                         SEQUENCE LISTING

<110>  ROQUETTE FRERES
 
<120>  A GENETICALLY MODIFIED BACILLUS SUBTILIS STRAIN, OPTIMIZED 
       VECTORS, AND USES THEREOF

<130>  BCT180311QT

<150>  EP 17306533.5
<151>  2017-11-06

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Optimized RBS

<400>  1
agaaaggagg attacat                                                      17


<210>  2
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Optimized translation initiation region

<400>  2
agaaaggagg attcgaa                                                      17


<210>  3
<211>  882
<212>  DNA
<213>  Clostridium cellulolyticum H10

<400>  3
atgaaacatg gtatatacta cgcatattgg gaacaagaat gggaagctga ttacaaatac       60

tatattgaga aggttgcaaa gcttggtttt gatattctag agattgcagc ttcaccgcta      120

cctttttaca gtgacattca gattaatgag ctcaaggcat gtgcccatgg caatggaatt      180

acacttacgg taggccatgg gcctagtgca gaacaaaacc tgtcttctcc cgaccccgat      240

attcgcaaaa atgctaaagc tttttatacc gatttactca aacgacttta caagctggat      300

gtacatttga taggtggggc tttatattct tattggccga tagattacac aaagacaatt      360

gataaaaaag gcgattggga acgcagcgtt gaaagtgttc gagaagttgc taaggtggcc      420

gaagcctgtg gagtggattt ctgcctagag gttcttaata gatttgagaa ttatttaatt      480

aacacagcac aagagggtgt agattttgta aaacaggttg accataacaa tgtaaaggta      540

atgcttgata ccttccatat gaatattgag gaagatagta tcggaggtgc aatcaggact      600

gcgggctctt acttgggaca tttacacact ggcgaatgta atcgtaaagt tcccggcaga      660

ggaagaattc catgggtaga aattggtgag gctcttgctg acataggtta taacggtagt      720

gttgttatgg aaccttttgt tagaatgggc ggaactgtcg gatctaatat taaggtttgg      780

cgtgacatta gtaacggtgc agatgagaaa atgctggata gagaagcaca ggccgcactt      840

gatttctcca gatatgtatt agaatgtcat aaacactcct ga                         882


<210>  4
<211>  888
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DPEase H10 de novo synthetized

<400>  4
catatgaaac atggtatata ctacgcatat tgggaacaag aatgggaagc tgattacaaa       60

tactatattg agaaggttgc aaagcttggt tttgatattc tagagattgc agcttcaccg      120

ctaccttttt acagtgacat tcagattaat gagctcaagg catgtgccca tggcaatgga      180

attacactta cggtaggcca tgggcctagt gcagaacaaa acctgtcttc tcccgacccc      240

gatattcgca aaaatgctaa agctttttat accgatttac tcaaacgact ttacaagctg      300

gatgtacatt tgataggtgg ggctttatat tcttattggc cgatagatta cacaaagaca      360

attgataaaa aaggcgattg ggaacgcagc gttgaaagtg ttcgagaagt tgctaaggtg      420

gccgaagcct gtggagtgga tttctgccta gaggttctta atagatttga gaattattta      480

attaacacag cacaagaggg tgtagatttt gtaaaacagg ttgaccataa caatgtaaag      540

gtaatgcttg ataccttcca catgaatatt gaggaagata gtatcggagg tgcaatcagg      600

actgcgggct cttacttggg acatttacac actggcgaat gtaatcgtaa agttcccggc      660

agaggaagaa ttccatgggt agaaattggt gaggctcttg ctgacatagg ttataacggt      720

agtgttgtta tggaaccttt tgttagaatg ggcggaactg tcggatctaa tattaaggtt      780

tggcgtgaca ttagtaacgg tgcagatgag aaaatgctgg atagagaagc acaggccgca      840

cttgatttct ccagatatgt attagaatgt cataaacact ccctcgag                   888


<210>  5
<211>  293
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  5

Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala 
1               5                   10                  15      


Asp Tyr Lys Tyr Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
            20                  25                  30          


Leu Glu Ile Ala Ala Ser Pro Leu Pro Phe Tyr Ser Asp Ile Gln Ile 
        35                  40                  45              


Asn Glu Leu Lys Ala Cys Ala His Gly Asn Gly Ile Thr Leu Thr Val 
    50                  55                  60                  


Gly His Gly Pro Ser Ala Glu Gln Asn Leu Ser Ser Pro Asp Pro Asp 
65                  70                  75                  80  


Ile Arg Lys Asn Ala Lys Ala Phe Tyr Thr Asp Leu Leu Lys Arg Leu 
                85                  90                  95      


Tyr Lys Leu Asp Val His Leu Ile Gly Gly Ala Leu Tyr Ser Tyr Trp 
            100                 105                 110         


Pro Ile Asp Tyr Thr Lys Thr Ile Asp Lys Lys Gly Asp Trp Glu Arg 
        115                 120                 125             


Ser Val Glu Ser Val Arg Glu Val Ala Lys Val Ala Glu Ala Cys Gly 
    130                 135                 140                 


Val Asp Phe Cys Leu Glu Val Leu Asn Arg Phe Glu Asn Tyr Leu Ile 
145                 150                 155                 160 


Asn Thr Ala Gln Glu Gly Val Asp Phe Val Lys Gln Val Asp His Asn 
                165                 170                 175     


Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
            180                 185                 190         


Ser Ile Gly Gly Ala Ile Arg Thr Ala Gly Ser Tyr Leu Gly His Leu 
        195                 200                 205             


His Thr Ser Glu Cys Asn Arg Lys Val Pro Gly Arg Gly Arg Ile Pro 
    210                 215                 220                 


Trp Val Glu Ile Gly Glu Ala Leu Ala Asp Ile Gly Tyr Asn Gly Ser 
225                 230                 235                 240 


Val Val Met Glu Pro Phe Val Arg Met Gly Gly Thr Val Gly Ser Asn 
                245                 250                 255     


Ile Lys Val Trp Arg Asp Ile Ser Asn Gly Ala Asp Glu Lys Met Leu 
            260                 265                 270         


Asp Arg Glu Ala Gln Ala Ala Leu Asp Phe Ser Arg Tyr Val Leu Glu 
        275                 280                 285             


Cys His Lys His Ser 
    290             


<210>  6
<211>  293
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  6

Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala 
1               5                   10                  15      


Asp Tyr Lys Tyr Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
            20                  25                  30          


Leu Glu Ile Ala Ala Ser Pro Leu Pro Phe Tyr Ser Asp Ile Gln Ile 
        35                  40                  45              


Asn Glu Leu Lys Ala Cys Ala His Gly Asn Gly Ile Thr Leu Thr Val 
    50                  55                  60                  


Gly His Gly Pro Ser Ala Glu Gln Asn Leu Ser Ser Pro Asp Pro Asp 
65                  70                  75                  80  


Ile Arg Lys Asn Ala Lys Ala Phe Tyr Thr Asp Leu Leu Lys Arg Leu 
                85                  90                  95      


Tyr Lys Leu Asp Val His Leu Ile Gly Gly Ala Leu Tyr Ser Tyr Trp 
            100                 105                 110         


Pro Ile Asp Tyr Thr Lys Thr Ile Asp Lys Lys Gly Asp Trp Glu Arg 
        115                 120                 125             


Ser Val Glu Ser Val Arg Glu Val Ala Lys Val Ala Glu Ala Cys Gly 
    130                 135                 140                 


Val Asp Phe Cys Leu Glu Val Leu Asn Arg Phe Glu Asn Tyr Leu Ile 
145                 150                 155                 160 


Asn Thr Ala Gln Glu Gly Val Asp Phe Val Lys Gln Val Asp His Asn 
                165                 170                 175     


Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
            180                 185                 190         


Ser Ile Gly Gly Ala Ile Arg Thr Ala Gly Ser Tyr Leu Gly His Leu 
        195                 200                 205             


His Thr Ser Glu Cys Asn Arg Lys Val Pro Gly Arg Gly Arg Ile Pro 
    210                 215                 220                 


Trp Val Glu Ile Gly Glu Ala Leu Ala Asp Ile Gly Tyr Asn Gly Ser 
225                 230                 235                 240 


Val Val Met Glu Pro Phe Val Arg Met Gly Gly Thr Val Gly Ser Asn 
                245                 250                 255     


