               SEQUENCE LISTING

<110> Thena Biotech S.r.l.

<120> NEW FUSION-PROTEINS BASED ON HUMAN FERRITIN AND PROTEASE-CLEAVABLE PEPTIDES AND THEIR USE AS CHEMOTHERAPEUTICS CARRIERS

<130> BI5162R

<160> 18

<170> BiSSAP 1.3.6

<210> 1
<211> 183
<212> PRT
<213> Homo sapiens


<220> 
<223> >1 aminoacid sequence of the native HFt

<400> 1
Met Thr Thr Ala Ser Thr Ser Gln Val Arg Gln Asn Tyr His Gln Asp 
1               5                   10                  15      
Ser Glu Ala Ala Ile Asn Arg Gln Ile Asn Leu Glu Leu Tyr Ala Ser 
            20                  25                  30          
Tyr Val Tyr Leu Ser Met Ser Tyr Tyr Phe Asp Arg Asp Asp Val Ala 
        35                  40                  45              
Leu Lys Asn Phe Ala Lys Tyr Phe Leu His Gln Ser His Glu Glu Arg 
    50                  55                  60                  
Glu His Ala Glu Lys Leu Met Lys Leu Gln Asn Gln Arg Gly Gly Arg 
65                  70                  75                  80  
Ile Phe Leu Gln Asp Ile Lys Lys Pro Asp Cys Asp Asp Trp Glu Ser 
                85                  90                  95      
Gly Leu Asn Ala Met Glu Cys Ala Leu His Leu Glu Lys Asn Val Asn 
            100                 105                 110         
Gln Ser Leu Leu Glu Leu His Lys Leu Ala Thr Asp Lys Asn Asp Pro 
        115                 120                 125             
His Leu Cys Asp Phe Ile Glu Thr His Tyr Leu Asn Glu Gln Val Lys 
    130                 135                 140                 
Ala Ile Lys Glu Leu Gly Asp His Val Thr Asn Leu Arg Lys Met Gly 
145                 150                 155                 160 
Ala Pro Glu Ser Gly Leu Ala Glu Tyr Leu Phe Asp Lys His Thr Leu 
                165                 170                 175     
Gly Asp Ser Asp Asn Glu Ser 
            180             

<210> 2
<211> 183
<212> PRT
<213> Artificial Sequence


<220> 
<223> >2 HFT variant

<400> 2
Met Thr Thr Ala Ser Thr Ser Gln Val Arg Gln Asn Tyr His Gln Asp 
1               5                   10                  15      
Ser Glu Ala Ala Ile Asn Arg Gln Ile Asn Leu Glu Leu Tyr Ala Ser 
            20                  25                  30          
Tyr Val Tyr Leu Ser Met Ser Tyr Tyr Phe Asp Arg Asp Asp Val Ala 
        35                  40                  45              
Leu Lys Asn Phe Ala Lys Tyr Phe Leu His Gln Ser His Glu Glu Arg 
    50                  55                  60                  
Glu His Ala Glu Lys Leu Met Cys Leu Gln Asn Gln Arg Gly Gly Arg 
65                  70                  75                  80  
Ile Phe Leu Gln Asp Ile Lys Lys Pro Asp Ser Asp Asp Trp Glu Ser 
                85                  90                  95      
Gly Leu Asn Ala Met Glu Ser Ala Leu His Leu Glu Lys Asn Val Asn 
            100                 105                 110         
Gln Ser Leu Leu Glu Leu His Lys Leu Ala Thr Asp Lys Asn Asp Pro 
        115                 120                 125             
His Leu Ser Asp Phe Ile Glu Thr His Tyr Leu Asn Glu Gln Val Cys 
    130                 135                 140                 
Ala Ile Lys Glu Leu Gly Asp His Val Thr Asn Leu Arg Lys Met Gly 
145                 150                 155                 160 
Ala Pro Glu Ser Gly Leu Ala Glu Tyr Leu Phe Asp Lys His Thr Leu 
                165                 170                 175     
Gly Asp Ser Asp Asn Glu Cys 
            180             

<210> 3
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> >SEQ 3

<400> 3
Gly Pro Leu Gly Ile Ala Gly Gln 
1               5               

<210> 4
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 4

<400> 4
Gly Pro Gln Gly Ile Trp Gly Gln 
1               5               

<210> 5
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> >SEq 5

<400> 5
Pro Leu Gly Leu Ala Gly 
1               5       

<210> 6
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 6

<400> 6
Pro Val Gly Leu Ile Gly 
1               5       

<210> 7
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 7

<400> 7
Cys Gly Leu Asp Asp 
1               5   

<210> 8
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 8

<400> 8
Gly Pro Leu Gly Ile Ala Gly Gln Gly Pro Leu Gly Ile Ala Gly Gln 
1               5                   10                  15      



