                         SEQUENCE LISTING

<110>  Navigo Proteins GmbH
 
<120>  Fusion proteins with specificity for extradomain B of fibronectin
        (ED-B) and improved serum half-life for diagnosis or treatment 
       of cancer

<130>  SP34 WO

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-77404

<400>  1

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  2
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800

<400>  2

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150             


<210>  3
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138801

<400>  3

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asn Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Ile 
65                  70                  75                  80  


Gln Thr Phe Val Thr Thr Gln Thr Gly Glu Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Val Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Tyr 
    130                 135                 140                 


Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 


<210>  4
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-102472

<400>  4

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Tyr 
    130                 135                 140                 


Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 


<210>  5
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 Y141A

<400>  5

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Ala Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150             


<210>  6
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 RAGL

<400>  6

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Gly Leu Ala 
145                 150             


<210>  7
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 RAAL

<400>  7

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Ala Leu Ala 
145                 150             


<210>  8
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 2274-4

<400>  8

Met Gln Ile Phe Val His Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Trp Val 
    50                  55                  60                  


Arg Gln Leu His Leu Val Leu Arg Leu Arg Ala Ala Met Gln Ile Phe 
65                  70                  75                  80  


Val Leu Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Trp Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala 
145                 150     


<210>  9
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 2274-42

<400>  9

Met Gln Ile Phe Val His Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Trp Val 
    50                  55                  60                  


Arg Gln Leu His Leu Val Leu Arg Leu Arg Ala Ala Ile Gln Ile Phe 
65                  70                  75                  80  


Val Leu Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Trp Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala 
145                 150     


<210>  10
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 2274-43

<400>  10

Met Gln Ile Phe Val His Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Trp Val 
    50                  55                  60                  


Arg Gln Leu His Leu Val Leu Arg Leu Arg Ala Ala Met Gln Ile Phe 
65                  70                  75                  80  


Val Leu Thr Trp Thr Gly Lys Thr Ile Thr Leu Gly Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Trp Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala 
145                 150     


<210>  11
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 2273-31

<400>  11

Met Gln Ile Phe Val His Thr Thr Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Gly Val 
    50                  55                  60                  


Arg Met Leu His Leu Val Leu Arg Leu Arg Ala Ala Met Gln Ile Phe 
65                  70                  75                  80  


Val Met Thr Arg Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Trp Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala 
145                 150     


<210>  12
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 2273-32

<400>  12

Met Gln Ile Phe Val His Thr Arg Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Ala His Gln 
    50                  55                  60                  


Pro Gln Leu His Leu Val Leu Arg Leu Arg Ala Ala Met Gln Ile Phe 
65                  70                  75                  80  


Val Gln Thr Met Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Tyr Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala 
145                 150     


<210>  13
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-181494 (del 62/63)

<400>  13

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Lys 
    50                  55                  60                  


Ser His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe 
65                  70                  75                  80  


Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala 
145                 150     


<210>  14
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APS domain

<400>  14

Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro 
1               5                   10                  15      


Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala 
            20                  25                  30          


Ser Ala Pro Ala Ala Ala Ser Ala 
        35                  40  


<210>  15
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APS domain with cys

<400>  15

Ser Ala Pro Ala Ala Ser Pro Ser Ala Ala Ala Pro Ala Pro Ser Pro 
1               5                   10                  15      


Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala 
            20                  25                  30          


Ser Ala Pro Pro Ala Ala Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser 
        35                  40                  45              


Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala 
    50                  55                  60  


<210>  16
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APS domain with Asp

<400>  16

Ser Ala Pro Ala Ala Ser Pro Asp Pro Ala Ala Pro Ala Pro Ser Asp 
1               5                   10                  15      


Ala Ser Pro Ala Pro Ala Ser Asp Ala Ser Ala Pro Ser Ala Pro Asp 
            20                  25                  30          


Ser Ala Pro Ala Ala Ala Ser Ala 
        35                  40  


<210>  17
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APS domain with Asp and Cys

<400>  17

Ser Ala Pro Ala Ala Asp Pro Ser Ala Ala Ala Asp Ala Pro Ser Pro 
1               5                   10                  15      