Ile Lys Val Trp Arg Asp Ile Ser Asn Gly Ala Asp Glu Lys Met Leu 
            260                 265                 270         


Asp Arg Glu Ala Gln Ala Ala Leu Asp Phe Ser Arg Tyr Val Leu Glu 
        275                 280                 285             


Cys His Lys His Ser 
    290             


<210>  7
<211>  292
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  7

Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Glu Ala 
1               5                   10                  15      


Asp Tyr Lys Tyr Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
            20                  25                  30          


Leu Glu Ile Ala Ala Ser Pro Leu Pro Phe Tyr Ser Asp Asn Gln Ile 
        35                  40                  45              


Asn Glu Leu Lys Ala Cys Ala Arg Gly Asn Gly Ile Thr Leu Thr Val 
    50                  55                  60                  


Gly His Gly Pro Ser Ala Glu Gln Asn Leu Ser Ser Pro Asp Pro Tyr 
65                  70                  75                  80  


Ile Arg Lys Asn Ala Lys Ala Phe Tyr Thr Asp Leu Leu Lys Arg Leu 
                85                  90                  95      


Tyr Lys Leu Asp Val His Leu Ile Gly Gly Ala Ile Tyr Ser Tyr Trp 
            100                 105                 110         


Pro Val Asp Tyr Thr Lys Thr Ile Asp Lys Lys Gly Asp Trp Glu Arg 
        115                 120                 125             


Ser Val Glu Ser Val Arg Glu Val Ala Gln Val Ala Glu Ala Cys Gly 
    130                 135                 140                 


Val Asp Phe Cys Leu Glu Val Leu Asn Arg Phe Glu Asn Tyr Leu Ile 
145                 150                 155                 160 


Asn Thr Ala Gln Glu Gly Val Asp Phe Val Lys Gln Val Gly His Asp 
                165                 170                 175     


Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
            180                 185                 190         


Ser Ile Gly Gly Ala Ile Arg Thr Ala Gly Ser Tyr Leu Gly His Leu 
        195                 200                 205             


His Thr Ser Glu Cys Asn Arg Lys Val Pro Gly Lys Gly Arg Ile Pro 
    210                 215                 220                 


Trp Ile Glu Ile Gly Glu Ala Leu Ala Asp Ile Gly Tyr Asn Gly Ser 
225                 230                 235                 240 


Val Val Met Glu Pro Phe Val Arg Met Gly Gly Thr Val Gly Ser Asn 
                245                 250                 255     


Ile Lys Val Trp Arg Asp Ile Ser Asn Gly Ala Asp Glu Glu Lys Leu 
            260                 265                 270         


Asp Arg Glu Ala Gln Ala Ala Leu Asn Phe Ser Arg Tyr Val Leu Gly 
        275                 280                 285             


Asn Arg Lys Leu 
    290         


<210>  8
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  8

Met Lys His Gly Ile Tyr Tyr Ala Tyr Trp Glu Gln Glu Trp Ala Ala 
1               5                   10                  15      


Asp Tyr Lys Arg Tyr Val Glu Lys Ala Ala Lys Leu Gly Phe Asp Ile 
            20                  25                  30          


Leu Glu Val Gly Ala Ala Pro Leu Pro Asp Tyr Ser Ala Gln Glu Val 
        35                  40                  45              


Lys Glu Leu Lys Lys Cys Ala Asp Asp Asn Gly Ile Gln Leu Thr Ala 
    50                  55                  60                  


Gly Tyr Gly Pro Ala Phe Asn His Asn Met Gly Ser Ser Asp Pro Lys 
65                  70                  75                  80  


Ile Arg Glu Glu Ala Leu Gln Trp Tyr Lys Arg Leu Phe Glu Val Met 
                85                  90                  95      


Ala Gly Leu Asp Ile His Leu Ile Gly Gly Ala Leu Tyr Ser Tyr Trp 
            100                 105                 110         


Pro Val Asp Phe Ala Thr Ala Asn Lys Glu Glu Asp Trp Lys His Ser 
        115                 120                 125             


Val Glu Gly Met Gln Ile Leu Ala Pro Ile Ala Ser Gln Tyr Gly Ile 
    130                 135                 140                 


Asn Leu Gly Met Glu Val Leu Asn Arg Phe Glu Ser His Ile Leu Asn 
145                 150                 155                 160 


Thr Ser Glu Glu Gly Val Lys Phe Val Thr Glu Val Gly Met Asp Asn 
                165                 170                 175     


Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Ser Ser 
            180                 185                 190         


Ile Gly Asp Ala Ile Arg His Ala Gly Lys Leu Leu Gly His Phe His 
        195                 200                 205             


Thr Ser Glu Cys Asn Arg Met Val Pro Gly Lys Gly Arg Thr Pro Trp 
    210                 215                 220                 


Arg Glu Ile Gly Asp Ala Leu Arg Glu Ile Glu Tyr Asp Gly Thr Val 
225                 230                 235                 240 


Val Met Glu Pro Phe Val Arg Met Gly Gly Gln Val Gly Ser Asp Ile 
                245                 250                 255     


Lys Val Trp Arg Asp Ile Ser Lys Gly Ala Gly Glu Asp Arg Leu Asp 
            260                 265                 270         


Glu Asp Ala Arg Arg Ala Val Glu Phe Gln Arg Tyr Met Leu Glu Trp 
        275                 280                 285             


Lys 
    


<210>  9
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  9

Met Lys His Gly Ile Tyr Tyr Ser Tyr Trp Glu His Glu Trp Ser Ala 
1               5                   10                  15      


Lys Phe Gly Pro Tyr Ile Glu Lys Val Ala Lys Leu Gly Phe Asp Ile 
            20                  25                  30          


Ile Glu Val Ala Ala His His Ile Asn Glu Tyr Ser Asp Ala Glu Leu 
        35                  40                  45              


Ala Thr Ile Arg Lys Ser Ala Lys Asp Asn Gly Ile Ile Leu Thr Ala 
    50                  55                  60                  


Gly Ile Gly Pro Ser Lys Thr Lys Asn Leu Ser Ser Glu Asp Ala Ala 
65                  70                  75                  80  


Val Arg Ala Ala Gly Lys Ala Phe Phe Glu Arg Thr Leu Ser Asn Val 
                85                  90                  95      


Ala Lys Leu Asp Ile His Thr Ile Gly Gly Ala Leu His Ser Tyr Trp 
            100                 105                 110         


Pro Ile Asp Tyr Ser Gln Pro Val Asp Lys Ala Gly Asp Tyr Ala Arg 
        115                 120                 125             


Gly Val Glu Gly Ile Asn Gly Ile Ala Asp Phe Ala Asn Asp Leu Gly 
    130                 135                 140                 


Ile Asn Leu Cys Ile Glu Val Leu Asn Arg Phe Glu Asn His Val Leu 
145                 150                 155                 160 


Asn Thr Ala Ala Glu Gly Val Ala Phe Val Lys Asp Val Gly Lys Asn 
                165                 170                 175     


Asn Val Lys Val Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Asp 
            180                 185                 190         


Ser Phe Gly Asp Ala Ile Arg Thr Ala Gly Pro Leu Leu Gly His Phe 
        195                 200                 205             


His Thr Ser Glu Ser Asn Arg Arg Val Pro Gly Lys Gly Arg Met Pro 
    210                 215                 220                 


Trp His Glu Ile Gly Leu Ala Leu Arg Asp Ile Asn Tyr Thr Gly Ala 
225                 230                 235                 240 


Val Ile Met Glu Pro Phe Val Lys Thr Gly Gly Thr Ile Gly Ser Asp 
                245                 250                 255     


Ile Lys Val Trp Arg Asp Leu Ser Gly Gly Ala Asp Ile Ala Lys Met 
            260                 265                 270         


Asp Glu Asp Ala Arg Asn Ala Leu Ala Phe Ser Arg Phe Val Leu Gly 
        275                 280                 285             


<210>  10
<211>  291
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  10

Met Lys Tyr Gly Ile Tyr Tyr Ala Tyr Trp Glu Lys Glu Trp Asn Gly 
1               5                   10                  15      


Asp Tyr Lys Tyr Tyr Ile Asp Lys Ile Ser Lys Leu Gly Phe Asp Ile 
            20                  25                  30          