<210> 9
<211> 42
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 9

<400> 9
Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      
Ser Ala Pro Ala Glu Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro 
            20                  25                  30          
Ala Ala Pro Ala Pro Ser Ala Pro Ala Glu 
        35                  40          

<210> 10
<211> 40
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 10

<400> 10
Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Glu Pro Ala Pro 
1               5                   10                  15      
Ser Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala 
            20                  25                  30          
Glu Pro Ala Pro Ser Ala Pro Ala 
        35                  40  

<210> 11
<211> 73
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 11

<400> 11
Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      
Ser Ala Pro Ala Glu Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro 
            20                  25                  30          
Ala Ala Pro Ala Pro Ser Ala Pro Ala Glu Ala Ser Pro Ala Ala Pro 
        35                  40                  45              
Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro Ala Glu Ala 
    50                  55                  60                  
Ser Pro Ala Ala Pro Ala Pro Ala Ser 
65                  70              

<210> 12
<211> 42
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 12

<400> 12
Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      
Ser Ala Pro Ala Asp Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro 
            20                  25                  30          
Ala Ala Pro Ala Pro Ser Ala Pro Ala Asp 
        35                  40          

<210> 13
<211> 40
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 13

<400> 13
Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Asp Pro Ala Pro 
1               5                   10                  15      
Ser Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala 
            20                  25                  30          
Asp Pro Ala Pro Ser Ala Pro Ala 
        35                  40  

<210> 14
<211> 73
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 14

<400> 14
Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      
Ser Ala Pro Ala Asp Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro 
            20                  25                  30          
Ala Ala Pro Ala Pro Ser Ala Pro Ala Asp Ala Ser Pro Ala Ala Pro 
        35                  40                  45              
Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro Ala Asp Ala 
    50                  55                  60                  
Ser Pro Ala Ala Pro Ala Pro Ala Ser 
65                  70              

<210> 15
<211> 183
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 15

<400> 15
Met Thr Thr Ala Ser Thr Ser Gln Val Arg Gln Asn Tyr His Gln Asp 
1               5                   10                  15      
Ser Glu Ala Ala Ile Asn Arg Gln Ile Asn Leu Glu Leu Tyr Ala Ser 
            20                  25                  30          
Tyr Val Tyr Leu Ser Met Ser Tyr Tyr Phe Asp Arg Asp Asp Val Ala 
        35                  40                  45              
Leu Lys Asn Phe Ala Cys Tyr Phe Leu His Gln Ser His Glu Glu Arg 
    50                  55                  60                  
Glu His Ala Glu Lys Leu Met Cys Leu Gln Asn Gln Arg Gly Gly Arg 
65                  70                  75                  80  
Ile Phe Leu Gln Asp Ile Lys Lys Pro Asp Ser Asp Asp Trp Glu Ser 
                85                  90                  95      
Gly Leu Asn Ala Met Glu Ser Ala Leu His Leu Glu Lys Asn Val Asn 
            100                 105                 110         
Gln Ser Leu Leu Glu Leu His Lys Leu Ala Thr Asp Lys Asn Asp Pro 
        115                 120                 125             
His Leu Ser Asp Phe Ile Glu Cys His Tyr Leu Asn Glu Gln Val Cys 
    130                 135                 140                 
Ala Ile Lys Glu Leu Gly Asp His Val Thr Asn Leu Arg Lys Met Gly 
145                 150                 155                 160 
Ala Pro Glu Ser Gly Leu Ala Glu Tyr Leu Phe Asp Lys His Thr Leu 
                165                 170                 175     
Gly Asp Ser Asp Asn Glu Ser 
            180             

<210> 16
<211> 229
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 16

<400> 16
Met Thr Thr Ala Ser Thr Ser Gln Val Arg Gln Asn Tyr His Gln Asp 
1               5                   10                  15      
Ser Glu Ala Ala Ile Asn Arg Gln Ile Asn Leu Glu Leu Tyr Ala Ser 
            20                  25                  30          
Tyr Val Tyr Leu Ser Met Ser Tyr Tyr Phe Asp Arg Asp Asp Val Ala 
        35                  40                  45              
Leu Lys Asn Phe Ala Lys Tyr Phe Leu His Gln Ser His Glu Glu Arg 
    50                  55                  60                  
Glu His Ala Glu Lys Leu Met Lys Leu Gln Asn Gln Arg Gly Gly Arg 
65                  70                  75                  80  
Ile Phe Leu Gln Asp Ile Lys Lys Pro Asp Cys Asp Asp Trp Glu Ser 
                85                  90                  95      
Gly Leu Asn Ala Met Glu Cys Ala Leu His Leu Glu Lys Asn Val Asn 
            100                 105                 110         
Gln Ser Leu Leu Glu Leu His Lys Leu Ala Thr Asp Lys Asn Asp Pro 
        115                 120                 125             
His Leu Cys Asp Phe Ile Glu Thr His Tyr Leu Asn Glu Gln Val Lys 
    130                 135                 140                 
Ala Ile Lys Glu Leu Gly Asp His Val Thr Asn Leu Arg Lys Met Gly 
145                 150                 155                 160 
Ala Pro Glu Ser Gly Leu Ala Glu Tyr Leu Phe Asp Lys His Thr Leu 
                165                 170                 175     
Gly Asp Ser Asp Asn Glu Ser Pro Leu Gly Leu Ala Gly Ala Ser Pro 
            180                 185                 190         
Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro 
        195                 200                 205             
Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala 
    210                 215                 220                 
Pro Ser Ala Pro Ala 
225                 