Ala Asp Pro Ala Pro Ala Ser Asp Ala Ser Ala Pro Ser Asp Pro Ala 
            20                  25                  30          


Ser Ala Asp Pro Ala Ala Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser 
        35                  40                  45              


Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala 
    50                  55                  60  


<210>  18
<211>  79
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APS domain

<400>  18

Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro 
1               5                   10                  15      


Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala 
            20                  25                  30          


Ser Ala Pro Ala Ala Ala Ser Ala Ser Ala Pro Ala Ala Ser Pro Ser 
        35                  40                  45              


Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro 
    50                  55                  60                  


Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala Ala Ser Ala 
65                  70                  75                  


<210>  19
<211>  79
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APS domain with Cys

<400>  19

Ser Ala Pro Ala Ala Ser Pro Ser Ala Ala Ala Pro Ala Pro Ser Pro 
1               5                   10                  15      


Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala 
            20                  25                  30          


Ser Ala Pro Pro Ala Ala Ser Pro Ser Ala Pro Ala Ala Ser Pro Ser 
        35                  40                  45              


Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Cys Ala Ala Ala Ser Pro 
    50                  55                  60                  


Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser 
65                  70                  75                  


<210>  20
<211>  79
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APS domain

<400>  20

Ser Ala Pro Ala Ala Ser Pro Ser Ala Ala Ala Pro Ala Pro Ser Pro 
1               5                   10                  15      


Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala 
            20                  25                  30          


Ser Ala Pro Pro Ala Ala Ser Pro Ser Ala Pro Ala Ala Ser Pro Ser 
        35                  40                  45              


Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Ala Ala Ser Pro 
    50                  55                  60                  


Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser 
65                  70                  75                  


<210>  21
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-138800 / APS domain

<400>  21

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro 
145                 150                 155                 160 


Ser Ala Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser 
                165                 170                 175     


Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Pro Ala Ala Ser 
            180                 185                 190         


Cys Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser 
        195                 200                 205             


Pro Ala Ser Pro Ala 
    210             


<210>  22
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-138800 / APS domain with Asp

<400>  22

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Asp Pro 
145                 150                 155                 160 


Ser Ala Ala Ala Asp Ala Pro Ser Pro Ala Asp Pro Ala Pro Ala Ser 
                165                 170                 175     


Asp Ala Ser Ala Pro Ser Asp Pro Ala Ser Ala Asp Pro Ala Ala Ser 
            180                 185                 190         


Cys Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser 
        195                 200                 205             


Pro Ala Ser Pro Ala 
    210             


<210>  23
<211>  406
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-138800 / APS domain / Affilin-138800 / APS
       domain with Cys

<400>  23

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro 
145                 150                 155                 160 


Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser 
                165                 170                 175     


Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala Ala Ala Ser 
            180                 185                 190         


Ala Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu 
        195                 200                 205             


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln 
    210                 215                 220                 


Asp Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly 
225                 230                 235                 240 


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro 
                245                 250                 255     


Gln Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met 
            260                 265                 270         


Gln Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
        275                 280                 285             


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
    290                 295                 300                 


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
305                 310                 315                 320 


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe 
                325                 330                 335     


Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser 
            340                 345                 350         


Pro Ser Ala Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala 
        355                 360                 365             


Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Pro Ala Ala 
    370                 375                 380                 


Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro 
385                 390                 395                 400 


Ser Pro Ala Ser Pro Ala 
                405     


<210>  24
<211>  406
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-138800 / APS domain with Asp / 
       Affilin-138800 / APS domain with Asp and Cys

<400>  24

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro 
145                 150                 155                 160 


Asp Pro Ala Ala Pro Ala Pro Ser Asp Ala Ser Pro Ala Pro Ala Ser 
                165                 170                 175     


Asp Ala Ser Ala Pro Ser Ala Pro Asp Ser Ala Pro Ala Ala Ala Ser 
            180                 185                 190         


Ala Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu 
        195                 200                 205             


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln 
    210                 215                 220                 


Asp Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly 
225                 230                 235                 240 


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro 
                245                 250                 255     