Leu Glu Ile Ser Cys Gly Ala Phe Ser Asp Tyr Tyr Thr Lys Asp Gln 
        35                  40                  45              


Glu Leu Ile Asp Ile Gly Lys Tyr Ala Lys Glu Lys Gly Val Thr Leu 
    50                  55                  60                  


Thr Ala Gly Tyr Gly Pro His Phe Asn Glu Ser Leu Ser Ser Ser Glu 
65                  70                  75                  80  


Pro Asn Thr Gln Lys Gln Ala Ile Ser Phe Trp Lys Glu Thr Leu Arg 
                85                  90                  95      


Lys Leu Lys Leu Met Asp Ile His Ile Val Gly Gly Ala Leu Tyr Gly 
            100                 105                 110         


Tyr Trp Pro Val Asp Tyr Ser Lys Pro Phe Asp Lys Lys Arg Asp Leu 
        115                 120                 125             


Glu Asn Ser Ile Lys Asn Met Lys Ile Ile Ser Gln Tyr Ala Glu Glu 
    130                 135                 140                 


Tyr Asp Ile Met Met Gly Met Glu Val Leu Asn Arg Phe Glu Gly Tyr 
145                 150                 155                 160 


Met Leu Asn Thr Cys Asp Glu Ala Leu Ala Tyr Val Glu Glu Val Gly 
                165                 170                 175     


Ser Ser Asn Val Gly Val Met Leu Asp Thr Phe His Met Asn Ile Glu 
            180                 185                 190         


Glu Asp Asn Ile Ala Ala Ala Ile Arg Lys Ala Gly Asp Arg Leu Tyr 
        195                 200                 205             


His Phe His Ile Ser Glu Gly Asn Arg Lys Val Pro Gly Lys Gly Met 
    210                 215                 220                 


Leu Pro Trp Asn Glu Ile Gly Gln Ala Leu Arg Asp Ile Asn Tyr Gln 
225                 230                 235                 240 


His Ala Ala Val Met Glu Pro Phe Val Met Gln Gly Gly Thr Val Gly 
                245                 250                 255     


His Asp Ile Lys Ile Trp Arg Asp Ile Ile Gly Asn Cys Ser Glu Val 
            260                 265                 270         


Thr Leu Asp Met Asp Ala Gln Ser Ala Leu His Phe Val Lys His Val 
        275                 280                 285             


Phe Glu Val 
    290     


<210>  11
<211>  301
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  11

Met Arg Tyr Phe Lys Glu Glu Val Ala Gly Met Lys Tyr Gly Ile Tyr 
1               5                   10                  15      


Phe Ala Tyr Trp Thr Lys Glu Trp Phe Ala Asp Tyr Lys Lys Tyr Met 
            20                  25                  30          


Asp Lys Val Ser Ala Leu Gly Phe Asp Val Leu Glu Ile Ser Cys Ala 
        35                  40                  45              


Ala Leu Arg Asp Val Tyr Thr Thr Lys Glu Gln Leu Ile Glu Leu Arg 
    50                  55                  60                  


Glu Tyr Ala Lys Glu Lys Gly Leu Val Leu Thr Ala Gly Tyr Gly Pro 
65                  70                  75                  80  


Thr Lys Ala Glu Asn Leu Cys Ser Glu Asp Pro Glu Ala Val Arg Arg 
                85                  90                  95      


Ala Met Thr Phe Phe Lys Asp Leu Leu Pro Lys Leu Gln Leu Met Asp 
            100                 105                 110         


Ile His Ile Leu Gly Gly Gly Leu Tyr Ser Tyr Trp Pro Val Asp Phe 
        115                 120                 125             


Thr Ile Asn Asn Asp Lys Gln Gly Asp Arg Ala Arg Ala Val Arg Asn 
    130                 135                 140                 


Leu Arg Glu Leu Ser Lys Thr Ala Glu Glu Cys Asp Val Val Leu Gly 
145                 150                 155                 160 


Met Glu Val Leu Asn Arg Tyr Glu Gly Tyr Ile Leu Asn Thr Cys Glu 
                165                 170                 175     


Glu Ala Ile Asp Phe Val Asp Glu Ile Gly Ser Ser His Val Lys Ile 
            180                 185                 190         


Met Leu Asp Thr Phe His Met Asn Ile Glu Glu Thr Asn Met Ala Asp 
        195                 200                 205             


Ala Ile Arg Lys Ala Gly Asp Arg Leu Gly His Leu His Leu Ser Glu 
    210                 215                 220                 


Gln Asn Arg Leu Val Pro Gly Lys Gly Ser Leu Pro Trp Ala Glu Ile 
225                 230                 235                 240 


Gly Gln Ala Leu Arg Asp Ile Asn Tyr Gln Gly Ala Ala Val Met Glu 
                245                 250                 255     


Pro Phe Val Met Gln Gly Gly Thr Ile Gly Ser Glu Ile Lys Val Trp 
            260                 265                 270         


Arg Asp Met Val Pro Asp Leu Ser Glu Glu Ala Leu Asp Arg Asp Ala 
        275                 280                 285             


Lys Gly Ala Leu Glu Phe Cys Arg His Val Phe Gly Ile 
    290                 295                 300     


<210>  12
<211>  290
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  12

Met Asn Lys Val Gly Met Phe Tyr Thr Tyr Trp Ser Thr Glu Trp Met 
1               5                   10                  15      


Val Asp Phe Pro Ala Thr Ala Lys Arg Ile Ala Gly Leu Gly Phe Asp 
            20                  25                  30          


Leu Met Glu Ile Ser Leu Gly Glu Phe His Asn Leu Ser Asp Ala Lys 
        35                  40                  45              


Lys Arg Glu Leu Lys Ala Val Ala Asp Asp Leu Gly Leu Thr Val Met 
    50                  55                  60                  


Cys Cys Ile Gly Leu Lys Ser Glu Tyr Asp Phe Ala Ser Pro Asp Lys 
65                  70                  75                  80  


Ser Val Arg Asp Ala Gly Thr Glu Tyr Val Lys Arg Leu Leu Asp Asp 
                85                  90                  95      


Cys His Leu Leu Gly Ala Pro Val Phe Ala Gly Leu Thr Phe Cys Ala 
            100                 105                 110         


Trp Pro Gln Ser Pro Pro Leu Asp Met Lys Asp Lys Arg Pro Tyr Val 
        115                 120                 125             


Asp Arg Ala Ile Glu Ser Val Arg Arg Val Ile Lys Val Ala Glu Asp 
    130                 135                 140                 


Tyr Gly Ile Ile Tyr Ala Leu Glu Val Val Asn Arg Phe Glu Gln Trp 
145                 150                 155                 160 


Leu Cys Asn Asp Ala Lys Glu Ala Ile Ala Phe Ala Asp Ala Val Asp 
                165                 170                 175     


Ser Pro Ala Cys Lys Val Gln Leu Asp Thr Phe His Met Asn Ile Glu 
            180                 185                 190         


Glu Thr Ser Phe Arg Asp Ala Ile Leu Ala Cys Lys Gly Lys Met Gly 
        195                 200                 205             


His Phe His Leu Ser Glu Ala Asn Arg Leu Pro Pro Gly Glu Gly Arg 
    210                 215                 220                 


Leu Pro Trp Asp Glu Ile Phe Gly Ala Leu Lys Glu Ile Gly Tyr Asp 
225                 230                 235                 240 


Gly Thr Ile Val Met Glu Pro Phe Met Arg Lys Gly Gly Ser Val Ser 
                245                 250                 255     


Arg Ala Val Gly Val Trp Arg Asp Met Ser Asn Gly Ala Thr Asp Glu 
            260                 265                 270         


Glu Met Asp Glu Arg Ala Arg Arg Ser Leu Gln Phe Val Arg Asp Lys 
        275                 280                 285             


Leu Ala 
    290 


<210>  13
<211>  295
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Artificial construct

<400>  13

Met Lys Asn Pro Val Gly Ile Ile Ser Met Gln Phe Ile Arg Pro Phe 
1               5                   10                  15      


Thr Ser Glu Ser Leu His Phe Leu Lys Lys Ser Arg Ala Leu Gly Phe 
            20                  25                  30          


Asp Phe Ile Glu Leu Leu Val Pro Glu Pro Glu Asp Gly Leu Asp Ala 
        35                  40                  45              


