


Phe Gln Lys Leu Leu Asn Lys Tyr Ala Asn Leu Ala Gly Val Arg Tyr 
        115                 120                 125             


Tyr Phe Glu His Lys Cys Leu Asp Val Asp Leu Asp Gly Lys Ser Val 
    130                 135                 140                 


Leu Ile Gln Gly Lys Asp Gly Gln Pro Gln Arg Leu Gln Gly Asp Met 
145                 150                 155                 160 


Ile Ile Gly Ala Asp Gly Ala His Ser Ala Val Arg Gln Ala Met Gln 
                165                 170                 175     


Ser Gly Leu Arg Arg Phe Glu Phe Gln Gln Thr Phe Phe Arg His Gly 
            180                 185                 190         


Tyr Lys Thr Leu Val Leu Pro Asp Ala Gln Ala Leu Gly Tyr Arg Lys 
        195                 200                 205             


Asp Thr Leu Tyr Phe Phe Gly Met Asp Ser Gly Gly Leu Phe Ala Gly 
    210                 215                 220                 


Arg Ala Ala Thr Ile Pro Asp Gly Ser Val Ser Ile Ala Val Cys Leu 
225                 230                 235                 240 


Pro Tyr Ser Gly Ser Pro Ser Leu Thr Thr Thr Asp Glu Pro Thr Met 
                245                 250                 255     


Arg Ala Phe Phe Asp Arg Tyr Phe Gly Gly Leu Pro Arg Asp Ala Arg 
            260                 265                 270         


Asp Glu Met Leu Arg Gln Phe Leu Ala Lys Pro Ser Asn Asp Leu Ile 
        275                 280                 285             


Asn Val Arg Ser Ser Thr Phe His Tyr Lys Gly Asn Val Leu Leu Leu 
    290                 295                 300                 


Gly Asp Ala Ala His Ala Thr Ala Pro Phe Leu Gly Gln Gly Met Asn 
305                 310                 315                 320 


Met Ala Leu Glu Asp Ala Arg Thr Phe Val Glu Leu Leu Asp Arg His 
                325                 330                 335     


Gln Gly Asp Gln Asp Lys Ala Phe Pro Glu Phe Thr Glu Leu Arg Lys 
            340                 345                 350         


Val Gln Ala Asp Ala Met Gln Asp Met Ala Arg Ala Asn Tyr Asp Val 
        355                 360                 365             


Leu Ser Cys Ser Asn Pro Ile Phe Phe Met Arg Ala Arg Tyr Thr Arg 
    370                 375                 380                 


Tyr Met His Ser Lys Phe Pro Gly Leu Tyr Pro Pro Asp Met Ala Glu 
385                 390                 395                 400 


Lys Leu Tyr Phe Thr Ser Glu Pro Tyr Asp Arg Leu Gln Gln Ile Gln 
                405                 410                 415     


Arg Lys Gln Asn Val Trp Tyr Lys Ile Gly Arg Val Asn 
            420                 425                 


<210>  4
<211>  373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence for VioD

<400>  4

Met Lys Ile Leu Val Ile Gly Ala Gly Pro Ala Gly Leu Val Phe Ala 
1               5                   10                  15      


Ser Gln Leu Lys Gln Ala Arg Pro Leu Trp Ala Ile Asp Ile Val Glu 
            20                  25                  30          


Lys Asn Asp Glu Gln Glu Val Leu Gly Trp Gly Val Val Leu Pro Gly 
        35                  40                  45              


Arg Pro Gly Gln His Pro Ala Asn Pro Leu Ser Tyr Leu Asp Ala Pro 
    50                  55                  60                  


Glu Arg Leu Asn Pro Gln Phe Leu Glu Asp Phe Lys Leu Val His His 
65                  70                  75                  80  


Asn Glu Pro Ser Leu Met Ser Thr Gly Val Leu Leu Cys Gly Val Glu 
                85                  90                  95      


Arg Arg Gly Leu Val His Ala Leu Arg Asp Lys Cys Arg Ser Gln Gly 
            100                 105                 110         


Ile Ala Ile Arg Phe Glu Ser Pro Leu Leu Glu His Gly Glu Leu Pro 
        115                 120                 125             


Leu Ala Asp Tyr Asp Leu Val Val Leu Ala Asn Gly Val Asn His Lys 
    130                 135                 140                 


Thr Ala His Phe Thr Glu Ala Leu Val Pro Gln Val Asp Tyr Gly Arg 
145                 150                 155                 160 


Asn Lys Tyr Ile Trp Tyr Gly Thr Ser Gln Leu Phe Asp Gln Met Asn 
                165                 170                 175     


Leu Val Phe Arg Thr His Gly Lys Asp Ile Phe Ile Ala His Ala Tyr 
            180                 185                 190         


Lys Tyr Ser Asp Thr Met Ser Thr Phe Ile Val Glu Cys Ser Glu Glu 
        195                 200                 205             


Thr Tyr Ala Arg Ala Arg Leu Gly Glu Met Ser Glu Glu Ala Ser Ala 
    210                 215                 220                 


Glu Tyr Val Ala Lys Val Phe Gln Ala Glu Leu Gly Gly His Gly Leu 
225                 230                 235                 240 


Val Ser Gln Pro Gly Leu Gly Trp Arg Asn Phe Met Thr Leu Ser His 
                245                 250                 255     


Asp Arg Cys His Asp Gly Lys Leu Val Leu Leu Gly Asp Ala Leu Gln 
            260                 265                 270         


Ser Gly His Phe Ser Ile Gly His Gly Thr Thr Met Ala Val Val Val 
        275                 280                 285             


Ala Gln Leu Leu Val Lys Ala Leu Cys Thr Glu Asp Gly Val Pro Ala 
    290                 295                 300                 


Ala Leu Lys Arg Phe Glu Glu Arg Ala Leu Pro Leu Val Gln Leu Phe 
305                 310                 315                 320 


Arg Gly His Ala Asp Asn Ser Arg Val Trp Phe Glu Thr Val Glu Glu 
                325                 330                 335     


Arg Met His Leu Ser Ser Ala Glu Phe Val Gln Ser Phe Asp Ala Arg 
            340                 345                 350         


Arg Lys Ser Leu Pro Pro Met Pro Glu Ala Leu Ala Gln Asn Leu Arg 
        355                 360                 365             


Tyr Ala Leu Gln Arg 
    370             


<210>  5
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence for VioE

<400>  5

Met Glu Asn Arg Glu Pro Pro Leu Leu Pro Ala Arg Trp Ser Ser Ala 
1               5                   10                  15      


Tyr Val Ser Tyr Trp Ser Pro Met Leu Pro Asp Asp Gln Leu Thr Ser 
            20                  25                  30          


Gly Tyr Cys Trp Phe Asp Tyr Glu Arg Asp Ile Cys Arg Ile Asp Gly 
        35                  40                  45              


Leu Phe Asn Pro Trp Ser Glu Arg Asp Thr Gly Tyr Arg Leu Trp Met 
    50                  55                  60                  


Ser Glu Val Gly Asn Ala Ala Ser Gly Arg Thr Trp Lys Gln Lys Val 
65                  70                  75                  80  


Ala Tyr Gly Arg Glu Arg Thr Ala Leu Gly Glu Gln Leu Cys Glu Arg 
                85                  90                  95      


Pro Leu Asp Asp Glu Thr Gly Pro Phe Ala Glu Leu Phe Leu Pro Arg 
            100                 105                 110         


Asp Val Leu Arg Arg Leu Gly Ala Arg His Ile Gly Arg Arg Val Val 
        115                 120                 125             


Leu Gly Arg Glu Ala Asp Gly Trp Arg Tyr Gln Arg Pro Gly Lys Gly 
    130                 135                 140                 


Pro Ser Thr Leu Tyr Leu Asp Ala Ala Ser Gly Thr Pro Leu Arg Met 
145                 150                 155                 160 


Val Thr Gly Asp Glu Ala Ser Arg Ala Ser Leu Arg Asp Phe Pro Asn 
                165                 170                 175     


Val Ser Glu Ala Glu Ile Pro Asp Ala Val Phe Ala Ala Lys Arg 
            180                 185                 190     


<210>  6
<211>  2489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  violacein single operon reading frame comprising the VioA, VioB, 
       VioC, VioD and VioE polypeptides

<400>  6

Met Lys His Ser Ser Asp Ile Cys Ile Val Gly Ala Gly Ile Ser Gly 
1               5                   10                  15      


Leu Thr Cys Ala Ser His Leu Leu Asp Ser Pro Ala Cys Arg Gly Leu 
            20                  25                  30          


Ser Leu Arg Ile Phe Asp Met Gln Gln Glu Ala Gly Gly Arg Ile Arg 
        35                  40                  45              


Ser Lys Met Leu Asp Gly Lys Ala Ser Ile Glu Leu Gly Ala Gly Arg 
    50                  55                  60                  


Tyr Ser Pro Gln Leu His Pro His Phe Gln Ser Ala Met Gln His Tyr 
65                  70                  75                  80  


Ser Gln Lys Ser Glu Val Tyr Pro Phe Thr Gln Leu Lys Phe Lys Ser 
                85                  90                  95      


His Val Gln Gln Lys Leu Lys Arg Ala Met Asn Glu Leu Ser Pro Arg 
            100                 105                 110         


Leu Lys Glu His Gly Lys Glu Ser Phe Leu Gln Phe Val Ser Arg Tyr 
        115                 120                 125             


Gln Gly His Asp Ser Ala Val Gly Met Ile Arg Ser Met Gly Tyr Asp 
    130                 135                 140                 


Ala Leu Phe Leu Pro Asp Ile Ser Ala Glu Met Ala Tyr Asp Ile Val 
145                 150                 155                 160 


Gly Lys His Pro Glu Ile Gln Ser Val Thr Asp Asn Asp Ala Asn Gln 
                165                 170                 175     


Trp Phe Ala Ala Glu Thr Gly Phe Ala Gly Leu Ile Gln Gly Ile Lys 
            180                 185                 190         


Ala Lys Val Lys Ala Ala Gly Ala Arg Phe Ser Leu Gly Tyr Arg Leu 
        195                 200                 205             


Leu Ser Val Arg Thr Asp Gly Asp Gly Tyr Leu Leu Gln Leu Ala Gly 
    210                 215                 220                 