Gln Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met 
            260                 265                 270         


Gln Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
        275                 280                 285             


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
    290                 295                 300                 


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
305                 310                 315                 320 


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe 
                325                 330                 335     


Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Asp 
            340                 345                 350         


Pro Ser Ala Ala Ala Asp Ala Pro Ser Pro Ala Asp Pro Ala Pro Ala 
        355                 360                 365             


Ser Asp Ala Ser Ala Pro Ser Asp Pro Ala Ser Ala Asp Pro Ala Ala 
    370                 375                 380                 


Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro 
385                 390                 395                 400 


Ser Pro Ala Ser Pro Ala 
                405     


<210>  25
<211>  464
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-138800 / APS domain  / Affilin-138800 / 
       APS domain with Cys

<400>  25

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro 
145                 150                 155                 160 


Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser 
                165                 170                 175     


Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala Ala Ala Ser 
            180                 185                 190         


Ala Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser 
        195                 200                 205             


Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro 
    210                 215                 220                 


Ala Ser Ala Pro Ala Ala Ser Ala Met Gln Ile Phe Val His Thr Asp 
225                 230                 235                 240 


Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu 
                245                 250                 255     


Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Gln Asp Gln 
            260                 265                 270         


Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu 
        275                 280                 285             


Ser Asp Tyr Asn Ile Asp Pro Gln Leu Lys Leu His Leu Val Leu Arg 
    290                 295                 300                 


Leu Arg Ala Ala Gly Gly Met Gln Ile Phe Val Thr Thr Gln Thr Gly 
305                 310                 315                 320 


Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val 
                325                 330                 335     


Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg 
            340                 345                 350         


Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp 
        355                 360                 365             


Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu Val Leu Arg Leu Arg Ala 
    370                 375                 380                 


Ala Ser Ala Pro Ala Ala Ser Pro Ser Ala Ala Ala Pro Ala Pro Ser 
385                 390                 395                 400 


Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro 
                405                 410                 415     


Ala Ser Ala Pro Pro Ala Ala Ser Pro Ser Ala Pro Ala Ala Ser Pro 
            420                 425                 430         


Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Ala Ala Ser 
        435                 440                 445             


Pro Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser 
    450                 455                 460                 


<210>  26
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-77404 / APS domain with Cys

<400>  26

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala 
145                 150                 155                 160 


Ser Pro Ser Ala Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro 
                165                 170                 175     


Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Pro Ala 
            180                 185                 190         


Ala Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala 
        195                 200                 205             


Pro Ser Pro Ala Ser Pro Ala 
    210                 215 


<210>  27
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-77404 / APS domain with Asp and Cys

<400>  27

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala 
145                 150                 155                 160 


Asp Pro Ser Ala Ala Ala Asp Ala Pro Ser Pro Ala Asp Pro Ala Pro 
                165                 170                 175     


Ala Ser Asp Ala Ser Ala Pro Ser Asp Pro Ala Ser Ala Asp Pro Ala 
            180                 185                 190         


Ala Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala 
        195                 200                 205             


Pro Ser Pro Ala Ser Pro Ala 
    210                 215 


<210>  28
<211>  410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-77404 / APS domain / Affilin-77404 / APS 
       domain with Cys

<400>  28

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala 
145                 150                 155                 160 


Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro 
                165                 170                 175     


Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala Ala 
            180                 185                 190         


Ala Ser Ala Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile 
        195                 200                 205             


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
    210                 215                 220                 


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp 
225                 230                 235                 240 


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
                245                 250                 255     


Asn Pro Lys Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly 
            260                 265                 270         


Ile Gly Met Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr 
        275                 280                 285             


Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile 
    290                 295                 300                 


Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala 
305                 310                 315                 320 


Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly 
                325                 330                 335     


Trp Gln Ala Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala 
            340                 345                 350         


Pro Ala Ala Ser Pro Ser Ala Ala Ala Pro Ala Pro Ser Pro Ala Ser 
        355                 360                 365             


Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala 
    370                 375                 380                 


Pro Pro Ala Ala Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala 
385                 390                 395                 400 


Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala 
                405                 410 