Ala Glu Val Arg Arg Ile Cys Glu Gly Glu Gly Leu Gly Leu Val Leu 
    50                  55                  60                  


Ala Ala Arg Val Asn Leu Gln Arg Ser Ile Ala Ser Glu Glu Ala Ala 
65                  70                  75                  80  


Ala Arg Ala Gly Gly Arg Asp Tyr Leu Lys Tyr Cys Ile Glu Ala Ala 
                85                  90                  95      


Glu Ala Leu Gly Ala Thr Ile Val Gly Gly Pro Leu Tyr Gly Glu Pro 
            100                 105                 110         


Leu Val Phe Ala Gly Arg Pro Pro Phe Pro Trp Thr Ala Glu Gln Ile 
        115                 120                 125             


Ala Thr Arg Ala Ala Arg Thr Val Glu Gly Leu Ala Glu Val Ala Pro 
    130                 135                 140                 


Leu Ala Ala Ser Ala Gly Lys Val Phe Gly Leu Glu Pro Leu Asn Arg 
145                 150                 155                 160 


Phe Glu Thr Asp Ile Val Asn Thr Thr Ala Gln Ala Ile Glu Val Val 
                165                 170                 175     


Asp Ala Val Gly Ser Pro Gly Leu Gly Val Met Leu Asp Thr Phe His 
            180                 185                 190         


Met Asn Met Glu Glu Arg Ser Ile Pro Asp Ala Ile Arg Ala Thr Gly 
        195                 200                 205             


Ala Arg Leu Val His Phe Gln Ala Asn Glu Asn His Arg Gly Phe Pro 
    210                 215                 220                 


Gly Thr Gly Thr Met Asp Trp Thr Ala Ile Ala Arg Ala Leu Gly Gln 
225                 230                 235                 240 


Ala Gly Tyr Ala Gly Pro Val Ser Leu Glu Pro Phe Arg Arg Asp Asp 
                245                 250                 255     


Glu Arg Val Ala Leu Pro Ile Ala His Trp Arg Ala Pro His Glu Asp 
            260                 265                 270         


Glu Asp Glu Lys Leu Arg Ala Gly Leu Gly Leu Ile Arg Ser Ala Ile 
        275                 280                 285             


Thr Leu Ala Glu Val Thr His 
    290                 295 


<210>  14
<211>  4681
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Plasmid pR1

<400>  14
cttaaggaac gtacagacgg cttaaaagcc tttaaaaacg tttttaaggg gtttgtagac       60

aaggtaaagg ataaaacagc acaattccaa gaaaaacacg atttagaacc taaaaagaac      120

gaatttgaac taactcataa ccgagaggta aaaaaagaac gaagtcgaga tcagggaatg      180

agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt tttgatggtt ttgaacttgt      240

tctttcttat cttgatacat atagaaataa cgtcattttt attttagttg ctgaaaggtg      300

cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa acccttaaaa ttggttgcac      360