Asp Asp Gly Trp Lys Leu Glu His Arg Thr Arg His Leu Ile Leu Ala 
225                 230                 235                 240 


Ile Pro Pro Ser Ala Met Ala Gly Leu Asn Val Asp Phe Pro Glu Ala 
                245                 250                 255     


Trp Ser Gly Ala Arg Tyr Gly Ser Leu Pro Leu Phe Lys Gly Phe Leu 
            260                 265                 270         


Thr Tyr Gly Glu Pro Trp Trp Leu Asp Tyr Lys Leu Asp Asp Gln Val 
        275                 280                 285             


Leu Ile Val Asp Asn Pro Leu Arg Lys Ile Tyr Phe Lys Gly Asp Lys 
    290                 295                 300                 


Tyr Leu Phe Phe Tyr Thr Asp Ser Glu Met Ala Asn Tyr Trp Arg Gly 
305                 310                 315                 320 


Cys Val Ala Glu Gly Glu Asp Gly Tyr Leu Glu Gln Ile Arg Thr His 
                325                 330                 335     


Leu Ala Ser Ala Leu Gly Ile Val Arg Glu Arg Ile Pro Gln Pro Leu 
            340                 345                 350         


Ala His Val His Lys Tyr Trp Ala His Gly Val Glu Phe Cys Arg Asp 
        355                 360                 365             


Ser Asp Ile Asp His Pro Ser Ala Leu Ser His Arg Asp Ser Gly Ile 
    370                 375                 380                 


Ile Ala Cys Ser Asp Ala Tyr Thr Glu His Cys Gly Trp Met Glu Gly 
385                 390                 395                 400 


Gly Leu Leu Ser Ala Arg Glu Ala Ser Arg Leu Leu Leu Gln Arg Ile 
                405                 410                 415     


Ala Ala Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val 
            420                 425                 430         


Glu Glu Asn Pro Gly Pro Met Ser Ile Leu Asp Phe Pro Arg Ile His 
        435                 440                 445             


Phe Arg Gly Trp Ala Arg Val Asn Ala Pro Thr Ala Asn Arg Asp Pro 
    450                 455                 460                 


His Gly His Ile Asp Met Ala Ser Asn Thr Val Ala Met Ala Gly Glu 
465                 470                 475                 480 


Pro Phe Asp Leu Ala Arg His Pro Thr Glu Phe His Arg His Leu Arg 
                485                 490                 495     


Ser Leu Gly Pro Arg Phe Gly Leu Asp Gly Arg Ala Asp Pro Glu Gly 
            500                 505                 510         


Pro Phe Ser Leu Ala Glu Gly Tyr Asn Ala Ala Gly Asn Asn His Phe 
        515                 520                 525             


Ser Trp Glu Ser Ala Thr Val Ser His Val Gln Trp Asp Gly Gly Glu 
    530                 535                 540                 


Ala Asp Arg Gly Asp Gly Leu Val Gly Ala Arg Leu Ala Leu Trp Gly 
545                 550                 555                 560 


His Tyr Asn Asp Tyr Leu Arg Thr Thr Phe Asn Arg Ala Arg Trp Val 
                565                 570                 575     


Asp Ser Asp Pro Thr Arg Arg Asp Ala Ala Gln Ile Tyr Ala Gly Gln 
            580                 585                 590         


Phe Thr Ile Ser Pro Ala Gly Ala Gly Pro Gly Thr Pro Trp Leu Phe 
        595                 600                 605             


Thr Ala Asp Ile Asp Asp Ser His Gly Ala Arg Trp Thr Arg Gly Gly 
    610                 615                 620                 


His Ile Ala Glu Arg Gly Gly His Phe Leu Asp Glu Glu Phe Gly Leu 
625                 630                 635                 640 


Ala Arg Leu Phe Gln Phe Ser Val Pro Lys Asp His Pro His Phe Leu 
                645                 650                 655     


Phe His Pro Gly Pro Phe Asp Ser Glu Ala Trp Arg Arg Leu Gln Leu 
            660                 665                 670         


Ala Leu Glu Asp Asp Asp Val Leu Gly Leu Thr Val Gln Tyr Ala Leu 
        675                 680                 685             


Phe Asn Met Ser Thr Pro Pro Gln Pro Asn Ser Pro Val Phe His Asp 
    690                 695                 700                 


Met Val Gly Val Val Gly Leu Trp Arg Arg Gly Glu Leu Ala Ser Tyr 
705                 710                 715                 720 


Pro Ala Gly Arg Leu Leu Arg Pro Arg Gln Pro Gly Leu Gly Asp Leu 
                725                 730                 735     


Thr Leu Arg Val Asn Gly Gly Arg Val Ala Leu Asn Leu Ala Cys Ala 
            740                 745                 750         


Ile Pro Phe Ser Thr Arg Ala Ala Gln Pro Ser Ala Pro Asp Arg Leu 
        755                 760                 765             


Thr Pro Asp Leu Gly Ala Lys Leu Pro Leu Gly Asp Leu Leu Leu Arg 
    770                 775                 780                 


Asp Glu Asp Gly Ala Leu Leu Ala Arg Val Pro Gln Ala Leu Tyr Gln 
785                 790                 795                 800 


Asp Tyr Trp Thr Asn His Gly Ile Val Asp Leu Pro Leu Leu Arg Glu 
                805                 810                 815     


Pro Arg Gly Ser Leu Thr Leu Ser Ser Glu Leu Ala Glu Trp Arg Glu 
            820                 825                 830         


Gln Asp Trp Val Thr Gln Ser Asp Ala Ser Asn Leu Tyr Leu Glu Ala 
        835                 840                 845             


Pro Asp Arg Arg His Gly Arg Phe Phe Pro Glu Ser Ile Ala Leu Arg 
    850                 855                 860                 


Ser Tyr Phe Arg Gly Glu Ala Arg Ala Arg Pro Asp Ile Pro His Arg 
865                 870                 875                 880 


Ile Glu Gly Met Gly Leu Val Gly Val Glu Ser Arg Gln Asp Gly Asp 
                885                 890                 895     


Ala Ala Glu Trp Arg Leu Thr Gly Leu Arg Pro Gly Pro Ala Arg Ile 
            900                 905                 910         


Val Leu Asp Asp Gly Ala Glu Ala Ile Pro Leu Arg Val Leu Pro Asp 
        915                 920                 925             


Asp Trp Ala Leu Asp Asp Ala Thr Val Glu Glu Val Asp Tyr Ala Phe 
    930                 935                 940                 


Leu Tyr Arg His Val Met Ala Tyr Tyr Glu Leu Val Tyr Pro Phe Met 
945                 950                 955                 960 


Ser Asp Lys Val Phe Ser Leu Ala Asp Arg Cys Lys Cys Glu Thr Tyr 
                965                 970                 975     


Ala Arg Leu Met Trp Gln Met Cys Asp Pro Gln Asn Arg Asn Lys Ser 
            980                 985                 990         


Tyr Tyr Met Pro Ser Thr Arg Glu  Leu Ser Ala Pro Lys  Ala Arg Leu 
        995                 1000                 1005             


Phe Leu  Lys Tyr Leu Ala His  Val Glu Gly Gln Ala  Arg Leu Gln 
    1010                 1015                 1020             


Ala Pro  Pro Pro Ala Gly Pro  Ala Arg Ile Glu Ser  Lys Ala Gln 
    1025                 1030                 1035             


Leu Ala  Ala Glu Leu Arg Lys  Ala Val Asp Leu Glu  Leu Ser Val 
    1040                 1045                 1050             


Met Leu  Gln Tyr Leu Tyr Ala  Ala Tyr Ser Ile Pro  Asn Tyr Ala 
    1055                 1060                 1065             


Gln Gly  Gln Gln Arg Val Arg  Asp Gly Ala Trp Thr  Ala Glu Gln 
    1070                 1075                 1080             


Leu Gln  Leu Ala Cys Gly Ser  Gly Asp Arg Arg Arg  Asp Gly Gly 
    1085                 1090                 1095             


Ile Arg  Ala Ala Leu Leu Glu  Ile Ala His Glu Glu  Met Ile His 
    1100                 1105                 1110             


Tyr Leu  Val Val Asn Asn Leu  Leu Met Ala Leu Gly  Glu Pro Phe 
    1115                 1120                 1125             


Tyr Ala  Gly Val Pro Leu Met  Gly Glu Ala Ala Arg  Gln Ala Phe 
    1130                 1135                 1140             


Gly Leu  Asp Thr Glu Phe Ala  Leu Glu Pro Phe Ser  Glu Ser Thr 
    1145                 1150                 1155             


Leu Ala  Arg Phe Val Arg Leu  Glu Trp Pro His Phe  Ile Pro Ala 
    1160                 1165                 1170             


Pro Gly  Lys Ser Ile Ala Asp  Cys Tyr Ala Ala Ile  Arg Gln Ala 
    1175                 1180                 1185             


Phe Leu  Asp Leu Pro Asp Leu  Phe Gly Gly Glu Ala  Gly Lys Arg 
    1190                 1195                 1200             


Gly Gly  Glu His His Leu Phe  Leu Asn Glu Leu Thr  Asn Arg Ala 
    1205                 1210                 1215             


His Pro  Gly Tyr Gln Leu Glu  Val Phe Asp Arg Asp  Ser Ala Leu 
    1220                 1225                 1230             


Phe Gly  Ile Ala Phe Val Thr  Asp Gln Gly Glu Gly  Gly Ala Leu 
    1235                 1240                 1245             


Asp Ser  Pro His Tyr Glu His  Ser His Phe Gln Arg  Leu Arg Glu 
    1250                 1255                 1260             


Met Ser  Ala Arg Ile Met Ala  Gln Ser Ala Pro Phe  Glu Pro Ala 
    1265                 1270                 1275             


Leu Pro  Ala Leu Arg Asn Pro  Val Leu Asp Glu Ser  Pro Gly Cys 
    1280                 1285                 1290             


Gln Arg  Val Ala Asp Gly Arg  Ala Arg Ala Leu Met  Ala Leu Tyr 
    1295                 1300                 1305             


Gln Gly  Val Tyr Glu Leu Met  Phe Ala Met Met Ala  Gln His Phe 
    1310                 1315                 1320             


Ala Val  Lys Pro Leu Gly Ser  Leu Arg Arg Ser Arg  Leu Met Asn 
    1325                 1330                 1335             