<210>  29
<211>  410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-77404 / APS domain with Asp / 
       Affilin-77404 / APS domain with Asp and Cys

<400>  29

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala 
145                 150                 155                 160 


Ser Pro Asp Pro Ala Ala Pro Ala Pro Ser Asp Ala Ser Pro Ala Pro 
                165                 170                 175     


Ala Ser Asp Ala Ser Ala Pro Ser Ala Pro Asp Ser Ala Pro Ala Ala 
            180                 185                 190         


Ala Ser Ala Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile 
        195                 200                 205             


Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys 
    210                 215                 220                 


Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp 
225                 230                 235                 240 


Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile 
                245                 250                 255     


Asn Pro Lys Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly 
            260                 265                 270         


Ile Gly Met Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr 
        275                 280                 285             


Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile 
    290                 295                 300                 


Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala 
305                 310                 315                 320 


Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly 
                325                 330                 335     


Trp Gln Ala Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala 
            340                 345                 350         


Pro Ala Ala Asp Pro Ser Ala Ala Ala Asp Ala Pro Ser Pro Ala Asp 
        355                 360                 365             


Pro Ala Pro Ala Ser Asp Ala Ser Ala Pro Ser Asp Pro Ala Ser Ala 
    370                 375                 380                 


Asp Pro Ala Ala Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala 
385                 390                 395                 400 


Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala 
                405                 410 


<210>  30
<211>  468
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-77404 / APS domain with Asp / 
       Affilin-77404 / APS domain with Asp and Cys

<400>  30

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala 
145                 150                 155                 160 


Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro 
                165                 170                 175     


Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala Ala 
            180                 185                 190         


Ala Ser Ala Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala 
        195                 200                 205             


Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser 
    210                 215                 220                 


Ala Pro Ala Ser Ala Pro Ala Ala Ser Ala Met Arg Ile Trp Val His 
225                 230                 235                 240 


Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr 
                245                 250                 255     


Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro 
            260                 265                 270         


Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg 
        275                 280                 285             


Thr Leu Ser Asp Tyr Asn Ile Asn Pro Lys Leu Ser Leu His Leu Val 
    290                 295                 300                 


Leu Arg Leu Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val His Thr 
305                 310                 315                 320 


Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile 
                325                 330                 335     


Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp 
            340                 345                 350         


Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr 
        355                 360                 365             


Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala Pro Leu His Leu Val Leu 
    370                 375                 380                 


Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro Ser Ala Ala Ala 
385                 390                 395                 400 


Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala 
                405                 410                 415     


Pro Ser Ala Pro Ala Ser Ala Pro Pro Ala Ala Ser Pro Ser Ala Pro 
            420                 425                 430         


Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro 
        435                 440                 445             


Ala Ala Ala Ser Pro Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala 
    450                 455                 460                 


Pro Ala Pro Ser 
465             


<210>  31
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bis-ubiquitin (CID64156)

<400>  31

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 
    130                 135                 140                 


Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  32
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  bis-ubiquitin, wildtype

<400>  32

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser 
    130                 135                 140                 


Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
145                 150                 155 


<210>  33
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-1041D11

<400>  33

Met Gln Ile Phe Val Trp Thr Trp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Arg Lys 
    50                  55                  60                  


Phe Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Arg Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Trp Ser Asn Trp 
    130                 135                 140                 


Glu Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  34
<211>  155
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-77404 mit S57C

<400>  34

Met Arg Ile Trp Val His Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Cys Asp Tyr Asn Ile Asn Pro Lys 
    50                  55                  60                  


Leu Ser Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Ile Gly Met 
65                  70                  75                  80  


Gln Ile Phe Val His Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val 
                85                  90                  95      


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
            100                 105                 110         


Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
        115                 120                 125             


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gly Trp Gln Ala 
    130                 135                 140                 


Pro Leu His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150                 155 


<210>  35
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-138800 mit S57C

<400>  35

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Cys Asp Tyr Asn Ile Asp Pro Gln 
    50                  55                  60                  


Leu Lys Leu His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln 
65                  70                  75                  80  


Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
                85                  90                  95      


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
            100                 105                 110         