agaaaaaccc catctgttaa agttataagt gactaaacaa ataactaaat agatgggggt      420

ttcttttaat attatgtgtc ctaatagtag catttattca gatgaaaaat caagggtttt      480

agtggacaag acaaaaagtg gaaaagtgag accatggaga gaaaagaaaa tcgctaatgt      540

tgattacttt gaacttctgc atattcttga atttaaaaag gctgaaagag taaaagattg      600

tgctgaaata ttagagtata aacaaaatcg tgaaacaggc gaaagaaagt tgtatcgagt      660

gtggttttgt aaatccaggc tttgtccaat gtgcaactgg aggagagcaa tgaaacatgg      720

cattcagtca caaaaggttg ttgctgaagt tattaaacaa aagccaacag ttcgttggtt      780

gtttctcaca ttaacagtta aaaatgttta tgatggcgaa gaattaaata agagtttgtc      840

agatatggct caaggatttc gccgaatgat gcaatataaa aaaattaata aaaatcttgt      900

tggttttatg cgtgcaacgg aagtgacaat aaataataaa gataattctt ataatcagca      960

catgcatgta ttggtatgtg tggaaccaac ttattttaag aatacagaaa actacgtgaa     1020

tcaaaaacaa tggattcaat tttggaaaaa ggcaatgaaa ttagactatg atccaaatgt     1080

aaaagttcaa atgattcgac cgaaaaataa atataaatcg gatatacaat cggcaattga     1140

cgaaactgca aaatatcctg taaaggatac ggattttatg accgatgatg aagaaaagaa     1200

tttgaaacgt ttgtctgatt tggaggaagg tttacaccgt aaaaggttaa tctcctatgg     1260

tggtttgtta aaagaaatac ataaaaaatt aaaccttgat gacacagaag aaggcgattt     1320

gattcataca gatgatgacg aaaaagccga tgaagatgga ttttctatta ttgcaatgtg     1380

gaattgggaa cggaaaaatt attttattaa agagtagttc aacaaacggg ccagtttgtt     1440

gaagattaga tgctataatt gttattaaaa ggattgaagg atgcttagga agacgagtta     1500

ttaatagctg aataagaacg gtgctctcca aatattctta tttagaaaag caaatctaaa     1560

attatctgaa aagggaagat ctttctaaag aggaaatggt gacagtagcg aaaagcatgc     1620

agggacaatc atcgaaataa ccgccaaagg ccaaacatga tttggccttt ttttcgttag     1680

acatcgtttc cctttagcct ttaattttag tatgatatgt aaatgatatt gaataaaagc     1740

taggaagtgt cgtaatgagc acaaaacctt tttacagaga tacgtgggcg gaaattgact     1800

tgtccgcgat aaaggaaaat gtcagcaata tgaaaaaaca tatcggtgaa catgtccact     1860

tgatggcagt tgtgaaagca aacgcctacg ggcatggtga tgcagaaaca gcaaaggctg     1920

ctcttgacgc aggtgcttca tgcttggccg tggccatttt ggatgaagcg atttcactgc     1980

gcaaaaaggg attgaaggcg cctatattgg tgcttggcgc ggttcccccg gagtatgtgg     2040

caatcgctgc tgagtatgac gtgaccttaa caggttattc tgttgaatgg cttcaggagg     2100

cagcccgcca cacgaaaaaa ggttctcttc attttcatct gaaggtcgat acggggatga     2160

acagacttgg tgtaaaaaca gaggaagaag ttcagaacgt gatggcaatt cttgaccgca     2220

accctcgttt aaagtgcaaa ggggtattta cccattttgc gacagcggat gaaaaagaaa     2280

gaggctattt cttaatgcag tttgagcgct ttaaagagct gattgctccg ctgccgttaa     2340

agaatctaat ggtccactgc gcgaacagcg ccgctggact ccggctgaaa aaaggctttt     2400

ttaatgcagt cagattcggc atcggcatgt atggccttcg cccgtctgct gacatgtcgg     2460

acgagatacc gtttcagctg cgtccggcat ttaccctgca ttcgacactg tcacatgtca     2520

aactgatcag aaaaggcgag agcgtcagct acggagccga gtacacagcg gaaaaagaca     2580

catggatcgg gacggtgcct gtaggctatg cggacggctg gctccgaaaa ttgaaaggga     2640

ccgacatcct tgtgaaggga aaacgcctga aaattgccgg ccgaatttgc atggaccaat     2700

ttatggtgga gctggatcag gaatatccgc cgggcacaaa agtcacatta ataggccggc     2760

agggggatga atatatttcc atggatgaga ttgcaggaag gctcgaaacc attaactatg     2820

aggtggcctg tacaataagt tcccgtgttc cccgtatgtt tttggaaaat gggagtataa     2880

tggaagtaag aaatccttta ttgcaggtaa atataagcaa ttaacttacc taaatggaga     2940

attcaatcta ttattaatct gttcagcaat cgggcgcgat tgctgaataa aagatacgag     3000

agacctctct tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa     3060

agacaataaa aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca     3120

aaataaaaat tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata     3180

ctacctgtcc cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt     3240

ggcagagggc aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt     3300

tttatggttt tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa     3360

gtatagtgtg ttatacttta cttggaagtg gttgccggaa agagcgaaaa tgcctcacat     3420

ttgtgccacc taaaaaggag cgatttacat atgagttatg cagtttgtag aatgcaaaaa     3480

gtgaaatcat aatgataggt ggtatgtttt cgcttgaact tttaaataca gccattgaac     3540

atacggttga tttaataact gacaaacatc accctcttgc taaagcggcc aaggacgctg     3600

ccgccggggc tgtttgcgtt tttgccgtga tttcgtgtat cattggttta cttatttttt     3660

tgccaaagct gtaatggctg aaaattctta catttatatt tacattttta gaaatgggcg     3720

tgaaaaaaag cgcgcgatta tgtaaaatat aaagtgatag cggtaccatt ataggtaaga     3780

gaggaatgta cacatgaaac atggtatata ctacgcatat tgggaacaag aatgggaagc     3840

tgattacaaa tactatattg agaaggttgc aaagcttggt tttgatattc tagagattgc     3900

agcttcaccg ctaccttttt acagtgacat tcagattaat gagctcaagg catgtgccca     3960

tggcaatgga attacactta cggtaggcca tgggcctagt gcagaacaaa acctgtcttc     4020

tcccgacccc gatattcgca aaaatgctaa agctttttat accgatttac tcaaacgact     4080

ttacaagctg gatgtacatt tgataggtgg ggctttatat tcttattggc cgatagatta     4140

cacaaagaca attgataaaa aaggcgattg ggaacgcagc gttgaaagtg ttcgagaagt     4200

tgctaaggtg gccgaagcct gtggagtgga tttctgccta gaggttctta atagatttga     4260

gaattattta attaacacag cacaagaggg tgtagatttt gtaaaacagg ttgaccataa     4320

caatgtaaag gtaatgcttg ataccttcca catgaatatt gaggaagata gtatcggagg     4380

tgcaatcagg actgcgggct cttacttggg acatttacac actggcgaat gtaatcgtaa     4440

agttcccggc agaggaagaa ttccatgggt agaaattggt gaggctcttg ctgacatagg     4500

ttataacggt agtgttgtta tggaaccttt tgttagaatg ggcggaactg tcggatctaa     4560

tattaaggtt tggcgtgaca ttagtaacgg tgcagatgag aaaatgctgg atagagaagc     4620

acaggccgca cttgatttct ccagatatgt attagaatgt cataaacact cctaagaatt     4680

c                                                                     4681


<210>  15
<211>  4681
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Plasmid pR2

<400>  15
cttaaggaac gtacagacgg cttaaaagcc tttaaaaacg tttttaaggg gtttgtagac       60

aaggtaaagg ataaaacagc acaattccaa gaaaaacacg atttagaacc taaaaagaac      120

gaatttgaac taactcataa ccgagaggta aaaaaagaac gaagtcgaga tcagggaatg      180

agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt tttgatggtt ttgaacttgt      240

tctttcttat cttgatacat atagaaataa cgtcattttt attttagttg ctgaaaggtg      300

cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa acccttaaaa ttggttgcac      360