Ala Ala  Ile Asp Leu Met Thr  Gly Leu Leu Arg Pro  Leu Ser Cys 
    1340                 1345                 1350             


Ala Leu  Met Asn Leu Pro Ser  Gly Ile Ala Gly Arg  Thr Ala Gly 
    1355                 1360                 1365             


Pro Pro  Leu Pro Gly Pro Val  Asp Thr Arg Ser Tyr  Asp Asp Tyr 
    1370                 1375                 1380             


Ala Leu  Gly Cys Arg Met Leu  Ala Arg Arg Cys Glu  Arg Leu Leu 
    1385                 1390                 1395             


Glu Gln  Ala Ser Met Leu Glu  Pro Gly Trp Leu Pro  Asp Ala Gln 
    1400                 1405                 1410             


Met Glu  Leu Leu Asp Phe Tyr  Arg Arg Gln Met Leu  Asp Leu Ala 
    1415                 1420                 1425             


Cys Gly  Lys Leu Ser Arg Glu  Ala Gln Cys Thr Asn  Tyr Ala Leu 
    1430                 1435                 1440             


Leu Lys  Leu Ala Gly Asp Val  Glu Ser Asn Pro Gly  Pro Met Lys 
    1445                 1450                 1455             


Arg Ala  Ile Ile Val Gly Gly  Gly Leu Ala Gly Gly  Leu Thr Ala 
    1460                 1465                 1470             


Ile Tyr  Leu Ala Lys Arg Gly  Tyr Glu Val His Val  Val Glu Lys 
    1475                 1480                 1485             


Arg Gly  Asp Pro Leu Arg Asp  Leu Ser Ser Tyr Val  Asp Val Val 
    1490                 1495                 1500             


Ser Ser  Arg Ala Ile Gly Val  Ser Met Thr Val Arg  Gly Ile Lys 
    1505                 1510                 1515             


Ser Val  Leu Ala Ala Gly Ile  Pro Arg Ala Glu Leu  Asp Ala Cys 
    1520                 1525                 1530             


Gly Glu  Pro Ile Val Ala Met  Ala Phe Ser Val Gly  Gly Gln Tyr 
    1535                 1540                 1545             


Arg Met  Arg Glu Leu Lys Pro  Leu Glu Asp Phe Arg  Pro Leu Ser 
    1550                 1555                 1560             


Leu Asn  Arg Ala Ala Phe Gln  Lys Leu Leu Asn Lys  Tyr Ala Asn 
    1565                 1570                 1575             


Leu Ala  Gly Val Arg Tyr Tyr  Phe Glu His Lys Cys  Leu Asp Val 
    1580                 1585                 1590             


Asp Leu  Asp Gly Lys Ser Val  Leu Ile Gln Gly Lys  Asp Gly Gln 
    1595                 1600                 1605             


Pro Gln  Arg Leu Gln Gly Asp  Met Ile Ile Gly Ala  Asp Gly Ala 
    1610                 1615                 1620             


His Ser  Ala Val Arg Gln Ala  Met Gln Ser Gly Leu  Arg Arg Phe 
    1625                 1630                 1635             


Glu Phe  Gln Gln Thr Phe Phe  Arg His Gly Tyr Lys  Thr Leu Val 
    1640                 1645                 1650             


Leu Pro  Asp Ala Gln Ala Leu  Gly Tyr Arg Lys Asp  Thr Leu Tyr 
    1655                 1660                 1665             


Phe Phe  Gly Met Asp Ser Gly  Gly Leu Phe Ala Gly  Arg Ala Ala 
    1670                 1675                 1680             


Thr Ile  Pro Asp Gly Ser Val  Ser Ile Ala Val Cys  Leu Pro Tyr 
    1685                 1690                 1695             


Ser Gly  Ser Pro Ser Leu Thr  Thr Thr Asp Glu Pro  Thr Met Arg 
    1700                 1705                 1710             


Ala Phe  Phe Asp Arg Tyr Phe  Gly Gly Leu Pro Arg  Asp Ala Arg 
    1715                 1720                 1725             


Asp Glu  Met Leu Arg Gln Phe  Leu Ala Lys Pro Ser  Asn Asp Leu 
    1730                 1735                 1740             


Ile Asn  Val Arg Ser Ser Thr  Phe His Tyr Lys Gly  Asn Val Leu 
    1745                 1750                 1755             


Leu Leu  Gly Asp Ala Ala His  Ala Thr Ala Pro Phe  Leu Gly Gln 
    1760                 1765                 1770             


Gly Met  Asn Met Ala Leu Glu  Asp Ala Arg Thr Phe  Val Glu Leu 
    1775                 1780                 1785             


Leu Asp  Arg His Gln Gly Asp  Gln Asp Lys Ala Phe  Pro Glu Phe 
    1790                 1795                 1800             


Thr Glu  Leu Arg Lys Val Gln  Ala Asp Ala Met Gln  Asp Met Ala 
    1805                 1810                 1815             


Arg Ala  Asn Tyr Asp Val Leu  Ser Cys Ser Asn Pro  Ile Phe Phe 
    1820                 1825                 1830             


Met Arg  Ala Arg Tyr Thr Arg  Tyr Met His Ser Lys  Phe Pro Gly 
    1835                 1840                 1845             


Leu Tyr  Pro Pro Asp Met Ala  Glu Lys Leu Tyr Phe  Thr Ser Glu 
    1850                 1855                 1860             


Pro Tyr  Asp Arg Leu Gln Gln  Ile Gln Arg Lys Gln  Asn Val Trp 
    1865                 1870                 1875             


Tyr Lys  Ile Gly Arg Val Asn  Arg Ala Glu Gly Arg  Gly Ser Leu 
    1880                 1885                 1890             


Leu Thr  Cys Gly Asp Val Glu  Glu Asn Pro Gly Pro  Met Lys Ile 
    1895                 1900                 1905             


Leu Val  Ile Gly Ala Gly Pro  Ala Gly Leu Val Phe  Ala Ser Gln 
    1910                 1915                 1920             


Leu Lys  Gln Ala Arg Pro Leu  Trp Ala Ile Asp Ile  Val Glu Lys 
    1925                 1930                 1935             


Asn Asp  Glu Gln Glu Val Leu  Gly Trp Gly Val Val  Leu Pro Gly 
    1940                 1945                 1950             


Arg Pro  Gly Gln His Pro Ala  Asn Pro Leu Ser Tyr  Leu Asp Ala 
    1955                 1960                 1965             


Pro Glu  Arg Leu Asn Pro Gln  Phe Leu Glu Asp Phe  Lys Leu Val 
    1970                 1975                 1980             


His His  Asn Glu Pro Ser Leu  Met Ser Thr Gly Val  Leu Leu Cys 
    1985                 1990                 1995             


Gly Val  Glu Arg Arg Gly Leu  Val His Ala Leu Arg  Asp Lys Cys 
    2000                 2005                 2010             


Arg Ser  Gln Gly Ile Ala Ile  Arg Phe Glu Ser Pro  Leu Leu Glu 
    2015                 2020                 2025             


His Gly  Glu Leu Pro Leu Ala  Asp Tyr Asp Leu Val  Val Leu Ala 
    2030                 2035                 2040             


Asn Gly  Val Asn His Lys Thr  Ala His Phe Thr Glu  Ala Leu Val 
    2045                 2050                 2055             


Pro Gln  Val Asp Tyr Gly Arg  Asn Lys Tyr Ile Trp  Tyr Gly Thr 
    2060                 2065                 2070             


Ser Gln  Leu Phe Asp Gln Met  Asn Leu Val Phe Arg  Thr His Gly 
    2075                 2080                 2085             


Lys Asp  Ile Phe Ile Ala His  Ala Tyr Lys Tyr Ser  Asp Thr Met 
    2090                 2095                 2100             


Ser Thr  Phe Ile Val Glu Cys  Ser Glu Glu Thr Tyr  Ala Arg Ala 
    2105                 2110                 2115             


Arg Leu  Gly Glu Met Ser Glu  Glu Ala Ser Ala Glu  Tyr Val Ala 
    2120                 2125                 2130             


Lys Val  Phe Gln Ala Glu Leu  Gly Gly His Gly Leu  Val Ser Gln 
    2135                 2140                 2145             


Pro Gly  Leu Gly Trp Arg Asn  Phe Met Thr Leu Ser  His Asp Arg 
    2150                 2155                 2160             


Cys His  Asp Gly Lys Leu Val  Leu Leu Gly Asp Ala  Leu Gln Ser 
    2165                 2170                 2175             


Gly His  Phe Ser Ile Gly His  Gly Thr Thr Met Ala  Val Val Val 
    2180                 2185                 2190             


Ala Gln  Leu Leu Val Lys Ala  Leu Cys Thr Glu Asp  Gly Val Pro 
    2195                 2200                 2205             


Ala Ala  Leu Lys Arg Phe Glu  Glu Arg Ala Leu Pro  Leu Val Gln 
    2210                 2215                 2220             


Leu Phe  Arg Gly His Ala Asp  Asn Ser Arg Val Trp  Phe Glu Thr 
    2225                 2230                 2235             


Val Glu  Glu Arg Met His Leu  Ser Ser Ala Glu Phe  Val Gln Ser 
    2240                 2245                 2250             


Phe Asp  Ala Arg Arg Lys Ser  Leu Pro Pro Met Pro  Glu Ala Leu 
    2255                 2260                 2265             


Ala Gln  Asn Leu Arg Tyr Ala  Leu Gln Arg Arg Ala  Glu Gly Arg 
    2270                 2275                 2280             


Gly Ser  Leu Leu Thr Cys Gly  Asp Val Glu Glu Asn  Pro Gly Pro 
    2285                 2290                 2295             


Met Glu  Asn Arg Glu Pro Pro  Leu Leu Pro Ala Arg  Trp Ser Ser 
    2300                 2305                 2310             


Ala Tyr  Val Ser Tyr Trp Ser  Pro Met Leu Pro Asp  Asp Gln Leu 
    2315                 2320                 2325             


Thr Ser  Gly Tyr Cys Trp Phe  Asp Tyr Glu Arg Asp  Ile Cys Arg 
    2330                 2335                 2340             


Ile Asp  Gly Leu Phe Asn Pro  Trp Ser Glu Arg Asp  Thr Gly Tyr 
    2345                 2350                 2355             


Arg Leu  Trp Met Ser Glu Val  Gly Asn Ala Ala Ser  Gly Arg Thr 
    2360                 2365                 2370             


