﻿                         序列表

<110>  江南大学

<120>  热稳定性提升的角蛋白酶突变体及其应用

<150>  2017110581635
<151>  2017-11-01

<160>  14

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  275
<212>  PRT
<213>  （人工序列）

<400>  1
Ala Gln Thr Val Pro Tyr Gly Ile Pro Gln Ile Lys Ala Pro Ala Val 
1               5                   10                  15      
His Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile His Ala Ala His Pro Asp Leu Asn Val Ala Gly Gly Ala 
        35                  40                  45              
Ser Phe Val Pro Ser Glu Pro Asn Ala Thr Gln Asp Phe Gln Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asp Asn Thr Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Arg Tyr Gly Asp Gly Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu 
            100                 105                 110         
Trp Ala Val Ala Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Ser Gly Ser Thr Ala Leu Lys Asn Thr Val Asp Thr Ala Asn Asn 
    130                 135                 140                 
Arg Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Ser Ser Thr Val Gly Tyr Pro Ala Lys Tyr Asp Ser Thr Ile Ala 
                165                 170                 175     
Val Ala Asn Val Asp Ser Asn Asn Val Arg Asn Ser Ser Ser Ser Ala 
            180                 185                 190         
Gly Pro Glu Leu Asp Val Ser Ala Pro Gly Thr Ser Ile Leu Ser Thr 
        195                 200                 205             
Val Pro Ser Ser Gly Tyr Thr Ser Tyr Thr Gly Thr Ser Met Ala Ser 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys Asn Pro Asn 
225                 230                 235                 240 
Leu Thr Asn Ser Gln Val Arg Gln Arg Leu Glu Asn Thr Ala Thr Pro 
                245                 250                 255     
Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ser Asn 
        275 

<210>  2
<211>  275
<212>  PRT
<213>  （人工序列）

<400>  2
Ala Gln Thr Val Pro Tyr Gly Ile Pro Gln Ile Lys Ala Pro Ala Val 
1               5                   10                  15      
His Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile His Ala Ala His Pro Asp Leu Asn Val Ala Gly Gly Ala 
        35                  40                  45              
Ser Phe Val Pro Ser Glu Pro Asn Ala Thr Gln Asp Phe Gln Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asp Asn Thr Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Arg Tyr Gly Asp Gly Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu 
            100                 105                 110         
Trp Ala Val Ala Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Ser Gly Ser Thr Ala Leu Lys Asn Thr Val Asp Thr Ala Asn Asn 
    130                 135                 140                 
Arg Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Ser Ser Thr Val Gly Tyr Pro Ala Lys Tyr Asp Ser Thr Ile Ala 
                165                 170                 175     
Val Ala Asn Val Asn Ser Asn Asn Val Arg Asn Ser Ser Ser Ser Ala 
            180                 185                 190         
Gly Pro Glu Leu Asp Val Ser Ala Pro Gly Thr Ser Ile Leu Ser Thr 
        195                 200                 205             
Val Pro Ser Ser Gly Tyr Thr Ser Ser Thr Gly Thr Ser Met Ala Ser 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys Asn Pro Asn 
225                 230                 235                 240 
Leu Thr Asn Ser Gln Val Arg Gln Arg Leu Glu Asn Thr Ala Thr Pro 
                245                 250                 255     
Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ser Asn 
        275 

<210>  3
<211>  275
<212>  PRT
<213>  （人工序列）

<400>  3
Ala Gln Thr Val Pro Tyr Gly Ile Pro Gln Ile Lys Ala Pro Ala Val 
1               5                   10                  15      
His Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile His Ala Ala His Pro Asp Leu Asn Val Ala Gly Gly Ala 
        35                  40                  45              
Ser Phe Val Pro Ser Glu Pro Asn Ala Thr Gln Asp Phe Gln Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asp Asn Thr Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Arg Tyr Gly Asp Gly Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu 
            100                 105                 110         
Trp Ala Val Ala Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Ser Gly Ser Thr Ala Leu Lys Asn Thr Val Asp Thr Ala Asn Asn 
    130                 135                 140                 
Arg Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Ser Ser Thr Val Gly Tyr Pro Ala Lys Tyr Asp Ser Thr Ile Ala 
                165                 170                 175     
Val Ala Asn Val Asn Ser Asn Asn Val Arg Asn Ser Ser Ser Ser Ala 
            180                 185                 190         
Gly Pro Glu Leu Asp Val Ser Ala Pro Gly Thr Ser Ile Leu Ser Thr 
        195                 200                 205             
Val Pro Ser Ser Gly Tyr Thr Ser Tyr Thr Gly Thr Ser Met Ala Ser 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Cys Lys Asn Pro Asn 
225                 230                 235                 240 
Leu Thr Asn Ser Gln Val Arg Gln Arg Leu Glu Asn Thr Ala Thr Pro 
                245                 250                 255     
Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ser Asn 
        275 