agaaaaaccc catctgttaa agttataagt gactaaacaa ataactaaat agatgggggt      420

ttcttttaat attatgtgtc ctaatagtag catttattca gatgaaaaat caagggtttt      480

agtggacaag acaaaaagtg gaaaagtgag accatggaga gaaaagaaaa tcgctaatgt      540

tgattacttt gaacttctgc atattcttga atttaaaaag gctgaaagag taaaagattg      600

tgctgaaata ttagagtata aacaaaatcg tgaaacaggc gaaagaaagt tgtatcgagt      660

gtggttttgt aaatccaggc tttgtccaat gtgcaactgg aggagagcaa tgaaacatgg      720

cattcagtca caaaaggttg ttgctgaagt tattaaacaa aagccaacag ttcgttggtt      780

gtttctcaca ttaacagtta aaaatgttta tgatggcgaa gaattaaata agagtttgtc      840

agatatggct caaggatttc gccgaatgat gcaatataaa aaaattaata aaaatcttgt      900

tggttttatg cgtgcaacgg aagtgacaat aaataataaa gataattctt ataatcagca      960

catgcatgta ttggtatgtg tggaaccaac ttattttaag aatacagaaa actacgtgaa     1020

tcaaaaacaa tggattcaat tttggaaaaa ggcaatgaaa ttagactatg atccaaatgt     1080

aaaagttcaa atgattcgac cgaaaaataa atataaatcg gatatacaat cggcaattga     1140

cgaaactgca aaatatcctg taaaggatac ggattttatg accgatgatg aagaaaagaa     1200

tttgaaacgt ttgtctgatt tggaggaagg tttacaccgt aaaaggttaa tctcctatgg     1260

tggtttgtta aaagaaatac ataaaaaatt aaaccttgat gacacagaag aaggcgattt     1320

gattcataca gatgatgacg aaaaagccga tgaagatgga ttttctatta ttgcaatgtg     1380

gaattgggaa cggaaaaatt attttattaa agagtagttc aacaaacggg ccagtttgtt     1440

gaagattaga tgctataatt gttattaaaa ggattgaagg atgcttagga agacgagtta     1500

ttaatagctg aataagaacg gtgctctcca aatattctta tttagaaaag caaatctaaa     1560

attatctgaa aagggaagat ctttctaaag aggaaatggt gacagtagcg aaaagcatgc     1620

agggacaatc atcgaaataa ccgccaaagg ccaaacatga tttggccttt ttttcgttag     1680

acatcgtttc cctttagcct ttaattttag tatgatatgt aaatgatatt gaataaaagc     1740

taggaagtgt cgtaatgagc acaaaacctt tttacagaga tacgtgggcg gaaattgact     1800

tgtccgcgat aaaggaaaat gtcagcaata tgaaaaaaca tatcggtgaa catgtccact     1860

tgatggcagt tgtgaaagca aacgcctacg ggcatggtga tgcagaaaca gcaaaggctg     1920

ctcttgacgc aggtgcttca tgcttggccg tggccatttt ggatgaagcg atttcactgc     1980

gcaaaaaggg attgaaggcg cctatattgg tgcttggcgc ggttcccccg gagtatgtgg     2040

caatcgctgc tgagtatgac gtgaccttaa caggttattc tgttgaatgg cttcaggagg     2100

cagcccgcca cacgaaaaaa ggttctcttc attttcatct gaaggtcgat acggggatga     2160

acagacttgg tgtaaaaaca gaggaagaag ttcagaacgt gatggcaatt cttgaccgca     2220

accctcgttt aaagtgcaaa ggggtattta cccattttgc gacagcggat gaaaaagaaa     2280

gaggctattt cttaatgcag tttgagcgct ttaaagagct gattgctccg ctgccgttaa     2340

agaatctaat ggtccactgc gcgaacagcg ccgctggact ccggctgaaa aaaggctttt     2400

ttaatgcagt cagattcggc atcggcatgt atggccttcg cccgtctgct gacatgtcgg     2460

acgagatacc gtttcagctg cgtccggcat ttaccctgca ttcgacactg tcacatgtca     2520

aactgatcag aaaaggcgag agcgtcagct acggagccga gtacacagcg gaaaaagaca     2580

catggatcgg gacggtgcct gtaggctatg cggacggctg gctccgaaaa ttgaaaggga     2640

ccgacatcct tgtgaaggga aaacgcctga aaattgccgg ccgaatttgc atggaccaat     2700

ttatggtgga gctggatcag gaatatccgc cgggcacaaa agtcacatta ataggccggc     2760

agggggatga atatatttcc atggatgaga ttgcaggaag gctcgaaacc attaactatg     2820

aggtggcctg tacaataagt tcccgtgttc cccgtatgtt tttggaaaat gggagtataa     2880

tggaagtaag aaatccttta ttgcaggtaa atataagcaa ttaacttacc taaatggaga     2940

attcaatcta ttattaatct gttcagcaat cgggcgcgat tgctgaataa aagatacgag     3000

agacctctct tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa     3060

agacaataaa aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca     3120

aaataaaaat tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata     3180

ctacctgtcc cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt     3240

ggcagagggc aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt     3300

tttatggttt tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa     3360

gtatagtgtg ttatacttta cttggaagtg gttgccggaa agagcgaaaa tgcctcacat     3420

ttgtgccacc taaaaaggag cgatttacat atgagttatg cagtttgtag aatgcaaaaa     3480

gtgaaatcat aatgataggt ggtatgtttt cgcttgaact tttaaataca gccattgaac     3540

atacggttga tttaataact gacaaacatc accctcttgc taaagcggcc aaggacgctg     3600

ccgccggggc tgtttgcgtt tttgccgtga tttcgtgtat cattggttta cttatttttt     3660

tgccaaagct gtaatggctg aaaattctta catttatatt tacattttta gaaatgggcg     3720

tgaaaaaaag cgcgcgatta tgtaaaatat aaagtgatag cggtaccatt ataggtagaa     3780

aggaggatta catatgaaac atggtatata ctacgcatat tgggaacaag aatgggaagc     3840

tgattacaaa tactatattg agaaggttgc aaagcttggt tttgatattc tagagattgc     3900

agcttcaccg ctaccttttt acagtgacat tcagattaat gagctcaagg catgtgccca     3960

tggcaatgga attacactta cggtaggcca tgggcctagt gcagaacaaa acctgtcttc     4020

tcccgacccc gatattcgca aaaatgctaa agctttttat accgatttac tcaaacgact     4080

ttacaagctg gatgtacatt tgataggtgg ggctttatat tcttattggc cgatagatta     4140

cacaaagaca attgataaaa aaggcgattg ggaacgcagc gttgaaagtg ttcgagaagt     4200

tgctaaggtg gccgaagcct gtggagtgga tttctgccta gaggttctta atagatttga     4260

gaattattta attaacacag cacaagaggg tgtagatttt gtaaaacagg ttgaccataa     4320

caatgtaaag gtaatgcttg ataccttcca catgaatatt gaggaagata gtatcggagg     4380

tgcaatcagg actgcgggct cttacttggg acatttacac actggcgaat gtaatcgtaa     4440

agttcccggc agaggaagaa ttccatgggt agaaattggt gaggctcttg ctgacatagg     4500

ttataacggt agtgttgtta tggaaccttt tgttagaatg ggcggaactg tcggatctaa     4560

tattaaggtt tggcgtgaca ttagtaacgg tgcagatgag aaaatgctgg atagagaagc     4620

acaggccgca cttgatttct ccagatatgt attagaatgt cataaacact cctaagaatt     4680

c                                                                     4681


<210>  16
<211>  4681
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Plasmid pR3

<400>  16
cttaaggaac gtacagacgg cttaaaagcc tttaaaaacg tttttaaggg gtttgtagac       60

aaggtaaagg ataaaacagc acaattccaa gaaaaacacg atttagaacc taaaaagaac      120

gaatttgaac taactcataa ccgagaggta aaaaaagaac gaagtcgaga tcagggaatg      180

agtttataaa ataaaaaaag cacctgaaaa ggtgtctttt tttgatggtt ttgaacttgt      240

tctttcttat cttgatacat atagaaataa cgtcattttt attttagttg ctgaaaggtg      300

cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa acccttaaaa ttggttgcac      360