Trp Lys  Gln Lys Val Ala Tyr  Gly Arg Glu Arg Thr  Ala Leu Gly 
    2375                 2380                 2385             


Glu Gln  Leu Cys Glu Arg Pro  Leu Asp Asp Glu Thr  Gly Pro Phe 
    2390                 2395                 2400             


Ala Glu  Leu Phe Leu Pro Arg  Asp Val Leu Arg Arg  Leu Gly Ala 
    2405                 2410                 2415             


Arg His  Ile Gly Arg Arg Val  Val Leu Gly Arg Glu  Ala Asp Gly 
    2420                 2425                 2430             


Trp Arg  Tyr Gln Arg Pro Gly  Lys Gly Pro Ser Thr  Leu Tyr Leu 
    2435                 2440                 2445             


Asp Ala  Ala Ser Gly Thr Pro  Leu Arg Met Val Thr  Gly Asp Glu 
    2450                 2455                 2460             


Ala Ser  Arg Ala Ser Leu Arg  Asp Phe Pro Asn Val  Ser Glu Ala 
    2465                 2470                 2475             


Glu Ile  Pro Asp Ala Val Phe  Ala Ala Lys Arg 
    2480                 2485                 


<210>  7
<211>  7467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  violacein ORF DNA sequence

<400>  7
atgaaacact cttctgatat ttgtatagtt ggggcaggga tatcaggcct cacctgtgct       60