<210>  4
<211>  275
<212>  PRT
<213>  （人工序列）

<400>  4
Ala Gln Thr Val Pro Tyr Gly Ile Pro Gln Ile Lys Ala Pro Ala Val 
1               5                   10                  15      
His Ala Gln Gly Tyr Lys Gly Ala Asn Val Lys Val Ala Val Leu Asp 
            20                  25                  30          
Thr Gly Ile His Ala Ala His Pro Asp Leu Asn Val Ala Gly Gly Ala 
        35                  40                  45              
Ser Phe Val Pro Ser Glu Pro Asn Ala Thr Gln Asp Phe Gln Ser His 
    50                  55                  60                  
Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asp Asn Thr Ile Gly 
65                  70                  75                  80  
Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      
Asp Arg Tyr Gly Asp Gly Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu 
            100                 105                 110         
Trp Ala Val Ala Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             
Pro Ser Gly Ser Thr Ala Leu Lys Asn Thr Val Asp Thr Ala Asn Asn 
    130                 135                 140                 
Arg Gly Val Val Val Val Ala Ala Ala Gly Asn Ser Gly Ser Ser Gly 
145                 150                 155                 160 
Ser Ser Ser Thr Val Gly Tyr Pro Ala Lys Tyr Asp Ser Thr Ile Ala 
                165                 170                 175     
Val Ala Asn Val Asn Ser Asn Asn Val Arg Asn Ser Ser Ser Ser Ala 
            180                 185                 190         
Gly Pro Glu Leu Asp Val Ser Ala Pro Gly Thr Ser Ile Leu Ser Thr 
        195                 200                 205             
Val Pro Ser Ser Gly Tyr Thr Ser Tyr Thr Gly Thr Ser Met Ala Ser 
    210                 215                 220                 
Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys Asn Pro Asn 
225                 230                 235                 240 
Leu Thr Asn Ser Gln Val Arg Gln Arg Leu Glu Asn Thr Ala Thr Pro 
                245                 250                 255     
Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         
Ala Ser Asn 
        275 

<210>  5
<211>  22
<212>  DNA
<213>  （人工序列）

<400>  5
ttgccaatgt agatagtaac aa                                                 22

<210>  6
<211>  24
<212>  DNA
<213>  （人工序列）

<400>  6
tgacattgtt actatctaca ttgg                                               24

<210>  7
<211>  24
<212>  DNA
<213>  （人工序列）

<400>  7
ggatacactt cttatagcgg aaca                                               24

<210>  8
<211>  24
<212>  DNA
<213>  （人工序列）
<400>  8
ccatagatgt tccgctataa gaag                                               24

<210>  9
<211>  24
<212>  DNA
<213>  （人工序列）

<400>  9
cagcgcttat tctttgcaaa aacc                                               24

<210>  10
<211>  23
<212>  DNA
<213>  （人工序列）
<400>  10
ttcgggtttt tgcaaagaat aag                                                23

<210>  11
<211>  22
<212>  DNA
<213>  （人工序列）

<400>  11
ttgccaatgt agatagtaac aa                                                 22

<210>  12
<211>  24
<212>  DNA
<213>  （人工序列）

<400>  12
tgacattgtt actatctaca ttgg                                               24

<210>  13
<211>  24
<212>  DNA
<213>  （人工序列）

<400>  13
cagcgcttat tctttgcaaa aacc                                               24

<210>  14
<211>  23
<212>  DNA
<213>  （人工序列）

<400>  14
ttcgggtttt tgcaaagaat aag                                                23