agaaaaaccc catctgttaa agttataagt gactaaacaa ataactaaat agatgggggt      420

ttcttttaat attatgtgtc ctaatagtag catttattca gatgaaaaat caagggtttt      480

agtggacaag acaaaaagtg gaaaagtgag accatggaga gaaaagaaaa tcgctaatgt      540

tgattacttt gaacttctgc atattcttga atttaaaaag gctgaaagag taaaagattg      600

tgctgaaata ttagagtata aacaaaatcg tgaaacaggc gaaagaaagt tgtatcgagt      660

gtggttttgt aaatccaggc tttgtccaat gtgcaactgg aggagagcaa tgaaacatgg      720

cattcagtca caaaaggttg ttgctgaagt tattaaacaa aagccaacag ttcgttggtt      780

gtttctcaca ttaacagtta aaaatgttta tgatggcgaa gaattaaata agagtttgtc      840

agatatggct caaggatttc gccgaatgat gcaatataaa aaaattaata aaaatcttgt      900

tggttttatg cgtgcaacgg aagtgacaat aaataataaa gataattctt ataatcagca      960

catgcatgta ttggtatgtg tggaaccaac ttattttaag aatacagaaa actacgtgaa     1020

tcaaaaacaa tggattcaat tttggaaaaa ggcaatgaaa ttagactatg atccaaatgt     1080

aaaagttcaa atgattcgac cgaaaaataa atataaatcg gatatacaat cggcaattga     1140

cgaaactgca aaatatcctg taaaggatac ggattttatg accgatgatg aagaaaagaa     1200

tttgaaacgt ttgtctgatt tggaggaagg tttacaccgt aaaaggttaa tctcctatgg     1260

tggtttgtta aaagaaatac ataaaaaatt aaaccttgat gacacagaag aaggcgattt     1320

gattcataca gatgatgacg aaaaagccga tgaagatgga ttttctatta ttgcaatgtg     1380

gaattgggaa cggaaaaatt attttattaa agagtagttc aacaaacggg ccagtttgtt     1440

gaagattaga tgctataatt gttattaaaa ggattgaagg atgcttagga agacgagtta     1500

ttaatagctg aataagaacg gtgctctcca aatattctta tttagaaaag caaatctaaa     1560

attatctgaa aagggaagat ctttctaaag aggaaatggt gacagtagcg aaaagcatgc     1620

agggacaatc atcgaaataa ccgccaaagg ccaaacatga tttggccttt ttttcgttag     1680

acatcgtttc cctttagcct ttaattttag tatgatatgt aaatgatatt gaataaaagc     1740

taggaagtgt cgtaatgagc acaaaacctt tttacagaga tacgtgggcg gaaattgact     1800

tgtccgcgat aaaggaaaat gtcagcaata tgaaaaaaca tatcggtgaa catgtccact     1860

tgatggcagt tgtgaaagca aacgcctacg ggcatggtga tgcagaaaca gcaaaggctg     1920

ctcttgacgc aggtgcttca tgcttggccg tggccatttt ggatgaagcg atttcactgc     1980

gcaaaaaggg attgaaggcg cctatattgg tgcttggcgc ggttcccccg gagtatgtgg     2040

caatcgctgc tgagtatgac gtgaccttaa caggttattc tgttgaatgg cttcaggagg     2100

cagcccgcca cacgaaaaaa ggttctcttc attttcatct gaaggtcgat acggggatga     2160

acagacttgg tgtaaaaaca gaggaagaag ttcagaacgt gatggcaatt cttgaccgca     2220

accctcgttt aaagtgcaaa ggggtattta cccattttgc gacagcggat gaaaaagaaa     2280

gaggctattt cttaatgcag tttgagcgct ttaaagagct gattgctccg ctgccgttaa     2340

agaatctaat ggtccactgc gcgaacagcg ccgctggact ccggctgaaa aaaggctttt     2400

ttaatgcagt cagattcggc atcggcatgt atggccttcg cccgtctgct gacatgtcgg     2460

acgagatacc gtttcagctg cgtccggcat ttaccctgca ttcgacactg tcacatgtca     2520

aactgatcag aaaaggcgag agcgtcagct acggagccga gtacacagcg gaaaaagaca     2580

catggatcgg gacggtgcct gtaggctatg cggacggctg gctccgaaaa ttgaaaggga     2640

ccgacatcct tgtgaaggga aaacgcctga aaattgccgg ccgaatttgc atggaccaat     2700

ttatggtgga gctggatcag gaatatccgc cgggcacaaa agtcacatta ataggccggc     2760

agggggatga atatatttcc atggatgaga ttgcaggaag gctcgaaacc attaactatg     2820

aggtggcctg tacaataagt tcccgtgttc cccgtatgtt tttggaaaat gggagtataa     2880

tggaagtaag aaatccttta ttgcaggtaa atataagcaa ttaacttacc taaatggaga     2940

attcaatcta ttattaatct gttcagcaat cgggcgcgat tgctgaataa aagatacgag     3000

agacctctct tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa     3060

agacaataaa aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca     3120

aaataaaaat tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata     3180

ctacctgtcc cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt     3240

ggcagagggc aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt     3300

tttatggttt tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa     3360

gtatagtgtg ttatacttta cttggaagtg gttgccggaa agagcgaaaa tgcctcacat     3420

ttgtgccacc taaaaaggag cgatttacat atgagttatg cagtttgtag aatgcaaaaa     3480

gtgaaatcat aatgataggt ggtatgtttt cgcttgaact tttaaataca gccattgaac     3540

atacggttga tttaataact gacaaacatc accctcttgc taaagcggcc aaggacgctg     3600

ccgccggggc tgtttgcgtt tttgccgtga tttcgtgtat cattggttta cttatttttt     3660

tgccaaagct gtaatggctg aaaattctta catttatatt tacattttta gaaatgggcg     3720

tgaaaaaaag cgcgcgatta tgtaaaatat aaagtgatag cggtaccatt ataggtagaa     3780

aggaggattc gaaatgaaac atggtatata ctacgcatat tgggaacaag aatgggaagc     3840

tgattacaaa tactatattg agaaggttgc aaagcttggt tttgatattc tagagattgc     3900

agcttcaccg ctaccttttt acagtgacat tcagattaat gagctcaagg catgtgccca     3960

tggcaatgga attacactta cggtaggcca tgggcctagt gcagaacaaa acctgtcttc     4020

tcccgacccc gatattcgca aaaatgctaa agctttttat accgatttac tcaaacgact     4080

ttacaagctg gatgtacatt tgataggtgg ggctttatat tcttattggc cgatagatta     4140

cacaaagaca attgataaaa aaggcgattg ggaacgcagc gttgaaagtg ttcgagaagt     4200

tgctaaggtg gccgaagcct gtggagtgga tttctgccta gaggttctta atagatttga     4260

gaattattta attaacacag cacaagaggg tgtagatttt gtaaaacagg ttgaccataa     4320

caatgtaaag gtaatgcttg ataccttcca catgaatatt gaggaagata gtatcggagg     4380

tgcaatcagg actgcgggct cttacttggg acatttacac actggcgaat gtaatcgtaa     4440

agttcccggc agaggaagaa ttccatgggt agaaattggt gaggctcttg ctgacatagg     4500

ttataacggt agtgttgtta tggaaccttt tgttagaatg ggcggaactg tcggatctaa     4560

tattaaggtt tggcgtgaca ttagtaacgg tgcagatgag aaaatgctgg atagagaagc     4620

acaggccgca cttgatttct ccagatatgt attagaatgt cataaacact cctaagaatt     4680

c                                                                     4681


<210>  17
<211>  1170
<212>  DNA
<213>  Bacillus subtilis

<400>  17
atgagcacaa aaccttttta cagagatacg tgggcggaaa ttgacttgtc cgcgataaag       60

gaaaatgtca gcaatatgaa aaaacatatc ggtgaacatg tccacttgat ggcagttgtg      120

aaagcaaacg cctacgggca tggtgatgca gaaacagcaa aggctgctct tgacgcaggt      180

gcttcatgct tggccgtggc cattttggat gaagcgattt cactgcgcaa aaagggattg      240

aaggcgccta tattggtgct tggcgcggtt cccccggagt atgtggcaat cgctgctgag      300

tatgacgtga ccttaacagg ttattctgtt gaatggcttc aggaggcagc ccgccacacg      360

aaaaaaggtt ctcttcattt tcatctgaag gtcgatacgg ggatgaacag acttggtgta      420

aaaacagagg aagaagttca gaacgtgatg gcaattcttg accgcaaccc tcgtttaaag      480

tgcaaagggg tatttaccca ttttgcgaca gcggatgaaa aagaaagagg ctatttctta      540

atgcagtttg agcgctttaa agagctgatt gctccgctgc cgttaaagaa tctaatggtc      600

cactgcgcga acagcgccgc tggactccgg ctgaaaaaag gcttttttaa tgcagtcaga      660

ttcggcatcg gcatgtatgg ccttcgcccg tctgctgaca tgtcggacga gataccgttt      720

cagctgcgtc cggcatttac cctgcattcg acactgtcac atgtcaaact gatcagaaaa      780

ggcgagagcg tcagctacgg agccgagtac acagcggaaa aagacacatg gatcgggacg      840

gtgcctgtag gctatgcgga cggctggctc cgaaaattga aagggaccga catccttgtg      900

aagggaaaac gcctgaaaat tgccggccga atttgcatgg accaatttat ggtggagctg      960

gatcaggaat atccgccggg cacaaaagtc acattaatag gccggcaggg ggatgaatat     1020

atttccatgg atgagattgc aggaaggctc gaaaccatta actatgaggt ggcctgtaca     1080

ataagttccc gtgttccccg tatgtttttg gaaaatggga gtataatgga agtaagaaat     1140

cctttattgc aggtaaatat aagcaattaa                                      1170


<210>  18
<211>  1197
<212>  DNA
<213>  Bacillus subtilis

<400>  18
gtgaaaaata aatggctgtc ttttttttcg ggtaaggtcc agcttgaatt gacgggaaga       60

gggattgagc ggctccttaa tgaatgcaca agacagggga ttccggtctt tcatgtcaaa      120

aaaaagaaag aagccgtatc gttatatata cagcttcagg atgtacatgc ctttcggcgg      180

gtaagaagta aatttaaatg taaagcccga tttatcaatc ggaagggatt tcccttcctg      240

ttgctgaaat caaagctgaa tatagggttt acgatcggtt ttgcgatttt tttcattctt      300

ttgtttttgc tgtccaatat ggtgtggaaa attgatgtga caggcgctaa gcctgaaaca      360

gaacatcaaa tgaggcagca tcttaatgaa atcggcgtca aaaagggccg tctgcagttt      420

ttaatgatgt cgcccgaaaa aatacagaaa tcattaacca atggaataga caatatcact      480

tgggtcggag ttgatctgaa ggggacgacc attcatatga aagttgtgga gaaaaatgag      540

cccgaaaaag aaaaatatgt tagcccgcgc aatattgtcg ccaaaaagaa agcaaccatt      600

acgagaatgt ttgtgcaaaa aggacagccc atggccgcca tacacgatca tgttgaaaag      660

ggacagctgc ttgtttcggg actgatcggc agcgaagacc atcagcagga agtcgcctca      720

aaagcagaaa tttatggaga aacctggtat agatcagaag tgacagtccc gcttgaaaca      780

ttatttaacg tctatacggg caaagtaagg acaaagcaca agctttcttt tggttctttg      840

gcaatcccga tctgggggat gacgtttaaa aaagaggaat tgaagcatcc aaaaacagaa      900

caagaaaagc attcgcttca ttttctcgga tttaagctcc ctgtatccta tgtcaaagag      960

caaacgagag aaagtgaaga ggctttgcga aaatatacaa aagaagaagc agttcaagaa     1020

ggcattaaat tgggtaaaca ggatgtagag gataaaatag gcgaaaacgg cgaggtgaaa     1080

agtgaaaaag ttttgcacca gactgttgag aatggtaaag taaagttgat tattctctac     1140

caagttatag aagatatcgt tcaaaccaca cctattgtca gggagactga agaatga        1197


<210>  19
<211>  4579
<212>  DNA
<213>  Bacillus subtilis

<400>  19
tgacaatatg tctcctgtca ttatgtcctt cacactctga tcaaacgtga ccagctgttt       60