tcacaccttc ttgatagccc agcttgcagg ggcctgtcac ttcgaatttt tgacatgcaa      120

caggaggccg gcggacggat ccgctctaag atgcttgatg gcaaggcgtc tatcgaactc      180

ggcgccggac ggtactctcc gcaacttcac ccccacttcc aaagtgcaat gcaacactac      240

agtcaaaaat ccgaggtcta cccattcacc caattgaagt tcaaatccca tgttcaacag      300

aaactcaaac gggccatgaa cgaactgtca ccgcgcctta aggagcacgg aaaggagagc      360

tttctccagt ttgtgtctcg ctaccagggt catgactccg ctgtagggat gattaggtcc      420

atggggtatg atgccctctt tctcccggat atatcagctg aaatggctta tgacattgtt      480

ggcaagcatc ccgaaattca gtctgtcacg gacaacgatg ccaaccagtg gtttgcagca      540

gaaacaggct ttgcgggcct tatacaggga attaaagcca aagtaaaggc cgctggtgct      600

cgattctcac ttggctatcg actcctcagt gttaggacag atggtgatgg ctatctcttg      660

caattggccg gcgacgatgg ttggaagttg gagcaccgaa cccgccactt gatcctcgcc      720

atcccacctt ctgcaatggc tggacttaac gtcgacttcc ctgaagcttg gtcaggggca      780

cgatatggct cactccctct cttcaaaggg ttccttactt acggagagcc ttggtggctt      840

gactataagc ttgacgacca ggttctcatt gtagataatc cgctcaggaa gatttatttc      900

aaaggcgaca agtacctctt cttctatact gattctgaga tggctaacta ttggaggggc      960

tgcgtagcgg aaggggagga cgggtatctg gaacaaatac gaacccacct ggccagtgcc     1020

cttggcatag tacgggagcg gataccacag cctctcgctc atgtgcacaa gtattgggcg     1080

catggtgtcg aattctgccg cgactctgac atcgatcacc cctccgccct gagtcacagg     1140

gattcaggta ttattgcttg cagcgatgcg tataccgaac attgcggttg gatggaagga     1200

ggtctgctgt ctgcccgaga agcctcccga ctgctccttc agagaatcgc ggcaagagca     1260

gaagggcggg ggagccttct tacatgtgga gacgtggagg aaaatccagg acctatgtca     1320

attctggatt ttccgcgcat ccattttaga ggctgggcga gagtcaacgc tccaacagcc     1380

aaccgggacc cgcatggcca catcgatatg gcgtctaaca cagtggcaat ggcaggggag     1440

ccattcgatc ttgctagaca cccgacagag ttccatcgac atttgcgaag tttgggaccg     1500

cggttcggcc tcgacgggag agcagacccg gaaggtccgt tctctcttgc ggaggggtat     1560

aatgccgcag gcaacaatca cttttcttgg gaatctgcta cggtatccca tgtgcaatgg     1620

gatgggggtg aagcagaccg aggtgatggg cttgtcggcg caagactcgc actgtgggga     1680

cactataacg attacttgcg caccaccttc aaccgagcgc gatgggtcga cagcgatccg     1740

acccggcggg atgccgctca gatatatgct gggcaattta ccatttcccc agccggggcc     1800

gggccaggga cgccatggtt gttcacggca gacattgatg actcccatgg cgcccggtgg     1860

acccgaggag gtcacatcgc ggaaaggggg ggtcattttt tggacgagga atttggcctg     1920

gcaagacttt ttcaattctc cgttccgaaa gaccacccac attttctttt ccatcctgga     1980

cctttcgatt ccgaagcttg gagaaggctg caactggcgt tggaggacga cgatgtactg     2040

ggcctgactg tccagtacgc tctttttaac atgagtactc caccacaacc caacagccca     2100

gtcttccacg atatggtagg agtggttggg ttgtggagaa gaggagagct cgcaagctat     2160

cccgcgggac gactgcttcg cccccgacag ccggggctcg gagatcttac gcttagagtc     2220

aacggcggca gagttgctct taacctcgca tgcgcaattc cattctctac tcgggcagct     2280

cagccctccg ctccggatag gttgacacct gacctcggag caaaactgcc gctcggcgat     2340

cttctcctta gggacgagga cggtgcgctg ctggccaggg taccccaagc gctttaccaa     2400

gattactgga cgaaccatgg aatagtggac ttgcctctcc ttcgggaacc tagaggctca     2460

cttacattgt cctccgagct ggcagagtgg agggaacagg actgggttac acaaagcgac     2520

gcgtccaatt tgtatcttga agctcctgac cggcgccatg ggcgattttt tccggaaagt     2580

atagcgctca ggagctattt cagaggtgaa gcaagggcgc gaccggacat tccccatcgg     2640

attgaaggca tgggcctcgt gggggtcgag agccggcagg acggggatgc cgcagaatgg     2700

cgcttgacag gattgaggcc gggtccggca aggattgtgc tggatgatgg ggccgaggca     2760

attccattgc gagtactgcc cgatgactgg gctttggacg atgcgactgt cgaagaagta     2820

gattacgcgt ttctttacag gcacgttatg gcttactacg aactggtata cccatttatg     2880

agcgataagg tattctcact ggccgaccga tgcaaatgcg agacgtacgc gcgcctgatg     2940

tggcaaatgt gtgatcctca gaatcgcaat aaaagttact acatgccgag tacgcgcgag     3000

ctcagcgcac caaaggctcg cctgtttctg aagtacttgg cccatgtgga agggcaggcg     3060

aggttgcaag ctcccccacc agccgggccc gccagaatag aaagtaaagc ccaattggcc     3120

gcagagttgc gcaaagccgt cgatttggaa ctctccgtca tgcttcaata tctctacgca     3180

gcgtattcta taccgaacta cgcacagggt caacaaagag tcagagacgg tgcgtggacc     3240

gccgaacagc ttcaacttgc atgcggtagc ggtgataggc gaagggacgg tggtatacgc     3300

gcggcattgt tggaaattgc ccacgaagaa atgatacatt acctcgtggt caacaatctt     3360

ctcatggcgc tgggcgaacc attctatgcc ggcgtgcccc ttatggggga agcagctagg     3420

caagctttcg gcctggacac agaatttgct cttgagccgt tttccgagtc aactttggca     3480

cgattcgtcc ggttggaatg gccacacttt atcccagccc caggaaagag tatagcggat     3540

tgttatgctg caatccgaca ggcttttctt gatctccccg atctctttgg cggtgaggcc     3600

gggaaacgag gtggcgagca ccacctcttc ttgaatgaat tgaccaaccg cgcacacccg     3660

ggttaccaac tggaagtatt tgatagggat agcgcgttgt ttggaatagc gtttgtcacc     3720

gatcaaggtg aaggcggtgc actcgacagt ccgcactatg aacactccca ctttcagcgg     3780

ttgcgggaaa tgagcgcacg gataatggct caatccgctc ccttcgaacc tgcccttccg     3840

gccctcagaa accccgttct cgatgagagc ccaggctgcc aacgggtggc cgacgggcgc     3900

gcacgcgcgc tgatggcact gtaccagggg gtgtacgaac tgatgttcgc aatgatggct     3960

cagcactttg ctgtaaaacc gctcgggagt cttcgaaggt ccaggttgat gaatgccgca     4020

attgatttga tgaccgggct cctccgccct ttgtcatgtg ctctcatgaa tttgccttca     4080

ggtatagcgg ggcgcaccgc aggaccgcca cttccaggac ccgttgacac gcgaagctac     4140

gacgattatg ccctgggctg ccgaatgctg gcacgacgct gcgaacgact gcttgagcaa     4200

gcgtccatgc tggaacccgg atggcttccc gacgcccaga tggaactcct ggatttctat     4260

cgacgccaga tgctggatct tgcgtgcggg aagctgagta gggaggcgca gtgtactaac     4320

tatgctctgt tgaaattggc tggggatgtc gaatccaatc caggccctat gaaacgagca     4380

atcattgtcg gcggcggcct cgccggtggc ctgacagcca tctatttggc taaacgcggg     4440

tatgaggtcc atgtagtaga gaagagaggt gatcctttgc gagatttgag cagctatgtt     4500

gacgtggtat cttcccgggc catcggtgtc agtatgacgg tcagaggcat aaaatccgtg     4560

ttggcggccg gtatcccacg cgccgaactg gatgcttgtg gcgagccaat tgtagcaatg     4620

gcattctccg taggcgggca ataccgaatg cgggaactta aaccgctcga ggatttccgg     4680

ccactgtcat tgaatcgggc tgcgttccaa aaactgctta ataaatacgc aaaccttgca     4740

ggcgttaggt attatttcga gcacaagtgt ctcgatgtcg atttggacgg gaaaagtgtt     4800

ctgattcaag gaaaagacgg gcaaccgcag cgccttcagg gtgacatgat aataggcgcg     4860

gacggcgcgc acagcgccgt acgacaggcc atgcaatctg gactccggcg gtttgaattc     4920

cagcaaacat ttttccgcca tgggtataag actttggttc tgcctgatgc gcaagctttg     4980

gggtatcgga aagatacgct ctatttcttt gggatggata gtggagggct tttcgccgga     5040

cgcgctgcta cgattcccga cggaagtgtc tcaatagcag tctgtcttcc gtacagtgga     5100

tccccgagcc ttacgactac ggatgaaccg accatgcggg cgtttttcga ccgctacttc     5160

ggaggtttgc cgagagatgc tcgggacgaa atgctcaggc aattccttgc caaaccgagt     5220

aacgatttga tcaacgtgcg gtcttccaca tttcactata aaggtaacgt gctgttgctg     5280

ggcgacgcag cccacgcaac agcaccgttc ctggggcaag ggatgaatat ggcattggaa     5340

gacgcgagaa cgttcgtcga gttgcttgat cgccaccaag gtgatcagga taaagcgttt     5400

ccggaattta cagagcttag gaaggttcaa gccgatgcta tgcaagacat ggcacgagcg     5460

aactatgatg tgctcagctg tagtaacccg atctttttta tgagagcaag atatacgagg     5520

tacatgcata gtaaattccc aggtctgtac ccccccgata tggctgagaa actctatttc     5580

acgtctgagc cgtatgatcg attgcaacag atccagcgaa aacaaaatgt atggtataag     5640

attggtcgcg ttaatcgagc agaagggcga gggtcactgt tgacatgtgg tgacgtggaa     5700

gagaaccccg gccctatgaa gatcctcgtc atcggcgcgg gaccagccgg tttggtgttt     5760

gcgtcccaac ttaaacaggc gaggcccctg tgggcgatag atatcgtcga aaaaaacgat     5820

gaacaagagg tgcttggatg gggggtggtc ttgcctggta gaccgggtca gcaccctgcg     5880

aatccgctta gctacctcga cgcgcccgag aggctgaacc ctcagttcct tgaagacttc     5940

aaactggtgc atcataatga accaagtctc atgtctaccg gagtactttt gtgcggggtc     6000

gagagacggg gcctggtcca tgctctgcgg gataagtgca ggtcccaagg tatagctatt     6060

aggtttgaaa gtccattgct tgaacatggc gaacttccct tggcggatta tgatcttgtg     6120

gtactcgcaa acggagtgaa ccataagacc gcgcatttta ccgaggctct ggttcctcag     6180

gtcgactatg gtcgaaacaa gtacatttgg tacggcacct cccaactttt cgatcaaatg     6240

aacctggtat ttaggacgca cggcaaagac attttcattg ctcatgcgta taaatactcc     6300

gacaccatgt ccacgtttat tgtcgagtgc tctgaggaga cgtacgctag ggcccggctg     6360

ggcgaaatga gtgaggaagc atcagcagaa tacgtcgcca aggttttcca agcagaactc     6420

ggagggcatg ggctggtaag ccaacccgga ttgggatgga ggaacttcat gactcttagc     6480

cacgatcgct gccatgacgg aaaactcgtg ttgttggggg acgcactcca gagcggtcac     6540

tttagtattg gacacggtac cacgatggct gttgtggtag cacagttgct tgtcaaagcg     6600

ttgtgcacag aggatggtgt acccgcagcg cttaagcgct tcgaggagag ggctctgccc     6660

ctggttcaac ttttccgcgg tcatgcggac aacagccggg tatggtttga aacagttgag     6720

gagcgaatgc acttgtcctc cgctgaattt gtccaaagct ttgatgcccg ccggaaaagt     6780

cttccgccta tgcctgaagc gcttgctcag aatcttcgat atgccctcca gaggagggcc     6840

gaggggcggg gctcacttct tacgtgcggt gacgtagaag aaaatcccgg gcctatggaa     6900

aaccgggaac ctcccttgtt gccagcacgg tggtcctccg catatgtctc ctactggtca     6960

ccgatgttgc cagacgatca gctgacctca gggtactgtt ggtttgatta tgagagagac     7020

atctgcagaa ttgacggtct ttttaacccc tggtctgaga gagataccgg ttacagactg     7080

tggatgtctg aagtagggaa tgcagcgagt ggtaggacct ggaagcaaaa agtggcatac     7140

ggcagggagc gaacggcttt gggagaacag ctttgcgagc gaccattgga tgacgaaaca     7200

ggcccctttg ccgagttgtt cctgccacga gacgtattgc gcagacttgg agcacgacat     7260

ataggacgcc gggtagttct gggcagggaa gccgatggat ggagatatca gcgaccagga     7320

aaagggccaa gtaccctgta tctggatgca gccagcggga ccccacttcg gatggtcact     7380

ggagacgaag cgagtcgcgc ttccttgagg gattttccca acgtttccga agcggagata     7440

ccggatgctg tttttgccgc caagcgc                                         7467


<210>  8
<211>  594
<212>  PRT
<213>  Valeriana officinalis


<220>
<221>  misc_feature
<222>  (228)..(228)
<223>  Xaa can be any naturally occurring amino acid

<400>  8

Met Ile Thr Ser Ser Ser Ser Val Arg Ser Leu Cys Cys Pro Lys Thr 
1               5                   10                  15      


Ser Ile Ile Ser Gly Lys Leu Leu Pro Ser Leu Leu Leu Thr Asn Val 
            20                  25                  30          


Ile Asn Val Ser Asn Gly Thr Ser Ser Arg Ala Cys Val Ser Met Ser 
        35                  40                  45              


Ser Leu Pro Val Ser Lys Ser Thr Ala Ser Ser Ile Ala Ala Pro Leu 
    50                  55                  60                  


Val Arg Asp Asn Gly Ser Ala Leu Asn Phe Phe Pro Gln Ala Pro Gln 
65                  70                  75                  80  


Val Glu Ile Asp Glu Ser Ser Arg Ile Met Glu Leu Val Glu Ala Thr 
                85                  90                  95      


Arg Arg Thr Leu Arg Asn Glu Ser Ser Asp Ser Thr Glu Lys Met Arg 
            100                 105                 110         


Leu Ile Asp Ser Leu Gln Arg Leu Gly Leu Asn His His Phe Glu Gln 
        115                 120                 125             


Asp Ile Lys Glu Met Leu Gln Asp Phe Ala Asn Glu His Lys Asn Thr 
    130                 135                 140                 


Asn Gln Asp Leu Phe Thr Thr Ser Leu Arg Phe Arg Leu Leu Arg His 
145                 150                 155                 160 


Asn Gly Phe Asn Val Thr Pro Asp Val Phe Asn Lys Phe Thr Glu Glu 
                165                 170                 175     


Asn Gly Lys Phe Lys Glu Ser Leu Gly Glu Asp Thr Ile Gly Ile Leu 
            180                 185                 190         


Ser Leu Tyr Glu Ala Ser Tyr Leu Gly Gly Lys Gly Glu Glu Ile Leu 
        195                 200                 205             


Ser Glu Ala Met Lys Phe Ser Glu Ser Lys Leu Arg Glu Ser Ser Gly 
    210                 215                 220                 


His Val Ala Xaa His Ile Arg Arg Gln Ile Phe Gln Ser Leu Glu Leu 
225                 230                 235                 240 


Pro Arg His Leu Arg Met Ala Arg Leu Glu Ser Arg Arg Tyr Ile Glu 
                245                 250                 255     


Glu Asp Tyr Ser Asn Glu Ile Gly Ala Asp Ser Ser Leu Leu Glu Leu 
            260                 265                 270         


Ala Lys Leu Asp Phe Asn Ser Val Gln Ala Leu His Gln Met Glu Leu 
        275                 280                 285             


Thr Glu Ile Ser Arg Trp Trp Lys Gln Leu Gly Leu Ser Asp Lys Leu 
    290                 295                 300                 


Pro Phe Ala Arg Asp Arg Pro Leu Glu Cys Phe Leu Trp Thr Val Gly 
305                 310                 315                 320 


Leu Leu Pro Glu Pro Lys Tyr Ser Gly Cys Arg Ile Glu Leu Ala Lys 
                325                 330                 335     


Thr Ile Ala Val Leu Leu Val Ile Asp Asp Ile Phe Asp Thr Tyr Gly 
            340                 345                 350         


Ser Tyr Asp Gln Leu Ile Leu Phe Thr Asn Ala Ile Arg Arg Trp Asp 
        355                 360                 365             


Leu Asp Ala Met Asp Glu Leu Pro Glu Tyr Met Lys Ile Cys Tyr Met 
    370                 375                 380                 


Ala Leu Tyr Asn Thr Thr Asn Glu Ile Cys Tyr Lys Val Leu Lys Glu 
385                 390                 395                 400 


Asn Gly Trp Ser Val Leu Pro Tyr Leu Glu Arg Thr Trp Ile Asp Met 
                405                 410                 415     


Val Glu Gly Phe Met Leu Glu Ala Lys Trp Leu Asn Ser Gly Glu Gln 
            420                 425                 430         


Pro Asn Leu Glu Ala Tyr Ile Glu Asn Gly Val Thr Thr Ala Gly Ser 
        435                 440                 445             


Tyr Met Ala Leu Val His Leu Phe Phe Leu Ile Gly Asp Gly Val Asn 
    450                 455                 460                 


Asp Glu Asn Val Lys Leu Leu Leu Asp Pro Tyr Pro Lys Leu Phe Ser 
465                 470                 475                 480 


Ser Ala Gly Arg Ile Leu Arg Leu Trp Asp Asp Leu Gly Thr Ala Lys 
                485                 490                 495     


Glu Glu Gln Glu Arg Gly Asp Val Ser Ser Ser Ile Gln Leu Tyr Met 
            500                 505                 510         


Lys Glu Lys Asn Val Arg Ser Glu Ser Glu Gly Arg Glu Gly Ile Val 
        515                 520                 525             


Glu Ile Ile Tyr Asn Leu Trp Lys Asp Met Asn Gly Glu Leu Ile Gly 
    530                 535                 540                 


Ser Asn Ala Leu Pro Gln Ala Ile Ile Glu Thr Ser Phe Asn Met Ala 
545                 550                 555                 560 


Arg Thr Ser Gln Val Val Tyr Gln His Glu Asp Asp Thr Tyr Phe Ser 
                565                 570                 575     


