                         SEQUENCE LISTING

<110>  The Regents of the University of California
 
<120>  VAULT PARTICLES HAVING A MODIFIED R8 FLEXIBLE REGION

<130>  034044.176P1

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R8 flexible region of human major vault protein (MVP)

<400>  1
aacaaggggc aggaccctct ggcagacagg ggtgagaagg acacagctaa gagcctccag       60

cccttg                                                                  66


<210>  2
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified R8 flexible region having an AfeI restriction enzyme 
       site inserted therein

<400>  2
aacaaggggc aggaccctct ggcagacagg ggtagcgctg agaaggacac agctaagagc       60

ctccagccct tg                                                           72


<210>  3
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human MVP from amino acids 428-449

<400>  3

Asn Lys Gly Gln Asp Pro Leu Ala Asp Arg Gly Glu Lys Asp Thr Ala 
1               5                   10                  15      


Lys Ser Leu Gln Pro Leu 
            20          


<210>  4
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Example of R8 flexible loop sequence

<400>  4

Val Glu Glu Leu Leu Asn Lys Gly Gln Asp Pro Leu Ala Asp Arg Gly 
1               5                   10                  15      


Glu Lys Asp Thr Ala Lys Ser Leu Gln Pro Leu Ala Pro Arg Asn Lys 
            20                  25                  30          


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Example of flexible amino acid linker

<400>  5

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Example of flexible amino acid linker

<400>  6

Gly Phe Leu Gly Leu 
1               5   


<210>  7
<211>  893
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human Major Vault Protein (MVP)

<400>  7

Met Ala Thr Glu Glu Phe Ile Ile Arg Ile Pro Pro Tyr His Tyr Ile 
1               5                   10                  15      


His Val Leu Asp Gln Asn Ser Asn Val Ser Arg Val Glu Val Gly Pro 
            20                  25                  30          


Lys Thr Tyr Ile Arg Gln Asp Asn Glu Arg Val Leu Phe Ala Pro Met 
        35                  40                  45              


Arg Met Val Thr Val Pro Pro Arg His Tyr Cys Thr Val Ala Asn Pro 
    50                  55                  60                  


Val Ser Arg Asp Ala Gln Gly Leu Val Leu Phe Asp Val Thr Gly Gln 
65                  70                  75                  80  


Val Arg Leu Arg His Ala Asp Leu Glu Ile Arg Leu Ala Gln Asp Pro 
                85                  90                  95      


Phe Pro Leu Tyr Pro Gly Glu Val Leu Glu Lys Asp Ile Thr Pro Leu 
            100                 105                 110         


Gln Val Val Leu Pro Asn Thr Ala Leu His Leu Lys Ala Leu Leu Asp 
        115                 120                 125             


Phe Glu Asp Lys Asp Gly Asp Lys Val Val Ala Gly Asp Glu Trp Leu 
    130                 135                 140                 


Phe Glu Gly Pro Gly Thr Tyr Ile Pro Arg Lys Glu Val Glu Val Val 
145                 150                 155                 160 


Glu Ile Ile Gln Ala Thr Ile Ile Arg Gln Asn Gln Ala Leu Arg Leu 
                165                 170                 175     


Arg Ala Arg Lys Glu Cys Trp Asp Arg Asp Gly Lys Glu Arg Val Thr 
            180                 185                 190         


Gly Glu Glu Trp Leu Val Thr Thr Val Gly Ala Tyr Leu Pro Ala Val 
        195                 200                 205             


Phe Glu Glu Val Leu Asp Leu Val Asp Ala Val Ile Leu Thr Glu Lys 
    210                 215                 220                 


Thr Ala Leu His Leu Arg Ala Arg Arg Asn Phe Arg Asp Phe Arg Gly 
225                 230                 235                 240 


Val Ser Arg Arg Thr Gly Glu Glu Trp Leu Val Thr Val Gln Asp Thr 
                245                 250                 255     


Glu Ala His Val Pro Asp Val His Glu Glu Val Leu Gly Val Val Pro 
            260                 265                 270         


Ile Thr Thr Leu Gly Pro His Asn Tyr Cys Val Ile Leu Asp Pro Val 
        275                 280                 285             


Gly Pro Asp Gly Lys Asn Gln Leu Gly Gln Lys Arg Val Val Lys Gly 
    290                 295                 300                 