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu 
        115                 120                 125             


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu 
    130                 135                 140                 


His Leu Val Leu Arg Leu Arg Ala Ala 
145                 150             


<210>  36
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin-181494 with S57C (corresponding to pos 57 of SEQ ID NO 
       32)

<400>  36

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Cys Asp Tyr Asn Ile Gln Lys Lys 
    50                  55                  60                  


Ser His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe 
65                  70                  75                  80  


Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala 
145                 150     


<210>  37
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ubiquitin (monomer based on 64156)

<400>  37

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala 
65                  70                  75      


<210>  38
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ubiquitin wildtype

<400>  38

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  39
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin--181494 /APS domain

<400>  39

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Lys 
    50                  55                  60                  


Ser His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe 
65                  70                  75                  80  


Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro Ser Ala 
145                 150                 155                 160 


Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala 
                165                 170                 175     


Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Pro Ala Ala Ser Cys Ser 
            180                 185                 190         


Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala 
        195                 200                 205             


Ser Pro Ala 
    210     


<210>  40
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-181494 / APS domain with Asp;

<400>  40

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Lys 
    50                  55                  60                  


Ser His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe 
65                  70                  75                  80  


Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Asp Pro Ser Ala 
145                 150                 155                 160 


Ala Ala Asp Ala Pro Ser Pro Ala Asp Pro Ala Pro Ala Ser Asp Ala 
                165                 170                 175     


Ser Ala Pro Ser Asp Pro Ala Ser Ala Asp Pro Ala Ala Ser Cys Ser 
            180                 185                 190         


Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala 
        195                 200                 205             


Ser Pro Ala 
    210     


<210>  41
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin-181494 / APS domain / Affilin--181494/ APS
       domain with Cys

<400>  41

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Lys 
    50                  55                  60                  


Ser His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe 
65                  70                  75                  80  


Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro Ser Pro 
145                 150                 155                 160 


Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala 
                165                 170                 175     


Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala Ala Ala Ser Ala Met 
            180                 185                 190         


Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu Val 
        195                 200                 205             


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
    210                 215                 220                 


Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
225                 230                 235                 240 


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Lys Ser 
                245                 250                 255     


His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe Val 
            260                 265                 270         


Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp 
        275                 280                 285             


Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro 
    290                 295                 300                 


Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly 
305                 310                 315                 320 


Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu Val 
                325                 330                 335     


Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro Ser Ala Ala 
            340                 345                 350         


Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser 
        355                 360                 365             


Ala Pro Ser Ala Pro Ala Ser Ala Pro Pro Ala Ala Ser Cys Ser Ala 
    370                 375                 380                 


Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser 
385                 390                 395                 400 


Pro Ala 
        


<210>  42
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin--181494 / APS domain with Asp / 
       Affilin--181494 / APS domain with Asp and Cys

<400>  42

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Lys 
    50                  55                  60                  


Ser His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe 
65                  70                  75                  80  


Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro Asp Pro 
145                 150                 155                 160 


Ala Ala Pro Ala Pro Ser Asp Ala Ser Pro Ala Pro Ala Ser Asp Ala 
                165                 170                 175     


Ser Ala Pro Ser Ala Pro Asp Ser Ala Pro Ala Ala Ala Ser Ala Met 
            180                 185                 190         


Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu Val 
        195                 200                 205             


Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys 
    210                 215                 220                 


Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln 
225                 230                 235                 240 


Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Lys Ser 
                245                 250                 255     


His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe Val 
            260                 265                 270         


Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp 
        275                 280                 285             


Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro 
    290                 295                 300                 


Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly 
305                 310                 315                 320 


Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu Val 
                325                 330                 335     


Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Asp Pro Ser Ala Ala 
            340                 345                 350         


Ala Asp Ala Pro Ser Pro Ala Asp Pro Ala Pro Ala Ser Asp Ala Ser 
        355                 360                 365             


Ala Pro Ser Asp Pro Ala Ser Ala Asp Pro Ala Ala Ser Cys Ser Ala 
    370                 375                 380                 


Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser 
385                 390                 395                 400 