Ser Val Asp Asn Tyr Val Gln Ser Leu Phe Phe Thr Pro Val Ser Val 
            580                 585                 590         


Ser Val 
        


<210>  9
<211>  718
<212>  PRT
<213>  Aspergillus clavatus

<400>  9

Met Ala Cys Lys Tyr Ser Thr Leu Ile Asp Ser Ser Leu Tyr Asp Arg 
1               5                   10                  15      


Glu Gly Leu Cys Pro Gly Ile Asp Leu Arg Arg His Val Ala Gly Glu 
            20                  25                  30          


Leu Glu Glu Val Gly Ala Phe Arg Ala Gln Glu Asp Trp Arg Arg Leu 
        35                  40                  45              


Val Gly Pro Leu Pro Lys Pro Tyr Ala Gly Leu Leu Gly Pro Asp Phe 
    50                  55                  60                  


Ser Phe Ile Thr Gly Ala Val Pro Glu Cys His Pro Asp Arg Met Glu 
65                  70                  75                  80  


Ile Val Ala Tyr Ala Leu Glu Phe Gly Phe Met His Asp Asp Val Ile 
                85                  90                  95      


Asp Thr Asp Val Asn His Ala Ser Leu Asp Glu Val Gly His Thr Leu 
            100                 105                 110         


Asp Gln Ser Arg Thr Gly Lys Ile Glu Asp Lys Gly Ser Asp Gly Lys 
        115                 120                 125             


Arg Gln Met Val Thr Gln Ile Ile Arg Glu Met Met Ala Ile Asp Pro 
    130                 135                 140                 


Glu Arg Ala Met Thr Val Ala Lys Ser Trp Ala Ser Gly Val Arg His 
145                 150                 155                 160 


Ser Ser Arg Arg Lys Glu Asp Thr Asn Phe Lys Ala Leu Glu Gln Tyr 
                165                 170                 175     


Ile Pro Tyr Arg Ala Leu Asp Val Gly Tyr Met Leu Trp His Gly Leu 
            180                 185                 190         


Val Thr Phe Gly Cys Ala Ile Thr Ile Pro Asn Glu Glu Glu Glu Glu 
        195                 200                 205             


Ala Lys Arg Leu Ile Ile Pro Ala Leu Val Gln Ala Ser Leu Leu Asn 
    210                 215                 220                 


Asp Leu Phe Ser Phe Glu Lys Glu Lys Asn Asp Ala Asn Val Gln Asn 
225                 230                 235                 240 


Ala Val Leu Ile Val Met Asn Glu His Gly Cys Ser Glu Glu Glu Ala 
                245                 250                 255     


Arg Asp Ile Leu Lys Lys Arg Ile Arg Leu Glu Cys Ala Asn Tyr Leu 
            260                 265                 270         


Arg Asn Val Lys Glu Thr Asn Ala Arg Ala Asp Val Ser Asp Glu Leu 
        275                 280                 285             


Lys Arg Tyr Ile Asn Val Met Gln Tyr Thr Leu Ser Gly Asn Ala Ala 
    290                 295                 300                 


Trp Ser Thr Asn Cys Pro Arg Tyr Asn Gly Pro Thr Lys Phe Asn Glu 
305                 310                 315                 320 


Leu Gln Leu Leu Arg Ser Glu His Gly Leu Ala Lys Tyr Pro Ser Arg 
                325                 330                 335     


Trp Ser Gln Glu Asn Arg Thr Ser Gly Leu Val Glu Gly Asp Cys His 
            340                 345                 350         


Glu Ser Lys Pro Asn Glu Leu Lys Arg Lys Arg Asn Gly Val Ser Val 
        355                 360                 365             


Asp Asp Glu Met Arg Thr Asn Gly Thr Asn Gly Ala Lys Lys Pro Ala 
    370                 375                 380                 


His Val Ser Gln Pro Ser Thr Asp Ser Ile Val Leu Glu Asp Met Val 
385                 390                 395                 400 


Gln Leu Ala Arg Thr Cys Asp Leu Pro Asp Leu Ser Asp Thr Val Ile 
                405                 410                 415     


Leu Gln Pro Tyr Arg Tyr Leu Thr Ser Leu Pro Ser Lys Gly Phe Arg 
            420                 425                 430         


Asp Gln Ala Ile Asp Ser Ile Asn Lys Trp Leu Lys Val Pro Pro Lys 
        435                 440                 445             


Ser Val Lys Met Ile Lys Asp Val Val Lys Met Leu His Ser Ala Ser 
    450                 455                 460                 


Leu Met Leu Asp Asp Leu Glu Asp Asn Ser Pro Leu Arg Arg Gly Lys 
465                 470                 475                 480 


Pro Ser Thr His Ser Ile Tyr Gly Met Ala Gln Thr Val Asn Ser Ala 
                485                 490                 495     


Thr Tyr Gln Tyr Ile Thr Ala Thr Asp Ile Thr Ala Gln Leu Gln Asn 
            500                 505                 510         


Ser Glu Thr Phe His Ile Phe Val Glu Glu Leu Gln Gln Leu His Val 
        515                 520                 525             


Gly Gln Ser Tyr Asp Leu Tyr Trp Thr His Asn Thr Leu Cys Pro Thr 
    530                 535                 540                 


Ile Ala Glu Tyr Leu Lys Met Val Asp Met Lys Thr Gly Gly Leu Phe 
545                 550                 555                 560 


Arg Met Leu Thr Arg Met Met Ile Ala Glu Ser Pro Val Val Asp Lys 
                565                 570                 575     


Val Pro Asn Ser Asp Met Asn Leu Phe Ser Cys Leu Ile Gly Arg Phe 
            580                 585                 590         


Phe Gln Ile Arg Asp Asp Tyr Gln Asn Leu Ala Ser Ala Asp Tyr Ala 
        595                 600                 605             


Lys Ala Lys Gly Phe Ala Glu Asp Leu Asp Glu Gly Lys Tyr Ser Phe 
    610                 615                 620                 


Thr Leu Ile His Cys Ile Gln Thr Leu Glu Ser Lys Pro Glu Leu Ala 
625                 630                 635                 640 


Gly Glu Met Met Gln Leu Arg Ala Phe Leu Met Lys Arg Arg His Glu 
                645                 650                 655     


Gly Lys Leu Ser Gln Glu Ala Lys Gln Glu Val Leu Val Thr Met Lys 
            660                 665                 670         


Lys Thr Glu Ser Leu Gln Tyr Thr Leu Ser Val Leu Arg Glu Leu His 
        675                 680                 685             


Ser Glu Leu Glu Lys Glu Val Glu Asn Leu Glu Ala Lys Phe Gly Glu 
    690                 695                 700                 


Glu Asn Phe Thr Leu Arg Val Met Leu Glu Leu Leu Lys Val 
705                 710                 715             


<210>  10
<211>  486
<212>  PRT
<213>  Panax ginseng

<400>  10

Met Ala Ala Ala Met Val Leu Phe Phe Ser Leu Ser Leu Leu Leu Leu 
1               5                   10                  15      


Pro Leu Leu Leu Leu Phe Ala Tyr Phe Ser Tyr Thr Lys Arg Ile Pro 
            20                  25                  30          


Gln Lys Glu Asn Asp Ser Lys Ala Pro Leu Pro Pro Gly Gln Thr Gly 
        35                  40                  45              


Trp Pro Leu Ile Gly Glu Thr Leu Asn Tyr Leu Ser Cys Val Lys Ser 
    50                  55                  60                  


Gly Val Ser Glu Asn Phe Val Lys Tyr Arg Lys Glu Lys Tyr Ser Pro 
65                  70                  75                  80  


Lys Val Phe Arg Thr Ser Leu Leu Gly Glu Pro Met Ala Ile Leu Cys 
                85                  90                  95      


Gly Pro Glu Gly Asn Lys Phe Leu Tyr Ser Thr Glu Lys Lys Leu Val 
            100                 105                 110         


Gln Val Trp Phe Pro Ser Ser Val Glu Lys Met Phe Pro Arg Ser His 
        115                 120                 125             


Gly Glu Ser Asn Ala Asp Asn Phe Ser Lys Val Arg Gly Lys Met Met 
    130                 135                 140                 


Phe Leu Leu Lys Val Asp Gly Met Lys Lys Tyr Val Gly Leu Met Asp 
145                 150                 155                 160 


Arg Val Met Lys Gln Phe Leu Glu Thr Asp Trp Asn Arg Gln Gln Gln 
                165                 170                 175     


Ile Asn Val His Asn Thr Val Lys Lys Tyr Thr Val Thr Met Ser Cys 
            180                 185                 190         


Arg Val Phe Met Ser Ile Asp Asp Glu Glu Gln Val Thr Arg Leu Gly 
        195                 200                 205             


Ser Ser Ile Gln Asn Ile Glu Ala Gly Leu Leu Ala Val Pro Ile Asn 
    210                 215                 220                 


Ile Pro Gly Thr Ala Met Asn Arg Ala Ile Lys Thr Val Lys Leu Leu 
225                 230                 235                 240 


Thr Arg Glu Val Glu Ala Val Ile Lys Gln Arg Lys Val Asp Leu Leu 
                245                 250                 255     


Glu Asn Lys Gln Ala Ser Gln Pro Gln Asp Leu Leu Ser His Leu Leu 
            260                 265                 270         


Leu Thr Ala Asn Gln Asp Gly Gln Phe Leu Ser Glu Ser Asp Ile Ala 
        275                 280                 285             


Ser His Leu Ile Gly Leu Met Gln Gly Gly Tyr Thr Thr Leu Asn Gly 
    290                 295                 300                 


Thr Ile Thr Phe Val Leu Asn Tyr Leu Ala Glu Phe Pro Asp Val Tyr 
305                 310                 315                 320 


Asn Gln Val Leu Lys Glu Gln Val Glu Ile Ala Asn Ser Lys His Pro 
                325                 330                 335     


Lys Glu Leu Leu Asn Trp Glu Asp Leu Arg Lys Met Lys Tyr Ser Trp 
            340                 345                 350         


Asn Val Ala Gln Glu Val Leu Arg Ile Ile Pro Pro Gly Val Gly Thr 
        355                 360                 365             


Phe Arg Glu Ala Ile Thr Asp Phe Thr Tyr Ala Gly Tyr Leu Ile Pro 
    370                 375                 380                 