Glu Lys Ser Phe Phe Leu Gln Pro Gly Glu Gln Leu Glu Gln Gly Ile 
305                 310                 315                 320 


Gln Asp Val Tyr Val Leu Ser Glu Gln Gln Gly Leu Leu Leu Arg Ala 
                325                 330                 335     


Leu Gln Pro Leu Glu Glu Gly Glu Asp Glu Glu Lys Val Ser His Gln 
            340                 345                 350         


Ala Gly Asp His Trp Leu Ile Arg Gly Pro Leu Glu Tyr Val Pro Ser 
        355                 360                 365             


Ala Lys Val Glu Val Val Glu Glu Arg Gln Ala Ile Pro Leu Asp Glu 
    370                 375                 380                 


Asn Glu Gly Ile Tyr Val Gln Asp Val Lys Thr Gly Lys Val Arg Ala 
385                 390                 395                 400 


Val Ile Gly Ser Thr Tyr Met Leu Thr Gln Asp Glu Val Leu Trp Glu 
                405                 410                 415     


Lys Glu Leu Pro Pro Gly Val Glu Glu Leu Leu Asn Lys Gly Gln Asp 
            420                 425                 430         


Pro Leu Ala Asp Arg Gly Glu Lys Asp Thr Ala Lys Ser Leu Gln Pro 
        435                 440                 445             


Leu Ala Pro Arg Asn Lys Thr Arg Val Val Ser Tyr Arg Val Pro His 
    450                 455                 460                 


Asn Ala Ala Val Gln Val Tyr Asp Tyr Arg Glu Lys Arg Ala Arg Val 
465                 470                 475                 480 


Val Phe Gly Pro Glu Leu Val Ser Leu Gly Pro Glu Glu Gln Phe Thr 
                485                 490                 495     


Val Leu Ser Leu Ser Ala Gly Arg Pro Lys Arg Pro His Ala Arg Arg 
            500                 505                 510         


Ala Leu Cys Leu Leu Leu Gly Pro Asp Phe Phe Thr Asp Val Ile Thr 
        515                 520                 525             


Ile Glu Thr Ala Asp His Ala Arg Leu Gln Leu Gln Leu Ala Tyr Asn 
    530                 535                 540                 


Trp His Phe Glu Val Asn Asp Arg Lys Asp Pro Gln Glu Thr Ala Lys 
545                 550                 555                 560 


Leu Phe Ser Val Pro Asp Phe Val Gly Asp Ala Cys Lys Ala Ile Ala 
                565                 570                 575     


Ser Arg Val Arg Gly Ala Val Ala Ser Val Thr Phe Asp Asp Phe His 
            580                 585                 590         


Lys Asn Ser Ala Arg Ile Ile Arg Thr Ala Val Phe Gly Phe Glu Thr 
        595                 600                 605             


Ser Glu Ala Lys Gly Pro Asp Gly Met Ala Leu Pro Arg Pro Arg Asp 
    610                 615                 620                 


Gln Ala Val Phe Pro Gln Asn Gly Leu Val Val Ser Ser Val Asp Val 
625                 630                 635                 640 


Gln Ser Val Glu Pro Val Asp Gln Arg Thr Arg Asp Ala Leu Gln Arg 
                645                 650                 655     


Ser Val Gln Leu Ala Ile Glu Ile Thr Thr Asn Ser Gln Glu Ala Ala 
            660                 665                 670         


Ala Lys His Glu Ala Gln Arg Leu Glu Gln Glu Ala Arg Gly Arg Leu 
        675                 680                 685             


Glu Arg Gln Lys Ile Leu Asp Gln Ser Glu Ala Glu Lys Ala Arg Lys 
    690                 695                 700                 


Glu Leu Leu Glu Leu Glu Ala Leu Ser Met Ala Val Glu Ser Thr Gly 
705                 710                 715                 720 


Thr Ala Lys Ala Glu Ala Glu Ser Arg Ala Glu Ala Ala Arg Ile Glu 
                725                 730                 735     


Gly Glu Gly Ser Val Leu Gln Ala Lys Leu Lys Ala Gln Ala Leu Ala 
            740                 745                 750         