Pro Ala 
        


<210>  43
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein Affilin--181494/ APS domain  / Affilin-181494 / 
       APS domain with Cy

<400>  43

Met Gln Ile Phe Val His Thr Asp Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Lys 
    50                  55                  60                  


Ser His Leu Val Leu Arg Leu Arg Ala Ala Gly Gly Met Gln Ile Phe 
65                  70                  75                  80  


Val Thr Thr Gln Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Phe Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro Ser Pro 
145                 150                 155                 160 


Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala 
                165                 170                 175     


Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala Ala Ala Ser Ala Ser 
            180                 185                 190         


Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala 
        195                 200                 205             


Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser 
    210                 215                 220                 


Ala Pro Ala Ala Ser Ala Met Gln Ile Phe Val His Thr Asp Thr Gly 
225                 230                 235                 240 


Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val 
                245                 250                 255     


Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Gln Asp Gln Gln Arg 
            260                 265                 270         


Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp 
        275                 280                 285             


Tyr Asn Ile Gln Lys Lys Ser His Leu Val Leu Arg Leu Arg Ala Ala 
    290                 295                 300                 


Gly Gly Met Gln Ile Phe Val Thr Thr Gln Thr Gly Lys Thr Ile Thr 
305                 310                 315                 320 


Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile 
                325                 330                 335     


Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala 
            340                 345                 350         


Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp 
        355                 360                 365             


Tyr Arg Phe Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro 
    370                 375                 380                 


Ala Ala Ser Pro Ser Ala Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro 
385                 390                 395                 400 


Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro 
                405                 410                 415     


Pro Ala Ala Ser Pro Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala 
            420                 425                 430         


Pro Ala Pro Ser Pro Ala Ser Pro Ala Ala Ala Ser Pro Ser Ala Pro 
        435                 440                 445             


Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser 
    450                 455                 460 


<210>  44
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CID 180339 (UBI-40-Ubi-60)

<400>  44

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala 
65                  70                  75                  80  


Ala Ser Pro Ser Pro Ala Ala Pro Gln Pro Ser Pro Ala Ser Pro Ala 
                85                  90                  95      


Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala 
            100                 105                 110         


Ala Ala Ser Ala Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr 
        115                 120                 125             


Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala 
    130                 135                 140                 


Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile 
145                 150                 155                 160 


Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn 
                165                 170                 175     


Ile Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala 
            180                 185                 190         


Ser Ala Pro Ala Ala Ser Pro Ser Ala Ala Ala Pro Ala Pro Ser Pro 
        195                 200                 205             


Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala 
    210                 215                 220                 


Ser Ala Pro Pro Ala Ala Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser 
225                 230                 235                 240 


Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala 
                245                 250         


<210>  45
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CID 180340 (UBI-40APSD-UBI-60APSD/C)

<400>  45

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala 
65                  70                  75                  80  


Ala Ser Pro Asp Pro Ala Ala Pro Gln Pro Ser Asp Ala Ser Pro Ala 
                85                  90                  95      


Pro Ala Ser Asp Ala Ser Ala Pro Ser Ala Pro Asp Ser Ala Pro Ala 
            100                 105                 110         


Ala Ala Ser Ala Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr 
        115                 120                 125             


Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala 
    130                 135                 140                 


Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile 
145                 150                 155                 160 


Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn 
                165                 170                 175     


Ile Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala 
            180                 185                 190         


Ser Ala Pro Ala Ala Asp Pro Ser Ala Ala Ala Asp Ala Pro Ser Pro 
        195                 200                 205             


Ala Asp Pro Ala Pro Ala Ser Asp Ala Ser Ala Pro Ser Asp Pro Ala 
    210                 215                 220                 


Ser Ala Asp Pro Ala Ala Ser Cys Ser Ala Pro Ala Ala Ser Pro Ser 
225                 230                 235                 240 


Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala 
                245                 250         


<210>  46
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CID 180341 (UBI-79APS-UBI-79APS/C)

<400>  46

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala 
65                  70                  75                  80  


Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala 
                85                  90                  95      


Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala 
            100                 105                 110         


Ala Ala Ser Ala Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro 
        115                 120                 125             