Lys Gly Trp Lys Met His Leu Ile Pro His Asp Thr His Lys Asn Pro 
385                 390                 395                 400 


Thr Tyr Phe Pro Ser Pro Glu Lys Phe Asp Pro Thr Arg Phe Glu Gly 
                405                 410                 415     


Asn Gly Pro Ala Pro Tyr Thr Phe Thr Pro Phe Gly Gly Gly Pro Arg 
            420                 425                 430         


Met Cys Pro Gly Ile Glu Tyr Ala Arg Leu Val Ile Leu Ile Phe Met 
        435                 440                 445             


His Asn Val Val Thr Asn Phe Arg Trp Glu Lys Leu Ile Pro Asn Glu 
    450                 455                 460                 


Lys Ile Leu Thr Asp Pro Ile Pro Arg Phe Ala His Gly Leu Pro Ile 
465                 470                 475                 480 


His Leu His Pro His Asn 
                485     


<210>  11
<211>  457
<212>  PRT
<213>  Panax ginseng

<400>  11

Met Glu Arg Glu Met Leu Ser Lys Thr His Ile Met Phe Ile Pro Phe 
1               5                   10                  15      


Pro Ala Gln Gly His Met Ser Pro Met Met Gln Phe Ala Lys Arg Leu 
            20                  25                  30          


Ala Trp Lys Gly Leu Arg Ile Thr Ile Val Leu Pro Ala Gln Ile Arg 
        35                  40                  45              


Asp Phe Met Gln Ile Thr Asn Pro Leu Ile Asn Thr Glu Cys Ile Ser 
    50                  55                  60                  


Phe Asp Phe Asp Lys Asp Asp Gly Met Pro Tyr Ser Met Gln Ala Tyr 
65                  70                  75                  80  


Met Gly Val Val Lys Leu Lys Val Thr Asn Lys Leu Ser Asp Leu Leu 
                85                  90                  95      


Glu Lys Gln Arg Thr Asn Gly Tyr Pro Val Asn Leu Leu Val Val Asp 
            100                 105                 110         


Ser Leu Tyr Pro Ser Arg Val Glu Met Cys His Gln Leu Gly Val Lys 
        115                 120                 125             


Gly Ala Pro Phe Phe Thr His Ser Cys Ala Val Gly Ala Ile Tyr Tyr 
    130                 135                 140                 


Asn Ala Arg Leu Gly Lys Leu Lys Ile Pro Pro Glu Glu Gly Leu Thr 
145                 150                 155                 160 


Ser Val Ser Leu Pro Ser Ile Pro Leu Leu Gly Arg Asp Asp Leu Pro 
                165                 170                 175     


Ile Ile Arg Thr Gly Thr Phe Pro Asp Leu Phe Glu His Leu Gly Asn 
            180                 185                 190         


Gln Phe Ser Asp Leu Asp Lys Ala Asp Trp Ile Phe Phe Asn Thr Phe 
        195                 200                 205             


Asp Lys Leu Glu Asn Glu Glu Ala Lys Trp Leu Ser Ser Gln Trp Pro 
    210                 215                 220                 


Ile Thr Ser Ile Gly Pro Leu Ile Pro Ser Met Tyr Leu Asp Lys Gln 
225                 230                 235                 240 


Leu Pro Asn Asp Lys Asp Asn Gly Ile Asn Phe Tyr Lys Ala Asp Val 
                245                 250                 255     


Gly Ser Cys Ile Lys Trp Leu Asp Ala Lys Asp Pro Gly Ser Val Val 
            260                 265                 270         


Tyr Ala Ser Phe Gly Ser Val Lys His Asn Leu Gly Asp Asp Tyr Met 
        275                 280                 285             


Asp Glu Val Ala Trp Gly Leu Leu His Ser Lys Tyr His Phe Ile Trp 
    290                 295                 300                 


Val Val Ile Glu Ser Glu Arg Thr Lys Leu Ser Ser Asp Phe Leu Ala 
305                 310                 315                 320 


Glu Ala Glu Ala Glu Glu Lys Gly Leu Ile Val Ser Trp Cys Pro Gln 
                325                 330                 335     


Leu Gln Val Leu Ser His Lys Ser Ile Gly Ser Phe Met Thr His Cys 
            340                 345                 350         


Gly Trp Asn Ser Thr Val Glu Ala Leu Ser Leu Gly Val Pro Met Val 
        355                 360                 365             


Ala Leu Pro Gln Gln Phe Asp Gln Pro Ala Asn Ala Lys Tyr Ile Val 
    370                 375                 380                 


Asp Val Trp Gln Ile Gly Val Arg Val Pro Ile Gly Glu Glu Gly Val 
385                 390                 395                 400 


Val Leu Arg Gly Glu Val Ala Asn Cys Ile Lys Asp Val Met Glu Gly 
                405                 410                 415     


Glu Ile Gly Asp Glu Leu Arg Gly Asn Ala Leu Lys Trp Lys Gly Leu 
            420                 425                 430         


Ala Val Glu Ala Met Glu Lys Gly Gly Ser Ser Asp Lys Asn Ile Asp 
        435                 440                 445             


Glu Phe Ile Ser Lys Leu Val Ser Ser 
    450                 455         


<210>  12
<211>  711
<212>  PRT
<213>  Arabidopsis thaliana

<400>  12

Met Ser Ser Ser Ser Ser Ser Ser Thr Ser Met Ile Asp Leu Met Ala 
1               5                   10                  15      


Ala Ile Ile Lys Gly Glu Pro Val Ile Val Ser Asp Pro Ala Asn Ala 
            20                  25                  30          


Ser Ala Tyr Glu Ser Val Ala Ala Glu Leu Ser Ser Met Leu Ile Glu 
        35                  40                  45              


Asn Arg Gln Phe Ala Met Ile Val Thr Thr Ser Ile Ala Val Leu Ile 
    50                  55                  60                  


Gly Cys Ile Val Met Leu Val Trp Arg Arg Ser Gly Ser Gly Asn Ser 
65                  70                  75                  80  


Lys Arg Val Glu Pro Leu Lys Pro Leu Val Ile Lys Pro Arg Glu Glu 
                85                  90                  95      


Glu Ile Asp Asp Gly Arg Lys Lys Val Thr Ile Phe Phe Gly Thr Gln 
            100                 105                 110         


Thr Gly Thr Ala Glu Gly Phe Ala Lys Ala Leu Gly Glu Glu Ala Lys 
        115                 120                 125             


Ala Arg Tyr Glu Lys Thr Arg Phe Lys Ile Val Asp Leu Asp Asp Tyr 
    130                 135                 140                 


Ala Ala Asp Asp Asp Glu Tyr Glu Glu Lys Leu Lys Lys Glu Asp Val 
145                 150                 155                 160 


Ala Phe Phe Phe Leu Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn 
                165                 170                 175     


Ala Ala Arg Phe Tyr Lys Trp Phe Thr Glu Gly Asn Asp Arg Gly Glu 
            180                 185                 190         


Trp Leu Lys Asn Leu Lys Tyr Gly Val Phe Gly Leu Gly Asn Arg Gln 
        195                 200                 205             


Tyr Glu His Phe Asn Lys Val Ala Lys Val Val Asp Asp Ile Leu Val 
    210                 215                 220                 


Glu Gln Gly Ala Gln Arg Leu Val Gln Val Gly Leu Gly Asp Asp Asp 
225                 230                 235                 240 


Gln Cys Ile Glu Asp Asp Phe Thr Ala Trp Arg Glu Ala Leu Trp Pro 
                245                 250                 255     


Glu Leu Asp Thr Ile Leu Arg Glu Glu Gly Asp Thr Ala Val Ala Thr 
            260                 265                 270         


Pro Tyr Thr Ala Ala Val Leu Glu Tyr Arg Val Ser Ile His Asp Ser 
        275                 280                 285             


Glu Asp Ala Lys Phe Asn Asp Ile Asn Met Ala Asn Gly Asn Gly Tyr 
    290                 295                 300                 


Thr Val Phe Asp Ala Gln His Pro Tyr Lys Ala Asn Val Ala Val Lys 
305                 310                 315                 320 


Arg Glu Leu His Thr Pro Glu Ser Asp Arg Ser Cys Ile His Leu Glu 
                325                 330                 335     


Phe Asp Ile Ala Gly Ser Gly Leu Thr Tyr Glu Thr Gly Asp His Val 
            340                 345                 350         


Gly Val Leu Cys Asp Asn Leu Ser Glu Thr Val Asp Glu Ala Leu Arg 
        355                 360                 365             


Leu Leu Asp Met Ser Pro Asp Thr Tyr Phe Ser Leu His Ala Glu Lys 
    370                 375                 380                 


Glu Asp Gly Thr Pro Ile Ser Ser Ser Leu Pro Pro Pro Phe Pro Pro 
385                 390                 395                 400 


Cys Asn Leu Arg Thr Ala Leu Thr Arg Tyr Ala Cys Leu Leu Ser Ser 
                405                 410                 415     


Pro Lys Lys Ser Ala Leu Val Ala Leu Ala Ala His Ala Ser Asp Pro 
            420                 425                 430         


Thr Glu Ala Glu Arg Leu Lys His Leu Ala Ser Pro Ala Gly Lys Asp 
        435                 440                 445             


Glu Tyr Ser Lys Trp Val Val Glu Ser Gln Arg Ser Leu Leu Glu Val 
    450                 455                 460                 


Met Ala Glu Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala 
465                 470                 475                 480 


Gly Val Ala Pro Arg Leu Gln Pro Arg Phe Tyr Ser Ile Ser Ser Ser 
                485                 490                 495     


Pro Lys Ile Ala Glu Thr Arg Ile His Val Thr Cys Ala Leu Val Tyr 
            500                 505                 510         


Glu Lys Met Pro Thr Gly Arg Ile His Lys Gly Val Cys Ser Thr Trp 
        515                 520                 525             


Met Lys Asn Ala Val Pro Tyr Glu Lys Ser Glu Asn Cys Ser Ser Ala 
    530                 535                 540                 


Pro Ile Phe Val Arg Gln Ser Asn Phe Lys Leu Pro Ser Asp Ser Lys 
545                 550                 555                 560 


Val Pro Ile Ile Met Ile Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg 
                565                 570                 575     


Gly Phe Leu Gln Glu Arg Leu Ala Leu Val Glu Ser Gly Val Glu Leu 
            580                 585                 590         