Ile Glu Thr Glu Ala Glu Leu Gln Arg Val Gln Lys Val Arg Glu Leu 
        755                 760                 765             


Glu Leu Val Tyr Ala Arg Ala Gln Leu Glu Leu Glu Val Ser Lys Ala 
    770                 775                 780                 


Gln Gln Leu Ala Glu Val Glu Val Lys Lys Phe Lys Gln Met Thr Glu 
785                 790                 795                 800 


Ala Ile Gly Pro Ser Thr Ile Arg Asp Leu Ala Val Ala Gly Pro Glu 
                805                 810                 815     


Met Gln Val Lys Leu Leu Gln Ser Leu Gly Leu Lys Ser Thr Leu Ile 
            820                 825                 830         


Thr Asp Gly Ser Thr Pro Ile Asn Leu Phe Asn Thr Ala Phe Gly Leu 
        835                 840                 845             


Leu Gly Met Gly Pro Glu Gly Gln Pro Leu Gly Arg Arg Val Ala Ser 
    850                 855                 860                 


Gly Pro Ser Pro Gly Glu Gly Ile Ser Pro Gln Ser Ala Gln Ala Pro 
865                 870                 875                 880 


Gln Ala Pro Gly Asp Asn His Val Val Pro Val Leu Arg 
                885                 890             


<210>  8
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human Major Vault Protein (MVP) forward primer

<400>  8
gctgagaagg acacagctaa gagcct                                            26


<210>  9
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human Major Vault Protein (MVP) reverse primer

<400>  9
gctacccctg tctgccagag ggtcct                                            26


<210>  10
<211>  204
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Gag1-M1 insert


<220>
<221>  CDS
<222>  (1)..(204)

<400>  10
atg acc ccc cgg acc ctg aac gcc tgg gtc aag gtg gtg gaa gag aag         48
Met Thr Pro Arg Thr Leu Asn Ala Trp Val Lys Val Val Glu Glu Lys           
1               5                   10                  15                

gcc ttc agc ccc gaa gtg atc ccc atg ttc acc gcc ctg agc gag ggc         96
Ala Phe Ser Pro Glu Val Ile Pro Met Phe Thr Ala Leu Ser Glu Gly           
            20                  25                  30                    

gcc acc ccc agc gac ctg aac acc atg ctg aat acc atc ggc ggc cac        144
Ala Thr Pro Ser Asp Leu Asn Thr Met Leu Asn Thr Ile Gly Gly His           
        35                  40                  45                        

cag gcc gcc atg cag atg ctg aag gac acc atc aac gaa gag gcc gcc        192
Gln Ala Ala Met Gln Met Leu Lys Asp Thr Ile Asn Glu Glu Ala Ala           
    50                  55                  60                            

gag tgg gac cgg                                                        204
Glu Trp Asp Arg                                                           
65                                                                        


<210>  11
<211>  68
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11

Met Thr Pro Arg Thr Leu Asn Ala Trp Val Lys Val Val Glu Glu Lys 
1               5                   10                  15      


Ala Phe Ser Pro Glu Val Ile Pro Met Phe Thr Ala Leu Ser Glu Gly 
            20                  25                  30          


Ala Thr Pro Ser Asp Leu Asn Thr Met Leu Asn Thr Ile Gly Gly His 
        35                  40                  45              


Gln Ala Ala Met Gln Met Leu Lys Asp Thr Ile Asn Glu Glu Ala Ala 
    50                  55                  60                  


Glu Trp Asp Arg 
65              


<210>  12
<211>  261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Gag2-M1 insert


<220>
<221>  CDS
<222>  (1)..(261)

<400>  12
atg aac ccc ccc atc ccc gtg ggc gac atc tac aag cgg tgg atc atc         48
Met Asn Pro Pro Ile Pro Val Gly Asp Ile Tyr Lys Arg Trp Ile Ile           
1               5                   10                  15                

ctg ggc ctg gac aag atc gtg cgg atg tac agc ccc acc agc atc ctg         96
Leu Gly Leu Asp Lys Ile Val Arg Met Tyr Ser Pro Thr Ser Ile Leu           
            20                  25                  30                    

gac atc cgg cag ggc ccc aaa gag ccc ttc cgg gac tac gtg gac cgg        144
Asp Ile Arg Gln Gly Pro Lys Glu Pro Phe Arg Asp Tyr Val Asp Arg           
        35                  40                  45                        