Ala Pro Ser Pro Ala Ser Pro Gln Pro Ala Ser Pro Ala Ser Ala Pro 
    130                 135                 140                 


Ser Ala Pro Ala Ser Ala Pro Ala Ala Ser Ala Met Gln Ile Phe Val 
145                 150                 155                 160 


Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp 
                165                 170                 175     


Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro 
            180                 185                 190         


Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly 
        195                 200                 205             


Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His Leu 
    210                 215                 220                 


Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro Ser Ala 
225                 230                 235                 240 


Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala 
                245                 250                 255     


Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Pro Ala Ala Ser Pro Ser 
            260                 265                 270         


Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala 
        275                 280                 285             


Ser Cys Ala Ala Ala Ser Pro Ser Ala Pro Ala Ala Ser Pro Ser Pro 
    290                 295                 300                 


Ala Ala Pro Ala Pro Ser 
305                 310 


<210>  47
<211>  487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CID 181790 (79APS-UBI-79APS-UBI-79APS/C)

<400>  47

Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 
1               5                   10                  15      


Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 
            20                  25                  30          


Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 
        35                  40                  45              


Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 
    50                  55                  60                  


Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 
65                  70                  75                  80  


Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 
                85                  90                  95      


Gly Gly Ser Ala Ser Ala Ala Ser Pro Ser Pro Ala Ala Pro Gln Pro 
            100                 105                 110         


Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala 
        115                 120                 125             


Pro Ala Ser Ala Pro Ala Ala Ala Ser Ala Ser Ala Pro Ala Ala Ser 
    130                 135                 140                 


Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala 
145                 150                 155                 160 


Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Ala Ala Ser 
                165                 170                 175     


Ala Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu 
            180                 185                 190         


Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln 
        195                 200                 205             


Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly 
    210                 215                 220                 


Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys 
225                 230                 235                 240 


Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro 
                245                 250                 255     


Ala Ala Ser Pro Ser Ala Ala Ala Pro Gln Pro Ser Pro Ala Ser Pro 
            260                 265                 270         


Ala Pro Ala Ser Pro Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro 
        275                 280                 285             


Pro Ala Ala Ser Pro Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala 
    290                 295                 300                 


Pro Ala Pro Ser Pro Ala Ser Pro Ala Ala Ala Ser Pro Ser Ala Pro 
305                 310                 315                 320 


Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Met Gln Ile Phe 
                325                 330                 335     


Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
            340                 345                 350         


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
        355                 360                 365             


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
    370                 375                 380                 


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His 
385                 390                 395                 400 


Leu Val Leu Arg Leu Arg Ala Ala Ser Ala Pro Ala Ala Ser Pro Ser 
                405                 410                 415     


Ala Ala Ala Pro Ala Pro Ser Pro Ala Ser Pro Ala Pro Ala Ser Pro 
            420                 425                 430         


Ala Ser Ala Pro Ser Ala Pro Ala Ser Ala Pro Pro Ala Ala Ser Pro 
        435                 440                 445             


Ser Ala Pro Ala Ala Ser Pro Ser Pro Ala Ala Pro Ala Pro Ser Pro 
    450                 455                 460                 


Ala Ser Cys Ala Ala Ala Ser Pro Ser Ala Pro Ala Ala Ser Pro Ser 
465                 470                 475                 480 


Pro Ala Ala Pro Ala Pro Ser 
                485         


<210>  48
<211>  275
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  183422  N-terminal His-Tag/Sumo/77404 with S57C

<400>  48

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Met Arg Ile Trp Val His Thr Leu Thr 
        115                 120                 125             


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
    130                 135                 140                 


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
145                 150                 155                 160 


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
                165                 170                 175     


Asp Tyr Asn Ile Asn Pro Lys Leu Ser Leu His Leu Val Leu Arg Leu 
            180                 185                 190         


Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val His Thr Gln Thr Gly 
        195                 200                 205             


Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val 
    210                 215                 220                 


Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg 
225                 230                 235                 240 


Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp 
                245                 250                 255     


Tyr Asn Ile Gly Trp Gln Ala Pro Leu His Leu Val Leu Arg Leu Arg 
            260                 265                 270         