<210> 17
<211> 229
<212> PRT
<213> Artificial Sequence


<220> 
<223> >seq 17

<400> 17
Met Thr Thr Ala Ser Thr Ser Gln Val Arg Gln Asn Tyr His Gln Asp 
1               5                   10                  15      
Ser Glu Ala Ala Ile Asn Arg Gln Ile Asn Leu Glu Leu Tyr Ala Ser 
            20                  25                  30          
Tyr Val Tyr Leu Ser Met Ser Tyr Tyr Phe Asp Arg Asp Asp Val Ala 
        35                  40                  45              
Leu Lys Asn Phe Ala Lys Tyr Phe Leu His Gln Ser His Glu Glu Arg 
    50                  55                  60                  
Glu His Ala Glu Lys Leu Met Lys Leu Gln Asn Gln Arg Gly Gly Arg 
65                  70                  75                  80  
Ile Phe Leu Gln Asp Ile Lys Lys Pro Asp Cys Asp Asp Trp Glu Ser 
                85                  90                  95      
Gly Leu Asn Ala Met Glu Cys Ala Leu His Leu Glu Lys Asn Val Asn 
            100                 105                 110         
Gln Ser Leu Leu Glu Leu His Lys Leu Ala Thr Asp Lys Asn Asp Pro 
        115                 120                 125             
His Leu Cys Asp Phe Ile Glu Thr His Tyr Leu Asn Glu Gln Val Lys 
    130                 135                 140                 
Ala Ile Lys Glu Leu Gly Asp His Val Thr Asn Leu Arg Lys Met Gly 
145                 150                 155                 160 
Ala Pro Glu Ser Gly Leu Ala Glu Tyr Leu Phe Asp Lys His Thr Leu 
                165                 170                 175     
Gly Asp Ser Asp Asn Glu Ser Pro Leu Gly Leu Ala Gly Ala Ser Pro 
            180                 185                 190         
Ala Ala Pro Ala Pro Ala Ser Pro Ala Glu Pro Ala Pro Ser Ala Pro 
        195                 200                 205             
Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Glu Pro Ala 
    210                 215                 220                 
Pro Ser Ala Pro Ala 
225                 

<210> 18
<211> 183
<212> PRT
<213> Artificial Sequence


<220> 
<223> HFt Glu SEQ ID NO 18

<400> 18
Met Thr Thr Ala Ser Thr Ser Gln Val Arg Gln Asn Tyr His Gln Asp 
1               5                   10                  15      
Ser Glu Ala Ala Ile Asn Arg Gln Ile Asn Leu Glu Leu Tyr Ala Ser 
            20                  25                  30          
Tyr Val Tyr Leu Ser Met Ser Tyr Tyr Phe Asp Arg Asp Asp Val Ala 
        35                  40                  45              
Leu Lys Asn Phe Ala Glu Tyr Phe Leu His Gln Ser His Glu Glu Arg 
    50                  55                  60                  
Glu His Ala Glu Lys Leu Met Glu Leu Gln Asn Gln Arg Gly Gly Arg 
65                  70                  75                  80  
Ile Phe Leu Gln Asp Ile Lys Lys Pro Asp Ser Asp Asp Trp Glu Ser 
                85                  90                  95      
Gly Leu Asn Ala Met Glu Ser Ala Leu His Leu Glu Lys Asn Val Asn 
            100                 105                 110         
Gln Ser Leu Leu Glu Leu His Lys Leu Ala Thr Asp Lys Asn Asp Pro 
        115                 120                 125             
His Leu Ser Asp Phe Ile Glu Glu His Tyr Leu Asn Glu Gln Val Glu 
    130                 135                 140                 
Ala Ile Lys Glu Leu Gly Asp His Val Thr Asn Leu Arg Lys Met Gly 
145                 150                 155                 160 
Ala Pro Glu Ser Gly Leu Ala Glu Tyr Leu Phe Asp Lys His Thr Leu 
                165                 170                 175     
Gly Asp Ser Asp Asn Glu Ser 
            180             