ttc ttc aag gtg ctg cgg gcc gag cag gcc acc cag gac gtg aag aac        192
Phe Phe Lys Val Leu Arg Ala Glu Gln Ala Thr Gln Asp Val Lys Asn           
    50                  55                  60                            

tgg atg acc gac acc ctg ctg gtg cag aac gcc aac ccc gac tgc aag        240
Trp Met Thr Asp Thr Leu Leu Val Gln Asn Ala Asn Pro Asp Cys Lys           
65                  70                  75                  80            

acc atc ctg aga gcc ctg ggc                                            261
Thr Ile Leu Arg Ala Leu Gly                                               
                85                                                        


<210>  13
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Met Asn Pro Pro Ile Pro Val Gly Asp Ile Tyr Lys Arg Trp Ile Ile 
1               5                   10                  15      


Leu Gly Leu Asp Lys Ile Val Arg Met Tyr Ser Pro Thr Ser Ile Leu 
            20                  25                  30          


Asp Ile Arg Gln Gly Pro Lys Glu Pro Phe Arg Asp Tyr Val Asp Arg 
        35                  40                  45              


Phe Phe Lys Val Leu Arg Ala Glu Gln Ala Thr Gln Asp Val Lys Asn 
    50                  55                  60                  


Trp Met Thr Asp Thr Leu Leu Val Gln Asn Ala Asn Pro Asp Cys Lys 
65                  70                  75                  80  


Thr Ile Leu Arg Ala Leu Gly 
                85          


<210>  14
<211>  261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Env-M1 insert


<220>
<221>  CDS
<222>  (1)..(261)

<400>  14
atg ggc ttt ctg ggc gtg gcc ggc agc aca atg gga gcc gcc agc atc         48
Met Gly Phe Leu Gly Val Ala Gly Ser Thr Met Gly Ala Ala Ser Ile           
1               5                   10                  15                

acc ctg acc gtg cag gcc aga cag ctg ctg agc ggc atc gtg cag cag         96
Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln           
            20                  25                  30                    

cag agc aac ctg ctg aga gct atc gag gcc cag cag cat ctg ctg aag        144
Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Lys           
        35                  40                  45                        

ctg acc gtg tgg ggc atc aag cag ctg cag acc cgg gtg ctg gcc atc        192
Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile           
    50                  55                  60                            

gag aga tac ctg aag gac cag cag ctc ctg ggc ctg tgg ggc tgc agc        240
Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Leu Trp Gly Cys Ser           
65                  70                  75                  80            

ggc aag ctg atc tgc ccc acc                                            261
Gly Lys Leu Ile Cys Pro Thr                                               
                85                                                        


<210>  15
<211>  87
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Met Gly Phe Leu Gly Val Ala Gly Ser Thr Met Gly Ala Ala Ser Ile 
1               5                   10                  15      


Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
            20                  25                  30          


Gln Ser Asn Leu Leu Arg Ala Ile Glu Ala Gln Gln His Leu Leu Lys 
        35                  40                  45              


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Thr Arg Val Leu Ala Ile 
    50                  55                  60                  


Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Leu Trp Gly Cys Ser 
65                  70                  75                  80  


Gly Lys Leu Ile Cys Pro Thr 
                85          


<210>  16
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Nef-M1 insert


<220>
<221>  CDS
<222>  (1)..(132)

<400>  16
atg cgg cag gaa atc ctg gac ctc tgg gtg tac cac acc cag gga ttc         48
Met Arg Gln Glu Ile Leu Asp Leu Trp Val Tyr His Thr Gln Gly Phe           
1               5                   10                  15                

ttc cca gac tgg cag aac tac acc ccc gga ccc ggc atc aga tac ccc         96
Phe Pro Asp Trp Gln Asn Tyr Thr Pro Gly Pro Gly Ile Arg Tyr Pro           
            20                  25                  30                    

ctg acc ttc ggc tgg tgc tac aag ctg gtg ccc gtg                        132
Leu Thr Phe Gly Trp Cys Tyr Lys Leu Val Pro Val                           
        35                  40                                            


<210>  17
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17

Met Arg Gln Glu Ile Leu Asp Leu Trp Val Tyr His Thr Gln Gly Phe 
1               5                   10                  15      