Gly Pro Ser Val Leu Phe Phe Gly Cys Arg Asn Arg Arg Met Asp Phe 
        595                 600                 605             


Ile Tyr Glu Glu Glu Leu Gln Arg Phe Val Glu Ser Gly Ala Leu Ala 
    610                 615                 620                 


Glu Leu Ser Val Ala Phe Ser Arg Glu Gly Pro Thr Lys Glu Tyr Val 
625                 630                 635                 640 


Gln His Lys Met Met Asp Lys Ala Ser Asp Ile Trp Asn Met Ile Ser 
                645                 650                 655     


Gln Gly Ala Tyr Leu Tyr Val Cys Gly Asp Ala Lys Gly Met Ala Arg 
            660                 665                 670         


Asp Val His Arg Ser Leu His Thr Ile Ala Gln Glu Gln Gly Ser Met 
        675                 680                 685             


Asp Ser Thr Lys Ala Glu Gly Phe Val Lys Asn Leu Gln Thr Ser Gly 
    690                 695                 700                 


Arg Tyr Leu Arg Asp Val Trp 
705                 710     


<210>  13
<211>  769
<212>  PRT
<213>  Panax ginseng

<400>  13

Met Trp Lys Gln Lys Gly Ala Gln Gly Asn Asp Pro Tyr Leu Tyr Ser 
1               5                   10                  15      


Thr Asn Asn Phe Val Gly Arg Gln Tyr Trp Glu Phe Gln Pro Asp Ala 
            20                  25                  30          


Gly Thr Pro Glu Glu Arg Glu Glu Val Glu Lys Ala Arg Lys Asp Tyr 
        35                  40                  45              


Val Asn Asn Lys Lys Leu His Gly Ile His Pro Cys Ser Asp Met Leu 
    50                  55                  60                  


Met Arg Arg Gln Leu Ile Lys Glu Ser Gly Ile Asp Leu Leu Ser Ile 
65                  70                  75                  80  


Pro Pro Leu Arg Leu Asp Glu Asn Glu Gln Val Asn Tyr Asp Ala Val 
                85                  90                  95      


Thr Thr Ala Val Lys Lys Ala Leu Arg Leu Asn Arg Ala Ile Gln Ala 
            100                 105                 110         


His Asp Gly His Trp Pro Ala Glu Asn Ala Gly Ser Leu Leu Tyr Thr 
        115                 120                 125             


Pro Pro Leu Ile Ile Ala Leu Tyr Ile Ser Gly Thr Ile Asp Thr Ile 
    130                 135                 140                 


Leu Thr Lys Gln His Lys Lys Glu Leu Ile Arg Phe Val Tyr Asn His 
145                 150                 155                 160 


Gln Asn Glu Asp Gly Gly Trp Gly Ser Tyr Ile Glu Gly His Ser Thr 
                165                 170                 175     


Met Ile Gly Ser Val Leu Ser Tyr Val Met Leu Arg Leu Leu Gly Glu 
            180                 185                 190         


Gly Leu Ala Glu Ser Asp Asp Gly Asn Gly Ala Val Glu Arg Gly Arg 
        195                 200                 205             


Lys Trp Ile Leu Asp His Gly Gly Ala Ala Gly Ile Pro Ser Trp Gly 
    210                 215                 220                 


Lys Thr Tyr Leu Ala Val Leu Gly Val Tyr Glu Trp Glu Gly Cys Asn 
225                 230                 235                 240 


Pro Leu Pro Pro Glu Phe Trp Leu Phe Pro Ser Ser Phe Pro Phe His 
                245                 250                 255     


Pro Ala Lys Met Trp Ile Tyr Cys Arg Cys Thr Tyr Met Pro Met Ser 
            260                 265                 270         


Tyr Leu Tyr Gly Lys Arg Tyr His Gly Pro Ile Thr Asp Leu Val Leu 
        275                 280                 285             


Ser Leu Arg Gln Glu Ile Tyr Asn Ile Pro Tyr Glu Gln Ile Lys Trp 
    290                 295                 300                 


Asn Gln Gln Arg His Asn Cys Cys Lys Glu Asp Leu Tyr Tyr Pro His 
305                 310                 315                 320 


Thr Leu Val Gln Asp Leu Val Trp Asp Gly Leu His Tyr Phe Ser Glu 
                325                 330                 335     


Pro Phe Leu Lys Arg Trp Pro Phe Asn Lys Leu Arg Lys Arg Gly Leu 
            340                 345                 350         


Lys Arg Val Val Glu Leu Met Arg Tyr Gly Ala Thr Glu Thr Arg Phe 
        355                 360                 365             


Ile Thr Thr Gly Asn Gly Glu Lys Ala Leu Gln Ile Met Ser Trp Trp 
    370                 375                 380                 


Ala Glu Asp Pro Asn Gly Asp Glu Phe Lys His His Leu Ala Arg Ile 
385                 390                 395                 400 


Pro Asp Phe Leu Trp Ile Ala Glu Asp Gly Met Thr Val Gln Ser Phe 
                405                 410                 415     


Gly Ser Gln Leu Trp Asp Cys Ile Leu Ala Thr Gln Ala Ile Ile Ala 
            420                 425                 430         


Thr Asn Met Val Glu Glu Tyr Gly Asp Ser Leu Lys Lys Ala His Phe 
        435                 440                 445             


Phe Ile Lys Glu Ser Gln Ile Lys Glu Asn Pro Arg Gly Asp Phe Leu 
    450                 455                 460                 


Lys Met Cys Arg Gln Phe Thr Lys Gly Ala Trp Thr Phe Ser Asp Gln 
465                 470                 475                 480 


Asp His Gly Cys Val Val Ser Asp Cys Thr Ala Glu Ala Leu Lys Cys 
                485                 490                 495     


Leu Leu Leu Leu Ser Gln Met Pro Gln Asp Ile Val Gly Glu Lys Pro 
            500                 505                 510         


Glu Val Glu Arg Leu Tyr Glu Ala Val Asn Val Leu Leu Tyr Leu Gln 
        515                 520                 525             


Ser Arg Val Ser Gly Gly Phe Ala Val Trp Glu Pro Pro Val Pro Lys 
    530                 535                 540                 


Pro Tyr Leu Glu Met Leu Asn Pro Ser Glu Ile Phe Ala Asp Ile Val 
545                 550                 555                 560 


Val Glu Arg Glu His Ile Glu Cys Thr Ala Ser Val Ile Lys Gly Leu 
                565                 570                 575     


Met Ala Phe Lys Cys Leu His Pro Gly His Arg Gln Lys Glu Ile Glu 
            580                 585                 590         


Asp Ser Val Ala Lys Ala Ile Arg Tyr Leu Glu Arg Asn Gln Met Pro 
        595                 600                 605             


Asp Gly Ser Trp Tyr Gly Phe Trp Gly Ile Cys Phe Leu Tyr Gly Thr 
    610                 615                 620                 


Phe Phe Thr Leu Ser Gly Phe Ala Ser Ala Gly Arg Thr Tyr Asp Asn 
625                 630                 635                 640 


Ser Glu Ala Val Arg Lys Gly Val Lys Phe Phe Leu Ser Thr Gln Asn 
                645                 650                 655     


Glu Glu Gly Gly Trp Gly Glu Ser Leu Glu Ser Cys Pro Ser Glu Lys 
            660                 665                 670         


Phe Thr Pro Leu Lys Gly Asn Arg Thr Asn Leu Val Gln Thr Ser Trp 
        675                 680                 685             


Ala Met Leu Gly Leu Met Phe Gly Gly Gln Ala Glu Arg Asp Pro Thr 
    690                 695                 700                 


Pro Leu His Arg Ala Ala Lys Leu Leu Ile Asn Ala Gln Met Asp Asn 
705                 710                 715                 720 


Gly Asp Phe Pro Gln Gln Glu Ile Thr Gly Val Tyr Cys Lys Asn Ser 
                725                 730                 735     


Met Leu His Tyr Ala Glu Tyr Arg Asn Ile Phe Pro Leu Trp Ala Leu 
            740                 745                 750         


Gly Glu Tyr Arg Lys Arg Val Trp Leu Pro Lys His Gln Gln Leu Lys 
        755                 760                 765             


Ile 
    


<210>  14
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  consensus NFAT recognition sequence

<400>  14
ggaaaa                                                                   6


<210>  15
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-zeta endodomain

<400>  15

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  16
<211>  368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB and CD3-zeta endodomains

<400>  16

Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu 
1               5                   10                  15      


Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro 
            20                  25                  30          


Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys 
        35                  40                  45              


Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile 
    50                  55                  60                  


Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser 
65                  70                  75                  80  


Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly 
                85                  90                  95      


Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu 
            100                 105                 110         


Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln 
        115                 120                 125             


Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys 
    130                 135                 140                 


Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro 
145                 150                 155                 160 


Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala 
                165                 170                 175     


Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu 
            180                 185                 190         


Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu 
        195                 200                 205             


Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
    210                 215                 220                 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
225                 230                 235                 240 


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
                245                 250                 255     


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
            260                 265                 270         


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
        275                 280                 285             


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
    290                 295                 300                 


Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
305                 310                 315                 320 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
                325                 330                 335     


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
            340                 345                 350         


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        355                 360                 365             


<210>  17
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 and CD3-zeta endodomains

<400>  17

Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
1               5                   10                  15      


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            20                  25                  30          


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
        35                  40                  45              


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    50                  55                  60                  


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
65                  70                  75                  80  


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                85                  90                  95      


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            100                 105                 110         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        115                 120                 125             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    130                 135                 140                 


His Met Gln Ala Leu Pro Pro Arg 
145                 150         


<210>  18
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28, OX40 and CD3-zeta endodomains

<400>  18

Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
1               5                   10                  15      


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            20                  25                  30          


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp 
        35                  40                  45              


Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu 
    50                  55                  60                  


Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe 
65                  70                  75                  80  


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
                85                  90                  95      


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
            100                 105                 110         


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
        115                 120                 125             


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
    130                 135                 140                 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
145                 150                 155                 160 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
                165                 170                 175     


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            180                 185             


<210>  19
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  basic amino acid furin target sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Arg or Lys

<400>  19

Arg Xaa Xaa Arg 
1               


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  20

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  21
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM (immunoreceptor tyrosine-based activation motif)


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa may be Leu or Ile

<400>  21

Tyr Xaa Xaa Xaa 
1               