Ala Ala Cys 
        275 


<210>  49
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  183423  N-terminal His-Tag/Sumo/77404 with S57C

<400>  49

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Met Arg Ile Trp Val His Thr Leu Thr 
        115                 120                 125             


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
    130                 135                 140                 


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln 
145                 150                 155                 160 


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
                165                 170                 175     


Asp Tyr Asn Ile Asn Pro Lys Leu Ser Leu His Leu Val Leu Arg Leu 
            180                 185                 190         


Arg Ala Ala Gly Ile Gly Met Gln Ile Phe Val His Thr Gln Thr Gly 
        195                 200                 205             


Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val 
    210                 215                 220                 


Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg 
225                 230                 235                 240 


Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Cys Asp 
                245                 250                 255     


Tyr Asn Ile Gly Trp Gln Ala Pro Leu His Leu Val Leu Arg Leu Arg 
            260                 265                 270         


Ala Ala 
        


<210>  50
<211>  273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  184168  N-terminal His-Tag/Sumo/138800 with c-terminal Cys

<400>  50

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Met Gln Ile Phe Val His Thr Asp Thr 
        115                 120                 125             


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
    130                 135                 140                 


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Gln Asp Gln Gln 
145                 150                 155                 160 


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
                165                 170                 175     


Asp Tyr Asn Ile Asp Pro Gln Leu Lys Leu His Leu Val Leu Arg Leu 
            180                 185                 190         


Arg Ala Ala Gly Gly Met Gln Ile Phe Val Thr Thr Gln Thr Gly Lys 
        195                 200                 205             


Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys 
    210                 215                 220                 


Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu 
225                 230                 235                 240 


Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr 
                245                 250                 255     


Asn Ile Asp Tyr Arg Phe Leu His Leu Val Leu Arg Leu Arg Ala Ala 
            260                 265                 270         


Cys 
    


<210>  51
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  184169 - N-terminal His-Tag/Sumo/138800 with S57C

<400>  51

Met Gly Ser Ser His His His His His His Gly Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser Ala Ser Met Ser Asp Ser Glu Val Asn Gln Glu Ala Lys 
            20                  25                  30          


Pro Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys 
        35                  40                  45              


Val Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr 
    50                  55                  60                  


Pro Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu 
65                  70                  75                  80  


Met Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp 
                85                  90                  95      


Gln Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala 
            100                 105                 110         


His Arg Glu Gln Ile Gly Gly Met Gln Ile Phe Val His Thr Asp Thr 
        115                 120                 125             


Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn 
    130                 135                 140                 


Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Gln Asp Gln Gln 
145                 150                 155                 160 


Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser 
                165                 170                 175     


Asp Tyr Asn Ile Asp Pro Gln Leu Lys Leu His Leu Val Leu Arg Leu 
            180                 185                 190         


Arg Ala Ala Gly Gly Met Gln Ile Phe Val Thr Thr Gln Thr Gly Lys 
        195                 200                 205             


Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys 
    210                 215                 220                 


Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu 
225                 230                 235                 240 


Ile Trp Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Cys Asp Tyr 
                245                 250                 255     


Asn Ile Asp Tyr Arg Phe Leu His Leu Val Leu Arg Leu Arg Ala Ala 
            260                 265                 270         


<210>  52
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  StrepTag extension for purification

<400>  52

Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10  


<210>  53
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Affilin 190761

<400>  53

Met Gln Ile Phe Val His Thr Arg Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Ala His Gln 
    50                  55                  60                  


Pro Gln Leu His Leu Val Leu Arg Leu Arg Ala Ala Met Gln Ile Phe 
65                  70                  75                  80  


Val Gln Thr Met Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser 
                85                  90                  95      


Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile 
            100                 105                 110         


Pro Pro Asp Gln Gln Arg Leu Ile Trp Ala Gly Lys Gln Leu Glu Asp 
        115                 120                 125             


Gly Arg Thr Leu Ser Asp Tyr Asn Ile Asp Tyr Arg Tyr Leu His Leu 
    130                 135                 140                 


Val Leu Arg Leu Arg Ala Ala 
145                 150     