ttcttccgtg aaattcatga caaaaatata atcattgtcc tgatcctgcc tcgcttgtac      120

ggagacgcct tttccgtgcc gaaccggaaa aactggagag agagacaggt ctgtgatcag      180

accctcatag aaatcacgct gaaattgatc ctccaaacgc gcgccgataa aatacgcctt      240

gccctgctga tactcatggc ttgtgaccgc tggcgtgcgc gcataaaaat cttcttgata      300

caccgcttcc actgaagctg tctttacatc aatcacggtt gcataatcct tcatttcata      360

tatttggctg cggtagctga cagcgtttcg atccttcgga tacagggtgt ccgtttcaag      420

aggctcaact ccaaatatag cttgaaatcg atatctctgc agtcgcgatg attaattaat      480

tcagaacgct cggttgccgc cgggcgtttt ttatgcagca atggcaagaa cgtcccgggg      540

agctcctaac ttataggggt aacacttaaa aaagaatcaa taacgataga aaccgctcct      600

aaagcaggtg cattttttcc taacgaagaa ggcaatagtt cacatttatt gtctaaatga      660

gaatggactc tagaagaaac ttcgttttta atcgtattta aaacaatggg atgagattca      720

attatatgat ttctcaagat aacagcttct atatcaaatg tattaaggat attggttaat      780

ccaattccga tataaaagcc aaagttttga agtgcattta acatttctac atcattttta      840

tttgcgcgtt ccacaatctc ttttcgagaa atattctttt cttctttaga gagcgaagcc      900

agtaacgctt tttcagaagc atataattcc caacagcctc gatttccaca gctgcatttg      960

ggtccattaa aatctatcgt catatgaccc atttccccag aaaaaccctg aacaccttta     1020

tacaattcgt tgttaataac aagtccagtt ccaattccga tattaatact gatgtaaacg     1080

atgttttcat agttttttgt cataccaaat actttttcac cgtatgctcc tgcattagct     1140

tcattttcaa caaaaaccgg aacattaaac tcactctcaa ttaaaaactg caaatctttg     1200

atattccaat ttaagttagg catgaaaata atttgctgat gacgatctac aaggcctgga     1260

acacaaattc ctattccgac tagaccataa ggggactcag gcatatgggt tacaaaacca     1320

tgaataagtg caaataaaat ctcttttact tcactagcgg aagaactaga caagtcagaa     1380

gtcttctcga gaataatatt tccttctaag tcggttagaa ttccgttaag atagtcgact     1440

cctatatcaa taccaatcga gtagcctgca ttcttattaa aaacaagcat tacaggtctt     1500

ctgccgcctc tagattgccc tgccccaatt tcaaaaataa aatctttttc aagcagtgta     1560

tttacttgag aggagacagt agacttgttt aatcctgtaa tctcagagag agttgccctg     1620

gagacagggg agttcttcaa aatttcatct aatattaatt tttgattcat tttttttact     1680

aaagcttgat ctgcaatttg aataataacc actcctttgt ttatccaccg aactaagttg     1740

gtgttttttg aagcttgaat tagatattta aaagtatcat atctaatatt ataactaaat     1800

tttctaaaaa aaacattgaa ataaacattt attttgtata tgatgagata aagttagttt     1860

attggataaa caaactaact caattaagat agttgatgga taaacttgtt cacttaaatc     1920

aaagggggaa atgacaaatg gtccaaacta gtgatatcta aaaatcaaag ggggaaatgg     1980

gatccaaagg aggccataat atgagtcaga aaacagacgc acctttagaa tcgtatgaag     2040

tgaacggcgc aacaattgcc gtgctgccag aagaaataga cggcaaaatc tgttccaaaa     2100

ttattgaaaa agattgcgtg ttttatgtaa acatgaagcc gctgcaaatt gtcgacagaa     2160

gctgccgatt ttttggatca agctatgcgg gaagaaaagc aggaacttat gaagtgacaa     2220

aaatttcaca caagccgccg atcatggtgg acccttcgaa ccaaatcttt ttattcccta     2280

cactttcttc gacaagaccc caatgcggct ggatttccca tgtgcatgta aaagaattca     2340

aagcgactga attcgacgat acggaagtga cgttttccaa tgggaaaacg atggagctgc     2400

cgatctctta taattcgttc gagaaccagg tataccgaac agcgtggctc agaaccaaat     2460

tccaagacag aatcgaccac cgcgtgccga aaagacagga atttatgctg tacccgaaag     2520

aagagcggac gaagatgatt tatgatttta ttttgcgtga gctcggggaa cggtattaga     2580

aaaatagccg cgggcggccg cactcttcct ttttcaatat tattgaagca tttatcaggg     2640

ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt     2700

tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac     2760

attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tgatcctcta     2820

gcacaaaaag aaaaacgaaa tgatacacca atcagtgcaa aaaaagatat aatgggagat     2880

aagacggttc gtgttcgtgc tgacttgcac catatcataa aaatcgaaac agcaaagaat     2940

ggcggaaacg taaaagaagt tatggaaata agacttagaa gcaaacttaa gagtgtgttg     3000

atagtgcagt atcttaaaat tttgtataat aggaattgaa gttaaattag atgctaaaaa     3060

tttgtaatta agaaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt     3120

aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac     3180

cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa     3240

acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa     3300

actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa     3360

acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa ttattaaaaa     3420

agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa     3480

gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca     3540

gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt     3600

aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta     3660

ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca     3720

tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt     3780

gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt     3840

gtaaatttgg aaagttacac gttactaaag ggaatgtaga taaattatta ggtatactac     3900

tgacagcttc caaggagcta aagaggtccc tagactctag acccggggat ctctgcagtc     3960

gggaagatct ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg     4020

aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca     4080

aatccgccgc tctagctaag cagaaggcca tcctgacgga tggccttttt gcgtttctac     4140

aaactcttgt taactctaga gctgcctgcc gcgtttcggt gatgaagatc ttcccgatga     4200

ttaattaatt cagaacgctc ggttgccgcc gggcgttttt tatgcagcaa tggcaagaac     4260

gttgctctag agcggccgca tcgattcaca gtggcaatct cccccgtatt cgtttgaaat     4320

gtgccacatt aacagcgccg ggtgatgtcc gtatcgttct gctaataagc ggttgatgtg     4380

ccgtgttttt tctcggtaga ctttagatgt gaggcagtgg ttgtgccttc cgccgtgcag     4440

ctgtttgacg cgggaggcat tgacgcgcaa aacttccgga taggtttgcg acagccaggc     4500

cggacgggct ccgctcggcg ttgctaatat gacccggccg cctatactgt gaatccgctc     4560

aaaaatatca tccagccat                                                  4579


<210>  20
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  20
ttaccttctc tcttctaagt accgttcgta tagcat                                 36


<210>  21
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  21
caagcaaagc tgttttatct accgttcgta taatgt                                 36


<210>  22
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  22
tacaaagcaa aagcgaaaat gaccatc                                           27


<210>  23
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  23
atgctatacg aacggtactt agaagagaga aggtaa                                 36


<210>  24
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  24
acattatacg aacggtagat aaaacagctt tgcttg                                 36


<210>  25
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  25
cagctgatag gattcttgct cgctta                                            26


<210>  26
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  26
tgataggtgg tatgttttcg ctt                                               23


<210>  27
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  27
ataaatacca tgcttcatgt gtacattcct ctctta                                 36


<210>  28
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  28
taagagagga atgtacacat gaaacatggt atatac                                 36


<210>  29
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  29
gaattcttag gagtgtttat gacattc                                           27


<210>  30
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  30
tagaatgcaa aaagtgaaat cataatgata ggtggtatgt tttcgcttga                  50


<210>  31
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  31
cgtctgtacg ttccttaagg aattcttagg agtgtttatg acattctaat                  50


<210>  32
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  32
attagaatgt cataaacact cctaagaatt ccttaaggaa cgtacagacg                  50


<210>  33
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  33
tcaagcgaaa acataccacc tatcattatg atttcacttt ttgcatt                     47


<210>  34
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  34
aaatctaaaa ttatctgaaa agggaagatc tttctaaaga ggaaatggtg                  50


<210>  35
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  35
ttgctgaaca gattaataat agattgaatt ctccatttag gtaagttaat                  50


<210>  36
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  36
attaacttac ctaaatggag aattcaatct attattaatc tgttcagcaa                  50


<210>  37
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  37
caccatttcc tctttagaaa gatcttccct tttcagataa ttttagattt                  50


<210>  38
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetized 5'UTR of the DPEase

<400>  38
agcggtacca ttataggtaa gagaggaatg tacacatgaa acatggtata tactacgcat       60

attgg                                                                   65


<210>  39
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetized 5'UTR of the DPEase

<400>  39
agcggtacca ttataggtag aaaggaggat tacatatgaa acatggtata tactacgcat       60

attgg                                                                   65


<210>  40
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetized 5'UTR of the DPEase

<400>  40
agcggtacca ttataggtag aaaggaggat tcgaaatgaa acatggtata tactacgcat       60

attgg                                                                   65


<210>  41
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  41
agagaggaat gtacac                                                       16


<210>  42
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  42
gaaaggagga ttcgaa                                                       16


<210>  43
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  43
gaaaggagga ttatgg                                                       16


<210>  44
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  44
gaaaggagga ttgtcg                                                       16


<210>  45
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  45
gaaaggagga tttagt                                                       16


<210>  46
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  46
gaaaggagga ttgagg                                                       16


<210>  47
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  47
agaaaggagg attaaa                                                       16


<210>  48
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  48
gaaaggagga tttcgt                                                       16


<210>  49
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'UTR of the DPEase gene

<400>  49
gaaaggagga tttttg                                                       16