Phe Pro Asp Trp Gln Asn Tyr Thr Pro Gly Pro Gly Ile Arg Tyr Pro 
            20                  25                  30          


Leu Thr Phe Gly Trp Cys Tyr Lys Leu Val Pro Val 
        35                  40                  


<210>  18
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Gag1-M1 forward primer

<400>  18
ggcagacagg ggtagcatga ccccccggac cctgaacg                               38


<210>  19
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Gag1-M1 reverse primer

<400>  19
gctgtgtcct tctcagcccg gtcccactcg gcggcct                                37


<210>  20
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Gag2-M1 forward primer

<400>  20
ggcagacagg ggtagcatga acccccccat ccccgt                                 36


<210>  21
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Gag2-M1 reverse primer

<400>  21
gctgtgtcct tctcagcgcc cagggctctc aggatgg                                37


<210>  22
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Env-M1 forward primer

<400>  22
ggcagacagg ggtagcatgg gctttctggg cgtggc                                 36


<210>  23
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Env-M1 reverse primer

<400>  23
gctgtgtcct tctcagcggt ggggcagatc agcttg                                 36


<210>  24
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Nef-M1 forward primer

<400>  24
ggcagacagg ggtagcatgc ggcaggaaat cctgga                                 36


<210>  25
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV Nef-M1 reverse primer

<400>  25
gctgtgtcct tctcagccac gggcaccagc ttgtag                                 36


<210>  26
<211>  708
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry insert


<220>
<221>  CDS
<222>  (1)..(708)

<400>  26
atg gtg agc aag ggc gag gag gat aac atg gcc atc atc aag gag ttc         48
Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe           
1               5                   10                  15                

atg cgc ttc aag gtg cac atg gag ggc tcc gtg aac ggc cac gag ttc         96
Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe           
            20                  25                  30                    

gag atc gag ggc gag ggc gag ggc cgc ccc tac gag ggc acc cag acc        144
Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr           
        35                  40                  45                        

gcc aag ctg aag gtg acc aag ggt ggc ccc ctg ccc ttc gcc tgg gac        192
Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp           
    50                  55                  60                            

atc ctg tcc cct cag ttc atg tac ggc tcc aag gcc tac gtg aag cac        240
Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His           
65                  70                  75                  80            

ccc gcc gac atc ccc gac tac ttg aag ctg tcc ttc ccc gag ggc ttc        288
Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe           
                85                  90                  95                

aag tgg gag cgc gtg atg aac ttc gag gac ggc ggc gtg gtg acc gtg        336
Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val           
            100                 105                 110                   

acc cag gac tcc tcc ctg cag gac ggc gag ttc atc tac aag gtg aag        384
Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys           
        115                 120                 125                       

ctg cgc ggc acc aac ttc ccc tcc gac ggc ccc gta atg cag aag aag        432
Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys           
    130                 135                 140                           

acc atg ggc tgg gag gcc tcc tcc gag cgg atg tac ccc gag gac ggc        480
Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly           
145                 150                 155                 160           

gcc ctg aag ggc gag atc aag cag agg ctg aag ctg aag gac ggc ggc        528
Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly           
                165                 170                 175               

cac tac gac gct gag gtc aag acc acc tac aag gcc aag aag ccc gtg        576
His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val           
            180                 185                 190                   

cag ctg ccc ggc gcc tac aac gtc aac atc aag ttg gac atc acc tcc        624
Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser           
        195                 200                 205                       

cac aac gag gac tac acc atc gtg gaa cag tac gaa cgc gcc gag ggc        672
His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly           
    210                 215                 220                           

cgc cac tcc acc ggc ggc atg gac gag ctg tac aag                        708
Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys                           
225                 230                 235                               


<210>  27
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  27

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     


<210>  28
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry forward primer

<400>  28
ggcagacagg ggtagcatgg tgagcaaggg cgaggaggat                             40


<210>  29
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mCherry reverse primer

<400>  29
gctgtgtcct tctcagcctt gtacagctcg tccatg                                 36


<210>  30
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AfeI site forward primer

<400>  30
gctttggcgc cccggaacaa gacccgt                                           27


<210>  31
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AfeI site reverse primer

<400>  31
gctgttcagc agctcctcca ccccggga                                          28


